CN102579511A - Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus - Google Patents
Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus Download PDFInfo
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Abstract
The invention relates to a method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus. The method mainly comprises the following steps: high-pressure treatment, ethanol extraction, concentration, centrifugation, deionized water extraction, concentration, ethanol precipitation, separation, purification, freezing and drying. Compared with the conventional extraction method, the provided method has the biggest difference in that high-pressure treatment is carried out to the raw material for preliminary treatment under the appropriate time condition, then an extract containing steroids is obtained by an ethanol extraction method, the steroidal compounds are purified by a water washing method and a centrifugation method, extracts of polysaccharides and polyphenols are obtained by a water extraction method, and then the polysaccharides and polyphenols are separated through ethanol precipitation. The tissue structure of inonotus obliquus changes due to high-pressure treatment, therefore, the yield of the steroidal compounds, polysaccharides and polyphenols is high, the molecular structure of active components in inonotus obliquus changes under high pressure, and the study verifies that the activity of inonotus obliquus can be enhanced.
Description
Technical field
The present invention relates to the method for distilling of a kind of steroid compound, polysaccharide and polyphenol, particularly relate to a kind of method of from Inonqqus obliquus, comprehensively extracting steroid compound, polysaccharide and polyphenol.
Background technology
Inonqqus obliquus belongs to Eumycota, Basidiomycotina, and Hymenomycetes, non-brown Zoopagales, rust leather pore fungi section, brown transverse hole fungus belongs to, formal name used at school Phaeoporousobliquus (per:Fr.) J.Schroet.The chemical composition of Inonqqus obliquus mainly contains vanillic acid, syringic acid and the gamma-hydroxy formic acid etc. of lanoline alkane type steroid compound, polysaccharide, Polyphenols, lignin, melanin, alkaloids, tannin chemical compound, folic acid derivatives and fragrance at present.At present, relevant research worker has been launched research to the biological activity of these compositions, and is applied in the prevention and treatment of malignant tumor, diabetes, cardiovascular disease, hepatopathy and AIDS.
Steroidal class material is a kind of main active component in the Inonqqus obliquus, and its content is big, and active high, Inonqqus obliquus alcohol then is wherein a kind of main chemical compound, discovers that it has good antitumor activity, therefore receives in recent years widely and paying close attention to.
Fuscoporia obliqua polysaccharide is another the important active skull cap components in the Inonqqus obliquus, has different physiological roles and to human non-toxic, therefore also receives concern widely in recent years.Discover that Fuscoporia obliqua polysaccharide has good antioxidation, blood sugar lowering, antitumor and immunoregulation effect, especially significant blood sugar decreasing effect and immunocompetence make it be expected to become the natural drug of prevent diabetes and cardiovascular disease, enhancing immunity.
The Inonqqus obliquus polyphenol is the natural product that from Inonqqus obliquus, extracts; Because the Inonqqus obliquus polyphenol is a kind of natural anti-oxidation antistaling agent of high-efficiency low-toxicity; Thereby in food industry, be mainly used in the fresh-keeping of oils and fats and goods thereof, fried and baked goods, fish and meat products, and can replace synthetic antioxidant BHA, BHT commonly used in the present food industry.In addition; The Inonqqus obliquus polyphenol has antiviral, antibiotic, antibacterial, anti-caries aspect medical, improves renal function, blood fat reducing, blood pressure lowering, blood sugar lowering, antioxidation, effect such as anticancer, and clinical research finds that the Inonqqus obliquus polyphenol has good action to alleviating the cardiovascular system pathological changes.
Adopt at present usually water extraction ethanol precipitation or solvent-extracted method to carry out the extraction of polysaccharide and polyphenol in the Inonqqus obliquus and separate; Because conventional water extraction ethanol precipitation method for distilling yield is lower; The Fuscoporia obliqua polysaccharide yield has only 2%～5%; Inonqqus obliquus polyphenol yield is lower, and the solvent extraction rule can cause environmental pollution because of having used a large amount of organic solvents.And up to the present do not see a kind of report and instance that can extract the method for steroid compound, polysaccharide and polyphenol in the Inonqqus obliquus simultaneously as yet.
Summary of the invention
In order to address the above problem, the object of the present invention is to provide the high comprehensive method of extracting steroid compound, polysaccharide and polyphenol from Inonqqus obliquus of a kind of product yield.
In order to achieve the above object, provided by the invention from Inonqqus obliquus the comprehensive method of extracting steroid compound, polysaccharide and polyphenol comprise the following step that carries out in order:
1) Inonqqus obliquus is ground into powder in electric mill, handled 1～3 hour at the high-pressure sterilizing pot mesohigh then, so that raw material is carried out pre-treatment;
2) above-mentioned Inonqqus obliquus through HIGH PRESSURE TREATMENT is joined in the ethanol of 80% concentration; Said Inonqqus obliquus and alcoholic acid mass ratio are 1: 10～1: 20; Extracted 1～2 hour 80 ℃～90 ℃ temperature refluxed then; Lixiviate is 3 times repeatedly, and filtration under diminished pressure afterwards merges filtrating and obtain containing the Inonqqus obliquus test liquid of steroid compound;
3) under 40 ℃～60 ℃ temperature and the pressure of 0.06～0.1MPa, place the Rotary Evaporators vacuum rotary steam to there not being ethanol the above-mentioned Inonqqus obliquus test liquid that contains steroid compound; Using volume is the water suspendible washing of 5～10 times of amounts of above-mentioned concentrated back Inonqqus obliquus test liquid; Centrifugalize afterwards, the collecting precipitation thing;
4) the above-mentioned drying precipitate of collecting is promptly obtained the steroid compound product;
5) in step 2) in add deionized water in the residue after extracting; The mass ratio of said Inonqqus obliquus residue and deionized water is 1: 10～1: 30; Extracted 1～2 hour 90 ℃～100 ℃ temperature refluxed then; Lixiviate is 3 times repeatedly, and filtration under diminished pressure afterwards merges filtrating and obtain containing the Inonqqus obliquus test liquid of Fuscoporia obliqua polysaccharide and polyphenol;
6) under 40 ℃～60 ℃ temperature and the pressure of 0.06～0.1MPa, place Rotary Evaporators to be evaporated to 1/10～1/4 of original volume the above-mentioned Inonqqus obliquus test liquid that contains Fuscoporia obliqua polysaccharide and polyphenol; In concentrated solution, adding ethanol to ethanol mass concentration then precipitates more than 70%; Centrifugalize afterwards, collecting precipitation thing and supernatant respectively; Dehydrated alcohol with 2～3 times of volumes washs decolouring 3～5 times to above-mentioned precipitate then, and secondary precipitate is collected in centrifugalize afterwards;
7) secondary precipitate of collecting in the step 6) is drying to obtain the Fuscoporia obliqua polysaccharide product;
8) supernatant concentration that obtains in the step 6) is not distinguished the flavor of to there being alcohol, adopt the method that dynamically goes up appearance then, last kind to the good macroporous resin pillar of pretreatment; Water and 30%～50% mass concentration ethanol carried out gradient elution after last appearance was accomplished;
9) under 30 ℃～40 ℃ temperature and the pressure of 0.06～0.1MPa with step 8) in 30%～50% mass concentration ethanol elution place the Rotary Evaporators concentrating under reduced pressure not have alcohol flavor, be drying to obtain Inonqqus obliquus polyphenol product then.
The pressure that described step 1) mesohigh is handled is 100KPA, and temperature is 121 ℃.
Macroporous resin is D101 in the described step 8), and last appearance flow velocity is 2～3 times of column volume/h; The elution amount of each gradient is 3 times of column volume～4 times column volumes.
Described step 4), 7) and 9) in the dry device that is adopted be freezer dryer, air drier or vacuum drier.
The maximum difference of the method for from Inonqqus obliquus, extracting steroid compound, polysaccharide and polyphenol provided by the invention and existing method for distilling is that the present invention adopts high-pressure sterilizing pot under suitable time conditions, raw material to be carried out HIGH PRESSURE TREATMENT earlier; So that raw material is carried out pre-treatment; Utilize ethanol extraction method to obtain to contain the extract of steroidal class then; Reuse washing and centrifugation method purification steroid compound are with the extract of water extraction acquisition polysaccharide and polyphenol, precipitate with ethanol separating polyose and polyphenol then.Because HIGH PRESSURE TREATMENT makes the Inonqqus obliquus organizational structure change, thereby the yield of steroid compound, polysaccharide and polyphenol is high, and under highly compressed effect, the structure of active component molecule can change in the Inonqqus obliquus, can make its increased activity.In addition, because Inonqqus obliquus is to treasure resource, adopt this method can promote the comprehensive utilization of resource.In addition, this method also has advantage of simple technology.
Description of drawings
Fig. 1 extracts steroid compound, polysaccharide and polyphenol yield rectangular histogram for utilizing method provided by the invention and existing method for distilling from Inonqqus obliquus.
Fig. 2 is that the steroid compound, polysaccharide and the polyphenol that utilize method provided by the invention and existing method for distilling from Inonqqus obliquus, to extract suppress malonaldehyde (MDA) generative capacity experimental result rectangular histogram.
Fig. 3 is steroid compound, polysaccharide and a polyphenol reducing power ability experimental result rectangular histogram of utilizing method provided by the invention and existing method for distilling from Inonqqus obliquus, to extract.
The specific embodiment
Below in conjunction with accompanying drawing and specific embodiment steroid compound, polysaccharide and the polyphenol method from Inonqqus obliquus, extracted provided by the invention is elaborated.
Embodiment 1:
1) Inonqqus obliquus is ground into powder in electric mill, (100KPA, 121 ℃) HIGH PRESSURE TREATMENT 1 hour in high-pressure sterilizing pot then is to carry out pre-treatment to raw material;
2) above-mentioned Inonqqus obliquus through HIGH PRESSURE TREATMENT is joined in the ethanol of 80% mass concentration; Said Inonqqus obliquus and alcoholic acid mass ratio are 1: 20; Extracted 2 hours 90 ℃ temperature refluxed then; Lixiviate is 3 times repeatedly, and filtration under diminished pressure afterwards merges filtrating and obtain containing the Inonqqus obliquus test liquid of steroid compound;
3) under the pressure of 60 ℃ temperature and 0.1MPa, place the Rotary Evaporators vacuum rotary steam to there not being ethanol the above-mentioned Inonqqus obliquus test liquid that contains steroid compound; Using volume is the water suspendible washing of 10 times of amounts of above-mentioned concentrated back Inonqqus obliquus test liquid; Centrifugalize afterwards, the collecting precipitation thing;
4) precipitate of above-mentioned collection is carried out drying with freezer dryer, air drier or vacuum drier and can obtain the steroid compound series products, its yield is 0.96%, and using vanillin-ice acetic acid method to record its steroidal content is 78.20%;
5) in step 2) in add deionized water in the residue after extracting; The mass ratio of said Inonqqus obliquus residue and deionized water is 1: 30; Extracted 2 hours 100 ℃ temperature refluxed then; Lixiviate is 3 times repeatedly, and filtration under diminished pressure afterwards merges filtrating and obtain containing the Inonqqus obliquus test liquid of Fuscoporia obliqua polysaccharide and polyphenol;
6) under the pressure of 60 ℃ temperature and 0.1MPa, place Rotary Evaporators to be evaporated to 1/4 of original volume the above-mentioned Inonqqus obliquus test liquid that contains Fuscoporia obliqua polysaccharide and polyphenol; In concentrated solution, adding ethanol to ethanol mass concentration then precipitates more than 70%; Centrifugalize afterwards, collecting precipitation thing and supernatant respectively; Dehydrated alcohol with 3 times of volumes washs decolouring 5 times to above-mentioned precipitate then, and secondary precipitate is collected in centrifugalize afterwards;
7) secondary precipitate of collecting in the step 6) is carried out drying with freezer dryer, air drier or vacuum drier and can obtain the Fuscoporia obliqua polysaccharide product, its yield is 7.69%, and using sulfuric acid-phynol method to record its polyoses content is 43.73%;
8) with the supernatant concentration that obtains in the step 6) to nothing alcohol flavor, adopt dynamically the upward method of appearance then, last appearance is to the good D101 macroporous resin pillar of pretreatment, and last appearance flow velocity is 3 times of column volume/h; Water and 30% ethanol carried out eluting, 4 times of column volumes of each gradient elution after last appearance was accomplished;
9) under the pressure of 40 ℃ temperature and 0.1MPa with step 8) in 30% mass concentration ethanol elution place the Rotary Evaporators concentrating under reduced pressure not have the alcohol flavor, can obtain Inonqqus obliquus polyphenol product with dryings such as freezer dryer, air drier or vacuum driers then.Content through Folin-Ciocalteu colorimetric method for determining polyphenol is 25.40%, and yield is 1.65%.
Embodiment 2:
1) with Inonqqus obliquus pulverize in electric mill, (100KPA, 121 ℃) HIGH PRESSURE TREATMENT 2 hours in high-pressure sterilizing pot then is to carry out pre-treatment to raw material;
2) above-mentioned Inonqqus obliquus through HIGH PRESSURE TREATMENT is joined in the ethanol of 80% mass concentration; Said Inonqqus obliquus and alcoholic acid mass ratio are 1: 15; Extracted 1.5 hours 80 ℃ temperature refluxed then; Lixiviate is 3 times repeatedly, and filtration under diminished pressure afterwards merges filtrating and obtain containing the Inonqqus obliquus test liquid of steroid compound;
3) under the pressure of 50 ℃ temperature and 0.08MPa, place the Rotary Evaporators vacuum rotary steam to there not being ethanol the above-mentioned Inonqqus obliquus test liquid that contains steroid compound; Using volume is the water suspendible washing of 7 times of amounts of above-mentioned concentrated back Inonqqus obliquus test liquid; Centrifugalize afterwards, the collecting precipitation thing;
4) precipitate of above-mentioned collection is carried out drying with freezer dryer, air drier or vacuum drier and can obtain the steroid compound series products, its yield is 1.06%, and using vanillin-ice acetic acid method to record its steroidal content is 83.97%;
5) in step 2) in add deionized water in the residue after extracting; The mass ratio of said Inonqqus obliquus residue and deionized water is 1: 20; Extracted 1.5 hours 80 ℃ temperature refluxed then; Lixiviate is 3 times repeatedly, and filtration under diminished pressure afterwards merges filtrating and obtain containing the Inonqqus obliquus test liquid of Fuscoporia obliqua polysaccharide and polyphenol;
6) under the pressure of 50 ℃ temperature and 0.08MPa, place Rotary Evaporators to be evaporated to 1/8 of original volume the above-mentioned Inonqqus obliquus test liquid that contains Fuscoporia obliqua polysaccharide and polyphenol; In concentrated solution, adding ethanol to ethanol mass concentration then precipitates more than 70%; Centrifugalize afterwards, collecting precipitation thing and supernatant respectively; Dehydrated alcohol with 2.5 times of volumes washs decolouring 4 times to above-mentioned precipitate then, and secondary precipitate is collected in centrifugalize afterwards;
7) secondary precipitate of collecting in the step 6) is carried out drying with freezer dryer, air drier or vacuum drier and can obtain the Fuscoporia obliqua polysaccharide product, its yield is 9.00%, and using sulfuric acid-phynol method to record its polyoses content is 43.59%;
8) with the supernatant concentration that obtains in the step 6) to nothing alcohol flavor, adopt dynamically the upward method of appearance then, last appearance is to the good D101 macroporous resin pillar of pretreatment, and last appearance flow velocity is 2.5 times of column volume/h; Water and 50% mass concentration ethanol carried out eluting, 3.5 times of column volumes of each gradient elution after last appearance was accomplished;
9) under the pressure of 35 ℃ temperature and 0.08MPa with step 8) in 50% mass concentration ethanol elution place the Rotary Evaporators concentrating under reduced pressure not have the alcohol flavor, carry out drying with freezer dryer, air drier or vacuum drier then and can obtain Inonqqus obliquus polyphenol product.Content through Folin-Ciocalteu colorimetric method for determining polyphenol is 20.27%, and yield is 1.22%.
Embodiment 3:
1) with Inonqqus obliquus pulverize in electric mill, (100KPA, 121 ℃) HIGH PRESSURE TREATMENT 3h in high-pressure sterilizing pot then is to carry out pre-treatment to raw material;
2) above-mentioned Inonqqus obliquus through HIGH PRESSURE TREATMENT is joined in the ethanol of 80% mass concentration; Said Inonqqus obliquus and alcoholic acid mass ratio are 1: 10; Extracted 1 hour 80 ℃ temperature refluxed then; Lixiviate is 3 times repeatedly, and filtration under diminished pressure afterwards merges filtrating and obtain containing the Inonqqus obliquus test liquid of steroid compound;
3) under the pressure of 40 ℃ temperature and 0.06MPa, place the Rotary Evaporators vacuum rotary steam to there not being ethanol the above-mentioned Inonqqus obliquus test liquid that contains steroid compound; Using volume is the water suspendible washing of 5 times of amounts of above-mentioned concentrated back Inonqqus obliquus test liquid; Centrifugalize afterwards, the collecting precipitation thing;
4) precipitate of above-mentioned collection is carried out drying with freezer dryer, air drier or vacuum drier and can obtain the steroid compound series products, its yield is 0.82%, and using vanillin-ice acetic acid method to record its steroidal content is 81.48%;
5) in step 2) in add deionized water in the residue after extracting; The mass ratio of said Inonqqus obliquus residue and deionized water is 1: 10; Extracted 1 hour 90 ℃ temperature refluxed then; Lixiviate is 3 times repeatedly, and filtration under diminished pressure afterwards merges filtrating and obtain containing the Inonqqus obliquus test liquid of Fuscoporia obliqua polysaccharide and polyphenol;
6) under the pressure of 40 ℃ temperature and 0.06MPa, place Rotary Evaporators to be evaporated to 1/10 of original volume the above-mentioned Inonqqus obliquus test liquid that contains Fuscoporia obliqua polysaccharide and polyphenol; In concentrated solution, adding ethanol to ethanol mass concentration then precipitates more than 70%; Centrifugalize afterwards, collecting precipitation thing and supernatant respectively; Dehydrated alcohol with 3 times of volumes washs decolouring 3 times to above-mentioned precipitate then, and secondary precipitate is collected in centrifugalize afterwards;
7) secondary precipitate of collecting in the step 6) is carried out drying with freezer dryer, air drier or vacuum drier and can obtain the Fuscoporia obliqua polysaccharide product, its yield is 8.03%, and using sulfuric acid-phynol method to record its polyoses content is 42.68%;
8) with the supernatant concentration that obtains in the step 6) to nothing alcohol flavor, adopt dynamically the upward method of appearance then, last appearance is to the good D101 macroporous resin pillar of pretreatment, and last appearance flow velocity is 2 times of column volume/h; Water and 40% mass concentration ethanol carried out eluting, 3 times of column volumes of each gradient elution after last appearance was accomplished;
9) under the pressure of 30 ℃ temperature and 0.06MPa with step 8) in 40% mass concentration ethanol elution place the Rotary Evaporators concentrating under reduced pressure not have the alcohol flavor, carry out drying with freezer dryer, air drier or vacuum drier then and can obtain Inonqqus obliquus polyphenol product.Content through Folin-Ciocalteu colorimetric method for determining polyphenol is 22.85%, and yield is 1.24%.
The method that the inventor will utilize the invention described above to provide reaches existing method for distilling extracts Inonqqus obliquus steroid compound, polysaccharide and polyphenol from Inonqqus obliquus yield drafting pattern, and the result sees Fig. 1.
In addition, the inventor suppresses malonaldehyde (MDA) generative capacity to extraction Inonqqus obliquus steroid compound, polysaccharide and polyphenol method are obtained from Inonqqus obliquus Inonqqus obliquus steroid compound, polysaccharide and the polyphenol that utilizes the foregoing description to provide and the reducing power ability is tested.
It is reported that lipid peroxidation can pair cell cause very big damage, it is a kind of main peroxy radical, MDA be cause human senility, cause a disease, one of carcinogenic key factor.Therefore, the content of measuring MDA not only can reflect the snperoxiaized degree of cytolipin, and can reflect the extent of damage of MDA pair cell.
Obviously between the reducing power of sample and the oxidation resistance direct relation is arranged.The mechanism of the reducing power of sample comes from the chain that interrupts free radical, thereby perhaps stops the formation of peroxy with the precursor reaction of free radical.
Suppressing malonaldehyde (MDA) generative capacity and reducing power ability, to test employed Inonqqus obliquus steroid compound, polysaccharide and polyphenol all are method acquisitions of adopting the HIGH PRESSURE TREATMENT condition, and contrast with Inonqqus obliquus steroid compound, polysaccharide and polyphenol that untreated existing method is obtained.
Suppress malonaldehyde (MDA) generative capacity experimental result and see Fig. 2.The result shows; Compare with Inonqqus obliquus steroid compound, polysaccharide and polyphenol that untreated existing method is obtained, the Inonqqus obliquus steroid compound, polysaccharide and the polyphenol that utilize the inventive method to obtain all obtain enhancing in various degree to the inhibition ability that mice MDA generates.
Reducing power ability experimental result is seen Fig. 3.The result shows that compare with Inonqqus obliquus steroid compound, polysaccharide and polyphenol that untreated existing method is obtained, Inonqqus obliquus steroid compound, polysaccharide and the polyphenol reducing power ability of utilizing the inventive method to obtain all obtain enhancing in various degree.
Claims (4)
1. comprehensive method of extracting steroid compound, polysaccharide and polyphenol from Inonqqus obliquus, it is characterized in that: the described method of from Inonqqus obliquus, extracting steroid compound, polysaccharide and polyphenol comprises the following step that carries out in order:
1) Inonqqus obliquus is ground into powder in electric mill, handled 1～3 hour at the high-pressure sterilizing pot mesohigh then, so that raw material is carried out pre-treatment;
2) above-mentioned Inonqqus obliquus through HIGH PRESSURE TREATMENT is joined in the ethanol of 80% mass concentration; Said Inonqqus obliquus and alcoholic acid mass ratio are 1: 10～1: 20; Extracted 1～2 hour 80 ℃～90 ℃ temperature refluxed then; Lixiviate is 3 times repeatedly, and filtration under diminished pressure afterwards merges filtrating and obtain containing the Inonqqus obliquus test liquid of steroid compound;
3) under 40 ℃～60 ℃ temperature and the pressure of 0.06～0.1MPa, place the Rotary Evaporators vacuum rotary steam to there not being ethanol the above-mentioned Inonqqus obliquus test liquid that contains steroid compound; Using volume is the water suspendible washing of 5～10 times of volumes of above-mentioned concentrated back Inonqqus obliquus test liquid; Centrifugalize afterwards, the collecting precipitation thing;
4) the above-mentioned drying precipitate of collecting is promptly obtained the steroid compound product;
5) in step 2) in add deionized water in the residue after extracting; The mass ratio of said Inonqqus obliquus residue and deionized water is 1: 10～1: 30; Extracted 1～2 hour 90 ℃～100 ℃ temperature refluxed then; Lixiviate is 3 times repeatedly, and filtration under diminished pressure afterwards merges filtrating and obtain containing the Inonqqus obliquus test liquid of Fuscoporia obliqua polysaccharide and polyphenol;
6) under 40 ℃～60 ℃ temperature and the pressure of 0.06～0.1MPa, place Rotary Evaporators to be evaporated to 1/10～1/4 of original volume the above-mentioned Inonqqus obliquus test liquid that contains Fuscoporia obliqua polysaccharide and polyphenol; In concentrated solution, adding ethanol to ethanol mass concentration then is to precipitate more than 70%; Centrifugalize afterwards, collecting precipitation thing and supernatant respectively; Dehydrated alcohol with 2～3 times of volumes washs decolouring 3～5 times to above-mentioned precipitate then, and secondary precipitate is collected in centrifugalize afterwards;
7) secondary precipitate of collecting in the step 6) is drying to obtain the Fuscoporia obliqua polysaccharide product;
8) supernatant concentration that obtains in the step 6) is not distinguished the flavor of to there being alcohol, adopt the method that dynamically goes up appearance then, last kind to the good macroporous resin pillar of pretreatment; Water and mass concentration were that 30%～50% ethanol carries out gradient elution after last appearance was accomplished;
9) under 30 ℃～40 ℃ temperature and the pressure of 0.06～0.1MPa with step 8) in 30%～50% ethanol elution place the Rotary Evaporators concentrating under reduced pressure not have alcohol flavor, be drying to obtain Inonqqus obliquus polyphenol product then.
2. the method for from Inonqqus obliquus, extracting steroid compound, polysaccharide and polyphenol according to claim 1 is characterized in that: the pressure that described step 1) mesohigh is handled is 100KPA, and temperature is 121 ℃.
3. the method for from Inonqqus obliquus, extracting steroid compound, polysaccharide and polyphenol according to claim 1 is characterized in that: macroporous resin is D101 in the described step 8), and last appearance flow velocity is 2～3 times of column volume/h; The elution amount of each gradient is 3 times of column volume～4 times column volumes.
4. the method for from Inonqqus obliquus, extracting steroid compound, polysaccharide and polyphenol according to claim 1 is characterized in that: described step 4), 7) and 9) in the dry device that is adopted be freezer dryer, air drier or vacuum drier.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102898210A (en) * | 2012-09-20 | 2013-01-30 | 福建农林大学 | Medium for fermentation of Inonotus obliquus, and fermentation method for producing polysaccharide and application thereof |
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| CN104045728A (en) * | 2014-06-25 | 2014-09-17 | 陈文灵 | Method for refining inonotus obliquus (Fr.)Pilat crude polysaccharides by adopting resin D101 |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1398902A (en) * | 2002-05-21 | 2003-02-26 | 崔基成 | Extraction process of Fuscoporia obliqua polysaccharide |
| CN1723956A (en) * | 2004-07-23 | 2006-01-25 | 江苏省药用植物生物技术重点实验室 | Medicine for treating chronic gastritis and gastric carcinoma, and its prepn. method |
| CN101391958A (en) * | 2008-11-10 | 2009-03-25 | 马宏达 | Extraction method of medicinal fungus inonotus obliquus component |
| CN101392031A (en) * | 2008-11-10 | 2009-03-25 | 马宏达 | Extraction and separation method of inonotus obliquus polysaccharide |
| CN101422491A (en) * | 2008-11-11 | 2009-05-06 | 天津大学 | Preparation method of inonotus obilquus as alpha-glucosidase inhibitors |
| CN101731574A (en) * | 2008-11-11 | 2010-06-16 | 曲源 | Nano inonqqus obliquus |
| CN101849973A (en) * | 2010-06-11 | 2010-10-06 | 天津大学 | Total triterpenoid extract of inonotus obliquus with hypoglycemic function and pharmaceutical preparation thereof |
| CN102106875A (en) * | 2010-12-06 | 2011-06-29 | 浙江工业大学 | Method for extracting water-soluble alpha-glucosidase inhibitor |
-
2012
- 2012-03-13 CN CN2012100643327A patent/CN102579511B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1398902A (en) * | 2002-05-21 | 2003-02-26 | 崔基成 | Extraction process of Fuscoporia obliqua polysaccharide |
| CN1723956A (en) * | 2004-07-23 | 2006-01-25 | 江苏省药用植物生物技术重点实验室 | Medicine for treating chronic gastritis and gastric carcinoma, and its prepn. method |
| CN101391958A (en) * | 2008-11-10 | 2009-03-25 | 马宏达 | Extraction method of medicinal fungus inonotus obliquus component |
| CN101392031A (en) * | 2008-11-10 | 2009-03-25 | 马宏达 | Extraction and separation method of inonotus obliquus polysaccharide |
| CN101422491A (en) * | 2008-11-11 | 2009-05-06 | 天津大学 | Preparation method of inonotus obilquus as alpha-glucosidase inhibitors |
| CN101731574A (en) * | 2008-11-11 | 2010-06-16 | 曲源 | Nano inonqqus obliquus |
| CN101849973A (en) * | 2010-06-11 | 2010-10-06 | 天津大学 | Total triterpenoid extract of inonotus obliquus with hypoglycemic function and pharmaceutical preparation thereof |
| CN102106875A (en) * | 2010-12-06 | 2011-06-29 | 浙江工业大学 | Method for extracting water-soluble alpha-glucosidase inhibitor |
Non-Patent Citations (2)
| Title |
|---|
| 曾小龙: "桦褐孔菌的化学成分与药理作用研究", 《广东教育学院学报》 * |
| 项小燕等: "野生桦褐孔菌及其深层发酵产物的弱极性成分研究", 《中草药》 * |
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