CN104666737A - Preparation method of pepsin and trypsin treated Liuwei Dihuang oral liquid - Google Patents
Preparation method of pepsin and trypsin treated Liuwei Dihuang oral liquid Download PDFInfo
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- CN104666737A CN104666737A CN201310633530.5A CN201310633530A CN104666737A CN 104666737 A CN104666737 A CN 104666737A CN 201310633530 A CN201310633530 A CN 201310633530A CN 104666737 A CN104666737 A CN 104666737A
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Abstract
The invention relates to a preparation method of pepsin and trypsin treated Liuwei Dihuang oral liquid. The preparation method comprises the following steps: (1) crushing Liuwei Dihuang pill raw materials to prepare raw material powder, adding water, and mixing uniformly to prepare raw material serous fluid; (2) adding acid into the raw material serous fluid to adjust the pH value, and adding pepsin for treatment, and adjusting the pH value by using alkali, thereby preparing pepsin treated serous fluid; and (3) adding trypsin into the pepsin treated serous fluid for treatment, and adjusting the pH value to prepare the pepsin and trypsin treated Liuwei Dihuang oral liquid. The pepsin and trypsin treated Liuwei Dihuang oral liquid is prepared by the preparation method provided by the invention by decomposing the traditional Liuwei Dihuang pill raw materials by virtue of pepsin and trypsin, is relatively small in side effect, and is palatable in sweet and sour tastes; and by virtue of practical application, after being taken for a long time, the pepsin and trypsin treated Liuwei Dihuang oral liquid not only can achieve a treatment effect of Liuwei Dihuang pills but also can greatly reduce the side effects causing stomach cold and digestion obstruction.
Description
Technical field
The present invention relates to the preparation method of Liuweidihuang Oral Liquid of a kind of stomach, trypsin treatment, particularly a kind of method utilizing the classical LIUWEI DIHUANG WAN prescription of biotechnology process, belongs to biomedicine technical field.
Background technology
LIUWEI DIHUANG WAN stems from the JINGUI SHENQI WAN in the large physician's Zhang Zhongjing " Medical Treasures of the Golden Chamber " of the Eastern Han Dynasty, also be " shenqi pill ", " Rehmanniae Bolus of Eight Ingredients ", be originally eight taste Chinese crude drugs, afterwards to the Northern Song Dynasty, by national imperial physician assistant officer Qian Yi, former side is deducted Cortex Cinnamomi, Radix Aconiti Lateralis Preparata two taste Chinese medicine, defines by Radix Rehmanniae Preparata, Fructus Corni, Rhizoma Dioscoreae (three taste tonics), Poria, Rhizoma Alismatis, the LIUWEI DIHUANG WAN of Cortex Moutan (three taste cathartics) ingenious compatibility; Three mend and three to rush down, and mend and not dry.LIUWEI DIHUANG WAN, from Song dynasty Cheng Fang, continues more than 1000 year till now always, lasting through successive dynasties doctor and undergoing of patient, can be described as " volt cattle prescription changes, and within thousand, medicine does not wane ".
But because LIUWEI DIHUANG WAN belongs to sweet warm nature, the relatively medicine of nourishing YIN, cure mainly deficiency of the kidney yin, in its formula, cloudy soft medicine is more, so sometimes can antidigestive side effect after eating, therefore not only spleen and stomach function is weak, dyspepsia person is careful eats, and long-term taking can cause the malaise symptoms such as abdominal distention, loose stool, inappetence.
Chinese patent literature CN102920882A(application number 201110338130.2) disclose a kind of preparation method of concentrated pill of six ingredients with rehmannia, it is characterized in that comprising the steps: that (1) takes raw material by following weight portion: Radix Rehmanniae Preparata 120 parts, wine Fructus Corni 60 parts, Cortex Moutan 45 parts, Rhizoma Dioscoreae 60 parts, 45 parts, Poria, Rhizoma Alismatis 45 parts; (2) Cortex Moutan is dropped in extraction pot, extract paeonol with steam distillation; First open condensed water, open straight-through steam valve again, steam regulation pressure is at 0.1Mpa ~ 0.3Mpa, in tank, temperature remains on 100 ~ 110 DEG C, and pressure is ﹤ 0.05Mpa, and regulating condensate flow to make material pipe above crystallizer flow out paeonol distillate temperature is 40 DEG C ~ 60 DEG C, collect paeonol distillate, cold preservation, crystallize, collects paeonol crystal; (3) get Radix Rehmanniae Preparata 120 parts, wine Fructus Corni 20 parts, 45 parts, Poria, Rhizoma Alismatis 45 parts, adopt dynamic extraction mode, decoct with water 1 ~ 3 time, each 1 ~ 1.5 hour, decocting liquid filters, and merges, and is concentrated into the thick paste (55 ~ 60 DEG C of mother-in-law prunus mume (sieb.) sieb.et zucc. proportion measurements) that proportion is 1.35 ~ 1.40; (4) after Rhizoma Dioscoreae 60 parts and wine Fructus Corni 40 parts pulverized 80 mesh sieves, mixing, obtains fine powder, obtains sterilized powder after moist heat sterilization; (5) by the paeonol crystal of step (2) (3) (4) gained, thick paste, fine powder mixing, add pharmaceutically acceptable adjuvant, obtained wet ball, dry, polishing, to obtain final product.
Although the active ingredient in LIUWEI DIHUANG WAN can be extracted concentrated obtained concentrated pill of six ingredients with rehmannia by above-mentioned patent, the antidigestive side effect of LIUWEI DIHUANG WAN does not weaken.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, the preparation method of the Liuweidihuang Oral Liquid of a kind of stomach, trypsin treatment is provided.
Term explanation
Pepsic unit of activity (U): adopt the unit of activity that the detection method of Chinese Pharmacopoeia version formulation in 1977 detects.
Tryptic unit of activity (U): the unit of activity that the detection method adopting Chinese Pharmacopoeia nineteen ninety version to formulate detects.
Technical solution of the present invention is as follows:
A preparation method for the Liuweidihuang Oral Liquid of stomach, trypsin treatment, step is as follows:
(1) by the Six-element crude drug in LIUWEI DIHUANG WAN raw material after crushed, obtained material powder, then adds 200 ~ 600 grams of water and refined honey by every hectogram material powder, mix homogeneously, obtained raw slurry;
(2) in the obtained raw slurry of step (1), adding sour adjust pH is 1.5 ~ 2.2, add the pepsin of 20 ~ 60 unit of activity by every hectogram LIUWEI DIHUANG WAN raw material, temperature 36 ~ 38 DEG C, process 2 ~ 3 hours, adjusting PH with base value is 7.8 ~ 8.2, the serosity of obtained pepsin;
(3) in the serosity of the obtained pepsin of step (2), add the trypsin of 20 ~ 50 unit of activity by every hundred milliliters, temperature 36 ~ 38 DEG C, process 2 ~ 3 hours, acid adjust pH to 5.5 ~ 6, obtain the Liuweidihuang Oral Liquid of stomach, trypsin treatment.
Preferred according to the present invention, the LIUWEI DIHUANG WAN raw material of described step (1) is as follows, is weight portion:
Radix Rehmanniae Preparata 160 parts, Fructus Corni (processed) (wine Fructus Corni) 80 parts, Cortex Moutan 60 parts, Rhizoma Dioscoreae 80 parts, 60 parts, Poria, Rhizoma Alismatis 60 parts, refined honey 7 ~ 10 parts.
Preferred according to the present invention, the LIUWEI DIHUANG WAN raw material of described step (1) is as follows, is weight portion:
Radix Rehmanniae Preparata 160 parts, Fructus Corni (processed) (wine Fructus Corni) 80 parts, Cortex Moutan 60 parts, Rhizoma Dioscoreae 80 parts, 60 parts, Poria, Rhizoma Alismatis 60 parts, refined honey 16 ~ 22 parts.
Preferred according to the present invention, the acid in described step (2), (3) is hydrochloric acid.
Preferred according to the present invention, the alkali of described step (1) is sodium carbonate.
Beneficial effect
The present invention passes through traditional LIUWEI DIHUANG WAN raw material through stomach, trypsin resolution process, obtain the Liuweidihuang Oral Liquid of the less stomach of side effect, trypsin treatment, this product sour and sweet palatability, and through practical application, the Liuweidihuang Oral Liquid of this stomach of long-term taking, trypsin treatment can not only reach takes LIUWEI DIHUANG WAN therapeutic effect, and cause cold syndrome of the stomach, hinder digestion side effect also reduced greatly.
Detailed description of the invention
Below in conjunction with embodiment, technical scheme of the present invention is described further, but institute of the present invention protection domain is not limited thereto.
Embodiment 1
A preparation method for the Liuweidihuang Oral Liquid of stomach, trypsin treatment, step is as follows:
(1) by the Six-element crude drug in LIUWEI DIHUANG WAN raw material after crushed, obtained material powder, then adds 200 grams of water and refined honey by every hectogram material powder, mix homogeneously, obtained raw slurry;
(2) in the obtained raw slurry of step (1), adding hydrochloric acid adjust pH is 1.5, add the pepsin of 20 unit of activity by every hectogram LIUWEI DIHUANG WAN raw material, temperature 36 DEG C, process 3 hours, sodium carbonate adjust pH is 8, the serosity of obtained pepsin;
(3) in the serosity of the obtained pepsin of step (2), add the trypsin of 50 unit of activity by every hundred milliliters, temperature 37 DEG C, process 2 hours, hydrochloric acid adjust pH to 5.5, obtains the Liuweidihuang Oral Liquid of stomach, trypsin treatment.
The LIUWEI DIHUANG WAN raw material of described step (1) is as follows, is weight portion:
Radix Rehmanniae Preparata 160 parts, Fructus Corni (processed) (wine Fructus Corni) 80 parts, Cortex Moutan 60 parts, Rhizoma Dioscoreae 80 parts, 60 parts, Poria, Rhizoma Alismatis 60 parts, refined honey 7 parts.
Embodiment 2
A preparation method for the Liuweidihuang Oral Liquid of stomach, trypsin treatment, step is as follows:
(1) by the Six-element crude drug in LIUWEI DIHUANG WAN raw material after crushed, obtained material powder, then adds 600 grams of water and refined honey by every hectogram material powder, mix homogeneously, obtained raw slurry;
(2) in the obtained raw slurry of step (1), adding sour adjust pH is 2.2, add the pepsin of 60 unit of activity by every hectogram LIUWEI DIHUANG WAN raw material, temperature 38 DEG C, process 2 hours, sodium carbonate adjust pH is 7.8, the serosity of obtained pepsin;
(3) in the serosity of the obtained pepsin of step (2), add the trypsin of 20 unit of activity by every hundred milliliters, temperature 37 DEG C, process 3 hours, hydrochloric acid adjust pH to 6, obtains the Liuweidihuang Oral Liquid of stomach, trypsin treatment.
The LIUWEI DIHUANG WAN raw material of described step (1) is as follows, is weight portion:
Radix Rehmanniae Preparata 160 parts, Fructus Corni (processed) (wine Fructus Corni) 80 parts, Cortex Moutan 60 parts, Rhizoma Dioscoreae 80 parts, 60 parts, Poria, Rhizoma Alismatis 60 parts, refined honey 22 parts.
Test example 1
Yan, man, people from Changqing District, Jinan, soreness of the waist and knees, two skelasthenia, vertigo and tinnitus, insomnia and dreamful sleep, spleen and stomach function is normal.Take the Liuweidihuang Oral Liquid of the stomach described in embodiment 1, trypsin treatment, every day secondary, each 15 grams, take one month, symptom is obviously improved, and spleen and stomach function is normal.
Zhang, man, Licheng in Jinan city people, long-term taking LIUWEI DIHUANG WAN causes the symptom of cold syndrome of the stomach, abdominal distention, loose stool, inappetence.Take the Liuweidihuang Oral Liquid of the stomach described in embodiment 1, trypsin treatment, every day secondary, each 15 grams, take one month, the symptom of cold syndrome of the stomach, abdominal distention, loose stool, inappetence alleviates gradually.
Test example 2
Have through 50 the Liuweidihuang Oral Liquid that remarkable deficiency of the kidney yin patient takes stomach, trypsin treatment described in embodiment 2, every day takes twice, each 35 can, take one month, be designated as group 1.Simultaneously 50 have remarkable deficiency of the kidney yin patient and take common commercially available LIUWEI DIHUANG WAN, and by specification is taken, and is designated as group 2.Result is as shown in table 1.
Table 1
| Deficiency of the kidney yin symptom is improved (people) | Deficiency and coldness of spleen and stomach symptom (people) | |
| Group 1 | 48 | 2 |
| Group 2 | 49 | 12 |
Interpretation of result
As can be seen from Table 1, group 1 and group 2 all obviously can improve the symptom of deficiency of the kidney yin patient, but existing commercially available LIUWEI DIHUANG WAN can cause part to take the symptom that patient produces cold syndrome of the stomach, abdominal distention, loose stool, inappetence, and taking the Liuweidihuang Oral Liquid of stomach described in embodiment 2, trypsin treatment, the patient that takes producing above-mentioned symptom obviously reduces.
Claims (5)
1. a preparation method for the Liuweidihuang Oral Liquid of stomach, trypsin treatment, is characterized in that, step is as follows:
(1) by the Six-element crude drug in LIUWEI DIHUANG WAN raw material after crushed, obtained material powder, then adds 200 ~ 600 grams of water and refined honey by every hectogram material powder, mix homogeneously, obtained raw slurry;
(2) in the obtained raw slurry of step (1), adding sour adjust pH is 1.5 ~ 2.2, add the pepsin of 20 ~ 60 unit of activity by every hectogram LIUWEI DIHUANG WAN raw material, temperature 36 ~ 38 DEG C, process 2 ~ 3 hours, adjusting PH with base value is 7.8 ~ 8.2, the serosity of obtained pepsin;
(3) in the serosity of the obtained pepsin of step (2), add the trypsin of 20 ~ 50 unit of activity by every hundred milliliters, temperature 36 ~ 38 DEG C, process 2 ~ 3 hours, acid adjust pH to 5.5 ~ 6, obtain the Liuweidihuang Oral Liquid of stomach, trypsin treatment.
2. preparation method as claimed in claim 1, it is characterized in that, the LIUWEI DIHUANG WAN raw material of described step (1) is as follows, is weight portion:
Radix Rehmanniae Preparata 160 parts, Fructus Corni (processed) (wine Fructus Corni) 80 parts, Cortex Moutan 60 parts, Rhizoma Dioscoreae 80 parts, 60 parts, Poria, Rhizoma Alismatis 60 parts, refined honey 7 ~ 10 parts.
3. preparation method as claimed in claim 1, it is characterized in that, the LIUWEI DIHUANG WAN raw material of described step (1) is as follows, is weight portion:
Radix Rehmanniae Preparata 160 parts, Fructus Corni (processed) (wine Fructus Corni) 80 parts, Cortex Moutan 60 parts, Rhizoma Dioscoreae 80 parts, 60 parts, Poria, Rhizoma Alismatis 60 parts, refined honey 16 ~ 22 parts.
4. preparation method as claimed in claim 1, it is characterized in that, the acid in described step (2), (3) is hydrochloric acid.
5. preparation method as claimed in claim 1, it is characterized in that, the alkali of described step (1) is sodium carbonate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310633530.5A CN104666737A (en) | 2013-11-30 | 2013-11-30 | Preparation method of pepsin and trypsin treated Liuwei Dihuang oral liquid |
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| CN201310633530.5A CN104666737A (en) | 2013-11-30 | 2013-11-30 | Preparation method of pepsin and trypsin treated Liuwei Dihuang oral liquid |
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| CN104666737A true CN104666737A (en) | 2015-06-03 |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869587A (en) * | 2010-06-29 | 2010-10-27 | 浙江大学 | Method for extracting anti-gastric cancer active component from water chestnut shells |
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- 2013-11-30 CN CN201310633530.5A patent/CN104666737A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869587A (en) * | 2010-06-29 | 2010-10-27 | 浙江大学 | Method for extracting anti-gastric cancer active component from water chestnut shells |
Non-Patent Citations (1)
| Title |
|---|
| 杨雄志: "《中医药基础》", 30 January 2012 * |
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Application publication date: 20150603 |