KR20130055926A - Extracting method of highly purified fucoidan - Google Patents
Extracting method of highly purified fucoidan Download PDFInfo
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- KR20130055926A KR20130055926A KR1020110121604A KR20110121604A KR20130055926A KR 20130055926 A KR20130055926 A KR 20130055926A KR 1020110121604 A KR1020110121604 A KR 1020110121604A KR 20110121604 A KR20110121604 A KR 20110121604A KR 20130055926 A KR20130055926 A KR 20130055926A
- Authority
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- South Korea
- Prior art keywords
- seaweed
- fucoidan
- products
- high purity
- extracting
- Prior art date
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- 229920000855 Fucoidan Polymers 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 20
- 241001474374 Blennius Species 0.000 claims abstract description 29
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 29
- 239000005017 polysaccharide Substances 0.000 claims abstract description 29
- 239000006227 byproduct Substances 0.000 claims abstract description 23
- 102000004190 Enzymes Human genes 0.000 claims abstract description 5
- 108090000790 Enzymes Proteins 0.000 claims abstract description 5
- 238000000605 extraction Methods 0.000 claims abstract description 5
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 5
- 238000001035 drying Methods 0.000 claims abstract description 4
- 150000004676 glycans Chemical class 0.000 claims abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 5
- 235000015110 jellies Nutrition 0.000 claims description 4
- 239000008274 jelly Substances 0.000 claims description 4
- 239000004480 active ingredient Substances 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000000227 grinding Methods 0.000 claims description 2
- 241001512723 Ecklonia Species 0.000 claims 1
- 235000008935 nutritious Nutrition 0.000 claims 1
- 241001512722 Ecklonia cava Species 0.000 abstract description 6
- 238000004108 freeze drying Methods 0.000 abstract description 2
- 241000264279 Sargassum fusiforme Species 0.000 abstract 1
- 238000010298 pulverizing process Methods 0.000 abstract 1
- 150000004804 polysaccharides Chemical class 0.000 description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 241000195493 Cryptophyta Species 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 239000002994 raw material Substances 0.000 description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 4
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 241000199919 Phaeophyceae Species 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 229910052791 calcium Inorganic materials 0.000 description 4
- 239000011575 calcium Substances 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid group Chemical group S(O)(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 3
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 3
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 229920000615 alginic acid Polymers 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 150000002772 monosaccharides Chemical class 0.000 description 3
- HNSDLXPSAYFUHK-UHFFFAOYSA-N 1,4-bis(2-ethylhexyl) sulfosuccinate Chemical compound CCCCC(CC)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(CC)CCCC HNSDLXPSAYFUHK-UHFFFAOYSA-N 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 2
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 239000000783 alginic acid Substances 0.000 description 2
- 229960001126 alginic acid Drugs 0.000 description 2
- 150000004781 alginic acids Chemical class 0.000 description 2
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000019784 crude fat Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 229930182830 galactose Natural products 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 244000215068 Acacia senegal Species 0.000 description 1
- 235000006491 Acacia senegal Nutrition 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 206010010144 Completed suicide Diseases 0.000 description 1
- AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 229920001543 Laminarin Polymers 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- -1 Polysaccharide sulfate Chemical class 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- 241000206572 Rhodophyta Species 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical class [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000001594 aberrant effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003627 anti-cholesterol Effects 0.000 description 1
- 230000002429 anti-coagulating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 description 1
- 229910001626 barium chloride Inorganic materials 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 201000005917 gastric ulcer Diseases 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 229940097043 glucuronic acid Drugs 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000007603 infrared drying Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- DBTMGCOVALSLOR-VPNXCSTESA-N laminarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)OC1O[C@@H]1[C@@H](O)C(O[C@H]2[C@@H]([C@@H](CO)OC(O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-VPNXCSTESA-N 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000002964 rayon Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000015096 spirit Nutrition 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/60—Edible seaweed
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/50—Polysaccharides, gums
- A23V2250/51—Polysaccharide
Abstract
Description
본 발명은 해조류 부산물로부터 유용물질을 추출하는 방법에 관한 것으로, 보다 구체적으로 해조류 부산물로부터 고순도의 후코이단을 효율적으로 추출하는 방법에 관한 것이다.
The present invention relates to a method for extracting useful substances from seaweed by-products, and more particularly to a method for efficiently extracting high-purity fucoidan from seaweed by-products.
해조류는 60~95% 정도가 수분으로 이루어져 있고, 나머지 성분 중 탄수화물이 가장 많아 당질이 전체 물량 기준으로 거의 50%이상 된다. 탄수화물에 이어 회분이 많고 종류에 따라서는 40%나 되는 것도 있다. 이러한 해조류의 주요 성분인 다당류는 바깥층에서 미세한 섬유 상의 결정 구조인 세포벽 다당, 이를 덮고 있는 무정형 겔 상태의 점질다당, 세포 안에 있는 저장 다 당으로 세 가지로 나눌 수 있다. Algae are composed of about 60 to 95% of water, and carbohydrates are the most of the rest, so the sugar content is almost 50% or more based on the total amount. After carbohydrates, there are a lot of ash, depending on the type of 40%. Polysaccharides, the main components of these seaweeds, can be classified into three types: cell wall polysaccharide, which is a crystal structure of fine fibers on the outer layer, viscous polysaccharide covering an amorphous gel, and storage polysaccharide in the cell.
해조류는 염류의 농도가 높고 유속이 빠르며 수압이 높은 해수 속에서 살다 보니 이러한 생육환경에 적응하기 위해 다당류의 역할이 더욱 크다. 다 당으로 구성된 해조류의 세포벽은 육상 식물보다 두껍지만 유연하면서 탄성이 좋다. 더욱이 육상식물에는 없는 세포간 다 당(점질다당)은 해수 중의 이온을 선택적으로 흡수하고 교환하고 수분을 일정 수준으로 유지한다. 또한, 해조류의 저장 다당 중에는 육상식물에 없는 것들이 많이 들어 있을뿐더러 해조 다당류의 물리화학적 성질이나 구조도 육상 식물에 비해 복잡하다. Since algae live in seawater with high salt concentration, high flow rate, and high water pressure, polysaccharides play a greater role in adapting to this growing environment. The cell wall of algae composed of polysaccharides is thicker than land plants, but flexible and elastic. Moreover, intercellular polysaccharides (viscopolysaccharides), which are not found in terrestrial plants, selectively absorb and exchange ions in seawater and maintain moisture at a constant level. In addition, many of the algae storage polysaccharides are not present in land plants, and the physical and chemical properties and structures of the algae polysaccharides are more complicated than those of land plants.
해조류는 크게 녹조, 갈조, 홍조로 분류되며, 종류에 따라 그 구성성분은 조금씩 다르다. Algae are classified into green algae, brown algae, and red algae, and their constituents vary slightly depending on the type.
이러한 다당류의 유용성은 다음과 같다.The usefulness of such polysaccharides is as follows.
약리효과로는,In pharmacological effect,
1) 혈액응고방지, 혈중 중성지질 감소 : 해조 황산다당, 후코이단, 카라기난, 사라가산 1) Prevention of blood coagulation, reduction of neutral lipid in blood: Seaweed polysaccharide, fucoidan, carrageenan, saragasan
2) 항콜레스테롤효과 : 해조 산성다당 2) Anticholesterol effect: Seaweed acid polysaccharide
3) 항암효과 : 칼조류의 황산다당, 청각, 후코이단 3) Anticancer effect: Polysaccharide sulfate, hearing, fucoidan of algae
영양효과 : 가용성 식이섬유소 Nutritional Effect: Soluble Dietary Fiber
에너지자원 : 바이오매스 Energy Resources: Biomass
해조바이오테크놀로지 : 헤조 다당 분해효소 이용 프로토플라스트 제조 Seaweed biotechnology: Protoplasm production using hezopolysaccharide degrading enzyme
이중 갈조류는 수분과 다당 외 여러 무기질로 구성 되어 있으며 다당류 성분은 다음과 같다.Double brown algae are composed of water, polysaccharides and other minerals.
1) 세포벽다당 : β-D-Glc-(1→4)의 직쇄 글루칸 1) Cell wall polysaccharide: linear glucan of β-D-Glc- (1 → 4)
2) 점질다당 : 해조 특유 다당, 알긴산, 후코이단, sargassan 2) viscous polysaccharides: polysaccharide, alginic acid, fucoidan, sargassan
*알긴산 : Na-알기네이트 식품첨가물, 섬유(일진산레이온), 아이스크림, 치즈안정제, 의약용 거즈, 다이어트식품(Sr, Cd 체내 흡억제, 정장작용, 무칼로리) * Alginic acid: Na-alginate food additive, fiber (iljin acid rayon), ice cream, cheese stabilizer, medicinal gauze, diet food (Sr, Cd body absorption inhibitory, dressing action, calorie-free)
3)저장다당 : 라미나란 3) Storage polysaccharide: Laminaran
그 외 갈조류가 포함하고 있는 무기질 성분으로 칼슘, 칼륨, 마그네슘, 요오드, 철, 아연 등이 있으며 이는 제5의 영양소라고 알려질 정도로 인체 및 동물의 성장이나 각종 질병의 예방에 탁월한 효능이 있는 것으로 알려지고 있다.
In addition, minerals contained in brown algae include calcium, potassium, magnesium, iodine, iron, and zinc, which are known as fifth nutrients, and are known to have excellent effects on human and animal growth and prevention of various diseases. have.
해조는 여러 가지 유용물질을 포함하고 있으나, 이중 특히 주목할 것이 후코이단과 무기질 성분이며, 본 발명의 출원인이 활용하고자 하는 성분은 후코이단과 칼슘이며 그 내용 및 효능은 다음과 같다.Seaweed contains a variety of useful materials, of particular note is the fucoidan and inorganic components, the components of the applicant of the present invention to utilize fucoidan and calcium and the content and efficacy are as follows.
해조류에 포함되어있는 후코이단의 분자량은 평균 MW 20KDa이며 후코스(Fucose)라는 기본당과 황산기가 결합되어 있다. 황산화 후코이단에서 생기는 F-후코이단과 20% 정도의 글루쿠론산를 포함한 U-후코이단 두 종류가 있다. 기능성 식품의 기초원료, 식이보조제, 첨가제,특효약으로 알려지고 있다. 후코이단은 갈조류에 유연성을 부여하여 격렬한 조수의 흐름으로부터 보호되도록 한다.The average molecular weight of fucoidan in seaweed is MW 20KDa, which combines a basic sugar called fucose and a sulfate group. There are two types of F-fucoidan from sulfated fucoidan and U-fucoidan containing about 20% glucuronic acid. It is known as a basic raw material, dietary supplement, additive, and special medicine of functional food. Fucoidan adds flexibility to brown algae, protecting it from the intensive tidal currents.
후코이단은 콜레스테롤의 배설을 도와 혈중 콜레스테롤 수치를 낮추며 혈관 질환을 예방할 수 있고 비만이 성인병으로 연결되는 것을 미리 예방하여 준다. 또한, 일본과 미국의 연구에서는 후코이단이 혈액응고방지작용, 항종양작용, 위궤양 치료 촉진작용, 항균작용, 혈압상승억제작용, 간세포증식인자(HGF)생산유도, 혈당상승억제작용, 항알레르기작용, 항바이러스작용이 있다고 하였다. 특히 소화기계통 암 종류 치유에 70-80% 효과가 있는 것으로 보고된 바 있으며, 대부분의 암 치유에도 탁월한 효과가 있는 것으로 나타났다. F-후코이단은 임파종 세포줄기의 자살을 유도하며 토끼에서는 이상증식을 억제할 수 있다는 보고가 있다.
Fucoidan helps to excrete cholesterol, lower blood cholesterol levels, prevent vascular disease, and prevent obesity leading to adult disease. In addition, studies in Japan and the United States have shown that fucoidan has anticoagulant action, antitumor action, gastric ulcer stimulation effect, antibacterial action, blood pressure suppression action, hepatocellular growth factor (HGF) production induction, blood sugar elevation suppression action, antiallergic action, It is said to have antiviral action. In particular, it has been reported to be 70-80% effective in the treatment of cancers of the digestive system, and also excellent in healing most cancers. F-fucoidan has been reported to induce suicide of lymphoma stem cells and inhibit aberrant proliferation in rabbits.
본 발명은 값싼 원료인 톳 또는 감태를 1차 가공한 후 얻어진 부산물에서 고순도의 후코이단을 효율적인 방법으로 얻고, 이를 젤리 형태의 영양식품으로 제조하는 것을 목적으로 한다.
An object of the present invention is to obtain a high-purity fucoidan from the by-product obtained after the primary processing of the cheap raw materials or Ecklonia cava in an efficient manner, and to prepare it as a nutritional food in the form of jelly.
본 발명은 고순도 후코이단의 추출방법에 관한 것으로, 보다 구체적으로 해조류 가공 부산물을 건조하고 분쇄하는 단계; 상기 분쇄된 부산물을 효소를 이용하여 분해 및 추출하여 추출물을 수득하는 단계; 상기 추출물을 한외여과막을 이용하여 분자량별 분획물을 제조하는 단계; 상기 분획물에 주정을 첨가한 후 보관하는 단계; 및 침전된 다당류를 수거하여 동결건조하는 단계; 를 포함하는 고순도 후코이단의 추출방법에 관한 것이다.
The present invention relates to a method for extracting high purity fucoidan, and more specifically, drying and grinding seaweed processing by-products; Dissolving and extracting the pulverized byproduct using an enzyme to obtain an extract; Preparing fractions for each molecular weight of the extract using an ultrafiltration membrane; Adding alcohol to the fractions and storing the alcohol; And collecting and lyophilizing the precipitated polysaccharide; It relates to a high purity fucoidan extraction method comprising a.
본 발명에서 상기 해조류는 특별히 종류가 제한되는 것은 아니나, 톳 또는 감태인 것이 바람직할 수 있다.
In the present invention, the seaweed is not particularly limited in kind, it may be preferable that the seaweed or Ecklonia cava.
본 발명에서 상기 주정은 특별히 첨가 비율이 정해진 것은 아니나, 분획물 대비 2배의 비율로 첨가하는 것이 바람직할 수 있다.
In the present invention, the alcohol is not particularly determined in the addition ratio, it may be preferable to add at a ratio of twice the fraction.
본 발명에서 상기 침전된 다당류의 수거방법은 특별히 제한적인 것은 아니나, 10,000rpm에서 원심분리하여 수거하는 것이 바람직할 수 있다.
The method of collecting the precipitated polysaccharide in the present invention is not particularly limited, but may be preferably collected by centrifugation at 10,000 rpm.
본 발명의 다른 양태로는 상기 방법에 의해 추출된 고순도 후코이단을 유효성분으로 포함하는 젤리 형태의 영양식품이 될 수 있다.
Another embodiment of the present invention may be a nutritional food in the form of jelly containing high purity fucoidan extracted by the method as an active ingredient.
본 발명은 값싼 원료인 톳 또는 감태를 1차 가공한 후 얻어진 부산물에서 고순도의 후코이단을 막분리 방식을 통해 효율적으로 얻고, 이를 젤리 형태의 영양식품으로 제조할 수 있다.The present invention can efficiently obtain a high-purity fucoidan from the by-product obtained after the primary processing of the cheap raw material or Ecklonia cava by membrane separation method, it can be prepared as a nutritional food in the form of jelly.
본 발명에 의하면, 현재 국내에 소비되는 해조 칼슘 및 후코이단 원료의 대부분이 수입에 의존하고 있어 본 기술의 개발로 인하여 국내에 소비되는 원료의 일부분을 대체할 수 있을 것으로 기대된다.According to the present invention, since most of the seaweed calcium and fucoidan raw materials currently consumed in Korea depend on imports, it is expected that the development of the present technology may replace a part of raw materials consumed in Korea.
또한, 본 기술의 개발로 향후에 해조 칼슘 및 후코이단 원료제의 수출은 물론 해조가공부산물을 이용한 라이센스의 수출효과도 있을 것으로 기대된다. In addition, the development of this technology is expected to export seaweed calcium and fucoidan raw materials as well as export licenses using seaweed processed by-products.
그리고, 고령화 사회로 전환 되어가는 현대 사회에서의 건강 관련 관심의 증가는 꾸준히 상승할 것으로 예상되며, 이로 인하여 그 수요 또한 꾸준히 상승할 것으로 예상되는바 성장 잠재력 면에서 무한한 가능성이 있을 것으로 예상된다. In addition, the increase in health-related interest in the modern society, which is being converted into an aging society, is expected to increase steadily, and thus the demand is expected to rise steadily, and thus there is an infinite possibility in terms of growth potential.
아울러, 본 발명은 해조 가공부산물을 이용한 유용물질 회수 기술로서 추후 가공 부산물을 이용한 다양한 응용 기술 개발에 적용이 가능하다.
In addition, the present invention can be applied to the development of a variety of applications using the processing by-products as a useful material recovery technology using seaweed processing by-products.
이하, 본 발명을 실시예를 통해 상세히 설명하도록 한다. 하기 실시예는 본 발명을 설명하기 위한 일 예에 지나지 않으며, 이에 의해 본 발명의 범위가 제한되는 것은 아니다.
Hereinafter, the present invention will be described in detail with reference to examples. The following examples are only examples for describing the present invention, and the scope of the present invention is not limited thereto.
<실시예><Examples>
해조류 가공부산물(Seaweed Processing By-products ( 자숙액Self-payment )의 확보) Secure
채취한 자연산 해조류를(톳, 감태) 물 교환 방식으로 세척을 한 후 이물질을 제거하고 해조류를 30분간 스팀으로 찐 후의 추출된 해조 가공부산물(자숙액)을 확보하였다.
After washing the collected wild algae (톳, Ecklonia cava) by water exchange method, foreign substances were removed, and the seaweeds were steamed with steam for 30 minutes to obtain the extracted seaweed by-products (cooking solution).
해조류 가공부산물의 확보 및 추출Securing and Extracting Seaweed Processed By-Products
해조류(감태) 가공 부산물인 줄기 부분을 원적외선 건조공법을 이용하여 24시간 건조하고 분쇄한 후, 분쇄된 가공부산물을 효소를 이용하여 분해, 추출하였다.
The stem portion, which is a by-product of seaweed (Ecklonia cava) processing, was dried and ground for 24 hours using a far-infrared drying method, and then the milled by-products were decomposed and extracted using an enzyme.
해조 가공부산물의 이물질 제거Remove foreign substances from seaweed processing byproducts
해조 가공부산물의 이물질을 제거하기 위하여 원심 분리기 및 필터를 이용하여 잔사를 제거하였다.
In order to remove foreign matters of the seaweed by-products, the residue was removed using a centrifuge and a filter.
해조 가공부산물의 일반성분 및 수율 분석Analysis of General Components and Yields of Seaweed Processed By-Products
일반성분 분석방법은 AOAC법에 따라 수분은 105℃ 상압건조법, 조지방은 에테르를 이용한 Sohxlet법 (Sohxlet system 1046, TacatorAB, Sweden), 조단백질은 킬달자동분석장치 (KjeltecTM2300, Foss Co. Ltd., Denmark)를 사용하여 분석하며, 조회분은 550℃ 회화로에서 12시간 이상 태운 후 정량한다. 탄수화물은 전체 일반성분 함량을 100%로 하였을 때 수분, 조단백질, 조지방 및 조회분의 함량을 뺀 값으로 나타낸다. 총 폴리페놀 함량은 Folin-Denis법에 따라 시료 0.2mL에 증류수 1.8mL를 넣은 다음 Folin-ciocalteu's phenol reagent 0.2mL를 첨가하여 혼합한 후 3분간 실온에서 반응시키고, 반응이 끝나면 Na2CO3 포화용액 0.4mL를 가하여 혼합한 후 다시 증류수 1.4mL를 가한 다음 실온에서 1시간 반응시켜 UV/VIS spectrophotometer를 사용하여 725nm에서 흡광도를 측정하여 함량을 계산하였다.
General ingredient analysis method according to AOAC method, moisture is 105 ℃ atmospheric pressure drying method, Sohxlet method using ether with crude fat (Sohxlet system 1046, TacatorAB, Sweden), crude protein is KjeltecTM2300, Foss Co. Ltd., Denmark Analyze using, and the amount of inquiry is quantified after burning for more than 12 hours in 550 ℃ incinerator. Carbohydrate is expressed by subtracting the content of water, crude protein, crude fat and crude ash when the total general ingredient content is 100%. For total polyphenol content, add 1.8 mL of distilled water to 0.2 mL of sample according to the Folin-Denis method, add 0.2 mL of Folin-ciocalteu's phenol reagent, mix, and react at room temperature for 3 minutes. After completion of the reaction, 0.4 mL of saturated Na2CO3 solution was added. After adding and mixing, 1.4 mL of distilled water was added thereto, followed by reaction at room temperature for 1 hour, and the absorbance was measured at 725 nm using a UV / VIS spectrophotometer to calculate the content.
한외여과막을Ultrafiltration membrane 이용한 추출물의 분자량별 Molecular weight of extract used 분획물Fraction 제조 Produce
해조 가공부산물에서 해조 다당을 분리하기 위하여 milipore 한외여과막 장치를 이용하여 두 개의 막인 10 kDa, 30 KDa의 멤브레인을 이용해 3가지 분자량별 (5 KDa 이하, 5-30 KDa, 30 KDa 이상) 분획물을 제조한 후 동결건조를 하였다. 동결 건조된 분획물은 고농도로 녹였으며, 이 분획물에 주정을 1:2의 비율로 첨가하여 24h 저온에서 보관하였다. 침전된 다당류는 10,000rpm에서 원심분리하여 침전된 다당류를 수거하여 동결 건조한 후 단당 구성 성분을 분석하였다.
In order to separate seaweed polysaccharides from seaweed by-products, fractions of three molecular weights (5 KDa or less, 5-30 KDa, and 30 KDa or more) were prepared using two membranes, 10 kDa and 30 KDa, using a milipore ultrafiltration membrane device. After lyophilization. The freeze-dried fractions were dissolved in high concentrations and spirits were added to the fractions in a ratio of 1: 2 and stored at low temperature for 24h. Precipitated polysaccharide was centrifuged at 10,000 rpm to collect the precipitated polysaccharide, lyophilized and analyzed the monosaccharide components.
단당 구성 성분 분석 및 Monosaccharide component analysis and 황산기Sulfate 함량 분석 Content analysis
당 성분 분석을 위해 단당류인 fucose, Galactose, Mannose, Xylose의 함량을 측정하였다. 정제된 해조 다당을 2 M의 trifluoroacetic acid에 넣어 100℃에서 4 시간 동안 산 가수 분해 시켰다. 용매를 날린 후, 증류수를 넣어 다시 증류수를 제거하는 과정을 2~3회 반복하였다. 분석시 사용된 시스템은 HPAEC-PAD (Dionex, USA)이며 당 분석용 컬럼인 CarboPacTM PA1 (Dionex, USA)을 사용하였다. 18 mM NaOH를 이동상 용매로 사용하여 유속 1 ㎖/min 로 흘려주고, 산 가수 분해된 샘플을 10 ㎕ 넣어 분석하였다. 당 성분은 분석에 이용된 당 표준물질 (Fucose, Galactose, Mannose, Xylose)의 머무른 시간과 상호 비교하여 분석하였다. 황산기 함량을 확인하기 위해 BaCl2/gelation 방법을 이용해 황산기를 측정하였다. 구조의 변형을 가져와서 단순한 구조로 만들어주기 위해 sulfated polysaccharide 0.5 g/㎖, 1 g/㎖를 각각 4 M의 HCl을 가지고 100℃에서 약 2시간동안 가수분해시킨 후, 1 ㎖의 sulfated polysaccharide을 6.25% nitric acid 1 ㎖, 0.5% gum acacia를 가지는 8.7 N의 아세트산 0.5 ㎖, Barium chloride 5 g과 10분간 상온에서 반응시켜, 440 nm에서 흡광도를 측정하였다. 황산기 함량은 표준물질인 Ammonium sulfate를 이용하여 얻은 표준곡선식에 대입하여 측정하였다.
For the sugar component analysis, the contents of the monosaccharides fucose, Galactose, Mannose, and Xylose were measured. Purified seaweed polysaccharide was added to 2M trifluoroacetic acid and acid hydrolyzed at 100 ° C for 4 hours. After the solvent was blown, the process of removing distilled water by adding distilled water was repeated two or three times. The system used in the analysis was HPAEC-PAD (Dionex, USA) and used CarboPacTM PA1 (Dionex, USA), a sugar analysis column. 18 mM NaOH was used as the mobile phase solvent and flowed at a flow rate of 1 ml / min, and 10 µl of the acid hydrolyzed sample was analyzed. The sugar component was analyzed by comparing the retention time of sugar standards (Fucose, Galactose, Mannose, Xylose) used in the analysis. In order to confirm the sulfuric acid group content, the sulfuric acid group was measured using BaCl 2 / gelation method. 0.5 g / mL of sulfated polysaccharide and 1 g / mL were hydrolyzed at 100 ° C. for about 2 hours in order to bring about a simple structure and to make a simple structure, and then 1 mL of sulfated polysaccharide was 6.25. The absorbance was measured at 440 nm by reacting 1 mL of nitric acid, 0.5 mL of 8.7 N acetic acid with 0.5% gum acacia, and 5 g of barium chloride at room temperature for 10 minutes. Sulfuric acid content was measured by substituting a standard curve obtained using Ammonium sulfate as a standard.
이상에 설명한 바와 같이, 본 발명이 속하는 기술분야의 당업자는 본 발명이 그 기술적 사상이나 필수적 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 본 발명의 범위는 상기의 상세한 설명보다는 후술할 특허청구범위에 의하여 나타내어지며, 특허청구범위의 의미 및 범위 그리고 그 등가개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.As described above, those skilled in the art will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. It will be understood by those skilled in the art that the present invention may be embodied in many other specific forms without departing from the spirit or scope of the invention as defined by the appended claims and their equivalents. .
Claims (5)
상기 분쇄된 부산물을 효소를 이용하여 분해 및 추출하여 추출물을 수득하는 단계;
상기 추출물을 한외여과막을 이용하여 분자량별 분획물을 제조하는 단계;
상기 분획물에 주정을 첨가한 후 보관하는 단계; 및
침전된 다당류를 수거하여 동결건조하는 단계; 를 포함하는 고순도 후코이단의 추출 방법.
Drying and grinding seaweed processing byproducts;
Dissolving and extracting the pulverized byproduct using an enzyme to obtain an extract;
Preparing fractions for each molecular weight of the extract using an ultrafiltration membrane;
Adding alcohol to the fractions and storing the alcohol; And
Collecting and lyophilizing the precipitated polysaccharide; Extraction method of high purity fucoidan comprising a.
상기 해조류는 톳 또는 감태인 것을 특징으로 하는 고순도 후코이단의 추출 방법.
The method of claim 1,
The seaweed is a method of extracting high purity fucoidan, characterized in that the 감 or Ecklonia.
상기 주정은 분획물 대비 2배의 비율로 첨가하는 것을 특징으로 하는 고순도 후코이단의 추출 방법.
The method of claim 1,
The ethanol is a method of extracting high purity fucoidan, characterized in that added to the ratio of twice the fraction.
상기 침전된 다당류는 10,000rpm에서 원심분리하여 수거하는 것을 특징으로 하는 고순도 후코이단의 추출 방법.
The method of claim 1,
The precipitated polysaccharide is a high purity fucoidan extraction method characterized in that the collection by centrifugation at 10,000rpm.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101860137B1 (en) * | 2018-01-03 | 2018-05-21 | 한국해양바이오클러스터 (주) | A method for preparing a gentle extract mixture containing diecor and fucoidan as active ingredients of menthol |
| KR101866906B1 (en) | 2017-04-05 | 2018-06-12 | 한남대학교 산학협력단 | a extraction method of fucoidan |
| KR101973806B1 (en) | 2017-12-29 | 2019-04-29 | 한남대학교 산학협력단 | Fabrication and Characterization of Core-shell Nanofiber(PCL@Gelatin/Fucoidan) by Electrospinning |
| KR20190081659A (en) | 2017-12-29 | 2019-07-09 | 한남대학교 산학협력단 | Preparation of Composite Nanoparticles(Fucoidan/Chitosan/Pluronic) Using Synthesized Methacrylated Fucoidan |
| KR20220029834A (en) | 2020-08-28 | 2022-03-10 | 한남대학교 산학협력단 | A method manufacturing composite nanoparticle comprising vitamin C and fucoidan |
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2011
- 2011-11-21 KR KR1020110121604A patent/KR20130055926A/en not_active Application Discontinuation
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101866906B1 (en) | 2017-04-05 | 2018-06-12 | 한남대학교 산학협력단 | a extraction method of fucoidan |
| KR101973806B1 (en) | 2017-12-29 | 2019-04-29 | 한남대학교 산학협력단 | Fabrication and Characterization of Core-shell Nanofiber(PCL@Gelatin/Fucoidan) by Electrospinning |
| KR20190081659A (en) | 2017-12-29 | 2019-07-09 | 한남대학교 산학협력단 | Preparation of Composite Nanoparticles(Fucoidan/Chitosan/Pluronic) Using Synthesized Methacrylated Fucoidan |
| KR101860137B1 (en) * | 2018-01-03 | 2018-05-21 | 한국해양바이오클러스터 (주) | A method for preparing a gentle extract mixture containing diecor and fucoidan as active ingredients of menthol |
| KR20220029834A (en) | 2020-08-28 | 2022-03-10 | 한남대학교 산학협력단 | A method manufacturing composite nanoparticle comprising vitamin C and fucoidan |
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