CN104164466A - A ginseng peptide extraction method - Google Patents
A ginseng peptide extraction method Download PDFInfo
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- CN104164466A CN104164466A CN201410302231.8A CN201410302231A CN104164466A CN 104164466 A CN104164466 A CN 104164466A CN 201410302231 A CN201410302231 A CN 201410302231A CN 104164466 A CN104164466 A CN 104164466A
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Abstract
A ginseng peptide extraction method is provided. The method includes: A) a step of cooking at a constant temperature of 120-128 DEG C under a constant pressure of 0.12-0.16 MPa for 0.5-1.5 h, discharging a ginseng extraction liquid into a soup pot and discharging the ginseng residue; B) a step of feeding the extraction liquid from the soup pot to a hydrolysis pot, heating, sterilizing, cooling to 50-56 DEG C, adding NaOH, adding 0.5% of an enzyme specially used for ginseng, and performing enzymolysis; C) a step of adding 3-5% of active carbon after the enzymolysis is finished, and performing adsorption; D) a step of performing primary filtration by a drum filter and performing protection filtration by a plate filter to a clear liquid storage tank after the active carbon in added into the enzymatic hydrolysate and the mixture is allowed to stand and layered; E) a step of concentrating, namely a step of concentrating the filtrate by a vacuum concentrating device, wherein vacuum concentration is adopted; and F) a step of heating the concentrate, maintaining the temperature to 80-90 DEG C, sterilizing, and spraying powder and drying by a spray drying tower. By the technical scheme, the beneficial effects of the method are that: the method adopts dry ginseng as a raw material and micromolecule ginseng peptide is extracted by utilization of an enzymolysis technology.
Description
Technical field
The invention belongs to Panax pharmaceutical preparation, particularly a kind ofly take dry ginseng as raw material, utilize zymolysis technique to extract the method for ginseng peptide.
Background technology
Science is in recent years thought, absorption of human body protein is mainly to absorb with the form of peptide, the molecule that peptide is comprised of amino acid, molecular weight, between 5000-200Dalton, has extremely strong activity and diversity, can use physiological function and the biological activity of peptide nutrition, regulate the physiological function of interior each system of human body and cell, swash in vivo Some Related Enzymes element, therefore, various peptides become the important substance of HUMAN HEALTH.
Peptide is the degraded product of albumen, compares with albumen, has molecular weight little, water-soluble strong, is easy to human body and the advantage such as digests and assimilates.The physiologically actives such as that protein peptide has is anti-oxidant, hypotensive, anticoagulant is active, antitumor and immunomodulatory, also have simultaneously replenish the calcium, fat-reducing, beauty and skin care and the functional performance such as delay senility.But the method for the extraction of ginseng peptide does not also have.
Summary of the invention
The invention provides a kind of extracting method of ginseng peptide, take dry ginseng as raw material, utilize zymolysis technique to extract small molecules ginseng peptide.
To achieve these goals, the invention provides following technical scheme:
An extracting method for ginseng peptide, it comprises step:
A, steaming and decocting under high pressure: adopt temperature 120-128 ℃, pressure 0.12-0.16MPa, constant temperature and pressure boiling 0.5-1.5 hour, Radix Ginseng extractive solution enters jug used for heating up water, and ginseng residue discharges;
B, enzymolysis: extracting solution is arranged into hydrolytic decomposition pot by jug used for heating up water, be cooled to 50-56 ℃ after intensification sterilizing, adds NaOH and adjust PH=7.0, add again 0.5% ginseng specific enzyme, enzymolysis 3-4 hour, stirred 1-3 minute every 20-40 minute in enzymolysis process, and enzymolysis is evenly carried out fully;
C, absorption: after enzymolysis finishes, add the gac of 3-5% to adsorb, then add after NaOH adjusts PH=6.5 to heat up to go out enzyme sterilizing and be cooled to 70-76 ℃, standing 1.5-2.5 hour;
D, filtration: enzymolysis solution adds after gac stratification, filter through bucket formula strainer initial filter and sheet frame protection, is filtered to clear liquid storage tank;
E, concentrated: filtered liquid is concentrated through vacuum concentration equipment, adopts vacuum concentration, and concentrated pressure-controlling is at-0.4MPa to-0.6MPa, and thickening temperature is controlled at 75-85 ℃, measures degree Beaume in concentration process;
F, concentrated solution heated up and remain on 80-90 ℃, carrying out sterilizing, by spray-drying tower, being dusted dry.
By implementing above technical scheme, there is following technique effect: the extracting method of ginseng peptide provided by the invention, take dry ginseng as raw material, utilize zymolysis technique to extract small molecules ginseng peptide.
Accompanying drawing explanation
Fig. 1 is the equipment flowsheet that the extracting method of ginseng peptide provided by the invention is used.
Embodiment
Technical scheme for a better understanding of the present invention, describes embodiment provided by the invention below in detail.
The embodiment of the present invention provides a kind of extracting method of ginseng peptide, it is characterized in that, comprises step:
A, steaming and decocting under high pressure: steaming and decocting under high pressure: adopt temperature 120-128 ℃, pressure 0.12-0.16MPa, constant temperature and pressure boiling 0.5-1.5 hour, Radix Ginseng extractive solution enters jug used for heating up water, and ginseng residue discharges; Dry ginseng's matter is harder, and surface is lark, section yellowish white, and form layers ring grain brown color, there be point-like resin canal and the radial crack of yellowish brown in skin zone, and gas is special, mildly bitter flavor, sweet.The advantage of fritter shape boiling: the fritter shape ginseng of doing of take is raw material, and ginseng, without smashing, is beneficial to the separated of extracting solution and slag, and operation is simple, extraction yield is high, and is conducive to the raising of product clarity.
B, enzymolysis: extracting solution is arranged into hydrolytic decomposition pot by jug used for heating up water, after intensification sterilizing, be cooled to 50-56 ℃, add NaOH and adjust PH=7.0, add again 0.5% (in dry-matter, being: ginseng specific enzyme accounts for the weight percent of ginseng dry-matter) ginseng specific enzyme (comprising: restriction endonuclease, excision enzyme and food flavor enzyme form, wherein restriction endonuclease is containing 35%, excision enzyme is containing 35%, food flavor enzyme is containing 30%), enzymolysis 3-4 hour, in enzymolysis process, every 20-40 minute, stir 1-3 minute, enzymolysis is evenly carried out fully;
C, absorption: the floss that extracting solution produces after enzymolysis can affect the clarity of product, need adsorb through gac, therefore add the gac of 3-5% (in dry-matter) to adsorb after enzymolysis finishes, then add after NaOH adjusts PH=6.5 to heat up to go out enzyme sterilizing and be cooled to 70-76 ℃, standing 1.5-2.5 hour;
D, filtration: enzymolysis solution add gac stratification normal after, through bucket formula strainer initial filter and sheet frame protection, filter, be filtered to clear liquid storage tank; Adopt the advantage of charcoal absorption: the ginseng peptide after charcoal absorption is easily accepted on sense organ,
E, concentrated: filtered liquid is concentrated through vacuum concentration equipment, adopts vacuum concentration, and concentrated pressure-controlling is at-0.4MPa to-0.6MPa, and thickening temperature is controlled at 75-85 ℃, measures degree Beaume in concentration process; Adopt the advantage of vacuum concentration: can reduce boiling point of liquid, reach at low temperatures concentrated effect, avoid the impact on Product Activity under hot conditions; Reduce the possibility that produces floss; Improve output: filtered liquid has improved drying efficiency after concentrated.
F, concentrated solution heated up and remain on 80-90 ℃, reaching sterilising effect, by spray-drying tower, being dusted dry;
G, finished product packing: adopt and pack.
The extracting method of a kind of ginseng the peptide above embodiment of the present invention being provided is described in detail, for one of ordinary skill in the art, thought according to the embodiment of the present invention, all will change in specific embodiments and applications, in sum, this description should not be construed as limitation of the present invention.
Claims (1)
1. an extracting method for ginseng peptide, is characterized in that, comprises step:
A, steaming and decocting under high pressure: adopt temperature 120-128 ℃, pressure 0.12-0.16MPa, constant temperature and pressure boiling 0.5-1.5 hour, Radix Ginseng extractive solution enters jug used for heating up water, and ginseng residue discharges;
B, enzymolysis: extracting solution is arranged into hydrolytic decomposition pot by jug used for heating up water, be cooled to 50-56 ℃ after intensification sterilizing, adds NaOH and adjust PH=7.0, add again 0.5% ginseng specific enzyme, enzymolysis 3-4 hour, stirred 1-3 minute every 20-40 minute in enzymolysis process, and enzymolysis is evenly carried out fully;
C, absorption: after enzymolysis finishes, add the gac of 3-5% to adsorb, then add after NaOH adjusts PH=6.5 to heat up to go out enzyme sterilizing and be cooled to 70-76 ℃, standing 1.5-2.5 hour;
D, filtration: enzymolysis solution adds after gac stratification, filter through bucket formula strainer initial filter and sheet frame protection, is filtered to clear liquid storage tank;
E, concentrated: filtered liquid is concentrated through vacuum concentration equipment, adopts vacuum concentration, and concentrated pressure-controlling is at-0.4MPa to-0.6MPa, and thickening temperature is controlled at 75-85 ℃, measures degree Beaume in concentration process;
F, concentrated solution heated up and remain on 80-90 ℃, carrying out sterilizing, by spray-drying tower, being dusted dry.
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Cited By (16)
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CN104561210A (en) * | 2015-01-27 | 2015-04-29 | 张恒 | Extraction method of deer bone peptide |
CN104561209A (en) * | 2015-01-27 | 2015-04-29 | 张恒 | Extraction method of black-bone chicken peptide |
CN104611398A (en) * | 2015-01-27 | 2015-05-13 | 张恒 | Oyster peptide extraction method |
CN104611399A (en) * | 2015-01-27 | 2015-05-13 | 张恒 | Method for extracting sea cucumber polypeptide |
CN105154509A (en) * | 2015-09-28 | 2015-12-16 | 宗树伟 | Extraction method of ginseng peptide |
CN105175487A (en) * | 2015-09-11 | 2015-12-23 | 北华大学 | Ginseng protein with antioxidation activity and preparation method thereof |
CN105613940A (en) * | 2015-08-12 | 2016-06-01 | 国肽生物科技(北京)有限公司 | Method for extracting Chinese chestnut peptide |
CN105669830A (en) * | 2015-12-15 | 2016-06-15 | 北京天肽生物科技有限公司 | Composite oligopeptide powder |
CN105852136A (en) * | 2016-04-07 | 2016-08-17 | 金寨金栗源生物技术有限公司 | Preparation method of ginseng peptides |
CN105886583A (en) * | 2015-01-28 | 2016-08-24 | 北京汉合盛世国际能源贸易有限公司 | Bovine bone marrow peptide extraction method |
CN105886584A (en) * | 2015-01-28 | 2016-08-24 | 张恒 | Radix puerariae peptide extraction method |
WO2016141774A1 (en) * | 2015-03-11 | 2016-09-15 | 吉林肽谷生物工程有限责任公司 | Uses of jilin ginseng oligopeptide in preparing food product or healthcare food product for improving and enhancing sexual function |
CN106072567A (en) * | 2016-07-01 | 2016-11-09 | 谢少韩 | A kind of ginseng product and preparation technology thereof |
WO2016206363A1 (en) * | 2015-06-26 | 2016-12-29 | 吉林肽谷生物工程有限责任公司 | Use of jilin ginseng oligopeptide in preparation of anti-oxidant food product or health care food product |
CN111227102A (en) * | 2020-03-05 | 2020-06-05 | 吴伟 | Preparation method and application of ginseng active peptide |
CN117925766A (en) * | 2024-03-22 | 2024-04-26 | 吉林肽谷生物工程有限责任公司 | Preparation method of ginseng peptide with protective effect on acute alcoholic liver injury |
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CN102652829A (en) * | 2012-04-25 | 2012-09-05 | 宗树伟 | Extraction method of ginseng peptide |
CN102988440A (en) * | 2012-12-07 | 2013-03-27 | 山东大学 | Method for extracting ginsenoside |
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2014
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Patent Citations (2)
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CN102652829A (en) * | 2012-04-25 | 2012-09-05 | 宗树伟 | Extraction method of ginseng peptide |
CN102988440A (en) * | 2012-12-07 | 2013-03-27 | 山东大学 | Method for extracting ginsenoside |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104561209A (en) * | 2015-01-27 | 2015-04-29 | 张恒 | Extraction method of black-bone chicken peptide |
CN104611398A (en) * | 2015-01-27 | 2015-05-13 | 张恒 | Oyster peptide extraction method |
CN104611399A (en) * | 2015-01-27 | 2015-05-13 | 张恒 | Method for extracting sea cucumber polypeptide |
CN104561210A (en) * | 2015-01-27 | 2015-04-29 | 张恒 | Extraction method of deer bone peptide |
CN105886583A (en) * | 2015-01-28 | 2016-08-24 | 北京汉合盛世国际能源贸易有限公司 | Bovine bone marrow peptide extraction method |
CN105886584A (en) * | 2015-01-28 | 2016-08-24 | 张恒 | Radix puerariae peptide extraction method |
WO2016141774A1 (en) * | 2015-03-11 | 2016-09-15 | 吉林肽谷生物工程有限责任公司 | Uses of jilin ginseng oligopeptide in preparing food product or healthcare food product for improving and enhancing sexual function |
WO2016206363A1 (en) * | 2015-06-26 | 2016-12-29 | 吉林肽谷生物工程有限责任公司 | Use of jilin ginseng oligopeptide in preparation of anti-oxidant food product or health care food product |
CN105613940A (en) * | 2015-08-12 | 2016-06-01 | 国肽生物科技(北京)有限公司 | Method for extracting Chinese chestnut peptide |
CN105175487A (en) * | 2015-09-11 | 2015-12-23 | 北华大学 | Ginseng protein with antioxidation activity and preparation method thereof |
CN105154509A (en) * | 2015-09-28 | 2015-12-16 | 宗树伟 | Extraction method of ginseng peptide |
CN105669830A (en) * | 2015-12-15 | 2016-06-15 | 北京天肽生物科技有限公司 | Composite oligopeptide powder |
CN105852136A (en) * | 2016-04-07 | 2016-08-17 | 金寨金栗源生物技术有限公司 | Preparation method of ginseng peptides |
CN106072567A (en) * | 2016-07-01 | 2016-11-09 | 谢少韩 | A kind of ginseng product and preparation technology thereof |
CN111227102A (en) * | 2020-03-05 | 2020-06-05 | 吴伟 | Preparation method and application of ginseng active peptide |
CN117925766A (en) * | 2024-03-22 | 2024-04-26 | 吉林肽谷生物工程有限责任公司 | Preparation method of ginseng peptide with protective effect on acute alcoholic liver injury |
CN117925766B (en) * | 2024-03-22 | 2024-05-17 | 吉林肽谷生物工程有限责任公司 | Preparation method of ginseng peptide with protective effect on acute alcoholic liver injury |
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