CN102224912B - Preparation method and application of bitter gourd dietary fiber - Google Patents
Preparation method and application of bitter gourd dietary fiber Download PDFInfo
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- CN102224912B CN102224912B CN2011101514973A CN201110151497A CN102224912B CN 102224912 B CN102224912 B CN 102224912B CN 2011101514973 A CN2011101514973 A CN 2011101514973A CN 201110151497 A CN201110151497 A CN 201110151497A CN 102224912 B CN102224912 B CN 102224912B
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- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method of bitter gourd dietary fibers, which comprises the following steps: (1) washing fresh bitter gourd, cutting the bitter gourd into segments, adding water of which the amount is 4-10 times of the bitter gourd, and pulping; (2) processing the slurry obtained in the step (1) with a colloid mill, and homogenizing at high pressure; (3) heating the slurry to 80-100 DEG C and keeping for more than 30 minutes, and reducing the temperature to room temperature; (4) performing ultrasonic treatment on the slurry obtained in the step (3), adjusting the pH value to 6.5-7.5, and performing enzymolysis by adding protease with specific activity of 1,000-20,000 international units into each kilogram of bitter gourd; (5) after the enzymolysis, adding amylase with specific activity of 1,000-20,000 international units into each kilogram of bitter gourd; after the enzymolysis is finished, performing enzyme deactivation; and reducing temperature to room temperature; (6) filtering the enzymolysis liquid obtained in the step (5) to obtain a filtrate and filter residue; adding ethanol with the concentration of no less than 95% into the filtrate until the concentration is 60-85%; standing overnight; and centrifuging and filtering to obtain a precipitate; and (7) combining the filter residue and precipitate obtained in the step (6) to obtain the bitter gourd dietary fiber. Through the invention, the purity of the bitter gourd dietary fibers is over 95%, and the bitter gourd dietary fibers have a good effect in regulating blood sugar.
Description
Technical field
The present invention relates to preparation method and the purposes of food and products and health products additive agent technical field, particularly dietary fiber of bitter melon.
Background technology
Dietary fiber be a kind of can not be by the carbohydrate of human consumption, can resist the human small intestine digests and assimilates, and the summation of edible vegetalitas composition, carbohydrate and the similar material thereof that can partly or entirely ferment in the human body large intestine comprises polysaccharide, oligosaccharides, lignin and relevant plant material; Lacking dietary fiber in the diet, be one of direct or indirect reason of the diseases such as constipation, hemorrhoid, diverticulosis of colon, colon cancer, hypercholesterolemia, ischemic heart disease and diabetes, so dietary fiber is extremely important to keeping health.
At present fewer about the report of dietary fiber of bitter melon, and report utilizes complex enzyme among the CN1826976A: be comprised of papain and amylase, extract dietary fiber of bitter melon, the dietary fiber of bitter melon purity of the method report is more than 95%, just this dietary fiber is insoluble, does not extract for soluble dietary fiber specially.
Summary of the invention
The present invention is directed to deficiency, propose a kind of preparation method of dietary fiber of bitter melon, can obtain two kinds of deliquescent dietary fibers of difference, and purity is high.
In order to realize the foregoing invention purpose, the invention provides following technical scheme: a kind of preparation method of dietary fiber of bitter melon may further comprise the steps:
1., fresh balsam pear is cleaned segment, the water making beating of 4～10 times of amounts of adding;
2., step 1. slurries cross colloid mill, and high-pressure homogeneous;
3., the slurries after 2. step is processed are heated to 80～100 ℃ and keep more than the 30min, are down to room temperature;
4., step 3. slurries process through ultrasonic wave, adjust pH to 6.5～7.5, under 45 ± 5 ℃ of conditions, every kg balsam pear adds 0.1～2.0 million international units and carries out enzymolysis than the protease of vigor, enzymolysis time is 180min～500min;
5., 4. behind the enzymolysis, temperature control adds 0.1～2.0 million international units than the amylase of vigor at 50～100 ℃ by every kg balsam pear to step, enzymolysis time is more than the 30min; Enzymolysis carries out enzyme-deactivating after finishing, and is cooled to room temperature;
6., 5. enzymolysis liquid of filtration step, get filtrate and filter residue; Add in the filtrate 95% and more than ethanol to final concentration be 60%～85%, hold over night, centrifugal filtration gets sediment;
7., filter residue and the sediment of combining step in 6., be dietary fiber of bitter melon.
Preferably, step 4. the condition processed of ultrasonic wave be: 500W～5000W, 20min～90min.
Preferably, filter residue and sediment after 7. step merges reach above ethanol washing with 95%.
Preferably, described washing is at least twice.
Compared with prior art, the technological means such as the present invention is high-pressure homogeneous by balsam pear is carried out, ultrasonic wave processing, enzymolysis and alcohol precipitation can be removed the compositions such as bitter melon protein, and the method has following advantage:
1, simple, the safety of method, environmental protection, cost are low;
2, can fully extract the preparation dietary fiber of bitter melon;
3, water-soluble dietary fiber and insoluble dietary fiber have been extracted simultaneously;
4, the dietary fiber of bitter melon purity of the present invention's extraction is more than 95%;
5, the raw materials used source of the preparation method of dietary fiber of bitter melon of the present invention is wide, regulates the successful of blood sugar.
The invention allows for the dietary fiber of bitter melon that adopts above-mentioned preparation method to obtain.
Preferably, this dietary fiber comprises water-soluble dietary fiber and the dietary fiber of insoluble in water.
The 3rd purpose of the present invention is to provide dietary fiber of bitter melon to fall application in the hypoglycemic medicine in preparation.
The specific embodiment
Describe the present invention below in conjunction with specific embodiment, the description of this part only is exemplary and explanatory, should any restriction not arranged to protection scope of the present invention.
Embodiment 1:
Get the 1000.0g balsam pear, wash driedly, segment adds the making beating of 10L water, and slurries are crossed colloid mill No. 3 times, cross 3 sub-high pressure homogeneous.Be heated to 100 ℃ and keep 180min, be cooled to room temperature.Ultrasonic wave is processed, and treatment conditions are 5000W, 90min.NaOH solution with 10mol/L is regulated pH value to 7.5, and under 50 ℃ of conditions, the neutral proteinase that adds 2.0 million international units (than vigor) carries out enzymolysis, enzymolysis 500min.After protease hydrolyzed finished, regulating the pH value with the NaOH solution of 10mol/L was 7.5, is heated to 100 ℃, and the amylase that adds 2.0 million international units (than vigor) carries out enzymolysis, and the time is 180min.Be heated to 95 ℃ after enzymolysis finishes and keep 10min, be cooled to room temperature.Centrifugal, filtration get filtrate 4.8L and filter residue.Getting filtrate 4.8L, to add 95% ethanol to final concentration be 85%, and hold over night is centrifugal, filter, and gets precipitation.Merge filter residue and precipitation, wash 2 times with 95% ethanol, vacuum drying is pulverized, and crosses 100 mesh sieves, namely gets dietary fiber of bitter melon 74.57g.
Assay:
1. total dietary fiber content: 95.8% (pressing GB/T 5009.88 measures)
2. insoluble diedairy fiber content: 82.2% (pressing GB/T 5009.88 measures)
3. soluble dietary fibre content: 13.6% (pressing GB/T 5009.88 measures)
Embodiment 2:
Get the 1000.0g balsam pear, wash driedly, segment adds the making beating of 4L water, and slurries are crossed colloid mill No. 1 time, cross 1 sub-high pressure homogeneous.Be heated to 80 ℃ and keep 30min, be cooled to room temperature.Ultrasonic wave is processed, and treatment conditions are 500W, 20min.NaOH solution with 10mol/L is regulated pH value to 6.5, and under 40 ℃ of conditions, the protease that adds 0.1 million international units (than vigor) carries out enzymolysis, enzymolysis 180min.After neutral protease enzymolysis finished, regulating the pH value with the NaOH solution of 10mol/L was 6.5, is heated to 50 ℃, and the amylase that adds 0.1 million international units (than vigor) carries out enzymolysis, and the time is 30min.Be heated to 95 ℃ after enzymolysis finishes and keep 10min, be cooled to room temperature.Centrifugal, filtration get filtrate 2.2L and filter residue.Getting filtrate 2.2L, to add 95% ethanol to final concentration be 60%, and hold over night is centrifugal, filter, and gets precipitation.Merge filter residue and precipitation, wash 2 times with 95% ethanol, vacuum drying is pulverized, and crosses 60 mesh sieves, namely gets dietary fiber of bitter melon 64.24g.
Assay:
1. total dietary fiber content: 96.3% (pressing GB/T 5009.88 measures)
2. insoluble diedairy fiber content: 82.0% (pressing GB/T 5009.88 measures)
3. soluble dietary fibre content: 14.3% (pressing GB/T 5009.88 measures)
Embodiment 3:
Get the 1000.0g balsam pear, wash driedly, segment adds the making beating of 7L water, and slurries are crossed colloid mill No. 1 time, cross 1 sub-high pressure homogeneous.Be heated to 90 ℃ and keep 120min, be cooled to room temperature.Ultrasonic wave is processed, and treatment conditions are 3000W, 60min.KOH solution with 10mol/L is regulated pH value to 7.0, and under 45 ℃ of conditions, the protease that adds 1.0 million international units (than vigor) carries out enzymolysis, enzymolysis 240min.After neutral protease enzymolysis finished, regulating the pH value with the KOH solution of 10mol/L was 7.0, is heated to 80 ℃, and the amylase that adds 1.0 million international units (than vigor) carries out enzymolysis, and the time is 120min.Be heated to 95 ℃ after enzymolysis finishes and keep 10min, be cooled to room temperature.Centrifugal, filtration get filtrate 3.6L and filter residue.Getting filtrate 3.6L, to add 95% ethanol to final concentration be 75%, and hold over night is centrifugal, filter, and gets precipitation.Merge filter residue and precipitation, wash 2 times with 95% ethanol, vacuum drying is pulverized, and crosses 80 mesh sieves, namely gets dietary fiber of bitter melon 71.24g.
Assay:
1. total dietary fiber content: 97.1% (pressing GB/T 5009.88 measures)
2. insoluble diedairy fiber content: 82.1% (pressing GB/T 5009.88 measures)
3. soluble dietary fibre content: 15.0% (pressing GB/T 5009.88 measures)
Test example:
Dietary fiber of bitter melon is regulated the blood sugar test result
1 sample: adopt the prepared dietary fiber of bitter melon sample of the various embodiments described above;
2 experimental animals: Kunming mouse is provided body weight 18.0～22.0g by unming Medical College experimental animal center.
3 test methods and result:
It is some to get Kunming mouse, is divided at random 6 groups, such as table 1.Be respectively 3 groups of blank group, positive drug group, model group, 2 groups of 1 group of embodiment, embodiment and embodiment.
Test front fasting after 12 hours, the alloxan of tail vein injection 70mg/kg.Tail vein injection 5% glucose 0.4ml after 4 hours.Treated to survey blood glucose value when blood sugar rises to the peak period, and selected the mouse of blood glucose value＞16.6mmol/l as the administration object in the 3rd day of experiment.Each is organized gavage and gives corresponding medicine, once a day, positive drug sooner or later each once, successive administration 7 days.
The blood glucose value of respectively organizing experiment mice is surveyed in the last administration after 2 hours.
Table 1 dietary fiber of bitter melon is regulated the blood sugar effect
Annotate: compare * P＜0.05, * P＜0.01 with Normal group
Model and blank relatively have significant difference (P＜0.01), this time modeling of experiment success.There is not significant difference on each administration group and the model group comparative statistics.But can find out certain trend from top experimental data, the reduction blood sugar successful of dietary fiber of bitter melon.
It below only is preferred embodiment of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. the preparation method of a dietary fiber of bitter melon may further comprise the steps:
1., fresh balsam pear is cleaned segment, the water making beating of 4～10 times of amounts of adding;
2., step 1. slurries cross colloid mill, and high-pressure homogeneous;
3., the slurries after 2. step is processed are heated to 80～100 ℃ and keep more than the 30min, are down to room temperature;
4., step 3. slurries process through ultrasonic wave, adjust pH to 6.5～7.5, under 45 ± 5 ℃ of conditions, every kg balsam pear adds 0.1～2.0 million international units and carries out enzymolysis than the protease of vigor, enzymolysis time is 180min～500min;
5., 4. behind the enzymolysis, temperature control adds 0.1～2.0 million international units than the amylase of vigor at 50～100 ℃ by every kg balsam pear to step, enzymolysis time is more than the 30min; Enzymolysis carries out enzyme-deactivating after finishing, and is cooled to room temperature;
6., 5. enzymolysis liquid of filtration step, get filtrate and filter residue; Add in the filtrate 95% and more than ethanol to final concentration be 60%～85%, hold over night, centrifugal filtration gets sediment;
7., filter residue and the sediment of combining step in 6., be dietary fiber of bitter melon.
2. preparation method as claimed in claim 1 is characterized in that: the step 4. condition processed of ultrasonic wave is: 500W～5000W, 20min～90min.
3. preparation method as claimed in claim 1 is characterized in that: filter residue and sediment after 7. step merges, and with 95% and above ethanol washing.
4. preparation method as claimed in claim 3, it is characterized in that: described washing is at least twice.
5. prepared dietary fiber of bitter melon of preparation method as claimed in claim 1.
6. dietary fiber of bitter melon as claimed in claim 5, it is characterized in that: this dietary fiber comprises water-soluble dietary fiber and the dietary fiber of insoluble in water.
7. dietary fiber of bitter melon as claimed in claim 5 falls application in the hypoglycemic medicine in preparation.
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| CN2011101514973A CN102224912B (en) | 2011-06-07 | 2011-06-07 | Preparation method and application of bitter gourd dietary fiber |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103284152A (en) * | 2013-06-13 | 2013-09-11 | 江苏红豆杉药业有限公司 | Dietary fiber extracted from Chinese yew, extraction method and use |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103932200B (en) * | 2014-04-21 | 2015-07-15 | 湖北工业大学 | Method for preparing resistant-starch enriched water chestnut dreg dietary fiber |
| CN105820262A (en) * | 2016-03-31 | 2016-08-03 | 唐云龙 | Momordica polysaccharide extracting method |
| CN106729606A (en) * | 2017-01-03 | 2017-05-31 | 河北省农林科学院经济作物研究所 | Bitter gourd sugar-reducing extract complexing agent and preparation method thereof |
| CN108142825A (en) * | 2017-12-22 | 2018-06-12 | 广西兴业百谷米业有限公司 | A kind of dietary fiber rice of high-content and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1193688C (en) * | 2003-07-08 | 2005-03-23 | 杜越新 | Food for regulating blood fat and blood sugar with haw powder and balsam pear powder as material |
| CN1826976A (en) * | 2006-04-07 | 2006-09-06 | 王美岭 | Extraction of dietary fiber of bitter melon and method for preparing health-caring food by utilizing dietary fiber of bitter melon |
| CN101366806B (en) * | 2008-07-24 | 2011-06-08 | 吴茂玉 | Method of preparing balsam pear freeze-dried powder |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103284152A (en) * | 2013-06-13 | 2013-09-11 | 江苏红豆杉药业有限公司 | Dietary fiber extracted from Chinese yew, extraction method and use |
| CN103284152B (en) * | 2013-06-13 | 2015-06-10 | 江苏红豆杉药业有限公司 | Dietary fiber extracted from Chinese yew, extraction method and use |
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