CN106418111A - Antioxidant extract derived from garlic stalks and preparation method thereof - Google Patents
Antioxidant extract derived from garlic stalks and preparation method thereof Download PDFInfo
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- CN106418111A CN106418111A CN201610874958.2A CN201610874958A CN106418111A CN 106418111 A CN106418111 A CN 106418111A CN 201610874958 A CN201610874958 A CN 201610874958A CN 106418111 A CN106418111 A CN 106418111A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8962—Allium, e.g. garden onion, leek, garlic or chives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
Abstract
The present invention discloses an antioxidant extract derived from garlic stalks and a preparation method thereof, and belongs to the comprehensive utilization technology of the garlic stalks. The obtained extract can be used as the natural antioxidant to be added into food, medicines and cosmetics. The garlic stalks are used as a raw material and the garlic stalk extract having the high antioxidant activity is obtained by the steps of drying, crushing and screening, energy gathered ultrasonic pretreating, compound enzyme digesting, weakly polar macroporous adsorption resin purifying, freezing, freeze-drying, etc. The extracted products are strong in oxidation resistance, simple and high-efficient in extraction method, mild in production conditions, and low in environmental pollution and production costs. The high-efficient utilization of the garlic stalks solves the problem of environmental pollution and also increases the added value of the agricultural products.
Description
Technical field
The present invention relates to technical field of food biotechnology, particularly to a kind of antioxidation from garlic stalk carries
Take thing and preparation method thereof.
Background technology
Substantial amounts of result of study shows, in human body, each organ, system all can produce different degrees of free radical, same in vivo
When there are eliminate oxygen-derived free radicals system of defense, that is, by the antioxidant system of some enzymes and non-enzymatic material composition.But work as machine
After body strenuous exercise, or when being in environmental poisonous substance impact, free radical often generates excessively, or when body is in sick, old and feeble, drink
Eat improper situation lower body and eliminate the reduction of free radical ability, these situations all can be broken between the free radical of body and antioxidant
Balance.Free radical excessively can lead to the popularity infringement of cell and function, so will be by suitable supplemented with exogenous antioxidation
Agent, to improve body antioxidant levels, resists the infringement of free radical.In free radical research field, plant source antioxidant with
Effect of its uniqueness is paid close attention to by everybody.
Bulbus Allii is indispensable flavoring agent in people's life, and has good health care and therapeutic efficiency, such as reduces
Blood fat, pre- preventing thrombosis, anticancer growth, the reduction multiple efficacies such as blood glucose and antioxidation.China produces 11000000 tons of Bulbus Allii per year,
It is important Bulbus Allii manufacturing country and trading country, yield and the volume of trade Jun Zhan world more than 70%.At present, Bulbus Allii harvest Bulbus Allii after,
Straw is all thrown away, and not only wastes resource, pollutes environment again.The composition that garlic stalk contains and Bulbus Allii basic simlarity, no
Contain only volatility garlicin, rich in proteins, saccharide and cellulose, also contain the battalion such as a small amount of phosphorus, potassium/calcium and its vitamin
Form point, but also contain total polyphenols and letones.But domestic at present there is no with antioxidant activity for index separation and Extraction
In garlic stalk, the technology report of oxidation-resistant active ingredient, therefore, extracts the oxidation-resistant active ingredient of garlic stalk, for Bulbus Allii
Straw deep processing and comprehensive utilization, improve its added value, have good market prospect.
Content of the invention
The technical problem to be solved in the present invention is with garlic stalk as raw material, provides a kind of extraction efficiency height, working condition
The preparation method of the gentle and high anti-oxidation activity garlic stalk extract of non-environmental-pollution.The extract of gained is a kind of efficient
The plant origin Natural antioxidant of safety, it can eliminate free radical, suppresses the oxidation of biological composition, can be used as food, medicine
And the Natural antioxidant of cosmetics.
The technical scheme is that, with garlic stalk as raw material, through drying, pulverize and sieve, energy-collecting ultrasonic pretreatment,
Complex enzyme hydrolysis, low pole purification with macroreticular resin, freeze and the step such as lyophilization is so that phenols in garlic stalk, Huang
Ketone and polysaccharide active ingredient fully discharge, thus obtaining a kind of extract with high anti-oxidation activity.One kind is from big
Antioxidant extract of Bulbus Allii straw and preparation method thereof, concrete preparation method is carried out as steps described below：
（1）Garlic stalk raw material, dries to water content about 5% ~ 10%（Mass percent）, then it is cut into the segment of 1 ~ 2 cm,
40 mesh sieves are crossed after pulverizing；
（2）The raw material pulverized is according to solid-liquid ratio 1:15~1:40 addition mass percent concentrations be 80% ethanol, room temperature extraction 6 ~
12 h, then utilize energy-collecting ultrasonic to process 20 ~ 60 min in 20 kHz, 600 W, treatment fluid 4000 rpm is centrifuged 10 min and obtains
Supernatant extract a and precipitation a；
（3）In step（2）Precipitation a in, add that 10 ~ 50 times of volumes contain 0.3% cellulase and the PBS of 0.1% protease delays
Rush liquid, 50 DEG C of constant temperature digest 3 ~ 6 h after 95 DEG C of enzyme denaturing 10 min, and enzymolysis solution is centrifuged after 80% ethanol precipitation, obtains supernatant
Extract b and precipitation b；
（4）By step（2）In supernatant extract a concentrating under reduced pressure to the 1/10 ~ 1/5 of original volume, concentrated solution is through low pole macropore
After resin absorption, first deionized water washes away residual filtrate, then with 80% ethanol elution of 3 ~ 10 times of column volumes, collects eluting
Liquid, is evaporated to paste and freezes after -20 DEG C, obtains freezing thing c；
（5）By step（3）In precipitation b and step（4）In frost thing c respectively at -45 DEG C of lyophilizations, obtain powder e and
Powder f, the two is mixed to get antioxidant extract.
Compared with prior art, the present invention has advantages below：
1. the raw material garlic stalk one side that the present invention adopts is the by-product of agricultural production, on the other hand has and Bulbus Allii phase
As trophic component and preferable physiological function, both solved environmental pollution, improve the added value of agricultural product again.
2. extract prepared in accordance with the present invention, not only yield is high, and bitter to hydroxy radical (OH), hexichol generation
Taste acyl group free radical（DPPH·）With 2,2- azino-two (3- ethyl-benzothiazole -6- sulfonic acid) di-ammonium salts free radical（ABTS）'s
Scavenging activity test result indicate that, garlic stalk extract prepared in accordance with the present invention has fabulous antioxidant activity.
3. the present invention adopt preparation method not through high temperature high pressure process, mainly in combination with energy-collecting ultrasonic and compound enzyme
Solution technology, contributes to macromolecular substances through enzymolysis processing again after ultrasonic pretreatment and is degraded to activating oxide, substantially increase
The extraction efficiency of product, and this process is simple, working condition are gentle, environmental pollution is little, low production cost.
Specific embodiment
The assay method of the Scavenging activity of OH, DPPH and ABTS free radical in the present invention is as follows：
The mensure of OH clearance rate：2 mL sample liquid are taken to sequentially add the FeSO of 2 mL 3 mmol/L4, 2 mL 3 mmol/L
H2O2, 25 DEG C of reaction 10 min, add 2 mL 6 mmol/L salicylic acid, mix standing 15 min, survey its extinction in 510 nm
Value is designated as Ai；Light absorption value when replacing salicylic acid with distilled water is designated as Aj；It is designated as with the light absorption value that distilled water replaces sample liquid
A0.
The mensure of DPPH clearance rate：Take 3 mL sample liquid, add the DPPH solution of 1 mL 0.05 mg/mL, 25 DEG C
React 20 min, 3500 rpm are centrifuged 10 min, taking supernatant to survey its light absorption value in 517 nm is Ai；Replaced with dehydrated alcohol
It is A that DPPH surveys its light absorption valuej；The light absorption value that sample liquid measures is replaced to be designated as A with distilled water0.
The mensure of ABTS clearance rate：4 mL are diluted to the ABTS reactant liquor that light absorption value is 0.7 and 40uL sample through pH7.4PBS
Product liquid mixing 30s, surveys its absorbance A in 734 nm after 6 mini；Light absorption value during ABTS reactant liquor is replaced to be designated as A with PBSj；
A is designated as with the light absorption value that distilled water replaces sample liquid0.
The computing formula of OH, DPPH and ABTS radical scavenging activity is as follows：
Free radical scavenging activity（%）=[1-(Ai- Aj)/A0]×100
With reference to embodiment, the present invention is described in further detail, but the embodiment not limited to this of invention.
Embodiment 1
（1）Garlic stalk raw material, dries to water content about 5%（Mass percent）, then it is cut into the segment of 1 ~ 2 cm, pulverize
Cross 40 mesh sieves afterwards；
（2）The raw material pulverized is according to solid-liquid ratio 1:40 addition mass percent concentrations are 80% ethanol, and room temperature extracts 6 h, then
Process 60 min using energy-collecting ultrasonic in 20 kHz, 600 W, treatment fluid 4000 rpm is centrifuged 10 min and obtains supernatant extract a
With precipitation a；
（3）In step（2）Precipitation a in, add 10 times of PBS containing 0.3% cellulase and 0.1% protease, 50
DEG C constant temperature digests 3 h after 95 DEG C of enzyme denaturing 10 min, and enzymolysis solution be centrifuged after 80% ethanol precipitation, obtain supernatant extract b with
Precipitation b；
（4）By step（2）In supernatant extract a concentrating under reduced pressure to the 1/5 of original volume, concentrated solution is through low pole macroporous resin
After absorption, first deionized water washes away residual filtrate, then with 80% ethanol elution of 10 times of column volumes, collects eluent, decompression
It is concentrated into paste to freeze after -20 DEG C, obtain freezing thing c；
（5）By step（3）In precipitation b and step（4）In frost thing c respectively at -45 DEG C of lyophilizations, obtain powder e and
Powder f, the two is mixed to get antioxidant extract.
Antioxidant extract distilled water is formulated as the sample liquid that concentration is 0.002 g/mL, measure its to OH,
The Scavenging activity of DPPH and ABTS free radical, result is as shown in table 1.
Embodiment 2
（1）Garlic stalk raw material, dries to water content about 10%（Mass percent）, then it is cut into the segment of 1 ~ 2 cm, pulverize
Cross 40 mesh sieves afterwards；
（2）The raw material pulverized is according to solid-liquid ratio 1:15 addition mass percent concentrations are 80% ethanol, and room temperature extracts 8 h, then
Process 20 min using energy-collecting ultrasonic in 20 kHz, 600 W, treatment fluid 4000 rpm is centrifuged 20 min and obtains supernatant extract a
With precipitation a；
（3）In step（2）Precipitation a in, add 30 times of PBS containing 0.3% cellulase and 0.1% protease, 50
DEG C constant temperature digests 6 h after 95 DEG C of enzyme denaturing 10 min, and enzymolysis solution be centrifuged after 80% ethanol precipitation, obtain supernatant extract b with
Precipitation b；
（4）By step（2）In supernatant extract a concentrating under reduced pressure to the 1/6 of original volume, concentrated solution is inhaled through low pole macroporous resin
Attached, first deionized water washes away residual filtrate, then with 80% ethanol elution of 3 times of column volumes, collects eluent, decompression is dense
It is reduced to paste to freeze after -20 DEG C, obtain freezing thing c；
（5）By step（3）In precipitation b and step（4）In frost thing c respectively at -45 DEG C of lyophilizations, obtain powder e and
Powder f, the two is mixed to get antioxidant extract.
Antioxidant extract distilled water is formulated as the sample liquid that concentration is 0.002 g/mL, measure its to OH,
The Scavenging activity of DPPH and ABTS free radical, result is as shown in table 1.
Embodiment 3
（1）Garlic stalk raw material, dries to water content about 7%（Mass percent）, then it is cut into the segment of 1 ~ 2 cm, pulverize
Cross 40 mesh sieves afterwards；
（2）The raw material pulverized is according to solid-liquid ratio 1:20 addition mass percent concentrations are 80% ethanol, and room temperature extracts 12 h, so
Energy-collecting ultrasonic is utilized to process 30 min in 20 kHz, 600 W afterwards, treatment fluid 4000 rpm is centrifuged 10 min and obtains supernatant extract
A and precipitation a；
（3）In step（2）Precipitation a in, add 20 times of PBS containing 0.3% cellulase and 0.1% protease, 50
DEG C constant temperature digests 5 h after 95 DEG C of enzyme denaturing 10 min, and enzymolysis solution be centrifuged after 80% ethanol precipitation, obtain supernatant extract b with
Precipitation b；
（4）By step（2）In supernatant extract a concentrating under reduced pressure to the 1/10 of original volume, concentrated solution is through low pole macroporous resin
After absorption, first deionized water washes away residual filtrate, then with 80% ethanol elution of 4 times of column volumes, collects eluent, decompression
It is concentrated into paste to freeze after -20 DEG C, obtain freezing thing c；
（5）By step（3）In precipitation b and step（4）In frost thing c respectively at -45 DEG C of lyophilizations, obtain powder e and
Powder f, the two is mixed to get antioxidant extract.
Antioxidant extract distilled water is formulated as the sample liquid that concentration is 0.002 g/mL, measure its to OH,
The Scavenging activity of DPPH and ABTS free radical, result is as shown in table 1.
Embodiment 4
（1）Garlic stalk raw material, dries to water content about 8%（Mass percent）, then it is cut into the segment of 1 ~ 2 cm, pulverize
Cross 40 mesh sieves afterwards；
（2）The raw material pulverized is according to solid-liquid ratio 1:30 addition mass percent concentrations are 80% ethanol, and room temperature extracts 10 h, so
Energy-collecting ultrasonic is utilized to process 40 min in 20 kHz, 600 W afterwards, treatment fluid 4000 rpm is centrifuged 10 min and obtains supernatant extract
A and precipitation a；
（3）In step（2）Precipitation a in, add 50 times of PBS containing 0.3% cellulase and 0.1% protease, 50
DEG C constant temperature digests 4 h after 95 DEG C of enzyme denaturing 10 min, and enzymolysis solution be centrifuged after 80% ethanol precipitation, obtain supernatant extract b with
Precipitation b；
（4）By step（2）In supernatant extract a concentrating under reduced pressure to the 1/7 of original volume, concentrated solution is inhaled through low pole macroporous resin
Attached, first deionized water washes away residual filtrate, then with 80% ethanol elution of 6 times of column volumes, collects eluent, decompression is dense
It is reduced to paste to freeze after -20 DEG C, obtain freezing thing c；
（5）By step（3）In precipitation b and step（4）In frost thing c respectively at -45 DEG C of lyophilizations, obtain powder e and
Powder f, the two is mixed to get antioxidant extract.
Antioxidant extract distilled water is formulated as the sample liquid that concentration is 0.002 g/mL, measure its to OH,
The Scavenging activity of DPPH and ABTS free radical, result is as shown in table 1.
Embodiment 5
（1）Garlic stalk raw material, dries to water content about 10%（Mass percent）, then it is cut into the segment of 1 ~ 2 cm, pulverize
Cross 40 mesh sieves afterwards；
（2）The raw material pulverized is according to solid-liquid ratio 1:15 addition mass percent concentrations are 80% ethanol, and room temperature extracts 12 h, so
Energy-collecting ultrasonic is utilized to process 50 min in 20 kHz, 600 W afterwards, treatment fluid 4000 rpm is centrifuged 10 min and obtains supernatant extract
A and precipitation a；
（3）In step（2）Precipitation a in, add 40 times of PBS containing 0.3% cellulase and 0.1% protease, 50
DEG C constant temperature digests 6 h after 95 DEG C of enzyme denaturing 10 min, and enzymolysis solution be centrifuged after 80% ethanol precipitation, obtain supernatant extract b with
Precipitation b；
（4）By step（2）In supernatant extract a concentrating under reduced pressure to the 1/8 of original volume, concentrated solution is inhaled through low pole macroporous resin
Attached, first deionized water washes away residual filtrate, then with 80% ethanol elution of 8 times of column volumes, collects eluent, decompression is dense
It is reduced to paste to freeze after -20 DEG C, obtain freezing thing c；
（5）By step（3）In precipitation b and step（4）In frost thing c respectively at -45 DEG C of lyophilizations, obtain powder e and
Powder f, the two is mixed to get antioxidant extract.
Antioxidant extract distilled water is formulated as the sample liquid that concentration is 0.002 g/mL, measure its to OH,
The Scavenging activity of DPPH and ABTS free radical, result is as shown in table 1.
The oxidation resistance of table 1 extract of the present invention
| Project | OH clearance rate（%） | DPPH clearance rate（%） | ABTS clearance rate（%） |
| Embodiment 1 | 68.2 | 72.5 | 42.9 |
| Embodiment 2 | 71.8 | 62.3 | 46.9 |
| Embodiment 3 | 75.9 | 80.6 | 39.6 |
| Embodiment 4 | 66.8 | 68.2 | 44.8 |
| Embodiment 5 | 80.6 | 76.8 | 50.8 |
Claims (5)
1. a kind of antioxidant extract from garlic stalk and preparation method thereof, the feature of its extract is：With Bulbus Allii straw
Stalk is raw material, prepares a kind of extract with high anti-oxidation activity；This extract has higher removing to free radical
Ability, can be used as the natural anti-oxidation additive of food, medicine and cosmetics.
2. a kind of antioxidant extract from garlic stalk and preparation method thereof, the feature of its preparation method is：According to
Lower step is carried out：
（1）Garlic stalk raw material, dries to water content about 5% ~ 10%（Mass percent）, then it is cut into the segment of 1 ~ 2 cm,
40 mesh sieves are crossed after pulverizing；
（2）The raw material pulverized is according to solid-liquid ratio 1:15~1:40 addition mass percent concentrations be 80% ethanol, room temperature extraction 6 ~
12 h, then utilize energy-collecting ultrasonic to process 20 ~ 60 min in 20 kHz, 600 W, treatment fluid 4000 rpm is centrifuged 10 min and obtains
Supernatant extract a and precipitation a；
（3）In step（2）Precipitation a in, add that 10 ~ 50 times of volumes contain 0.3% cellulase and the PBS of 0.1% protease delays
Rush liquid, 50 DEG C of constant temperature digest 3 ~ 6 h after 95 DEG C of enzyme denaturing 10 min, and enzymolysis solution is centrifuged after 80% ethanol precipitation, obtains supernatant
Extract b and precipitation b；
（4）By step（2）In supernatant extract a concentrating under reduced pressure to the 1/10 ~ 1/5 of original volume, concentrated solution is through low pole macropore
After resin absorption, first deionized water washes away residual filtrate, then with 80% ethanol elution of 3 ~ 10 times of column volumes, collects eluting
Liquid, is evaporated to paste and freezes after -20 DEG C, obtains freezing thing c；
（5）By step（3）In precipitation b and step（4）In frost thing c respectively at -45 DEG C of lyophilizations, obtain powder e and
Powder f, the two is mixed to get antioxidant extract.
3. the preparation method of a kind of antioxidant extract from garlic stalk according to claim 2, its feature exists
In：Step（3）Described in precipitation a carry out enzymolysis processing using the PBS containing cellulase and protease.
4. the preparation method of a kind of antioxidant extract from garlic stalk according to claim 2, its feature
It is：Step（4）Described in supernatant extract a concentrate after processed using low pole macroporous resin adsorption and ethanol elution, enter
One step concentrates as freezing after paste.
5. the preparation method of a kind of antioxidant extract from garlic stalk according to claim 2, its feature exists
In：Step（5）Described in precipitation b and frozen material c through -45 DEG C of lyophilizations, obtain powder e and powder f, the two is mixed to get
Antioxidant extract.
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Cited By (5)
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| CN108634313A (en) * | 2018-05-11 | 2018-10-12 | 江苏大学 | A kind of sweet potato stem leaf high nutrition activity extract and preparation method thereof |
| CN110755532A (en) * | 2019-11-12 | 2020-02-07 | 漯河市一墨池医药科技有限责任公司 | Method for extracting garlic skin, garlic skin extract obtained by extraction and application of garlic skin extract |
| CN112868908A (en) * | 2021-02-15 | 2021-06-01 | 巨野恒丰果蔬有限公司 | Method for extracting effective components of garlic straw by biological enzymolysis water |
| CN113355156A (en) * | 2021-06-30 | 2021-09-07 | 四川省食品发酵工业研究设计院有限公司 | Antioxidant, preparation method thereof and application thereof in edible oil |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108260480A (en) * | 2018-01-06 | 2018-07-10 | 佛山市所能网络有限公司 | A kind of passion fruit and its implantation methods with anti-oxidation function |
| CN108634313A (en) * | 2018-05-11 | 2018-10-12 | 江苏大学 | A kind of sweet potato stem leaf high nutrition activity extract and preparation method thereof |
| CN110755532A (en) * | 2019-11-12 | 2020-02-07 | 漯河市一墨池医药科技有限责任公司 | Method for extracting garlic skin, garlic skin extract obtained by extraction and application of garlic skin extract |
| CN112868908A (en) * | 2021-02-15 | 2021-06-01 | 巨野恒丰果蔬有限公司 | Method for extracting effective components of garlic straw by biological enzymolysis water |
| CN113355156A (en) * | 2021-06-30 | 2021-09-07 | 四川省食品发酵工业研究设计院有限公司 | Antioxidant, preparation method thereof and application thereof in edible oil |
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| CN106418111B (en) | 2019-12-03 |
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