CN105613940A

CN105613940A - Method for extracting Chinese chestnut peptide

Info

Publication number: CN105613940A
Application number: CN201510491138.0A
Authority: CN
Inventors: 张恒
Original assignee: Guotai Biological Technology (beijing) Co Ltd
Current assignee: Guotai Biological Technology (beijing) Co Ltd
Priority date: 2015-08-12
Filing date: 2015-08-12
Publication date: 2016-06-01

Abstract

The invention belongs to the field of bioengineering and particularly relates to a method for extracting Chinese chestnut peptide by using a biological enzymolysis technique. The method comprises the steps of raw material processing, cooking, soup extracting, hydrolyzing, filtering, concentrating, spray drying and the like. The extracting method disclosed by the invention is simple in technical process and can be continuously performed. The Chinese chestnut peptide extracted by adopting the method is high in purity and can be rapidly dissolved in water without stirring. The obtained solution is clear and transparent and stable in property, and no impurity or precipitate is separated out; and therefore, the method is suitable for industrial production.

Description

A kind of extracting method of Chinese chestnut peptide

Technical field

The invention belongs to bioengineering field, be specifically related to a kind of method using biological enzymolysis technology to extract Chinese chestnut peptide.

Background technology

Semen Castaneae is perennial deciduous fruit tree arbor, is again great Li, chief's chestnut. Semen Castaneae have the good reputation of " kings of thousand fruits ", and Semen Castaneae is sweet in flavor and cold in property, strengthening spleen, tonifying kidney, is the first-class fruit of life lengthening. Fruits in Chinese Chestnut is nutritious, and mineral is comprehensive, has potassium, zinc, ferrum etc., rich in high quality carbon hydrate, protein, vitamin and several mineral materials, high nutritive value. In every 100 grams starch-containing 51��60%, protein 5.7��10.7%, fat 2��7.4%, calcium 15 milligrams, ferrum 1.2 milligrams, 1.32 milligrams of zinc, vitamin e1 1.45 milligrams, 0.15 milligram of riboflavin, the mineral such as Catergen .5 milligram, crude fibre, carotene, vitamin A, B, C and calcium, phosphorus, potassium, it is available for the nutrient of absorption of human body and utilization up to 98%. Semen Castaneae belongs to nuts, and its content of starch is significantly high, and high starchiness contained by Semen Castaneae can provide high heat, and potassium helps maintenance normal heartbeat rule, and cellulose then can strengthen intestinal, keeps Excretory system normal operation. Auxiliary treatment is suffered from a deficiency of the kidney useful by Semen Castaneae, therefore also known as " kidney is fruit ". Semen Castaneae is the dry fruit kind that carbohydrate content is higher, the heat energy that human body is more can be supplied, and lipid metabolism can be helped, although Semen Castaneae has significantly high medical value and containing abundant nutrient substance, but owing to it contains substantial amounts of starch and saccharide, it is difficult to digest and assimilate, especially diabetes patient, overeats and affect stablizing of blood glucose. Semen Castaneae product mostly is roughing on the market at present, and the deep processing of Semen Castaneae also rests on the roughing that sugar fries " three is low " of the low value-added and low market demands of low technical content such as Semen Castaneae, Chinese chestnut sauce and Chinese chestnut can at present. These products substantially can not change the original macromolecular structure of Semen Castaneae, extremely low as the absorption in human body of a kind of macromolecular compound and utilization rate.

Along with the raising of state-of-the-art, Semen Castaneae is carried out deep processing, extract its effective ingredient Chinese chestnut peptide and be possibly realized, rich amino acids and not fatty in Chinese chestnut peptide, do not need to digest direct absorption; Biological activity by force can absorbed intact; Uncompetitive active absorption; Consuming energy low does not increase the intestines and stomach burden. Unsaturated fatty acid contained by Chinese chestnut peptide and various vitamin, have effect of resisting hypertension, coronary heart disease, osteoporosis and arteriosclerosis, be the excellent tonic product of defying age, life lengthening. Chinese chestnut peptide has the function of strengthening the spleen and stomach, QI invigorating, the kidney invigorating, strong waist, strong muscle, hemostasis and detumescence heart tonifying, it is suitable for that soreness of the waist and knees that treating suffers from a deficiency of the kidney causes, waist-leg be unfavorable, urine is increased, the chronic diarrhea caused with Deficiency and coldness of spleen and stomach, and the disease such as the fracture caused after wound, swelling and pain due to blood stasis and bones and muscles pain.

CN201310360381.X provides a kind of Chinese chestnut peptide full-nutrient wine and preparation method thereof, kernel of Semen Castaneae Mollissimae is soaked color fixative passivation tannin through citric acid solution, grind, sterilizing, addition amylase and saccharifying enzyme, addition bromelain constant temperature stand and makes protein degraded condensation as much as possible is peptide, it is eventually adding wine uses yeast fungus fermentation, extracts, obtain Chinese chestnut peptide full-nutrient wine.

CN201310360050.6 discloses a kind of castanea mollissima peptide lactic acid bacteria beverage and preparation method thereof, kernel of Semen Castaneae Mollissimae is baked to golden yellow, then add water slurrying by the weight ratio of 1: 5��7, adding the lactic acid bacteria culturers after being progressively incremented by domestication, the solidifying cake mass obtained after fermentation is castanea mollissima peptide lactic acid bacteria beverage.

Summary of the invention

The purpose of the present invention is just able to the various nutrient substance in Semen Castaneae are carried out micromolecular conversion and extraction, thus efficiently solving the problem that human body is difficult to absorb and utilize. Chinese chestnut peptide is to be made after special vegetable protein enzymatic degradation extracts by high-quality Semen Castaneae, and its molecular weight, all below 1000 dalton, has the high property digested and assimilated and utilization rate. It without gastro-intestinal digestion, directly can be absorbed by the body. Can effectively ensure human body supplementing Semen Castaneae Middle nutrition element.

In order to solve the problem existing for background technology, inventor utilizes zymolysis technique, extracts small-molecular peptides through technological processes such as raw material preliminary working, low temperature boiling, immersion, hydrolysis, concentration, dry, sprayings.

The present invention is achieved through the following technical solutions:

1, raw material processing: cleaning the kernel of Semen Castaneae Mollissimae after peeling enter making beating in beater, 230 orders filter, the Semen Castaneae powder agglomates after filtration carries out drying, drying and processing;

2, steaming and decocting: tap water is added cooker, opens stirring, intensification limit, limit puts into meter weight rice-chestnut powder, is warming up to timing insulation in 60 minutes during 65 DEG C of top, 55 DEG C, bottom, and insulation terminates, and stops stirring, stands 1.5 hours;

3, row's soup: soup storage tank and material pipe washing and sterilizing, stands and terminates to arrange soup immediately, utilize soup of vacuum extraction tank extracting, pump into a soup storage tank, and now soup temperature is 45 DEG C��55 DEG C;

4, hydrolysis: hydrolytic decomposition pot and conveyance conduit washing and sterilizing, soup is pumped into hydrolytic decomposition pot, it is warming up to 80 DEG C and keeps sterilizing in 10 minutes, it is cooled to 60 DEG C and keeps, add NaoH alkali liquor and adjust PH to neutral, putting into the hydrolysis of vegetable protein enzyme preparation, duration 1��2 hour, enzymolysis terminates the 15 minutes enzyme denaturing of 70 DEG C of insulations that heat up;

5, filter: after hydrolyzed solution enzyme denaturing, stand, utilize tub filter, two-stage plate filter to filter to clear liquid storage tank, filter complete, recycling plate filter circulating filtration 1 hour;

6, concentration: utilizing vacuum concentrator to be evaporated to concentration is 3��15 baume, concentrated solution clarification can dry by Direct spraying, if muddy, then needs carry out plate-and-frame filtration again or separate with tube centrifuge;

7, spray drying: obtain Chinese chestnut peptide fine work, inlet temperature 95��100 DEG C, leaving air temp 80��90 DEG C by spray dryer spray drying, adjust air temperature according to input concentration.

The method have the advantages that the extracting method of the present invention has technical process simple, can continuously perform that human body without any chemical composition and hormone, is not had any toxic and side effects, is very pure nutrient substance by the Chinese chestnut peptide that this method is extracted. Can being dissolved in rapidly water without stirring, aqueous solution is limpid transparent, and for white or light yellow transparent liquid, product property is stable, will not precipitate out any impurity and precipitate, be suitable to industrialized production.

Detailed description of the invention

Now further describing beneficial effects of the present invention by following example, those of ordinary skill in the art make according to the present invention simultaneously apparent change and modification are also contained within the scope of the invention.

Claims

1. an extracting method for Chinese chestnut peptide, comprises the following steps:

2, steaming and decocting: tap water adds cooker, opens stirring, intensification limit, limit puts into meter weight rice-chestnut powder, is warming up to timing insulation in 60 minutes during 65 DEG C of top, 55 DEG C, bottom, and insulation terminates, and stops stirring, stands 1.5 hours;

3, row's soup: soup storage tank and material pipe clean fire bacterium, stands and terminates to arrange soup immediately, utilize soup of vacuum extraction tank extracting, pump into a soup storage tank, and now soup temperature is 45 DEG C��55 DEG C;

4, hydrolysis: hydrolytic decomposition pot and conveyance conduit clean fire bacterium, soup is pumped into hydrolytic decomposition pot, it is warming up to 80 DEG C and keeps 10 minutes fiery bacterium, it is cooled to 60 DEG C and keeps, add NaoH alkali liquor and adjust PH to neutral, putting into the hydrolysis of vegetable protein enzyme preparation, duration 1��2 hour, enzymolysis terminates the 15 minutes fiery enzymes of 70 DEG C of insulations that heat up;

5, filter: after hydrolyzed solution enzyme denaturing, stand, utilize tub filter, two-stage plate filter to filter to clear liquid storage tank, filter complete, recycle plate filter circulating filtration;