JP2018070570A - Lindera plant extract - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an excellent composition for preventing and/or improving lifestyle disease that is natural, has high safety, and can be applied to food and drink.SOLUTION: A composition for preventing and/or improving lifestyle disease is prepared that contains Lauraceae Lindera plants or extract therefrom.SELECTED DRAWING: Figure 14

Description

  The present invention has excellent life-style related disease prevention and / or improvement effects such as anti-obesity activity, muscle endurance improvement activity, and liver function improvement activity, and is useful as a food, beverage, medicine, or quasi-drug. Related to natural products.

  It is important to prevent obesity so as to be a source of all illness. In recent years, research on the effects of fat on the body has progressed, and it has been suggested that visceral fat accumulation is not only obese, but also highly correlated with lifestyle-related diseases, hyperlipidemia, impaired glucose tolerance, hypertension, etc. It is believed that not accumulating fat can prevent many diseases. The pathological condition based on these ideas is metabolic syndrome. According to the Ministry of Health, Labor and Welfare, one out of every two middle-aged men has metabolic syndrome, and about 20 million people are expected to fall under the metabolic syndrome and its reserve group (Non-patent Document 1).

  In Japan, the population is aging rapidly and locomotive syndrome has become a major social problem. One factor of locomotive syndrome is a decrease in muscle mass and strength associated with aging (Non-patent Document 2). In general, high-intensity strength training is the most effective way to prevent a decrease in muscle mass and strength, but there is a concern that elderly people may be accompanied by mental stress and cardiovascular stress. Therefore, for strength training in the elderly, muscle strength training with low strength that has low mental stress and a load on the circulatory organ has been proposed. However, it is difficult for muscle strength training with low strength to easily increase muscle mass and strength. Therefore, it is important to increase endurance and perform strength training for a long time.

  The liver is a vital organ that plays a central role in metabolism. Liver functions are generally divided into circulatory function, excretory function, metabolic function, protective / detoxifying function, and hematological function. If any of these functions is impaired, fatigue, malaise, loss of appetite, gallbladder In addition, various symptoms peculiar to liver dysfunction such as slight fever appear. If the state of liver dysfunction continues, hepatitis, cirrhosis, and even lifestyle-related diseases (adult diseases) such as liver cancer may be caused (Non-patent Document 3). Therefore, maintaining normal liver function normally is extremely important for busy modern people to spend their daily life magnificently and to prevent lifestyle-related diseases.

2006 National Health and Nutrition Survey Report, Ministry of Health, Labor and Welfare (http://www.mhlw.go.jp/bunya/kenkou/eiyou08/01.html) Japan Clinical Orthopedic Association homepage (http://www.jcoa.gr.jp/locomo/) The actual condition of alcoholic liver disorders in Japan From the results of nationwide aggregation (Japanese Gastroenterology Journal Vol. 76 (1979) No. 11 P 2178-2185)

  Currently, in Japan, attention is focused on preventive medicine in order to reduce the cost of medical care, and there is a strong desire for the development of effective and safe food, beverage, medicine or quasi-drug that can reduce the risk of disease. It is rare.

  There are also nutritional supplements on the market to increase endurance, but the effects of endurance-improving ingredients such as carnitine that are generally on the market are questioned, such as locomotive syndrome. In order to improve the pathological condition, the development of a dietary supplement for improving endurance is strongly desired.

  In addition, alcoholic hepatitis, fatty liver, cirrhosis, and other liver diseases are increasing along with the increase in alcohol intake and high-calorie diet. It is of course important to solve such a serious problem by the administration of pharmaceuticals for the purpose of preventing / improving liver function. However, some liver diseases such as alcoholic hepatitis and fatty liver progress slowly and develop seriously after a long period of time. In such cases, daily care is particularly important. From the aspect of long-term administration, it is also necessary to deal with the method of taking food and drink. Under such circumstances, it is desired to develop foods and drinks that are highly effective in preventing and improving liver dysfunction.

  Therefore, an object of the present invention is to provide an excellent obesity-improving agent, muscle endurance-enhancing agent, and / or liver function-improving agent that is derived from a natural product, has high safety, and can be applied to foods and drinks. There is to do.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have found that a camphoraceae genus Cromoji or an extract or purified product thereof has an obesity improvement effect, a muscle endurance improvement effect, a liver function improvement effect, and the like. It has been found that it has excellent lifestyle-related disease prevention and / or improvement effects, and has led to the completion of the present invention.

That is, the present invention
Item 1.
A composition for preventing and / or improving lifestyle-related diseases, comprising a plant of the genus Kuromoji or an extract thereof.
Item 2.
Item 2. The composition for preventing and / or improving lifestyle-related diseases according to Item 1, wherein the lifestyle-related diseases are obesity, muscle endurance reduction or liver function decline.
Item 3.
Item 3. The lifestyle according to item 1 or 2, wherein the plant of the genus Kuromoji is derived from one or more selected from the group consisting of whole plants or leaves, stems, roots, seeds, and flowers of the plant of the genus Kuromoji. A composition for disease prevention and / or improvement.
Item 4.
Item 4. The composition for preventing and / or improving lifestyle-related diseases according to any one of Items 1 to 3, wherein the variety of the genus Kuromoji is one or more selected from the group consisting of Kermomoji, Himekuromoji, and Oobakuromoji. object.
Item 5.
Item 5. The composition for preventing and / or improving lifestyle-related diseases according to any one of Items 1 to 4, wherein the extract is a water extract, an organic solvent extract or a water-containing organic solvent extract.
Item 6.
Item 6. The composition for preventing and / or improving lifestyle-related diseases according to any one of Items 1 to 5, which is administered orally, intravenously, by inhalation, or transdermally.
Item 7.
Item 7. The composition for preventing and / or improving lifestyle-related diseases according to any one of Items 1 to 6, which is a food and drink product, a quasi drug, or a pharmaceutical product.
Item 8.
Item 8. The composition for preventing and / or improving lifestyle-related diseases according to any one of Items 1 to 7, wherein the food or drink is a tea beverage.

  The composition for preventing and / or improving lifestyle-related diseases of the present invention contains an active ingredient obtained from a naturally derived plant of the genus Chromodi, has no safety problem, obesity improvement effect, muscle endurance improvement effect, In addition, it has excellent lifestyle-related disease prevention and / or improvement effects such as liver function improvement effects. Such a safe composition for preventing and / or improving lifestyle-related diseases can be used for foods and drinks, pharmaceuticals or quasi drugs with high safety and few side effects.

It is a graph which shows the triglyceride value in mouse | mouth serum. It is a graph which shows the cholesterol level in mouse | mouth serum. It is a graph which shows the result of a lipase inhibitory activity evaluation test. It is a graph which shows the time-dependent change of the body weight of a mouse | mouth. It is a graph which shows the weight of the liver of a mouse | mouth. It is a graph which shows the weight of the vice testicle of a mouse | mouth. It is a graph which shows the weight around the kidney of a mouse | mouth. It is a graph which shows the weight of the mesentery of a mouse | mouth. It is a graph which shows the weight of a mouse | mouth brain. It is a graph which shows the weight of the visceral fat of the whole body of a mouse | mouth. It is a graph which shows the expression level of GLUT4 gene in a mouse | mouth muscle cell. It is a graph which shows the expression level of UCP-3 gene in a mouse | mouth myocyte. It is a graph which shows the glucose level in mouse | mouth serum. It is a graph which shows the GOT value in mouse | mouth serum. It is a graph which shows the GPT value in mouse | mouth serum. It is a histological image of a liver right lobe section of a mouse in an alcohol tolerance test. It is a graph which shows the GOT value in mouse | mouth serum. It is a graph which shows the GPT value in mouse | mouth serum.

  The composition for preventing and / or improving lifestyle-related diseases of the present invention contains a plant belonging to the genus Lauraceae, or an extract or purified product thereof. Here, the extract includes a purified product purified to a single component or a fraction close to a single component.

  The black genus plant is widely distributed in Japan, Southeast Asia and the like, and about 100 kinds are known. In Japan, it is common in Honshu, Shikoku and Kyushu. Chromoji, which has aromas in its leaves and roots and is a plant belonging to the genus Kuromoji, has been decocted and drunk in the Oki Islands of Shimane Prefecture.

  The black genus plant is a highly regenerative plant that grows both from seedlings (growing from seeds), from germination regeneration (growing from stumps), and from old tree roots. It can be said that it is very easy to use because it can be re-collected 2 to 3 years after cutting.

  As long as the effects of the present invention are exhibited, the varieties of the genus Kuromoji are not limited as long as they are plants contained in the genus Kuromoji, but are not limited to L. strynifolia, L. citriodora, L. communis. ), Okinawa var. Okinawensis, L. erythrocarpa, L. glaca, L. communis, L. communis (L. communis), L. communis (L. communis), L. communis (L. communis). var. lancea Mojyama), L. obtusiloba, L. obtusiloba f.v llosa) Abrachan (L. praecox), L. praecox BLUME formagustifolia (SUGIMOTO), Carebrachan (L. praecox var. pubescens), Kecrosage (L. ), Shiromoji (L. triloba), Mareboshi moji (Parabenzoin trilobum f. Integrum), Keboshi momoji (Parabenzoin trilobum f. Pirasum), Kuroboshi (L. umbelata), Kimino Plane L. umbellata var. Aurantica, L. akoensis, L. fragrans, L. fruticosa, L. acarisis, L. megaphylla, L. metcalfiana, L. metcalfiana, L. metcalfiana, L. Pratiti, L. pulcherrima var. Attenua, L. pulcherrima var. Hemsleyana, L. reflexa, L. setchuensis, L. Thomsonii, L.M. tonkiensis, L.M. tonkiensis var. Subsessilis etc. are mentioned.

  From the viewpoint of remarkably exhibiting the effects of the present invention, it is preferable to use one or two or more selected from the group consisting of Kekuromoji, Himekuromoji, and Oobakuromoji, and more preferably Kemomoji. Among the plants of the genus Kuromoji, Kekuromoji is a deciduous shrub distributed in Shikoku, Kyushu, Chugoku region, and the like. The new branch has a green color, and when it becomes a branch of about 1 cm, it has a crust. Kemo moji grows abundantly in Shikoku, such as Kochi Prefecture, and can collect leaves and branches.

  In the present invention, when the chromophore plant is used as it is, its preparation method is not particularly limited. As for the black genus plant, the whole plant or a part of the black genus plant, especially the leaf part, the stem part, the root part, the seed, and the flower part separately one by one or a combination of two or more thereof, or a dried product thereof. The powder can be prepared by appropriately cutting and crushing or grinding. The dried product of the genus Kuromoji or the pulverized product thereof can be used after boiling in hot water, for example. The crushed or pulverized material of the genus Kuromoji can be used after roasting if necessary.

  In the case where a plurality of sites of the genus Kuromoji are used in combination, the combination is not particularly limited. From the viewpoint of obtaining a higher effect of the present invention, the combination of the stem part and the leaf part is preferable for the part of the plant of the genus Cromoji. Moreover, the weight ratio of the stem portion and the leaf portion is not particularly limited, but can be set to 1 to 10: 1 on the basis of the dry weight from the viewpoint of obtaining a higher effect of the present invention. : 1 is preferred, 1-3: 1 is more preferred, and 2-3: 1 is even more preferred.

  The roasting of the genus Kuromoji is performed by a known method using a rotary roaster or the like, and is not particularly limited. The roasting temperature can be, for example, 150 to 400 ° C, preferably 180 to 350 ° C, and more preferably 200 to 330 ° C. The roasting time is appropriately determined depending on conditions such as the roasting temperature, and can be, for example, 5 to 120 minutes, preferably 10 to 90 minutes, and more preferably 15 to 60 minutes.

  In the present invention, in the case of using an extract of the genus Kuromoji, the extraction method is not particularly limited. The extract of the plant of the genus Kuromoji is a dried product of the whole plant or a part of the plant of the genus Kuromoji, particularly leaves, stems, roots, seeds, and flower parts separately or in combination of two or more thereof. The dried product is appropriately cut and extracted from the crushed or pulverized powder with a solvent. The plant of the genus Kuromoji can be easily obtained from a native plant, and a commercially available product can also be used.

  When drying the whole plant or a part of the genus Kuromoji, there is no limitation, but it can be carried out using sun drying, air drying or a dryer. When performing sun-drying, the time required for drying depends on the weather and the like, but can be, for example, 6 hours or more, preferably 1 day or more, more preferably 2 days or more. When using a dryer, it is generally 100 ° C. or lower, preferably 40 ° C. or lower, for example, a rotary dryer, hot air dryer, heat transfer dryer, vacuum dryer, vacuum freeze dryer, A cold air dryer, a vibration dryer, a filter dryer, a vacuum stirring dryer, or the like can be used.

  In the case of using the extract of the genus Kuromoji as a composition for preventing and / or improving lifestyle-related diseases, the extract of the genus Kuromoji may be used as it is, or may be used after appropriately diluted or concentrated. In order to obtain an extract of the genus Kuromoji, it is possible to use a fresh plant, use a genus Kuromoji that has been refrigerated, frozen or dried, or an extract of the extract of the genus Kuromoji. Can also be dissolved or diluted in an appropriate solvent such as water. The concentrate of the extract of the genus Kuromoji is not limited, but a liquid, paste, or mud can be used.

  Examples of the solvent used for the extraction of the black genus plant include water, an organic solvent, and a water-containing organic solvent. By using an appropriate extraction solvent, a water extract, an organic solvent extract or a water-containing organic solvent extract of the genus Cromodium can be obtained. Examples of these extraction solvents include water, alcohol, or a mixture thereof, and water, methanol, ethanol, 1,3-butylene glycol, propylene glycol, glycerin, or a mixture thereof is preferable. From the viewpoint of obtaining higher effects of the present invention, these extraction solvents are more preferably water, methanol, ethanol or hydrous ethanol. The concentration in the case of using methanol is not limited, but from the viewpoint of obtaining higher extraction efficiency, for example, 40% to 99%, preferably 50% to 99%, more preferably 60% to 99%, 70 % To 95%, 80% to 95%. The ethanol concentration in the case of using hydrous ethanol is not limited, but from the viewpoint of obtaining higher extraction efficiency, for example, 40% to 99%, preferably 50% to 99%, more preferably 60% to 99%. , 70% to 95%, 80% to 95%.

  The part of the genus Cromodium genus plant used for extraction is not limited, and examples thereof include a leaf part, a stem part, a root part, a seed, a flower part, and the like. From the viewpoint of obtaining a higher effect of the present invention, the leaf part, stem Parts or combinations thereof are preferred. In this specification, the bark and / or the heartwood may be included in the stem part of the genus Kuromoji.

  The extraction method is not particularly limited. For example, the whole or part of the plant of the genus Chromoji is cut, crushed by a known method such as a mixer, added with an extraction solvent, stirred, and room temperature to heating is performed for a certain extraction time. After the treatment, a method of separating and extracting the extract of the genus Cromodium from the extracted supernatant can be mentioned. Further, the extraction method may be a method in which the plant of the genus Kuromoji is immersed in an extraction solvent and then allowed to stand at room temperature to heating.

  The crushing conditions are not limited, but when the whole plant or part of the sculptured plant is a large scale such as 1 kg to 10 kg, a large vertical cutter mixer or the like can be used. In the case of a small scale of 100 g, a home mixer or the like can be used. For example, when applying crushing conditions on a large scale, it is appropriately changed depending on the hardness of the raw material, moisture content, etc. For example, the crushing time is several tens of seconds to several minutes, and the rotation speed of the mixer is 10 to 3000 rpm. Is possible.

  Moreover, normal conditions can be applied to the extraction conditions, and the extraction conditions are not limited. For example, a method of dipping a dried product of the genus Cromoji in a solvent at 3 to 100 ° C., heating to reflux, or microwave heating is adopted. be able to. The extraction temperature is not limited as long as an appropriate extraction amount can be obtained. For example, when a hydrous alcohol such as 70% ethanol is used, the extraction temperature can be 20 to 100 ° C, preferably 22 to 80 ° C. 60 degreeC is more preferable. Moreover, when water is used, extraction temperature can be 20-100 degreeC, for example, 50-100 degreeC is preferable and 70-100 degreeC is more preferable. The extraction time is not limited as long as an appropriate extraction amount can be obtained. For example, the extraction time can be 5 minutes to 14 days, preferably 10 minutes to 7 days, and more preferably 15 minutes to 5 days. The extraction is usually performed under normal pressure, but can be performed under pressure. About 1L-100L can be used for the addition amount of a solvent with respect to 1 kg of dry weights of the genus Kuromoji.

  It is also possible to increase the extraction efficiency by adding an acid, alkali, or enzyme to the genus Cromodium before adding the extraction solvent.

  A known method can be used for the operation of adding the extraction solvent and stirring, for example, a method of rotating the extraction container containing the cut or pulverized product of the genus Cromoji and the extraction solvent, the extraction container Examples thereof include a method of installing in a magnetic or mechanical stirring device and mixing, a method of shaking the extraction container, and the like.

  The stirring operation can be vibrated by sonication to increase the extraction efficiency. When sonication is performed, there is no limitation. For example, when the whole plant or a part of cut pieces of the genus Cromoji is a large scale such as 1 kg to 10 kg, a commercially available ultrasonic generator may be used. An ultrasonic container can be obtained by installing an extraction container containing a cut product or pulverized product and an extraction solvent and treating with 26 kHz ultrasonic waves for 60 minutes. In the case of a small scale of several tens to several hundreds of grams By treating with 40 kHz ultrasonic waves for 60 minutes, an ultrasonically treated product can be obtained. The temperature condition of the sonication is not limited as long as an appropriate extraction amount is obtained, but can be 2 ° C to 100 ° C, preferably 10 ° C to 70 ° C. As an ultrasonic generator for large scale, for example, UT-12 manufactured by Shinmeidai Kogyo Co., Ltd. can be used. As an ultrasonic generator for small scale, for example, US-3R manufactured by AS ONE Co., Ltd. is used. Can be used.

  In order to increase the extraction efficiency, it is possible to perform multi-stage extraction using the same type or multiple types of extraction solvents. When performing multi-stage extraction, add the same or multiple types of extraction solvent to the residue obtained in the first stage extraction, perform room temperature to heating, and then separate the extract of Cromodium genus plant from the extracted supernatant Can be extracted.

  In the microwave heating, it is possible to appropriately set conditions such as the output of the microwave irradiation apparatus, the wavelength of the microwave, the irradiation time, and the like as long as the activity of the active ingredient of the present invention is not lost. For example, when a microwave of 2450 MHz and 500 W is applied to 100 g of a dried product of the genus Kuromoji, the treatment can be performed for 10 seconds to 10 minutes, preferably 30 seconds to 5 minutes.

  The extracted supernatant can be used as it is as an extract of a plant belonging to the genus Cromodium. Furthermore, the extract of the genus Cromodium can also be separated and extracted from the extract supernatant. In this separation and extraction step, a known method can be employed, and for example, filtration, centrifugation, suction, squeezing, etc. can be performed.

  In the case of performing separation and extraction by filtration, for example, membrane filtration can be performed without limitation. When membrane filtration is performed, for example, the temperature condition can be set to 2 ° C. to 70 ° C., preferably 10 ° C. to 40 ° C., and the membrane pore diameter can be set to 0.1 μm to 10 μm, preferably 0.1 μm to 5 μm. Is possible.

  In the case of separation and extraction by centrifugation, a known device can be used, and examples of the centrifuge include a separation plate type, a cylindrical type, and a decanter type. In the case of performing centrifugation, for example, the temperature condition can be 2 ° C. to 70 ° C., preferably 10 ° C. to 40 ° C., and the number of rotations is 1000 rpm to 10,000 rpm, preferably 1500 rpm to 8000 rpm, more preferably 2000 rpm to 6000 rpm. The centrifugation time can be 10 seconds to 30 minutes, preferably 20 seconds to 20 minutes, and more preferably 30 seconds to 15 minutes.

  For the separation and extraction by pressing, a pressing machine can be used, and a known device can be used as the pressing machine, and examples thereof include a pneumatic pressing machine and a screw pressing machine.

  The extract of the genus Kuromoji can be made into a purified product by subjecting it to further purification treatment before and after dilution or concentration. For the purification treatment, chromatographic methods, ion-exchange chromatographic methods, etc., which are methods known to those skilled in the art, can be used alone or in combination, in addition to the extraction with the above-mentioned solvent.

  In the case of using a chromatographic method, for example, any of column chromatography, high performance liquid chromatography, thin layer chromatography, centrifugal liquid chromatography, etc. using a normal phase or reverse phase carrier or ion exchange resin, or The method of using them in combination is mentioned. The purification conditions such as the carrier and the elution solvent when using the chromatographic method can be appropriately selected in accordance with various chromatographic methods.

  As a ratio of mixing the cut or pulverized product of the genus Kuromoji plant and the extraction solvent, from the viewpoint of obtaining a higher extraction efficiency, for example, when obtaining an aqueous extract, for example, for 1 L of water, The cut or pulverized product of the dried product can be 5 g to 300 g, preferably 10 g to 200 g, more preferably 20 g to 100 g. Moreover, when obtaining a water-containing organic-solvent extract, it is 10g-1kg, Preferably it is 20g-500g, More preferably, it is 30g-200g with respect to 1L of solvents. .

  In the present invention, from the viewpoint of obtaining a higher effect of the present invention, the content of the genus Kuromoji plant, the extract of the genus Kuromoji, in terms of solid content, based on the total amount of the composition for preventing and / or improving lifestyle-related diseases 0.000001-100 wt%, preferably 0.00001-90 wt%, more preferably 0.0001-80 wt%, more preferably 0.001-70 wt%, more preferably 0.01-60 wt% More preferably, it is 0.1 to 50% by weight, more preferably 0.2 to 40% by weight, still more preferably 0.3 to 30% by weight, still more preferably 0.4 to 25% by weight, and still more preferably 0. 5 to 20 wt%, more preferably 0.6 to 15 wt%, more preferably 0.7 to 10 wt%, more preferably 0.8 to 5 wt%, more preferably 0.8 4 wt%, more preferably 0.8 to 3 wt%, more preferably be a 0.8 to 2 wt%.

  In the present invention, the content of the purified product of the genus Kuromoji is 0.000000001 in terms of solid content, based on the total amount of the composition for preventing and / or improving lifestyle-related diseases, from the viewpoint of obtaining a higher effect of the present invention. To 1% by weight, preferably 0.00000001 to 0.9% by weight, and more preferably 0.0000001 to 0.8% by weight.

[Usage]
The inventors of the present invention that the Kuromoji plant or its extract or purified product has an excellent effect on lifestyle-related diseases, such as, but not limited to, obesity improving effect, muscle endurance improving effect, and liver function improving effect. Newly found. The plant of the genus Kuromoji is derived from nature and has been edible since ancient times. Therefore, it is suitable for safe and effective obesity improving use, muscle endurance enhancing use, or liver function improving use. The present invention can be used for pharmaceutical purposes, but can also be used for non-pharmaceutical purposes.

  In the present specification, obesity improvement refers to having an action of lowering lipids in blood, a lipase inhibitory action, or an energy combustion promoting action. Although not limited, lipids in blood can be measured by triglyceride (neutral fat), total cholesterol, LDL-cholesterol called bad cholesterol, free fatty acid, and the like. It is preferred that blood lipids are measured in serum. The composition for preventing and / or improving lifestyle-related diseases of the present invention can reduce triglycerides and cholesterol in blood, and is not limited. However, dyslipidemia (hyperlipidemia), metabolic syndrome, high triglycerides It is also suitable for use in the prevention and / or treatment of blood glucose, hypercholesterolemia, high LDL cholesterolemia, and low HDL cholesterolemia. In addition, the composition for preventing and / or improving lifestyle-related diseases of the present invention is also suitable for foods and beverages directed to patients with these diseases and healthy persons who are aware of the prevention of these diseases.

  Lipase secreted from the pancreas (pancreatic lipase) is a lipid digestive enzyme in the digestive tract. Of the lipids ingested by diet, triglycerides are broken down into fatty acids and monoglycerides by pancreatic lipase and absorbed from the small intestine. A composition that inhibits the activity of pancreatic lipase not only inhibits lipid absorption from the gastrointestinal tract but also suppresses lipid accumulation in the body and is considered to be effective in preventing or improving hyperlipidemia and / or obesity. (See http://wwwsoc.nii.ac.jp/jasso/topics/pdf/topics7_33.pdf “Obesity Study” V01.7 No. 3 p112-114 2001)

  The excess fat and carbohydrates absorbed by the meal are synthesized into neutral fat via free fatty acids and accumulated in fat cells. Therefore, in order to reduce the accumulation of neutral fat and eliminate obesity, it is desirable to efficiently decompose and reduce free fatty acids. As a method for decomposing and reducing free fatty acids, in addition to conversion to chemical energy accompanying the activation of oxygen respiration by exercise (synthesis of ATP), conversion to thermal energy by uncoupling protein (UCP) Is mentioned. In this specification, uncoupling refers to inhibiting the energy obtained by electron transfer from coupling to the ATP synthesis reaction in the oxidative phosphorylation reaction in mitochondria. The uncoupling protein has a function of releasing energy inherently coupled to the ATP synthesis reaction as thermal energy by relaxing a proton gradient in mitochondria due to oxidative phosphorylation. UCP1-5 are known as uncoupling proteins. Of these, it is known that when UCP3 is overexpressed in mice, weight gain and insulin resistance induced by a high-fat diet are suppressed (see (Choi, CS. Et al, 2007, J. Biol.). Clin. Invest., Vol. 117, p. 1995-2003)). UCP3 has also been suggested to be involved in the reduction and elimination of active oxygen (see (Toime, LJ. And Brand, MD., 2010, Free Rad. Biol. Med., Vol. 49, p. 49). 606-11)). Therefore, if the function of UCP3 can be activated, the promotion of the decomposition of free fatty acids and the conversion to thermal energy will lead to the prevention and improvement of obesity and coldness, etc., as well as various types of diabetes related to type 2 diabetes and oxidative stress Expected to lead to improvement of disease.

  In this specification, muscle endurance improvement means improving the ability to work muscles for a certain period of time. In addition, a decrease in muscle endurance means that the ability to work muscles for a certain period of time is reduced. Although not limited, muscle endurance can be measured using the expression level of GLUT4 (Glucose transporter 4), which is associated with an increase in muscle endurance, as an index. The composition for preventing and / or improving lifestyle-related diseases of the present invention can promote an increase in skeletal muscles and increase the expression level of GLUT4. However, the composition is not limited but depends on locomotive syndrome, aging or disease. It is also suitable for use in the reduction of muscle mass, the loss of muscle mass due to lifestyle such as sitting, the reduction of muscle mass due to excessive diet, and the prevention and / or treatment of sarcopenia. In addition, the composition for preventing and / or improving lifestyle-related diseases of the present invention is also suitable for foods and beverages directed to patients with these diseases and healthy persons who are aware of the prevention of these diseases. In another embodiment of the present invention, GLUT4 expression promoting use, muscle sugar uptake promoting use, or in vitro muscle sugar uptake promotion using aspergillus plant or extract or purified product thereof as an active ingredient Applications can be provided.

  In the present specification, liver function improvement means improvement of the circulatory function, excretion function, metabolic function, protection / detoxification function or hematological function of the liver. Further, the decrease in liver function means that the circulation function, excretion function, metabolic function, protection / detoxification function or hematological function of the liver is decreased. Although not limited, liver function may be a GOT (glutamate oxaloacetate transaminase) value, an enzyme such as GPT (glutamate pyruvate transaminase) value, γ-GTP (γ glutamyl transpeptidase) in the blood, or a microscopic view of a liver cell section. It can be measured by observation. The composition for preventing and / or improving lifestyle-related diseases of the present invention can reduce the GOT value and the GPT value, but is not limited, but liver diseases that cause an increase in these values, such as fatty liver, It is also suitable for prophylactic and / or therapeutic agents for alcoholic hepatitis, viral hepatitis such as A, B, and C, cirrhosis, and liver cancer. In addition, the composition for preventing and / or improving lifestyle-related diseases of the present invention is also suitable for foods and beverages directed to patients with these diseases and healthy persons who are aware of the prevention of these diseases.

  Fatty liver medically means a pathological condition in which fat is more than 5% of the total weight of the liver, and liver diseases including this cause death of adult adults in their 40s and 50s in developed countries. Next is a serious disease. Currently, there are few drugs useful for pharmacological treatment of fatty liver, so exercise and diet are recommended, but in fact, treatment of fatty liver by such methods is not so effective. This is a situation where there is an urgent need for the development of effective therapeutic agents. Pathologically, it means a state in which fat vacuoles are observed in 30% or more of 100 cells when hepatocytes are observed with a microscope. When a tissue that is fatty liver is observed with a microscope, an increase in vacuoles in hepatocytes is observed, but the composition for preventing and / or improving lifestyle-related diseases of the present invention is alcohol drinking using a mouse. In the test, vacuole formation in hepatocytes can be suppressed. Although not limited, the composition for preventing and / or improving lifestyle-related diseases of the present invention is suitable for foods and drinks directed to healthy persons who are conscious of preventing and / or improving fatty liver or preventing fatty liver. .

  As another embodiment of the present invention, the composition for preventing and / or improving lifestyle-related diseases of the present invention can lower blood glucose and send blood glucose to muscle cells. It is possible to provide a use for suppressing blood glucose level, using as an active ingredient an extract or purified product. Although not limited, the composition for preventing and / or improving lifestyle-related diseases of the present invention is suitable for foods and drinks directed to healthy persons who are conscious of preventing and / or improving diabetes or preventing diabetes.

[Beverages]
The composition for preventing and / or improving lifestyle-related diseases of the present invention can be blended as one component of food and drink. These foods and drinks are not limited, but can be used as foods and drinks used for obesity improvement, muscle endurance enhancement and / or liver function improvement, for example, in medical institutions such as hospitals. Can be provided for patients. These foods and drinks are not limited, but can be provided as functional drinks or functional foods. These functional foods and drinks are not only medical institutions but also drug stores, convenience stores, supermarkets. Can be provided at department stores, etc. Functional foods and drinks are foods and drinks with medicinal properties that are approved or designated by the government or public organizations, or foods and drinks that display functionality based on the content that the company has reported to the country or the like. Say. Examples of functional foods include foods for specific health use, functional foods for nutrition, functional foods for the elderly, foods for the elderly, and health supplements (balanced nutritional foods and supplements). This includes foods and beverages including packages and containers that display pharmaceutical efficacy or functionality, package inserts, and instruction manuals. Food / beverage products that indicate pharmaceutical efficacy or functionality are also included in the application form to the country.

  In the case of display for the purpose of improving obesity, there is no limitation, but for example, those who are concerned about lipids in blood, those who are obese, those who are concerned about body weight (BMI), those who are concerned about visceral fat, Those who are concerned about subcutaneous fat, those who are concerned about body fat, those who are concerned about lifestyle-related diseases (adult diseases) related to obesity, those who are concerned about the stomach circumference (waist size), those who want to reduce the waist circumference Display for those who want to increase neutral fat, those who want to suppress the increase in neutral fat, those who want to increase total cholesterol and LDL (bad) cholesterol, and those who want to increase HDL (good) cholesterol.

  Display for the purpose of increasing muscle endurance is not limited, but for example, those who want to maintain stamina of strength, those who want to support the decline in muscle strength due to aging, those who want to improve walking ability, daily life or after exercise Those who want to reduce fatigue, those who feel easy fatigue or fatigue (related to muscle loss), those who are concerned about coldness (related to muscle loss), (related to muscle loss) Display for those who are worried about poor posture such as stoops, those who have pain in knees when walking or running, those who want to improve aerobic exercise ability, those who want to improve their daily work ability, etc. It is done.

  Display for the purpose of improving liver function is not limited. For example, those who want to maintain healthy liver function, those who are concerned about hangover or fatigue from drinking, those who are concerned about GOT or GPT values, fatigue, etc. Display for those who feel sensation, those who feel fatigue, anorexia, and those who are concerned about lifestyle-related diseases (adult diseases) regarding liver function.

  In the case of display for the purpose of improving blood glucose level, there is no limitation, but for example, those who are high in blood glucose level, those who are concerned about blood glucose level, those who are concerned about blood glucose level after eating, Display for those who want to be relaxed.

  Examples of food include all foods, such as cereals, potatoes, seafood, meats, eggs, fats and oils, milk, vegetables, beans, fruits, sugars, seaweeds, confectionery, seasonings And cooked processed foods.

  The seasoned processed food is not limited, but for example, processed fishery products such as chikuwa and kamaboko; processed livestock products such as ham and sausage; confectionery such as cookies, biscuits, snacks, chocolate, cakes; soba, udon, raw noodles Noodles such as Chinese noodles and pasta; bread such as bread and confectionery bread; fermented processed foods such as natto and miso; soybean foods such as tofu and okara; pickles such as pickles and pickles; marine products; processed meats and vegetables; Canned fruits and the like; dairy products such as butter, margarine, yogurt, cheese and milk; frozen dessert foods such as ice cream and sherbet.

  Examples of beverages include, but are not limited to, all beverages, for example, beverages that use the black genus plant itself as tea, dried roasts as substitutes for tea leaves, fruit juice beverages, fruit juice 100% beverages, and low fruit juice beverages , Fruit drinks, vegetable juices, flavored drinks, fruit drinks such as fruit drinks for dilution; carbonated drinks; preferences for coffee, coffee drinks, soft drinks with coffee, cocoa drinks, tea, green tea, matcha, oolong tea, barley tea, roasted tea, etc. Beverages; Vinegar drinks; Soft drinks such as sports drinks; Milk; Milk drinks; Milk drinks; Lactic drinks; Lactic acid bacteria drinks; Soy drinks such as soy milk and prepared soy milk; Alcoholic drinks such as beer, sake, shochu, liqueur, wine A nutritional drink containing taurine, royal jelly, amino acids, vitamins, minerals, iron, and the like.

  Although there is no restriction | limiting in the time which applies this invention to food / beverage products, For example, in the manufacturing process of food / beverage products, it can apply before and after a process process, a cooking process, a heating process, a preservation | save process, etc. For example, in the processing step and cooking step, the composition for preventing and / or improving lifestyle-related diseases of the present invention can be included in the raw material. The application method can be appropriately changed according to the type of food and drink, the form of the raw material, and the like, and various methods such as mixing, addition, application, spraying, and immersion can be adopted.

  When the composition for preventing and / or improving lifestyle-related diseases of the present invention is applied to beverages, in one embodiment, a tea beverage is preferable. Although it does not limit, the leaf part and / or stem part of the dried genus Cromoji plant are cut | disconnected by a well-known method, Then, it crushes or grind | pulverizes and it is set as a powdery material. It can be sold as a tea beverage in the form of a powder of a black genus plant. Further, if necessary, the powdered plant of the genus Kuromoji is roasted. It is also possible to sell the powder of the genus Kuromoji as a tea beverage in a roasted state. For example, a powder or roasted product of the genus Kuromoji can be sold in a form in which an appropriate amount is enclosed in a tea bag. The roasted genus Kuromoji is drunk by boiling it in hot water.

  In the case where the composition for preventing and / or improving lifestyle-related diseases of the present invention is applied to a tea beverage, in another embodiment, the stem part and the leaf part of the genus Kuromoji are 1 to 3: 1 based on the dry weight. It is possible to use it as a tea beverage by using it in a weight ratio, drying and grinding, and roasting.

  Moreover, the said drink and other tea drinks, and a black-skinned genus plant or its extract can be used as a mixture, or it can be diluted with water etc. as juice, a drink agent, etc. Furthermore, it can be used as a concentrated product of the above mixture, powdered tea powdered by spray drying, lyophilized instant tea, or the like. In that case, hot water can be poured into, for example, powdered tea or instant tea. Further, for example, a mixture of a tea preparation such as green tea or black tea and a genus Cromoji plant or an extract thereof can be used as it is as a tea leaf for beverages. In that case, hot water can be poured into a mixed tea leaf of a tea preparation and a plant of the genus Cromoji or an extract thereof in the same manner as a drink for general Japanese tea or black tea.

  When the composition for preventing and / or improving lifestyle-related diseases of the present invention is applied to foods and drinks, an auxiliary agent used in ordinary foods and beverages is appropriately blended within a range not impairing the effects of the present invention. Is possible. Examples of such auxiliaries include glucose, sucrose, fructose, oligosaccharide, starch syrup, maltose, maltose, xylitol, sorbitol, aspartame, sucralose, stevioside, rubusoside, corn syrup, lactose, citric acid, tartaric acid, malic acid Succinic acid, lactic acid, L-ascorbic acid, dL-α-tocophenol, sodium erythorbate, glycerin, glycerin fatty acid ester, propylene glycol, polyglycerin fatty acid ester, sucrose fatty acid ester, propylene glycol fatty acid, sorbitan fatty acid ester, ester Gum arabic, casein, pectin, gelatin, agar, carrageenan, vitamins B, vitamins C, nicotinamide, calcium pantothenate, calcium salts, amino acids, pigments, Fee, preservatives, and the like.

  When the composition for preventing and / or improving lifestyle-related diseases of the present invention is applied to foods and drinks, the content of the plant of the genus Cromodium or its extract is not limited, but may be human or animal in terms of solid content. For example, it is generally 0.00001 to 5000 mg / kg body weight, preferably 0.01 to 200 mg / kg body weight, more preferably 0.1 to 100 mg / kg body weight per day.

  The pH in the case of applying the composition for preventing and / or improving lifestyle-related diseases of the present invention to a beverage is appropriately adjusted depending on the type of the beverage, but for example, in the case of a tea beverage, the pH is 3.5 to 7. 5 to 3.8, preferably 3.8 to 7.2, more preferably 4.0 to 7.0, and even more preferably 4.5 to 6.5. The pH of the beverage can be adjusted, for example, by appropriately blending sodium bicarbonate, citric acid and the like.

[Pharmaceuticals, Quasi-drugs]
The composition for preventing and / or improving lifestyle-related diseases of the present invention can be formulated into an appropriate form and administered to humans or animals in any dosage form when it is used as a pharmaceutical or quasi-drug. Examples of the dosage form include, but are not limited to, oral, transdermal, enteral, transmucosal, and injection. From the viewpoint of remarkably exhibiting the effects of the present invention, the administration form is preferably oral administration.

  When the composition for preventing and / or improving lifestyle-related diseases of the present invention is orally administered, the composition for preventing and / or improving lifestyle-related diseases of the present invention includes, for example, tablets, capsules, granules, and syrups. It is formulated into a dosage form such as powder, film, drop, jelly, or semi-solid purine by a known method. In the case of oral administration in the form of a film, drop, jelly, semi-solid purine, etc., the composition for preventing and / or improving lifestyle-related diseases of the present invention may be taken without water. Is possible. Alternatively, it is possible to orally administer a dry powder of a plant of the genus Cromodium or an extract or purified product thereof as a herbal medicine or a herbal medicine preparation.

  In the case of parenteral administration, for example, it can be formulated by a known method into dosage forms such as intravenous injection, intramuscular injection, transdermal absorption agent, inhalant, suppository, eye drop, nasal drop and the like. is there.

  As one mode of administration, a known drug is used for the purpose of enhancing the stability and bioavailability of a plant of the genus Cromodium or an extract or purified product thereof, improving patient compliance, or a combination thereof. Using the delivery system, it is possible to deliver the composition for preventing and / or improving the lifestyle-related disease of the present invention to the absorption site.

  Examples of drug delivery systems include, but are not limited to, polymers such as cellulose, dextran, starch, polyvinyl alcohol, acetylated or methacrylated polymers, polylactic acid and polyglycolic acid and block copolymers thereof, and polyethylene glycol. Examples thereof include a method using a transport protein such as albumin, and other methods using micelles, liposomes, microspheres, nanoparticles, composite emulsions, dendrimers and the like. These drug delivery systems are used not only for the purpose of transporting the composition for preventing and / or improving the lifestyle-related disease of the present invention to a target site such as an absorption site, but also for preventing and / or improving the lifestyle-related disease of the present invention in a timely manner. It can also be used for the purpose of controlled release to release the composition.

  The composition for preventing and / or improving lifestyle-related diseases according to the present invention is a liquid that contains an excipient, a lubricant, a binder, a disintegrant, etc. In the preparation, a solvent, a solubilizing agent, a suspending agent, a tonicity agent, a buffering agent, a soothing agent and the like can be appropriately blended.

  Examples of the excipient include lactose, sucrose, D-mannitol, glucose, starch, calcium carbonate, kaolin, crystalline cellulose, and light anhydrous silicic acid.

  Preferable examples of the lubricant include magnesium stearate, calcium stearate, talc, purified talc, colloidal silica, borax, polyethylene glycol and the like.

  Examples of the binder include water, ethanol, propanol, simple syrup, glucose solution, starch solution, gelatin solution, bound cellulose, sucrose, D-mannitol, dextrin, carboxymethylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, hydroxypropyl starch. , Methylcellulose, ethylcellulose, shellac, calcium phosphate, polyvinylpyrrolidone and the like.

  Examples of the disintegrant include starch, dry starch, carboxymethyl cellulose, carboxymethyl cellulose calcium, carmellose calcium, croscarmellose sodium, carboxymethyl starch sodium, sodium alginate, agar powder, sodium bicarbonate, calcium carbonate, sodium lauryl sulfate, Examples include stearic acid monoglyceride and lactose.

  Examples of the solvent include water for injection, alcohol, propylene glycol, macrogol, sesame oil, corn oil and the like.

  Examples of the solubilizer include polyethylene glycol, propylene glycol, D-mannitol, benzyl benzoate, ethanol, trisaminomethane, cholesterol, triethanolamine, sodium carbonate, sodium citrate and the like.

  Examples of the suspending agent include surfactants such as stearyltriethanolamine, sodium lauryl sulfate, laurylaminopropionic acid, lecithin, benzalkonium chloride, benzethonium chloride, and glyceryl monostearate, for example, polyvinyl alcohol, polyvinyl Examples thereof include hydrophilic polymers such as pyrrolidone, sodium carboxymethylcellulose, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, and hydroxypropylcellulose.

  Examples of the isotonic agent include sodium chloride, glycerin, D-mannitol and the like.

  Examples of the buffer include buffer solutions such as phosphate, acetate, carbonate, citrate, and the like.

  Examples of soothing agents include benzyl alcohol.

  Examples of the preservative include p-hydroxybenzoates, chlorobutanol, benzyl alcohol, phenethyl alcohol, dehydroacetic acid, sorbic acid, and the like.

  The effective dose of the plant of the genus Cromodium or the extract thereof when orally administering the composition for preventing and / or improving lifestyle-related diseases of the present invention is not limited, but it is human and animal in terms of solid content In general, it is 0.00001 to 5000 mg / kg body weight per day, preferably 0.01 to 200 mg / kg body weight, more preferably 0.1 to 100 mg / kg body weight. The frequency of administration is usually about 1 to 4 times a day, but can be appropriately adjusted depending on the administration route and the like.

  Next, the present invention will be specifically described with reference to test examples and examples, but the present invention is not limited to the following examples.

(Example 1) Extraction method 1 from a dry ground product with a water-containing organic solvent 1
20 g of each dry pulverized product of leaves and stems of L. serica was dispersed in 300 ml of 70% ethanol aqueous solution, stirred, and extracted at room temperature for 24 hours. After the extraction supernatant was filtered off, the residue was again dispersed in a 70% aqueous ethanol solution. After heating to reflux, the extraction supernatant was filtered off. Further, the residue was dispersed in a 70% aqueous ethanol solution, and after 1 hour of sonication, the extracted supernatants were separated by filtration, and the combined supernatants were successively concentrated under reduced pressure, dried under reduced pressure in a nitrogen gas atmosphere, and freeze-dried. Extract 1 (6 g) from the leaves and Extract 2 (6 g) from the stems of kerosene were obtained.

(Example 2) Extraction method with water from dry pulverized product Add 1.5 L of water to 100 g of dry pulverized leaf of L. serica, pulverize with a mixer, and then stir and apply ultrasonic waves. The extract was filtered and dehydrated to obtain an extract (2 L) together with water. Further, the extract 3 (20 g) was obtained by successively performing vacuum concentration, vacuum drying and lyophilization under a nitrogen gas atmosphere.

(Example 3) Extraction method by squeezing Add 1 L of water to a mixture (1 kg) of fresh leaves and stems of L. serica, crush it with a mixer, stir and apply ultrasonic waves, and filter the extraction supernatant. Separately, by washing and dehydrating, an extract (2 L) was obtained together with water. Further, the extract 4 (20 g) was obtained by successively performing vacuum concentration, vacuum drying and lyophilization under a nitrogen gas atmosphere.

(Example 4) Extraction method by pressing 1 L of water is added to a mixture (1 kg) of fresh leaves and stems of L. serica, pulverized with a mixer, stirred and ultrasonically applied, and the extraction supernatant is filtered off. And after dehydration, the extract (1.5L) was obtained by pressing. Further, the extract 5 (25 g) was obtained by successively performing vacuum concentration, vacuum drying and lyophilization under a nitrogen gas atmosphere.

(Example 5) Method for producing dry powder Fresh leaves and raw stems (1 kg) of kerosene mushroom (L. sericea) were dried overnight in a cool air dryer at 40 ° C. and then finely pulverized with a pulverizer, and then dried keke moji powder (100 g) Got.

(Example 6) Extraction method using Psyllium moji Add 10 mL of water to 1 g of dry pulverized stalks of L. umbelate var. Membrancea (Maxim) Moxa), and then extract for 10 minutes. The extract was obtained by filtration and dehydration. 10 mL of water was again added to the residue, extraction was performed for 10 minutes, and then the supernatant was filtered and dehydrated. The extract 6 obtained by mixing with the previously obtained extract and making up to 20 mL with water was obtained (50 mg equivalent / ml).

(Example 7) Extraction method by microwave Add 20 mL of water to 1 g of dry pulverized leaves and stems of L. sericia, seal in a polypropylene container, and heat for 1 minute in a microwave oven (2450 MHz, 500 W). The extract supernatant was filtered and dehydrated to obtain extract 7 (20 mL) together with water. Extract 8 was obtained in the same manner with a heating time of 5 minutes.

(Example 8) Extraction method 1 from hot pulverized product with hot water
By adding 1.5 L of hot water to 100 g of dry pulverized leaves and stems of L. sericea and filtering the extract supernatant, an extract (2 L) was obtained together with water. Further, the extract 9 (15 g) was obtained by successively performing vacuum concentration, vacuum drying and lyophilization under a nitrogen gas atmosphere.

(Example 9) Extraction method with hot water from fresh leaves and live stems 1 L of hot water was added to a mixture (1 kg) of fresh leaves and fresh stems of L. serica, and the extract supernatant was filtered to remove water. And an extract (1.5 L) was obtained. Further, the extract 10 (10 g) was obtained by successively vacuum concentration, vacuum drying and lyophilization under a nitrogen gas atmosphere.

(Example 10) Extraction method 2 from a dry pulverized product with a water-containing organic solvent 2
20 g of a dry pulverized product of stems (including bark and heartwood) and 20 g of a dried pulverized product of leaves were dispersed and stirred in 300 ml of 80% methanol, and extracted at room temperature for 24 hours. After the extraction supernatant was filtered off, the residue was again dispersed in 80% methanol. After heating to reflux, the extraction supernatant was filtered off. Further, the residue was dispersed in a 70% aqueous ethanol solution, and after 1 hour of sonication, the extracted supernatants were separated by filtration, and the combined supernatants were successively concentrated under reduced pressure, dried under reduced pressure in a nitrogen gas atmosphere, and freeze-dried. Extract 11 (1.5 g) from stalks and Extract 12 (5 g) from leaves of kerosene were obtained. These extracts 11 and 12 were mixed at a weight ratio of 7: 3 based on the dry weight to obtain an extract 13.

(Example 11) Extraction method 2 from dry pulverized product with hot water
Extraction liquid (2 L) together with water by adding 1.5 L of hot water to 100 g of each dried pulverized product of L. sericea stem (including bark and heartwood) and leaves, and filtering the extract supernatant. Got. Further, vacuum concentration, vacuum drying and lyophilization were successively carried out under a nitrogen gas atmosphere to obtain an extract 14 (6 g) from the stems of kerosene and an extract 15 (6 g) from the leaves of kerosene. These extracts 14 and 15 were mixed to obtain an extract 16.

(Animals and food)
Seven week old natural weight gain mice (C57BL / 6J male mice, average weight 23.1 g) were purchased from Charles River. The animals were housed under conditions of a light-dark cycle of room temperature 24 ± 3 ° C., 12/12 h (AM.7: 00-PM.7: 00). Mice were given free access to water and food. The mice were then assigned to 3 groups of 10 animals (low-fat diet group, high-fat diet group, or high-fat cromozi diet group) according to body weight, and the following diets were freely fed for 36 days (test) period).

  The details of the diet in each group are as shown in Table 1. In addition, the unit of the numerical value in Table 1 is weight%. The chromophore extract in Table 1 was prepared so as to contain 1% of the chromophore plant extract 13 obtained in Example 10 in terms of solid content.

(Test Example 1) Evaluation test of obesity-improving effect After completion of the above test period (animal and diet), serum triglycerides and cholesterol were evaluated. After the test period, blood was collected under ether anesthesia and sacrificed, and the weights of various organs were measured. In addition, serum was prepared and stored at −30 ° C. until analysis (Test Example 1-1) Measurement of serum triglyceride Triglyceride in serum was triglyceride E-Test Wako kit (manufactured by Wako Pure Chemical Industries, Ltd.). Quantified according to manufacturer's instructions. The results of blood triglycerides are shown in FIG. The vertical axis of the graph represents the concentration per serum in mg / dL.

  As shown in FIG. 1, as a result of measuring triglycerides in serum, it was found that the triglycerides in serum were significantly reduced in the high-fat cromozi diet group as compared to the high-fat diet group. Surprisingly, it has been found that the triglycerides in the serum of the high-fat cromozi diet group are further reduced than in the low-fat diet group.

(Test Example 1-2) Measurement of serum cholesterol Cholesterol in serum was quantified using a cholesterol E-Test Wako kit (manufactured by Wako Pure Chemical Industries, Ltd.) according to the manufacturer's instructions. The result of blood cholesterol is shown in FIG. In FIG. 2, the vertical axis of the graph represents the concentration per serum in mg / dL.

  As shown in FIG. 2, as a result of measuring cholesterol in the serum, it was found that the cholesterol in the serum was significantly reduced in the high-fat chromophore group compared with the high-fat diet group. Surprisingly, it was found that the cholesterol in the serum of the high fat chromophore group was reduced to the same extent as in the low fat diet group.

(Test Example 1-3) Lipase Inhibitory Activity Evaluation Test Extracts A, B, C of Cromodium Plants Obtained in Example 8-2, and Extracts 11, 12 of Cromodium Plants Obtained in Example 10 13 was dissolved in dimethyl sulfoxide (DMSO) to a concentration of 10 mg / mL and subjected to a lipase inhibitory activity evaluation test. For the evaluation test, porcine pancreatic lipase (manufactured by Sigma-Aldrich) and lipase kit S (manufactured by Dainippon Pharmaceutical) were used. The measuring method was carried out by using a 96-well microplate with a partial modification of the kit method. That is, 15 μL of a sample solution containing an extract, 4 μL of an enzyme solution (porcine pancreatic lipase 0.05 mg / mL, 125 mmol / L Tris-HCl (pH 7.5)), a coloring solution (0.1 mg / mL 5,5′- After adding 50 μL of dithiobis (buffer solution containing 2-nitrobenzoic acid) and mixing, preheat for 5 minutes at 30 ° C., substrate solution (6.69 mg / mL dimercaprol tributyrate + 5.73 mg / mL sodium dodecyl sulfate) ) After 5 μL was added and mixed, the mixture was heated at 30 ° C. for 30 minutes under light shielding. After adding a reaction stop solution, the absorbance at 412 nm was measured with a microplate reader. In addition, in the control of each sample, DMSO was added as a sample solution, and the same operation was performed. The results of the lipase inhibitory activity evaluation test are shown in FIG.

In FIG. 3, the vertical axis represents the average inhibition rate (%). Three samples were tested for each extract, and the inhibition rate (%) was calculated by the following formula 1.
[Formula 1]
Inhibition rate (%) = 100− (test group−blank) / control × 100

  As shown in FIG. 3, as a result of measuring the lipase inhibitory activity of the extract of the chromophore extract, the sample group of the extract of the genus Chromody exhibits a high lipase inhibitory activity compared to the negative control not containing the extract of the genus Crochromium. As a positive control, green tea extract (Sanphenon 90S, Taiyo Kagaku Co., Ltd.) was inhibited to the same extent as a sample (camellia extract in FIG. 3) dissolved in dimethyl sulfoxide (DMSO) to a concentration of 2.5 mg / mL. It became clear to show activity.

(Test Example 2) Evaluation test of muscle endurance effect (Test Example 2-1) Measurement of body weight During the above test period (animal and food), the body weight was measured every week. The measurement results of body weight are shown in FIG. In FIG. 4, the vertical axis of the graph shows the average weight of each group in g.

  As shown in FIG. 4, the body weight increased over time in the low-fat diet group, the high-fat diet group, and the high-fat cromozi diet group. The amount of weight gain of mice was less affected by the addition of the extract of the genus Cromodium, and the high-fat chromophore group was slightly increased compared to the high-fat diet group.

(Test Example 2-2) Measurement of visceral fat mass The visceral fat mass was measured by a gravimetric method. For each organ of the liver, accessory testicle, kidney, mesentery, and brain, the amount of fat produced in and / or around them and the total amount of visceral fat in the whole body were measured. The result of measuring the amount of fat in the liver by measuring the liver weight is shown in FIG. The result of measuring the amount of fat in the testicles by measuring the weight of the testicles is shown in FIG. The result of measuring the amount of fat around the kidney by measuring the kidney weight is shown in FIG. The results of measuring the mesenteric fat mass by measuring the mesenteric weight are shown in FIG. The result of measuring the amount of fat in the brain by measuring the brain weight is shown in FIG. The result about the total amount of visceral fat in the whole body is shown in FIG. 5-10, the vertical axis | shaft of a graph shows the average of fat weight by g.

  As shown in FIGS. 5 to 10, when the visceral fat mass of each organ and whole body was compared, no significant difference was found between the high fat chromophore diet group and the high fat diet group, and the cause of weight gain was the fat mass It was suggested that it was due to an increase in skeletal muscle, not an increase.

(Test Example 2-3) Measurement of expression levels of GLUT4 gene and UCP-3 gene Therefore, the extract 11 or 12 of the genus Cromoji obtained in Example 10 was administered to C2C12 cells, which are mouse myocytes, The expression levels of the GLUT4 (glucose transporter 4) gene involved in muscle endurance and the UCP-3 gene (uncoupling protein-3) involved in energy production were quantified by the qPCR method.

Specifically, mouse-derived skeletal muscle cells (C2C12) were used. Cells were cultured using Dulbecco's modified Eagle medium DMEM (4.5 g / L glucose concentration) at 37 ° C. and 5% CO 2 concentration. When the cells became 90% confluent, the number of cells was 1 × 10 6 in a 96-well culture plate. ^The cells were cultured to ⁵ / mL. The medium was changed every 2 days, and the culture was continued until it became confluent (about 5 days). Thereafter, the cells were cultured in DMEM (1.0 g / L glucose concentration) for 3 days to confirm differentiation, and then the medium was replaced with a medium supplemented with chromophore extract and cultured for 2 days. The medium added with black moji extract was prepared by dissolving the black moji extract (extract 11 or 12) with dimethyl sulfoxide to 10 μg / mL and adding 0.5% to the medium.

Thereafter, the cells were suspended using a cell lysate (CellAmp ^™ Direct RNA Prep Kit for RT-PCR: manufactured by Takara Bio Inc.), and RNA was extracted. From this, cDNA was prepared (PrimeScript ^™ RT reagent Kit: manufactured by Takara Bio Inc.), and further, Glucose transporter-4 (Glut-4) and Uncoupling protein-3 by TaqMan probe method (Probe qPCR Mix: manufactured by Takara Bio Inc.). The expression level of (UCP-3) was quantified, and the ratio of the expression level of each gene to the expression level of the β-actin gene (indicated by “C” in the figure) was expressed using β-actin as a housekeeping gene. . The results of the expression level of the GLUT4 gene or the expression level of the UCP-3 gene are shown in FIG. 11 and FIG. 12, respectively.

  From the results shown in FIG. 11 and FIG. 12, it was revealed that the expression levels of both genes were significantly increased in the high-fat chromophore diet group compared to the high-fat diet group. The protein produced by GLUT4 is a channel involved in intracellular transport of glucose, moves to the cell surface by insulin stimulation and muscle contraction, and sends glucose in blood to muscle cells. Therefore, when GLUT4 increases, the amount of glucose taken into muscle cells increases, and ATP in mitochondria increases, leading to an increase in muscle endurance. UCP-3 also has the effect of inhibiting the energy obtained by electron transfer from coupling to the ATP synthesis reaction in the oxidative phosphorylation reaction in mitochondria. By doing so, it has a function of releasing energy that is originally conjugated to the ATP synthesis reaction as thermal energy. This is considered to have an effect of preventing obesity due to excessive energy uptake. In other words, it has been clarified that the black moji extract exerts anti-obesity, endurance improvement, and consequently muscle mass increase effect by activating these gene expressions.

(Test Example 2-4) Measurement of glucose in serum After completion of the above test period (animal and diet), glucose in serum was evaluated. Glucose in serum was quantified using a glucose CII-Test Wako kit (manufactured by Wako Pure Chemical Industries) according to the manufacturer's instructions. The result of serum glucose is shown in FIG. In FIG. 13, the vertical axis of the graph represents the concentration per serum in mg / dL.

  As shown in FIG. 13, as a result of measuring glucose in the serum, it was found that the glucose in the serum was significantly reduced in the high fat chromophore diet group as compared to the high fat diet group. This is thought to be due to an increase in the amount of glucose taken into muscle cells.

(Test Example 3) Evaluation test of liver function improvement effect (Test Example 3-1) Measurement of serum GOT value and GPT value After completion of the above test period (animal and food), mouse liver weight and serum GOT The value and the GPT value were evaluated. As described above, the measurement result of the liver weight is shown in FIG. GOT and GPT values in serum were quantified using transaminase CII test Wako (manufactured by Wako Pure Chemical Industries, Ltd.) according to the manufacturer's instructions. The results of GOT value and GPT value in serum are shown in FIG. 14 and FIG. 15, respectively. In FIG. 14 or FIG. 15, the vertical axis of the graph indicates the unit (U).

  As shown in FIG. 14 or FIG. 15, as a result of measuring the GOT value and GPT value, which are indicators of liver function, both the GOT value and the GPT value are significantly decreased in the high fat chromodies group compared to the high fat group. It was recognized that

(Test Example 3-2) Evaluation of fatty liver in alcohol load test In order to examine in detail the effect of improving liver function, a feeding test in which 10% alcohol was consumed was performed. In the group designated as the control diet group, the high fat diet and water listed in Table 1 were given. In a group designated as an alcohol intake group, the high fat diet shown in Table 1 and 10% alcohol were given. In a group designated as an alcohol-ingested chromophore group, the high-fat chromophore diet shown in Table 1 and 10% alcohol were given. Other conditions are the same as above (animal and food). Observation of a section of the right lobe of the liver of the mouse after the end of the test period (HE staining) was performed. The result is shown in FIG.

  As shown in FIG. 16, it is clear that the alcohol-ingested chromodie group has less vacuole formation in the hepatocytes than the alcohol-ingested group, and is comparable to the control group that has not ingested alcohol. It was. This indicates that fatty liver formation due to alcohol is suppressed.

  By the same method as in Test Example 3-1, the GOT value and the GPT value, which are indicators of liver function, were measured for the control food group, the alcohol intake group, and the alcohol intake chromophore diet group. The results of serum GOT and GPT values are shown in FIG. 17 and FIG. 18, respectively. In FIG. 17 or FIG. 18, the vertical axis of the graph indicates the unit (U).

  As shown in FIG. 17 or FIG. 18, as a result of measuring the GOT value and GPT value, which are indicators of liver function, both the GOT value and GPT value are significantly decreased in the alcohol-ingested chromophore group compared to the alcohol-ingested group. Was recognized.

(Prescription example)
The unit of the numerical value in each of the following formulation examples is indicated by “% by weight” where the total amount is 100.

(Prescription Example 1) Composition (tablet) for prevention and / or improvement of lifestyle-related diseases
Using the extracts obtained in the above-mentioned Examples, tablets for preventing and / or improving lifestyle-related diseases having the following composition can be produced by a conventional method.

(Prescription Example 2) Composition for preventing and / or improving lifestyle-related diseases (granule)
Using the extract obtained in the above Examples, the following composition can be mixed and stirred to prepare uniformly, and granules can be obtained with a fluidized bed granulator. Granules can be taken with beverages.

(Prescription Example 3) Composition for preventing and / or improving lifestyle-related diseases (capsule)
Using the extract obtained in the above Examples, the following composition can be mixed and stirred to prepare uniformly to obtain a capsule powder for use in a capsule.

(Prescription Example 4) Beverage for preventing and / or improving lifestyle-related diseases A beverage for preventing and / or improving lifestyle-related diseases having the following composition according to a conventional method, using the extract obtained in the above-mentioned examples. Can be manufactured.

(Prescription Example 5) Chewing gum for prevention and / or improvement of lifestyle-related diseases Chewing gum for prevention and / or improvement of lifestyle-related diseases having the following composition by a conventional method using the extract obtained in the above-mentioned examples. Can be manufactured.

Claims (8)

  A composition for preventing and / or improving lifestyle-related diseases, comprising a plant of the genus Kuromoji or an extract thereof.   The composition for preventing and / or improving lifestyle-related diseases according to claim 1, wherein the lifestyle-related diseases are obesity, muscle endurance reduction or liver function decline.   The life according to claim 1 or 2, wherein the genus Kuromoji is derived from one or more selected from the group consisting of whole plants or leaves, stems, roots, seeds, and flowers of the genus Kuromoji. A composition for preventing and / or improving habitual disease.   The varieties of the genus Kuromoji are 1 or 2 or more selected from the group consisting of Kekuromoji, Himekuromoji, and Oobakuromoji, for prevention and / or improvement of lifestyle-related diseases according to any one of claims 1 to 3. Composition.   The composition for lifestyle-related disease prevention and / or improvement of any one of Claims 1-4 whose said extract is a water extract, an organic-solvent extract, or a water-containing organic-solvent extract.   The composition for preventing and / or improving lifestyle-related diseases according to any one of claims 1 to 5, which is administered orally, intravenously, by inhalation or transdermally.   The composition for lifestyle-related disease prevention and / or improvement of any one of Claims 1-6 which is food-drinks, a quasi-drug, or a pharmaceutical.   The composition for preventing and / or improving lifestyle-related diseases according to any one of claims 1 to 7, wherein the food or drink is a tea beverage.