CN104666427A - Method for extracting main active components of folium mori - Google Patents
Method for extracting main active components of folium mori Download PDFInfo
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- CN104666427A CN104666427A CN201510071502.8A CN201510071502A CN104666427A CN 104666427 A CN104666427 A CN 104666427A CN 201510071502 A CN201510071502 A CN 201510071502A CN 104666427 A CN104666427 A CN 104666427A
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Abstract
The invention discloses a method for extracting main active components of folium mori. The method comprises the following specific steps: drying and crushing folium mori, and filtering through a molecular sieve to obtain folium mori powder; transferring the folium mori powder to an extraction tank, carrying out supercritical carbon dioxide extraction, adjusting the extraction pressure and the flow rate of carbon dioxide, carrying out two-stage decompression separation on the extract, and adjusting the pressure and the temperatures of a primary separation kettle and a secondary separation kettle; collecting a product, and extracting with an ethanol solution; and extracting with ethanol water containing ammonia, heating, concentrating and drying to obtain flavone and alkaloid. A supercritical carbon dioxide extraction technique is mainly adopted; the extraction technology is simple; the main active component can be obtained once; the method is safe and feasible; the product is free of a residual solvent material; and the efficiency is improved.
Description
Technical field
The present invention relates to active component extractive technique field, especially a kind of extracting method of main active components of mulberry leaf.
Background technology
Folium Mori, as the Chinese crude drug of medicine-food two-purpose, are usually used in and other Chinese prescription partner treatment diabetes.Polyhydroxylated alkaloid in the Folium Mori that first Japanese scholars finds have with saccharide like structure, can the activity of disaccharide-hydrolysing enzymes-alpha-glucosidase in reversible inhibition small intestinal, reduce the glucose amount entering blood, reduce post-prandial glycemia.This Alkaloid has the structure similar with the acarbose of current Clinical practice and the mechanism of action.Meanwhile, research proves, alpha-glucosidase inhibitor also has obesity, antiviral therapeutical effect.In addition, lot of documents is had to report in Folium Mori also containing the flavonoid active ingredient with effect of lowering blood sugar.
At present, the mode that in Folium Mori, alkaloid and flavone component mainly adopt macroporous adsorbent resin and cation exchange resin to use continuously is extracted, there is certain deficiency in the method: namely Folium Mori extracts active ingredients utilizes not exclusively, or is not separated whole principle active component by primary sorption.
Summary of the invention
The object of this invention is to provide a kind of extracting method of main active components of mulberry leaf, this extracting method can solve the low problem of alkaloid and flavonoid substance extraction ratio in Folium Mori.
In order to solve the problem, the technical solution used in the present invention is:
The extracting method of this main active components of mulberry leaf comprises the following steps:
A, by Folium Mori dry pulverize, cross molecular sieve, obtain Mulberry Leaf, for subsequent use;
B, to be proceeded in extractor by described Mulberry Leaf and carry out supercritical carbon dioxide extraction, regulate extracting pressure and carbon dioxide flow rate, extract carries out two-stage decompression separation, regulates the pressure and temperature of flash trapping stage still and secondary response still, collects product, for subsequent use;
C, be the ratio of 1:3 ~ 5 according to volume ratio, product alcoholic solution step B obtained extracts, then is the ratio of 1:2 ~ 5 according to volume ratio, extracts with the ethanol water containing ammonia, and heating is concentrated, dries, obtains flavone and alkaloid.
In technique scheme, scheme can also be more specifically: step B extracting pressure is 25 MPa ~ 50 MPas, and flash trapping stage still pressure is 13 MPa ~ 20 MPas, and the second-order separation still pressure is 7 MPa ~ 10 MPas.
Further, step B carbon dioxide flow rate is 15 kgs/hr ~ 23 kgs/hr.
Further, step B extraction temperature is 25 DEG C ~ 35 DEG C.
Owing to have employed technique scheme, the present invention compared with prior art has following beneficial effect:
1, the present invention mainly adopts supercritical carbon dioxide extraction, on the one hand product noresidue vehicle substance, thus prevents the existence of harmful thing in leaching process and the pollution to environment, better can keep the effective ingredient of extract on the other hand.
2, extraction process of the present invention is simple, once can obtain main active, and safety is easy, raises the efficiency height.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
1.18 kilograms of Folium Mori are dried and pulverizes, cross molecular sieve, obtain 1 kilogram of Mulberry Leaf, proceed in extractor and carry out supercritical carbon dioxide extraction, arranging extraction temperature is 25 DEG C ~ 35 DEG C, extracting pressure is regulated to be 25 MPa ~ 50 MPas, carbon dioxide flow rate 15 kgs/hr ~ 23 kgs/hr, extract is carried out two-stage decompression separation, flash trapping stage still pressure is regulated to be 13 MPa ~ 20 MPas, the pressure of secondary response still is 7 MPa ~ 10 MPas, collect product, add 0.6 liter of alcoholic solution to extract, add 0.4 liter of ethanol water containing ammonia again to extract, heating is concentrated, dry, obtain flavone and alkaloid.
Embodiment 2
11.3 kilograms of Folium Mori are dried and pulverizes, cross molecular sieve, obtain 10.1 kilograms of Mulberry Leafs, proceed in extractor and carry out supercritical carbon dioxide extraction, arranging extraction temperature is 25 DEG C ~ 35 DEG C, extracting pressure is regulated to be 25 MPa ~ 50 MPas, carbon dioxide flow rate 15 kgs/hr ~ 23 kgs/hr, extract is carried out two-stage decompression separation, flash trapping stage still pressure is regulated to be 13 MPa ~ 20 MPas, the pressure of secondary response still is 7 MPa ~ 10 MPas, collect product, add 8 liters of alcoholic solution to extract, add 7.5 liters of ethanol waters containing ammonia again to extract, heating is concentrated, dry, obtain flavone and alkaloid.
Embodiment 3
20 kilograms of Folium Mori are dried and pulverizes, cross molecular sieve, obtain 18.6 kilograms of Mulberry Leafs, proceed in extractor and carry out supercritical carbon dioxide extraction, arranging extraction temperature is 25 DEG C ~ 35 DEG C, extracting pressure is regulated to be 25 MPa ~ 50 MPas, carbon dioxide flow rate 15 kgs/hr ~ 23 kgs/hr, extract is carried out two-stage decompression separation, flash trapping stage still pressure is regulated to be 13 MPa ~ 20 MPas, the pressure of secondary response still is 7 MPa ~ 10 MPas, collect product, add 20 liters of alcoholic solution to extract, add 19 liters of ethanol waters containing ammonia again to extract, heating is concentrated, dry, obtain flavone and alkaloid.
Claims (4)
1. an extracting method for main active components of mulberry leaf, is characterized in that comprising the following steps:
A, by Folium Mori dry pulverize, cross molecular sieve, obtain Mulberry Leaf, for subsequent use;
B, to be proceeded in extractor by described Mulberry Leaf and carry out supercritical carbon dioxide extraction, regulate extracting pressure and carbon dioxide flow rate, extract carries out two-stage decompression separation, regulates the pressure and temperature of flash trapping stage still and secondary response still, collects product, for subsequent use;
C, be the ratio of 1:3 ~ 5 according to volume ratio, product alcoholic solution step B obtained extracts, then is the ratio of 1:2 ~ 5 according to volume ratio, extracts with the ethanol water containing ammonia, and heating is concentrated, dries, obtains flavone and alkaloid.
2. the extracting method of main active components of mulberry leaf according to claim 1, it is characterized in that step B extracting pressure is 25 MPa ~ 50 MPas, flash trapping stage still pressure is 13 MPa ~ 20 MPas, and the second-order separation still pressure is 7 MPa ~ 10 MPas.
3. the extracting method of main active components of mulberry leaf according to claim 1, is characterized in that step B carbon dioxide flow rate is 15 kgs/hr ~ 23 kgs/hr.
4. the extracting method of main active components of mulberry leaf according to claim 1, is characterized in that step B extraction temperature is 25 DEG C ~ 35 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510071502.8A CN104666427A (en) | 2015-02-11 | 2015-02-11 | Method for extracting main active components of folium mori |
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| CN201510071502.8A CN104666427A (en) | 2015-02-11 | 2015-02-11 | Method for extracting main active components of folium mori |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111636217A (en) * | 2020-06-23 | 2020-09-08 | 广东精英纺织服饰科技有限公司 | Textile dye with flame retardant function and preparation method thereof |
| WO2021214292A1 (en) | 2020-04-24 | 2021-10-28 | Société des Produits Nestlé S.A. | Composition comprising fibre and mulberry |
| WO2021214326A1 (en) | 2020-04-24 | 2021-10-28 | Société des Produits Nestlé S.A. | Use of mulberry extract for controlling postprandial glucose response |
| WO2023046713A1 (en) | 2021-09-21 | 2023-03-30 | Société des Produits Nestlé S.A. | Compositions and methods lowering glycemic response to improve sleep quality and/or subsequent behavioural outcomes |
| WO2023046719A2 (en) | 2021-09-21 | 2023-03-30 | Société des Produits Nestlé S.A. | Methods and devices for improving sleep quality and/or subsequent behavioural outcomes |
| WO2023046715A1 (en) | 2021-09-21 | 2023-03-30 | Société des Produits Nestlé S.A. | Use of mulberry extract for improving sleep quality and/or subsequent behavioural outcomes |
| WO2023165856A1 (en) | 2022-03-02 | 2023-09-07 | Société des Produits Nestlé S.A. | Use of a composition for managing postprandial glucose response and associated disorders |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104274534A (en) * | 2013-07-03 | 2015-01-14 | 周伯扬 | Preparation technology of total-component mulberry leaf extract |
-
2015
- 2015-02-11 CN CN201510071502.8A patent/CN104666427A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104274534A (en) * | 2013-07-03 | 2015-01-14 | 周伯扬 | Preparation technology of total-component mulberry leaf extract |
Non-Patent Citations (2)
| Title |
|---|
| 林英男等: "超临界CO2提取桑叶中总黄酮的工艺研究", 《齐鲁工业大学学报》 * |
| 王婴等: "桑叶生物碱提取工艺研究进展", 《广东化工》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021214292A1 (en) | 2020-04-24 | 2021-10-28 | Société des Produits Nestlé S.A. | Composition comprising fibre and mulberry |
| WO2021214326A1 (en) | 2020-04-24 | 2021-10-28 | Société des Produits Nestlé S.A. | Use of mulberry extract for controlling postprandial glucose response |
| CN111636217A (en) * | 2020-06-23 | 2020-09-08 | 广东精英纺织服饰科技有限公司 | Textile dye with flame retardant function and preparation method thereof |
| WO2023046713A1 (en) | 2021-09-21 | 2023-03-30 | Société des Produits Nestlé S.A. | Compositions and methods lowering glycemic response to improve sleep quality and/or subsequent behavioural outcomes |
| WO2023046719A2 (en) | 2021-09-21 | 2023-03-30 | Société des Produits Nestlé S.A. | Methods and devices for improving sleep quality and/or subsequent behavioural outcomes |
| WO2023046715A1 (en) | 2021-09-21 | 2023-03-30 | Société des Produits Nestlé S.A. | Use of mulberry extract for improving sleep quality and/or subsequent behavioural outcomes |
| WO2023165856A1 (en) | 2022-03-02 | 2023-09-07 | Société des Produits Nestlé S.A. | Use of a composition for managing postprandial glucose response and associated disorders |
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Application publication date: 20150603 |