CN103989748A - Method for extracting lotus seedpod procyanidins from lotus seedpod through full-low-temperature process - Google Patents
Method for extracting lotus seedpod procyanidins from lotus seedpod through full-low-temperature process Download PDFInfo
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- CN103989748A CN103989748A CN201410245924.8A CN201410245924A CN103989748A CN 103989748 A CN103989748 A CN 103989748A CN 201410245924 A CN201410245924 A CN 201410245924A CN 103989748 A CN103989748 A CN 103989748A
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Abstract
The invention discloses a method for extracting lotus seedpod procyanidins from lotus seedpod through the full-low-temperature process. The method is characterized in that a low-temperature flash extraction device, a low-temperature negative-pressure alcohol recovering and concentrating device and a low-temperature negative-pressure ultrasonic drying device are used in match to extract lotus seedpod procyanidins from lotus seedpod. According to the method, the whole process is performed under low temperature (below 45 DEG C), the resin adsorption-based production process is avoided, the extracted lotus seedpod procyanidins is high in content and high in quality, and the demands on development of lotus seedpod procyanidins health foods and drugs are met.
Description
Technical field
The present invention relates to a kind of plant component extraction method, specifically a kind of full chilling process extracts the method for LSPC in Receptaculum Nelumbinis.
Background technology
Procyanidin compounds causes countries in the world scientist's extensive concern with its significant antioxidant effect and distinctive biological activity and pharmacological action, and wherein grape pip procyanidin is the focus of research for a long time always.The research of grape pip procyanidin is further goed deep into perfect in, various countries scientist has turned to attention other plant.Extensively, according to interrelated data, within 1999, national Annual planting area has just surpassed 40000hm to the plantation of Lian China
2.Rhizoma Nelumbinis and Semen Nelumbinis are the food that people like, Folium Nelumbinis is the medicine-food two-purpose kind that Ministry of Public Health is assert, and Receptaculum Nelumbinis is taken as garbage and abandons in a large number.In < < Compendium of Materia Medica > >, record, Flos Nelumbinis, Semen Nelumbinis, Testa Nelumbinis, Receptaculum Nelumbinis, Stamen Nelumbinis, Plumula Nelumbinis, Folium Nelumbinis, Petiolus nelumbinis, Nodus Nelumbinis Rhizomatis etc. all must be used.In Receptaculum Nelumbinis, containing the multiple bioactive ingredients such as protein, fat, saccharide, crude fibre, carotene, dimension B, Vc, nicotinic acid and micro-nelumbine, is Chinese medicine material, has the effect of the silt that disappears, hemostasis damp eliminating.Modern bioactivity research shows, in Receptaculum Nelumbinis, be rich in LSPC (be called for short LSPC), multiple biological activity and the pharmacological actions such as LSPC has stronger removing free radical and inhibition of lipid peroxidation, resists myocardial ischemia, regulates blood fat, anticancer growth and radioprotective.Receptaculum Nelumbinis further confirms with the comparative study of grape pip procyanidin extract, and the two is to DPPH
. free radical and OH
. the removing ability of free radical is without marked difference, and its total reducing power is also roughly suitable.For this reason, take Receptaculum Nelumbinis as raw material, extract LSPC industrialization, developing its health food and medicine will have larger economy and social benefit.
At present, some institutes have carried out LSPC extraction and the research of pharmacological experiment chamber, and having no the report that full chilling process extracts LSPC industrialization, the flash extractive technique of low temperature and the ultrasonic dry technology of low-temperature negative-pressure extract industrialization for LSPC and still belong to blank.
Summary of the invention
The object of this invention is to provide a kind of method that full chilling process extracts LSPC in Receptaculum Nelumbinis, the method is combined the flash extractive technique of use low temperature, low-temperature negative-pressure recovery ethanol and concentration technique, the ultrasonic dry technology of low-temperature negative-pressure extracts LSPC extract in Receptaculum Nelumbinis, evade resin absorption production technology and obtain the high-quality LSPC of high-load, met the needs of procyanidin health food and drug development.
Object of the present invention is achieved through the following technical solutions:
In a kind of full chilling process, extract the method for LSPC extract in Receptaculum Nelumbinis, it is characterized in that: the method is combined the LSPC using in the flash extraction element of low temperature, low-temperature negative-pressure ethanol recovery enrichment facility, the ultrasonic drying device extraction of low-temperature negative-pressure Receptaculum Nelumbinis, specifically comprises the steps:
1) Receptaculum Nelumbinis after fragmentation is made to solvent with edible ethanol, below 45 ℃, under cryogenic conditions, extracting, obtain LSPC ethanol extract; Wherein, the mass ratio of Receptaculum Nelumbinis and edible ethanol is 1:10-10:1.Can adopt 45 ℃ of following low temperature flash extracters to extract, obtain LSPC ethanol extract.
2) under low temperature below 45 ℃ ,-0.099mpa condition of negative pressure, reclaim ethanol concentrated extracting solution, obtain LSPC and extract extractum; Can adopt 45 ℃ of following low temperature ,-0.099mpa negative pressure alcohol recovering devices to reclaim ethanol concentrated extracting solution, obtain LSPC and extract extractum.
3, under low temperature below 45 ℃ ,-0.099mpa condition of negative pressure, LSPC extract dry is become to LSPC extract powder, moisture is lower than 6%, and its LSPC content reaches more than 45%.Can adopt 45 ℃ of following low temperature ,-0.099mpa negative pressure ultrasound drying devices that LSPC extract dry is become to LSPC extract powder, in ultrasonic drying device, be 4-5 hour drying time.
The present invention makees solvent by the Receptaculum Nelumbinis after fragmentation with edible ethanol, adopts low temperature (45 ℃ following) flash extracter to extract, and obtains LSPC ethanol extract.Adopt low temperature (45 ℃ following) negative pressure (0.099mpa) alcohol recovering device to reclaim ethanol concentrated extracting solution, obtain LSPC and extract extractum.Adopt low temperature (45 ℃ following) the ultrasonic drying device of negative pressure (0.099mpa) that LSPC extract dry is become to LSPC extract powder (moisture is lower than 6%), its LSPC content can reach more than 45%.
Useful result of the present invention is as follows:
1, the procyanidin method of extracting in plant with circumfluence method is compared, and not only temperature is low, and flash extraction has been shortened extraction time greatly.For this reason, the reservation of highly significant more bioactive ingredients, greatly reduce production cost.
2, the ultrasonic dry product quality obtaining of low-temperature negative-pressure of the present invention is completely suitable with cryodesiccated quality, and its production cost is lower than lyophilization production cost, and can form serial automatic production line production, has greatly improved production efficiency.
3, the procyanidin in LSPC extract of the present invention is greater than 45%, met the requirement of health food and pharmaceutical production completely, and without resin absorption refining crude extract, reduced undoubtedly production cost, what is more important has been evaded the product toxicity risk that resin may come off and bring completely, has guaranteed the safety of product.
The specific embodiment
Embodiment 1
Full chilling process extracts a method for LSPC in Receptaculum Nelumbinis, combines and uses the flash extraction element of low temperature, low-temperature negative-pressure ethanol to reclaim the LSPC in enrichment facility, the ultrasonic drying device extraction of low-temperature negative-pressure Receptaculum Nelumbinis, comprises the steps:
1) be ground into 40-60 object Receptaculum Nelumbinis 3kg, by 35L, 40% edible ethanol solution soaking, after 2 hours, at 40 ℃, through flash extracter, extract 20min.300 orders filter to obtain LSPC extracting solution 21kg.
2) LSPC extracting solution is adopted low temperature (45 ℃ following) negative pressure (0.099mpa) retracting device reclaim ethanol concentrated extracting solution, obtain LSPC and extract extractum 0.7kg.
3) LSPC extractum is dried to 4 hours in the ultrasonic drying device of low temperature (below 45 ℃) negative pressure (0.099mpa), obtains LSPC extract powder 0.3kg, its moisture is lower than 6%, and procyanidin content can reach 46%.
Embodiment 2
Full chilling process extracts a method for LSPC in Receptaculum Nelumbinis, comprises the steps:
1) be ground into 40-60 object Receptaculum Nelumbinis 5kg, by 60L, 60% edible ethanol solution soaking, after 2 hours, at 40 ℃, through flash extracter, extract 20min.300 orders filter to obtain LSPC extracting solution 36kg.
2) LSPC extracting solution is adopted low temperature (45 ℃ following) negative pressure (0.099mpa) retracting device reclaim ethanol concentrated extracting solution, obtain LSPC and extract extractum 1.1kg.
3) LSPC extractum is dried to 4 hours in the ultrasonic drying device of low temperature (below 45 ℃) negative pressure (0.099mpa), obtains LSPC extract powder 0.52kg, its moisture is lower than 6%, and procyanidin content can reach 46.5%.
Claims (5)
1. in a full chilling process, extract the method for LSPC extract in Receptaculum Nelumbinis, it is characterized in that: the method is combined the LSPC using in the flash extraction element of low temperature, low-temperature negative-pressure ethanol recovery enrichment facility, the ultrasonic drying device extraction of low-temperature negative-pressure Receptaculum Nelumbinis, specifically comprises the steps:
1) Receptaculum Nelumbinis after fragmentation is made to solvent with edible ethanol, below 45 ℃, under cryogenic conditions, extracting, obtain LSPC ethanol extract; Wherein, the mass ratio of Receptaculum Nelumbinis and edible ethanol is 1:10-10:1;
2) under low temperature below 45 ℃ ,-0.099mpa condition of negative pressure, reclaim ethanol concentrated extracting solution, obtain LSPC and extract extractum;
Under low temperature below 45 ℃ ,-0.099mpa condition of negative pressure, LSPC extract dry is become to LSPC extract powder, moisture is lower than 6%, and its LSPC content reaches more than 45%.
2. in full chilling process according to claim 1, extract the method for LSPC extract in Receptaculum Nelumbinis, it is characterized in that: in step 1), adopt 45 ℃ of following low temperature flash extracters to extract, obtain LSPC ethanol extract.
3. in full chilling process according to claim 1, extract the method for LSPC extract in Receptaculum Nelumbinis, it is characterized in that: in step 1), Receptaculum Nelumbinis is ground into 40-60 orders.
4. in full chilling process according to claim 1, extract the method for LSPC extract in Receptaculum Nelumbinis, it is characterized in that: step 2) in, adopt 45 ℃ of following low temperature ,-0.099mpa negative pressure alcohol recovering devices to reclaim ethanol concentrated extracting solution, obtain LSPC and extract extractum.
5. in full chilling process according to claim 1, extract the method for LSPC extract in Receptaculum Nelumbinis, it is characterized in that: in step 3), adopt 45 ℃ of following low temperature ,-0.099mpa negative pressure ultrasound drying devices that LSPC extract dry is become to LSPC extract powder, in ultrasonic drying device, be 4-5 hour drying time.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105524036A (en) * | 2016-01-12 | 2016-04-27 | 北京宝得瑞健康产业有限公司 | High-efficiency low-consumption grape seed procyanidine extraction method |
| CN106674185A (en) * | 2016-11-30 | 2017-05-17 | 辽宁大学 | Method for extracting procyanidine from fruit or pomace of aronia melanocarpa elliot |
| CN109091409A (en) * | 2018-10-25 | 2018-12-28 | 福建农林大学 | A kind of preparation method of passionflower anthocyanidin and ovalbumin peptide facial mask dry powder |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1506361A (en) * | 2002-12-06 | 2004-06-23 | 沈阳中科药业公司 | Low temperature proanthocyanidin extracting process from grape seed |
| CN103304532A (en) * | 2013-06-19 | 2013-09-18 | 江西安正利康生命科技有限公司 | Method for extracting procyanidine from receptaculum nelumbinis |
| CN103497170A (en) * | 2013-09-12 | 2014-01-08 | 邹元生 | Method for extracting procyanidine in receptaculum nelumbinis in full low temperature process |
| CN103694294A (en) * | 2014-01-10 | 2014-04-02 | 河南智晶生物科技有限公司 | Method for extracting baicalin by use of flash extraction technology |
-
2014
- 2014-06-05 CN CN201410245924.8A patent/CN103989748A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1506361A (en) * | 2002-12-06 | 2004-06-23 | 沈阳中科药业公司 | Low temperature proanthocyanidin extracting process from grape seed |
| CN103304532A (en) * | 2013-06-19 | 2013-09-18 | 江西安正利康生命科技有限公司 | Method for extracting procyanidine from receptaculum nelumbinis |
| CN103497170A (en) * | 2013-09-12 | 2014-01-08 | 邹元生 | Method for extracting procyanidine in receptaculum nelumbinis in full low temperature process |
| CN103694294A (en) * | 2014-01-10 | 2014-04-02 | 河南智晶生物科技有限公司 | Method for extracting baicalin by use of flash extraction technology |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105524036A (en) * | 2016-01-12 | 2016-04-27 | 北京宝得瑞健康产业有限公司 | High-efficiency low-consumption grape seed procyanidine extraction method |
| CN106674185A (en) * | 2016-11-30 | 2017-05-17 | 辽宁大学 | Method for extracting procyanidine from fruit or pomace of aronia melanocarpa elliot |
| CN109091409A (en) * | 2018-10-25 | 2018-12-28 | 福建农林大学 | A kind of preparation method of passionflower anthocyanidin and ovalbumin peptide facial mask dry powder |
| CN109091409B (en) * | 2018-10-25 | 2021-09-21 | 福建农林大学 | Preparation method of passion flower anthocyanin and egg albumin peptide mask dry powder |
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Application publication date: 20140820 |