CN104623095A - Extraction method for FructusLycii active ingredient - Google Patents
Extraction method for FructusLycii active ingredient Download PDFInfo
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- CN104623095A CN104623095A CN201510071553.0A CN201510071553A CN104623095A CN 104623095 A CN104623095 A CN 104623095A CN 201510071553 A CN201510071553 A CN 201510071553A CN 104623095 A CN104623095 A CN 104623095A
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Abstract
The invention discloses an extraction method for fructuslycii active ingredient. the extraction method specifically comprises the following steps: drying fructuslycii, grinding, adding ethanol solution, transferring into a microwave extractor for extraction; transferring into an extraction kettle and performing supercritical carbon dioxide extraction, regulating the extraction pressure and carbon dioxide flow rate, carrying out two-stage reduced-pressure separation to an extract, regulating the pressure and temperature of a first-stage separation kettle and a second-stage reaction kettle, collecting a product, transferring a freeze dryer and drying to obtain lycium barbarum polysaccharide and flavone. According to the method, the extraction technology is simple, the extraction efficiency is high, the product has no residual solvent substances, and is safe and feasible, the extracted polysaccharide and flavone substances has the efficacies of resisting virus and lowering blood sugar.
Description
Technical field
The present invention relates to active component extractive technique field, especially a kind of extracting method of matrimony vine active composition.
Background technology
Fructus Lycii is the mature fruit of matrimony vine of solanaceae plant, has various health care functions, and be the medicine-food two-purpose food of Ministry of Public Health approval, proper amount of edible is good for health.Modern pharmacology research confirms, lycium barbarum has raising immunity of organism, defying age, antitumor, replenishing vital essence to improve eyesight, reduces many-sided pharmacological actions such as blood fat.Chinese scholars starts to study the chemical composition of lycium barbarum very early, show that the chemical composition of lycium barbarum is mainly lycium barbarum polysaccharide, flavone and glycoside thereof, coumarin, carotenoid, aminoacid etc.
The extracting method of existing matrimony vine active composition mainly with ethanol, ether or ethyl acetate for Extraction solvent, mainly comprise direct decocting method, infusion process, heat reflow method etc., various extracting method is still traditional water extract-alcohol precipitation or alcohol extracting-water precipitating, a large amount of precipitation is produced in subsequent treatment, active component is not only made to lose in a large number, return the technological operations such as filtration and bring larger difficulty, time consumption and energy consumption, extraction efficiency is low.
Summary of the invention
The object of this invention is to provide a kind of extracting method of matrimony vine active composition, this extracting method can solve the inefficient problem of existing matrimony vine active constituents extraction.
In order to solve the problem, the technical solution used in the present invention is:
The extracting method of matrimony vine active composition of the present invention comprises the following steps:
A, by Fructus Lycii dry, pulverize, obtain Fructus Lycii powder, add alcoholic solution, proceed in microwave extraction instrument and extract;
B, proceed in extraction kettle and carry out supercritical carbon dioxide extraction, regulate extracting pressure and carbon dioxide flow rate, extract carries out two-stage decompression separation, regulates the pressure and temperature of flash trapping stage still and secondary response still, collects product, for subsequent use;
C, above-mentioned product is proceeded to freezer dryer carry out drying, obtain pressed powder;
Wherein, described matrimony vine active composition refers to lycium barbarum polysaccharide and flavone.
In technique scheme, scheme can also be more specifically: step B extracting pressure is 23 MPa ~ 60 MPas, and flash trapping stage still pressure is 10 MPa ~ 17 MPas, and the second-order separation still pressure is 5 MPa ~ 8 MPas.
Further, step B carbon dioxide flow rate is 15 kgs/hr ~ 23 kgs/hr.
Further, step B extraction temperature is 25 DEG C ~ 35 DEG C.
Further, step A Extracting temperature is 45 DEG C ~ 55 DEG C, and extraction time is 25 minutes ~ 40 minutes.
Owing to have employed technique scheme, the present invention compared with prior art has following beneficial effect:
1, the extracting method of matrimony vine active composition of the present invention, first microwave radiation exaraction is adopted the polysaccharide in Fructus Lycii and flavone to be carried out just carrying, then supercritical carbon dioxide extraction method is adopted, better can not only keep the effective ingredient of extract, extraction efficiency is high, and the vehicle substance of product noresidue, thus prevent the existence of harmful thing in leaching process and the pollution to environment.
2, extraction process of the present invention is simple, once can obtain main active, and safety is easy, and the polysaccharide extracted and Flavonoid substances have effect of antiviral, blood sugar lowering.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
The extracting method of the present embodiment matrimony vine active composition is: dried by 1 kilogram of Fructus Lycii, and pulverize, obtain Fructus Lycii powder, add 5 kilograms of alcoholic solution, proceed in microwave extraction instrument and extract, set temperature is 45 DEG C, and extraction time is 40 minutes; Proceed in extraction kettle and carry out supercritical carbon dioxide extraction, extracting pressure is regulated to be 23 MPas, extraction temperature is 35 DEG C, regulation of carbon dioxide flow velocity is 15 kgs/hr, and extract is carried out two-stage decompression separation, and arranging flash trapping stage still pressure is 17 MPas, the pressure of secondary response still is 8 MPas, collect products therefrom, proceed to freezer dryer and carry out drying, obtain lycium barbarum polysaccharide and flavone.
Embodiment 2
The extracting method of the present embodiment matrimony vine active composition is: dried by 10 kilograms of Fructus Lycii, and pulverize, obtain Fructus Lycii powder, add 80 kilograms of alcoholic solution, proceed in microwave extraction instrument and extract, set temperature is 55 DEG C, and extraction time is 30 minutes; Proceed in extraction kettle and carry out supercritical carbon dioxide extraction, extracting pressure is regulated to be 45 MPas, extraction temperature is 30 DEG C, regulation of carbon dioxide flow velocity is 20 kgs/hr, and extract is carried out two-stage decompression separation, and arranging flash trapping stage still pressure is 14 MPas, the pressure of secondary response still is 6.5 MPas, collect products therefrom, proceed to freezer dryer and carry out drying, obtain lycium barbarum polysaccharide and flavone.
Embodiment 3
The extracting method of the present embodiment matrimony vine active composition is: dried by 20 kilograms of Fructus Lycii, and pulverize, obtain Fructus Lycii powder, add 120 kilograms of alcoholic solution, proceed in microwave extraction instrument and extract, set temperature is 50 DEG C, and extraction time is 25 minutes; Proceed in extraction kettle and carry out supercritical carbon dioxide extraction, extracting pressure is regulated to be 60 MPas, extraction temperature is 25 DEG C, regulation of carbon dioxide flow velocity is 23 kgs/hr, and extract is carried out two-stage decompression separation, and arranging flash trapping stage still pressure is 10 MPas, the pressure of secondary response still is 5 MPas, collect products therefrom, proceed to freezer dryer and carry out drying, obtain lycium barbarum polysaccharide and flavone.
Claims (5)
1. an extracting method for matrimony vine active composition, is characterized in that comprising the following steps:
A, by Fructus Lycii dry, pulverize, obtain Fructus Lycii powder, add alcoholic solution, proceed in microwave extraction instrument and extract;
B, proceed in extraction kettle and carry out supercritical carbon dioxide extraction, regulate extracting pressure and carbon dioxide flow rate, extract carries out two-stage decompression separation, regulates the pressure and temperature of flash trapping stage still and secondary response still, collects product, for subsequent use;
C, above-mentioned product is proceeded to freezer dryer carry out drying, obtain pressed powder;
Wherein, described matrimony vine active composition refers to lycium barbarum polysaccharide and flavone.
2. the extracting method of matrimony vine active composition according to claim 1, it is characterized in that step B extracting pressure is 23 MPa ~ 60 MPas, flash trapping stage still pressure is 10 MPa ~ 17 MPas, and the second-order separation still pressure is 5 MPa ~ 8 MPas.
3. the extracting method of matrimony vine active composition according to claim 1, is characterized in that step B carbon dioxide flow rate is 15 kgs/hr ~ 23 kgs/hr.
4. the extracting method of matrimony vine active composition according to claim 1, is characterized in that step B extraction temperature is 25 DEG C ~ 35 DEG C.
5. the extracting method of matrimony vine active composition according to claim 1, it is characterized in that step A Extracting temperature is 45 DEG C ~ 55 DEG C, extraction time is 25 minutes ~ 40 minutes.
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| CN201510071553.0A CN104623095A (en) | 2015-02-11 | 2015-02-11 | Extraction method for FructusLycii active ingredient |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106983148A (en) * | 2017-05-15 | 2017-07-28 | 中国科学院西北高原生物研究所 | A kind of wolfberry chewable tablet and preparation method thereof |
| CN107898868A (en) * | 2017-10-24 | 2018-04-13 | 中国科学院兰州化学物理研究所 | The method that separated in synchronization prepares matrimony vine red pigment, polysaccharides and matrimony vine flavones from matrimony vine |
| CN108392542A (en) * | 2018-05-08 | 2018-08-14 | 广西大学 | Flavonoids method in a kind of enzyme assisted microwave synthesis extraction matrimony vine |
| CN108785449A (en) * | 2018-06-15 | 2018-11-13 | 宁夏宝塔化工中心实验室(有限公司) | A kind of extraction process of matrimony vine flavones |
| CN108822229A (en) * | 2018-09-05 | 2018-11-16 | 杨鑫 | A kind of extracting method of lycium ruthenicum polysaccharide |
| CN109463503A (en) * | 2018-12-20 | 2019-03-15 | 株洲千金药业股份有限公司 | A kind of ampelopsis grossdentata leaf extract and its application in terms of health care product |
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| FR2068433A1 (en) * | 1969-11-18 | 1971-08-27 | Garcia Jose | Japanese medlar antihaemorrhoidal extracts |
| CN102988605A (en) * | 2012-11-15 | 2013-03-27 | 山西五台山沙棘制品有限公司 | Method for extracting lycium barbarum extract by supercritical CO2 |
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| FR2068433A1 (en) * | 1969-11-18 | 1971-08-27 | Garcia Jose | Japanese medlar antihaemorrhoidal extracts |
| CN102988605A (en) * | 2012-11-15 | 2013-03-27 | 山西五台山沙棘制品有限公司 | Method for extracting lycium barbarum extract by supercritical CO2 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106983148A (en) * | 2017-05-15 | 2017-07-28 | 中国科学院西北高原生物研究所 | A kind of wolfberry chewable tablet and preparation method thereof |
| CN107898868A (en) * | 2017-10-24 | 2018-04-13 | 中国科学院兰州化学物理研究所 | The method that separated in synchronization prepares matrimony vine red pigment, polysaccharides and matrimony vine flavones from matrimony vine |
| CN107898868B (en) * | 2017-10-24 | 2021-07-13 | 宁夏中杞生物科技有限公司 | Method for synchronously separating and preparing lycium erythrophyll, lycium barbarum polysaccharide and lycium barbarum flavone from lycium barbarum |
| CN108392542A (en) * | 2018-05-08 | 2018-08-14 | 广西大学 | Flavonoids method in a kind of enzyme assisted microwave synthesis extraction matrimony vine |
| CN108785449A (en) * | 2018-06-15 | 2018-11-13 | 宁夏宝塔化工中心实验室(有限公司) | A kind of extraction process of matrimony vine flavones |
| CN108822229A (en) * | 2018-09-05 | 2018-11-16 | 杨鑫 | A kind of extracting method of lycium ruthenicum polysaccharide |
| CN108822229B (en) * | 2018-09-05 | 2020-08-14 | 山东新稀宝股份有限公司 | Extraction method of lycium ruthenicum polysaccharide |
| CN109463503A (en) * | 2018-12-20 | 2019-03-15 | 株洲千金药业股份有限公司 | A kind of ampelopsis grossdentata leaf extract and its application in terms of health care product |
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Application publication date: 20150520 |