CN106834403A - The extracting method of ganoderma lucidum polypeptide - Google Patents
The extracting method of ganoderma lucidum polypeptide Download PDFInfo
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- CN106834403A CN106834403A CN201710080423.2A CN201710080423A CN106834403A CN 106834403 A CN106834403 A CN 106834403A CN 201710080423 A CN201710080423 A CN 201710080423A CN 106834403 A CN106834403 A CN 106834403A
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- ganoderma lucidum
- lucidum polypeptide
- polypeptide
- extracting method
- enzymolysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Abstract
The invention discloses a kind of extracting method of ganoderma lucidum polypeptide.The method of the present invention by ganoderma lucidum wall-broken, is then decocted and digested again first.Method of the present invention operating method is simple, reaction condition is gentle, solve the problems, such as that ganoderma lucidum polypeptide extraction efficiency is low, extraction cost is high, ganoderma lucidum peptide masses content is low in extract, improve the extraction efficiency of ganoderma lucidum polypeptide, nutritive value obtains greatly raising, healthcare function is obviously improved, and the sound development to promoting ganoderma lucidum industry plays huge impetus.
Description
Technical field
The present invention relates to polypeptide extractive technique field, and in particular to a kind of extracting method of ganoderma lucidum polypeptide.
Background technology
Ganoderma lucidum belongs to a class fungal organism of distributed in nature, is one of traditional rare traditional Chinese medicine, dietotherapeutic.Medicine at all times
Reason proves that ganoderma lucidum truly has the effect of preventing and curing diseases, promote longevity with clinical research.Modern pharmacology research finds, in ganoderma lucidum
Contain GL-B, ganoderic acid, nucleotides, superoxide dismutase, bioflavonoid (also known as vitamin P), Panthenol, dimension
He orders C, vitamin e, protein, amino acid, trace element (calcium, potassium, zinc, iron, chromium, selenium etc.), carrotene etc., theory of traditional Chinese medical science
In, ganoderma lucidum enters the five internal organs, the gas of completion body, so the dirty weakness of the heart, lung, liver,spleen,kidney, can take it；Clinical medicine shows, ganoderma lucidum
With extensive pharmacological action, such as antitumor action, immunoregulation effect, anti-radiation and anti-Chemotherapy, sedation, cardiac stimulant
And function of resisting myocardial ischemia, regulating blood lipid action, hypoglycemic effect, antiasthmatic effect, hepatoprotective effect, oxygen lack resistant function and anti-aging
Deng effect.
At present Garnoderma product on the market can be made various products according to different purposes, and hyoscine can be made into syrup, piece
Agent, injection, electuary, vina etc., various beverages, oral liquid, tea bag etc. are can be made into for health food.Ganoderma lucidum it is effective into
Big multipotency is divided to be dissolved in hot water, wherein solubility is relatively low in the hot water for the composition such as ganoderic acid, saponin, ergosterol, and in ethanol
Solubility it is but very high, and that ethanol is extracted is costly, does not also ensure that whole reservations of effective component of glossy ganoderma；Ganoderma lucidum polypeptide
It is heated and be easily decomposed into polysaccharide and amino acid, causes to have influenceed drug effect.
Therefore comprehensive utilization and the deep development of ganoderma lucidum are carried out, the comprehensive exploitation of various active composition in ganoderma lucidum is realized, it is right
Ganoderma lucidum industry development has energetically meaning.
The content of the invention
It is an object of the invention to provide a kind of extracting method of ganoderma lucidum polypeptide, be used to solve many peptides extraction rates of ganoderma lucidum it is low,
It is difficult to mass produce, and the relatively low problem of ganoderma lucidum content of peptides in ganoderma lucidum polypeptide products.
To achieve the above object, the invention provides a kind of extracting method of ganoderma lucidum polypeptide.Specifically, the extracting method
Comprise the following steps：
First, the fragment that length, width and height are 0.8~1.2cm is cut into after ganoderma lucidum being cleaned into 2~3 times, being transferred to wall-breaking machine is carried out
Broken wall, is obtained glossy ganoderma powder；
2nd, glossy ganoderma powder is placed in interlayer retort, is warming up to 100 DEG C of top, 120 DEG C of bottom, 2 are decocted under stirring condition
~3 times, each 2h merges decoction liquor, stands 10~12h；
3rd, decoction liquor is placed in vacuum extraction tank, vacuum filtration under the conditions of 45 DEG C, extract is obtained；
4th, extract is transferred in hydrolytic decomposition pot, 100 DEG C of sterilizing 10min are cooled to rapidly 60 DEG C, add aqueous slkali to adjust
Section pH value adds enzymolysis agent enzymolysis to 7, and enzymolysis is warming up to 90 DEG C of constant temperature 15min and goes out enzyme after terminating, ganoderma lucidum polypeptide inactivator is obtained
Solution liquid；
5th, after ganoderma lucidum polypeptide inactivation enzymolysis liquid being stood into 2~3h, filtered 2~3 times using filter, abandon filter residue, Ran Houji
Continuous circulating filtration 1h；
6th, the ganoderma lucidum polypeptide inactivation enzymolysis liquid after filtering is concentrated in vacuo to concentration for 11~13 ° of B é, ganoderma lucidum polypeptide is obtained
Concentrate；
7th, ganoderma lucidum polypeptide concentrate is transferred to spray dryer, 100 DEG C of EAT, 85 DEG C of leaving air temp carries out dense
Contracting, collects dry powder and ganoderma lucidum polypeptide is obtained.
Preferably, broken wall condition is 45000~85000r/min, 4~8min in the step one.
Preferably, aqueous slkali is NaOH solution in the step 4.
Preferably, enzymolysis agent is into papain in the step 4.
Preferably, extract and the volume mass ratio of papain are 1 in the step 4：0.004-0.009.
Preferably, enzymolysis time is 4~6h in the step 4.
Preferably, filter is tub filter or two-stage plate filter in the step 5.
Preferably, the dry powder water content of gained ganoderma lucidum polypeptide is 8~10% in the step 7.
The extracting method of ganoderma lucidum polypeptide of the present invention has the following advantages that compared with prior art：
1st, extracting method of the invention, high-quality ganoderma lucidum is made after enzyme degraded extraction, and its product can be fast without stirring
Water is instantly dissolved in, is brown transparency liquid after dissolving, without any impurity and sediment, obtained ganoderma lucidum polypeptide molecular weight is 1000
Below dalton, with the high property digested and assimilated and utilization rate, can effectively ensure benefit of the human body to nutrient in ganoderma lucidum
Fill.
2nd, extracting method of the invention, many peptides extraction rates of ganoderma lucidum are high, and simple to operate, reaction condition is gentle, it is adaptable to ganoderma lucidum
Polypeptide large-scale production and application.
3rd, the ganoderma lucidum polypeptide that extracting method of the invention is extracted, without any chemical composition and hormone, does not have to human body
Any toxic and side effect, water content is 8~10%, high purity.
Specific embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Embodiment 1
The extracting method of the ganoderma lucidum polypeptide of the present embodiment, prepares according to the following steps：
Step one, by ganoderma lucidum clean 2 times after be cut into the fragment that length, width and height are 1.1cm, be transferred to wall-breaking machine 85000r/
Min broken wall 5min, are obtained glossy ganoderma powder；
Step 2, glossy ganoderma powder is placed in interlayer retort, is warming up to 100 DEG C of top, 120 DEG C of bottom, decocted under stirring condition
Boil 3 times, each 2h, merge decoction liquor, stand 12h；
Step 3, decoction liquor is placed in vacuum extraction tank, vacuum filtration under the conditions of 45 DEG C, extract is obtained；
Step 4, extract is transferred in hydrolytic decomposition pot, 100 DEG C of sterilizing 10min are cooled to rapidly 60 DEG C, add NaOH
Solution adjusts pH value to 7, adds papain enzymolysis 6h, enzymolysis that 90 DEG C of constant temperature 15min are warming up to after terminating and goes out enzyme, and spirit is obtained
Sesame polypeptide inactivates enzymolysis liquid, wherein, extract is 1 with the volume mass ratio of papain：0.006；
Step 5, by ganoderma lucidum polypeptide inactivation enzymolysis liquid stand 3h after, using tub filter or two-stage plate filter mistake
Filter 2 times, abandons filter residue, then proceedes to circulating filtration 1h；
Step 6, the ganoderma lucidum polypeptide inactivation enzymolysis liquid after filtering is concentrated in vacuo to concentration for 13 ° of B é, ganoderma lucidum polypeptide is obtained
Concentrate；
Step 7, ganoderma lucidum polypeptide concentrate is transferred to spray dryer, 100 DEG C of EAT, 85 DEG C of leaving air temp enters
It is 9% that row is concentrated into ganoderma lucidum polypeptide dry powder water content, collects dry powder, that is, ganoderma lucidum polypeptide is obtained.After measured, gained ganoderma lucidum polypeptide
Purity is 87.3%.
Embodiment 2
The extracting method of the ganoderma lucidum polypeptide of the present embodiment, prepares according to the following steps：
Step one, by ganoderma lucidum clean 2 times after be cut into the fragment that length, width and height are 1cm, be transferred to wall-breaking machine 45000r/min
Broken wall 7min, is obtained glossy ganoderma powder；
Step 2, glossy ganoderma powder is placed in interlayer retort, is warming up to 100 DEG C of top, 120 DEG C of bottom, decocted under stirring condition
Boil 3 times, each 2h, merge decoction liquor, stand 11h；
Step 3, decoction liquor is placed in vacuum extraction tank, vacuum filtration under the conditions of 45 DEG C, extract is obtained；
Step 4, extract is transferred in hydrolytic decomposition pot, 100 DEG C of sterilizing 10min are cooled to rapidly 60 DEG C, add NaOH
Solution adjusts pH value to 7, adds papain enzymolysis 5h, enzymolysis that 90 DEG C of constant temperature 15min are warming up to after terminating and goes out enzyme, and spirit is obtained
Sesame polypeptide inactivates enzymolysis liquid, wherein, extract is 1 with the volume mass ratio of papain：0.004；
Step 5, by ganoderma lucidum polypeptide inactivation enzymolysis liquid stand 3h after, using tub filter or two-stage plate filter mistake
Filter 2 times, abandons filter residue, then proceedes to circulating filtration 1h；
Step 6, the ganoderma lucidum polypeptide inactivation enzymolysis liquid after filtering is concentrated in vacuo to concentration for 12 ° of B é, ganoderma lucidum polypeptide is obtained
Concentrate；
Step 7, ganoderma lucidum polypeptide concentrate is transferred to spray dryer, 100 DEG C of EAT, 85 DEG C of leaving air temp enters
It is 8% that row is concentrated into ganoderma lucidum polypeptide dry powder water content, collects dry powder, that is, ganoderma lucidum polypeptide is obtained.After measured, gained ganoderma lucidum polypeptide
Purity is 89.7%.
Embodiment 3
The extracting method of the ganoderma lucidum polypeptide of the present embodiment, prepares according to the following steps：
Step one, by ganoderma lucidum clean 3 times after be cut into the fragment that length, width and height are 1.2cm, be transferred to wall-breaking machine 6000r/min
Broken wall 5min, is obtained glossy ganoderma powder；
Step 2, glossy ganoderma powder is placed in interlayer retort, is warming up to 100 DEG C of top, 120 DEG C of bottom, decocted under stirring condition
Boil 2 times, each 2h, merge decoction liquor, stand 10.5h；
Step 3, decoction liquor is placed in vacuum extraction tank, vacuum filtration under the conditions of 45 DEG C, extract is obtained；
Step 4, extract is transferred in hydrolytic decomposition pot, 100 DEG C of sterilizing 10min are cooled to rapidly 60 DEG C, add NaOH
Solution adjusts pH value to 7, adds papain enzymolysis 4.5h, enzymolysis that 90 DEG C of constant temperature 15min are warming up to after terminating and goes out enzyme, is obtained
Ganoderma lucidum polypeptide inactivates enzymolysis liquid, wherein, extract is 1 with the volume mass ratio of papain：0.008；
Step 5, by ganoderma lucidum polypeptide inactivation enzymolysis liquid stand 2h after, using tub filter or two-stage plate filter mistake
Filter 3 times, abandons filter residue, then proceedes to circulating filtration 1h；
Step 6, the ganoderma lucidum polypeptide inactivation enzymolysis liquid after filtering is concentrated in vacuo to concentration for 13 ° of B é, ganoderma lucidum polypeptide is obtained
Concentrate；
Step 7, ganoderma lucidum polypeptide concentrate is transferred to spray dryer, 100 DEG C of EAT, 85 DEG C of leaving air temp enters
It is 9.5% that row is concentrated into ganoderma lucidum polypeptide dry powder water content, collects dry powder, that is, ganoderma lucidum polypeptide is obtained.After measured, gained ganoderma lucidum polypeptide
Purity be 88.3%.
Embodiment 4
The extracting method of the ganoderma lucidum polypeptide of the present embodiment, prepares according to the following steps：
Step one, by ganoderma lucidum clean 2 times after be cut into the fragment that length, width and height are 0.9cm, be transferred to wall-breaking machine 75000r/
Min broken wall 8min, are obtained glossy ganoderma powder；
Step 2, glossy ganoderma powder is placed in interlayer retort, is warming up to 100 DEG C of top, 120 DEG C of bottom, decocted under stirring condition
Boil 3 times, each 2h, merge decoction liquor, stand 12h；
Step 3, decoction liquor is placed in vacuum extraction tank, vacuum filtration under the conditions of 45 DEG C, extract is obtained；
Step 4, extract is transferred in hydrolytic decomposition pot, 100 DEG C of sterilizing 10min are cooled to rapidly 60 DEG C, add NaOH
Solution adjusts pH value to 7, adds papain enzymolysis 4h, enzymolysis that 90 DEG C of constant temperature 15min are warming up to after terminating and goes out enzyme, and spirit is obtained
Sesame polypeptide inactivates enzymolysis liquid, wherein, extract is 1 with the volume mass ratio of papain：0.009；
Step 5, ganoderma lucidum polypeptide inactivation enzymolysis liquid is stood 2 after, filtered using tub filter or two-stage plate filter
3 times, filter residue is abandoned, then proceed to circulating filtration 1h；
Step 6, the ganoderma lucidum polypeptide inactivation enzymolysis liquid after filtering is concentrated in vacuo to concentration for 11 ° of B é, ganoderma lucidum is obtained many
Peptide concentrate；
Step 7, ganoderma lucidum polypeptide concentrate is transferred to spray dryer, 100 DEG C of EAT, 85 DEG C of leaving air temp enters
It is 8.5% that row is concentrated into ganoderma lucidum polypeptide dry powder water content, collects dry powder, that is, ganoderma lucidum polypeptide is obtained.After measured, gained ganoderma lucidum polypeptide
Purity be 91.9%.
Embodiment 5
The extracting method of the ganoderma lucidum polypeptide of the present embodiment, prepares according to the following steps：
Step one, by ganoderma lucidum clean 2 times after be cut into the fragment that length, width and height are 0.8cm, be transferred to wall-breaking machine 80000r/
Min broken wall 4min, are obtained glossy ganoderma powder；
Step 2, glossy ganoderma powder is placed in interlayer retort, is warming up to 100 DEG C of top, 120 DEG C of bottom, decocted under stirring condition
Boil 3 times, each 2h, merge decoction liquor, stand 10h；
Step 3, decoction liquor is placed in vacuum extraction tank, vacuum filtration under the conditions of 45 DEG C, extract is obtained；
Step 4, extract is transferred in hydrolytic decomposition pot, 100 DEG C of sterilizing 10min are cooled to rapidly 60 DEG C, add NaOH
Solution adjusts pH value to 7, adds papain enzymolysis 5.5h, enzymolysis that 90 DEG C of constant temperature 15min are warming up to after terminating and goes out enzyme, is obtained
Ganoderma lucidum polypeptide inactivates enzymolysis liquid, wherein, extract is 1 with the volume mass ratio of papain：0.0065；
Step 5, by ganoderma lucidum polypeptide inactivation enzymolysis liquid stand 3h after, using tub filter or two-stage plate filter mistake
Filter 3 times, abandons filter residue, then proceedes to circulating filtration 1h；
Step 6, the ganoderma lucidum polypeptide inactivation enzymolysis liquid after filtering is concentrated in vacuo to concentration for 11 ° of B é, ganoderma lucidum is obtained many
Peptide concentrate；
Step 7, ganoderma lucidum polypeptide concentrate is transferred to spray dryer, 100 DEG C of EAT, 85 DEG C of leaving air temp enters
It is 10% that row is concentrated into ganoderma lucidum polypeptide dry powder water content, collects dry powder, that is, ganoderma lucidum polypeptide is obtained.After measured, gained ganoderma lucidum polypeptide
Purity be 87.2%.
By above example, the method for the present invention is easy to operate, is suitable to large-scale production, and using of the invention
It is high that method extracts the ganoderma lucidum Purity for obtaining.
Ganoderma lucidum polypeptide is different from general medicine and therapeutic action is played to certain disease, also different from general nutritional health food
Only the deficiency of nutrient in a certain respect is supplemented and strengthened, but bidirectional modulation function of human body balance on the whole, transfer
Internal body vigor, adjusts human metabolism's function, improves autoimmunity ability, is promoting whole internal organ or organ function just
Normalizing.Have certain auxiliary to stomach cancer, cancer of the esophagus, lung cancer, colon cancer, carcinoma of urinary bladder, kidney, prostate cancer, oophoroma, cancer of the uterus etc.
Therapeutic effect is helped, its feature is as follows：Improve tolerance of the tumor patient to Radiotherapy chemotherapy；The leucocyte that mitigation Radiotherapy chemotherapy causes subtracts
Less, the side effect such as anorexia；Improve the cachexia of tumor patient, strengthen constitution；The immunologic function of tumor patient is improved, is increased
The antineoplastic immune power of strong body.
Although the present invention is described in detail above to have used general explanation and specific embodiment, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (8)
1. a kind of extracting method of ganoderma lucidum polypeptide, it is characterised in that the extracting method is comprised the following steps：
First, the fragment that length, width and height are 0.8~1.2cm is cut into after ganoderma lucidum being cleaned into 2~3 times, being transferred to wall-breaking machine carries out broken wall,
Glossy ganoderma powder is obtained；
2nd, glossy ganoderma powder is placed in interlayer retort, is warming up to 100 DEG C of top, 120 DEG C of bottom, 2~3 are decocted under stirring condition
Secondary, each 2h merges decoction liquor, stands 10~12h；
3rd, decoction liquor is placed in vacuum extraction tank, vacuum filtration under the conditions of 45 DEG C, extract is obtained；
4th, extract is transferred in hydrolytic decomposition pot, 100 DEG C of sterilizing 10min are cooled to rapidly 60 DEG C, add aqueous slkali regulation pH
Value adds enzymolysis agent enzymolysis to 7, and enzymolysis is warming up to 90 DEG C of constant temperature 15min and goes out enzyme after terminating, ganoderma lucidum polypeptide inactivation enzymolysis is obtained
Liquid；
5th, after ganoderma lucidum polypeptide inactivation enzymolysis liquid being stood into 2~3h, filtered 2~3 times using filter, abandon filter residue, then proceed to follow
Ring filters 1h；
6th, the ganoderma lucidum polypeptide inactivation enzymolysis liquid after filtering is concentrated in vacuo to concentration for 11~13 ° of B é, the concentration of ganoderma lucidum polypeptide is obtained
Liquid；
7th, ganoderma lucidum polypeptide concentrate is transferred to spray dryer, 100 DEG C of EAT, 85 DEG C of leaving air temp is concentrated, is received
Collection dry powder, as ganoderma lucidum polypeptide.
2. method according to claim 1, it is characterised in that broken wall condition is 45000~85000r/ in the step one
Min, 4~8min.
3. the extracting method of ganoderma lucidum polypeptide according to claim 1, it is characterised in that aqueous slkali is NaOH in the step 4
Solution.
4. the extracting method of ganoderma lucidum polypeptide according to claim 1, it is characterised in that enzymolysis agent is into wood in the step 4
Melon protease.
5. the extracting method of ganoderma lucidum polypeptide according to claim 4, it is characterised in that extract and pawpaw in the step 4
The volume mass ratio of protease is 1：0.004-0.009.
6. the extracting method of ganoderma lucidum polypeptide according to claim 1, it is characterised in that enzymolysis time is 4 in the step 4
~6h.
7. the extracting method of ganoderma lucidum polypeptide according to claim 1, it is characterised in that filter is tub in the step 5
Filter or two-stage plate filter.
8. the extracting method of ganoderma lucidum polypeptide according to claim 1, it is characterised in that gained ganoderma lucidum polypeptide in the step 7
Water content be 8~10%.
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| CN111662946A (en) * | 2020-07-17 | 2020-09-15 | 上海应用技术大学 | Method for preparing oligopeptide by enzymolysis of ganoderma lucidum water-soluble protein |
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Application publication date: 20170613 |