CN106832023A - A kind of method that polysaccharide is extracted from dandelion - Google Patents
A kind of method that polysaccharide is extracted from dandelion Download PDFInfo
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- CN106832023A CN106832023A CN201510894571.9A CN201510894571A CN106832023A CN 106832023 A CN106832023 A CN 106832023A CN 201510894571 A CN201510894571 A CN 201510894571A CN 106832023 A CN106832023 A CN 106832023A
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention discloses a kind of method that polysaccharide is extracted from dandelion, its operating procedure is as follows：1. pretreatment, fresh radix tarxaci is cleaned, and is cut into pieces, and 60 DEG C of dryings are clayed into power to constant weight with pulverizer, crosses 40-60 mesh sieves standby；2. degreasing, dandelion powder is wrapped with filter paper, is put into apparatus,Soxhlet's, 40 DEG C of petroleum ether backflow degreasing 2-3h；3. the removal of monose and oligosaccharides, the filter paper bag after degreasing is air-dried, and uses 70-90% alcohol refluxs, to remove the monose in sample, oligosaccharides and other small-molecule substances, the total sugar content of sample after backflow is determined with anthrone-sulphate method；4. hot water extraction, 60-80 DEG C, material-water ratio is 1：20-1:40, extraction time 2-4h, extract 1-3 times.This method preparation process is simple, steady quality, the rate of recovery is high, is adapted to industrialized production, with certain application prospect and market value.
Description
Technical field
The invention belongs to field of biological pharmacy, especially a kind of method that polysaccharide is extracted from dandelion.
Background technology
Dandelion is common farmland weed, is also important Chinese medicine, belongs to one of important protection kind of country, is had
Effect of heat-clearing, dissipating bind and inducing diuresis for treating strangurtia, is clinically mainly used in treating the diseases such as newborn pyogenic infections from tumour or sore, dandelion rich in sterol,
Polysaccharide, many experiments show that polysaccharide is important informational molecule, participate in many physiology and pathologic process, some crude drugs
Thing its active ingredient polysaccharide has this various pharmacological activity, medically have antitumor, antiviral, anti-aging, radioresistance,
Many effects such as anti-mutation, hypoglycemic, enhancing be immune.
Many method extraction times that polysaccharide is extracted from dandelion are long, and extraction efficiency is low, and have certain pollution to environment.
The content of the invention
It is an object of the invention to be directed to the problem that the current method that polysaccharide is extracted from dandelion is present, there is provided a kind of from Pu
The method of public English-Chinese extraction polysaccharide.The method polysaccharide extract rate and purity are obtained for very big raising, and the method has and carries
Take that the time is short, method is simple, recovery rate is high, steady quality the advantages of, be adapted to industrialized production.To achieve the above object,
The technical solution adopted by the present invention is：
A kind of method that polysaccharide is extracted from dandelion, comprises the following steps：
(1) pretreatment, fresh radix tarxaci is cleaned, and is cut into pieces, and 60 DEG C of dryings use pulverizer to constant weight
Clay into power, cross 40-60 mesh sieves standby；
(2) degreasing, dandelion powder is wrapped with filter paper, is put into apparatus,Soxhlet's, 40 DEG C of petroleum ether backflow degreasing 2-3h；
(3) removal of monose and oligosaccharides, the filter paper bag after degreasing is air-dried, and 70-90% alcohol refluxs is used, to remove sample
Monose, oligosaccharides and other small-molecule substances in product, the total sugar content of sample after backflow is determined with anthrone-sulphate method；
(4) hot water extraction, 60-80 DEG C, material-water ratio is 1：20-1:40, extraction time 2-4h, extract 1-3 times；
(5) concentrate, extract solution is concentrated with Rotary Evaporators；
(6) albumen, Sevag methods, to 1/4 times of chloroform-n-butanol of volume of addition, 3000-4000r/min in extract solution are taken off
Centrifugation 5-10min, middle level white flock precipitate is protein, takes supernatant, repeats 5-6 times, to centre without obvious sediment
Untill；
(7) alcohol analysis, after taking off albumen, to 80-95% ethanol is added in extract solution, 3000-4000r/min is centrifuged 5-10min,
Precipitation is Thick many candies；
(8) purify, Thick many candies are redissolved in water, dialyse 24-36h, with 80-95% ethanol precipitation polysaccharide, 3000-4000r/min
Centrifugation 5-10min, polysaccharide precipitation is washed 3-5 times with absolute ethyl alcohol, acetone respectively, is vacuum dried to obtain refined polysaccharide.
As further scheme, in step (1) in pretreatment, dandelion is crushed to 40 mesh.
Used as further scheme, the degreasing return time described in step (2) is 2.5h.
Used as further scheme, ethanol described in step (3) is 80% ethanol.
Used as further scheme, hot water extraction's temperature is 80 DEG C in step (4), and material-water ratio is 1:30, extraction time is
3h, extraction time is 2 times.
Used as further scheme, centrifugal speed is 3500r/min in step (6), and 5min is centrifuged, and is repeated 5 times.
Used as further scheme, step (7) concentration of alcohol is 90%, and centrifugal speed is 3500r/min, and 5min is centrifuged.
Used as further scheme, step (8) dialysis time is 36h, and concentration of alcohol is 85%, and centrifugal speed is 3500r/min,
Centrifugation 5min, polysaccharide precipitation is washed 3 times with absolute ethyl alcohol, acetone respectively, is vacuum dried to obtain refined polysaccharide.
The recovery rate and purity of the extracting method in the present invention are obtained for very big raising, with extraction time is short, method
Simply, recovery rate is high, steady quality, the rate of recovery high the advantages of.
The method have the advantages that：
1. the rate of recovery of polysaccharide reaches more than 97% in the present invention.
2. extracting method of the invention has that extraction time is short, method is simple, extraction efficiency is high, steady quality, the rate of recovery
High the advantages of, it is adapted to industrialized production.
Specific embodiment
With reference to specific embodiment, the invention will be further described
Embodiment 1
A kind of method that polysaccharide is extracted from dandelion, comprises the following steps：
(1) dandelion sample 100g is weighed, is dried, be crushed to certain particle size, 40 mesh sieves are standby；
(2) degreasing, dandelion powder is wrapped with filter paper, is put into apparatus,Soxhlet's, 40 DEG C of petroleum ether backflow degreasing 2.5h；
(3) removal of monose and oligosaccharides, the filter paper bag after degreasing is air-dried, and uses 80% alcohol reflux, with removing sample
Monose, oligosaccharides and other small-molecule substances, the total sugar content of sample after backflow is determined with anthrone-sulphate method；
(4) hot water extraction, 80 DEG C, material-water ratio is 1：30, extraction time 3h, extract 2 times；
(5) concentrate, extract solution is concentrated with Rotary Evaporators；
(6) albumen is taken off, Sevag methods are centrifuged to 1/4 times of chloroform-n-butanol of volume, 3500r/min is added in extract solution
5min, middle level white flock precipitate is protein, takes supernatant, is repeated 5 times, to centre without obvious sediment；
(7) alcohol analysis, after taking off albumen, to 90% ethanol is added in extract solution, 3500r/min centrifugation 5min, precipitation is
Thick many candies；
(8) purify, Thick many candies are redissolved in water, dialyse 36h, with 85% ethanol precipitation polysaccharide, 3500r/min centrifugations
5min, polysaccharide precipitation is washed 3 times with absolute ethyl alcohol, acetone respectively, is vacuum dried to obtain refined polysaccharide.The method obtains radix tarxaci
Portion's polysaccharide accounts for the 60.08% of radix tarxaci dry weight, and the rate of recovery of polysaccharide reaches 97%.
Embodiment 2
A kind of method that polysaccharide is extracted from dandelion, comprises the following steps：
(1) dandelion sample 100g is weighed, is dried, be crushed to certain particle size, 40 mesh sieves are standby；
(2) degreasing, dandelion powder is wrapped with filter paper, is put into apparatus,Soxhlet's, 40 DEG C of petroleum ether backflow degreasing 2h；
(3) removal of monose and oligosaccharides, the filter paper bag after degreasing is air-dried, and uses 70% alcohol reflux, with removing sample
Monose, oligosaccharides and other small-molecule substances, the total sugar content of sample after backflow is determined with anthrone-sulphate method；
(4) hot water extraction, 60 DEG C, material-water ratio is 1：20, extraction time 2h, extract 2 times；
(5) concentrate, extract solution is concentrated with Rotary Evaporators；
(6) albumen is taken off, Sevag methods are centrifuged to 1/4 times of chloroform-n-butanol of volume, 3000r/min is added in extract solution
10min, middle level white flock precipitate is protein, takes supernatant, is repeated 5 times, to centre without obvious sediment；
(7) alcohol analysis, after taking off albumen, to 80% ethanol is added in extract solution, 3000r/min is centrifuged 10min, and precipitation is
It is Thick many candies；
(8) purify, Thick many candies are redissolved in water, dialyse 24h, with 80% ethanol precipitation polysaccharide, 3000r/min centrifugations
10min, polysaccharide precipitation is washed 3 times with absolute ethyl alcohol, acetone respectively, is vacuum dried to obtain refined polysaccharide.The method obtains radix tarxaci
Portion's polysaccharide accounts for the 56.12% of radix tarxaci dry weight, and the rate of recovery of polysaccharide reaches 93%.
Embodiment 3
A kind of method that polysaccharide is extracted from dandelion, comprises the following steps：
(1) dandelion sample 100g is weighed, is dried, be crushed to certain particle size, 40 mesh sieves are standby；
(2) degreasing, dandelion powder is wrapped with filter paper, is put into apparatus,Soxhlet's, 40 DEG C of petroleum ether backflow degreasing 3h；
(3) removal of monose and oligosaccharides, the filter paper bag after degreasing is air-dried, and uses 90% alcohol reflux, with removing sample
Monose, oligosaccharides and other small-molecule substances, the total sugar content of sample after backflow is determined with anthrone-sulphate method；
(4) hot water extraction, 70 DEG C, material-water ratio is 1:40, extraction time 4h, extract 2 times；
(5) concentrate, extract solution is concentrated with Rotary Evaporators；
(6) albumen is taken off, Sevag methods are centrifuged to 1/4 times of chloroform-n-butanol of volume, 4000r/min is added in extract solution
5min, middle level white flock precipitate is protein, takes supernatant, is repeated 5 times, to centre without obvious sediment；
(7) alcohol analysis, after taking off albumen, to 95% ethanol is added in extract solution, 4000r/min centrifugation 5min, precipitation is
Thick many candies；
(8) purify, Thick many candies are redissolved in water, dialyse 36h, with 95% ethanol precipitation polysaccharide, 4000r/min centrifugations
5min, polysaccharide precipitation is washed 3 times with absolute ethyl alcohol, acetone respectively, is vacuum dried to obtain refined polysaccharide.The method obtains radix tarxaci
Portion's polysaccharide accounts for the 58.26% of radix tarxaci dry weight, and the rate of recovery of polysaccharide reaches 90%.
The above, is only presently preferred embodiments of the present invention, not makees any formal to technical scheme
Limitation.Every any simple modification made to above example according to technical spirit of the invention, equivalent variations and repair
Decorations, still fall within the range of technical scheme.
Claims (8)
1. it is a kind of from dandelion extract polysaccharide method, it is characterised in that its operating procedure is as follows：
(1) pretreatment, fresh radix tarxaci is cleaned, and is cut into pieces, and 60 DEG C of dryings use pulverizer to constant weight
Clay into power, cross 40-60 mesh sieves standby；
(2) degreasing, dandelion powder is wrapped with filter paper, is put into apparatus,Soxhlet's, 40 DEG C of petroleum ether backflow degreasing 2-3h；
(3) removal of monose and oligosaccharides, the filter paper bag after degreasing is air-dried, and 70-90% alcohol refluxs is used, to remove sample
Monose, oligosaccharides and other small-molecule substances in product, the total sugar content of sample after backflow is determined with anthrone-sulphate method；
(4) hot water extraction, 60-80 DEG C, material-water ratio is 1：20-1:40, extraction time 2-4h, extract 1-3 times；
(5) concentrate, extract solution is concentrated with Rotary Evaporators；
(6) albumen, Sevag methods, to 1/4 times of chloroform-n-butanol of volume of addition, 3000-4000r/min in extract solution are taken off
Centrifugation 5-10min, middle level white flock precipitate is protein, takes supernatant, repeats 5-6 times, to centre without obvious sediment
Untill；
(7) alcohol analysis, after taking off albumen, to 80-95% ethanol is added in extract solution, 3000-4000r/min is centrifuged 5-10min,
Precipitation is Thick many candies；
(8) purify, Thick many candies are redissolved in water, dialyse 24-36h, with 80-95% ethanol precipitation polysaccharide, 3000-4000r/min
Centrifugation 5-10min, polysaccharide precipitation is washed 3-5 times with absolute ethyl alcohol, acetone respectively, is vacuum dried to obtain refined polysaccharide.
2. it is according to claim 1 it is a kind of from dandelion extract polysaccharide method, it is characterised in that step (1)
In middle pretreatment, dandelion is crushed to 40 mesh.
3. it is according to claim 1 it is a kind of from dandelion extract polysaccharide method, it is characterised in that step (2)
Described in degreasing return time be 2.5h.
4. it is according to claim 1 it is a kind of from dandelion extract polysaccharide method, it is characterised in that step (3)
Described in ethanol be 80% ethanol.
5. it is according to claim 1 it is a kind of from dandelion extract polysaccharide method, it is characterised in that step (4)
In, hot water extraction's temperature is 80 DEG C, and material-water ratio is 1:30, extraction time is 3h, and extraction time is 2 times.
6. it is according to claim 1 it is a kind of from dandelion extract polysaccharide method, it is characterised in that step (6)
In, centrifugal speed is 3500r/min, and 5min is centrifuged, and is repeated 5 times.
7. it is according to claim 1 it is a kind of from dandelion extract polysaccharide method, it is characterised in that step (7)
In, concentration of alcohol is 90%, and centrifugal speed is 3500r/min, and 5min is centrifuged.
8. it is according to claim 1 it is a kind of from dandelion extract polysaccharide method, it is characterised in that step (8)
In, dialysis time is 36h, and concentration of alcohol is 85%, and centrifugal speed is 3500r/min, and 5min, polysaccharide precipitation point is centrifuged
Do not washed 3 times with absolute ethyl alcohol, acetone, be vacuum dried to obtain refined polysaccharide.
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| CN201510894571.9A CN106832023A (en) | 2015-12-07 | 2015-12-07 | A kind of method that polysaccharide is extracted from dandelion |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113425757A (en) * | 2021-06-08 | 2021-09-24 | 中国中医科学院中医基础理论研究所 | Dandelion extract and preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113425757A (en) * | 2021-06-08 | 2021-09-24 | 中国中医科学院中医基础理论研究所 | Dandelion extract and preparation method and application thereof |
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Application publication date: 20170613 |