CN102898210B - Medium for fermentation of Inonotus obliquus, and fermentation method for producing polysaccharide and application thereof - Google Patents
Medium for fermentation of Inonotus obliquus, and fermentation method for producing polysaccharide and application thereof Download PDFInfo
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 74
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 72
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 72
- 238000000855 fermentation Methods 0.000 title claims abstract description 33
- 230000004151 fermentation Effects 0.000 title claims abstract description 33
- 241000414067 Inonotus obliquus Species 0.000 title abstract description 6
- 238000004519 manufacturing process Methods 0.000 title abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
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- 238000000034 method Methods 0.000 claims abstract description 17
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- 241000222336 Ganoderma Species 0.000 description 2
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- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
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Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a medium for fermentation of Inonotus obliquus, and a fermentation method for producing polysaccharide and application thereof. The medium comprises 15-25g / L corn flour, 5-15g / L glucose, 10-30g / L wheat bran, 3-7g / L yeast powder, 1g / L KH2PO4, 0.5g / L MgSO4.7H2O, 0.005g / L VB1 and 1L of water, and has a pH value of 5-7. The fermentation conditions are as below: rotation speed of 110-140R / min, temperature of 26-31 DEG C and fermentation time of 11-14 days. Crude polysaccharide is extracted; polysaccharide content is determined; and finally animal test shows that the polysaccharide has effects of significantly enhancing animal endurance, delaying fatigue and accelerating fatigue. The invention can substantially increase polysaccharide content and yield of Inonotus obliquus, and has economical and practical formula, and simple conditions and process.
Description
Technical field
The present invention is specifically related to method and the application of a kind of substratum for Phaeopoms obliquus fermentation and fermentation product polysaccharide.
Background technology
Phaeopoms obliquus (
inonotus obliquus) have another name called the fine pore fungi of Chaga, black birch bacterium, birch bacterium, inclined tube, its be born under the deciduous trees barks such as white birch, silvery birch, elm, alder or cut raft after on trees dried-up, form sterile wood-decay fungi, its sclerotium can be after felling dried-up on existence reach 6 years, cause the white rot of white birch, silvery birch, elm, alder etc.Phaeopoms obliquus is the wood-decay fungi that is grown in frigid zone, extremely cold-resistant, and the Phaeopoms obliquus mycelium in timber can not freezed to death at-40 ℃.Phaeopoms obliquus is mainly distributed in the cold district between 40 °-50 ° of north latitude such as Russian northern siberian, the Far East Area and Kamchatka Peninsula, Finland, Poland, Hokkaido, Japan, Hokkaido, Olynets, Baltics, the large Xiaoxinanlin Mountains, China Dark Longjiang, Changbai mountain, Jilin and northern North america, mainly concentrates on 45 ° of-50 ° of areas of north latitude.Cannot come strain identification sibship far and near by inonotus obliquus sclerotium profile.From l6, since century, Phaeopoms obliquus is praised as " glossy ganoderma in siberia ", in Eastern Europe, among the people being widely used such as North America, Russia, Poland, Finland, is used for preventing and treating various difficult and complicated cases, such as various cancers, heart trouble, diabetes etc.The northern people of Russia regards as it the present that God grants a kind of mystery of the suffering mankind always, and Japanology personnel claim that it is " panacea ", and the U.S. lists it in " special crude substance ", as extraterrestrial's following drink.
Phaeopoms obliquus not only can significantly suppress the growth of animal-transplanted tumor, also have strengthen immune, anti-oxidant, antiviral, anti-mutation, inhibition HIV infects and treat the multiple effects such as diabetes.
In recent years, the various medicinal fungus polysaccharide such as glossy ganoderma, mushroom been have all have been researched and developed into functional food, and Phaeopoms obliquus is also unwilling to be outshone naturally, have shown boundless prospect in clinical application.Many researchers lay stress on the prevent and treat aspect of Phaeopoms obliquus to cancer, and experiment effect is desirable.Therefore, Phaeopoms obliquus is just being applied to the assisting therapy of cancer clinically at present.In addition, Phaeopoms obliquus also has a great development in the development and application aspect other diseases and health care.For example, it is a kind of natural balance hypoglycemic drink of the various active composition relevant with treatment diabetes to prevention containing extracting in Phaeopoms obliquus that the Tunguses-birch reveals, can make diabetics avoid the side effect of traditional antidiabetic drug to internal organs grievous injuries such as liver kidneys, and obtain national inventing patent.The Phaeopoms obliquus fine powder that the production of Suo Molesiji (Komsomlski) drugmaker is born by Russia also reaches 93% height to the curative ratio of diabetes.Phaeopoms obliquus is also natural antioxidants desirable in foodstuffs industry, can in food applications, become the foodstuff additive that play preservative activity.Phaeopoms obliquus can also be as supplement and the food dye raw material of processing class meat, the Chinese medicinal materialss etc. such as beverage, seasonings, biscuit bread, sausage.It is added in food, can brings into play the effect of Phaeopoms obliquus to product, can bring into play again the effect of Phaeopoms obliquus to human body, kill two birds with one stone.
Fungus polysaccharide the reach of science allows 21 century become " fungus polysaccharide century ", and the polysaccharide of edible medicinal fungus has become the study hotspot in the world, is one of edible medicinal product that have most at present exploitation future.Phaeopoms obliquus is as a kind of rare famous and precious fungi, its most important figures in the expression of value of the linen, as an equivalent, and consequently, as a thing that is value now its medicinal on.Phaeopoms obliquus is excellent medicinal fungus, at antitumor, aspect such as treatment diabetes etc., has significant effect.Phaeopoms obliquus is widely used already for a long time the among the people of Russian Finland, but in China, the research starting evening of Phaeopoms obliquus, and Phaeopoms obliquus climate environmental influence is large, be not suitable in the higher place cultivation of temperature, the wild sporophore that occurring in nature forms is more rare, and in ratio, every 20000 strain birches just have a strain life, and growth cycle is long, it only grows on birch more than 10 years just good medicinal effect.And the artificial culture of China's Phaeopoms obliquus is only obtained limited success at present, poor repeatability, quality and Character instability, temporarily can't blindly carry out extensive artificial culture.Want to reach mass-producing or industrialization artificial culture, still also have very remote road to walk, cannot meet current human wants at all.But the research of Phaeopoms obliquus and exploitation vast potential for future development and the space of related products have been given also just because of this.Due to the sclerotium growth impact that waits the aspects such as environment consuming time, the acquisition of its sclerotium is restricted, therefore by liquid fermenting, obtain its mycelium, be the Main Means that obtains Fuscoporia obliqua polysaccharide.
summary of the invention
A kind of method that the object of the present invention is to provide substratum for Phaeopoms obliquus fermentation and fermentation to produce polysaccharide, the present invention can significantly improve the polysaccharide content of Phaeopoms obliquus, fill a prescription economical and practical, condition and simple for process, the effect of the polysaccharide of generation at delaying the emergence of fatigue and in accelerating elimination of fatigue.
First a kind of substratum for Phaeopoms obliquus fermentation is provided, and described culture medium prescription is: Semen Maydis powder 15-25g/L, glucose 5-15g/L, wheat bran 10-30g/L, yeast powder 3-7g/L, KH
2pO
41g/L, MgSO
4 .7H
2o 0.5g/L, V
b10.005g/L, water 1L, pH value is 5-7.
More preferably, described culture medium prescription is: Semen Maydis powder 20g/L, glucose 15g/L, wheat bran 30g/L, yeast powder 3g/L, KH
2pO
41g/L, MgSO
4 .7H
2o 0.5g/L, V
b10.005g/L, water 1L, pH value is 5-7.
A method of utilizing above-mentioned substratum fermentation to produce polysaccharide, is characterized in that: fermentation condition: rotating speed 110-140 r/min, temperature 26.0-31.0 ℃, fermentation 11-14d; Crude polysaccharides extraction step is as follows:
(1) in born of the same parents, Crude polysaccharides extracts: filtering fermentation liquor or centrifugal, collect mycelium, and dry, pulverize, take 1 g mycelium powder, add 10-30ml distilled water, heat 0.5-3h at 80-100 ℃, filter, filtrate rotary evaporation, be concentrated into 1/3 of original volume, then add 3 times of volume alcohol, standing 24h, centrifugal, collect flocks;
(2) crude extracellular polysaccharide extracts: the fermented liquid that filters mycelia is collected, be concentrated into 1/5 of original volume, add 3 times of volume alcohol, and standing, centrifugal, collect flocks;
Merge the outer flocks of born of the same parents in born of the same parents, freeze-drying, obtains Crude polysaccharides, water-soluble with phenol sulfuric acid process survey polysaccharide content.
More preferably, fermentation condition: rotating speed 140 r/min, 30 ℃ of temperature, fermentation 12d.
The present invention also provides the application of the Phaeopoms obliquus polysaccharide that fermentation produces in preparing anti-fatigue medicament.
The Phaeopoms obliquus adopting in the specific embodiment of the invention be Phaeopoms obliquus (
inonotus obliquus) MRC I0001, on July 12nd, 2012, be preserved in Chinese Typical Representative culture collection center (CCTCC), address: Wuhan Wuhan University, preserving number is CCTCC M 2012283.
Advantage of the present invention: the one, can significantly improve the inside and outside polysaccharide content of born of the same parents of Phaeopoms obliquus, through Optimal Medium formula, the steps such as optimization of fermentation conditions obtain the inside and outside polysaccharide content of higher born of the same parents; For the fermentation character of Phaeopoms obliquus, certain basis is established in the deep level development utilization of the chemical composition analysis of tunning and Phaeopoms obliquus series product research; For the production development of Phaeopoms obliquus liquid submerged fermentation and related products provides scientific and reasonable technique; For providing theoretical, the standardization fermentative production of Phaeopoms obliquus and processing quality monitoring use for reference and technical director, and the 2nd, by animal experiment, prove that the Fuscoporia obliqua polysaccharide extracting has the significant effect that strengthens animal endurance, delaying the emergence of fatigue and accelerate elimination of fatigue.
Accompanying drawing explanation
Fig. 1 is standard glucose graphic representation.
Embodiment
Be below extraction and the assay of the interior exo polysaccharides of born of the same parents in specific embodiment:
(1) in born of the same parents, crude extracellular polysaccharide extracts
In born of the same parents, Crude polysaccharides extracts: dry and collect after mycelium, pulverize, get 1g mycelium powder adding distil water 30ml, 100 ℃ of dryers boil 3 hours.Vacuum filtration, rotary evaporation, is concentrated into 1/3 of original volume afterwards.Add 3 times of analytical pure alcohol (raw spirit), standing 24h.Centrifugal (10000r/min, 4 ℃ of 10min), collect flocks.
Crude extracellular polysaccharide extracts: the fermented liquid that falls mycelia by filtered through gauze is collected, then used vacuum rotary evaporator rotary evaporation, be concentrated into 1/5 of original volume, add 3 times of analytical pure alcohol (raw spirit), standing 24h.Centrifugal (10000r/min, 4 ℃ of 10min), collect flocks.
Merge the outer flocks of born of the same parents in born of the same parents, freeze-drying 5h-6h, obtains Crude polysaccharides.
(2) determination of polysaccharide---phenolsulfuric acid method
1. the preparation of 0.1% glucose reference liquid
Accurately take 105 ℃ of standard glucose 50.00mg constant volumes that are dried to constant weight in 100ml volumetric flask, being mixed with concentration is the glucose standard mother liquor of 0.5000mg/ml; Get above-mentioned mother liquor 10ml constant volume in 50ml volumetric flask, being mixed with concentration is the glucose reference liquid of 0.1000mg/ml.
2. the preparation of 5% phenol solution
Accurately take 25.0005g re-distilled phenol, constant volume, in 500ml volumetric flask, is mixed with 5.0001% phenol solution.
3. glucose typical curve preparation
Draw glucose reference liquid 0.0,0.1,0.2,0.4,0.6,0.8,1.0,1.2,1.4ml is placed in respectively tool plug test tube, add water to respectively 2.0ml, respectively add phenol test solution 1.0 ml and shake up, drip rapidly the vitriol oil 5.0 ml, after vibration mixes, be placed in ice bath 20min, take out and be placed in room temperature, separately, with the same blank that is operating as of distilled water 2.0 ml, in 490nm place, measure light absorption value, draw glucose curve.Take dextrose standard sample concentration as X-coordinate, and absorbancy is ordinate zou, as shown in Figure 1: y=0.7012x-0.0063 (R
2=0.9995).
4. sample determination of polysaccharide
Take respectively Crude polysaccharides sample (in triplicate) constant volume of 5.0mg left and right above-mentioned steps (2) in 25ml volumetric flask, draw respectively polysaccharide sample liquid 1.0ml, by step, 3. operate.
Embodiment 1
By different nitrogenous sources (wheat bran 50g/L, analysis for soybean powder 50g/L, Semen Maydis powder 50g/L, yeast powder 50g/L, extractum carnis 2g/L, ammonium tartrate 0.32g/L, peptone 2g/L), carbon source (flour 20g/L, ground rice 20g/L, Semen Maydis powder 20g/L, glucose 20g/L, N.F,USP MANNITOL 20g/L, sucrose 20g/L), by single Factor Selection and orthogonal test (table 1), the optimal liquid fermentative medium formula that obtains being conducive to mycelial growth and obviously improve the inside and outside polysaccharide content of born of the same parents: Semen Maydis powder 20g/L, glucose 15g/L, wheat bran 30g/L, yeast powder 3g/L, KH
2pO
41g/L, MgSO
4 .7H
2o 0.5g/L, V
b10.005g/L(fermentation condition: be placed in 25 ℃, rotating speed is in the shaking table of 150r/min, shaking culture 10d), general formulation before optimizing: glucose 3%, peptone 0.3%, yeast powder 0.2%, ammonium sulfate 0.2%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, pH nature, is settled to 1000mL, and Crude polysaccharides content is only 0.253 g/100mL, and Crude polysaccharides amount reaches 0.373g/100mL after optimizing, polysaccharide amount improves 47.4% before than formulation optimization.
Table 1 culture medium prescription orthogonal test level of factor table
Specific experiment step is as follows:
1. the impact of different nitrogen sources on Phaeopoms obliquus Crude polysaccharides output
Substratum is sub-packed in (100mL/ bottle) in 250mL triangular flask, and 4 repetitions are established in each processing, sterilizing 20min at 121 ℃.After cooling, in substratum, add according to quantity the VITAMIN liquid through filter degerming.During inoculation, first slant strains is grilled in the triangular flask that fills sterilized water, with decollator, stirs homogenate, make it to become uniform bacterium liquid.Then with liquid-transfering gun, draw bacterium liquid and be inoculated in (1mL bacterium liquid/100mL) in each bottle of substratum, be placed in 25 ℃, random alignment in the shaking table that rotating speed is 150r/min, shaking culture 10d, covers with mycelium pellet, and solution turns orange taking-up when transparent.
2. the impact of different carbon sources on Phaeopoms obliquus Crude polysaccharides output
Substratum is sub-packed in (100mL/ bottle) in 250mL triangular flask, and 4 repetitions are established in each processing, sterilizing 20min at 121 ℃.After cooling, in substratum, add according to quantity the VITAMIN liquid through filter degerming.During inoculation, first slant strains is grilled in the triangular flask that fills sterilized water, with decollator, stirs homogenate, make it to become uniform bacterium liquid.Then with liquid-transfering gun, draw bacterium liquid and be inoculated in (1mL bacterium liquid/100mL) in each bottle of substratum, be placed in 25 ℃, random alignment in the shaking table that rotating speed is 150r/min, shaking culture 10d, covers with mycelium pellet, and solution turns orange taking-up when transparent.
3. orthogonal experiment
The Crude polysaccharides output inside and outside Phaeopoms obliquus born of the same parents of take is index, selects L9(3
4) orthogonal table, take Semen Maydis powder (A), glucose (B), wheat bran (C), yeast powder (D) carries out orthogonal experiment as direct factor, separately with KH
2pO
41g/L, MgSO
47H
2o 0.5g/L, V
b10.005g/L, water 1L is standard.And data are carried out to variance analysis, then the optimum combination filtering out is carried out to confirmatory experiment.
Embodiment 2
The optimal liquid fermention medium that adopts embodiment bis-to obtain, by different pH values, (pH 4, pH 5, pH 6, pH 7, pH 8, pH 9, pH 10), shaking speed (100 rpm, 120 rpm, 140 rpm, 160rpm, 180 rpm), (20 ℃ of temperature, 25 ℃, 28 ℃, 31 ℃, 36 ℃), plant (5d in age, 6d, 7d, 8d, 9d) and inoculum size (5%, 7.5%, 10%, 12.5%, 15%), fermentation number of days (10d, 11d, 12d, 13d, 14d), by single Factor Selection and orthogonal test (table 2), obtain being conducive to mycelial growth and obviously improve in born of the same parents, the optimal liquid fermentation condition of exo polysaccharides content: pH6, rotating speed 140 r/min, 30 ℃ of temperature, fermentation number of days 12d, after optimizing, Crude polysaccharides amount reaches 0.511g/100mL.
Table 2 fermentation condition orthogonal test level of factor table
Specific experiment step is as follows:
1, the impact of pH on Phaeopoms obliquus Crude polysaccharides output
With hydrochloric acid or sodium hydroxide solution, the pH of substratum is adjusted to respectively to 4,5,6,7,8,9,10.Respectively by cultured liquid seeds with after quick refiner homogenate according to volume fraction 10% inoculum size be inoculated in initial pH value be 4,5,6,7,8,9,10 and the natural substratum of pH in, be placed in 25 ℃, rotating speed is random alignment in the shaking table of 150r/min, and shaking culture 10d takes out.4 repetitions are established in each processing.
2, the impact of rotating speed on Phaeopoms obliquus Crude polysaccharides output
Respectively by cultured liquid seeds with being inoculated in substratum according to volume fraction 10% inoculum size after quick refiner homogenate, be placed in respectively 25 ℃, rotating speed is in the shaking table of 100r/min, 120r/min, 140r/min, 160r/min, 180r/min, and shaking culture 10d takes out.4 repetitions are established in each processing.
3, the impact of temperature on Phaeopoms obliquus Crude polysaccharides output
Cultured liquid seeds, with being inoculated in substratum according to volume fraction 10% inoculum size after quick refiner homogenate, is put into respectively to 20 ℃, 25 ℃, 28 ℃, 31 ℃, 36 ℃, and in the shaking table that rotating speed is 150r/min, shaking culture 10d takes out.4 repetitions are established in each processing.
4, plant the impact of age on Phaeopoms obliquus Crude polysaccharides output
Respectively by the liquid seeds of cultivating 5d, 6d, 7d, 8d, 9d with being inoculated in substratum according to volume fraction 10% inoculum size after quick refiner homogenate, be placed in 25 ℃, random alignment in the shaking table that rotating speed is 150r/min, shaking culture 10d taking-up.4 repetitions are established in each processing.
5, the impact of inoculum size on Phaeopoms obliquus Crude polysaccharides output
Cultured liquid seeds, with being inoculated in substratum according to volume fraction 5%, 7.5%, 10%, 12.5%, 15% inoculum size respectively after quick refiner homogenate, is placed in to 25 ℃, random alignment in the shaking table that rotating speed is 150r/min, shaking culture 10d takes out.4 repetitions are established in each processing.
6, the impact of fermentation number of days on Phaeopoms obliquus Crude polysaccharides output
Cultured liquid seeds, with being inoculated in substratum according to volume fraction 10% inoculum size after quick refiner homogenate, is placed in to 25 ℃, random alignment in the shaking table that rotating speed is 150r/min, shaking culture 10d, 11d, 12d, 13d, 14d take out respectively.4 repetitions are established in each processing.
7, orthogonal experiment
The Crude polysaccharides output inside and outside Phaeopoms obliquus born of the same parents of take is index, selects L9(3
4) orthogonal table, take initial pH(A), rotating speed (B), temperature (C), inoculum size (D) determine the best sugared culture condition that produces as direct factor carries out orthogonal experiment.And data are carried out to variance analysis.Then the optimum combination filtering out is verified.
Embodiment 3
The Fuscoporia obliqua polysaccharide extracting after every optimization is carried out to biological activity test, measure respectively the impact (table 3) of inside and outside Polysaccharides on Mice swimming with a load attached to the body time of born of the same parents, impact (table 4) on mice serum blood urea nitrogen (BUN), impact (table 5) on Mouse Blood lactic acid (BLA), the impact (table 6) on Mouse Liver glycogen.The effect that the Fuscoporia obliqua polysaccharide that result proof is extracted has significant enhancing animal endurance, delaying the emergence of fatigue and accelerates elimination of fatigue.
The impact of table 3 Phaeopoms obliquus Crude polysaccharides on the mice burden swimming time
The impact of table 4 Phaeopoms obliquus Crude polysaccharides on mice serum blood urea nitrogen (BUN)
The impact of table 5 Phaeopoms obliquus Crude polysaccharides on Mouse Blood lactic acid (BLA)
The impact of table 6 Phaeopoms obliquus Crude polysaccharides on Mouse Liver glycogen
Specific experiment step is as follows:
1. laboratory animal grouping and polysaccharide dosage setting
Experiment mice is raised in regular grade laboratory, laboratory natural lighting, room temperature 20-25 ℃, well-ventilated.The free pickuping food of animal and drinking-water.
Distillation water as solvent preparation for Phaeopoms obliquus Crude polysaccharides.By healthy mice random packet, be intracellular polyse IO-P1 low dose group 50mg/kg.d, middle dosage group 100mg/kg.d, high dose group 200mg/kg.d; Exocellular polysaccharide IO-P2 low dose group 50mg/kg.d, middle dosage group 100mg/kg.d and high dose group 200 mg/kg.d.Intracellular polyse IO-P3 low dose group 50mg/kg.d, middle dosage group 100mg/kg.d, high dose group 200 mg/kg.d; Exocellular polysaccharide IO-P4 low dose group 50mg/kg.d, middle dosage group 100mg/kg.d and high dose group 200mg/kg.d.Separately establish blank group (distilled water).
Totally 13 groups, 10 every group, between group, body weight is checked without significant difference through t.Take administration by gavage, every day, gavage was 1 time, and dosage is 0.1ml/10g.bw, continuously 30d.Ad lib and drinking-water during gavage.
Method is according in the < < protective foods check of Ministry of Health's issue and evaluation technique standard > > " function of health food is learned assessment process and method of inspection analysis ", and in alleviating physical fatigue functional check method, animal body quality, Loaned swimming test, serum urea are measured, liver starch is measured and blood Plasma lactate step is carried out.
2. the mensuration of Loaned swimming test and swimming time
The continuous gavage 30d of mouse, after last is to tested material 30min, adopting load mode is the sheet lead of 5% body weight at mouse tail root, be placed on water temperature (25 ± 1) ℃, the tank went swimming of the depth of water 30 cm, and with stopwatch, record mouse and from drop into water, start swimming and to being perfectly exhausted, sink to the time that still can not emerge after the 8s of underwater, this time is mouse swimming time.
3. the mensuration of serum urea nitrogen (BUN)
The continuous gavage 30d of mouse, after last is to tested material 30 min, mouse is put in to not swimming with a load attached to the body 90min in the tank of water temperature (25 ± 1) ℃, after rest 60min, from eye socket, get blood approximately 0.5 mL (not adding antithrombotics), put 4 ℃ of refrigerators approximately 3 h, the centrifugal 15min of 2000r/min after hemopexis, gets serum and uses serum urea nitrogen kit measurement serum urea nitrogen value.
4. the mensuration of blood lactic acid (BLA)
The continuous gavage 30d of mouse, after last is to tested material 30 min, mouse orbit is taken a blood sample after 20 μ L, the mouse tail heavy burden 5% water went swimming 10min that puts into 30 ± 1 ℃ of water temperatures is stopped, the 20 μ L that take a blood sample immediately, after swimming, 20min gets blood again.By Lactic acid Kit specification sheets, measure the content of blood lactic acid.In 560nm, measure and respectively manage absorbance, calculate Serum lactic acid content in serum.
5. the mensuration of liver starch
The continuous gavage 30d of mouse, after last is to tested material 30 min, is put in mouse after the water went swimming 60min of 30 ± 1 ℃ of water temperatures, and mouse is put to death in dislocation of cervical vertebra immediately, takes out liver, with filter paper, sucks blood.Accurately take 100 mg, according to liver starch, measure test kit specification sheets and measure hepatic glycogen content.
Experimental result (table 3-table 6) shows that Fuscoporia obliqua polysaccharide energy significant prolongation mouse swimming time shows that Fuscoporia obliqua polysaccharide can obviously improve the exercise tolerance of mouse; Fuscoporia obliqua polysaccharide can significantly reduce the rear mice serum urea nitrogen content of motion, the consumption ratio that Fuscoporia obliqua polysaccharide may be by strengthening the energy supply at the volley of glycogen and fat, reduce protein in muscle makes energy in muscle maintain balance, so that human body strengthens the adaptability of load, can improve the adaptive faculty of body to load, tired elimination accelerated, so the swimming time of polysaccharide group mouse can significant prolongation compared with control group.
After this experiment polysaccharide group mouse movement, hepatic glycogen content is significantly higher than control group, show that Fuscoporia obliqua polysaccharide can increase the liver starch margin of the rear mouse of motion, thereby when organism moves for body provides more energy, thereby the mice burden swimming time obviously extend.
The same with serum urea nitrogen, the accumulation of lactic acid is also the one of the main reasons that causes sports fatigue and cause body sore muscle.Lactic acid is the product of sugared anaerobic glycolysis, and lasting strenuous exercise makes blood lactic acid in body increase by 30 times more than.Lactic acid accumulation too much will affect the stable state of organismic internal environment and the eubolism process in body.The elimination of lactic acid is conducive to tired recovery.This experimental result shows, Fuscoporia obliqua polysaccharide can reduce the blood lactic acid accumulation of the rear mouse of motion, make cell utilize the efficiency of oxygen to improve, thereby strengthened the aerobic metabolism of body, the energy matter that body produces is increased, the generation of delay fatigue, has also explained why the mice burden swimming time can obviously extend from another point of view.
In sum, the experimental group of this research is compared with control group, swimming time significantly improves, serum urea nitrogen and blood lactic acid area under curve obviously reduce, hepatic glycogen content significance improves, and confirms that Fuscoporia obliqua polysaccharide composition has good enhancing animal endurance, delaying the emergence of fatigue, the effect of accelerating elimination of fatigue.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (1)
1. a method of producing polysaccharide with Phaeopoms obliquus fermentation, is characterized in that:
(1) the Phaeopoms obliquus culture medium prescription used that ferments is: Semen Maydis powder 20g/L, glucose 15g/L, wheat bran 30g/L, yeast powder 3g/L, KH
2pO
41g/L, MgSO
4.7H
2o 0.5g/L, V
b10.005g/L, water 1L, pH value is 6;
(2) fermentation condition: rotating speed 140 r/min, 30 ℃ of temperature, fermentation 12d;
(3) Crude polysaccharides extraction step is as follows:
1. in born of the same parents, Crude polysaccharides extracts: filtering fermentation liquor or centrifugal, collect mycelium, and dry, pulverize, take 1 g mycelium powder, add 10-30ml distilled water, heat 0.5-3h at 80-100 ℃, filter, filtrate rotary evaporation, be concentrated into 1/3 of original volume, then add 3 times of volume alcohol, standing 24h, centrifugal, collect flocks;
2. crude extracellular polysaccharide extracts: the fermented liquid that filters mycelia is collected, be concentrated into 1/5 of original volume, add 3 times of volume alcohol, and standing, centrifugal, collect flocks;
3. merge the outer flocks of born of the same parents in born of the same parents, freeze-drying, obtains Crude polysaccharides, water-soluble with phenol sulfuric acid process survey polysaccharide content.
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| CN104086665A (en) * | 2014-07-15 | 2014-10-08 | 江苏阜丰生物科技有限公司 | Method for producing Inonotus obliquus mycelia polysaccharides by high-efficiency extraction and deep liquid fermentation |
| CN104387494A (en) * | 2014-12-06 | 2015-03-04 | 黑龙江众生生物工程有限公司 | Extracting method for water-soluble polysaccharides in inonotus obliquus fruit body |
| CN105349439A (en) * | 2015-12-16 | 2016-02-24 | 黑龙江众生生物工程有限公司 | Culture and fermentation method of inonotus obliquus and preparation method of water-soluble polysaccharides of inonotus obliquus |
| CN105794955A (en) * | 2016-03-21 | 2016-07-27 | 东北农业大学 | Inonotus obliquus selenizing polysaccharide preparation and application of inonotus obliquus selenizing polysaccharide preparation for fresh keeping of raspberries |
| CN107173394A (en) * | 2017-07-18 | 2017-09-19 | 黑龙江省科学院微生物研究所 | Crop growth regulator, preparation method and its usage based on Inonotus obliquus |
| CN107459585B (en) * | 2017-08-30 | 2019-11-19 | 华熙生物科技股份有限公司 | A kind of production method of low molecular weight tremella polysaccharides |
| CN116200273B (en) * | 2022-12-12 | 2024-11-01 | 黑龙江省科学院微生物研究所 | Inonotus obliquus H6 suitable for rapid fermentation and fermentation tank culture method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101805763A (en) * | 2010-03-23 | 2010-08-18 | 王�琦 | Inonotus obliquus extracellular and intracellular mixing crude polysaccharide with function of strengthening immunity |
| CN102038720A (en) * | 2010-12-17 | 2011-05-04 | 中国农业大学 | Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients |
| CN102115350A (en) * | 2010-12-16 | 2011-07-06 | 黑龙江省科学院微生物研究所 | Culture medium and method for submerged fermentation of inonotus obliquus |
| CN102579511A (en) * | 2012-03-13 | 2012-07-18 | 天津大学 | Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus |
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2012
- 2012-09-20 CN CN201210351087.8A patent/CN102898210B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101805763A (en) * | 2010-03-23 | 2010-08-18 | 王�琦 | Inonotus obliquus extracellular and intracellular mixing crude polysaccharide with function of strengthening immunity |
| CN102115350A (en) * | 2010-12-16 | 2011-07-06 | 黑龙江省科学院微生物研究所 | Culture medium and method for submerged fermentation of inonotus obliquus |
| CN102038720A (en) * | 2010-12-17 | 2011-05-04 | 中国农业大学 | Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients |
| CN102579511A (en) * | 2012-03-13 | 2012-07-18 | 天津大学 | Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus |
Non-Patent Citations (4)
| Title |
|---|
| 桦褐孔菌子实体多糖提取研究;高娃 等;《生物技术》;20120430;第22卷(第2期);第70-73页 * |
| 液体发酵生产桦褐孔菌菌丝体及活性成分的研究;潘春丽;《江南大学硕士学位论文》;20080801;第26页倒数第3-8行，第27页倒数第1-4行 * |
| 潘春丽.液体发酵生产桦褐孔菌菌丝体及活性成分的研究.《江南大学硕士学位论文》.2008, |
| 高娃 等.桦褐孔菌子实体多糖提取研究.《生物技术》.2012,第22卷(第2期),第70-73页. |
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