CN105901705A - Compound preparation of edible mushroom polysaccharides and selenium-rich rice protein peptides and preparation method thereof - Google Patents
Compound preparation of edible mushroom polysaccharides and selenium-rich rice protein peptides and preparation method thereof Download PDFInfo
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- CN105901705A CN105901705A CN201610248830.5A CN201610248830A CN105901705A CN 105901705 A CN105901705 A CN 105901705A CN 201610248830 A CN201610248830 A CN 201610248830A CN 105901705 A CN105901705 A CN 105901705A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/12—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses
- A23J1/125—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses by treatment involving enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a compound preparation of edible mushroom polysaccharides and selenium-rich rice protein peptides and a preparation method thereof. The preparation includes 55-70 wt% of edible mushroom polysaccharide powder and 30-45 wt% of rice selenium-rich protein powder. The preparation method includes the steps of: ultrasonic extraction of edible mushroom polysaccharides, preparation of selenium-rich rice protein, nutritional compounding of the edible mushroom polysaccharide powder and the rice selenium-rich protein powder according to the weight ratio, and capsule filling with the mixture by means of a precise capsule filling plate to obtain a capsule finish product. The compound preparation not only effectively utilizes rice bran resources but also achieves selenium reinforcement, not only overcomes a defect that the edible mushroom polysaccharide has poor activities, such as anti-oxidization and the like, but also solves the problems that the selenium-rich rice protein peptide is poor in immuno-competence. The polysaccharides and the selenium-rich protein peptides are matched with each other to integrate the bio-effects thereof, so that the compound preparation satisfies requirement in multiple aspects of functional health-caring products by people at present.
Description
Technical field
The present invention relates to technical field of health care food, be specifically related to a kind of edible fungi polysaccharide and the compounding system of rice rich in selenium protein peptide
Agent and preparation method thereof.
Background technology
Edible and medical fungi is one of important vegetable protein and nutritional factors source of people's daily life, and its function is the heaviest
Depending on.Common are: Ganoderma, Lentinus Edodes, Hericium erinaceus (Bull. Ex Fr.) Pers., Coriolous Dersicolor (Fr.) Quel, Flammulina velutiper (Fr.) Sing, Volvariella volvacea (Bull.Ex Franch.) Singer．, Agaricus bisporus, Auricularia, Tremella, Pleurotus eryngii etc..Food
Medicinal fungus planting technique cost is relatively low, delicious flavour, nutritious, containing abundant aminoacid, protein, saccharide, vitamin,
The multiple nutritional components such as mineral element, nucleotides, is a kind of important industry in China's agricultural, is also to be only second in plant husbandry
Grain, cotton, oil, really, the sixth-largest industry of dish.China is that world's edible and medical fungi produces the first big country, according to statistics, whole nation food in 2006
Medicinal fungus production has reached 14,000,000 tons, within 2007, breaks through 15,000,000 tons, and year creates the output value about 60,000,000,000 yuans, accounts for the world
More than the 70% of total output.In recent years, existing modern medicine and pharmacology research shows that some edible and medical fungi is because having medical value widely
And be widely used, its function mainly has regulation immunity of organism level, to liver, the protective effect of cardiovascular system, gives protection against cancer, resists
Virus, antibacterial and anti-inflammation functions, have eliminating phlegm and stopping cough, stomach invigorating is aid digestion, relieving constipation diuresis and hypotensive activity etc..Edible and medical fungi
Healthy nutritive value is high, rich in abundant trophic function composition.Along with the nutritional labeling of edible and medical fungi, chemical composition, biology
Activity and mechanism of action thereof are the most revealed, and its edibility, health care and medical value are extremely paid attention to.Numerous studies table
Bright, edible and medical fungi contains abundant polysaccharide, functional protein, triterpene, flavone, polyphenol, saponin, nucleoside etc. and has bioactive
Compound, has enhancing immunity, alleviating physical fatigue, auxiliary effect such as blood fat reducing, slow down aging, and relative inexpensiveness.
Abroad, some edible and medical fungi polysaccharide have been developed that into capsule and the tablet of raising immunity, also have some to be developed to
The additive of functional food beverage.The materials such as the saponin that contains in multiple edible and medical fungi, terpenoid, Polyphenols, have significantly
Lipid-lowering effect.Particularly Lentinus Edodes can reduce blood plasma lipide, cholesterol and glycerol three containing eritadenine active component
Ester.Additionally, the Flavonoid substances that edible and medical fungi contains to antioxidation, anti peroxidation of lipid, blood sugar lowering, protect the liver and press down cancer and anti-cancer
There is certain prevention and auxiliary therapeutic action.
The most domestic functional food limited amount with edible and medical fungi functional factor as development of raw materials, the market share is relatively
Few, and functional component is indefinite, quality standard imperfection, this just greatly limit edible and medical fungi functional food and opens
The development of the industry of sending out.In China's edible and medical fungi industry, intensive processing is the weakest link, and its technology, process equipment are the most remote
Far lagging behind developed country, at present, major part processing enterprise also rests on the rough machined levels such as fresh-keeping, drying, salting, product
Mass discrepancy is big, and added value of product is low, it is impossible to adapt to domestic and international market to food safety and the requirement of nourishing healthy.Edible and medical fungi
Process technology is researched and developed delayed situation and China's edible and medical fungi industry is in a disadvantageous position, only in the competition of international market
It is dominant with quantity, because the trade dispute of safety issue, quality problems is continuous, product inexpensive, it is impossible to promote industry water
Flat.Meanwhile, the integral innovative ability of edible and medical fungi industry is not enough, and the deposit of new product and correlation technique is the deficientest.At present, state
The interior functional food major part with edible and medical fungi as development of raw materials is immunoregulatory functional food, and its functional factor is mainly
Active polysaccharide.But the health promoting product with polysaccharide as functional factor of state approval is all polysaccharide mixture now, the content of polysaccharide
It is generally lower than 30%.Existing research has been found that multiple edible and medical fungi polysaccharide has regulation immunity, antioxidation, blood sugar lowering, fall blood
The functions such as pressure, but be correlated with and go deep into and systematic research work still deficiency, polysaccharide mechanism of action is the most indefinite.Therefore, food is strengthened
Be correlated with deep processed product Research intensity and industrialization paces of medical fungus polysaccharide are the most necessary.
Selenium (Selenium) is a kind of trace element necessary to human and animal's growth promoter, and selenium and selenium protein have anti-
The biological functions such as Oxidation, enhancing human body immunity ability and prevention of various diseases.But, China have 73% Selenium in Soil dense
Degree, less than 0.02mg/kg, belongs to scarce selenium and low selenium area, result in Selenium In Foods content too low.Dietary survey shows for the second time
It is 26mg/kg that crowd takes in selenium, the 50-200mg/kg recommended well below RDA.Epidemiological survey shows China Jiangsu and Hei Long
The hepatocarcinoma of Jiang Sheng selenium all low with meals with the sickness rate of Keshan disease is relevant, after meals add selenium, and the incidence rate of cancer and mortality rate
Significantly reduce.Selenium supplement is significant for improving people's health level.Selenium in natural food is insufficient for human body
Normal need, economic, efficient, safe selenium-enriched food is the effective way of mankind's selenium supplement.The academician such as Lu Liangshu, Li Zhensheng refer to
Going out, improving food chain selenium level is to regulate and control selemium nutrition from source and prevent the fundamental solution about selenium deficiency disease, and appeals to add
Strong Se-rich farm products exploitation, is produced by common crops and is organised by selenium, set up mankind's selemium nutrition pattern of conventional food picked-up.
The exploitation of Se-rich farm products technology, provides a practicable food selenium supplement approach for scarce selenium area crowd.Due to commonly
Food selenium content is relatively low, can not have been met human body selenium intake by edible various kinds of foods.China starts to lead to from the eighties
Cross the strengthening salt of inorganic selenium to improve the intake of human body selenium.But numerous studies prove that directly adding of inorganic selenium causes body thin
The damage of born of the same parents.Chinese scholars be sought for a kind of high Se content biological activity is higher but compound that cytotoxicity is low and food
Product.Similar to sulfur assimilation approach, the inorganic selenium in soil can be converted into organic selenium compounds by plant.The nineties initial stage, beautiful
It is 100-1300 g kg-1 that the concentration of state scholar Ip and Lisk control selenite and frequency of administration successfully nurture selenium content
The selenium-enriched garlic of (dry).Abroad in succession research and develop selenium-rich Caulis et Folium Brassicae capitatae, Bulbus Allii Cepae, Herba Alii fistulosi class, Rhizoma Solani tuber osi, Fructus Hordei Germinatus and Semen Tritici aestivi.Domestic
Also the research of plant rich in selenium is started in the nineties initial stage, to improve the selenium intake of crowd.Have developed Selenium rich rice, selenium-rich
Semen sojae atricolor, selenium enriched Spirulina, Se-rich lucid ganoderma, Fructus Hordei Germinatus rich in selenium and Folium Camelliae sinensis rich in selenium, the quality of plant significantly improves, and selenium content is notable
Increasing, this is to scarce selenium area crowd's selenium supplement and prevents all kinds of tumors to have great significance.
Oryza sativa L. is one of the most most important crops, the long-term Monitoring of Paddy Rice Plant Area of China about 4.5 hundred million mu, always produces
About 1.9 hundred million tons, account for the whole nation plant of grain crops area 30% and total output 40%.There is more than 60% population in the whole nation with rice
For staple food, nearly 1.8 hundred million tons of Oryza glutinosa of year consumption.Therefore, rice, for the crowd with it as staple food, is a kind of critically important meals
Food selenium source.Currently, the biological reinforced technique of enriched selenium of rice is concentrated mainly on three below aspect: (1) is by using external source selenium
Improve the Se content in rice；(2) filter out selenium-rich parent, select selenium-enriched rice kind；(3) to the heredity of selenium in rice
Property is analyzed.Meanwhile, develop application aspect at Selenium rich rice, mainly use and enrich the method for external source selenium to improve in rice
Se content.According to the rice physiological absorption to trace element-selenium and the feature of metabolism, study and develop easily by rice from absorbing
Fertilizer rich in selenium, applied by soil root that selenium is fertile or foliage-spray selenium fertile, improve the accumulation at Rice Kernel position of the selenium element, raw
Output Se content is apparently higher than the selenium-enriched rice of matched group.During rice is planted, apply selenium-enriched fertilizer, not only can improve rice
The content of middle selenium, moreover it is possible to improve yield of brown rice and improve its quality, strengthens rice resistance simultaneously and alleviates heavy metal in soil
Toxic action to rice.The results show of Wei Dan etc., imposes a certain amount of selenium fertilizer of finite concentration, no in scarce selenium area
But the Se content of Rice Kernel can be improved, and yield of brown rice also can improve 8.1% to 18.0%.After rice applies selenium-enriched fertilizer,
Its morning disease-resistant, anti-, cold-resistant, antioxidation, solution poisoning can be increased, solve the functions such as frost.Studies have found that foliage-spray selenium fertilizer can be same
The SOD antioxidase of Shi Zengqiang rice seedling and the activity of POD antioxidase.Selenium element can also be effectively improved rice quality, because of
It can affect the level of rice body some organic compound interior.Zhou Xinbin etc., by spraying sodium selenite, make selenium-enriched rice seed
The Se content of grain, between 0.225 to 0.586 mg/kg, is 8 to 10 times of matched group, and wherein organic selenium content accounts for total selenium
90%.The Se content that foliage-spray selenium fertilizer is proved to be remarkably improved in rice is carried out at Grain Filling, and selenium-enriched rice
Water extract becomes dose relationship and has relatively high antioxidant with the content of selenium.Having animal test results to show, mice feeds selenium-rich
After rice, GPx enzymatic activity is significantly improved, and has good antimutagenic effect.In order to further appreciate that selenium in selenium-enriched rice
In vivo with the activity mechanism of external performance, the form that in selenium-enriched rice, selenium exists need to be studied.Scholar is had to utilize diaminonaphthalene glimmering
Optical analysis measures the content of organic selenium, test result indicate that, in selenium-enriched rice, organic selenium content is more than 99%, additionally, seleno first
Methyllanthionine (SeMet) accounts for about the 50% of organic selenium.The enzyme hydrolysis method such as Zhang extracts seleno-amino acids in selenium-enriched rice, anti-phase
Paired-ion chromatography-ICPMS detected a main unknown peak, but does not find selenomethionine (SeMet), causes
The reason of this result is probably that hydrolysis time is long causes SeMet oxidized.Williams etc. use μ-XANES(x radiation absorption
Near edge structure) form of selenium in selenium-enriched rice is analyzed and identifies, test result indicate that by technology, except containing in selenium-enriched rice
Outside 39.2% SeMet, the MeSeCys (MeSeCys) possibly together with 55.3%, meanwhile, this author points out that the form of selenium is divided
Analysis needs distinct methods to verify.
Rice protein is the vegetable protein of a kind of high-quality, after enzyme hydrolysis, not only will not reduce the nutriture value of protein
Value, moreover it is possible to obtain active polypeptide more more than original protein and aminoacid.Prepared by rice by-product rice residue
Biologically active peptide have antioxidation, regulation immunity, auxiliary reduce blood pressure, alleviate atherosclerosis and strengthen food wind
The functions such as taste.Most researchs prove that rice protein hydrolysates have abilities removing DPPH free radical, and its Scavenging activity with
Dose-effect relationship is there is between the concentration of rice protein hydrolysate.Zhang etc. are from by 5 kinds of different protease hydrolysis rice
Albumen, and 2 kinds of high activity anti-oxidation peptides of isolated and purified acquisition, and during flight substance assistant laser desorpted ionized with MALDI-TOF(
Between mass spectrum) identify primary structure, be the Phe-Arg-Asp-Glu-His-Lys-Lys and 1002.5 Da of 959.5 Da respectively
Lys-His-Asp-Arg-Gly-Asp-Glu-Phe fragments of peptides.The immunocompetence of rice active peptide is the most studied by majority
Prove, the functional food with regulation immunization the most ratified by China's Ministry of Public Health.Chen etc. are sick by human white blood
The foundation of cell U937 model, it was demonstrated that the albumin obtained from rice has human activin antileukemie immunne response merit
Energy.In rice albumin is studied, Takahashi etc. has separated a kind of rice active peptide Oryzatensin of acquisition, and structure is
Gly-Tyr-Pro-Met-Tyr-Pro-Leu-Arg, has immunoregulatory activity, when concentration is 1 μm ol/L, and phagocytosis
Substantially, phagocytic index is 1.5 times of controlling value.Theoretical according to complementary action of protein, the food proteins of separate sources is by a certain percentage
Mixing complements each other, and can improve its nutritive value.1973, the World Health Organization (WHO) and FAO (Food and Agriculture Organization of the United Nation) (FAO) carried
Go out the essential amino acid pattern evaluating proteinaceous nutrient.Zhao Jiang etc. use chemical score, AAS and amino acid ratio
The Nutritive evaluation method that Y-factor method Y etc. are international, determines the whey protein concentrate in egg albumen powder respectively and Semen sojae atricolor is divided
From the crude protein content of albumen, and determining its aminoacid composition, the nutritive value to them has carried out comprehensively commenting simultaneously
Valency.Fuzzy Recognition and the amino acid ratio Y-factor method Y such as Liu Fang, form the aminoacid of 3 kinds of leaf of locust albumen and content carried out
Analyze, and the nutritive value of these 3 kinds of albumen carried out thoroughly evaluating and has compared.Guo Jie is by force etc. with containing strengthening ratio accordingly
Amino acid whose defatted soy flour supplement rice and carry out zoopery, but experiment in protein digestibility relatively low, Semen sojae atricolor to be improved
Utilization rate, its aminoacid composition and the problem such as processing technique should be considered comprehensively.
Therefore, a kind of new technical scheme of offer is provided badly and solves above-mentioned edible fungi polysaccharide and the nutrition of rice selenium-enriched protein peptide
Compounding problem, i.e. a kind of edible fungi polysaccharide and rice rich in selenium protein peptide complex preparation and preparation method thereof.
Summary of the invention
, problem to be solved
For the above-mentioned problems in the prior art, the present invention provides a kind of edible fungi polysaccharide and the compounding system of rice rich in selenium protein peptide
Agent and preparation method thereof, by being compounded by selenium-enriched protein peptide in polysaccharide component in edible fungi and rice, has both been effectively utilized Testa oryzae
Resource serves again the effect of strengthening selenium element, both can supplement the more weak shortcoming of antioxidation isoreactivity of edible fungi polysaccharide, again may be used
To eliminate rice selenium-enriched protein peptide deficiency in terms of immunocompetence, such that it is able to make the biology of various polysaccharide and selenium-enriched protein peptide
Effect cooperates, and meets Modern human populations demand many to functional health product.
, technical scheme
In order to solve the problems referred to above, the technical solution adopted in the present invention is as follows:
Described a kind of edible fungi polysaccharide and rice rich in selenium protein peptide complex preparation, described preparation includes the edible fungi of 55 ~ 70 wt%
The rice egg rich in selenium white lead of polysaccharide powder and 30 ~ 45 wt%.
Preferably, described preparation includes the edible fungi polysaccharide powder of 65 wt% and the rice egg rich in selenium white lead of 35 wt%.
Preferably, the purity of described edible fungi polysaccharide powder and rice egg rich in selenium white lead is all not less than 85%.
Preferably, described edible fungi include Ganoderma, Lentinus Edodes, Hericium erinaceus (Bull. Ex Fr.) Pers., Coriolous Dersicolor (Fr.) Quel, Flammulina velutiper (Fr.) Sing, Volvariella volvacea (Bull.Ex Franch.) Singer．, Agaricus bisporus, Auricularia,
Tremella and Pleurotus eryngii.
Preferably, described preparation is tablet, pill, capsule or oral liquid.
Preferably, described preparation is capsule.
A kind of edible fungi polysaccharide and the preparation method of rice rich in selenium protein peptide complex preparation, ultrasonic including edible fungi polysaccharide carries
Take, the preparation of rice selenium-enriched protein and according to above-mentioned ratio by prepared edible fungi polysaccharide powder, rice egg rich in selenium white lead
Carry out nutrition to compound, fill plate with accurate capsule and capsule is filled with, collect capsule and obtain edible fungi polysaccharide and rice selenium-rich
Nutrient protein compounds capsule finished product.
Preferably, the supersound extraction of described edible fungi polysaccharide comprises the steps:
A1. by fresh food bacterium dry in the sun to after the most half-dried, it is put in baking oven air blast under the conditions of 60 DEG C and dries, then pulverize through 30B
Machine is pulverized, and double-roll crusher crushes, and 80 mesh sieves divide, and jet mill is pulverized, and 300 mesh sieves divide, and prepares edible fungi superfine powder, puts
Standby in-20 DEG C；
A2. weigh described edible fungi superfine powder, add 85% appropriate ethanol and stand 2h at room temperature, with remove partial pigment and
Impurity, adds 420mL deionized water according still further to ratio of water to material 28mL/g and fully mixes, carry with DCTZ-2000 multipurpose thermostatic ultrasonic
The machine that takes ultrasonic 15 ~ 20min under power is 680W, obtains lixiviating solution；
A3. take step A2 gained lixiviating solution centrifugal 15min under the speed of 5000r/min, take supernatant i.e. supernatant one, described on
The Sevag reagent method Deproteinization that clear liquid one uses n-butyl alcohol and chloroform volume ratio to be 1:4, and under the conditions of 8000r/min
Centrifugal 10min, takes supernatant i.e. supernatant two；
A4. being put by described supernatant two and be evaporated under the conditions of 50 DEG C in Rotary Evaporators in right amount, concentrated solution adds 4 times of volumes
Dehydrated alcohol is refrigerator precipitation 12h under the conditions of 4 DEG C；
A5. take step A4 products therefrom and be centrifuged 15min taking precipitate in 5000r/min, and precipitate puts 60 DEG C of oven for drying, i.e.
The edible fungi polysaccharide of available ultrasonic extraction.
Preferably, Rotary Evaporators described in step A4 is N-1100EYELA type Rotary Evaporators.
Preferably, the preparation of described rice selenium-enriched protein comprises the steps:
B1. weighing 5g rice dry powder in 400ml beaker, add 250 ml, 0.1 M, pH value is that the PBS buffering of 6.6-7.0 is molten
Liquid, adding cellulase with pectase mass ratio is the mixed enzyme of 7:3, stirs, keeps away in the water-bath of 30-40 DEG C of condition
Light extraction 8 hours；
B2. by the step B1 products therefrom filtered through gauze of 100-200 mesh, collect clear liquid one, progressively add in described clear liquid one
Enter analytically pure solid (NH4)2SO4To final concentration of 20%；
B3. step B2 products therefrom is saltoutd 10-14 hour, and with the centrifugation 10min of 8000 r/min, remove miscellaneous egg
In vain, clear liquid two is obtained；
B4. in described clear liquid two, it is gradually added analytically pure solid (NH4)2SO4To final concentration of 45%；
B5. step B4 products therefrom is saltoutd 12 hours, and the centrifugation 10min of 8000 r/min, remove clear liquid, will precipitation
Dissolve, dialyse 24 hours with the bag filter flowing water that molecular weight is 3000, then dialyse 24 hours with deionized water, within every 4 hours, change one
Secondary water, by the solution constant volume in bag filter to 200 ml, detection i.e. obtains rice selenium-enriched protein.
, beneficial effect
Compared to prior art, the invention have the benefit that
(1) present invention utilizes judder that ultrasound wave produces, high acceleration, strong cavitation effect, stirring action etc., it is possible to
Strengthen the release of intracellular organic matter, spread and dissolve, thus improve polysaccharide yield, shorten extraction time；Ultrasonic disruption simultaneously
Process is a physical process, without chemical reaction in leaching process, affects little on the biological activity of active substance；
(2) present invention utilizes micronizing, ultrasound assisted extraction, enzyme process assisted extraction, Ultra filtration membrane, lyophilization, spraying
The technology such as be dried selenium-enriched protein peptide in polysaccharide component in edible fungi and rice is carried out technique is simple, extraction efficiency is high separation and
Purification, and according to certain certain proportioning mixing, capsules preparation technique prepares edible fungi polysaccharide and rice selenium-enriched protein routinely
The nutrition of peptide compounds capsule, both can supplement the more weak shortcoming of antioxidation isoreactivity of edible fungi polysaccharide, can eliminate again rice rich
Selenium protein peptide deficiency in terms of immunocompetence, such that it is able to make the biological effect phase interworking of various polysaccharide and selenium-enriched protein peptide
Close, meet Modern human populations demand many to functional health product；
(3) present invention is according to food protein complementarity principle, is compounded by selenium-enriched protein peptide in polysaccharide component in edible fungi and rice,
Not only it is effectively utilized Testa oryzae resource but also serve the effect of strengthening selenium element.In rice, lysine (Lys) is the first restriction amino
Acid, and in edible fungi, in polysaccharide component, lysine content, than abundant, therefore adds polysaccharide group in edible fungi in rice bran protein
Point so that it is nutrition is more comprehensively；And also there is the advantages such as energy consumption is low, single-stage separation efficiency high, equipment is simple, pollution-free.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described below.
Embodiment 1
A kind of edible fungi polysaccharide and rice rich in selenium protein peptide complex preparation, described preparation includes the edible fungi polysaccharide powder and 35 of 65 wt%
The rice egg rich in selenium white lead of wt%.The purity of described edible fungi polysaccharide powder and rice egg rich in selenium white lead is all not less than 85%.
It should be noted that described edible fungi includes Ganoderma, Lentinus Edodes, Hericium erinaceus (Bull. Ex Fr.) Pers., Coriolous Dersicolor (Fr.) Quel, Flammulina velutiper (Fr.) Sing, Volvariella volvacea (Bull.Ex Franch.) Singer．, double spore mushroom
Mushroom, Auricularia, Tremella and Pleurotus eryngii.As preferably, described preparation is capsule.
A kind of edible fungi polysaccharide and the preparation method of rice rich in selenium protein peptide complex preparation, ultrasonic including edible fungi polysaccharide carries
Take, the preparation of rice selenium-enriched protein and according to above-mentioned ratio by prepared edible fungi polysaccharide powder, rice egg rich in selenium white lead
Carry out nutrition to compound, fill plate with accurate capsule and capsule is filled with, collect capsule and obtain edible fungi polysaccharide and rice selenium-rich
Nutrient protein compounds capsule finished product.
The supersound extraction of described edible fungi polysaccharide comprises the steps:
A1. by fresh food bacterium dry in the sun to after the most half-dried, it is put in baking oven air blast under the conditions of 60 DEG C and dries, then pulverize through 30B
Machine is pulverized, and double-roll crusher crushes, and 80 mesh sieves divide, and jet mill is pulverized, and 300 mesh sieves divide, and prepares edible fungi superfine powder, puts
Standby in-20 DEG C；
A2. weigh described edible fungi superfine powder, add 85% appropriate ethanol and stand 2h at room temperature, with remove partial pigment and
Impurity, adds 420mL deionized water according still further to ratio of water to material 28mL/g and fully mixes, carry with DCTZ-2000 multipurpose thermostatic ultrasonic
The machine that takes ultrasonic 19.8min under power is 680W, obtains lixiviating solution；
A3. take step A2 gained lixiviating solution centrifugal 15min under the speed of 5000r/min, take supernatant i.e. supernatant one, described on
The Sevag reagent method Deproteinization that clear liquid one uses n-butyl alcohol and chloroform mol ratio to be 1:4, and under the conditions of 8000r/min
Centrifugal 10min, takes supernatant i.e. supernatant two；
A4. described supernatant two is put and N-1100EYELA type Rotary Evaporators is evaporated under the conditions of 50 DEG C in right amount, dense
Contracting liquid adds 4 times of volume dehydrated alcohol refrigerator precipitation 12h under the conditions of 4 DEG C；
A5. take step A4 products therefrom and be centrifuged 15min taking precipitate in 5000r/min, and precipitate puts 60 DEG C of oven for drying, i.e.
The edible fungi polysaccharide of available ultrasonic extraction.
The preparation of described rice selenium-enriched protein comprises the steps:
B1. weighing 5g rice dry powder in 400ml beaker, add 250 ml, 0.1 M, pH value is that the PBS buffering of 6.6-7.0 is molten
Liquid, adding cellulase with pectase mass ratio is the mixed enzyme of 7:3, stirs, keeps away in the water-bath of 30-40 DEG C of condition
Light extraction 8 hours；
B2. by the step B1 products therefrom filtered through gauze of 100-200 mesh, collect clear liquid one, progressively add in described clear liquid one
Enter analytically pure solid (NH4)2SO4To final concentration of 20%；
B3. step B2 products therefrom is saltoutd 10-14 hour, and with the centrifugation 10min of 8000 r/min, remove miscellaneous egg
In vain, clear liquid two is obtained；
B4. in described clear liquid two, it is gradually added analytically pure solid (NH4)2SO4To final concentration of 45%；
B5. step B4 products therefrom is saltoutd 12 hours, and the centrifugation 10min of 8000 r/min, remove clear liquid, will precipitation
Dissolve, dialyse 24 hours with the bag filter flowing water that molecular weight is 3000, then dialyse 24 hours with deionized water, within every 4 hours, change one
Secondary water, by the solution constant volume in bag filter to 200 ml, detection i.e. obtains rice selenium-enriched protein.
Based on above-mentioned, the present invention utilizes judder that ultrasound wave produces, high acceleration, strong cavitation effect, stirring
Effect etc., it is possible to strengthen the release of intracellular organic matter, spread and dissolve, thus improve polysaccharide yield, shorten extraction time；Simultaneously
Ultrasonic disruption process is a physical process, without chemical reaction in leaching process, affects little on the biological activity of active substance；
By utilizing the technology such as micronizing, ultrasound assisted extraction, enzyme process assisted extraction, Ultra filtration membrane, lyophilization, spray drying
Selenium-enriched protein peptide in polysaccharide component in edible fungi and rice is carried out separation and the purification that technique is simple, extraction efficiency is high, and presses
According to certain certain proportioning mixing, capsules preparation technique prepares the nutrition of edible fungi polysaccharide and rice selenium-enriched protein peptide again routinely
Join capsule, both can supplement the more weak shortcoming of antioxidation isoreactivity of edible fungi polysaccharide, rice selenium-enriched protein peptide can be eliminated again and exist
Deficiency in terms of immunocompetence, such that it is able to make various polysaccharide cooperate with the biological effect of selenium-enriched protein peptide, meets the modern times
Crowd's demand many to functional health product；By according to food protein complementarity principle, by polysaccharide component in edible fungi
Compound with selenium-enriched protein peptide in rice, be not only effectively utilized Testa oryzae resource but also serve the effect of strengthening selenium element.In rice, rely
Propylhomoserin (Lys) is the first limiting amino acid, and in edible fungi, in polysaccharide component, lysine content is than abundant, therefore at Testa oryzae egg
Polysaccharide component in edible fungi is added so that it is nutrition is more comprehensively in white；And also have that energy consumption is low, single-stage separation efficiency high, equipment letter
The advantage such as single, pollution-free.
Embodiment 2
With embodiment 1 except that, described preparation includes the edible fungi polysaccharide powder of 55wt% and the rice selenium-enriched protein of 45 wt%
Powder.Described preparation is tablet or pill；
Addition deionized water is mixed under power is 680W by step A2 with DCTZ-2000 multipurpose thermostatic ultrasonic extractor
After the ultrasonic 15min of solution.
Embodiment 3
With embodiment 1 except that, described preparation includes the edible fungi polysaccharide powder of 70 wt% and the rice selenium-enriched protein of 30wt%
Powder.Described preparation is oral liquid.
In step A2 with DCTZ-2000 multipurpose thermostatic ultrasonic extractor under power is 680W to add deionized water
The ultrasonic 20min of mixed solution.
Schematically being described the present invention and embodiment thereof above, this description does not has restricted, actual knot
Structure is not limited thereto.So, if those of ordinary skill in the art is enlightened by it, without departing from the invention objective
In the case of, design the frame mode similar to this technical scheme and embodiment without creative, all should belong to the present invention's
Protection domain.
Claims (10)
1. an edible fungi polysaccharide and rice rich in selenium protein peptide complex preparation, it is characterised in that described preparation includes 55～70wt%
Edible fungi polysaccharide powder and the rice egg rich in selenium white lead of 30～45wt%.
A kind of edible fungi polysaccharide the most according to claim 1 and rice rich in selenium protein peptide complex preparation, it is characterised in that described
Preparation includes the edible fungi polysaccharide powder of 65wt% and the rice egg rich in selenium white lead of 35wt%.
A kind of edible fungi polysaccharide the most according to claim 1 and rice rich in selenium protein peptide complex preparation, it is characterised in that described
The purity of edible fungi polysaccharide powder and rice egg rich in selenium white lead is all not less than 85%.
A kind of edible fungi polysaccharide the most according to claim 1 and rice rich in selenium protein peptide complex preparation, it is characterised in that described
Edible fungi includes Ganoderma, Lentinus Edodes, Hericium erinaceus (Bull. Ex Fr.) Pers., Coriolous Dersicolor (Fr.) Quel, Flammulina velutiper (Fr.) Sing, Volvariella volvacea (Bull.Ex Franch.) Singer．, Agaricus bisporus, Auricularia, Tremella and Pleurotus eryngii.
A kind of edible fungi polysaccharide the most according to claim 1 and rice rich in selenium protein peptide complex preparation, it is characterised in that described
Preparation is tablet, pill, capsule or oral liquid.
A kind of edible fungi polysaccharide the most according to claim 1 and rice rich in selenium protein peptide complex preparation, it is characterised in that described
Preparation is capsule.
7., according to a kind of edible fungi polysaccharide described in claim 6 and the preparation method of rice rich in selenium protein peptide complex preparation, it is special
Levy and be, including the supersound extraction of edible fungi polysaccharide, the preparation of rice selenium-enriched protein and will prepare according to above-mentioned ratio
Edible fungi polysaccharide powder, rice egg rich in selenium white lead carry out nutrition and compound, fill plate with accurate capsule and capsule be filled with, collect
Capsule obtains edible fungi polysaccharide and rice selenium-enriched protein nutrition compounds capsule finished product.
A kind of edible fungi polysaccharide the most according to claim 7 and rice rich in selenium protein peptide complex preparation, it is characterised in that described
The supersound extraction of edible fungi polysaccharide comprises the steps:
A1. by fresh food bacterium dry in the sun to after the most half-dried, it is put in baking oven air blast under the conditions of 60 DEG C and dries, then pulverize through 30B
Machine is pulverized, and double-roll crusher crushes, and 80 mesh sieves divide, and jet mill is pulverized, and 300 mesh sieves divide, and prepares edible fungi superfine powder, puts
Standby in-20 DEG C；
A2. weigh described edible fungi superfine powder, add 85% appropriate ethanol and stand 2h at room temperature, with remove partial pigment and
Impurity, adds 420mL deionized water according still further to ratio of water to material 28mL/g and fully mixes, carry with DCTZ-2000 multipurpose thermostatic ultrasonic
The machine that takes under power is 680W ultrasonic 15～20min, obtains lixiviating solution；
A3. take step A2 gained lixiviating solution centrifugal 15min under the speed of 5000r/min, take supernatant i.e. supernatant one, described on
The Sevag reagent method Deproteinization that clear liquid one uses n-butyl alcohol and chloroform volume ratio to be 1:4, and under the conditions of 8000r/min
Centrifugal 10min, takes supernatant i.e. supernatant two；
A4. being put by described supernatant two and be evaporated under the conditions of 50 DEG C in Rotary Evaporators in right amount, concentrated solution adds 4 times of volumes
Dehydrated alcohol is refrigerator precipitation 12h under the conditions of 4 DEG C；
A5. take step A4 products therefrom and be centrifuged 15min taking precipitate in 5000r/min, and precipitate puts 60 DEG C of oven for drying, i.e.
The edible fungi polysaccharide of available ultrasonic extraction.
A kind of edible fungi polysaccharide the most according to claim 8 and rice rich in selenium protein peptide complex preparation, it is characterised in that step
Rotary Evaporators described in A4 is N-1100EYELA type Rotary Evaporators.
A kind of edible fungi polysaccharide the most according to claim 7 and rice rich in selenium protein peptide complex preparation, it is characterised in that institute
The preparation stating rice selenium-enriched protein comprises the steps:
B1. weighing 5g rice dry powder in 400ml beaker, add 250ml, 0.1M, pH value is the PBS buffer solution of 6.6-7.0,
Adding cellulase with pectase mass ratio is the mixed enzyme of 7:3, stirs, lucifuge in the water-bath of 30-40 DEG C of condition
Extract 8 hours；
B2. by the step B1 products therefrom filtered through gauze of 100-200 mesh, collect clear liquid one, progressively add in described clear liquid one
Enter analytically pure solid (NH4)2SO4To final concentration of 20%；
B3. step B2 products therefrom is saltoutd 10-14 hour, and with the centrifugation 10min of 8000r/min, removes foreign protein,
Obtain clear liquid two；
B4. in described clear liquid two, it is gradually added analytically pure solid (NH4)2SO4To final concentration of 45%；
B5. step B4 products therefrom is saltoutd 12 hours, and the centrifugation 10min of 8000r/min, remove clear liquid, will precipitation
Dissolve, dialyse 24 hours with the bag filter flowing water that molecular weight is 3000, then dialyse 24 hours with deionized water, within every 4 hours, change one
Secondary water, by the solution constant volume in bag filter to 200ml, detection i.e. obtains rice selenium-enriched protein.
Priority Applications (1)
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| CN112438357A (en) * | 2019-09-02 | 2021-03-05 | 刘玉亭 | Selenium peptide polysaccharide protein composite functional solid beverage for conditioning sub-health and processing method thereof |
| CN114304599A (en) * | 2021-11-15 | 2022-04-12 | 安徽中志科技有限公司 | Selenium-rich edible fungus powder with light flavor, production process and application thereof |
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