Technical background
Rhizoma Gastrodiae is as famous and precious Chinese medicine raw material, have sedation, anticonvulsant action, analgesic activity, antiinflammatory action, delaying cell aging effect and to the effect of higher nervous center and cardiovascular system, confirmed by the modern pharmacology institute, effect is remarkable, in modern medical service is clinical, obtained using more widely, as products such as gastrodia buccal tablet, gastrodia liquor, gastrodia royal jelly, gastrodia capsules.But, the raw material that these products utilized depends on traditional Rhizoma Gastrodiae raw material processing technique more, this technology comprise Rhizoma Gastrodiae gather after through dry 4-5 days of washing, steaming or the well-done heart, shaping, coal fire, what have also wants sulphuring, to guarantee the storage and the color and luster of raw material.
Along with the restriction of food and drug safety aspect to strict control use sulfide in the raw material processing procedure, traditional Rhizoma Gastrodiae processing technique is under suspicion.Especially the Rhizoma Gastrodiae crude polysaccharides that carries out based on different efficacies and the method for separating and preparing of Rhizoma Gastrodiae alcohol soluble substance, report not also at present, the method for separating and preparing that for this reason with the Rhizoma Gastrodiae is the Rhizoma Gastrodiae crude polysaccharides of raw material and Rhizoma Gastrodiae alcohol soluble substance are a kind of useful examinations of repaying.
Summary of the invention
But the objective of the invention is by the strong Technology of simple and feasible action row, the Rhizoma Gastrodiae crude polysaccharides and the two big active components separation preparations of Rhizoma Gastrodiae alcohol soluble substance of different physiologically actives in the Rhizoma Gastrodiae.Under the prerequisite that guarantees the Rhizoma Gastrodiae effect, have few, the easy absorption of consumption, convenient storage, reduce and pollute the advantage of (as sulfide etc.), guaranteed the performance of Rhizoma Gastrodiae function, improved the physiological function of Rhizoma Gastrodiae to human body.
The present invention is achieved in that
(1) Rhizoma Gastrodiae is cleaned the back and complete, be as the criterion with the saturating heart of Rhizoma Gastrodiae with microwave method or cooking process;
(2) will complete back Rhizoma Gastrodiae fragmentation, add the centrifugal filtration of water mill slurry, twice different modes, be tripod pendulum type batch centrifugal coarse filtration and tube centrifuge fine straining, clear liquid under agitation adds three times of 95% ethanol of volume ratio, quiescent setting Rhizoma Gastrodiae crude polysaccharides, filter and take out the Rhizoma Gastrodiae crude polysaccharides, in normal pressure or vacuum, dry under the temperature 50-70 ℃ of condition, the broken Rhizoma Gastrodiae crude polysaccharide powder that obtains;
(3) the Rhizoma Gastrodiae filtering residue carries out percolation with the pure water clear liquid of precipitation behind the polysaccharide and extracts, Rhizoma Gastrodiae alcohol soluble substance extracting parameter is: pure its concentration of alcohol of water clear liquid is 70%-75%, alcohol water clear liquid and Rhizoma Gastrodiae filtering residue weight ratio are 15-20,2-3 hour percolation time, percolation number of times 2 times, and extracting solution is through concentrated, dry down, the broken Rhizoma Gastrodiae alcohol soluble substance powder that obtains of vacuum condition.
The invention has the beneficial effects as follows: but the Technology by a kind of simple use, technical feasibility industrializing implementation is separated into two kinds of components with the Rhizoma Gastrodiae extracts active ingredients.Its advantage is 1, avoid the pollution of Rhizoma Gastrodiae sulphur compound in drying process.2, two of separation and Extraction kinds of components can for the different curative effects of Rhizoma Gastrodiae, not same-action health food or Chinese patent medicine is single, the prescription goods provide basic material, under the prerequisite that guarantees the Rhizoma Gastrodiae effect, have that consumption is few, the advantage of easy absorption, guaranteed the performance of Rhizoma Gastrodiae function, improved the physiological function of Rhizoma Gastrodiae human body.3, complete to the extracts active ingredients of Rhizoma Gastrodiae raw material, reduce the wasting of resources.
Specific embodiment
Embodiment one
Rhizoma Gastrodiae 60kg is cleaned the back complete, be as the criterion with the saturating heart of Rhizoma Gastrodiae with microwave method;
With the back Rhizoma Gastrodiae fragmentation that completes, add the centrifugal filtration of water mill slurry, twice different modes, be tripod pendulum type batch centrifugal coarse filtration and tube centrifuge fine straining, clear liquid under agitation adds three times of 95% ethanol of volume ratio, quiescent setting Rhizoma Gastrodiae crude polysaccharides, filter and take out the Rhizoma Gastrodiae crude polysaccharides, dry under normal pressure, 50 ℃ of conditions of temperature, the broken Rhizoma Gastrodiae crude polysaccharide powder that obtains;
The Rhizoma Gastrodiae filtering residue carries out percolation with the pure water clear liquid of precipitation behind the polysaccharide and extracts, Rhizoma Gastrodiae alcohol soluble substance extracting parameter is: pure its concentration of alcohol of water clear liquid is 70%, alcohol water clear liquid and Rhizoma Gastrodiae filtering residue weight ratio are 15,2 hours percolation time, percolation number of times 2 times, extracting solution through concentrating, dry down, the broken acquisition Rhizoma Gastrodiae of vacuum condition alcohol soluble substance powder.
Embodiment two
Rhizoma Gastrodiae is cleaned the back complete, be as the criterion with the saturating heart of Rhizoma Gastrodiae with cooking process;
With the back Rhizoma Gastrodiae fragmentation that completes, add the centrifugal filtration of water mill slurry, twice different modes, be tripod pendulum type batch centrifugal coarse filtration and tube centrifuge fine straining, clear liquid under agitation adds three times of 95% ethanol of volume ratio, quiescent setting Rhizoma Gastrodiae crude polysaccharides, filter and take out the Rhizoma Gastrodiae crude polysaccharides, dry under vacuum, 65 ℃ of conditions of temperature, the broken Rhizoma Gastrodiae crude polysaccharide powder that obtains;
The Rhizoma Gastrodiae filtering residue carries out percolation with the pure water clear liquid of precipitation behind the polysaccharide and extracts, Rhizoma Gastrodiae alcohol soluble substance extracting parameter is: pure its concentration of alcohol of water clear liquid is 75%, alcohol water clear liquid and Rhizoma Gastrodiae filtering residue weight ratio 20,3 hours percolation time, percolation number of times 2 times, extracting solution is through concentrated, dry down, the broken Rhizoma Gastrodiae alcohol soluble substance powder that obtains of vacuum condition.
Embodiment three
Rhizoma Gastrodiae is cleaned the back complete, be as the criterion with the saturating heart of Rhizoma Gastrodiae with microwave method;
With the back Rhizoma Gastrodiae fragmentation that completes, add the centrifugal filtration of water mill slurry, twice different modes, be tripod pendulum type batch centrifugal coarse filtration and tube centrifuge fine straining, clear liquid under agitation adds three times of 95% ethanol of volume ratio, quiescent setting Rhizoma Gastrodiae crude polysaccharides, filter and take out the Rhizoma Gastrodiae crude polysaccharides, dry under vacuum, 70 ℃ of conditions of temperature, the broken Rhizoma Gastrodiae crude polysaccharide powder that obtains;
The Rhizoma Gastrodiae filtering residue carries out percolation with the pure water clear liquid of precipitation behind the polysaccharide and extracts, Rhizoma Gastrodiae alcohol soluble substance extracting parameter is: pure its concentration of alcohol of water clear liquid is 72%, alcohol water clear liquid and Rhizoma Gastrodiae filtering residue weight ratio 18,2.6 hours percolation time, percolation number of times 2 times, extracting solution is through concentrated, dry down, the broken Rhizoma Gastrodiae alcohol soluble substance powder that obtains of vacuum condition.
Embodiment four
Rhizoma Gastrodiae is cleaned the back complete, be as the criterion with the saturating heart of Rhizoma Gastrodiae with cooking process;
With the back Rhizoma Gastrodiae fragmentation that completes, add the centrifugal filtration of water mill slurry, twice different modes, be tripod pendulum type batch centrifugal coarse filtration and tube centrifuge fine straining, clear liquid under agitation adds three times of 95% ethanol of volume ratio, quiescent setting Rhizoma Gastrodiae crude polysaccharides, filter and take out the Rhizoma Gastrodiae crude polysaccharides, dry under normal pressure, 65 ℃ of conditions of temperature, the broken Rhizoma Gastrodiae crude polysaccharide powder that obtains;
The Rhizoma Gastrodiae filtering residue carries out percolation with the pure water clear liquid of precipitation behind the polysaccharide and extracts, Rhizoma Gastrodiae alcohol soluble substance extracting parameter is: pure its concentration of alcohol of water clear liquid is 74%, alcohol water clear liquid and Rhizoma Gastrodiae filtering residue weight ratio 16,2.8 hours percolation time, percolation number of times 2 times, extracting solution is through concentrated, dry down, the broken Rhizoma Gastrodiae alcohol soluble substance powder that obtains of vacuum condition.