CN105708882A - Extraction process of sweet potato fol. flavone - Google Patents
Extraction process of sweet potato fol. flavone Download PDFInfo
- Publication number
- CN105708882A CN105708882A CN201410734835.XA CN201410734835A CN105708882A CN 105708882 A CN105708882 A CN 105708882A CN 201410734835 A CN201410734835 A CN 201410734835A CN 105708882 A CN105708882 A CN 105708882A
- Authority
- CN
- China
- Prior art keywords
- flavone
- ethanol
- folium ipomoea
- sweet potato
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses an extraction process of sweet potato fol. flavone, and relates to the technical field of plant extraction. The extraction process comprises the following steps: drying sweet potato leaves at 50-60 DEG C, crushing the dried sweet potato leaves and filtering the crushed sweet potato leaves by virtue of a 60-mesh sieve; extracting the filtered sweet potato leaves for 2-5 times by taking 60-75%v/v of an ethanol solution as a solvent by virtue of an approach of reflux extraction, wherein each time of extraction lasts for 1-3h, so that extracting solutions are obtained, combining the extracting solutions and recovering ethanol, and concentrating the combined extracting solutions until specific gravity is 1-2, so that total sweet potato fol. flavone is obtained; adding water to the total sweet potato fol. flavone, so that a solution of 0.2-0.5mg/ml is obtained, regulating pH value of the solution to 3-4 and filtering by virtue of macro-porous resin; then, washing the macro-porous resin by virtue of water; then, eluting by virtue of ethanol; and collecting an eluent, and drying so as to obtain the sweet potato fol. flavone. The extraction process disclosed by the invention is simple, low in cost and high in extraction rate; based upon high performance liquid chromatography, the flavone is high in content; and the flavone is relatively high in bioactivity and is quite high in application value.
Description
Technical field
The present invention relates to technical field of plant extraction, especially a kind of technique extracting flavone for raw material with Folium Ipomoea.
Background technology
Radix Ipomoeae is one of Four main crop of extensively cultivating of China, and resource is very abundant.Recent studies indicate that, Folium Ipomoea has following biological activity: Folium Ipomoea contains Insulin-Like composition to be had therapeutical effect, the inhibitory action of pathogenic bacterium, anti-tumor activity, ant-imutagenicity, enhancing platelet and anastalsis, enhancing immunization, blood fat reducing, cholesterol reducing effect, scavenging free radicals, protection vision etc. are acted on diabetes.Containing higher flavone compound in Folium Ipomoea, it it is one of its primary bioactive components.After the 1950's, the research of flavone compound is increasingly turned in its scavenging free radicals defying age and the prophylactic-therapeutic effect to Senile disease, evaluates and screening is rich in one of the plant resources study hotspot having become agronomy, medical science, Food Science of flavone compound.
At present, Folium Ipomoea is except part is edible or makes animal feeding-stuff, and the overwhelming majority is dropped, and causes the significant wastage of resource.Therefore, it is necessary to develop the technology made new advances to extract flavonoid anti-oxidizing compounds from cheap Folium Ipomoea, strengthen the comprehensive utilization of Folium Ipomoea, turn waste into wealth, improve added value.
Summary of the invention
The goal of the invention of the present invention is in that: provide the extraction process of a kind of Folium Ipomoea flavone for the problems referred to above, this extraction process is simple, cost is low, extraction ratio is high.
For achieving the above object, the technical solution used in the present invention is as follows:
The extraction process of a kind of Folium Ipomoea flavone, comprises the following steps:
(1) take Folium Ipomoea in 50-60 DEG C of drying, cross 60 mesh sieves after pulverizing and obtain Folium Ipomoea powder；
(2) with the alcoholic solution of 60-75%v/v for solvent, soak with ethanol 2-3 hour of 2-6 times of Folium Ipomoea powder weight is added；At 60-80 DEG C, adding alcohol reflux lixiviate 2-5 time of 2-4 times of Folium Ipomoea powder weight, each lixiviate 1-3 hour, prepare extracting solution, united extraction liquid also reclaims ethanol, then extracting solution is concentrated into proportion is 1-2, prepares Folium Ipomoea total flavones；
(3) purification: added water by Folium Ipomoea total flavones and be configured to the solution of 0.2-0.5mg/ml, the pH value of solution is adjusted to 3-4, crosses the macroporous resin after cleaning with the speed of 6-8BV/h；Total flavones solution adopts the consumption water more than 3BV to clean macroporous resin after having crossed；To adopt consumption again be 3-6BV, concentration is the ethanol elution of 30%-50%v/v；Collect eluent, dry, obtain Folium Ipomoea flavone.
Further, the cleaning treatment method of described macroporous resin is: macroporous resin is first with 95%v/v soak with ethanol 24h, wet method dress post, flow on post eluting with the 95%v/v ethanol of 4BV, being washed till the volume ratio that ethanol mixes with water is 1:5, no longer have till white casse, then wash away ethanol with deionized water, until making eluent without alcohol taste.
Further, described macroporous resin is HPD-500 or HPD-600.
In sum, owing to have employed technique scheme, the invention has the beneficial effects as follows: Folium Ipomoea extracting flavonoids technique provided by the present invention is simple, cost is low, extraction ratio is high, show that this flavones content is high through efficient liquid phase chromatographic analysis, and there is higher biological activity, therefore there is significantly high using value.
Detailed description of the invention
Below by way of detailed description of the invention, the invention will be further described.
Embodiment 1
The extraction process of a kind of Folium Ipomoea flavone, comprises the following steps:
(1) take Folium Ipomoea in 50 DEG C of drying, cross 60 mesh sieves after pulverizing and obtain Folium Ipomoea powder；
(2) with the alcoholic solution of 60%v/v for solvent, the soak with ethanol of 2 times of Folium Ipomoea powder weight of addition 2 hours；At 60 DEG C, adding the alcohol reflux lixiviate 2 times of 2 times of Folium Ipomoea powder weight, each lixiviate 1 hour, prepare extracting solution, united extraction liquid also reclaims ethanol, then extracting solution is concentrated into proportion is 1, prepares Folium Ipomoea total flavones；
(3) purification: be first carried out HPD-500 macroporous resin processing, adopt 95%v/v soak with ethanol 24h, wet method dress post, flow on post eluting with the 95%v/v ethanol of 4BV, being washed till the volume ratio that ethanol mixes with water is 1:5, no longer have till white casse, then wash away ethanol with deionized water, until making eluent without alcohol taste.Then Folium Ipomoea total flavones is added water and be configured to the solution of 0.2mg/ml, the pH value of solution is adjusted to 3, cross the macroporous resin after cleaning with the speed of 6BV/h；Total flavones solution adopts the consumption water more than 3BV to clean macroporous resin after having crossed；To adopt consumption again be 3BV, concentration is the ethanol elution of 30%v/v；Collect eluent, dry, obtain the Folium Ipomoea flavone of purification.
Embodiment 2
The extraction process of a kind of Folium Ipomoea flavone, comprises the following steps:
(1) take Folium Ipomoea in 60 DEG C of drying, cross 60 mesh sieves after pulverizing and obtain Folium Ipomoea powder；
(2) with the alcoholic solution of 75%v/v for solvent, the soak with ethanol of 6 times of Folium Ipomoea powder weight of addition 2 hours；At 80 DEG C, adding the alcohol reflux lixiviate 5 times of 4 times of Folium Ipomoea powder weight, each lixiviate 3 hours, prepare extracting solution, united extraction liquid also reclaims ethanol, then extracting solution is concentrated into proportion is 2, prepares Folium Ipomoea total flavones；
(3) purification: be first carried out HPD-500 macroporous resin processing, adopt 95%v/v soak with ethanol 24h, wet method dress post, flow on post eluting with the 95%v/v ethanol of 4BV, being washed till the volume ratio that ethanol mixes with water is 1:5, no longer have till white casse, then wash away ethanol with deionized water, until making eluent without alcohol taste.Then Folium Ipomoea total flavones is added water and be configured to the solution of 0.5mg/ml, the pH value of solution is adjusted to 4, cross the macroporous resin after cleaning with the speed of 8BV/h；The water that total flavones solution has adopted consumption to be 4BV after having crossed cleans macroporous resin；To adopt consumption again be 6BV, concentration is the ethanol elution of 50%v/v；Collect eluent, dry, obtain the Folium Ipomoea flavone of purification.
Embodiment 3
The extraction process of a kind of Folium Ipomoea flavone, comprises the following steps:
(1) take Folium Ipomoea in 55 DEG C of drying, cross 60 mesh sieves after pulverizing and obtain Folium Ipomoea powder；
(2) with the alcoholic solution of 65%v/v for solvent, the soak with ethanol of 3 times of Folium Ipomoea powder weight of addition 2 hours；At 70 DEG C, adding the alcohol reflux lixiviate 4 times of 3 times of Folium Ipomoea powder weight, each lixiviate 2 hours, prepare extracting solution, united extraction liquid also reclaims ethanol, then extracting solution is concentrated into proportion is 1.5, prepares Folium Ipomoea total flavones；
(3) purification: be first carried out HPD-500 macroporous resin processing, adopt 95%v/v soak with ethanol 24h, wet method dress post, flow on post eluting with the 95%v/v ethanol of 4BV, being washed till the volume ratio that ethanol mixes with water is 1:5, no longer have till white casse, then wash away ethanol with deionized water, until making eluent without alcohol taste.Then Folium Ipomoea total flavones is added water and be configured to the solution of 0.3mg/ml, the pH value of solution is adjusted to 3.42, cross the macroporous resin after cleaning with the speed of 7BV/h；The water that total flavones solution has adopted consumption to be 5BV after having crossed cleans macroporous resin；To adopt consumption again be 5BV, concentration is the ethanol elution of 40%v/v；Collect eluent, dry, obtain the Folium Ipomoea flavone of purification.
Embodiment 4
The extraction process of a kind of Folium Ipomoea flavone, comprises the following steps:
(1) take Folium Ipomoea in 56 DEG C of drying, cross 60 mesh sieves after pulverizing and obtain Folium Ipomoea powder；
(2) with the alcoholic solution of 75%v/v for solvent, the soak with ethanol of 5 times of Folium Ipomoea powder weight of addition 2 hours；At 75 DEG C, adding the alcohol reflux lixiviate 3 times of 3 times of Folium Ipomoea powder weight, each lixiviate 3 hours, prepare extracting solution, united extraction liquid also reclaims ethanol, then extracting solution is concentrated into proportion is 1.2, prepares Folium Ipomoea total flavones；
(3) purification: be first carried out HPD-600 macroporous resin processing, adopt 95%v/v soak with ethanol 24h, wet method dress post, flow on post eluting with the 95%v/v ethanol of 4BV, being washed till the volume ratio that ethanol mixes with water is 1:5, no longer have till white casse, then wash away ethanol with deionized water, until making eluent without alcohol taste.Then Folium Ipomoea total flavones is added water and be configured to the solution of 0.35mg/ml, the pH value of solution is adjusted to 3.8, cross the macroporous resin after cleaning with the speed of 7BV/h；The water that total flavones solution has adopted consumption to be 6BV after having crossed cleans macroporous resin；To adopt consumption again be 6BV, concentration is the ethanol elution of 30%v/v；Collect eluent, dry, obtain the Folium Ipomoea flavone of purification.
Folium Ipomoea extracting flavonoids technique provided by the present invention is simple, cost is low, extraction ratio is high, shows that this flavones content is high through efficient liquid phase chromatographic analysis, and has higher biological activity, has significantly high using value.
Claims (3)
1. the extraction process of a Folium Ipomoea flavone, it is characterised in that comprise the following steps:
(1) take Folium Ipomoea in 50-60 DEG C of drying, cross 60 mesh sieves after pulverizing and obtain Folium Ipomoea powder；
(2) with the alcoholic solution of 60-75%v/v for solvent, soak with ethanol 2-3 hour of 2-6 times of Folium Ipomoea powder weight is added；At 60-80 DEG C, adding alcohol reflux lixiviate 2-5 time of 2-4 times of Folium Ipomoea powder weight, each lixiviate 1-3 hour, prepare extracting solution, united extraction liquid also reclaims ethanol, then extracting solution is concentrated into proportion is 1-2, prepares Folium Ipomoea total flavones；
(3) purification: added water by Folium Ipomoea total flavones and be configured to the solution of 0.2-0.5mg/ml, the pH value of solution is adjusted to 3-4, crosses the macroporous resin after cleaning with the speed of 6-8BV/h；Total flavones solution adopts the consumption water more than 3BV to clean macroporous resin after having crossed；To adopt consumption again be 3-6BV, concentration is the ethanol elution of 30%-50%v/v；Collect eluent, dry, obtain Folium Ipomoea flavone.
2. the extraction process of a kind of Folium Ipomoea flavone according to claim 1, it is characterized in that: the cleaning treatment method of described macroporous resin is: macroporous resin is first with 95%v/v soak with ethanol 24h, wet method dress post, flow on post eluting with the 95%v/v ethanol of 4BV, being washed till the volume ratio that ethanol mixes with water is 1:5, no longer have till white casse, then wash away ethanol with deionized water, until making eluent without alcohol taste.
3. the extraction process of a kind of Folium Ipomoea flavone according to claim 2, it is characterised in that: described macroporous resin is HPD-500 or HPD-600.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410734835.XA CN105708882A (en) | 2014-12-05 | 2014-12-05 | Extraction process of sweet potato fol. flavone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410734835.XA CN105708882A (en) | 2014-12-05 | 2014-12-05 | Extraction process of sweet potato fol. flavone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105708882A true CN105708882A (en) | 2016-06-29 |
Family
ID=56144051
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410734835.XA Pending CN105708882A (en) | 2014-12-05 | 2014-12-05 | Extraction process of sweet potato fol. flavone |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105708882A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106511435A (en) * | 2016-12-17 | 2017-03-22 | 王先涛 | Method for using spirulina maxima for increasing sweet potato stem and leaf flavone extraction ratio |
| CN108925926A (en) * | 2018-08-23 | 2018-12-04 | 河南农业大学 | A kind of production method of sweet potato leaves jelly |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167781A (en) * | 2007-11-09 | 2008-04-30 | 燕山大学 | Orally-administered hypoglycemic sweet potato leaf single prescription traditional Chinese medicine and preparation method thereof |
-
2014
- 2014-12-05 CN CN201410734835.XA patent/CN105708882A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167781A (en) * | 2007-11-09 | 2008-04-30 | 燕山大学 | Orally-administered hypoglycemic sweet potato leaf single prescription traditional Chinese medicine and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 赵玉芬 等: ""大孔树脂对红薯叶总黄酮的吸附及解吸特性研究"", 《生物技术进展》 * |
| 陆英 等: ""红薯叶黄酮分离纯化工艺及抗氧化性研究"", 《食品科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106511435A (en) * | 2016-12-17 | 2017-03-22 | 王先涛 | Method for using spirulina maxima for increasing sweet potato stem and leaf flavone extraction ratio |
| CN108925926A (en) * | 2018-08-23 | 2018-12-04 | 河南农业大学 | A kind of production method of sweet potato leaves jelly |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102451235B (en) | Preparation method of olive leaf extract | |
| CN101773593B (en) | Method for preparing antioxidative active extractive of sweet potato leaves | |
| CN104311676B (en) | A kind of extraction food starch method of by-product tannic acid from rubber seed core | |
| CN103393780A (en) | Extraction method of high-purity coptis total alkaloid | |
| CN101463027B (en) | Method for extracting and separating flavone and anthocyanidin from elder fruit | |
| CN102349951A (en) | Preparation method of hawthorn leaf extract | |
| CN101322737B (en) | Persimmon leaf flavones extract and preparation thereof | |
| CN105616465A (en) | Method for extracting polyphenol from mango skins | |
| CN104262446B (en) | The method of mogroside Ⅴ is extracted from Grosvenor Momordica | |
| CN106083799A (en) | A kind of method preparing different purity and the procyanidin without aflatoxin | |
| CN105795095B (en) | Preparation method of cardamine violifolia selenoprotein with low heavy metal content | |
| CN105169094B (en) | Indocalamus leaf total flavone extracting and purifying method | |
| CN103275175B (en) | The preparation method of dandelion small molecule glycoprotein | |
| CN103494858A (en) | Method for enriching total flavonoids in taraxacum mongolicum with macroporous resins | |
| CN104356105B (en) | A kind of preparation method of EGCG | |
| CN101584752B (en) | Extraction and purification process for total flavonoids in Anchusa italica Retiz | |
| CN105732741A (en) | Method for extracting anthocyanin and ursolic acid from perilla leaves | |
| CN105708882A (en) | Extraction process of sweet potato fol. flavone | |
| CN105884754A (en) | Fine extraction method of silibinin | |
| CN103408610A (en) | Method for extracting arbutin from pear leaves | |
| CN102648965A (en) | Industrialization preparation method of lophatherum gracile general flavone | |
| CN102302539A (en) | Method for producing trifolium pratense L. isoflavones | |
| CN107556194A (en) | The method of chlorogenic acid extracting from desmodium | |
| CN104230871B (en) | A kind of method separating polymethoxyflavone in Fructus Aurantii Immaturus, Hesperidin and Neosynephrine | |
| CN103585424A (en) | Method for extracting total flavone from Chinese chive seeds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160629 |