CN101993902A - Method for extracting flavonoids compounds from asparagus and leftovers thereof - Google Patents
Method for extracting flavonoids compounds from asparagus and leftovers thereof Download PDFInfo
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- CN101993902A CN101993902A CN 201010522492 CN201010522492A CN101993902A CN 101993902 A CN101993902 A CN 101993902A CN 201010522492 CN201010522492 CN 201010522492 CN 201010522492 A CN201010522492 A CN 201010522492A CN 101993902 A CN101993902 A CN 101993902A
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Abstract
The invention belongs to the technical field of extraction of flavonoids compounds, and particularly relates to a method for extracting flavonoids compounds from asparagus and leftovers thereof. Compared with the prior art, the extraction method adopts a biological enzymolysis technique and an ultrasonic wave principle and comprises the following steps of: cleaning the asparagus and leftovers thereof, drying, taking out and then crushing, adding water according to the feed liquid ratio of 1:10 for mixing, then adding cellulase with 5% by mass of the asparagus powder, performing enzymolysis for 100 minutes at the ambient temperature of 50 DEG C with the pH value of 5.5, then adding ethanol, extracting the feed liquid for 75 minutes at the ambient temperature of 75 DEG C under the ultrasonic wave effect with the power of 250W, filtering and extracting the feed liquid after ultrasonic wave extraction, and then drying in vacuum to obtain the flavonoids compounds. The invention has the advantages of simple extraction method, lower extraction requirement, low extraction cost, high extraction efficiency, and the like and is an ideal extraction method beyond the traditional thought.
Description
Technical field
The present invention relates to flavonoid compound extractive technique field, the extracting method of flavonoid compound in particularly a kind of asparagus and the tankage thereof.
Background technology
Asparagus is because meat is delicious bright and be of high nutritive value, and also has certain pharmaceutical use, therefore liked by everybody, contain abundant flavonoids in the asparagus, this flavonoid compound has multiple physiologically active and pharmacological actions such as Green Tea Extract, anti-oxidant, anticancer, antibiotic, antiviral, anti-inflammatory, antianaphylaxis, anti-diabetic complication, and have and reduce saturating property of blood vessel and brittle effect, treatment of diseases such as the mankind's tumour, aging, vascular disease and prevention there are important meaning.
The extracting method of the flavonoid compound in this asparagus of the prior art has very many, the Chinese patent publication number is the patent of invention of CN1821237A as has been publicly, what this patent adopted is the extracting method of supercritical carbon dioxide process, adopts this method to exist shortcomings such as requirement condition height, extraction cost height.
Summary of the invention
Be to solve the existing defective of extracting method and the problem of flavonoid compound in the prior art asparagus, the extracting method of flavonoid compound in a kind of asparagus of adopting biological enzymolysis and ultrasonic method and the tankage thereof is provided.
The technical scheme that the present invention is adopted for its technical problem of solution is: the technical process of the extracting method of flavonoid compound is in asparagus of the present invention and the tankage thereof: after asparagus and tankage thereof are cleaned, placing temperature is 60～70 ℃ down oven dry 3～4 hours of environment, take out and pulverize, cross 100 mesh sieves; By solid-liquid ratio is to add entry at 1: 10 to mix, and then adds the cellulase of asparagus opaque amount 5%, is 50 ℃ in envrionment temperature, and the pH value is to carry out enzymolysis 100 minutes under 5.5 the condition; Add ethanol again, making the shared concentration of ethanol is 80%, and solid-liquid ratio is 1: 100; Above-mentioned feed liquid is placed under the ultrasonic wave effect that power is 250W, and envrionment temperature is under 75 ℃ the condition, to extract 75 minutes; Feed liquid after the ultrasonic extraction is filtered extraction, carry out vacuum-drying then, promptly obtain flavonoid compound.
The invention has the beneficial effects as follows: compared with prior art, the extracting method of flavonoid compound adopts the ultrasonic wave principle in asparagus of the present invention and the tankage thereof, and under the effect of cellulase, carry out catalyzed reaction, it is simple to have extracting method, it is lower to extract requirement, extraction cost is low, and the extraction efficiency advantages of higher, is a kind of desirable extracting method of breaking through traditional thinking.
Embodiment
The technical process of the extracting method of flavonoid compound is in asparagus of the present invention and the tankage thereof:
1, asparagus and tankage thereof are cleaned after, placing temperature is 60～70 ℃ environment oven dry 3～4 hours down, takes out and pulverizes, and crosses 100 mesh sieves, promptly obtains the asparagus powder.
2, in the asparagus powder, be to add entry at 1: 10 to mix by solid-liquid ratio, solid-liquid ratio is 1: the 10 i.e. water of adding 10mL in per 1 gram asparagus powder, and then in mixture, add the cellulase of asparagus opaque amount 5%, the cellulase that promptly in the asparagus powder of per 100 grams, adds 5 grams, making mixture is 50 ℃ in envrionment temperature, and the pH value is to carry out enzymolysis 100 minutes under 5.5 the condition.
3, add ethanol again in mixture, making the shared concentration of ethanol is 80%, and promptly the concentration of the shared mixture of ethanol is 80%, and solid-liquid ratio is 1: 100, has the liquid of 100mL in promptly per 1 gram asparagus powder.
4, above-mentioned material liquid mixture is placed under the ultrasonic wave, extracts under the ultrasonic wave effect, when hyperacoustic power was 250W, envrionment temperature was under 75 ℃ the condition, need extract 75 minutes.
5, the feed liquid after the ultrasonic extraction is filtered extraction, carry out vacuum-drying then, promptly obtain flavonoid compound.
Can extract flavonoid compound in asparagus and the tankage thereof by above-mentioned technical process, the extracting method of this employing ultrasonic wave principle, it is simple to have an extracting method, it is lower to extract requirement, extraction cost is low, and the extraction efficiency advantages of higher, is a kind of brand-new extraction method.
, but should be understood that under the situation that does not break away from relevant protection domain defined by the claims that those skilled in the art can make change and/or revise according to embodiments of the invention describing the present invention property and nonrestrictive description.
Claims (3)
1. the extracting method of flavonoid compound in asparagus and the tankage thereof is characterized in that technical process is as follows:
(1), asparagus and tankage thereof are cleaned after, placing temperature is 60～70 ℃ environment oven dry 3～4 hours down, takes out and pulverizes, and crosses 100 mesh sieves;
(2), in the asparagus powder of the first step process gained, be to add entry at 1: 10 to mix by solid-liquid ratio, and then add the cellulase of asparagus opaque amount 5%, carried out enzymolysis 100 minutes;
(3), again add ethanol, making the shared concentration of ethanol is 80%, and solid-liquid ratio is 1: 100;
(4), above-mentioned feed liquid is placed under the ultrasonic wave, under the ultrasonic wave effect, extract;
(5), with the feed liquid after the ultrasonic extraction, filter extraction, carry out vacuum-drying then, promptly obtain flavonoid compound.
2. the extracting method of flavonoid compound is characterized in that in asparagus according to claim 1 and the tankage thereof, and described cellulase hydrolysis is 50 ℃ in envrionment temperature, and the pH value is to carry out under 5.5 the condition.
3. the extracting method of flavonoid compound is characterized in that in asparagus according to claim 1 and the tankage thereof, and described ultrasonic wave acts on the ultrasonic wave that power is 250W, and envrionment temperature is under 75 ℃ the condition, to extract 75 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010522492 CN101993902A (en) | 2010-10-27 | 2010-10-27 | Method for extracting flavonoids compounds from asparagus and leftovers thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010522492 CN101993902A (en) | 2010-10-27 | 2010-10-27 | Method for extracting flavonoids compounds from asparagus and leftovers thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101993902A true CN101993902A (en) | 2011-03-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN 201010522492 Pending CN101993902A (en) | 2010-10-27 | 2010-10-27 | Method for extracting flavonoids compounds from asparagus and leftovers thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104939242A (en) * | 2015-07-08 | 2015-09-30 | 浙江黛君生物医药科技有限公司 | Comprehensive utilization method of asparagus |
| CN112915154A (en) * | 2021-04-25 | 2021-06-08 | 四川工大西南食品研究有限责任公司 | Preparation method of asparagus flavone in asparagus leftovers |
| CN113425749A (en) * | 2021-06-29 | 2021-09-24 | 安徽仁之堂药业股份有限公司 | Utilization method of leftovers in processing of traditional Chinese medicine decoction pieces |
| CN114982792A (en) * | 2022-06-17 | 2022-09-02 | 常熟理工学院 | Extraction process, antibacterial activity and application of total flavonoids in agricultural wastes |
| CN116350722A (en) * | 2023-04-10 | 2023-06-30 | 河北科技师范学院 | Method for extracting flavone from asparagus by using eutectic solvent |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1821237A (en) * | 2006-03-14 | 2006-08-23 | 扬州大学 | Method for extracting flavone compound asparagus and its leftover |
| CN1973888A (en) * | 2006-12-11 | 2007-06-06 | 陕西科技大学 | Process of extracting rutin and other flavone matters from asparagus |
| CN101029320A (en) * | 2007-04-09 | 2007-09-05 | 重庆工学院 | Method for extracting bean mill cake isoflavone by cellulase synergistic supersonic wave |
| CN101406504A (en) * | 2008-11-24 | 2009-04-15 | 浙江大学 | Method for extracting flavone substances in jumi |
| CN101524458A (en) * | 2009-04-28 | 2009-09-09 | 湖南湘泉制药有限公司 | Extract of smilax glabra saponins and flavonoid and preparation method thereof |
-
2010
- 2010-10-27 CN CN 201010522492 patent/CN101993902A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1821237A (en) * | 2006-03-14 | 2006-08-23 | 扬州大学 | Method for extracting flavone compound asparagus and its leftover |
| CN1973888A (en) * | 2006-12-11 | 2007-06-06 | 陕西科技大学 | Process of extracting rutin and other flavone matters from asparagus |
| CN101029320A (en) * | 2007-04-09 | 2007-09-05 | 重庆工学院 | Method for extracting bean mill cake isoflavone by cellulase synergistic supersonic wave |
| CN101406504A (en) * | 2008-11-24 | 2009-04-15 | 浙江大学 | Method for extracting flavone substances in jumi |
| CN101524458A (en) * | 2009-04-28 | 2009-09-09 | 湖南湘泉制药有限公司 | Extract of smilax glabra saponins and flavonoid and preparation method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104939242A (en) * | 2015-07-08 | 2015-09-30 | 浙江黛君生物医药科技有限公司 | Comprehensive utilization method of asparagus |
| CN112915154A (en) * | 2021-04-25 | 2021-06-08 | 四川工大西南食品研究有限责任公司 | Preparation method of asparagus flavone in asparagus leftovers |
| CN113425749A (en) * | 2021-06-29 | 2021-09-24 | 安徽仁之堂药业股份有限公司 | Utilization method of leftovers in processing of traditional Chinese medicine decoction pieces |
| CN114982792A (en) * | 2022-06-17 | 2022-09-02 | 常熟理工学院 | Extraction process, antibacterial activity and application of total flavonoids in agricultural wastes |
| CN114982792B (en) * | 2022-06-17 | 2024-02-09 | 常熟理工学院 | Extraction process, bacteriostasis and application of total flavonoids in agricultural wastes |
| CN116350722A (en) * | 2023-04-10 | 2023-06-30 | 河北科技师范学院 | Method for extracting flavone from asparagus by using eutectic solvent |
| CN116350722B (en) * | 2023-04-10 | 2024-05-14 | 河北科技师范学院 | Method for extracting flavone from asparagus by using eutectic solvent |
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Application publication date: 20110330 |