CN101391958A - Extraction method of medicinal fungus inonotus obliquus component - Google Patents
Extraction method of medicinal fungus inonotus obliquus component Download PDFInfo
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- CN101391958A CN101391958A CNA2008101374891A CN200810137489A CN101391958A CN 101391958 A CN101391958 A CN 101391958A CN A2008101374891 A CNA2008101374891 A CN A2008101374891A CN 200810137489 A CN200810137489 A CN 200810137489A CN 101391958 A CN101391958 A CN 101391958A
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- obliquus
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- phaeopoms
- phaeopoms obliquus
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Abstract
The invention discloses a method for extracting and separating the efficacy element, fuscoporine, of medicinal fungi fuscoporia obliqua, which carries out desaltification processing to the fuscoporia obliqua by using a hydrochloric acid solution with the concentration of 0.5 to 2 percent, and then neutralizes the hydrochloric acid. Ethanol, sherwood oil, ethyl acetate, and normal butanol are respectively adopted to carry out step-by-step extraction separation. The extracting and separating method comprises the steps of desaltification processing to the salinity of the fuscoporia obliqua, step-by-step extraction separation by using the organic solvents of 30 to 50 percent of the sherwood oil, 33 to 65 percent of the ethyl acetate and 65 to 90 percent of the normal butanol after extracting the desalted fuscoporia obliqua by using 65 to 95 percent of ethanol solution, and finally obtaining of the fuscoporine with higher purity.
Description
Technical field
The present invention relates to a kind of extraction and separation method of functional component inonotus obliquus of medicinal fungi Phaeopoms obliquus, adopting ethanol, sherwood oil, vinyl acetic monomer, propyl carbinol after adopting hydrochloric acid to raw material soaking ammoniacal liquor neutral mode desalting treatment especially is the organic extraction solvent, extracts step by step through gradient and obtains the higher inonotus obliquus of purity.
Background technology
Phaeopoms obliquus [Inonotus obliguus (Fr) Pilat] is that a kind of application will be used fungi very widely on Russia, Poland, Finland and other places, English name chaga.Nineteen fifty-five, chaga is used for treating tumour, particularly Digestive tract and lung tumors by Russian Medicine Research Inst..16th century, the among the people of some countries of Eastern Europe prevented curing cancers with regard to the sclerotium with this wild fungus bacterium so far.This fungi of Siberian Khanty human prevents and treats heart trouble, hepatopathy, stomach trouble and esophagus disease etc.Recent research shows, Phaeopoms obliquus can suppress hiv virus (HIV-1), radioprotective and by the biosynthesizing of arrestin matter, antimitotic and eliminate that mechanism of action such as free radical activity suppresses or the growth and the control 0-157 intestinal bacteria that delay tumour cell poison.
The use of Phaeopoms obliquus is mainly based on wild, wild Phaeopoms obliquus mainly be grown in area, Muscovite siberian, Hokkaido, Japan, Finland, etc. ground.There is wild Phaeopoms obliquus to grow in China Changbai mountain, Jilin area and Zhang Guangcai mountain range, Heilungkiang and area, large and small Xing'an Mountains.
Biological study shows that Phaeopoms obliquus is grown very slow under wild state, wild kind is only grown on the birch that lives and just had very high pharmaceutical use in 10～15 years, and therefore wild Phaeopoms obliquus price is very expensive.The U.S. also classifies it as " special natural goods " following drink as the extraterrestrial.Therefore, the research of Phaeopoms obliquus and Products Development have very wide application prospect.It is the exclusive composition inonotus obliquus of Phaeopoms obliquus at the functional component that improves immunizing power, aspect effect such as anticancer that research report Phaeopoms obliquus is arranged.
Inonotus obliquus is that the peculiar composition of Phaeopoms obliquus is a kind of new compound that (2001) such as He Jian are found first and named.At present inonotus obliquus is extracted the extraction that only limits to research property in the laboratory and fail to realize industrialization extraction production, the present invention adopts organic solvent to extract step by step, the extraction back is reclaimed the method for organic solvent and extract inonotus obliquus from Phaeopoms obliquus, the extraction yield height of inonotus obliquus, organic solvent reclaims repeatedly and uses, and does not have energy consumption to be suitable for the suitability for industrialized production inonotus obliquus in the extraction process.
Summary of the invention
After the objective of the invention is to adopt Phaeopoms obliquus after the salt acid soak is pulverized to carry out desalting treatment, make raw material keep neutral with the hydrochloric acid soln that adds in the use ammonia soln, with an organic solvent ethanol, sherwood oil, vinyl acetic monomer, propyl carbinol carry out the higher inonotus obliquus of stage-by-stage extraction separation acquisition purity with the Phaeopoms obliquus after the desalting treatment.
Extraction and separation method of the present invention is to realize like this, use 0.5～3% hydrochloric acid soln of 1～5 times of weight of Phaeopoms obliquus weight to soak Phaeopoms obliquus after pulverizing, using after the salt acid soak in the ammoniacal liquor makes it keep neutral with the hydrochloric acid that adds, ethanol, 30～50% sherwood oil, 33～65% the vinyl acetic monomer, 65～90% the propyl carbinol that add concentration 65～99% in the solution after neutralization respectively carry out stage-by-stage extraction to be separated, and obtains the higher inonotus obliquus of purity.Concrete extraction separation process is as follows:
1. the hydrochloric acid soln that adds its 1～5 times of weight 0.5～2% in pulverizing the Phaeopoms obliquus of crossing 40～80 mesh sieves in the back soaks 10～24h and sloughs a large amount of salinities that contained in the natural Phaeopoms obliquus, to the hydrochloric acid that wherein adds in the ammonia soln and added, make Phaeopoms obliquus keep neutral after the desalting treatment.
2. the concentration that adds 3～8 times of amounts in the Phaeopoms obliquus that 1 obtains is ethanolic soln extraction 4～8h of 65～99%, abandons filter residue after the filtration, and pressurization is reclaimed the ethanol that adds and obtained solid extract.
3. 2 solid extracts that obtain are dissolved in 3～8 times the water, fully stir solid extract is suspended in the water, make suspension liquid.
4. after in 3, adding 30～50% petroleum ether solvent extraction solution layerings of 3～5 times of amounts, separate water layer and reclaim sherwood oil.
5. add 33～65% vinyl acetic monomer solvent extraction of 3～5 times of amounts in the water layers that 4 obtain, separate water layer, vinyl acetic monomer reclaims.
6. 65～90% butanol solutions extraction 3～5 times that adds 3～5 times of amounts in 5 water layers separates after the solution layering and gets n-butanol layer and get the solid white crystallisate and be inonotus obliquus.
Of the present invention having a few
Inonotus obliquus extracting method of the present invention, adopt organic solvent to extract step by step, the extraction back is reclaimed the method for organic solvent and extract inonotus obliquus from Phaeopoms obliquus, the extraction yield height of inonotus obliquus, organic solvent reclaims repeatedly and uses, and does not have energy consumption to be suitable for the suitability for industrialized production inonotus obliquus in the extraction process.
Claims (2)
1. an extracting method that is used for medicinal fungi Phaeopoms obliquus functional component inonotus obliquus is characterized in that the hydrochloric acid soln of working concentration 0.2～2% carries out the hydrochloric acid soln that the desalting treatment post neutralization adds to the Phaeopoms obliquus raw material.Ethanol, 30～50% sherwood oil, 33～65% vinyl acetic monomer, 65～90% the propyl carbinol of working concentration 65～99% carry out stage-by-stage extraction and separate respectively to the Phaeopoms obliquus after the desalting treatment.
2. according to the requirement of claim 1, the concrete extracting method of inonotus obliquus is as follows:
1) the hydrochloric acid soln immersion 10～24h that adds its 1～5 times of weight 0.5～2% in pulverizing the Phaeopoms obliquus of crossing 40～80 mesh sieves in the back sloughs a large amount of salinities that contained in the natural Phaeopoms obliquus, to the hydrochloric acid that wherein adds in the ammonia soln and added, make Phaeopoms obliquus keep neutral after the desalting treatment.
2) concentration that adds 3～8 times of amounts in the Phaeopoms obliquus that 1 obtains is that 65～99% ethanolic soln extracts 4～8h, abandons filter residue after the filtration, and pressurization is reclaimed the ethanol that adds and obtained solid extract.
3) 2 solid extracts that obtain are dissolved in 3～8 times the water, fully stir solid extract is suspended in the water, make suspension liquid.
4) in 3, add 30～50% petroleum ether solvent extraction solution layerings of 3～5 times of amounts after, separate water layer and reclaim sherwood oil.
5) add 33～65% vinyl acetic monomer solvent extraction of 3～5 times of amounts in the water layers that 4 obtain, separate water layer, vinyl acetic monomer reclaims.
6) in 5 water layers, add 65～90% butanol solutions extraction 3～5 times of 3～5 times of amounts, separate after the solution layering and get n-butanol layer and get the solid white crystallisate and be inonotus obliquus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101374891A CN101391958A (en) | 2008-11-10 | 2008-11-10 | Extraction method of medicinal fungus inonotus obliquus component |
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| CNA2008101374891A CN101391958A (en) | 2008-11-10 | 2008-11-10 | Extraction method of medicinal fungus inonotus obliquus component |
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| CN101391958A true CN101391958A (en) | 2009-03-25 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102000120A (en) * | 2010-11-17 | 2011-04-06 | 河北师范大学 | Method for extracting medicinal active substance from suillus luteus and application |
| CN102038720A (en) * | 2010-12-17 | 2011-05-04 | 中国农业大学 | Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients |
| CN102579511A (en) * | 2012-03-13 | 2012-07-18 | 天津大学 | Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus |
| CN104974125A (en) * | 2014-04-03 | 2015-10-14 | 中国医学科学院药物研究所 | Phaeoporlactone as well as preparation method, medicine composition and application thereof |
| CN106309509A (en) * | 2016-08-19 | 2017-01-11 | 广州滋得洛夫生物科技有限责任公司 | Inonotus obliquus polyphenol complex and preparation method thereof |
| CN107028930A (en) * | 2017-04-27 | 2017-08-11 | 牡丹江医学院 | A kind of antiprostate cancer composition and application thereof |
-
2008
- 2008-11-10 CN CNA2008101374891A patent/CN101391958A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102000120A (en) * | 2010-11-17 | 2011-04-06 | 河北师范大学 | Method for extracting medicinal active substance from suillus luteus and application |
| CN102000120B (en) * | 2010-11-17 | 2012-10-24 | 河北师范大学 | Method for extracting medicinal active substance from suillus luteus and application |
| CN102038720A (en) * | 2010-12-17 | 2011-05-04 | 中国农业大学 | Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients |
| CN102038720B (en) * | 2010-12-17 | 2012-05-02 | 中国农业大学 | Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients |
| CN102579511A (en) * | 2012-03-13 | 2012-07-18 | 天津大学 | Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus |
| CN102579511B (en) * | 2012-03-13 | 2013-11-27 | 天津大学 | Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus |
| CN104974125A (en) * | 2014-04-03 | 2015-10-14 | 中国医学科学院药物研究所 | Phaeoporlactone as well as preparation method, medicine composition and application thereof |
| CN104974125B (en) * | 2014-04-03 | 2019-01-01 | 中国医学科学院药物研究所 | Brown pore fungi lactone, its preparation method and its pharmaceutical composition and application |
| CN106309509A (en) * | 2016-08-19 | 2017-01-11 | 广州滋得洛夫生物科技有限责任公司 | Inonotus obliquus polyphenol complex and preparation method thereof |
| CN106309509B (en) * | 2016-08-19 | 2019-05-31 | 广州滋得洛夫生物科技有限责任公司 | Inonotus obliquus polyphenol complex compound and preparation method thereof |
| CN107028930A (en) * | 2017-04-27 | 2017-08-11 | 牡丹江医学院 | A kind of antiprostate cancer composition and application thereof |
| CN107028930B (en) * | 2017-04-27 | 2019-10-29 | 牡丹江医学院 | A kind of antiprostate cancer composition and application thereof |
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Open date: 20090325 |