CN101392031A - Extraction and separation method of inonotus obliquus polysaccharide - Google Patents
Extraction and separation method of inonotus obliquus polysaccharide Download PDFInfo
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- CN101392031A CN101392031A CNA2008101374904A CN200810137490A CN101392031A CN 101392031 A CN101392031 A CN 101392031A CN A2008101374904 A CNA2008101374904 A CN A2008101374904A CN 200810137490 A CN200810137490 A CN 200810137490A CN 101392031 A CN101392031 A CN 101392031A
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- phaeopoms obliquus
- fuscoporia obliqua
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Abstract
The invention discloses a method for extracting polysaccharides from medical epiphyte-fuscoporia obliqua, which comprises the step that: fuscoporia obliqua cells are crushed by ultrasonic wave, extracted by hot water, precipitated by frozen ethanol, salted out by ammonium sulfate, dialyzed by a semi-transparent film, sprayed, dried and other processes to obtain the high-purity polysaccharides of the fuscoporia obliqua finally. The extracting method of the invention comprises the methods of ultrasonic wave wall breaking to the raw materials, hot water extracting of the raw material, precipitating the polysaccharides by frozen ethanol for separation, salting-out the polysaccharides by ammonium sulfate for separation, spraying and drying, thereby obtaining high-purity fuscoporia obliqua polysaccharides.
Description
Technical field
The present invention relates to a kind of extracting method of medicinal fungi Fuscoporia obliqua polysaccharide, the special ultrasonic wave of using is carried out break process to the Phaeopoms obliquus cell, uses technologies such as hot water lixiviate, freezing ethanol sedimentation, ammonium sulfate precipitation, semi-permeable membranes dialysis, spraying drying finally to obtain the higher Fuscoporia obliqua polysaccharide of purity.
Background technology
Phaeopoms obliquus [Inonotus obliguus (Fr) Pilat] is that a kind of application will be used fungi very widely on Russia, Poland, Finland and other places, English name chaga.Nineteen fifty-five, chaga is used for treating tumour, particularly Digestive tract and lung tumors by Russian Medicine Research Inst..16th century, the among the people of some countries of Eastern Europe prevented curing cancers with regard to the sclerotium with this bacterium so far.This fungi of Khanty human of western Leah prevents and treats heart trouble, hepatopathy, stomach trouble and esophagus disease etc.Recent research shows, Phaeopoms obliquus can suppress hiv virus (HIV-1), radioprotective and by the biosynthesizing of arrestin matter, antimitotic and eliminate that mechanism of action such as free radical activity suppresses or the growth and the control 0-157 intestinal bacteria that delay tumour cell poison.
The use of Phaeopoms obliquus is mainly based on wild, wild Phaeopoms obliquus mainly be grown in area, Muscovite siberian, Hokkaido, Japan, Finland, etc. ground.There is wild Phaeopoms obliquus to grow in China Changbai mountain, Jilin area and Zhang Guangcai mountain range, Heilungkiang and area, large and small Xing'an Mountains.
Biological study shows that Phaeopoms obliquus is grown very slow under wild state, wild kind is only grown on the birch that lives and just had very high pharmaceutical use in 10～15 years, and therefore wild Phaeopoms obliquus price is very expensive.The U.S. also classifies it as " special natural goods " following drink as the extraterrestrial.Therefore, the research of Phaeopoms obliquus and Products Development have very wide application prospect.
Fungus polysaccharide is the good immunomodulatory toughener of a class, can fundamentally improve the immunologic function of human body, play strengthen the body resistance to consolidate the constitution, the effect of body-building health.Since the eighties in 20th century, the research and utilization of this respect progress more rapidly and just towards anticancer, increase longevity, brute force, intelligence development, beauty treatment and improve immunizing power and prevent aspect in-depth such as aging.Modern medicine study finds that food (medicine) is polysaccharide with the composition that can significantly strengthen cancer patients's resistibility in the bacterium.
Fuscoporia obliqua polysaccharide is except that having the total physiological function of fungus polysaccharide, and its exclusive physiological function also receives much concern, and has unique effect by it aspect anticancer and the inhibition hiv virus.Report is arranged, and Fuscoporia obliqua polysaccharide can reach more than 80% the inhibiting rate of hiv virus.Extraction for Fuscoporia obliqua polysaccharide at present rarely has report.Content of the present invention first system will have the supersonic wave wall breaking method to combine to carry out the extraction of Fuscoporia obliqua polysaccharide, guaranteed the extracted amount and the physiologically active of Fuscoporia obliqua polysaccharide to greatest extent with extraction separation modes such as hot water lixiviate, freezing ethanol sedimentation, ammonium sulfate precipitation, semi-permeable membranes dialysis, spraying dryings.
Summary of the invention
The objective of the invention is to, in the leaching process of Phaeopoms obliquus, use ultrasonic wave that the Phaeopoms obliquus in the Phaeopoms obliquus extracting solution is carried out the broken wall treatment of cell, use extraction that technologies such as hot water lixiviate, freezing ethanol sedimentation, ammonium sulfate precipitation, semi-permeable membranes dialysis, spraying drying carry out Fuscoporia obliqua polysaccharide and separate the higher Fuscoporia obliqua polysaccharide of final acquisition purity.Content, the physiologically active of the polysaccharide that this method is extracted are all higher, are the optimum extracting methods of present Phaeopoms obliquus.
The extraction of polysaccharide of the present invention is achieved in that
1. get to clean at the Phaeopoms obliquus more than 10 years vegetative period and remove surface dirt impurity, 30～55 ℃ of oven dry are pulverized, and cross 20～80 mesh sieve, the water that adds 5～30 times of weight in the Phaeopoms obliquus behind crushing screening soaks the ultrasonication 30～60min that uses 10～30KH after 1～3 hour.
2. keep water temperature to continue for 80～100 ℃ to extract 3～6 hours after-filtration the 1 Phaeopoms obliquus heating of handling, get filtrate.Continuation adds the water of 5～10 times of amounts in filter residue, keep 80～100 ℃ to continue to extract 2～4 hours, filters, and gets filtrate.The water that adds 3～8 times of amounts in the filter residue, 80～100 ℃ continue to extract 1～3 hour, filter.Abandon filter residue, extract the filtrate that obtains with three times and mix, cross ultra-filtration membrane, evaporation concentration is to 1/7～1/5 of original volume.
3. the dehydrated alcohol of getting 0.5～2 times of volume of extracting solution obtains freezing ethanol-20～-5 ℃ of freezing processing 3～5 hours.Obtain Phaeopoms obliquus Crude polysaccharides precipitation with precipitating 10～20 hours in the freezing ethanol adding 2.
4. filter 23 separates sedimentary Crude polysaccharides with filtrate, and refluxes and reclaim the ethanol of removing in the filtrate.In filtrate, add ammonium sulfate and saltout, obtain the precipitation polysaccharide.
5. 3,4 Crude polysaccharides that obtain are mixed, be dissolved in the distilled water, remove ionic impurity in the polysaccharide through the semi-permeable membranes dialysis.
6. after the solution concentration that 5 dialysis are obtained, spraying drying gets Fuscoporia obliqua polysaccharide.
Advantage of the present invention is
The present invention has realized under the situation that less energy consumption, less organic solvent use, the extraction of Fuscoporia obliqua polysaccharide.Adopting ultrasonic wave that raw material is carried out cell wall breaking handles, make the interior polysaccharide component of its cell wall breaking cell in the process of extracting, be extracted out the extraction yield that has increased polysaccharide, adopt freezing ethanol sedimentation and salt precipitation bonded mode to carry out having avoided separating of Fuscoporia obliqua polysaccharide metal ion and impurity protein to sneak in the Fuscoporia obliqua polysaccharide.The extracting method of this invention Fuscoporia obliqua polysaccharide has very strong applicability, is the optimization method that present Fuscoporia obliqua polysaccharide extracts.
Claims (3)
1. the extracting method of a medicinal fungi Fuscoporia obliqua polysaccharide is characterized in that using extraction that supersonic wave wall breaking processing, hot water lixiviate, freezing ethanol sedimentation, ammonium sulfate precipitation, semi-permeable membranes dialysis, spraying drying carry out Phaeopoms obliquus and separates.
According to the requirement of the extraction and separation method of right 1 by the Phaeopoms obliquus after will pulverizing with the back extraction separation acquisition Fuscoporia obliqua polysaccharide after the ultrasonication of the water logging bubble of 3～5 times of amounts.Concrete extraction separation step is as follows:
1) gets vegetative period and remove surface dirt impurity in the cleaning of the Phaeopoms obliquus more than 10 years, 30～55 ℃ of oven dry are pulverized, and cross 20～80 mesh sieve, the water that adds 5～30 times of weight in the Phaeopoms obliquus behind crushing screening soaks the ultrasonication 30～60min that uses 10～30KH after 1～3 hour.
2) keep water temperature to continue for 80～100 ℃ to extract 3～6 hours after-filtration the 1 Phaeopoms obliquus heating of handling, get filtrate.Continuation adds the water of 5～10 times of amounts in filter residue, keep 80～100 ℃ to continue to extract 2～4 hours, filters, and gets filtrate.The water that adds 3～8 times of amounts in the filter residue, 80～100 ℃ continue to extract 1～3 hour, filter.Abandon filter residue, extract the filtrate that obtains with three times and mix, cross ultra-filtration membrane, evaporation concentration is to 1/7～1/5 of original volume.
3) dehydrated alcohol of getting 0.5～2 times of volume of extracting solution obtains freezing ethanol-20～-5 ℃ of freezing processing 3～5 hours.Obtain Phaeopoms obliquus Crude polysaccharides precipitation with precipitating 10～20 hours in the freezing ethanol adding 2.
4) filter 23 separates sedimentary Crude polysaccharides with filtrate, and refluxes and reclaim the ethanol of removing in the filtrate.In filtrate, add ammonium sulfate and saltout, obtain the precipitation polysaccharide.
5) 3,4 Crude polysaccharides that obtain are mixed, be dissolved in the distilled water, remove ionic impurity in the polysaccharide through the semi-permeable membranes dialysis.
6) after the solution concentration that 5 dialysis are obtained, spraying drying gets Fuscoporia obliqua polysaccharide.
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| CNA2008101374904A CN101392031A (en) | 2008-11-10 | 2008-11-10 | Extraction and separation method of inonotus obliquus polysaccharide |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101838341A (en) * | 2010-05-27 | 2010-09-22 | 盐城师范学院 | Method for extracting polysaccharide from limonium sinense kuntze |
| CN102038720A (en) * | 2010-12-17 | 2011-05-04 | 中国农业大学 | Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients |
| CN102106875A (en) * | 2010-12-06 | 2011-06-29 | 浙江工业大学 | Method for extracting water-soluble alpha-glucosidase inhibitor |
| CN102579511A (en) * | 2012-03-13 | 2012-07-18 | 天津大学 | Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus |
| CN102603906A (en) * | 2011-12-30 | 2012-07-25 | 安徽丰原发酵技术工程研究有限公司 | Preparation method of inonotus obliquus polysaccharide aqueous solution |
| CN102949417A (en) * | 2012-11-13 | 2013-03-06 | 中国农业大学 | Application of cryptoporus volvatus in preparing lentivirus inhibitor |
| RU2502516C1 (en) * | 2012-11-08 | 2013-12-27 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Казанский национальный исследовательский технологический университет" (ФГБОУ ВПО "КНИТУ") | Method for producing chromogenic shelf fungus complex |
| CN103690572A (en) * | 2013-12-17 | 2014-04-02 | 杨庆贺 | Preparation method of inonotus obliquus tea powder |
| CN104387494A (en) * | 2014-12-06 | 2015-03-04 | 黑龙江众生生物工程有限公司 | Extracting method for water-soluble polysaccharides in inonotus obliquus fruit body |
| CN107372509A (en) * | 2017-07-18 | 2017-11-24 | 黑龙江省科学院微生物研究所 | A kind of crop strengthening agent and application thereof |
| RU2657431C1 (en) * | 2017-01-23 | 2018-06-13 | Общество с ограниченной ответственностью "Микопро" (ООО "Микопро") | Method for obtaining biologically active substances from fungi |
| CN108175081A (en) * | 2017-12-27 | 2018-06-19 | 吉林省卓轶健康产业有限责任公司 | A kind of efficient complete utilization edible powder of Inonotus obliquus and its production method |
| CN108383920A (en) * | 2018-02-07 | 2018-08-10 | 合肥华盖生物科技有限公司 | A kind of extracting method of Inonotue obliquus |
| CN108902307A (en) * | 2018-06-19 | 2018-11-30 | 青岛农业大学 | A kind of functional yoghourt and preparation method thereof |
| CN109134693A (en) * | 2018-11-19 | 2019-01-04 | 大兴安岭至臻尚品寒带生物技术有限公司 | A kind of preparation method of Fuscoporia obliqua polysaccharide |
| CN110272506A (en) * | 2019-07-22 | 2019-09-24 | 湖北亿彤实业有限公司 | A kind of extracting method of Fuscoporia obliqua polysaccharide |
| CN110357981A (en) * | 2019-07-22 | 2019-10-22 | 湖北亿彤实业有限公司 | A kind of polysaccharide carrier and preparation method thereof mediating function with cell endocytic |
| CN114009647A (en) * | 2021-11-18 | 2022-02-08 | 九愿健康产业科技(苏州)有限公司 | Preparation method of inonotus obliquus granule beverage |
| CN114456943A (en) * | 2020-11-09 | 2022-05-10 | 浙江养生堂天然药物研究所有限公司 | Inonotus obliquus and extract and application thereof |
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2008
- 2008-11-10 CN CNA2008101374904A patent/CN101392031A/en active Pending
Cited By (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101838341A (en) * | 2010-05-27 | 2010-09-22 | 盐城师范学院 | Method for extracting polysaccharide from limonium sinense kuntze |
| CN102106875A (en) * | 2010-12-06 | 2011-06-29 | 浙江工业大学 | Method for extracting water-soluble alpha-glucosidase inhibitor |
| CN102038720A (en) * | 2010-12-17 | 2011-05-04 | 中国农业大学 | Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients |
| CN102038720B (en) * | 2010-12-17 | 2012-05-02 | 中国农业大学 | Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients |
| CN102603906B (en) * | 2011-12-30 | 2014-04-09 | 安徽丰原发酵技术工程研究有限公司 | Preparation method of inonotus obliquus polysaccharide aqueous solution |
| CN102603906A (en) * | 2011-12-30 | 2012-07-25 | 安徽丰原发酵技术工程研究有限公司 | Preparation method of inonotus obliquus polysaccharide aqueous solution |
| CN102579511A (en) * | 2012-03-13 | 2012-07-18 | 天津大学 | Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus |
| CN102579511B (en) * | 2012-03-13 | 2013-11-27 | 天津大学 | Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus |
| RU2502516C1 (en) * | 2012-11-08 | 2013-12-27 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Казанский национальный исследовательский технологический университет" (ФГБОУ ВПО "КНИТУ") | Method for producing chromogenic shelf fungus complex |
| CN102949417A (en) * | 2012-11-13 | 2013-03-06 | 中国农业大学 | Application of cryptoporus volvatus in preparing lentivirus inhibitor |
| CN102949417B (en) * | 2012-11-13 | 2014-08-20 | 中国农业大学 | Application of cryptoporus volvatus in preparing lentivirus inhibitor |
| CN103690572A (en) * | 2013-12-17 | 2014-04-02 | 杨庆贺 | Preparation method of inonotus obliquus tea powder |
| CN103690572B (en) * | 2013-12-17 | 2017-01-04 | 杨庆贺 | Preparation method of inonotus obliquus tea powder |
| CN104387494A (en) * | 2014-12-06 | 2015-03-04 | 黑龙江众生生物工程有限公司 | Extracting method for water-soluble polysaccharides in inonotus obliquus fruit body |
| RU2657431C1 (en) * | 2017-01-23 | 2018-06-13 | Общество с ограниченной ответственностью "Микопро" (ООО "Микопро") | Method for obtaining biologically active substances from fungi |
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| CN108175081A (en) * | 2017-12-27 | 2018-06-19 | 吉林省卓轶健康产业有限责任公司 | A kind of efficient complete utilization edible powder of Inonotus obliquus and its production method |
| CN108383920A (en) * | 2018-02-07 | 2018-08-10 | 合肥华盖生物科技有限公司 | A kind of extracting method of Inonotue obliquus |
| CN108902307A (en) * | 2018-06-19 | 2018-11-30 | 青岛农业大学 | A kind of functional yoghourt and preparation method thereof |
| CN109134693A (en) * | 2018-11-19 | 2019-01-04 | 大兴安岭至臻尚品寒带生物技术有限公司 | A kind of preparation method of Fuscoporia obliqua polysaccharide |
| CN110272506A (en) * | 2019-07-22 | 2019-09-24 | 湖北亿彤实业有限公司 | A kind of extracting method of Fuscoporia obliqua polysaccharide |
| CN110357981A (en) * | 2019-07-22 | 2019-10-22 | 湖北亿彤实业有限公司 | A kind of polysaccharide carrier and preparation method thereof mediating function with cell endocytic |
| CN110357981B (en) * | 2019-07-22 | 2023-08-04 | 福建亿彤生物科技有限公司 | Polysaccharide carrier with endocytosis mediated function and preparation method thereof |
| CN114456943A (en) * | 2020-11-09 | 2022-05-10 | 浙江养生堂天然药物研究所有限公司 | Inonotus obliquus and extract and application thereof |
| CN114456943B (en) * | 2020-11-09 | 2023-12-22 | 浙江养生堂天然药物研究所有限公司 | Inonotus obliquus and extract and application thereof |
| CN114009647A (en) * | 2021-11-18 | 2022-02-08 | 九愿健康产业科技(苏州)有限公司 | Preparation method of inonotus obliquus granule beverage |
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