CN102579511B - Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus - Google Patents
Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus Download PDFInfo
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Abstract
The invention relates to a method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus. The method mainly comprises the following steps: high-pressure treatment, ethanol extraction, concentration, centrifugation, deionized water extraction, concentration, ethanol precipitation, separation, purification, freezing and drying. Compared with the conventional extraction method, the provided method has the biggest difference in that high-pressure treatment is carried out to the raw material for preliminary treatment under the appropriate time condition, then an extract containing steroids is obtained by an ethanol extraction method, the steroidal compounds are purified by a water washing method and a centrifugation method, extracts of polysaccharides and polyphenols are obtained by a water extraction method, and then the polysaccharides and polyphenols are separated through ethanol precipitation. The tissue structure of inonotus obliquus changes due to high-pressure treatment, therefore, the yield of the steroidal compounds, polysaccharides and polyphenols is high, the molecular structure of active components in inonotus obliquus changes under high pressure, and the study verifies that the activity of inonotus obliquus can be enhanced.
Description
Technical field
The present invention relates to the extracting method of a kind of steroid compound, polysaccharide and polyphenol, particularly relate to a kind of from the comprehensive method of extracting steroid compound, polysaccharide and polyphenol Phaeopoms obliquus.
Background technology
Phaeopoms obliquus belongs to Mycophyta, Basidiomycotina, and Hymenomycetes, non-brown Zoopagales, Hymenochaetaceae, brown transverse hole fungus belongs to, formal name used at school Phaeoporousobliquus (per:Fr.) J.Schroet.The chemical constitution of Phaeopoms obliquus mainly contains vanillic acid, syringic acid and the gamma-hydroxy formic acid etc. of lanolin alkane type steroid compound, polyose, Polyphenols, lignin, melanochrome, alkaloids, tannin compound, folic acid derivatives and fragrance at present.At present, relevant researchist has launched research to the biological activity of these compositions, and is applied in the prevention and treatment of malignant tumour, diabetes, cardiovascular disorder, hepatopathy and acquired immune deficiency syndrome (AIDS).
The steroid material is a kind of main activeconstituents in Phaeopoms obliquus, and its content is large, active high, and Phaeopoms obliquus alcohol is a kind of main compound wherein, and research finds that it has good anti-tumor activity, is therefore paid close attention in recent years widely.
Fuscoporia obliqua polysaccharide is another the important active skull cap components in Phaeopoms obliquus, has different physiological roles and nontoxic to human body, is therefore also paid close attention to widely in recent years.Research finds that Fuscoporia obliqua polysaccharide has good anti-oxidant, hypoglycemic, antitumor and immunoregulation effect, and especially significant blood sugar decreasing effect and immunocompetence make it be expected to become the natural drug of prevent diabetes and cardiovascular disorder, strengthening immunity.
The Phaeopoms obliquus polyphenol is the natural product from extracting Phaeopoms obliquus, it due to the Phaeopoms obliquus polyphenol, is a kind of natural anti-oxidation preservation agent of high-efficiency low-toxicity, thereby in foodstuffs industry, be mainly used in the fresh-keeping of grease and goods thereof, fried and bake, fish and meat product, and can replace synthetic antioxidant BHA, BHT commonly used in present foodstuffs industry.In addition, that the Phaeopoms obliquus polyphenol has aspect medical is antiviral, antibiotic, antibacterial, prevention of dental caries, improve renal function, reducing blood-fat, the effect such as hypotensive, hypoglycemic, anti-oxidant, anticancer, and clinical study finds that the Phaeopoms obliquus polyphenol has good action to alleviating the cardiovascular systems pathology.
Adopt at present usually water extraction ethanol precipitation or solvent-extracted method carry out the extraction of polysaccharide and polyphenol in Phaeopoms obliquus and separate, because conventional water extraction ethanol precipitation extracting method yield is lower, the Fuscoporia obliqua polysaccharide yield only has 2%～5%, Phaeopoms obliquus polyphenol yield is lower, and the solvent extraction rule can cause environmental pollution because having used a large amount of organic solvents.And up to the present there is not yet a kind of report and example that can extract simultaneously the method for steroid compound, polysaccharide and polyphenol in Phaeopoms obliquus.
Summary of the invention
In order to address the above problem, the object of the present invention is to provide a kind of method from comprehensive extraction steroid compound, polysaccharide and polyphenol Phaeopoms obliquus that product yield is high.
In order to achieve the above object, provided by the inventionly from the comprehensive method of extracting steroid compound, polysaccharide and polyphenol Phaeopoms obliquus, comprise the following step carried out in order:
1) Phaeopoms obliquus is ground into to powder in electric mill, then at the high-pressure sterilizing pot mesohigh, processed 1～3 hour, so that raw material is carried out to pre-treatment;
2) above-mentioned Phaeopoms obliquus through autoclaving is joined in the ethanol of 80% concentration, the mass ratio of described Phaeopoms obliquus and ethanol is 1: 10～1: 20, then refluxing extraction 1～2 hour at the temperature of 80 ℃～90 ℃, lixiviate is 3 times repeatedly, filtration under diminished pressure afterwards, merge filtrate and obtain containing the Phaeopoms obliquus test liquid of steroid compound;
3) the above-mentioned Phaeopoms obliquus test liquid that contains steroid compound is placed in to the Rotary Evaporators vacuum rotary steam under the 40 ℃～temperature of 60 ℃ and the pressure of 0.06～0.1MPa extremely without ethanol, it with volume, is the water suspendible washing of above-mentioned concentrated rear 5～10 times of amounts of Phaeopoms obliquus test liquid, centrifugation afterwards, the collecting precipitation thing;
4) the above-mentioned drying precipitate of collecting is namely obtained to the steroid compound product;
5) in step 2) in add deionized water in residue after extracting, the mass ratio of described Phaeopoms obliquus residue and deionized water is 1: 10～1: 30, then refluxing extraction 1～2 hour at the temperature of 90 ℃～100 ℃, lixiviate is 3 times repeatedly, filtration under diminished pressure afterwards, merge filtrate and obtain containing the Phaeopoms obliquus test liquid of Fuscoporia obliqua polysaccharide and polyphenol;
6) under the 40 ℃～temperature of 60 ℃ and the pressure of 0.06～0.1MPa, the above-mentioned Phaeopoms obliquus test liquid that contains Fuscoporia obliqua polysaccharide and polyphenol is placed in to Rotary Evaporators and is evaporated to 1/10～1/4 of original volume, then in concentrated solution, add ethanol to ethanol mass concentration to precipitate more than 70%, centrifugation afterwards, collecting precipitation thing and supernatant liquor respectively; Then with the dehydrated alcohol of 2～3 times of volumes, above-mentioned throw out is washed to decolouring 3～5 times, centrifugation afterwards, collect secondary precipitate;
7) by step 6) in the secondary precipitate collected be drying to obtain the Fuscoporia obliqua polysaccharide product;
8) by step 6) in the supernatant concentration that obtains to without the alcohol flavor, then adopt the method for dynamic loading, be loaded on the macroporous resin pillar that pre-treatment is good; After completion of the sample, water and 30%～50% mass concentration ethanol carry out gradient elution;
9) under the 30 ℃～temperature of 40 ℃ and the pressure of 0.06～0.1MPa by step 8) in 30%～50% mass concentration ethanol eluate be placed in the Rotary Evaporators concentrating under reduced pressure and distinguish the flavor of without alcohol, then be drying to obtain Phaeopoms obliquus polyphenol product.
Described step 1) pressure of mesohigh processing is 100KPA, and temperature is 121 ℃.
Described step 8) in, macroporous resin is D101, and the loading flow velocity is 2～3 times of column volume/h; The elution amount of each gradient is 3 times of column volume～4 times column volumes.
Described step 4), 7) and 9) in the dry device adopted be freeze drier, hot air dryer or Vacuumdrier.
Maximum from the method for extracting steroid compound, polysaccharide and polyphenol Phaeopoms obliquus and existing extracting method difference provided by the invention is that the present invention first adopts high-pressure sterilizing pot, under suitable time conditions, raw material is carried out to autoclaving, so that raw material is carried out to pre-treatment, then utilize ethanol extraction method to obtain the extract that contains steroid, wash with water again and centrifugal method purifying steroid compound, with water extraction, obtain the extract of polysaccharide and polyphenol, then alcohol precipitation separating polyose and polyphenol.Because autoclaving makes the Phaeopoms obliquus weave construction, change, thereby the yield of steroid compound, polysaccharide and polyphenol is high, and under the effect of high pressure, in Phaeopoms obliquus, the structure of activeconstituents molecule can change, and can make its increased activity.In addition, because Phaeopoms obliquus is to treasure resource, adopt present method can promote the comprehensive utilization of resource.In addition, present method also has the simple advantage of technique.
The accompanying drawing explanation
Fig. 1 is for utilizing method provided by the invention and existing extracting method from Phaeopoms obliquus, extracting steroid compound, polysaccharide and polyphenol yield histogram.
Fig. 2 utilizes method provided by the invention and existing extracting method to suppress mda (MDA) generative capacity experimental result histogram from steroid compound, polysaccharide and the polyphenol extracted Phaeopoms obliquus.
Fig. 3 utilizes method provided by the invention and existing steroid compound, polysaccharide and the polyphenol reducing power ability experimental result histogram of extracting method from extracting Phaeopoms obliquus.
Embodiment
Below in conjunction with the drawings and specific embodiments, be elaborated from Phaeopoms obliquus, extracting steroid compound, polysaccharide and polyphenol method to provided by the invention.
Embodiment 1:
1) Phaeopoms obliquus is ground into to powder in electric mill, (100KPA, 121 ℃) autoclaving 1 hour in high-pressure sterilizing pot then, to carry out pre-treatment to raw material;
2) above-mentioned Phaeopoms obliquus through autoclaving is joined in the ethanol of 80% mass concentration, the mass ratio of described Phaeopoms obliquus and ethanol is 1: 20, then refluxing extraction 2 hours at the temperature of 90 ℃, lixiviate is 3 times repeatedly, filtration under diminished pressure afterwards, merge filtrate and obtain containing the Phaeopoms obliquus test liquid of steroid compound;
3) the above-mentioned Phaeopoms obliquus test liquid that contains steroid compound is placed in to the Rotary Evaporators vacuum rotary steam under the pressure of the temperature of 60 ℃ and 0.1MPa to without ethanol, it with volume, is the water suspendible washing of above-mentioned concentrated rear 10 times of amounts of Phaeopoms obliquus test liquid, centrifugation afterwards, the collecting precipitation thing;
4) throw out of above-mentioned collection is carried out to drying with freeze drier, hot air dryer or Vacuumdrier and can obtain the steroid compound series products, its yield is 0.96%, and with Vanillin-ice acetic acid method, recording its steroidal content is 78.20%;
5) in step 2) in add deionized water in residue after extracting, the mass ratio of described Phaeopoms obliquus residue and deionized water is 1: 30, then refluxing extraction 2 hours at the temperature of 100 ℃, lixiviate is 3 times repeatedly, filtration under diminished pressure afterwards, merge filtrate and obtain containing the Phaeopoms obliquus test liquid of Fuscoporia obliqua polysaccharide and polyphenol;
6) under the pressure of the temperature of 60 ℃ and 0.1MPa, the above-mentioned Phaeopoms obliquus test liquid that contains Fuscoporia obliqua polysaccharide and polyphenol is placed in to Rotary Evaporators and is evaporated to 1/4 of original volume, then in concentrated solution, add ethanol to ethanol mass concentration to precipitate more than 70%, centrifugation afterwards, collecting precipitation thing and supernatant liquor respectively; Then with the dehydrated alcohol of 3 times of volumes, above-mentioned throw out is washed to decolouring 5 times, centrifugation afterwards, collect secondary precipitate;
7) by step 6) in the secondary precipitate collected with freeze drier, hot air dryer or Vacuumdrier, carry out drying and can obtain the Fuscoporia obliqua polysaccharide product, its yield is 7.69%, with sulfuric acid-phynol method, recording its polysaccharide content is 43.73%;
8) by step 6) in the supernatant concentration that obtains to without the alcohol flavor, then adopt the method for dynamic loading, be loaded on the D101 macroporous resin pillar that pre-treatment is good, the loading flow velocity is 3 times of column volume/h; After completion of the sample, water and 30% ethanol carry out wash-out, 4 times of column volumes of each gradient elution;
9) under the pressure of the temperature of 40 ℃ and 0.1MPa by step 8) in 30% mass concentration ethanol eluate be placed in the Rotary Evaporators concentrating under reduced pressure and distinguish the flavor of without alcohol, then by dryings such as freeze drier, hot air dryer or Vacuumdriers, can obtain Phaeopoms obliquus polyphenol product.Content through Folin-Ciocalteu colorimetric method for determining polyphenol is 25.40%, and yield is 1.65%.
Embodiment 2:
1) by Phaeopoms obliquus grinding powder in electric mill, (100KPA, 121 ℃) autoclaving 2 hours in high-pressure sterilizing pot then, to carry out pre-treatment to raw material;
2) above-mentioned Phaeopoms obliquus through autoclaving is joined in the ethanol of 80% mass concentration, the mass ratio of described Phaeopoms obliquus and ethanol is 1: 15, then refluxing extraction 1.5 hours at the temperature of 80 ℃, lixiviate is 3 times repeatedly, filtration under diminished pressure afterwards, merge filtrate and obtain containing the Phaeopoms obliquus test liquid of steroid compound;
3) the above-mentioned Phaeopoms obliquus test liquid that contains steroid compound is placed in to the Rotary Evaporators vacuum rotary steam under the pressure of the temperature of 50 ℃ and 0.08MPa to without ethanol, it with volume, is the water suspendible washing of above-mentioned concentrated rear 7 times of amounts of Phaeopoms obliquus test liquid, centrifugation afterwards, the collecting precipitation thing;
4) throw out of above-mentioned collection is carried out to drying with freeze drier, hot air dryer or Vacuumdrier and can obtain the steroid compound series products, its yield is 1.06%, and with Vanillin-ice acetic acid method, recording its steroidal content is 83.97%;
5) in step 2) in add deionized water in residue after extracting, the mass ratio of described Phaeopoms obliquus residue and deionized water is 1: 20, then refluxing extraction 1.5 hours at the temperature of 80 ℃, lixiviate is 3 times repeatedly, filtration under diminished pressure afterwards, merge filtrate and obtain containing the Phaeopoms obliquus test liquid of Fuscoporia obliqua polysaccharide and polyphenol;
6) under the pressure of the temperature of 50 ℃ and 0.08MPa, the above-mentioned Phaeopoms obliquus test liquid that contains Fuscoporia obliqua polysaccharide and polyphenol is placed in to Rotary Evaporators and is evaporated to 1/8 of original volume, then in concentrated solution, add ethanol to ethanol mass concentration to precipitate more than 70%, centrifugation afterwards, collecting precipitation thing and supernatant liquor respectively; Then with the dehydrated alcohol of 2.5 times of volumes, above-mentioned throw out is washed to decolouring 4 times, centrifugation afterwards, collect secondary precipitate;
7) by step 6) in the secondary precipitate collected with freeze drier, hot air dryer or Vacuumdrier, carry out drying and can obtain the Fuscoporia obliqua polysaccharide product, its yield is 9.00%, with sulfuric acid-phynol method, recording its polysaccharide content is 43.59%;
8) by step 6) in the supernatant concentration that obtains to without the alcohol flavor, then adopt the method for dynamic loading, be loaded on the D101 macroporous resin pillar that pre-treatment is good, the loading flow velocity is 2.5 times of column volume/h; After completion of the sample, water and 50% mass concentration ethanol carry out wash-out, 3.5 times of column volumes of each gradient elution;
9) under the pressure of the temperature of 35 ℃ and 0.08MPa by step 8) in 50% mass concentration ethanol eluate be placed in the Rotary Evaporators concentrating under reduced pressure and distinguish the flavor of without alcohol, then with freeze drier, hot air dryer or Vacuumdrier, carry out drying and can obtain Phaeopoms obliquus polyphenol product.Content through Folin-Ciocalteu colorimetric method for determining polyphenol is 20.27%, and yield is 1.22%.
Embodiment 3:
1) by Phaeopoms obliquus grinding powder in electric mill, (100KPA, 121 ℃) autoclaving 3h in high-pressure sterilizing pot then, to carry out pre-treatment to raw material;
2) above-mentioned Phaeopoms obliquus through autoclaving is joined in the ethanol of 80% mass concentration, the mass ratio of described Phaeopoms obliquus and ethanol is 1: 10, then refluxing extraction 1 hour at the temperature of 80 ℃, lixiviate is 3 times repeatedly, filtration under diminished pressure afterwards, merge filtrate and obtain containing the Phaeopoms obliquus test liquid of steroid compound;
3) the above-mentioned Phaeopoms obliquus test liquid that contains steroid compound is placed in to the Rotary Evaporators vacuum rotary steam under the pressure of the temperature of 40 ℃ and 0.06MPa to without ethanol, it with volume, is the water suspendible washing of above-mentioned concentrated rear 5 times of amounts of Phaeopoms obliquus test liquid, centrifugation afterwards, the collecting precipitation thing;
4) throw out of above-mentioned collection is carried out to drying with freeze drier, hot air dryer or Vacuumdrier and can obtain the steroid compound series products, its yield is 0.82%, and with Vanillin-ice acetic acid method, recording its steroidal content is 81.48%;
5) in step 2) in add deionized water in residue after extracting, the mass ratio of described Phaeopoms obliquus residue and deionized water is 1: 10, then refluxing extraction 1 hour at the temperature of 90 ℃, lixiviate is 3 times repeatedly, filtration under diminished pressure afterwards, merge filtrate and obtain containing the Phaeopoms obliquus test liquid of Fuscoporia obliqua polysaccharide and polyphenol;
6) under the pressure of the temperature of 40 ℃ and 0.06MPa, the above-mentioned Phaeopoms obliquus test liquid that contains Fuscoporia obliqua polysaccharide and polyphenol is placed in to Rotary Evaporators and is evaporated to 1/10 of original volume, then in concentrated solution, add ethanol to ethanol mass concentration to precipitate more than 70%, centrifugation afterwards, collecting precipitation thing and supernatant liquor respectively; Then with the dehydrated alcohol of 3 times of volumes, above-mentioned throw out is washed to decolouring 3 times, centrifugation afterwards, collect secondary precipitate;
7) by step 6) in the secondary precipitate collected with freeze drier, hot air dryer or Vacuumdrier, carry out drying and can obtain the Fuscoporia obliqua polysaccharide product, its yield is 8.03%, with sulfuric acid-phynol method, recording its polysaccharide content is 42.68%;
8) by step 6) in the supernatant concentration that obtains to without the alcohol flavor, then adopt the method for dynamic loading, be loaded on the D101 macroporous resin pillar that pre-treatment is good, the loading flow velocity is 2 times of column volume/h; After completion of the sample, water and 40% mass concentration ethanol carry out wash-out, 3 times of column volumes of each gradient elution;
9) under the pressure of the temperature of 30 ℃ and 0.06MPa by step 8) in 40% mass concentration ethanol eluate be placed in the Rotary Evaporators concentrating under reduced pressure and distinguish the flavor of without alcohol, then with freeze drier, hot air dryer or Vacuumdrier, carry out drying and can obtain Phaeopoms obliquus polyphenol product.Content through Folin-Ciocalteu colorimetric method for determining polyphenol is 22.85%, and yield is 1.24%.
The inventor will utilize the method that the invention described above provides to reach existing extracting method from Phaeopoms obliquus, extracting the yield drafting pattern of Phaeopoms obliquus steroid compound, polysaccharide and polyphenol, the results are shown in Figure 1.
In addition, the inventor to utilize that above-described embodiment provides Phaeopoms obliquus steroid compound, polysaccharide and polyphenol that Phaeopoms obliquus steroid compound, polysaccharide and polyphenol method obtain suppress mda (MDA) generative capacity and the reducing power ability is tested from Phaeopoms obliquus, extracting.
It is reported, lipid peroxidation can cause very large damage to cell, and it is a kind of main peroxy radical, MDA be cause human senility, cause a disease, one of carcinogenic important factor.Therefore, the content of measuring MDA not only can reflect the snperoxiaized degree of cytolipin, and can reflect the degree of damage of MDA to cell.
Obviously between the reducing power of sample and a resistance of oxidation, direct relation is arranged.The mechanism of the reducing power of sample comes from the chain that interrupts free radical, thereby or reacts with the precursor of free radical the formation that stops peroxy.
Phaeopoms obliquus steroid compound, polysaccharide and the polyphenol that inhibition mda (MDA) generative capacity and the experiment of reducing power ability are used is all to adopt the method for autoclaving condition to obtain, and contrasts with Phaeopoms obliquus steroid compound, polysaccharide and polyphenol that untreated existing method obtains.
Suppress mda (MDA) generative capacity experimental result and see Fig. 2.Result shows, with Phaeopoms obliquus steroid compound, polysaccharide and polyphenol that untreated existing method obtains, compare, utilize Phaeopoms obliquus steroid compound, polysaccharide and the polyphenol that the inventive method obtains all to obtain enhancing in various degree to the inhibition ability that mouse MDA generates.
Reducing power ability experimental result is shown in Fig. 3.Result shows, compares with Phaeopoms obliquus steroid compound, polysaccharide and polyphenol that untreated existing method obtains, utilizes Phaeopoms obliquus steroid compound, polysaccharide and the polyphenol reducing power ability that the inventive method obtains all to obtain enhancing in various degree.
Claims (3)
1. one kind from the comprehensive method of extracting steroid compound, polysaccharide and polyphenol Phaeopoms obliquus, it is characterized in that: describedly from the method for extracting steroid compound, polysaccharide and polyphenol Phaeopoms obliquus, comprise the following step carried out in order:
1) Phaeopoms obliquus is ground into to powder in electric mill, then at the high-pressure sterilizing pot mesohigh, processed 1～3 hour, so that raw material is carried out to pre-treatment; The pressure of autoclaving is 100KPA, and temperature is 121 ℃;
2) above-mentioned Phaeopoms obliquus through autoclaving is joined in the ethanol of 80% mass concentration; the mass ratio of described Phaeopoms obliquus and ethanol is 1: 10～1: 20; then refluxing extraction 1～2 hour at the temperature of 80 ℃～90 ℃; lixiviate is 3 times repeatedly; filtration under diminished pressure afterwards, merge filtrate and obtain containing the Phaeopoms obliquus test liquid of steroid compound;
3) the above-mentioned Phaeopoms obliquus test liquid that contains steroid compound is placed in to the Rotary Evaporators vacuum rotary steam under the 40 ℃～temperature of 60 ℃ and the pressure of 0.06～0.1MPa extremely without ethanol, it with volume, is the water suspendible washing of above-mentioned concentrated rear 5～10 times of volumes of Phaeopoms obliquus test liquid, centrifugation afterwards, the collecting precipitation thing;
4) the above-mentioned drying precipitate of collecting is namely obtained to the steroid compound product;
5) in step 2) in add deionized water in residue after extracting, the mass ratio of described Phaeopoms obliquus residue and deionized water is 1: 10～1: 30, then refluxing extraction 1～2 hour at the temperature of 90 ℃～100 ℃, lixiviate is 3 times repeatedly, filtration under diminished pressure afterwards, merge filtrate and obtain containing the Phaeopoms obliquus test liquid of Fuscoporia obliqua polysaccharide and polyphenol;
6) under the 40 ℃～temperature of 60 ℃ and the pressure of 0.06～0.1MPa, the above-mentioned Phaeopoms obliquus test liquid that contains Fuscoporia obliqua polysaccharide and polyphenol is placed in to Rotary Evaporators and is evaporated to 1/10～1/4 of original volume, then in concentrated solution, adding ethanol to ethanol mass concentration is to precipitate more than 70%, centrifugation afterwards, collecting precipitation thing and supernatant liquor respectively; Then with the dehydrated alcohol of 2～3 times of volumes, above-mentioned throw out is washed to decolouring 3～5 times, centrifugation afterwards, collect secondary precipitate;
7) secondary precipitate of collecting in step 6) is drying to obtain to the Fuscoporia obliqua polysaccharide product;
8) supernatant concentration to the nothing alcohol obtained in step 6) is distinguished the flavor of, then adopt the method for dynamic loading, be loaded on the macroporous resin pillar that pre-treatment is good; After completion of the sample, water and mass concentration are that 30%～50% ethanol carries out gradient elution;
9) under the 30 ℃～temperature of 40 ℃ and the pressure of 0.06～0.1MPa, 30%～50% ethanol eluate in step 8) is placed in to the Rotary Evaporators concentrating under reduced pressure and distinguishes the flavor of without alcohol, then be drying to obtain Phaeopoms obliquus polyphenol product.
2. according to claim 1 from Phaeopoms obliquus, extracting the method for steroid compound, polysaccharide and polyphenol, it is characterized in that: in described step 8), macroporous resin is D101, and the loading flow velocity is 2～3 times of column volume/h; The elution amount of each gradient is 3 times of column volume～4 times column volumes.
3. according to claim 1 from Phaeopoms obliquus, extracting the method for steroid compound, polysaccharide and polyphenol, it is characterized in that: described step 4), 7) and 9) in the dry device adopted be freeze drier, hot air dryer or Vacuumdrier.
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