CN102266370B - Semi-bionic extraction method for anti-stomach-cancer active substance in Hangzhou white chrysanthemum - Google Patents
Semi-bionic extraction method for anti-stomach-cancer active substance in Hangzhou white chrysanthemum Download PDFInfo
- Publication number
- CN102266370B CN102266370B CN 201110191584 CN201110191584A CN102266370B CN 102266370 B CN102266370 B CN 102266370B CN 201110191584 CN201110191584 CN 201110191584 CN 201110191584 A CN201110191584 A CN 201110191584A CN 102266370 B CN102266370 B CN 102266370B
- Authority
- CN
- China
- Prior art keywords
- buffer
- flos chrysanthemi
- semi
- extract
- filtrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a semi-bionic extraction method for an anti-stomach-cancer active substance in Hangzhou white chrysanthemum. The method comprises the following steps of: (1) smashing dry Hangzhou white chrysanthemum, sieving with a sieve of 40-60 meshes, adding a buffer solution I of which the pH is 2.0-2.2, putting into a water bath at the temperature of 36.5-37.5 DEG C and extracting for 1-2 hours, filtering after extraction, and removing a filtrate 1; and (2) adding a buffer solution II of which the pH is 7.8-8.2 into filter residues obtained in the step (1), putting into a water bath at the temperature of 36.5-37.5 DEG C and extracting for 1-2 hours, filtering after extraction to obtain a filtrate II, and drying the filtrate II to obtain a Hangzhou white chrysanthemum anti-stomach-cancer extract.
Description
Technical field
The present invention relates to the semi-bionic extraction method of anti-gastric cancer active substance in a kind of Flos Chrysanthemi.
Background technology
Flos Chrysanthemi is the dry capitulum of feverfew chrysanthemum (Chrysanthemum morifolium Ramat.), and Tongxiang, main product Zhejiang is one of Zhejiang Province's eight your name's medical materials " Zhejiang eight flavors ".Prove through modern medicine: it has dysentery relieving, antiinflammatory, makes eye bright, blood pressure lowering, blood fat reducing, the effect kept fit, can be used for treating diseases such as jaundice due to damp-heat, stomachache lack of appetite, edema oliguria.At present, the effective ingredient that extracts in the Flos Chrysanthemi comprises chlorogenic acid, volatile oil and flavone compound etc.The antitumor action of Flos Chrysanthemi also is one of its important health-care effect, and report is arranged, and contains beta-elemene in the Flos Chrysanthemi in the volatile oil, and beta-elemene has the broad-spectrum anti-tumor effect.Peng Yufang etc. extract the luteolin that obtains from Flos Chrysanthemi inhibited to breast cancer cell propagation, and normal cell is not had influence.Zhang Tao etc. discover, the Flos Chrysanthemi extract extracorporeal anti-tumor function intensity difference that Different Extraction Method obtains, and water-phase extract is better than the oil phase extract, and water polar extract sample is better than Flos Chrysanthemi Flavonoid substances extract.
According to the Literature Consult result, have only Zhejiang University to open great waves at present and reported that Flos Chrysanthemi extract has the effect that suppresses the stomach cancer cell growth, adopt flooding, ethyl acetate extraction, water extract adopts silicagel column to separate, obtain three samples (main component is not quite clear), above extract all has the growth inhibited effect to stomach cancer cell, and water is better than ethyl acetate extract, silicagel column separates in three samples that obtain, and the sample inhibitory action that polarity is big is better than other two samples.
All the other do not appear in the newspapers.
Semi-bionic extraction is that the biological activity with extract serves as that guiding simulation oral administration and medicine are transported through gastrointestinal tract, be a kind of novel more effective extracting method that the Chinese medicine preparation through the digestive tract administration designs, its more traditional decocting Chinese medicine method is more paid attention to medicine activity in vivo.Chinese scholars has been used for semi-biomimetic method the extraction of effective ingredient such as Ramuli Cinnamomi and Glycyrrhizae Decoction, eucommia bark depressor medicine, has obtained good effect.And the antineoplastic component that adopts semi-bionic extraction to separate in the Flos Chrysanthemi does not appear in the newspapers so far.
Summary of the invention
The technical problem to be solved in the present invention provides the semi-bionic extraction method of anti-gastric cancer active substance in a kind of easy and simple to handle, production cost is low, extraction effect is good Flos Chrysanthemi.
In order to solve the problems of the technologies described above, the invention provides the semi-bionic extraction method of anti-gastric cancer active substance in a kind of Flos Chrysanthemi, may further comprise the steps:
1), the Flos Chrysanthemi of drying pulverized the back cross the 40-60 mesh sieve, adding pH then is the buffer I of 2.0-2.2, places 36.5-37.5 ℃ of water-bath to extract 1-2 hour; The solid-liquid ratio of Flos Chrysanthemi and buffer I is 1g/8-12mL;
After extracting end, filter, discard filtrate I;
2), in step 1) gained filtering residue, add the buffer II that pH is 7.8-8.2, place 36.5-37.5 ℃ of water-bath to extract 1-2 hour, the solid-liquid ratio of the Flos Chrysanthemi in the step 1) and buffer II is 1g/8-12mL;
After extracting end, filter, get filtrate II; The filtrate II drying is handled, and gets the anti-gastric cancer extract of Flos Chrysanthemi.
Improvement as the semi-bionic extraction method of anti-gastric cancer active substance in the Flos Chrysanthemi of the present invention: step 1) and step 2) extraction is all carried out under stirring.
Semi-bionic extraction further improvements in methods as anti-gastric cancer active substance in the Flos Chrysanthemi of the present invention: buffer I is the buffer system of 2.0-2.2 for all pH; Buffer II is the buffer system of 7.8-8.2 for all pH.
Semi-bionic extraction further improvements in methods as anti-gastric cancer active substance in the Flos Chrysanthemi of the present invention: pH is that the buffer I of 2.0-2.2 is glycine-hydrochloride buffer, phthalic acid-hydrochloride buffer, phosphoric acid and phosphate sodium dihydrogen buffer solution or sodium hydrogen phosphate-citrate buffer solution etc.;
PH is that the buffer II of 7.8-8.2 is boric acid-borate buffer solution, sodium hydrogen phosphate-potassium phosphate buffer, potassium dihydrogen phosphate-sodium hydrate buffer solution, sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution or barbital sodium-hydrochloride buffer etc.
Semi-bionic extraction further improvements in methods as anti-gastric cancer active substance in the Flos Chrysanthemi of the present invention: dried step 2) is that lyophilizing is handled.Preferably-50～-60 ℃ lyophilizing is 10～15 hours.
The present invention adopts semi-bionic extraction to extract anti-gastric cancer active substance in the Flos Chrysanthemi, and the imitation oral drugs adopt acid water and the alkaline water of selected pH value at the gastrointestinal transport process, extracts continuously successively and obtains active substance.Semi-bionic extraction and traditional water lifting manipulation compare, and extract is active high, and anti-gastric cancer is effective; Compare with chromatography method, operational approach is simple and feasible, can be extended in the suitability for industrialized production.
Adopt the anti-gastric cancer active substance of method gained of the present invention, using method is as follows: oral, dosage is 5～15mg/kg/ days.
The specific embodiment
The Flos Chrysanthemi of the drying described in following examples refers to: the water content in the Flos Chrysanthemi≤5.0%.Step 1) and step 2) extraction all under stirring, carry out.
The semi-bionic extraction method of anti-gastric cancer active substance in embodiment 1, a kind of Flos Chrysanthemi, carry out following steps successively:
1) pulverizes dry Flos Chrysanthemi 1 gram, cross 60 mesh sieves; The pH that adds 8ml is 2.0 buffer I (that is, solid-liquid ratio is 1g/8mL); Place 37 ℃ of water-baths to extract 2 hours, it is glycine-hydrochloride buffer of 2.0 that this buffer I selects pH for use;
After extracting end, filter, discard filtrate I.
2) pH that adds 10ml in step 1) gained filtering residue is 8.0 buffer II (that is, solid-liquid ratio is 1g/10mL); Place 37 ℃ of water-baths to extract 1 hour, it is boric acid-borate buffer solution of 8.0 that this buffer II selects pH for use;
After extracting end, filter, get filtrate II; Filtrate II prior to-20 ℃ of pre-coolings 30 minutes, in-50 ℃ of dryings 15 hours, is got the anti-gastric cancer extract freeze-drying powder of Flos Chrysanthemi (being the anti-gastric cancer extract of Flos Chrysanthemi) again.
With above-mentioned obtained freeze-drying powder, fixed molten to 20ml with high purity water dissolving, i.e. 0.05g Flos Chrysanthemi/ml.Adopt 0.22 μ m aperture membrane filtration with degerming, gained filtrate is handled stomach cancer cell (SGC7901), and mtt assay is measured cell survival rate, and cell survival rate is 21.0%.
The semi-bionic extraction method of anti-gastric cancer active substance in embodiment 2, a kind of Flos Chrysanthemi, carry out following steps successively:
1) pulverizes dry Flos Chrysanthemi 2 grams, cross 40 mesh sieves; The pH that adds 20ml is 2.2 buffer I (that is, solid-liquid ratio is 1g/10mL); Place 37.5 ℃ of water-baths to extract 1 hour; It is phthalic acid-hydrochloride buffer of 2.2 that this buffer I selects pH for use;
After extracting end, filter, discard filtrate I.
2) pH that adds 24ml in step 1) gained filtering residue is 8.18 buffer II (that is, solid-liquid ratio is 1g/12mL); Place 36.5 ℃ of water-baths to extract 2 hours, it is sodium hydrogen phosphate-potassium phosphate buffer of 8.18 that this buffer II selects pH for use;
After extracting end, filter, get filtrate II; Filtrate II prior to-20 ℃ of pre-coolings 30 minutes, in-55 ℃ of dryings 13 hours, is got the anti-gastric cancer extract freeze-drying powder of Flos Chrysanthemi again.
3) with the obtained freeze-drying powder, fixed molten to 40ml with high purity water dissolving, i.e. 0.05g Flos Chrysanthemi/ml.Adopt 0.22 μ m aperture membrane filtration with degerming, gained filtrate is handled stomach cancer cell (SGC7901), and mtt assay is measured cell survival rate, and cell survival rate is 28.5%.
The semi-bionic extraction method of anti-gastric cancer active substance in embodiment 3, a kind of Flos Chrysanthemi, carry out following steps successively:
1) pulverizes dry Flos Chrysanthemi 5 grams, cross 60 mesh sieves; The pH that adds 60ml is 2.2 buffer I (that is, solid-liquid ratio is 1g/12mL); Place 36.5 ℃ of water-baths to extract 2 hours; It is sodium hydrogen phosphate-citrate buffer solution of 2.2 that this buffer I selects pH for use;
After extracting end, filter, discard filtrate I.
2) pH that adds 40ml in step 1) gained filtering residue is 7.8 buffer II (that is, solid-liquid ratio is 1g/8mL); Place 36.5 ℃ of water-baths to extract 2 hours, it is potassium dihydrogen phosphate-sodium hydrate buffer solution of 7.8 that this buffer II selects for use to pH;
After extracting end, filter, get filtrate II; Prior to-20 ℃ of pre-coolings 30 minutes, ℃ drying was 10 hours again-60 with filtrate II, got the anti-gastric cancer extract freeze-drying powder of Flos Chrysanthemi.
3) with the obtained freeze-drying powder, fixed molten to 100ml with high purity water dissolving, i.e. 0.05g Flos Chrysanthemi/ml.Adopt 0.22 μ m aperture membrane filtration with degerming, gained filtrate is handled stomach cancer cell (SGC7901), and mtt assay is measured cell survival rate, and cell survival rate is 29.5%.
The semi-bionic extraction method of anti-gastric cancer active substance in embodiment 4, a kind of Flos Chrysanthemi, carry out following steps successively:
1) pulverizes dry Flos Chrysanthemi 3 grams, cross 40 mesh sieves; The pH that adds 24ml is 2.0 buffer I (that is, solid-liquid ratio is 1g/8mL); Place 37.5 ℃ of water-baths to extract 1 hour, it is glycine-hydrochloride buffer of 2.0 that this buffer I selects pH for use;
After extracting end, filter, discard filtrate I.
2) pH that adds 30ml in step 1) gained filtering residue is 8.0 buffer II (that is, solid-liquid ratio is 1g/10mL); Place 37 ℃ of water-baths to extract 2 hours, it is sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution of 8.0 that this buffer II selects pH for use;
After extracting end, filter, get filtrate II; Filtrate II prior to-20 ℃ of pre-coolings 30 minutes, in-54 ℃ of dryings 12 hours, is got the anti-gastric cancer extract freeze-drying powder of Flos Chrysanthemi again.
3) with the obtained freeze-drying powder, fixed molten to 60ml with high purity water dissolving, i.e. 0.05g Flos Chrysanthemi/ml.Adopt 0.22 μ m aperture membrane filtration with degerming, gained filtrate is handled stomach cancer cell (SGC7901), and mtt assay is measured cell survival rate, and cell survival rate is 30.1%.
The contrast experiment, in order to prove that the inventor has also carried out following contrast experiment according to the extract obtained superior performance place of the inventive method:
Experiment 1,
1) pulverizes dry Flos Chrysanthemi 5 grams, cross 60 mesh sieves; Add 50ml water (that is, solid-liquid ratio is 1g/10mL); 80 ℃ were extracted 2 hours, filtered, and got filtrate.In-20 ℃ of pre-coolings 30 minutes ,-55 ℃ of dryings 15 hours got Flos Chrysanthemi water extract lyophilized powder.
2) with the obtained freeze-drying powder, fixed molten to 100ml with high purity water dissolving, i.e. 0.05g Flos Chrysanthemi/ml.Adopt 0.22 μ m aperture membrane filtration with degerming, gained filtrate is handled stomach cancer cell (SGC7901), and mtt assay is measured cell survival rate, and cell survival rate is 79.0%.
Experiment 2, will test in 1 step 1) 80 ℃ and extract to be modified in 2 hours in 37 ℃ of water-baths and extracted 2 hours, all the other are with testing 1.Mtt assay is measured cell survival rate, and cell survival rate is about 85.0%.
At last, it is also to be noted that what more than enumerate only is several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (3)
1. the semi-bionic extraction method of anti-gastric cancer active substance in the Flos Chrysanthemi is characterized in that carrying out successively following steps:
1), the Flos Chrysanthemi of drying pulverized the back cross the 40-60 mesh sieve, adding pH then is the buffer I of 2.0-2.2, places 36.5-37.5 ℃ of water-bath to extract 1-2 hour; The solid-liquid ratio of Flos Chrysanthemi and buffer I is 1g/8-12 mL;
After extracting end, filter, discard the filtrate I;
PH is that the buffer I of 2.0-2.2 is glycine-hydrochloride buffer, phthalic acid-hydrochloride buffer or sodium hydrogen phosphate-citrate buffer solution;
2), in step 1) gained filtering residue, add the buffer II that pH is 7.8-8.2, place 36.5-37.5 ℃ of water-bath to extract 1-2 hour, the solid-liquid ratio of the Flos Chrysanthemi in the step 1) and buffer II is 1g/8-12 mL;
After extracting end, filter, get the filtrate II; Filtrate II drying is handled, and gets the anti-gastric cancer extract of Flos Chrysanthemi;
PH is that the buffer II of 7.8-8.2 is boric acid-borate buffer solution, sodium hydrogen phosphate-potassium phosphate buffer, potassium dihydrogen phosphate-sodium hydrate buffer solution, sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution or barbital sodium-hydrochloride buffer.
2. the semi-bionic extraction method of anti-gastric cancer active substance in the Flos Chrysanthemi according to claim 1 is characterized in that: described step 1) and step 2) extraction all under stirring, carry out.
3. the semi-bionic extraction method of anti-gastric cancer active substance in the Flos Chrysanthemi according to claim 1 and 2 is characterized in that: dried described step 2) is that lyophilizing is handled.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110191584 CN102266370B (en) | 2011-07-09 | 2011-07-09 | Semi-bionic extraction method for anti-stomach-cancer active substance in Hangzhou white chrysanthemum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110191584 CN102266370B (en) | 2011-07-09 | 2011-07-09 | Semi-bionic extraction method for anti-stomach-cancer active substance in Hangzhou white chrysanthemum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102266370A CN102266370A (en) | 2011-12-07 |
| CN102266370B true CN102266370B (en) | 2013-08-28 |
Family
ID=45048954
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110191584 Expired - Fee Related CN102266370B (en) | 2011-07-09 | 2011-07-09 | Semi-bionic extraction method for anti-stomach-cancer active substance in Hangzhou white chrysanthemum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102266370B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102786606B (en) * | 2012-09-05 | 2015-05-20 | 龙海市颖欣农业科技有限公司 | Processing method of lentinan |
| CN105820172B (en) * | 2016-03-23 | 2018-04-17 | 安徽农业大学 | A kind of preparation method for having anesthesiophore alkaloid salt hydrochlorate |
| CN111249367A (en) * | 2020-03-27 | 2020-06-09 | 广西欢宝药业有限公司 | A Chinese medicinal preparation for treating osteoporosis, osteonecrosis, and bone generation and promoting blood circulation, and its preparation method |
-
2011
- 2011-07-09 CN CN 201110191584 patent/CN102266370B/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
|---|
| 半仿生提取法_的特点与应用;张兆旺，孙秀梅;《世界科学技术- 中药现代化》;20000131;第2卷(第一期);35－39 * |
| 杭白菊提取物体外抗肿瘤作用研究;张涛;《浙江大学医学院硕士论文》;20090930(第9期);2－3 * |
| 王淑玲.半夏白术天麻汤用半仿生-生物酶提取的系统研究.《山东中医药大学2009届博士学位论文》.2010,(第7期),23-24,67-68. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102266370A (en) | 2011-12-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9770479B2 (en) | Extract of Rehmannia glutinasa Libosch for reducing blood sugar, reducing blood fat, treating leukemia, and preparation method and uses thereof | |
| KR101135824B1 (en) | Composition comprising xanthoceras sorbifolia extracts, compounds isolated from same, methods for preparing same and uses thereof | |
| CN101869587B (en) | Method for extracting anti-gastric cancer active component from water chestnut shells | |
| US8859012B2 (en) | Composition comprising Xanthoceras sorbifolia extracts, compounds isolated from same, methods for preparing same and uses thereof | |
| US7524824B2 (en) | Composition comprising Xanthoceras sorbifolia extracts, compounds isolated from same, methods for preparing same and uses thereof | |
| TWI300352B (en) | Water soluble extract from plant of solanum genus and the preparation process thereof, and pharmaceutical composition containing the water soluble extract | |
| WO2015090180A1 (en) | Sanchi flower arab galactan and preparation method and use thereof | |
| CN106389453B (en) | Flavone glycoside composition | |
| CN101357192B (en) | Separation preparation method of gastrodia elata crude polysaccharide and gastrodia elata alcohol extract using fresh gastrodia elata as raw material | |
| CN102266370B (en) | Semi-bionic extraction method for anti-stomach-cancer active substance in Hangzhou white chrysanthemum | |
| CN113425757A (en) | Dandelion extract and preparation method and application thereof | |
| CN108125946A (en) | Application of the dihydromyricetin in terms of kidney medicine is prepared | |
| JP2000503686A (en) | Pharmaceutical composition for the treatment of hepatitis C, comprising a mixed extract of yellow bamboo skin and Ominaeushi plant | |
| CN114796305B (en) | Preparation method and application of licorice extract | |
| CN105777841A (en) | Method for removing propamocarb in araliaceae saponin extract | |
| CN102614242A (en) | Dropping pill of isatis root and folium isatidis and preparing method thereof | |
| CN102727593A (en) | New use of wild buckwheat rhizome and wild buckwheat rhizome extract in preparation of hypoglycemic medicine and healthcare food | |
| CN112716988A (en) | Application of extract of cissampelos dunculata in preparation of medicine for preventing and/or treating diabetic nephropathy | |
| CN111249271A (en) | Medicament for treating diabetes and preparation method and application thereof | |
| CN107115372B (en) | An antitumor pharmaceutical composition containing folium Apocyni Veneti total flavonoids | |
| CN110279730A (en) | A kind of vepal cwrrant Fischer seed extract and its application in preparation reduction postprandial blood sugar drug | |
| CN1919229B (en) | Bauhinia hupehara C. and its extract for the treatment of diabetes and obesity | |
| CN115671219B (en) | Traditional Chinese medicine composition for treating gout and preparation method and application thereof | |
| CN108354936B (en) | Application of sanguinarine in preparation of medicine for treating animal trichina | |
| CN102351934A (en) | Steroidal alkaloid compounds and pharmaceutical composition thereof, as well as preparation methods and applications of steroidal alkaloid compounds and pharmaceutical composition of compounds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130828 Termination date: 20180709 |