CN101869587A - Method for extracting anti-gastric cancer active component from water chestnut shells - Google Patents
Method for extracting anti-gastric cancer active component from water chestnut shells Download PDFInfo
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- CN101869587A CN101869587A CN 201010213203 CN201010213203A CN101869587A CN 101869587 A CN101869587 A CN 101869587A CN 201010213203 CN201010213203 CN 201010213203 CN 201010213203 A CN201010213203 A CN 201010213203A CN 101869587 A CN101869587 A CN 101869587A
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- gastric cancer
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- pericarpium trapae
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Abstract
The invention discloses a method for extracting an anti-gastric cancer active component from water chestnut shells. The method sequentially includes the following steps that: (1) water chestnut shell powder is added with buffer solution with a pH value being equal to 2 and pepsin, kept still for extraction under 37DEG C of water bath and centrifugated; (2) filter residue obtained in step 1 is added with buffer solution with a pH value being equal to 8 and trypsin, kept still for extraction under 37DEG C of water bath and centrifugated; and (3) supernate obtained in step 2 is centrifugated at 12000 to 14000 rotations per minute for 25 to 35min, and after centrifugation, the obtained liquid is lyophilized, so that the anti-gastric cancer active component is obtained. The anti-gastric cancer active component extracted by adopting the method is characterized by high activity.
Description
Technical field
The present invention relates to a kind of method of extracting anti-gastric cancer active component in the Pericarpium Trapae.
Background technology
Pedicellus et Pericarpium Trapae (water chestnut) originates in the Europe and the warm area in Asia, is annual climing property aquatic herbaceous plant, has the cultivation history in more than 3000 year in China.The cultivated area of China's Pedicellus et Pericarpium Trapae is about 40000 hectares at present, about 240,000 tons of annual production.The Pedicellus et Pericarpium Trapae hatching rate is about 30%, and the bright Pericarpium Trapae of annual output can reach 71000～72000 tons according to statistics.A large amount of Pedicellus et Pericarpium Trapae shells are not effectively utilized, and not only contaminated environment also causes significant wastage.In recent years, Chinese scholar contains alkaloid, Flavonoid substances, polysaccharose substance and volatilization wet goods functional components by discovering in the Pericarpium Trapae, studies show that the water extract of Pericarpium Trapae has active anticancer.Adopt the anticancer active constituent in the modern technologies high efficiency extraction Pedicellus et Pericarpium Trapae shell, can solve of the pollution of discarded Pedicellus et Pericarpium Trapae shell environment, and to improving agricultural byproducts added value, the very important realistic meaning of having increased farmers' income.
Be to study as index substance mostly about the report that from Pericarpium Trapae, extracts anti-gastric cancer active component at present with the vegetalization compound of routines such as flavonoid, polysaccharide, this type of research is nothing but the anti-gastric cancer activity research of single-activity material finally, for example the anti-gastric cancer activity of Pericarpium Trapae flavone.The anti-gastric cancer effect of research single-activity composition has had very long history, but does not have big breakthrough aspect anti-gastric cancer active, and Chinese medicine is particular about is compatibility, and the activity of usual hybridization is the strongest.Though experiment in vitro proves above-mentioned single-activity material external tumor-suppression activity is arranged all, but have a greatly reduced quality, generally have only former activated about 30% through active situation behind the gastral Digestion.
The application of Pedicellus et Pericarpium Trapae class plant semi-bionic extraction method does not at present have report, utilizes external stripped stomach cancer cell model not report as the research of anti-gastric cancer screening active ingredients yet.
Summary of the invention
The technical problem to be solved in the present invention provides the method for extracting anti-gastric cancer active component from Pericarpium Trapae of the active height of a kind of extract, concise production process.
In order to solve the problems of the technologies described above, the invention provides a kind of method of from Pericarpium Trapae, extracting anti-gastric cancer active component, may further comprise the steps successively:
1), with the Pericarpium Trapae powder as raw material, in raw material, add the buffer of pH=2 and the pepsin that accounts for raw material gross weight 1%～10%, centrifugal then in 37 ℃ of static extraction 0.5～6h of water-bath, remove supernatant, filtering residue;
2), in step 1) gained filtering residue, add the buffer of pH=8 and account for the trypsin of raw material gross weight 1%～10%, centrifugal in 37 ℃ of static extraction 0.5～6h of water-bath, the supernatant of gained for have anti-gastric cancer active at the beginning of extracting solution;
3), with step 2) supernatant of gained carries out 12000～14000 rev/mins of centrifugal 25～35min, centrifugal back gained liquid is handled through lyophilizing, anti-gastric cancer active component.
As improvement of extracting the method for anti-gastric cancer active component from Pericarpium Trapae of the present invention: step 3) is: with the supernatant of gained prior to-20 ℃ of pre-freezes 0.5 hour, then in-50～-60 ℃ of lyophilizing 10～15 hours.
As the further improvements in methods of extracting anti-gastric cancer active component from Pericarpium Trapae of the present invention: Pericarpium Trapae is the South Lake Pericarpium Trapae.
As the further improvements in methods of extracting anti-gastric cancer active component from Pericarpium Trapae of the present invention: pepsic vigor is 3000-3500NF U/mg in the step 1), step 2) in tryptic vigor be 〉=250NF U/mg.
As the further improvements in methods of extracting anti-gastric cancer active component from Pericarpium Trapae of the present invention: the envelope-bulk to weight ratio of the buffer of pH=2 and raw material is in the step 1): the buffer of 10～50mL pH=2/g raw material; 3000 rev/mins of centrifugal 10min.
As the further improvements in methods of extracting anti-gastric cancer active component from Pericarpium Trapae of the present invention: the buffer of pH=8 and the envelope-bulk to weight ratio of raw material are step 2): the buffer of 10～50mL pH=8/g raw material; 3000 rev/mins of centrifugal 10min.
As the further improvements in methods of extracting anti-gastric cancer active component from Pericarpium Trapae of the present invention: centrifugal in the step 3) is: 13000 rev/mins of centrifugal 30min.
In extracting method of the present invention, the Pericarpium Trapae powder is that Pericarpium Trapae gets through pulverizing after the dried, the moisture content in the Pericarpium Trapae powder≤5%; As raw material, is in order to improve extraction efficiency with the Pericarpium Trapae powder.South Lake Pedicellus et Pericarpium Trapae described in the present invention is the acerous Pedicellus et Pericarpium Trapae of South Lake special product.
In extracting method of the present invention, glycine-hydrochloric acid buffer solution that the described pH=2 buffer of step 1) is pH=2, step 2) described pH=8 buffer is sodium hydrogen phosphate-sodium dihydrogen phosphate buffer of pH=8.Step 1) and step 2) in all taked 37 ℃ bionical water bath condition.
Employed pepsin belongs to the main digestive enzyme composition of digestive system stomach among the present invention, and employed trypsin belongs to the main digestive enzyme composition of digestive system small intestinal; Pepsin and trypsin are common commercial product, and for example, pepsin can be available from sky, lattice source profit Bioisystech Co., Ltd, and trypsin can be available from sky, lattice source profit Bioisystech Co., Ltd.
Performance for the anti-gastric cancer active component that detects gained of the present invention, earlier with the anti-gastric cancer active component of gained of the present invention solid-liquid ratio high purity water Hui Rong according to 0.05g raw material/ml high purity water, the aseptic filter membrane of 0.22 μ m (moisture film) filters then, the filtrate of filtering the back gained is used for stomach cancer cell handles, and the Detection and Extraction thing is to the inhibition activity of stomach cancer cell.Press down the cancer experiment confirm, survival has significant inhibition effect to the anti-gastric cancer active component of gained of the present invention to stomach cancer cell, has the potential value that is developed to natural anti-gastric cancer medicine.
The present invention has adopted and will suppress active to stomach cancer cell and optimize best extraction process for guiding combines with extracting method, and this method has been simulated oral medication digestive tract process and has been intended to extract through the digestive tract pair cell and still has the active composition of inhibition.
Semi-bionic extraction is that the biological activity with extract serves as that guiding simulation oral administration and medicine are transported through gastrointestinal tract, is a kind of novel more effective extracting method through the Chinese medicine preparation design of digestive tract administration.The extraction that half bionical extracting method is applied to the anti-gastric cancer active component of Pedicellus et Pericarpium Trapae can guarantee that not only extract has best anti-gastric cancer activity, and has verified that extract makes behind the oral class medicine thing through active situation behind the gastral Digestion.
Prior art adopts flavone or polysaccharide equipotential target substance about extraction of active ingredients in the Pericarpium Trapae and activity research more, has weakened the pharmacology of compatibility in the Chinese medicine to a certain extent, and the activity of extract is single relatively.Adopt method of the present invention that the anti-gastric cancer of extract is active in the guiding of extracting, can guarantee to extract meaningful property of result and target.Simultaneously, this extracts and adopts semi-bionic extraction, does not with an organic solvent wait, and belongs to safe and effective extracting mode.Also than being easier to control, required time only is 2～6 hours to extraction conditions of the present invention, and the time is shorter relatively.Therefore, the present invention is easier to suitability for industrialized production than previous methods, and cost is lower, and extraction effect is better.
The anti-gastric cancer active component detailed directions and the consumption of gained of the present invention are as follows: oral, every kg body weight is taken the anti-gastric cancer active component of 5～15mg.
The specific embodiment
Embodiment 1, a kind of method of from Pericarpium Trapae, extracting anti-gastric cancer active component, carry out following steps successively:
1), with 1g Pericarpium Trapae powder as raw material, glycine-the hydrochloric acid buffer solution of the pH=2 of adding 50ml and the pepsin (vigor is 3000-3500NF U/mg) of 0.01g in raw material, in the 37 ℃ of static extraction of water-bath 0.5h, all change in the centrifuge tube of 50mL then, 3000 leave heart 10min, remove supernatant, get filtering residue.
2), adding the sodium hydrogen phosphate-sodium dihydrogen phosphate buffer of 50mL pH=8 and the tryptic vigor of trypsin of 0.07g in step 1) gained filtering residue is 〉=250NF U/mg), in the 37 ℃ of static extraction of water-bath 70min, all change in the centrifuge tube of 50mL then, 3000 leave heart 10min, reclaim supernatant, supernatant is for having the active extracting solution just of anti-gastric cancer.
3), with step 2) supernatant of gained is placed in the 2mL centrifuge tube, 13000 leave heart 30min, centrifugal back gained liquid is handled through lyophilizing, specific as follows: that centrifugal back gained liquid is placed in the plate of diameter 10cm, the centrifugal back of each plate 5mL gained liquid, after half an hour, the lyophilizing outlet temperature is-54 ℃ in-20 ℃ of pre-freezes, and freeze-drying time is 15 hours; Must be as the lyophilized powder of anti-gastric cancer active component.
Collect all lyophilized powders, the high purity water that adds 20ml carries out Hui Rong, promptly carries out Hui Rong according to the concentration of every milliliter of high purity water of 0.05g Pericarpium Trapae powder (raw material); Get anti-gastric cancer active component solution.
The anti-gastric cancer active component solution of above-mentioned experimental example 1 gained is filtered through the aseptic filter membrane of 0.22 μ m (moisture film), and gained filtrate is used for stomach cancer cell (SGC7901) handles, and surveys the survival rate of stomach cancer cell with mtt assay.This anti-gastric cancer active component is 0.29 to the suppression ratio of stomach cancer cell as a result, has the effect that suppresses stomach cancer cell propagation.
Embodiment 2, a kind of method of from Pericarpium Trapae, extracting anti-gastric cancer active component, carry out following steps successively:
1), with 3g Pericarpium Trapae powder as raw material, glycine-the hydrochloric acid buffer solution of the pH=2 of adding 30ml and the pepsin (vigor is 3000-3500NF U/mg) of 0.06g in raw material, in the 37 ℃ of static extraction of water-bath 1h, all change in the centrifuge tube of 50mL then, 3000 leave heart 10min, remove supernatant, get filtering residue.
2), adding the sodium hydrogen phosphate-sodium dihydrogen phosphate buffer of 30mL pH=8 and the tryptic vigor of trypsin of 0.12g in step 1) gained filtering residue is 〉=250NF U/mg), in the 37 ℃ of static extraction of water-bath 70min, all change in the centrifuge tube of 50mL then, 3000 leave heart 10min, reclaim supernatant, supernatant is for having the active extracting solution just of anti-gastric cancer.
3), with step 2) supernatant of gained is placed in the 2mL centrifuge tube, 13000 leave heart 30min, centrifugal back gained liquid is handled through lyophilizing, specific as follows: that centrifugal back gained liquid is placed in the plate of diameter 10cm, the centrifugal back of each plate 5mL gained liquid, after half an hour, the lyophilizing outlet temperature is-54 ℃ in-20 ℃ of pre-freezes, and freeze-drying time is 15 hours; Must be as the lyophilized powder of anti-gastric cancer active component.
Collect all lyophilized powders, the high purity water that adds 60ml carries out Hui Rong, promptly carries out Hui Rong according to the concentration of every milliliter of high purity water of 0.05g Pericarpium Trapae powder (raw material); Get anti-gastric cancer active component solution.
The anti-gastric cancer active component solution of above-mentioned experimental example 2 gained is filtered through the aseptic filter membrane of 0.22 μ m (moisture film), and gained filtrate is used for stomach cancer cell handles, and surveys the survival rate of stomach cancer cell with mtt assay.This anti-gastric cancer active component is 0.36 to the suppression ratio of stomach cancer cell as a result, has the effect that suppresses stomach cancer cell propagation.
Embodiment 3, a kind of method of from Pericarpium Trapae, extracting anti-gastric cancer active component, carry out following steps successively:
1), with 1g Pericarpium Trapae powder as raw material, glycine-the hydrochloric acid buffer solution of the pH=2 of adding 40ml and the pepsin (vigor is 3000-3500NF U/mg) of 0.1g in raw material, in the 37 ℃ of static extraction of water-bath 1.5h, all change in the centrifuge tube of 50mL then, 3000 leave heart 10min, remove supernatant, get filtering residue.
2), adding the sodium hydrogen phosphate-sodium dihydrogen phosphate buffer of 40mL pH=8 and the tryptic vigor of trypsin of 0.05g in step 1) gained filtering residue is 〉=250NF U/mg), in the 37 ℃ of static extraction of water-bath 1h, all change in the centrifuge tube of 50mL then, 3000 leave heart 10min, reclaim supernatant, supernatant is for having the active extracting solution just of anti-gastric cancer.
3), with step 2) supernatant of gained is placed in the 2mL centrifuge tube, 13000 leave heart 30min, centrifugal back gained liquid is handled through lyophilizing, specific as follows: that centrifugal back gained liquid is placed in the plate of diameter 10cm, the centrifugal back of each plate 5mL gained liquid, after half an hour, the lyophilizing outlet temperature is-54 ℃ in-20 ℃ of pre-freezes, and freeze-drying time is 15 hours; Must be as the lyophilized powder of anti-gastric cancer active component.
Collect all lyophilized powders, the high purity water that adds 20ml carries out Hui Rong, promptly carries out Hui Rong according to the concentration of every milliliter of high purity water of 0.05g Pericarpium Trapae powder (raw material); Get anti-gastric cancer active component solution.
The anti-gastric cancer active component solution of above-mentioned experimental example 3 gained is filtered through the aseptic filter membrane of 0.22 μ m (moisture film), and gained filtrate is used for stomach cancer cell handles, and surveys the survival rate of stomach cancer cell with mtt assay.This anti-gastric cancer active component is 0.37 to the suppression ratio of stomach cancer cell as a result, has the effect that suppresses stomach cancer cell propagation.
At last, it is also to be noted that what more than enumerate only is several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (7)
1. method of extracting anti-gastric cancer active component from Pericarpium Trapae is characterized in that may further comprise the steps successively:
1), with the Pericarpium Trapae powder as raw material, in raw material, add the buffer of pH=2 and the pepsin that accounts for raw material gross weight 1%～10%, centrifugal then in 37 ℃ of static extraction 0.5～6h of water-bath, remove supernatant, filtering residue;
2), in step 1) gained filtering residue, add the buffer of pH=8 and account for the trypsin of raw material gross weight 1%～10%, centrifugal in 37 ℃ of static extraction 0.5～6h of water-bath, the supernatant of gained for have anti-gastric cancer active at the beginning of extracting solution;
3), with step 2) supernatant of gained carries out 12000～14000 rev/mins of centrifugal 25～35min, centrifugal back gained liquid is handled through lyophilizing, anti-gastric cancer active component.
2. according to the described method of extracting anti-gastric cancer active component from Pericarpium Trapae of claim 1, it is characterized in that: described step 3) is: with the supernatant of gained prior to-20 ℃ of pre-freezes 0.5 hour, then in-50～-60 ℃ of lyophilizing 10～15 hours.
3. according to the described method of extracting anti-gastric cancer active component from Pericarpium Trapae of claim 2, it is characterized in that: described Pericarpium Trapae is the South Lake Pericarpium Trapae.
4. according to claim 2 or the 3 described methods of extracting anti-gastric cancer active component from Pericarpium Trapae, it is characterized in that: pepsic vigor is 3000-3500NF U/mg in the step 1), step 2) in tryptic vigor be 〉=250NF U/mg.
5. the method for from Pericarpium Trapae, extracting anti-gastric cancer active component according to claim 4, it is characterized in that: the envelope-bulk to weight ratio of the buffer of pH=2 and raw material is in the described step 1): the buffer of 10～50mL pH=2/g raw material; 3000 rev/mins of centrifugal 10min.
6. the method for extracting anti-gastric cancer active component from Pericarpium Trapae according to claim 5 is characterized in that: the buffer of pH=8 and the envelope-bulk to weight ratio of raw material are described step 2): the buffer of 10～50mL pH=8/g raw material; 3000 rev/mins of centrifugal 10min.
7. the method for extracting anti-gastric cancer active component from Pericarpium Trapae according to claim 6 is characterized in that: centrifugal in the described step 3) is: 13000 rev/mins of centrifugal 30min.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1557227A (en) * | 2004-01-30 | 2004-12-29 | �Ϻ���ͨ��ѧ | Anticancer health beverage using water chestnut shell and coix seed as raw material and its making method |
| CN101433564A (en) * | 2008-12-24 | 2009-05-20 | 吉林大学 | Use of water chestnut extract in preparing medicament for treating leukaemia |
-
2010
- 2010-06-29 CN CN2010102132030A patent/CN101869587B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1557227A (en) * | 2004-01-30 | 2004-12-29 | �Ϻ���ͨ��ѧ | Anticancer health beverage using water chestnut shell and coix seed as raw material and its making method |
| CN101433564A (en) * | 2008-12-24 | 2009-05-20 | 吉林大学 | Use of water chestnut extract in preparing medicament for treating leukaemia |
Non-Patent Citations (1)
| Title |
|---|
| 《白求恩医科大学学报》 20011231 牛凤兰等 菱壳水提物对胃癌细胞抑制作用的实验研究 495-497 1-7 第27卷, 第5期 2 * |
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| CN104666746A (en) * | 2013-11-30 | 2015-06-03 | 白杨 | Preparation method of pepsin treated ARR (Angelica sinensis, Radix paeoniae alba and Rehmannia) oral liquid |
| CN104666739A (en) * | 2013-11-30 | 2015-06-03 | 白杨 | Preparation method of pepsin-trypsin treated CMR (Cinnamon, Monkshood and Rehmannia) compound micro-ecological oral liquid |
| CN104666749A (en) * | 2013-11-30 | 2015-06-03 | 白杨 | Method for preparing pepsase processed Guifu rehmannia oral solution |
| CN104666747A (en) * | 2013-11-30 | 2015-06-03 | 白杨 | Preparation method of pepsin treated Qiwei Duqi oral liquid |
| CN104666810A (en) * | 2013-11-30 | 2015-06-03 | 白杨 | Preparation method of Zhibai Dihuang oral liquid treated by pepsin and trypsin |
| CN104666740A (en) * | 2013-11-30 | 2015-06-03 | 白杨 | Preparation method of pepsin and trypsin treated Liuwei Dihuang composite micro-ecological oral liquid |
| CN104666744A (en) * | 2013-11-30 | 2015-06-03 | 白杨 | Preparation method of Gouju Dihuang oral liquid treated by pepsin |
| CN104666751A (en) * | 2013-11-30 | 2015-06-03 | 白杨 | Preparation method of Qiwei Duqi oral liquid treated by pepsin and trypsin |
| CN104666860A (en) * | 2013-11-30 | 2015-06-03 | 白杨 | Preparation method of pepsin-trypsin treated RSR (Radix ophiopogonis, Schisandra chinensis and Rehmannia) compound micro-ecological oral liquid |
| CN104666737A (en) * | 2013-11-30 | 2015-06-03 | 白杨 | Preparation method of pepsin and trypsin treated Liuwei Dihuang oral liquid |
| CN104666861A (en) * | 2013-11-30 | 2015-06-03 | 白杨 | Preparation method of baihe gujin (Chinese character) composite micro-ecological oral liquid treated by pepsase and trypsin |
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| CN101869587B (en) | 2011-09-28 |
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