WO2016206363A1 - Use of jilin ginseng oligopeptide in preparation of anti-oxidant food product or health care food product - Google Patents

Abstract

A use of a Jilin ginseng oligopeptide in preparation of an anti-oxidant food product or a health care food product.

Description

[Name of invention established by ISA according to Rule 37.2] Use of Jilin ginseng oligopeptide in the preparation of antioxidant foods or health foods Technical field

The invention relates to a new use of Jilin ginseng oligopeptide, in particular to the use of Jilin ginseng oligopeptide in anti-oxidation function food or health food.

Background technique

Oxidative Stress refers to the excessive production of high activity molecules such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the body when subjected to various harmful stimuli. The degree of oxidation exceeds the removal of oxides, and the oxidative system and the antioxidant system are unbalanced, resulting in damage to biological cells such as tissues and proteins and nucleic acids. Studies have confirmed that oxidative stress can lead to islet β cell function damage and peripheral insulin resistance, leading to diabetes; can cause endothelial cell dysfunction, leading to cardiovascular disease; in addition, can also cause autoimmune diseases, blood system diseases And a series of diseases such as cancer, causing great damage to human health. Therefore, controlling oxidative stress plays an extremely important role in maintaining the health of the body. Finding safe and effective antioxidants is especially urgent.

At present, the main anti-oxidation drugs for clinical use include monoamine oxidase inhibitors, dopamine receptor agonists, and vitamins. These drugs have varying degrees of side effects and should not be taken for long periods of time. Numerous studies in recent years have confirmed that natural food ingredients are safe and effective in terms of antioxidants.

A bioactive peptide refers to a peptide compound that is beneficial or physiologically active to the living activities of a living organism. Peptides are intermediate in amino acid and protein in molecular structure, but they have functions that cannot be replaced by amino acids and proteins. Oligopeptides, also known as active small molecule peptides, generally consist of 10 or fewer amino acids. Studies have found that oligopeptides are more effective than single amino acid absorption and can directly participate in protein synthesis, thus improving the body's protein utilization. In the case of obstacles in the amino acid transport system, the ingestion of oligopeptides can achieve good absorption. In addition, bioactive peptides have a wide range of biological activities, such as immune regulation, cholesterol lowering, blood pressure lowering, antithrombotic, anti-cancer, anti-viral, anti-oxidation and scavenging free radicals, and promote growth. At present, bioactive peptides have emerged with their high efficiency and safety, and gradually show their important role in clinical nutrition and broad application prospects.

Ginseng is a valuable Chinese herbal medicine. The medicinal use of ginseng has been more than 4,000 years old. At present, there are four main types of ginseng in the world: China's "Jilin Ginseng", North Korea's "Gao Lishen", Japan's "American ginseng" and Canada, the United States' "American ginseng". The ginseng of our country is the dry root and rhizome of the ginseng of the Araliaceae plant, which is mainly produced in Jilin Province. On September 4, 2012, the Chinese Ministry of Health approved ginseng as a new resource food. The application of ginseng will be expanded from a single Chinese herbal medicine to food, beverage and health care products, and the scope will be greatly expanded, which provides a new development for China's ginseng industry. Opportunity. Ginseng has the effects of replenishing vitality, quenching thirst, and calming the nerves. Studies have found that ginseng can reduce the content of lipid oxidation products such as malondialdehyde (MDA) by increasing the activities of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Play an antioxidant role. Studies on the ginseng extract ginsenoside have also found that ginsenoside Re, Rg1 and the like have antioxidant functions. Although ginseng oligopeptides exist as an important class of compounds in ginseng tissues, their functional studies have not been reported, and studies on their antioxidant functions have not been reported. The ginseng oligopeptide is easily digested and absorbed in the human gastrointestinal tract, thereby avoiding waste of the active substance and greatly improving its biological activity, and can be used as a novel formula for food or health care products having an antioxidant function.

Summary of the invention

The object of the present invention is to provide a low-viscosity, easily absorbed and fast-absorbing Jilin ginseng oligopeptide in the preparation of antioxidant functional foods or health foods, and provides a new way for improving antioxidant function through diet.

The foods described therein are powders, tablets, liquid beverages, milk powder and dairy products, confectionery or baked goods.

The health food described therein is a powder, a tablet, a capsule or an oral liquid.

The invention finds that the existing substance-Jilin ginseng oligopeptide has anti-oxidation function and finds that it is preparing antioxidant activity. Use in energy or health foods. Animal experiments have found that the ginseng oligopeptide aqueous solution of Jilin ginseng can effectively improve the antioxidant function of oxidized model rats.

detailed description

The invention is further illustrated by the following examples, which are to be considered as illustrative and not restrictive.

Example 1. Experimental study on the effect of antioxidant model on oxidation function in rats

I. Materials and methods

1 sample: Jilin ginseng oligopeptide, provided by Jilin Peptide Bioengineering Co., Ltd., light yellow solid powder, the main component is a small molecule oligopeptide with molecular weight less than 1000, the content is 97.4%.

2 Experimental animals: 90 healthy adult SPF adult male SD rats, at the end of the adaptation period, weighing 200±20 g, provided by the Experimental Animal Center of Peking University Medical School. The experimental animals were kept in cages, 3 per cage, free to eat and drink. The animal breeding laboratory meets the national standard SPF level, the temperature range is 22±2°C, the relative humidity is 50%～60%, and the nighttime alternation time is 12h:12h.

3 dose grouping and test sample administration time: 6 dose groups (0.0625g/kg BW, 0.125g/kg BW, 0.25g/kg BW, 0.50g/kg BW, 1.00g/kg BW, 2.00g) /kg BW), 1 blank control group, 1 whey protein group (0.25 g/kg BW) and 1 model control group. The test sample was given for 45 days.

Second, the experimental method

1 animal modeling, grouping and giving test objects

1.1 Adaptation period: Rats were fed with basic diet for 5-7 days under the barrier system.

1.2 Modeling period

They were randomly divided into 2 groups according to body weight, 10 rats were used as blank control group, and the remaining 80 were used as model group. The blank control group was intraperitoneally injected with sterile physiological saline daily, and the injection amount was 0.2 mL/100 g once a day. The model group was intraperitoneally injected with D-galactose 125 mg/kg BW, and the injection volume was 0.2 mL/100 g (31.25 g of D-galactose was dissolved in 500 ml of physiological saline to prepare D-galactose-sodium chloride injection. Before use, routine sterilization was used), once a day, continuous modeling for 6 weeks, blood MDA was taken, grouped according to MDA level, randomly divided into 1 model control group, 1 whey protein group and 6 recipients. Test sample dose group.

1.3 Test sample is given

After grouping, the six dose groups were intragastrically administered with different concentrations of ginseng peptide, the blank control group and the model control group were given the same volume of distilled water, and the whey protein group was given the same volume of whey protein aqueous solution. At the same time as the test sample, the model control group, the whey protein group and each dose group continued to give the same dose of D-galactose intraperitoneal injection, and the blank control group continued to give the same dose of normal saline intraperitoneal injection.

During the experiment, each mouse was weighed once a week, and the amount of intraperitoneal injection and the amount of gastric perfusion were adjusted according to the body mass. The skin color, diet, drinking water, defecation, and mental activity of the rats were observed daily.

2 detection of oxidative stress indicators

After the last intragastric and intraperitoneal injection, the rats were fasted for 12 hours after the water was fasted, and the rats were killed by decapitation.

2.1 Detection of serum oxidative stress indicators

The blood was centrifuged at 3000 r/min for 10 min, and serum was taken according to the kit instructions. 8-Hydroxy-isoprostane (8-Isoprostane), malondialdehyde (MDA), protein carbonyl, superoxide dismutase (SOD), glutathione Glycopeptide peroxidase (GSH-Px), reduced glutathione (GSH) levels.

2.2 Detection of liver oxidative stress indicators

The liver was peeled off in an ice bath, the adipose tissue was removed, the tissue was rinsed with pre-cooled physiological saline until no blood color, and the same amount of tissue was weighed. The tissue block was cut with ophthalmic scissors, and the corresponding multiple pre-cooled physiological saline was added to homogenate. The mechanism is to test the required percentage (10%) of tissue homogenate (homogenization time 10s / time, interval 30s, in an ice bath), 3000r / min cryoprecipitate for 10min, take the supernatant, according to the kit instructions Determination of malondialdehyde (MDA), protein carbonyl, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and reduced glutathione (GSH) content.

Third, statistical methods

All experimental data is wrong! The reference source was not found. ±s indicates. One-way ANOVA was performed using SPSS20.0 software, p<0.05 was statistically significant.

Fourth, the experimental results

1. Effects of Jilin ginseng oligopeptide on lipid oxidation products in oxidative model rats

As shown in Table 1, at the end of the experiment, compared with the blank control group, the levels of serum 8-epoxyisoprostane, malondialdehyde and liver malondialdehyde in the model control group were increased, among which serum C The increase in dialdehyde levels was most significant (p < 0.05). Compared with the model control group, the serum 8-epoxyisoprostane level of the ginseng oligopeptide C and D dose groups was significantly decreased (p<0.05); the Jilin ginseng oligopeptide A, B, D, F doses The serum malondialdehyde level of the rats in the group was significantly decreased (p<0.05); the malondialdehyde level in the liver of the ginseng oligopeptide A, B, D and F groups was significantly decreased (p<0.05). Compared with the whey protein group, there was no significant change in serum 8-epihydroxyisoprostane levels in the rats of Jilin ginseng oligopeptide. The difference was not statistically significant (p>0.05). Jilin ginseng oligopeptide A The levels of serum malondialdehyde in rats in groups B, D and F were significantly lower (p<0.05). The levels of malondialdehyde in the liver of Jilin ginseng oligopeptide F group were significantly lower (p<0.05).

Table 1 Effect of ginseng oligopeptide on lipid oxidation products in oxidized model rats

n=10)

Note: a: The difference was significant compared with the blank control group (p<0.05);

b: There was significant difference compared with the model control group (p<0.05);

c: The difference was significant compared with the whey protein group (p<0.05).

2. Effect of Jilin ginseng oligopeptide on protein oxidation products in oxidative model rats

As shown in Table 2, at the end of the experiment, compared with the blank control group, the serum protein carbonyl level of the model control group was significantly increased, and the difference was statistically significant (p<0.05). Compared with the model control group, serum carbonyl levels in the whey protein group and Jilin ginseng oligopeptide A, B, C, D and E dose groups were significantly lower (p<0.05); Jilin ginseng oligopeptide F dose group Rat liver protein carbonyl levels were significantly increased (p < 0.05). Compared with the whey protein group, the serum and liver protein carbonyl levels of the Jilin ginseng oligopeptide F dose group were significantly increased (p<0.05).

Table 2 Effect of ginseng oligopeptide on protein oxidation products in oxidative model rats (

n=10)

Note: a: The difference was significant compared with the blank control group (p<0.05);

b: There was significant difference compared with the model control group (p<0.05);

c: The difference was significant compared with the whey protein group (p<0.05).

3. Effect of Jilin ginseng oligopeptide on antioxidant enzyme activity in oxidative model rats

As shown in Table 3, at the end of the experiment, compared with the blank control group, the serum and liver superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels in the model control group were absent. Significant changes, the difference was not statistically significant (p>0.05). Compared with the model control group, the serum SOD level of the ginseng oligopeptide C and E dose groups was significantly increased (p<0.05); the Jilin ginseng oligopeptide B, C, D, F dose group had significant liver SOD levels. The increase was (p<0.05); the GSH-Px level in the liver of Jilin ginseng oligopeptide B, D, F dose group was significantly increased (p<0.05); compared with the whey protein group, Jilin ginseng oligopeptide E dose group The serum SOD level in rats was significantly increased (p<0.05); the serum GSH-Px levels in rats with ginseng oligopeptides A, B, C and F were significantly increased (p<0.05); Jilin ginseng oligopeptides B, C, The liver SOD levels in the D and F dose groups were significantly increased (p<0.05).

Table 3 Effect of ginseng oligopeptide on antioxidant enzyme activity in oxidized model rats (

n=10)

Note: a: The difference was significant compared with the blank control group (p<0.05);

b: There was significant difference compared with the model control group (p<0.05);

c: The difference was significant compared with the whey protein group (p<0.05).

4. Effect of Jilin ginseng oligopeptide on antioxidant protein GSH in oxidative model rats

As shown in Table 4, at the end of the experiment, there was no significant change in serum and liver reduced glutathione (GSH) levels in the model control group compared with the blank control group, and the difference was not statistically significant (p>0.05). . Compared with the model control group, the serum GSH levels of the ginseng oligopeptide B and C dose groups were significantly increased (p<0.05); the Jilin ginseng oligopeptides were dosed. There was no significant change in GSH level in rat liver, and the difference was not statistically significant (p>0.05). Compared with the whey protein group, the serum GSH level of the ginseng oligopeptide B and C dose groups was significantly increased (p<0.05); the liver GSH level of the Jilin ginseng oligopeptide C dose group was significantly increased (p<0.05). There was no significant change in the other groups, and the difference was not statistically significant (p>0.05).

Table 4 Effect of ginseng oligopeptide on antioxidant protein GSH in oxidative model rats

n=10)

Note: a: The difference was significant compared with the blank control group (p<0.05);

b: There was significant difference compared with the model control group (p<0.05);

c: The difference was significant compared with the whey protein group (p<0.05).

V. Experimental conclusion

In this study, a rat model of D-galactose oxidation was established, and a blank control group, a model control group and a whey protein group were established as a control group to investigate the effect of Jilin ginseng oligopeptide on the antioxidant activity of rats.

At the end of the experiment, serum 8-epoxyisoprostane levels, serum and liver malondialdehyde (MDA) levels, serum protein carbonyl levels, and liver superoxide disproportionation were increased in the model control group compared with the blank control group. The level of enzyme (SOD) was decreased, and the serum malondialdehyde (MDA) and protein carbonyl levels were the most significant, indicating that the experimental oxidation model was successfully established.

The results showed that Jilin ginseng oligopeptides can significantly reduce the levels of lipid oxidation products and protein oxidation products in rats, increase the activity of antioxidant enzymes in rats, and thus exert antioxidant function, and have potential as a new antioxidant functional preparation. The suitable concentration range is from 0.0625 to 1 g/kg bw per day.

Claims (3)

1. The use of Jilin ginseng oligopeptide in preparing anti-oxidation function food or health food.
2. The use of the Jilin ginseng oligopeptide according to claim 1 for preparing an antioxidant function food or health food, characterized in that the antioxidant function food is powder, tablet, liquid beverage, milk powder and dairy product , candy or baked goods.
3. The use of the Jilin ginseng oligopeptide according to claim 1 for preparing an antioxidant-resistant food or health food, characterized in that the antioxidant-resistant health food is a powder, a tablet, a capsule or an oral liquid.