CN109463503A - A kind of ampelopsis grossdentata leaf extract and its application in terms of health care product - Google Patents
A kind of ampelopsis grossdentata leaf extract and its application in terms of health care product Download PDFInfo
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- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
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Abstract
The invention discloses a kind of ampelopsis grossdentata leaf extract and its applications in terms of health care product.After the present invention is by active principle in microwave digestion ampelopsis grossdentata leaf, residual ampelopsis grossdentata leaf extract is further extracted by supercritical carbon dioxide extracting, dual extracting method obtains ampelopsis grossdentata leaf extract, greatly improve the recovery rate of dihydromyricetin in ampelopsis grossdentata leaf, technical foundation is provided for its preparation for being applied to health care product, it can be equipped with the raw materials such as ginger powder using ampelopsis grossdentata leaf extract as primary raw material and be prepared that mouthfeel is good, highly concentrated brown sugar ginger vine tea.Preparation section of the present invention is simply controllable, and extraction time is greatly saved and extracts raw material, entire production process is all using water as reagent, and process is simple and environmentally-friendly, green safe.
Description
Technical field
The present invention relates to a kind of ampelopsis grossdentata leaf extract and its applications in terms of health care product.
Background technique
The young stem and leaf of ampelopsis grossdentata is the alternative tea of raw material production, and therefore, ampelopsis grossdentata leaf is also known as vine tea.In China
Minority area has the draft health-care tea of long edible history, and in Hunan Zhangjiajie, vine tea is referred to as " Tujia's mind tea ",
Bama of Guangxi Yao nationality residential area, vine tea are known as " life-prolonging tea ", and flavones (based on dihydromyricetin) rich in, vine tea are more
The component contents such as sugar, vine tea polyphenol are related.The vine tea also protein containing needed by human body, amino acid, dietary fiber, carrot
The microelements such as plain, vitamin and iron, zinc, calcium, magnesium, potassium, sodium.
Vine tea processing method in the prior art is similar to green tea, in spring and early summer picked by hand tender shoots or tender leaf, warp
Airing withers, rubs, fried green or sundrying, drying form.Bloom is played on vine tea surface, and millet paste is limpid bright, and flavour is thick and solid, after first bitter
It is sweet, it promotes the production of body fluid to quench thirst.
Since existing dry vine tea is frangible, brewing time is longer, by brewing in vine tea beneficiating ingredient such as dihydromyricetin, more
The leaching rates such as sugar are very low, precipitate more influence mouthfeel, and the vine tea dried stores easily mouldy, limited shelf life for a long time, and stores up
The loss of vine tea color is easily led to during depositing, meanwhile, unsightly, endoplasm flavour is rough for vine tea shape, and fragrance has green gas, strange
Highly seasoned, soup look is yellow, and many consumers are uncomfortable, and the popularization for greatly limiting vine tea product is drunk.Therefore in the prior art with rattan
Tea is that raw material passes through solvent extraction wherein effective component, or the individually dihydromyricetin in extraction vine tea, vine tea polysaccharide, vine tea
The independent extraction of any one of polyphenol ingredient prepares vine tea, but above method constituents extraction rate is low, recovery rate only 16%
Left and right, energy consumption is big, and the recycling of solvent will also result in the increase of cost, certainly will influence the warp of comprehensive development and utilization vine tea resource
Ji benefit.
Summary of the invention
The technical problem to be solved by the present invention is to provide for the deficiencies such as ampelopsis grossdentata leaf recovery rate in the prior art is low
A kind of ampelopsis grossdentata leaf extract, effectively improves recovery rate.
Another technical problems to be solved of the present invention are to provide the application of the ampelopsis grossdentata leaf extract, provide one kind
Delicate mouthfeel, sweetness, thick taste, without tea grounds, the high new health care product of nutritional ingredient, active constituent content.
The purpose of the present invention is achieved by the following technical programs:
A kind of ampelopsis grossdentata leaf extract is provided, is to collect filtrate using microwave digestion ampelopsis grossdentata leaf and be concentrated to give
Concentrate will collect extract liquor through supercritical carbon dioxide extracting after filtered filter residue and drying, merge concentrate and extract liquor
Obtain the ampelopsis grossdentata leaf extract.
Ampelopsis grossdentata leaf (vine tea) extract contains dihydromyricetin.Chromocor compound such as dihydromyricetin can have
Effect adjusts blood lipid, blood pressure, significant to softening vessel effect, and is adjustable reabsorption, improves sleep, urine urgency-frequency,
In addition can also antibacterial anti-inflammatory, it is clearing heat and detoxicating, analgesia detumescence, throat soothing cough-relieving, therefore to the infection of the upper respiratory tract, acute and chronic pharyngitis,
Bronchitis, weakness due to chronic disease easy catching a cold and other effects are particularly significant.
Further, the extracting pressure of the supercritical carbon dioxide extracting is 28~40MPa, and extraction temperature is 65~90
DEG C, extraction time is 100~120min, and carbon dioxide flow is 22~36kg/h；Microwave Extraction power in the microwave digestion
800~1200w, 10~30min of microwave extraction time.
Preferably, the supercritical carbon dioxide extracting pressure is 30~36MPa, and extraction temperature is 72~80 DEG C, extraction
Time is 110min, and carbon dioxide flow is 26~30kg/h；Preferably, Microwave Extraction power 900 described in step S2~
1100w, microwave extraction time 15min.
Present invention simultaneously provides the application of the ampelopsis grossdentata leaf extract, using preparing health care product.
Preferably, the health care product is brown sugar ginger vine tea, including following parts by weight component: ampelopsis grossdentata leaf extract
50~80 parts, 100~320 parts of brown sugar, 15~40 parts of ginger powder, 5~25 parts of chrysanthemum powder, 3~20 parts of xylo-oligosaccharide.
Preferably, the brown sugar ginger vine tea of the vine tea powder preparation includes following parts by weight component: ampelopsis grossdentata leaf mentions
Take 55~70 parts of object, 150~300 parts of brown sugar, 18~30 parts of ginger powder, 10~20 parts of chrysanthemum powder, 7~18 parts of xylo-oligosaccharide.
Present invention simultaneously provides the preparation methods of brown sugar ginger vine tea, comprising the following steps:
S1. feedstock processing: ampelopsis grossdentata base of leaf leaf is dry, crushes, sieving, obtains vine tea powder；
S2. microwave digestion: the vine tea powder that step S1 is obtained is added into water, heating is impregnated, and filter is obtained by filtration in Microwave Extraction while hot
Liquid and filter residue；
S3. it is concentrated: after the filtrate cooling in step S2, standing, remove supernatant, stay crystal；
S4. it supercritical extract: will be passed through in supercritical carbon dioxide extracting device after filter residue and drying obtained by step S2, setting extraction
Device operating pressure and operating temperature are taken, is extracted, decompression separation merges crystal obtained by step S3, can obtain ampelopsis grossdentata
Leaf extract；
S5. mixing: by ampelopsis grossdentata leaf extract obtained by step S4, adding water, heats, and brown sugar, ginger powder, chrysanthemum is added
Powder, xylo-oligosaccharide, high-speed stirred filter to get mixed liquor；
S6. prepare brown sugar ginger vine tea: by mixed liquor high-temperature sterilization obtained by step S5, spray drying is sieved to get brown sugar ginger
Vine tea.
Further, ampelopsis grossdentata leaf content is greater than 90% in the ampelopsis grossdentata leaf extract.
Further, water described in step S1~S5 is in drinking water, purified water, water for injection or sterilized water for injection
It is a kind of.
Further, sieving mesh size described in step S1 is 20~60 mesh, and vine tea powder moisture content is 3~8%.
Preferably, sieving mesh size described in step S1 is 40 mesh, and the vine tea powder moisture content is 5%.Vine tea powder diameter
Trail can increase the contact area with water or carbon dioxide, improve extraction yield, but the partial size for working as vine tea powder is too small, is easy to stifled
Plug pipe and filter opening, make the CO by extraction column2It reduces to reducing extraction yield, and partial size is meticulous is easily brought into knockout drum,
Difficulty is brought to mask work.
Further, vine tea powder described in step S2 and water quality ratio are 1:10~25, heat soaking temperature 80~100
DEG C, 15~30min of soaking time.
Preferably, vine tea powder described in step S2 and water quality ratio are 1:20, heat 90 DEG C of soaking temperature, soaking time
20min。
Heating can promote the solubility of dihydromyricetin, accelerates intermolecular movement, can effectively improve effective component in vine tea
Recovery rate, shorten extraction time.
Microwave can be heated with penetration, greatly save extraction time, high-efficient, yield is big, while Microwave Extraction does not have
Thermal inertia, it is easily controllable.
Further, cooling temperature described in step S3 is to 0~10 DEG C, 2~3h of time of repose.
Further, heating temperature described in step S5 is 70~95 DEG C, and high-speed stirred speed is 1100~1500r/
Min, mixing time are 15~30min.
Preferably, heating temperature described in step S5 is 78~85 DEG C, and high-speed stirred speed is 1280~1420r/min,
Mixing time is 20min.
The ratio and temperature of water have larger impact to the recovery rate of dihydromyricetin, due to dihydromyricetin in the hot water molten
Xie Du is far longer than the solubility in cold water, therefore only suitable temperature cooperates water appropriate that could effectively extract dihydro
Myricetin.
Further, high-temperature sterilization temperature described in step S6 is 100~140 DEG C.
Preferably, high-temperature sterilization temperature described in step S6 is 110~128 DEG C.
Further, described in step S6 spray drying rotating speed of shower nozzle be 320~360r/min, inlet air temperature be 130~
150 DEG C of leaving air temps are 60~75 DEG C, 30~40r/min of feed flow rate；Sieving sieve described in step S6 is 20~60 mesh.
Preferably, spray drying rotating speed of shower nozzle described in step S6 is 330~340r/min, and inlet air temperature is 136~142
DEG C leaving air temp is 65~72 DEG C, feed flow rate 36r/min；Sieving sieve described in step S6 is 30~45 mesh.
The present invention extracting pressure range suitable by setting, matches optimal extraction temperature, by Huang remaining in filter residue
Ketone compound is further extracted, and extracts the effective component in vine tea to greatest extent, flavones chemical combination in vine tea is greatly improved
Object-dihydromyricetin recovery rate.
Brown sugar ginger vine tea of the present invention with xylo-oligosaccharide replace white sugar, can balance intestinal bacterium colony, attain select western Hunan vine tea, Hangzhoupro respectively
Ampelopsis grossdentata leaf powder, chrysanthemum powder and ginger powder prepared by feverfew, 27 degree of north latitude small yellow ginger is as ginger tea raw material.27 degree of little Huang of north latitude
Ginger, medical is good, and section gilvous, acrid flavour is peppery dense, and meat is delicate, and taste is fragrant, and fiber is relatively thin, ginger oil is abundant.Vine tea its contained flavones,
Dihydromyricetin and 17 kinds of amino acid and 14 kinds of needed by human body microelements, there is clearing heat and detoxicating, bactericidal antiphlogistic, analgesia to reduce swelling,
Blood fat-reducing blood pressure-decreasing, prevention cardiovascular and cerebrovascular disease, the multiple efficacies such as improve the immunity of the human body.FLOS CHRYSANTHEMI ALBA from Haizhou of China is best one product in chrysanthemum
Planting it has the function of dispelling wind and heat from the body, calming the liver, improving eyesight, clearing away heat and toxic materials, decompression, lipid-loweringing.
The invention has the following advantages:
The present invention is directed to ampelopsis grossdentata leaf extract, creative to be extracted by microwave digestion combination supercritical carbon dioxide
Dual extracting method is taken, the recovery rate of wherein active constituent dihydromyricetin is greatly improved, meanwhile, it concentrates in vine tea
Effective component, active constituent content is high in obtained brown sugar ginger vine tea, in good taste.
Further, the present invention uses microwave treatment ampelopsis grossdentata leaf, utilizes scientific specific high-frequency quick shake
It is dynamic, make intermolecular mutual collision, extruding in vine tea, conducive to the abundant leaching of effective component dihydromyricetin, to improve two
The recovery rate of hydrogen myricetin, and operation of the present invention is easy, and by-product is few.
In addition, due in ampelopsis grossdentata leaf dihydromyricetin recovery rate it is low, the present invention pass through scientific design overcritical two
Carbonoxide extraction process parameter, again extracts vine tea filter residue, further extracts dihydromyricetin.Carbon dioxide is normal
Lower temperature is gas, any chemical change does not occur in production process, product is without miscellaneous peculiar smell, to product noresidue, is easily isolated, and has
Effect has ensured the stability of heat-sensitive ingredients extraction process, and the pure natural feature of plant material has been fully retained；And titanium dioxide
Carbon has very strong permeability and solvability, and rate of extraction is fast, and extraction degree is high, and therefore, active constituent contains in extracted products
Amount is high.Carbon dioxide under condition of high voltage has bactericidal effect and antioxidation.Supercritical carbon dioxide extracting is totally-enclosed
Dark field in carry out, prevent the photic decomposition of photosensitive composition.Spray drying technology effectively prevent the viscous wall in spray-drying process existing
As reducing supplementary product consumption, effectively reducing ginger fragrance and active constituent in spray drying and scatter and disappear, obtain powder rate height, and flow of powder
Property, laxity and rehydration are good.
The present invention is directed to female menstrual period special circumstances, by the ampelopsis grossdentata leaf extract, adds ginger powder and reaches yin-yang
Coordinate, guarantees body health.Another addition modern biotechnology preparation xylo-oligosaccharide, adjusts intestinal flora balance, inhales the digestion of enteron aisle
It receives function and is in normal condition, to guarantee the absorption of nutriment, provide nutrition abundant for body.It is aobvious using the present invention
The brown sugar ginger vine tea of tooth porcelain ampelopsis leaf extract has liver protecting, auxiliary reducing blood lipid, blood pressure lowering, adjusts cardiovascular function, is anti-
Oxidation improves the effects of immunity, and gained ampelopsis grossdentata leaf chance water speed is molten, and deposit-free, stability is good, effective component
Content is high, remains the original mouthfeel of vine tea beneficiating ingredient and smell, reduces the bitterness sense of vine tea original flavor, thick taste.
The present invention is suitable for large-scale industrial production, and preparation section is simply controllable, extraction time is greatly saved, with extraction
Raw material, entire production process are all using water as reagent, and process is simple and environmentally-friendly, green safe.
Specific embodiment
It is explained further and illustrates below with reference to embodiment, but specific embodiment does not have any type of limit to the present invention
It is fixed.Unless otherwise specified, method and apparatus used in embodiment is conventional method in that art and equipment, raw materials used is normal
Advise marketable material.To simplify description, the whole preparation of typical case, that is, brown sugar ginger vine tea of present invention combination ampelopsis grossdentata leaf
Ampelopsis grossdentata leaf extract and preparation method thereof is described in detail.
Embodiment 1
The present embodiment, which prepares ampelopsis grossdentata leaf extract and is applied to, prepares brown sugar ginger vine tea, specifically includes following
Preparation step:
S1. feedstock processing: ampelopsis grossdentata base of leaf leaf is dry to be 3%, crushes, crosses 20 meshes to vine tea powder moisture content, is obtained
Vine tea powder；
S2. microwave digestion: the vine tea powder that step S1 is obtained is added into water, is heated to 80 DEG C of heat-insulation soaking 15min, microwave power
800W extracts 10min, and filtrate and filter residue are obtained by filtration while hot；Wherein vine tea powder and water quality ratio are 1:10；
S3. it is concentrated: after the filtrate in step S2 is cooled to 0 DEG C, standing 2h, remove supernatant, stay crystal；
S4. it supercritical extract: will be passed through in supercritical carbon dioxide extracting device after filter residue and drying obtained by step S2, setting extraction
Pressure power 28MPa, extracts 100min by 65 DEG C of extraction temperature, carbon dioxide flow 22kg/h, and decompression separation merges step S3
Gained crystal can obtain ampelopsis grossdentata leaf extract；
S5. mixing: ampelopsis grossdentata leaf extract obtained by step S4 is taken into 50 parts plus water, is heated to 75 DEG C, is added 100 parts
Brown sugar, 15 parts of ginger powders, 5 parts of chrysanthemum powders, 3 parts of xylo-oligosaccharides, the high-speed stirred 15min under the conditions of mixing speed is 1100r/min,
Filtering is to get mixed liquor；
S6. brown sugar ginger vine tea is prepared: by mixed liquor high-temperature sterilization under the conditions of 100 DEG C obtained by step S5, in rotating speed of shower nozzle
For 320r/min, inlet air temperature is that 130 DEG C of leaving air temps are 60 DEG C, is spray-dried under the conditions of feed flow rate 30r/min, and 20 mesh are crossed
Sieve is to get brown sugar ginger vine tea.
Total recovery rate of dihydromyricetin is 48.1% in gained brown sugar ginger vine tea preparation process.
Embodiment 2
The present embodiment, which prepares ampelopsis grossdentata leaf extract and is applied to, prepares brown sugar ginger vine tea, specifically includes following
Preparation step:
S1. feedstock processing: ampelopsis grossdentata base of leaf leaf is dry to be 4%, crushes, crosses 24 meshes to vine tea powder moisture content, is obtained
Vine tea powder；
S2. microwave digestion: the vine tea powder that step S1 is obtained is added into water, is heated to 85 DEG C of heat-insulation soaking 18min, microwave power
850W extracts 12min, and filtrate and filter residue are obtained by filtration while hot；Wherein vine tea powder and water quality ratio are 1:12；
S3. it is concentrated: after the filtrate in step S2 is cooled to 2 DEG C, standing 2h, remove supernatant, stay crystal；
S4. it supercritical extract: will be passed through in supercritical carbon dioxide extracting device after filter residue and drying obtained by step S2, setting extraction
Pressure power 30MPa, extracts 100min by 72 DEG C of extraction temperature, carbon dioxide flow 26kg/h, and decompression separation merges step S3
Gained crystal can obtain ampelopsis grossdentata leaf extract；
S5. mixing: ampelopsis grossdentata leaf extract obtained by step S4 is taken 55 parts, adds water, is heated to 75 DEG C, is added 150
Part brown sugar, 18 parts of ginger powders, 10 parts of chrysanthemum powders, 7 parts of xylo-oligosaccharides, the high-speed stirred under the conditions of mixing speed is 1200r/min
18min filters to get mixed liquor；
S6. brown sugar ginger vine tea is prepared: by mixed liquor high-temperature sterilization under the conditions of 110 DEG C obtained by step S5, in rotating speed of shower nozzle
For 330r/min, inlet air temperature is that 136 DEG C of leaving air temps are 65 DEG C, is spray-dried under the conditions of feed flow rate 34r/min, and 30 mesh are crossed
Sieve is to get brown sugar ginger vine tea.
Total recovery rate of dihydromyricetin is 48.6% in gained brown sugar ginger vine tea preparation process.
Embodiment 3
The present embodiment, which prepares ampelopsis grossdentata leaf extract and is applied to, prepares brown sugar ginger vine tea, specifically includes following
Preparation step:
S1. feedstock processing: ampelopsis grossdentata base of leaf leaf is dry to be 5%, crushes, crosses 30 meshes to vine tea powder moisture content, is obtained
Vine tea powder；
S2. microwave digestion: the vine tea powder that step S1 is obtained is added into water, is heated to 90 DEG C of heat-insulation soaking 20min, microwave power
900W extracts 15min, and filtrate and filter residue are obtained by filtration while hot；Wherein vine tea powder and water quality ratio are 1:15；
S3. it is concentrated: after the filtrate in step S2 is cooled to 4 DEG C, standing 2.5h, remove supernatant, stay crystal；
S4. it supercritical extract: will be passed through in supercritical carbon dioxide extracting device after filter residue and drying obtained by step S2, setting extraction
Pressure power 33MPa, extracts 105min by 78 DEG C of extraction temperature, carbon dioxide flow 28kg/h, and decompression separation merges step S3
Gained crystal can obtain ampelopsis grossdentata leaf extract；
S5. mixing: ampelopsis grossdentata leaf extract obtained by step S4 is taken 60 parts, adds water, is heated to 78, is added 200 parts
Brown sugar, 25 parts of ginger powders, 16 parts of chrysanthemum powders, 12 parts of xylo-oligosaccharides, the high-speed stirred under the conditions of mixing speed is 1280r/min
20min filters to get mixed liquor；
S6. brown sugar ginger vine tea is prepared: by mixed liquor high-temperature sterilization under the conditions of 110 DEG C obtained by step S5, in rotating speed of shower nozzle
For 330r/min, inlet air temperature is that 138 DEG C of leaving air temps are 70 DEG C, is spray-dried under the conditions of feed flow rate 36r/min, and 34 mesh are crossed
Sieve is to get brown sugar ginger vine tea.
Total recovery rate of dihydromyricetin is 49.4% in gained brown sugar ginger vine tea preparation process.
Embodiment 4
The present embodiment, which prepares ampelopsis grossdentata leaf extract and is applied to, prepares brown sugar ginger vine tea, specifically includes following
Preparation step:
S1. feedstock processing: ampelopsis grossdentata base of leaf leaf is dry to be 5%, crushes, crosses 40 meshes to vine tea powder moisture content, is obtained
Vine tea powder；
S2. microwave digestion: the vine tea powder that step S1 is obtained is added into a part water, is heated to 90 DEG C of heat-insulation soaking 20min, microwave function
Rate 1000W extracts 15min, and filtrate and filter residue are obtained by filtration while hot；Wherein vine tea powder and water quality ratio are 1:20；
S3. it is concentrated: after the filtrate in step S2 is cooled to 5 DEG C, standing 2.5h, remove supernatant, stay crystal；
S4. it supercritical extract: will be passed through in supercritical carbon dioxide extracting device after filter residue and drying obtained by step S2, setting extraction
Pressure power 36MPa, extracts 110min by 80 DEG C of extraction temperature, carbon dioxide flow 30kg/h, and decompression separation merges step S3
Gained crystal can obtain ampelopsis grossdentata leaf extract；
S5. mixing: ampelopsis grossdentata leaf extract obtained by step S4 is taken 65 parts, adds water, is heated to 82 DEG C, is added 270
Part brown sugar, 28 parts of ginger powders, 18 parts of chrysanthemum powders, 16 parts of xylo-oligosaccharides, the high-speed stirred under the conditions of mixing speed is 1320r/min
20min filters to get mixed liquor；
S6. brown sugar ginger vine tea is prepared: by mixed liquor high-temperature sterilization under the conditions of 120 DEG C obtained by step S5, in rotating speed of shower nozzle
For 335r/min, inlet air temperature is that 140 DEG C of leaving air temps are 70 DEG C, is spray-dried under the conditions of feed flow rate 36r/min, and 40 mesh are crossed
Sieve is to get brown sugar ginger vine tea.
Total recovery rate of dihydromyricetin is 51.7% in gained brown sugar ginger vine tea preparation process.
Embodiment 5
The present embodiment, which prepares ampelopsis grossdentata leaf extract and is applied to, prepares brown sugar ginger vine tea, specifically includes following
Preparation step:
S1. feedstock processing: ampelopsis grossdentata base of leaf leaf is dry to be 6%, crushes, crosses 50 meshes to vine tea powder moisture content, is obtained
Vine tea powder；
S2. microwave digestion: the vine tea powder that step S1 is obtained is added into water, is heated to 95 DEG C of heat-insulation soaking 25min, microwave power
1100W extracts 20min, and filtrate and filter residue are obtained by filtration while hot；Wherein vine tea powder and water quality ratio are 1:22；
S3. it is concentrated: after the filtrate in step S2 is cooled to 7 DEG C, standing 3h, remove supernatant, stay crystal；
S4. it supercritical extract: will be passed through in supercritical carbon dioxide extracting device after filter residue and drying obtained by step S2, setting extraction
Pressure power 38MPa, extracts 115min by 85 DEG C of extraction temperature, carbon dioxide flow 32kg/h, and decompression separation merges step S3
Gained crystal can obtain ampelopsis grossdentata leaf extract；
S5. mixing: ampelopsis grossdentata leaf extract obtained by step S4 is taken 70 parts, adds water, is heated to 85 DEG C, is added 300
Part brown sugar, 30 parts of ginger powders, 20 parts of chrysanthemum powders, 18 parts of xylo-oligosaccharides, the high-speed stirred under the conditions of mixing speed is 1420r/min
25min filters to get mixed liquor；
S6. brown sugar ginger vine tea is prepared: by mixed liquor high-temperature sterilization under the conditions of 130 DEG C obtained by step S5, in rotating speed of shower nozzle
For 350/min, inlet air temperature is that 145 DEG C of leaving air temps are 72 DEG C, is spray-dried under the conditions of feed flow rate 40r/min, and 55 mesh are crossed
Sieve is to get brown sugar ginger vine tea.
Total recovery rate of dihydromyricetin is 48.2% in gained brown sugar ginger vine tea preparation process.
Embodiment 6
The present embodiment, which prepares ampelopsis grossdentata leaf extract and is applied to, prepares brown sugar ginger vine tea, specifically includes following
Preparation step:
S1. feedstock processing: ampelopsis grossdentata base of leaf leaf is dry to be 8%, crushes, crosses 60 meshes to vine tea powder moisture content, is obtained
Vine tea powder；
S2. microwave digestion: the vine tea powder that step S1 is obtained is added into water, is heated to 100 DEG C of heat-insulation soaking 30min, microwave function
Rate 1200W extracts 30min, and filtrate and filter residue are obtained by filtration while hot；Wherein vine tea powder and water quality ratio are 1:25；
S3. it is concentrated: after the filtrate in step S2 is cooled to 10 DEG C, standing 3h, remove supernatant, stay crystal；
S4. it supercritical extract: will be passed through in supercritical carbon dioxide extracting device after filter residue and drying obtained by step S2, setting extraction
Pressure power 40MPa, extracts 120min by 90 DEG C of extraction temperature, carbon dioxide flow 36kg/h, and decompression separation merges step S3
Gained crystal can obtain ampelopsis grossdentata leaf extract；
S5. mixing: ampelopsis grossdentata leaf extract obtained by step S4 is taken 80 parts, adds water, is heated to 95 DEG C, is added 320
Part brown sugar, 40 parts of ginger powders, 25 parts of chrysanthemum powders, 20 parts of xylo-oligosaccharides, the high-speed stirred under the conditions of mixing speed is 1500r/min
30min filters to get mixed liquor；
S6. brown sugar ginger vine tea is prepared: by mixed liquor high-temperature sterilization under the conditions of 140 DEG C obtained by step S5, in rotating speed of shower nozzle
For 360r/min, inlet air temperature is that 150 DEG C of leaving air temps are 75 DEG C, is spray-dried under the conditions of feed flow rate 40r/min, and 60 mesh are crossed
Sieve is to get vine tea powder.
Total recovery rate of dihydromyricetin is 46.9% in gained brown sugar ginger vine tea preparation process.
Comparative example 1
Other steps of the preparation of product are same as Example 4, the difference is that step S4, the filter that step S2 is obtained
Slag adds 20 times of water, is heated to 90 DEG C of heat-insulation soaking 20min, and microwave power 1000W extracts 15min, filtrate is obtained by filtration while hot
And filter residue.Total recovery rate of dihydromyricetin is 23.2% in gained brown sugar ginger vine tea preparation process.
Comparative example 2
Other steps of the preparation of product are same as Example 4, the difference is that, microwave digestion is not used, at raw material
It is sent directly into supercritical carbon dioxide extracting device and extracts after reason.Dihydromyricetin is total in gained brown sugar ginger vine tea preparation process
Recovery rate is 21.9%.
Comparative example 3
The preparation method of brown sugar ginger vine tea is same as Example 4, the difference is that, extractor pressure is in step S4
20MPa, 72 DEG C of extraction temperature, extraction time 110min, carbon dioxide flow 30kg/h.Gained brown sugar ginger vine tea preparation process
Total recovery rate of middle ampelopsis grossdentata leaf is 31.4%.
Comparative example 4
Other steps of the preparation of product are same as Example 4, the difference is that, extractor pressure is in step S4
55MPa.Total recovery rate of dihydromyricetin is 27.6% in gained brown sugar ginger vine tea preparation process.
Comparative example 5
Other steps of the preparation of product are same as Example 4, the difference is that, 60 DEG C of extraction temperature in step S4.
Total recovery rate of dihydromyricetin is 31.9% in gained brown sugar ginger vine tea preparation process.
Comparative example 6
Other steps of the preparation of product are same as Example 4, the difference is that, the heating temperature in step S5 is 65
℃.Total recovery rate of dihydromyricetin is 38.6% in gained brown sugar ginger vine tea preparation process.
Comparative example 7
Other steps of the preparation of product are same as Example 4, the difference is that, ginger powder is not added in step S5.
Comparative example 8
Other steps of the preparation of product are same as Example 4, the difference is that, xylo-oligosaccharide is not added in step S5
Powder.
Experimental example 1: Detection of Stability
Pass through the stability of accelerated aging tests testing product.Method particularly includes: each embodiment is provided by operating instruction
Brown sugar ginger vine tea sealed package, be put into cell incubator, setting temperature be 55 DEG C, relative humidity 55%, store 60 days；
Sampled respectively at 0 day, 30 days, 60 days, detect the appearance of instant tea respectively and reconstituted using hot water (hot water is added with 0.1g/ml,
Stir 10s)；Testing result is shown in Table 1.
Table 1
By 1 experimental result of table it is found that the vine tea powder properties of product that are prepared of the present invention are more preferable compared to comparative example stability,
And obtained product, uniformly without particle, without agglomeration, dissolution is fast, and free from admixture precipitates, hence it is evident that is better than comparative example.
Experimental example 2: taste assessment
100 volunteers of instant tea are often drunk in random selection, respectively from color, form, flavour and smell, brew
4 aspects score to brown sugar ginger vine tea obtained by each embodiment, and 100 volunteers make even respectively, and carry out to every total score
It sums it up (total score full marks 100 divide).Standards of grading are as shown in table 2.
The sense organ evaluation result for the brown sugar ginger vine tea that Examples 1 to 6 and comparative example 1~8 provide is as shown in table 3.
Table 2
Table 3
By table 3 the experimental results showed that the present invention brown sugar ginger rattan tea color gloss, the particle that are prepared are uniform, sweet tea is peppery suitable
Preferably, faint scent free from extraneous odour brews rear limpid no precipitating, and pure in mouth feel is fine and smooth, pure and fresh fragrant and sweet, no suspension precipitating or impurity, form,
Flavour, brew scoring are apparently higher than comparative example.
Embodiment 3: composition detection
Brown sugar ginger vine tea sample is prepared according to the method for Examples 1 to 6 and comparative example 1~8 respectively, and its sample is carried out
Composition detection, the results are shown in Table 4:
Table 4
Note: other nutritional ingredients include protein, carrotene, vitamin, dietary fiber and microelement.
By table 4, the experimental results showed that, active constituent content is high in the vine tea powder of the method for the present invention preparation, leads in preparation process
It crosses Microwave Extraction and supercritical carbon dioxide extracting extracts vine tea effective component jointly, obtained ampelopsis grossdentata leaf recovery rate
Height, effective component retain completely, and impure amount is few, and the vine tea powder purity prepared is high, full of nutrition.Work is prepared according to the present invention
The extraction of effective component in skill parameter preparation process, ampelopsis grossdentata leaf collecting amount are apparently higher than comparative example.
Experimental example 4: sample bacterium Indexs measure
According to vine tea powder sample prepared by the embodiment of the present invention 4, bacterial regrowth detection is carried out to vine tea powder sample, as a result such as
Shown in table 5:
Table 5
| Project | Standard regulation | Content |
| Total plate count, cfu/g | N=5, c=2, m=1000, M=50000 | < 10 |
| Coliform, cfu/g | N=5, c=2, m=10, M=100 | It is not detected |
| Mould, cfu/g | ≤50 | < 5 |
| Staphylococcus aureus, cfu/g | N=5, c=1, m=100, M=1000 | It is not detected |
| Salmonella, cfu/g | N=5, c=0, m=0 | It is not detected |
As table 5 the experimental results showed that vine tea powder prepared by the present invention meets microorganism detection index.
The above description is only a preferred embodiment of the present invention, above used general explanation, specific embodiment and
Test, the present invention is described in detail, but on the basis of the present invention, can make some modifications or improvements to it, this is to this
It is obvious for the technical staff of field.Therefore, these done without departing from theon the basis of the spirit of the present invention equally replace
It changes, modify or improve, fall within the scope of the claimed invention.
Claims (10)
1. a kind of ampelopsis grossdentata leaf extract, which is characterized in that be that it is dense to collect filtrate using microwave digestion ampelopsis grossdentata leaf
Contract to obtain concentrate, will collect extract liquor through supercritical carbon dioxide extracting after filtered filter residue and drying, merges concentrate and extraction
Liquid is taken to obtain the ampelopsis grossdentata leaf extract.
2. ampelopsis grossdentata leaf extract according to claim 1, which is characterized in that Microwave Extraction function in the microwave digestion
800 ~ 1200w of rate, 10 ~ 30min of microwave extraction time.
3. ampelopsis grossdentata leaf extract according to claim 1, which is characterized in that the supercritical carbon dioxide extracting
Extracting pressure be 28~40MPa, extraction temperature be 65~90 DEG C, extraction time be 100 ~ 120min, carbon dioxide flow be 22 ~
36kg/h。
4. the application of any one of claims 1 to 3 ampelopsis grossdentata leaf extract, which is characterized in that application preparation health care
Product.
5. application according to claim 4, which is characterized in that the health care product is brown sugar ginger vine tea, including following weight
Number component: 50 ~ 80 parts of ampelopsis grossdentata leaf extract, 100 ~ 320 parts of brown sugar, 15 ~ 40 parts of ginger powder, 5 ~ 25 parts of chrysanthemum powder, low
3 ~ 20 parts of xylan.
6. application according to claim 5, which is characterized in that brown sugar ginger vine tea is prepared by following steps:
S1. feedstock processing: ampelopsis grossdentata base of leaf leaf is dry, crushes, sieving, obtains vine tea powder；
S2. microwave digestion: adding water for the vine tea powder that step S1 is obtained, and heating is impregnated, Microwave Extraction, be obtained by filtration while hot filtrate and
Filter residue；
S3. it is concentrated: after the filtrate cooling in step S2, standing, remove supernatant, stay crystal；
S4. supercritical extract: it will be passed through in supercritical carbon dioxide extracting device after filter residue and drying obtained by step S2, extractor is set
Operating pressure and operating temperature, are extracted, decompression separation, merge crystal obtained by step S3 to get the extraction of ampelopsis grossdentata leaf
Object；
S5. mixing: by ampelopsis grossdentata leaf extract obtained by step S4, adding water, heats, and brown sugar, ginger powder, chrysanthemum powder, low is added
Xylan, high-speed stirred filter to get mixed liquor；
S6. prepare brown sugar ginger vine tea: by mixed liquor high-temperature sterilization obtained by step S5, spray drying is sieved to get brown sugar ginger rattan
Tea.
7. application according to claim 6, which is characterized in that sieving mesh size described in step S1 is 20 ~ 60 mesh, rattan
Tea powder moisture content is 3 ~ 8%.
8. application according to claim 6, which is characterized in that the mass ratio of vine tea powder and water described in step S2 is 1:10
~ 25,80 ~ 100 DEG C of soaking temperature are heated, 15 ~ 30min of soaking time.
9. application according to claim 6, which is characterized in that cooling temperature described in step S3 is 0 ~ 10 DEG C, when standing
Between 2 ~ 3h；
In step S5 heating temperature be 70 ~ 95 DEG C, high-speed stirred speed be 1100 ~ 1500r/min, mixing time be 15 ~
30min。
10. application according to claim 6, which is characterized in that high-temperature sterilization temperature described in step S6 is 100 ~ 140
℃；
Spray drying rotating speed of shower nozzle described in step S6 is 320 ~ 360r/min, and inlet air temperature is that 130 ~ 150 DEG C of leaving air temps are
60 ~ 75 DEG C, 30 ~ 40r/min of feed flow rate；Sieving sieve described in step S6 is 20 ~ 60 mesh.
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| CN112826876A (en) * | 2021-02-02 | 2021-05-25 | 刘洪涛 | Application of ampelopsis grossedentata aqueous extract in preparation of preparation for improving liver injury |
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