CN113425757A - Dandelion extract and preparation method and application thereof - Google Patents
Dandelion extract and preparation method and application thereof Download PDFInfo
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Abstract
The application provides a dandelion extract and a preparation method and application thereof. The dandelion extract is prepared by the following method: squeezing fresh herba Taraxaci in water, and filtering; wherein the dandelion extract comprises the following components in percentage by weight: 4 to 10 percent of polysaccharide and 0.3 to 1 percent of protein. The dandelion extract is rich in macromolecular substances such as polysaccharide, protein and the like, and the content of micromolecular substances such as phenolic acid, flavone, phenylpropanoids and the like is extremely low, so that the dandelion extract has remarkable antioxidant and anti-inflammatory activities.
Description
Technical Field
The application relates to a dandelion extract and a preparation method and application thereof, belonging to the field of food health products.
Background
Taraxacum mongolicum Hand-Mazz (Latin name) is perennial herb of Taraxacum of Compositae. Root cone, brownish surface, crimple, wavy teeth or deep feathers at the leaf edge, narrowing the base into petioles, reddish purple petioles and main veins, purple red upper scape, dense spider-filiform white long and soft hair: head-shaped inflorescence, general bud bell shape, dark brown lean fruit, white long-crown hair, and flower and fruit period of 4-10 months. Bitter, sweet and cold, entering liver and stomach meridians, has the effects of clearing away heat and toxic materials, relieving swelling, resolving hard mass, inducing diuresis and treating stranguria. Many researches show that the dandelion has the effects of broad-spectrum antibiosis, gastric injury resistance, liver and gallbladder protection, tumor resistance, endotoxin resistance, immunoregulation, oxygen free radical resistance and the like. In addition, the dandelion has influence on female steroid hormone, has lactation promoting effect, and can also be used for breast swelling caused by lactation disorder; has effects in improving cholesterolemia and stimulating estrogen secretion.
At present, the basic research on the drug effect substances of the dandelion mostly focuses on small molecular compounds, such as flavonoids, phenolic acids, coumarins and the like. The most abundant phenolic acid substances in the dandelion are hydroxystyrenic acid derivatives, particularly caffeic acid, chlorogenic acid and the like, which are main pharmacological active ingredients of the dandelion. Chlorogenic acid is a phenylpropanoid compound generated by plants in the aerobic respiration process, and has the functions of antioxidation, antivirus and antibiosis. Especially the biological activity of chlorogenic acid, can promote metabolism of human body, regulate the balance of all functions of human body, etc., and has less toxicity and side effect. Caffeic acid contains two phenolic hydroxyl groups in the structure, is a natural free radical quencher and has excellent antioxidant property, and the existing pharmacological research shows that the caffeic acid has the effects of broad-spectrum antibiosis, inflammation diminishing, cholagogue, diuresis for treating stranguria, blood circulation promoting, stasis removing, cough relieving, phlegm eliminating, antioxidation, anti-tumor and the like, and is used for treating hypertension and cancers. Luteolin is a natural flavonoid compound, and has antiinflammatory, antiallergic, and antitumor pharmacological activities. The aesculetin can increase the content of glutathione in vivo, resist tissue damage and resist the attack of oxygen free radicals in the aging process of organisms, and researches show that the aesculetin has stronger antioxidant activity, and in coumarin compounds, the aesculetin has stronger activities of inhibiting xanthine oxidase and removing oxygen free radicals and has a protective effect on oxidative damage of cell DNA (deoxyribonucleic acid) caused by lipid peroxides.
The prior art mostly focuses on the extraction of such small molecular active ingredients of dandelion, for example, chinese patent CN101982184A, fresh dandelion is used as a starting material, and the method comprises crushing, extracting, concentrating, extracting, separating and purifying, wherein the extraction is to stir and extract the crushed dandelion with acid water with pH of 3-5 at 45-65 ℃ for at least two times, each time is not less than 1.5 hours, filter, separate and combine to obtain an extract; vacuum concentrating the extractive solution into extract, extracting with acetone for at least two times, separating and mixing to obtain extractive solution, and desolventizing the extractive solution to obtain powdered crude extract; purifying the crude extract with chromatography column with fixed phase of Rohm and Hass XAD-8 resin, eluting with deionized water and ethyl acetate in sequence, collecting ethyl acetate eluate, and desolventizing to obtain herba Taraxaci extract with chlorogenic acid and caffeic acid total content of not less than 50%. Chinese patent CN105287682A adopts water extraction, decompression concentration, alcohol precipitation, pH value adjustment, macroporous resin purification, concentration, drying and the like, the dandelion extract obtained by the method has high content of total flavone and caffeic acid and complete components, not only contains effective components of flavonoid and phenolic acid, but also contains components of coumarin, esters and the like, wherein the content of the total flavone is increased to more than 50 percent from 2 percent of medicinal materials, and the content of the caffeic acid is increased to more than 0.8 percent from 0.02 percent. At present, no research on macromolecular active ingredients in taraxacum is available in the field.
Disclosure of Invention
The application aims to provide a dandelion extract and a preparation method and application thereof. The dandelion extract is rich in macromolecular substances such as polysaccharide, protein and the like, and the content of small molecular substances such as chlorogenic acid, caffeic acid, ferulic acid, aesculetin, luteoloside and the like is extremely low, so that the extract has remarkable antioxidant and anti-inflammatory activities.
As one aspect of the present application, there is provided a dandelion extract, which is prepared by the following method: squeezing fresh herba Taraxaci in water, and filtering to obtain herba Taraxaci extract; wherein the dandelion extract comprises the following components in percentage by weight: 4 to 10 percent of polysaccharide and 0.3 to 1 percent of protein.
Preferably, the dandelion extract comprises the following components in percentage by weight: 4.5 to 8 percent of polysaccharide and 0.4 to 0.8 percent of protein. Further preferably, the dandelion extract comprises the following components in percentage by weight: 5 to 7 percent of polysaccharide and 0.45 to 0.65 percent of protein. Most preferably, the dandelion extract comprises the following components in percentage by weight: polysaccharide 5.3%, protein 0.5%.
Preferably, the weight volume ratio of the fresh dandelion to the water is 1: 2-3; the number of squeezing is 1-2.
Preferably, the fresh dandelion is subjected to liquid nitrogen disruption before pressing.
As a second aspect of the present application, there is provided a method for preparing dandelion extract particles, comprising the steps of:
step 1, adding water into fresh dandelion, squeezing and filtering to obtain dandelion extract;
and 2, drying and granulating the dandelion extract prepared in the step 1 to obtain the dandelion extract.
Preferably, the weight volume ratio of the fresh dandelion and the water in the step 1 is 1: 2-3; the number of squeezing is 1-2.
Preferably, the drying in step 2 is spray drying or freeze drying; wherein the temperature of spray drying is 110-120 ℃; further preferably 115 ℃. The freeze drying temperature is-50 to-60 ℃.
Preferably, the fresh dandelion is subjected to liquid nitrogen disruption before pressing.
As a third aspect of the present application, the present application provides a use of a dandelion extract for preparing a medicament with antioxidant activity, wherein the dandelion extract comprises the following components by weight percentage: 4 to 10 percent of polysaccharide and 0.3 to 1 percent of protein.
Preferably, the dandelion extract comprises the following components in percentage by weight: 4.5 to 8 percent of polysaccharide and 0.4 to 0.8 percent of protein.
Further preferably, the dandelion extract comprises the following components in percentage by weight: 5 to 7 percent of polysaccharide and 0.45 to 0.65 percent of protein.
Most preferably, the dandelion extract comprises the following components in percentage by weight: polysaccharide 5.3%, protein 0.5%.
Preferably, the dandelion extract is prepared by the following method: squeezing fresh herba Taraxaci in water, and filtering to obtain herba Taraxaci extract.
Further preferably, the weight volume ratio of the fresh dandelion to the water is 1: 2-3; the number of squeezing is 1-2.
Further preferably, the fresh dandelion is subjected to liquid nitrogen disruption before pressing.
As a fourth aspect of the present application, the present application provides a use of the dandelion extract for preparing a medicament with anti-inflammatory activity, wherein the dandelion extract comprises the following components by weight percentage: 4 to 10 percent of polysaccharide and 0.3 to 1 percent of protein.
Preferably, the dandelion extract comprises the following components in percentage by weight: 4.5 to 8 percent of polysaccharide and 0.4 to 0.8 percent of protein.
Further preferably, the dandelion extract comprises the following components in percentage by weight: 5 to 7 percent of polysaccharide and 0.45 to 0.65 percent of protein.
Most preferably, the dandelion extract comprises the following components in percentage by weight: polysaccharide 5.3%, protein 0.5%.
Preferably, the dandelion extract is prepared by the following method: squeezing fresh herba Taraxaci in water, and filtering to obtain herba Taraxaci extract.
Further preferably, the weight volume ratio of the fresh dandelion to the water is 1: 2-3; the number of squeezing is 1-2.
Further preferably, the fresh dandelion is subjected to liquid nitrogen disruption before pressing.
The fresh dandelion or the fresh dandelion is the dandelion which is just harvested and is not dried and processed; the dry medicine or the dry dandelion is dried dandelion.
The beneficial effect of this application: in the prior art, active ingredients of dandelion are researched and mainly emphasized on small molecular compounds, such as flavonoids, phenolic acids and coumarins. The application unexpectedly finds that the macromolecular compound in the dandelion is a main component for playing anti-inflammatory and anti-oxidation roles. The prepared dandelion extract is rich in macromolecular components such as polysaccharide, protein and the like by using fresh dandelion as a raw material and adopting a squeezing process, and the content of active small molecular components (such as chlorogenic acid, caffeic acid, ferulic acid, aesculetin and luteolin) disclosed in the prior art is extremely low. Pharmacological experiment results show that the effect of the dandelion extract rich in macromolecular components, which is prepared by taking fresh dandelion as a raw material, in the aspects of inflammation resistance and oxidation resistance is obviously superior to that of a fresh dandelion water extract, a fresh dandelion alcohol extract and a dry dandelion water extract.
The preparation method is simple, and the production cost is greatly saved. In the prior art, a water extraction or alcohol extraction process is mostly adopted, and although the cost is low, the recovery of the solvent is time-consuming and labor-consuming; the cost of extraction is high by taking ethanol as a solvent. In addition, in the prior art, in order to obtain an extract with high content of phenolic acid or flavonoid components, the crude extract is generally further separated and purified, and the processes of acid water extraction, organic solvent extraction, alcohol precipitation, chromatographic column separation and the like are adopted, so that the process is complex and the cost is high. And this application only needs to carry out liquid nitrogen to bright dandelion and smashes, and simple process has practiced thrift the cost greatly.
Detailed Description
The following examples are given for the detailed implementation and specific operation of the present invention, but the scope of the present invention is not limited to the following examples.
Example 1 comparison of the efficacy and chemical content of extracts prepared by different extraction methods for Taraxacum Mongolicum
1. Material
1.1 Experimental drugs
Homogenizing fresh medicine: taking 20g of fresh dandelion, adding a small amount of water, fully grinding for multiple times, combining grinding liquids, and fixing the volume to 40 mL;
fresh medicinal water extract: reflux-extracting 20g of fresh herba Taraxaci with 6 times of water for 30min for 2 times, mixing water decoctions, and concentrating to 40 mL;
dry drug water extract: extracting 20g of dried herba Taraxaci with 6 times of water under reflux for 30min for 2 times, mixing decoctions, and concentrating to 40 mL;
positive control drug: aspirin enteric-coated tablets, purchased from Shenwei pharmaceutical Co., Ltd, under batch No. 1004172, were ground and prepared into a 15mg/mL solution with distilled water.
1.2 Experimental animals
40 healthy Kunming mice, SPF grade, half male and female, weight 18-20g, purchased from Beijing Wintonlihua laboratory animal technology Co., Ltd, license number: SYXK- (Jing) 2014-. The animal feed is bred in the center of the research institute of basic theory of traditional Chinese medicine of Chinese academy of traditional Chinese medicine, the cleaning level, the qualification number: SYXK (Kyoto) 2010-0032. The temperature of the animal room is 20-26 ℃, the humidity is 40-60%, and the illumination is changed for 12 hours day and night. The clean-grade rat maintenance feed is purchased from Beijing Ke Australian cooperative feed Co., Ltd, and the feed production enterprise reviews the certification number: kyoto trial (2009) 06166. Production batch number: 14063212.
1.3 instruments
Electronic balance (mertler-toledo instruments (china) ltd, switzerland).
2. Method of producing a composite material
2.1 anti-inflammatory assay
The difference of the anti-inflammatory activity of the experimental drug was evaluated using a xylene-induced mouse ear swelling model in this experiment.
50 KM mice were randomly divided into 5 groups of 10 mice each. Respectively including a fresh medicine homogenate group, a fresh medicine water extraction group, a dry medicine water extraction group, a positive medicine (aspirin) and a model group. The solutions of each group were administered by gavage with 0.2ml/10g body weight for 3 days 1 time per day, and the model group was administered with physiological saline. 1h after the third day of administration, mice were coated with 50. mu.L of xylene-based ear swelling model in the right ear. And (3) killing the mouse by a cervical dislocation method 1h after the model is made, cutting two ears along an auricle base line, punching round ear pieces on the same parts of the left ear and the right ear of the mouse by using a puncher with the diameter of 8mm, and weighing. In the experiment of mouse ear swelling caused by dimethylbenzene, the swelling degree is equal to the mass of a right ear tablet-the mass of a left ear tablet, and the swelling inhibition rate (%) of the medicament is equal to (the swelling degree of a physiological saline group-the swelling degree of an administration group)/the swelling degree of the physiological saline group multiplied by 100%.
All data are expressed in x +/-s, two groups of data are compared by using t test, a plurality of groups of data are compared by using one-factor variance analysis, and P is less than 0.05 as a difference significance test standard.
The results are shown in Table 1.
TABLE 1 comparison of anti-inflammatory Activity of different Dandelion extracts
Grouping | Mouse ear swelling Rate (average) |
Fresh medicine homogenate group | 0.47±0.16* |
Fresh medicinal water extract group | 0.71±0.37* |
Dry medicinal water extract group | 0.65±0.29 |
Positive control group | 0.52±0.22* |
Model set | 1.12±0.32 |
Indicates that the difference is significant compared with the model group, wherein P is less than 0.05
And (4) conclusion: different extracts of dandelion have anti-inflammatory effect, but the fresh medicine homogenate group is obviously superior to other extract groups.
2.2 Oxidation resistance test
Collecting 500 μ L of different herba Taraxaci extracts (fresh medicine homogenate, fresh medicine water extract, and dry medicine water extract), freeze drying, adding 500 μ L of anhydrous ethanol for dissolving, and centrifuging to obtain supernatant. All test sample solutions were serially diluted to 1.00, 0.50, 0.25, 0.125, 0.0625, 0.03125g/mL solutions. Vitamin C standard is used as a positive control drug.
100 mu L of each sample solution with different concentrations is placed in each hole of a 96-hole plate, 100 mu L of absolute ethyl alcohol is placed in a blank, then 100 mu L of DPPH preparation solution is completely added, the mixture is placed in a dark incubator at 37 ℃ for reaction for 0.5h, and then the absorbance A is measured at 520nm of an enzyme-labeling instrument. And (4) calculating the clearance rate of the sample on DPPH free radicals by taking Vc as a positive control.
DPPH clearance (%) - (a)0-A)/A0*100%(A0Absorbance for blank, and a for sample). IC of DPPH radical Activity of samples was calculated using GraphPad Prism 5 software50. The results are shown in Table 2.
TABLE 2 results of different dandelion extracts in anti-oxidation experiments
Grouping | IC50 |
Fresh medicine homogenate group | 3.02 |
Fresh medicinal water extract group | 15.08 |
Dry medicinal water extract group | 22.69 |
Positive control group | 0.28 |
And (4) conclusion: IC (integrated circuit)50The more valueThe stronger the small antioxidant capacity is, the more excellent the antioxidant effect of the fresh medicine homogenate group is than that of other extracts.
2.3 comparison of component content
2.3.1 comparison of Total protein content
Taking the fresh medicine homogenate, the fresh medicine water extract and the dry medicine water extract, and preparing into 1 percent solution by using normal saline. The total protein content of the samples was determined using the coomassie brilliant blue kit. The results are shown in Table 3.
TABLE 3 comparison of the Total protein content of different Dandelion extracts
The results show that: the total protein content in the homogenate of the fresh medicine is obviously higher than that of the aqueous extract of the fresh medicine and the aqueous extract of the dry medicine.
2.3.2 comparison of Total polysaccharide content
Preparation of control solutions: a control sample of anhydrous glucose was precisely weighed and placed in a 25ml volumetric flask, and dissolved in distilled water to prepare a control solution containing 1.962mg of glucose per ml.
Preparation of a test solution:
precisely sucking 1ml of each of the fresh medicine homogenate, the fresh medicine water extract, the dry medicine water extract and the distilled water, respectively placing the fresh medicine homogenate, the fresh medicine water extract, the dry medicine water extract and the distilled water into 10ml test tubes with plugs, respectively and precisely adding 1.0ml of 4% phenol solution and 7.0ml of concentrated sulfuric acid, shaking up, then preserving the temperature in a water bath at 40 ℃ for 30min, taking out, placing in an ice water bath, and cooling to room temperature. The absorbance at the wavelength of 485nm was measured using water as a blank solvent, and each group was repeated once. The results are shown in Table 4.
TABLE 4 comparison of polysaccharide content in different dandelion extracts
The results show that: the polysaccharide content in the fresh medicine homogenate is 5 times and 2 times of the polysaccharide content in the fresh medicine water extract and the dry medicine water extract respectively.
2.3.3 comparison of Small molecule component content
Preparation of test solution
Respectively taking the fresh medicine homogenate, the fresh medicine water extract and the dry medicine water extract, diluting with 50% methanol until the crude drug amount is 0.1g crude drug/ml, and filtering with 0.45 μm filter membrane to obtain a test solution;
HPLC conditions: agilent SB C18 column (2.1X 50mm, 1.8 μm). Mobile phase composition: aqueous phase (a) was 0.1% formic acid water; the organic phase (B) is acetonitrile; the flow rate was 0.3 mL/min. Gradient: 0-1min, 10% B; 1.1-7min, 30% B, the running time is 7min, and the later running time is 6 min; the sample injection amount is 2 uL; the column temperature was 35 ℃.
Mass spectrum conditions: agilent 6410 mass spectrum, ESI source. Detecting the negative ions in an MRM mode; capillary voltage: 4000V; gas flow rate: 12L/min; atomizer pressure: 40 psi.
The standard curve of the component to be measured is shown in Table 5; the results are shown in Table 6.
TABLE 5 Standard Curve of the ingredients to be tested
TABLE 6 results of content measurement (μ g/g)
Composition (I) | Fresh medicine homogenate | Fresh medicinal water extract | Dry medicinal aqueous extract |
All-grass of greeneryAcid(s) | 1.20 | 11.97 | 5.06 |
Ferulic acid | 0.08 | 0.54 | 0.83 |
Fraxinin B | 0.01 | 0.27 | 0.21 |
Luteolin glycoside | * | 1.73 | 1.94 |
Caffeic acid | 0.17 | 11.13 | 8.34 |
Note: indicates no detection.
The results show that the contents of phenolic acid components, flavonoid components and phenylpropanoid components in the fresh medicine homogenate are obviously lower than those of the fresh medicine water extract and the dry medicine water extract.
The results show that the anti-inflammatory and antioxidant effects of the homogenate of the fresh medicine are superior to those of the aqueous extract of the fresh medicine and the aqueous extract of the dry medicine; the total protein and total polysaccharide content in the fresh medicine homogenate is higher than that of the fresh medicine water extract and the dry medicine water extract; the contents of phenolic acids, flavonoids and phenylpropanoids in the fresh medicine homogenate are far lower than those of the fresh medicine water extract and the dry medicine water extract; the application shows that the extract prepared by taking the fresh dandelion herb as the raw material medicine by adopting the squeezing method can effectively retain macromolecular active ingredients such as polysaccharide, protein and the like, and exert the anti-inflammatory and antioxidant activities.
Example 2
Taking 20g of fresh dandelion, adding a proper amount of water, juicing by a juicer, and filtering to obtain the fresh dandelion homogenate.
The contents of protein and polysaccharide determined by the method of example 1 are respectively: protein: 0.62 percent and 5.8 percent of polysaccharide.
Example 3
Taking 20g of fresh dandelion, crushing by liquid nitrogen, adding a proper amount of water, juicing by a juicer, and filtering to obtain the fresh dandelion homogenate.
The contents of protein and polysaccharide determined by the method of example 1 are respectively: protein: 0.75 percent and polysaccharide 6.4 percent.
Example 4
Respectively homogenizing the fresh dandelion herbs prepared in the embodiments 1-3, carrying out spray drying in a spray dryer at the spray drying temperature of 115 ℃, collecting the dried products, and sieving with a 100-mesh sieve to obtain dandelion spray dried powder; mixing the spray-dried powder with sodium carboxymethyl starch, sieving with 60-80 mesh sieve, adding appropriate amount of anhydrous ethanol, and dry granulating to obtain herba Taraxaci granule.
Example 5
Respectively homogenizing the fresh dandelion herbs prepared in the embodiments 1-3, freeze-drying in a freeze dryer at-55 ℃, collecting the dried product, and sieving with a 100-mesh sieve to obtain the freeze-dried dandelion powder; mixing the lyophilized powder with sodium carboxymethyl starch, sieving with 60-80 mesh sieve, adding appropriate amount of anhydrous ethanol, and dry granulating to obtain herba Taraxaci granule.
Claims (10)
1. A dandelion extract, which is characterized by being prepared by the following method: squeezing fresh herba Taraxaci in water, and filtering; wherein the dandelion extract comprises the following components in percentage by weight: 4 to 10 percent of polysaccharide and 0.3 to 1 percent of protein.
2. The dandelion extract according to claim 1, wherein the dandelion extract comprises the following components in percentage by weight: 4.5 to 8 percent of polysaccharide and 0.4 to 0.8 percent of protein; or, polysaccharide 5-7%, protein 0.45-0.65%; or 5.3% of polysaccharide and 0.5% of protein.
3. A preparation method of dandelion extract particles is characterized by comprising the following steps:
step 1, adding water into fresh dandelion, squeezing and filtering to obtain dandelion extract;
and 2, drying and granulating the dandelion extract prepared in the step 1 to obtain the dandelion extract.
4. The preparation method according to claim 3, wherein the weight volume ratio of the fresh dandelion and the water in the step 1 is 1: 2-3; the number of squeezing is 1-2.
5. The method according to claim 3, wherein the drying in step 2 is spray drying or freeze drying; wherein the temperature of spray drying is 110-120 ℃; the freeze drying temperature is-50 to-60 ℃.
6. The method of claim 3, wherein the fresh dandelion herb of step 1 is subjected to liquid nitrogen disruption prior to expression.
7. The application of the dandelion extract in preparing the medicine with the antioxidant activity is characterized in that the dandelion extract is prepared by the following method: squeezing fresh herba Taraxaci in water, and filtering; wherein the dandelion extract comprises the following components in percentage by weight: 4 to 10 percent of polysaccharide and 0.3 to 1 percent of protein.
8. The use of claim 7, wherein the dandelion extract comprises the following ingredients in weight percent: 4.5 to 8 percent of polysaccharide and 0.4 to 0.8 percent of protein; or, polysaccharide 5-7%, protein 0.45-0.65%; or 5.3% of polysaccharide and 0.5% of protein.
9. The application of the dandelion extract in preparing the medicine with anti-inflammatory activity is characterized in that the dandelion extract is prepared by the following method: squeezing fresh herba Taraxaci in water, and filtering; wherein the dandelion extract comprises the following components in percentage by weight: 4 to 10 percent of polysaccharide and 0.3 to 1 percent of protein.
10. The use of claim 9, wherein the dandelion extract comprises the following ingredients in weight percent: 4.5 to 8 percent of polysaccharide and 0.4 to 0.8 percent of protein; or, polysaccharide 5-7%, protein 0.45-0.65%; or 5.3% of polysaccharide and 0.5% of protein.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113925888A (en) * | 2021-11-10 | 2022-01-14 | 广西农业职业技术大学 | Preparation method and application of dandelion powder |
CN115177649A (en) * | 2022-07-19 | 2022-10-14 | 中国农业科学院农产品加工研究所 | Dandelion extract and preparation method and application thereof |
CN115634262A (en) * | 2021-10-19 | 2023-01-24 | 李景龙 | Anti-inflammatory composition and application and preparation method thereof |
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CN115634262A (en) * | 2021-10-19 | 2023-01-24 | 李景龙 | Anti-inflammatory composition and application and preparation method thereof |
CN115634262B (en) * | 2021-10-19 | 2024-01-30 | 李景龙 | Anti-inflammatory composition and application and preparation method thereof |
CN113925888A (en) * | 2021-11-10 | 2022-01-14 | 广西农业职业技术大学 | Preparation method and application of dandelion powder |
CN115177649A (en) * | 2022-07-19 | 2022-10-14 | 中国农业科学院农产品加工研究所 | Dandelion extract and preparation method and application thereof |
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