KR101328413B1 - An extracting method of ginseng by high pressure/enzyme decomposition - Google Patents

Abstract

In the present invention, 20% to 30% by weight of ginseng is added to the weight of water, 0.5% to 1.2% by weight of the enzyme is added to the high pressure reactor, and the high pressure reactor is adjusted to a temperature of 55 ° C to 60 ° C. The present invention provides a method for extracting useful components of ginseng by autoclaving to extract useful components of ginseng by maintaining 100 to 200 MPa for 24 to 48 hours.

Description

Extraction method of useful ingredients of ginseng by high pressure / enzymatic digestion {AN EXTRACTING METHOD OF GINSENG BY HIGH PRESSURE / ENZYME DECOMPOSITION}

The present invention relates to a method for extracting useful components of ginseng by high pressure / enzymatic digestion. More specifically, the present invention relates to a method for producing ginseng drink by extracting more than 98% saponin content by high pressure / enzymatic digestion from ginseng, red ginseng, and camphor ginseng.

Korean Ginseng ( Panax ginseng CA Meyer, family Araliaceae) has been used as a medicinal plant in the Orient for thousands of years, and is regarded as the most valuable medicinal plant in Korea, Japan and China. The representative pharmacological action of ginseng is used for the purpose of treating cancer, heart disease, aging delay and adult disease by providing activity promoting effect on the central nervous system, cardiovascular system, endocrine system and immune system. Ginseng currently has anti-cancer effect, anti-cancer and anti-cancer activity, immune-enhancing effect, anti-diabetic effect, hepatic function-enhancing effect, anti-fatigue and anti-stress effect, improvement of menopausal disorders, improvement of male sexual dysfunction, AIDS virus As it is recognized as the best natural medicine that exhibits various pharmacological effects such as anti-proliferative effect, antioxidant activity and anti-aging effect, the global market demand is increasing. The pharmacological action of the pharmacologically active component of ginseng is summarized in [Table 1].

Pharmacological action according to the types of pharmacologically active ingredients Pharmacologically active ingredient Pharmacological action material
G-Rb1, G-Rg1, G-Rg2
Learning function improvement, memory decline Saito et al. 1988
Zhang et al. 1990 Panaxatriol-based saponins
40% Ethanol Ginseng Extract
Improved intellectual work performance Park et al. 1994
Dangelo 1996
G-Rh2, polyacetylene compound
(panaxydol, panaxynol, panaxy-
triol)

Cancer cell proliferation inhibition Ahn et al. 1987
Matsunaga et al. 1990
Kikuchi et al. 1991
Odasshima et al. 1979
Hwang 1993 G-R3, G-Rb2 Cancer cell metastasis inhibition Mochizuki et al. 1995 G-Rh2, panaxytriol Anticancer activity Kubo et al. 1992 G-Rh2, polysaccharide Immune function Ahn et al. 1987
G-Rb2, acidic peptide, adenosine, pyroglutamic acid
Antidiabetic Yokozawa et al. 1985
Okuda et al. 1990
Takaku et al. 1990
Ando et al. 1979

G-Rb1, G-Rg1, G-Rf, G-Ro,
Ginseng Extract

Hepatic hyperactivity Song et al. 1990
Mizoguchi et al. 1988
Huh et al. 1988
Lee et al. 1984
Okamura et al. 1994
Matsuda et al. 1991
G-Rg1
Anti-fatigue and antistress efficacy Saito et al. 1984
Kim 1979
Kaneko et al. 1996
G-Rg1, Red Ginseng Extract
Improve male sexual dysfunction Saito et al. 1984
Choi et al. 1995
Joe Saponin
AIDS virus growth inhibition Shin et al. 1993
Cho et al. 1993
Polyphenols and polyacetylene compounds
Antioxidant and Anti Aging Han et al. 1985
Kim et al. 1988
Chung et al. 1993
Wang et al. 1994

An important ingredient in ginseng is ginsenoside glycoside, which has different characteristics from saponins obtained from other plants. In other words, ginseng saponin is a triterpenoid dammarane saponin, which exists only in the plant of ginseng, and its pharmacological action is completely different from that of other medicinal plants. Looking at the molecular weight of the typical ginsenosides are as follows.

Ginsenoside-Rg ₁ (C ₄₂ H ₇₂ O ₁₄ 2H ₂ O): 837

Ginsenoside-Rb ₁ (C ₅₄ H ₉₂ O ₂₃ 3H ₂ O): 1163

Ginsenoside-rf (C ₄₂ H ₇₂ O ₁₄ 2H ₂ O): 837

Glycosides are hydrolyzed into acids to separate free sugars and aglycones. Panaxadiol, panaxatriol and oleanolic acid are aglycones of ginseng ginsenosides.

On the other hand, red ginseng has been reported to produce new active ingredients unique to red ginseng or white ginseng, or increase the content of some active ingredients. However, red ginseng or black ginseng is manufactured by increasing the raw ginseng and drying it 1-9 times. Steam energy is input during steaming, and drying energy such as hot air drying, microwave drying, cold air drying and far-infrared drying methods in the drying process. Has been produced in a high energy consumption process (see FIG. 1).

In addition, there is an important problem that loss of commerciality by loss of merchandise, leakage of steaming liquid in the lower part of the steamer and the loss of quality due to the degradation of the quality caused by the phenomenon of back skin phenomenon and burst phenomenon in the steaming process.

The present invention is a method to solve the inefficiency and economic efficiency of the current red ginseng manufacturing process is a technology that can increase the total ginsenoside content contained in fresh ginseng 50-120% or more compared to red ginseng under high pressure conditions (See Table 9). Therefore, if the high-pressure treatment technology is applied to the extraction process of ginseng useful ingredients, it can be a hygienic economic process of low energy and high productivity that can improve the disadvantages of the current red ginseng manufacturing.

In this regard, most of the domestic and foreign patents and papers on food high pressure technology are pasteurization-oriented technologies. Looking at the technical contents, the pressure is 30-700 MPa and the high-pressure treatment time is 1-5 minutes. Doing.

 Attempts have been made to utilize high pressure techniques as extraction and separation techniques for the extraction and separation of plant bioactive components and to decompose plant cell walls, but the treatment time does not exceed 30 minutes due to the low pressure resistance of the high pressure system. . In order to increase the extraction rate and decomposition rate of vegetable components at low temperature, a pressure treatment of at least 4 hours is required, or a system capable of maintaining a pressure of 400 MPa or more up to 24 hours when inducing an enzymatic decomposition reaction is required. The results of this paper report high pressure technology that combines ultrasonic, electric field and high temperature treatment to make up for the shortcomings of the limited pressure resistance of pressure vessels. These technologies are considered to be uncompetitive in the era of global technological competition requiring low energy and low carbon production technology. do.

In the present invention, to extract useful components of ginseng using a low energy, to propose a more economic method.

Extraction method of the useful components of ginseng according to the present invention is as follows.

Conventional high pressure technology has been industrially used for the sterilization and sterilization of harmful microorganisms under ultrahigh pressure conditions in the range of 700-1000 MPa. In recent years, however, high pressure technology in the food field is one of the non-heating treatment methods beyond the scope of sterilization or sterilization in the food production process and low molecular dissolution of functional food raw materials without destroying or losing food nutrients and intrinsic ingredients at temperatures below 50 ℃. Techniques that can be utilized for the purpose of extracting or extracting are being attempted. In particular, biomolecules that have been made low-molecularly by increasing the solubility and extraction rate by high pressure treatment can maximize the digestive absorption rate of the human body and maintain the color, flavor, and nutritional value of useful ingredients. Compared with the product, it is possible to produce a product with different functional characteristics. Therefore, when decomposing by applying an enzyme suitable for the decomposition of the raw material at a high pressure of 50-500 MPa and a temperature of 50 ℃ can improve the productivity and efficiency in producing high quality extraction and decomposition products compared to the conventional heat treatment process. have.

      At 300 MPa under high pressure, protease trypsin showed 40% more activity than normal pressure, but thermolysin showed less than 5% residual activity, so the pressure resistance of enzyme was closely related to the mechanism of action. In other words, comparing the structure of the active site of trypsin and thermolysin enzyme, the active site of trypsin, a serine protease, does not destroy under high pressure conditions because only unique bonds exist, whereas thermolysin, a type of metalloprotease, is a zinc (Zn) ion. Coordination bonds not only bind the active sites amino acids histidine and glutamic acid, but also play an important role in catalysis of enzymes. Therefore, high-pressure treatment destroys the coordination bonds by zinc, and as a result, thermolysin has been reported to lose enzymatic activity (Kim Nam-su et al., 2010, Korea Food Research Institute).

The ratio of fresh ginseng and water is 20 to 30% by weight of the ratio of fresh ginseng to water. Such numerical limitation is because the extraction ratio of water-soluble solids and water-soluble total saponin was the highest in this range, as confirmed in the following examples. The same shall apply hereinafter.

Enzyme concentration is used at 0.5% to 1.2% by weight based on fresh ginseng. This weight percentage is relative to the weight for fresh ginseng. The enzyme uses a complex enzyme consisting of 0.1% to 3% by weight of barley malt oil, starch, cellulose, and 0.3% to 3% by weight of carbohydrate hydrolase, based on fresh ginseng used as a whole raw material. Current commercially available such enzymes include Thermamyl, Viscozyme, and Cellulast. The enzyme is used to be 0.5 to 1.2% by weight relative to ginseng as described above. The enzyme is based on barley malt and may use one or more of the other three enzymes, preferably all three enzymes. The reason for limiting to the above-mentioned complex enzyme is that it is currently used as a commercial enzyme for food, and since these four enzymes showed a decomposition rate of more than 98%, it was judged that there was no need to add enzyme in consideration of economic efficiency. . .

When the water, fresh ginseng, and the enzyme are put into a high pressure reactor and pressure is applied, the pressure is adjusted to 100 MPa to 200 MPa.

The treatment time of the pressure is 24 hours to 48 hours.

The high pressure enzyme decomposition temperature is 55 ℃ to 60 ℃.

Among the above process conditions, the most economically extractable useful ingredients of ginseng are 0.6% enzyme, 100 MPa pressure, 24 hours, and 55 ° C high pressure conditions, as confirmed in the following examples.

The high pressure enzymatic decomposition technique proposed by the present invention can extract useful components of ginseng at the highest extraction rate using low energy, thereby providing a ginseng beverage using the same. In addition, the high-pressure enzyme decomposition technology according to the present invention is compared with the conventional red ginseng manufacturing process, as shown in Figure 1 can be manufactured at a low cost of 1/10 of the manufacturing cost is judged to be excellent economic effect.

Figure 1 shows the current red ginseng manufacturing process and compares the required cost in this method and the process according to the present invention.

Hereinafter, the present invention will be described in detail so that the present invention can be easily realized with ordinary knowledge in the art. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

The ginseng used in the present invention was used ginseng (4, 5, 6 years old) cultivated in 2009 Geumsan region Chungnam, red ginseng was prepared by using some of the ginseng under the same conditions as the current manufacturing method. The camphor used ginseng (3, 5, 7 years old) grown in Geumsan, Chungnam province. Table 2 shows the results of the general component analysis of ginseng raw materials used in the present invention.

In the following examples, the description was made mainly on fresh ginseng, but according to the present inventors, similar results were obtained in red ginseng, camphor ginseng, and wild ginseng.

General Ingredients of Ginseng Raw Materials Ginseng Sample moisture(%) Crude protein (%) Crude fat (%) Ashes (%) carbohydrate(%) Fresh ginseng 4 years old 77.1 4.2 0.28 1.4 17.02 Five years 76.7 3.8 0.27 1.2 18.03 Six years 74.3 4.2 0.26 1.3 19.94 Red ginseng 4 years old 15.3 15.6 0.53 4.4 64.17 Five years 15.8 15.1 0.71 4.8 63.59 Six years 17.1 14.4 0.54 3.9 64.06 Camphor 3-year-old 80.0 4.0 0.38 1.2 14.42 Five years 78.5 4.3 0.44 1.2 15.56 7 years old 76.6 3.7 0.46 1.1 18.14

[Example 1]: Method for Treating Autoclave According to the Present Invention

Enzyme source used to decompose the substrate in the high-pressure conditions, the ginseng is barley malt for starch degradation (commercial product, cultivating NACF) and Thermamyl 120L was used (Novo Nordisk, Denmark), cellulose, hemicellulose, xylan and glucan as Viscozyme for decomposition L (Novo Nordisk, Denmark) was used and Cellulast 1.5L (Novo Nordisk, Denmark) was used for cellulose and xylan digestion. Comparative group for the production of ginseng degradation product was the extract extracted three times at 100 ℃ ginseng and red ginseng. High-pressure enzymatic digestion of ginseng using the complex enzyme applied to the present invention was performed by adding 0.2% by weight of barley malt oil, 0.1% by weight of thermamyl, 0.2% by weight of Viscozyme, and 0.1% by weight of Cellulast, and adjusting the pH to 7.0. (25, 50, 75, 100, 125, 150, 175, 200 MPa), reaction time (4, 8, 12, 24, 32, 48 hours) and reaction temperature (40, 45, 50, 55, 60 ℃) The decomposition was carried out according to, and the hydrolysis solution and the residue were obtained through centrifugation (11,000 g, 10 minutes, 4 ° C.) after the completion of the reaction. After decomposition for 24 hours at a pressure of 100MPa and a temperature of 50 ℃, the enzyme was inactivated (95 ℃, 10 minutes) and the supernatant was separated to make a ginseng extract. As can be seen from the following examples, considering economics, the optimum conditions were 25% of ginseng to water ratio, 100 MPa, 55 ° C., and 24 hours at an enzyme addition amount of 0.6%. In the following Tables 3 to 8,% means weight%.

Changes in Water Soluble Solids and Saponin Contents of Fresh Ginseng and Water Fresh ginseng ratio to water (%) Water Soluble Solids (%) Water Soluble Total Saponin (%) 5 44.4. 2.4 10 56.7 3.1 15 67.3 3.6 20 85.1 4.7 25 98.7 5.2 30 81.8 4.5

* Pressure: 100 MPa, Pressure treatment time: 24 hours, Temperature: 55 ℃, Complex enzyme concentration 0.6%

Changes in Water Soluble Solids and Saponin Contents by Enzyme Concentration Enzyme Concentration (%) Water Soluble Solids (%) Water Soluble Total Saponin (%) 0.1 34.6 1.4 0.2 46.7 2.1 0.3 57.4 2.6 0.4 65.6 3.7 0.5 78.4 5.2 0.6 98.8 5.2 0.7 98.6 5.3 0.8 98.7 5.3 1.0 98.7 5.4 1.2 99.1 5.5

* Pressure: 100 MPa, Pressure treatment time: 24 hours, Temperature: 55 ℃, Ginseng ratio to water: 25%

Changes in Water Soluble Solids and Saponin Contents with Pressure Change Pressure (MPa) Water Soluble Solids (%) Water Soluble Total Saponin (%) 25 16.5 1.8 50 22.6 2.6 75 57.8 3.2 100 98.5 5.3 125 98.3 5.3 150 98.1 5.2 175 99.5 5.3 200 99.7 5.2

* Ratio of fresh ginseng and water: 25%, pressure treatment time: 24 hours, temperature: 55 ℃, complex enzyme concentration 0.6%

Changes in Water Soluble Solids and Saponin Contents with Pressure Treatment Time Pressure treatment (time) Water Soluble Solids (%) Water Soluble Total Saponin (%) 4 11.2 1.2 8 45.6 2.4 12 68.7 3.6 24 98.6 5.4 32 98.4 5.4 48 98.5 5.3

* Pressure: Fresh ginseng and water: 25%, Pressure: 100 MPa, Temperature: 55 ℃, Complex enzyme concentration 0.6%

Changes in Water Soluble Solids and Saponin Contents with Pressure Treatment Temperature Pressure Treatment Temperature (℃) Water Soluble Solids (%) Water Soluble Total Saponin (%) 40 57.5 1.4 45 65.3 2.7 50 88.4 3.8 55 98.4 5.4 60 98.6 5.4

* Ratio of fresh ginseng and water: 25%, pressure: 100 MPa, pressure treatment time: 24 hours, complex enzyme concentration 0.6%

The solid content, water soluble saponin, and extraction yield of high-density enzyme decomposition of fresh ginseng, red ginseng, and camphor ginseng are shown in [Table 8]. In the case of fresh ginseng, the water-soluble solids content was increased by about 2.1-2.4 times regardless of the growing period, compared to the control hot water extract after autoclaving according to the present invention. The extraction yield also increased 1.9-2.2 times compared to the control hot water extract after high pressure enzyme digestion, and the water soluble total saponin content also increased by 1.6 times, indicating that the high pressure enzyme digestion was more effective than the hot water extract. On the other hand, the water-soluble total saponin content tends to increase in proportion to the number of years of growing ginseng, it is expected that the total saponin content increases with longer growing period. In the case of red ginseng, water soluble solids increased 1.5-1.9 times, extraction yield 1.6-2.1 times, and water-soluble total saponin contents increased 1.2-1.7 times compared to control hot water extract after autoclaving. Seemed. On the other hand, camphor ginseng showed higher soluble solid content and extraction yield compared to hot water extraction after decomposing high-pressure enzyme than fresh ginseng and red ginseng, resulting in 2.6-3.0 and 2.4-3.1 times increase respectively. In particular, the water-soluble total saponin content of camphor ginseng was 1.6-1.7 times higher than that of hot water extract after high-pressure enzymatic digestion.

Ginseng, red ginseng, camphor ginseng Water Soluble Solids, Irradiation, and Yield after Treatment Ginseng Sample Extraction and Decomposition Method receptivity
Solid content (%) receptivity
Total Saponin (%) yield
(%) Fresh ginseng 4 years 44.98 2.70 50.72 5 years Hot water extraction 41.11 2.99 45.50 6 years 41.61 3.16 46.36 4 years 96.48 4.32 99.54 5 years High pressure enzyme 99.27 5.19 99.26 6 years 92.74 5.07 96.33 Red ginseng
4 years 46.60 3.04 49.86 5 years Hot water extraction 41.56 3.42 45.54 6 years 50.23 4.30 54.23 4 years 81.78 5.29 97.81 5 years High pressure enzyme 80.62 4.92 98.22 6 years 75.87 5.39 88.49 Camphor 3 years 37.38 4.31 40.65 6 years Hot water extraction 29.77 4.35 34.97 7 years 28.57 4.52 31.84 3 years 97.41 6.74 99.38 6 years High pressure enzyme 89.00 7.35 99.13 7 years 81.36 7.39 98.87

The ginsenoside contents after autoclaving of fresh ginseng, red ginseng and camphor ginseng are shown in [Table 9]. Comparing the total ginsenoside content of hot ginseng extracts after hydrolysis of fresh ginseng showed 17.6, 46.9, and 13.7% increase in 4, 5, and 6 years old roots, respectively. The total ginsenoside content was increased to 10.8 and 2.8%. In case of camphor ginseng, total ginsenoside contents were increased by 25.3, 21, and 14.7% in 4, 5 and 6 years, respectively, compared to hot water extraction after autolysis. The total ginsenoside content increased to 46.9% in 5 years of fresh ginseng, which contained more than 99.3% compared to 15.38% of red ginseng raw materials. It can be seen as an advantage to prepare a ginseng decomposition product having the same ginsenoside content. That is, referring to [Table 9], the total ginsenoside extraction rate is higher than that of hot water extraction, which is 46.9% when compared to 9.52% of hot water extraction compared to 13.99% of high pressure enzyme digestion. The results show high ginsenoside content. Therefore, the production of ginseng decomposed by high-pressure enzymatic decomposition proposed in the present invention is an economical green technology that provides productivity and reduction of manufacturing cost in consideration of thermal energy and manufacturing time input to the manufacturing process of red ginseng, which has the potential for industrial application. It is judged that it is technology.

Ginsenoside Contents of Autoclaved Ginseng sample Ginsenoside (mg / g) Recovery rate (%) Rg ₁ Re Rf Rg ₂ + Rh ₁ Rb ₁ Rc Rb ₂ Rb ₃ Rd Rg ₃ (S, R) Rh ₂ Total Fresh ginseng 4 years Raw material 2.06 2.58 0.63 0.37 2.25 1.47 0.82 0.16 0.28 0.01 0.00 10.62 - Hot water extraction 1.60 1.75 0.36 0.28 1.76 1.38 0.64 0.12 0.16 0.00 0.00 8.05 75.8 High pressure enzyme 2.44 2.58 0.49 0.33 2.11 0.90 1.11 0.21 1.21 0.00 0.00 11.38 107.2 5 years Raw material 2.67 2.34 0.64 0.47 2.48 1.49 0.72 0.13 0.23 0.00 0.00 11.17 - Hot water extraction 1.82 1.84 0.39 0.36 2.51 1.54 0.75 0.11 0.17 0.03 0.00 9.52 85.2 High pressure enzyme 3.16 2.80 0.58 0.46 2.87 0.90 1.33 0.24 1.63 0.03 0.00 13.99 125.2 6 years Raw material 2.48 2.40 0.66 0.18 2.55 1.23 1.03 0.20 0.27 0.00 0.00 11.00 - Hot water extraction 1.77 1.51 0.40 0.21 1.89 0.98 0.63 0.13 0.13 0.03 0.00 7.67 69.7 High pressure enzyme 2.16 1.83 0.39 0.21 1.44 0.43 0.68 0.20 0.71 0.02 0.00 8.06 73.2 Red ginseng 4 years Raw material 2.29 2.01 0.61 0.62 4.31 3.11 1.44 0.26 0.64 0.21 0.00 15.49 - Hot water extraction 1.41 1.44 0.33 0.39 2.51 1.79 0.91 0.18 0.26 0.16 0.00 9.38 60.5 High pressure enzyme 2.35 2.32 0.59 0.54 3.79 1.96 1.64 0.32 1.06 0.03 0.00 14.59 94.2 5 years Raw material 2.62 2.21 0.72 0.53 4.90 2.59 1.13 0.22 0.39 0.08 0.00 15.38 - Hot water extraction 1.68 1.68 0.43 0.40 3.14 1.88 1.08 0.21 0.30 0.15 0.00 10.95 71.2 High pressure enzyme 2.46 1.97 0.53 0.41 3.43 1.80 1.40 0.26 0.66 0.02 0.00 12.95 84.2 6 years Raw material 2.57 1.89 0.60 0.44 3.90 2.04 1.15 0.23 0.36 0.07 0.00 13.26 - Hot water extraction 1.75 1.43 0.37 0.40 3.47 1.90 1.14 0.22 0.33 0.20 0.00 11.23 84.7 High pressure enzyme 1.98 1.71 0.35 0.28 3.59 1.38 1.32 0.23 0.66 0.04 0.00 11.55 87.1 Camphor 3 years Raw material 1.56 6.46 0.78 1.10 3.97 3.34 1.41 0.22 0.75 0.00 0.00 19.59 - Hot water extraction 1.39 3.80 0.40 0.78 1.46 0.64 1.31 0.14 2.20 0.30 0.00 12.43 63.5 High pressure enzyme 1.01 3.91 0.57 1.00 3.62 2.63 1.20 0.21 0.61 0.82 0.00 15.57 79.5 6 years Raw material 3.62 7.05 1.48 1.38 7.74 5.05 2.38 0.49 0.81 0.01 0.00 30.01 - Hot water extraction 2.31 3.99 0.66 1.10 3.13 1.50 2.40 0.30 3.47 0.36 0.00 19.23 64.1 High pressure enzyme 2.19 4.07 0.90 1.11 6.71 3.63 2.32 0.41 0.78 1.14 0.00 23.27 77.5 7 years Raw material 7.14 7.02 1.76 1.67 8.71 3.64 1.47 0.34 0.60 0.03 0.00 32.37 - Hot water extraction 2.57 2.66 0.80 0.65 4.81 1.99 0.93 0.19 0.33 1.25 0.00 16.18 50.0 High pressure enzyme 3.10 3.06 0.72 0.95 3.40 1.37 1.97 0.26 3.21 0.52 0.00 18.56 57.3

As a result of analyzing the molecular structure of ginseng digestion to infer the change of ginsenoside content after autolysis of fresh ginseng, it was confirmed that a new molecular structure was formed after high pressure enzymatic digestion. The expected compounds are as follows.

1) Molecular Formula C ₁₂ H ₂₄ O ₁₂

2) Molecular formula C ₁₁ H ₂₀ O ₁₀

  ABC)

3) Molecular formula C ₆ H ₁₂ O ₆

  ABC)

4) Molecular Formula C ₄₂ H ₅₈ O ₁₅

  Ghana)

5) Molecular formula C ₄₂ H ₅₈ O ₁₅

  Ghana)

Claims (4)

Adding 20 parts by weight to 30 parts by weight of fresh ginseng based on 100 parts by weight of water and 100 parts by weight of water to the high pressure reactor;
Adding an enzyme to the autoclave; And
And extracting useful ingredients of ginseng for 24 hours to 48 hours by adjusting the pressure of the high pressure reactor to 55 ° C. to 60 ° C., and adjusting the pressure to 100 MPa to 200 MPa.
High-pressure enzyme, characterized in that the enzyme consists of 0.1 to 3 parts by weight of barley syrup oil extract, 0.1 to 3 parts by weight of teramyl, 0.1 to 3 parts by weight of biskozyme and 0.1 to 3 parts by weight of cellulose cellulose based on 100 parts by weight of the fresh ginseng. Extraction method of useful components of ginseng by decomposition.
delete delete The method of claim 1,
The total content of the enzyme is 0.6 parts by weight based on 100 parts by weight of the fresh ginseng,
Method for extracting useful components of ginseng by high pressure enzyme decomposition, characterized in that the high pressure reactor performs a high pressure enzyme decomposition reaction for 24 hours at 55 ℃ temperature conditions and pressure of 100MPa.

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