CN107019227A - A kind of Banana Peel Dietary Fiber, low sugar yoghurt and preparation method thereof - Google Patents
A kind of Banana Peel Dietary Fiber, low sugar yoghurt and preparation method thereof Download PDFInfo
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- CN107019227A CN107019227A CN201710288787.XA CN201710288787A CN107019227A CN 107019227 A CN107019227 A CN 107019227A CN 201710288787 A CN201710288787 A CN 201710288787A CN 107019227 A CN107019227 A CN 107019227A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/133—Fruit or vegetables
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Dairy Products (AREA)
Abstract
本发明涉及一种香蕉皮膳食纤维、低糖酸奶及其制备方法,属于食品加工技术领域。本发明的香蕉皮膳食纤维将废弃的香蕉皮制备成香蕉皮粉,加入护色剂维生素C,然后分别加入淀粉酶和胰蛋白酶进行两步酶解,得到香蕉皮膳食纤维。本发明加入维生素C可用于防止香蕉皮氧化变黑,使用淀粉酶和胰蛋白酶进行酶解,工艺路线简单,操作条件温和,提取率较高,并且制备得到的香蕉皮膳食纤维中蛋白质、淀粉去除更为彻底,产品纯度更高。本发明的低糖酸奶添加香蕉皮膳食纤维和低聚木糖、木糖醇,在保留酸奶营养的同时,既降低了酸奶中的含糖量,又弥补了酸奶膳食纤维不足的缺陷,满足了特殊人群的需求。
The invention relates to banana peel dietary fiber, low-sugar yogurt and a preparation method thereof, belonging to the technical field of food processing. The banana peel dietary fiber of the present invention prepares discarded banana peels into banana peel powder, adds color-protecting agent vitamin C, and then respectively adds amylase and trypsin to carry out two-step enzymolysis to obtain the banana peel dietary fiber. Adding vitamin C in the present invention can be used to prevent banana peels from oxidizing and blackening. Amylase and trypsin are used for enzymolysis, the process route is simple, the operating conditions are mild, the extraction rate is high, and the protein and starch in the prepared banana peel dietary fiber are removed. More thorough, higher product purity. The low-sugar yoghurt of the present invention is added with banana peel dietary fiber, xylooligosaccharides and xylitol. While retaining the nutrition of yoghurt, it not only reduces the sugar content in yoghurt, but also makes up for the deficiency of yoghurt dietary fiber, and satisfies special requirements. the needs of the crowd.
Description
技术领域technical field
本发明涉及一种香蕉皮膳食纤维、低糖酸奶及其制备方法,属于食品加工技术领域。The invention relates to banana peel dietary fiber, low-sugar yogurt and a preparation method thereof, belonging to the technical field of food processing.
背景技术Background technique
酸奶是一种以新鲜的牛乳或复原乳为原料,经巴氏杀菌后,接种有益菌(发酵剂),然后发酵、冷藏后熟所得到的一种乳制品,其组织状态良好,凝结细腻,口味酸甜可口,深受消费者喜爱。经发酵后,牛乳中的糖、蛋白质、脂类等被分解成一些小分子(如葡萄糖、半乳糖、短键肽、游离氨基酸以及低级脂肪酸等),同时还会产生一些生理活性物质(如有机酸、芳香物质、抗生物质、细胞壁外多糖、活性酸、乳酸菌增值因子以及乳酸菌等)。与牛乳相比,经发酵的酸奶的营养价值更趋于完善,也更利于人体的消化吸收,对平衡人体肠道菌群活力,提高人体免疫力,改善部分人群的乳糖不耐受症均有一定的功效。Yogurt is a kind of dairy product obtained by using fresh milk or reconstituted milk as raw material, pasteurized, inoculated with beneficial bacteria (starter), then fermented, refrigerated and cooked. The taste is sweet and sour, and is deeply loved by consumers. After fermentation, sugars, proteins, lipids, etc. in milk are decomposed into small molecules (such as glucose, galactose, short-key peptides, free amino acids, and low-level fatty acids, etc.), and some physiologically active substances (such as organic Acids, aromatic substances, antibiotics, extracellular polysaccharides, active acids, lactic acid bacteria value-added factors and lactic acid bacteria, etc.). Compared with milk, the nutritional value of fermented yogurt tends to be more perfect, and it is also more conducive to the digestion and absorption of the human body. It can balance the vitality of human intestinal flora, improve human immunity, and improve the lactose intolerance of some people. Certain effect.
乳在乳酸菌的作用下会产生大量的酸味物质,使酸奶的风味变得尖锐过酸,为了改善口感,往往要添加甜味剂,以形成酸奶酸甜可口的口味。蔗糖是常用的天然营养型甜味剂,但服用大量的蔗糖容易引起人体血糖升高。Under the action of lactic acid bacteria, milk will produce a lot of sour substances, making the flavor of yogurt sharp and too sour. In order to improve the taste, sweeteners are often added to form yogurt with a sweet and sour taste. Sucrose is a commonly used natural nutritional sweetener, but taking a large amount of sucrose can easily cause blood sugar to rise.
膳食纤维是人体必不可少的营养元素,被称为是继蛋白质、脂肪、糖类、维生素、矿物质和水之后的第七大营养素。它是一种极其重要的功能性食品原料,不仅具有良好的持水性、吸附性,还能有效改善食品的色泽及风味。人体摄取足够的膳食纤维能改善肠道菌群,预防肥胖、心血管疾病、糖尿病、癌症等疾病。Dietary fiber is an essential nutrient element for the human body, known as the seventh largest nutrient after protein, fat, sugar, vitamins, minerals and water. It is an extremely important functional food raw material. It not only has good water holding capacity and adsorption property, but also can effectively improve the color and flavor of food. Sufficient dietary fiber intake by the human body can improve intestinal flora and prevent obesity, cardiovascular disease, diabetes, cancer and other diseases.
近年来,将膳食纤维应用于乳制品中已有报道。例如,2010年8月18日公开的CN101803634A中国发明专利申请公开说明书公开的“一种海带膳食纤维酸奶及其生产方法”是由鲜牛奶、海带膳食纤维、稳定剂、蔗糖、酸奶发酵剂组成,其具有防治便秘,降低胆固醇,调节血脂、血糖,特别适于中老年人、糖尿病病人和肥胖者食用。2012年1月11日公开的CN102308876A中国发明专利申请公开说明书公开的“花生渣纳米膳食纤维制作酸奶的方法”采用化学-酶法结合提取膳食纤维,并将其添加到奶液中混合发酵制作酸奶。2013年4月3日公开的CN103004981A中国发明专利申请公开说明书公开的“一种富含膳食纤维的酸奶”由脱脂奶粉、玉米膳食纤维、糖、酸奶发酵剂、稳定剂等组成。2015年12月30日公开的CN105192062A中国发明专利公开说明书公开的“一种香蕉酸奶”是一款由香蕉汁、甜味剂、低脂奶粉、膳食纤维、低聚木糖等混合液发酵而成的一种具有香蕉风味的酸奶制品。2016年5月25日公开的CN105594856A中国发明专利申请公开说明书公开的“一种高膳食纤维酸奶及生产方法”是由乳、低聚果糖、乳清蛋白、蔗糖、抗性糊精、酸奶发酵剂等组成。其中的抗性糊精是一种可溶性膳食纤维,该发明可以复合乳酸菌及膳食纤维的特性,均衡人体的膳食纤维摄入。香蕉皮中的膳食纤维含量较多,有助于人体的消化吸收,但香蕉皮一般被作为废弃物丢弃,造成了资源的浪费。另外目前还未有将低糖和香蕉皮膳食纤维与牛奶复合发酵制备香蕉皮膳食纤维低糖酸奶。In recent years, the application of dietary fiber in dairy products has been reported. For example, the CN101803634A Chinese invention patent application published on August 18, 2010 discloses "a kelp dietary fiber yogurt and its production method" which is composed of fresh milk, kelp dietary fiber, stabilizer, sucrose, and yogurt starter. It has the functions of preventing and treating constipation, lowering cholesterol, regulating blood fat and blood sugar, and is especially suitable for middle-aged and elderly people, diabetic patients and obese people. The CN102308876A Chinese invention patent application published on January 11, 2012 discloses the "method for making yoghurt with peanut dregs nano-dietary fiber", which uses a combination of chemical-enzyme method to extract dietary fiber, and adds it to milk liquid for mixed fermentation to make yoghurt . CN103004981A Chinese invention patent application published on April 3, 2013 discloses "a kind of yoghurt rich in dietary fiber" which consists of skimmed milk powder, corn dietary fiber, sugar, yoghurt starter, stabilizer and the like. The "banana yoghurt" disclosed in CN105192062A Chinese Invention Patent Publication published on December 30, 2015 is fermented from a mixture of banana juice, sweetener, low-fat milk powder, dietary fiber, and xylo-oligosaccharides. A banana-flavored yogurt product. "A high dietary fiber yoghurt and its production method" disclosed in CN105594856A Chinese invention patent application published on May 25, 2016 is composed of milk, fructooligosaccharides, whey protein, sucrose, resistant dextrin, yoghurt starter and so on. The resistant dextrin is a kind of soluble dietary fiber. The invention can combine the characteristics of lactic acid bacteria and dietary fiber to balance the dietary fiber intake of the human body. The dietary fiber content in the banana peel is more, which is helpful for the digestion and absorption of the human body, but the banana peel is generally discarded as waste, resulting in a waste of resources. In addition, there is no low-sugar yogurt prepared by low-sugar and banana peel dietary fiber and milk compound fermentation at present.
发明内容Contents of the invention
本发明的目的在于提供一种香蕉皮膳食纤维,利用废弃的香蕉皮制备成适于食用的纤维食品。The object of the present invention is to provide a kind of dietary fiber of banana peel, utilize discarded banana peel to prepare the fiber food that is suitable for eating.
本发明的另一个目的在于提供一种添加上述香蕉皮膳食纤维的低糖酸奶,该酸奶既降低了含糖量,同时又提高了膳食纤维的含量。Another object of the present invention is to provide a low-sugar yoghurt added with the above-mentioned banana peel dietary fiber, which reduces the sugar content and increases the dietary fiber content.
本发明还提供了上述低糖酸奶的制备方法。The present invention also provides a preparation method of the above-mentioned low-sugar yoghurt.
为了实现上述目的,本发明所采用的技术方案是:In order to achieve the above object, the technical solution adopted in the present invention is:
一种香蕉皮膳食纤维,由包括如下步骤的方法制得:A kind of banana peel dietary fiber is made by the method comprising the following steps:
1)取香蕉皮粉与水混合,得粉浆;1) Take banana peel powder and mix with water to obtain powder slurry;
2)将粉浆与维生素C混合,煮沸灭酶;调节体系pH至5-7,加入淀粉酶进行酶解;然后调节体系pH至7-8,加入胰蛋白酶进行酶解,得酶解液;2) Mix the powder slurry with vitamin C, boil to inactivate the enzyme; adjust the pH of the system to 5-7, add amylase for enzymolysis; then adjust the pH of the system to 7-8, add trypsin for enzymolysis to obtain an enzymolysis solution;
3)将酶解液固液分离,将分离得到的液体浓缩得浓缩液,将浓缩液醇析、过滤得滤饼,将滤饼干燥即得香蕉皮膳食纤维。3) Separating the enzymolysis liquid from solid to liquid, concentrating the separated liquid to obtain a concentrate, ethanolizing the concentrate, filtering to obtain a filter cake, drying the filter cake to obtain the banana peel dietary fiber.
所述香蕉皮粉为将香蕉皮洗净、干燥、粉碎后得到。The banana peel powder is obtained by washing, drying and pulverizing the banana peel.
优选的,步骤1)中取香蕉皮粉与水以1:20-50的质量比混合,得粉浆。Preferably, in step 1), banana peel powder is mixed with water at a mass ratio of 1:20-50 to obtain a slurry.
步骤2)中,维生素C的用量为:维生素C的质量为香蕉皮粉质量的0.5-1.5%。In step 2), the dosage of vitamin C is: the quality of vitamin C is 0.5-1.5% of the quality of banana peel powder.
步骤2)中煮沸2-3min灭酶。In step 2), boil for 2-3min to inactivate the enzyme.
步骤2)中,淀粉酶的用量为:淀粉酶的质量为香蕉皮粉质量的0.6-0.8%。In step 2), the consumption of amylase is: the quality of amylase is 0.6-0.8% of the quality of banana peel powder.
步骤2)中加入淀粉酶于75-85℃酶解45-75min。In step 2), amylase is added to enzymatically hydrolyze at 75-85°C for 45-75min.
步骤2)中,胰蛋白酶的用量为:胰蛋白酶的质量为香蕉皮粉质量的0.3-0.6%。In step 2), the dosage of trypsin is: the quality of trypsin is 0.3-0.6% of the quality of banana peel powder.
步骤2)中加入胰蛋白酶于45-55℃酶解85-115min。In step 2), trypsin was added to enzymatically digest at 45-55° C. for 85-115 minutes.
所述淀粉酶的酶活力≥3700u/g。The enzyme activity of the amylase is more than or equal to 3700u/g.
所述胰蛋白酶的酶活力≥2500u/g。The enzyme activity of the trypsin is more than or equal to 2500u/g.
步骤3)中所述醇析为将浓缩液与3.5-4.5倍体积的无水乙醇混合。The alcohol analysis in step 3) is to mix the concentrate with 3.5-4.5 times volume of absolute ethanol.
步骤3)中所述醇析时间为2-3h。The ethanol analysis time described in step 3) is 2-3h.
步骤3)中所述浓缩为浓缩至分离到的液体原体积的1/3-1/4。The concentration described in step 3) is concentrated to 1/3-1/4 of the original volume of the separated liquid.
步骤3)中所述浓缩为旋转蒸发浓缩,旋转蒸发参数为:温度55-65℃,转速40-60rmp。The concentration in step 3) is concentrated by rotary evaporation, and the parameters of rotary evaporation are: temperature 55-65°C, rotation speed 40-60rmp.
优选的,步骤3)中将滤饼干燥后,超微粉碎,然后过150-250目筛,即得香蕉皮膳食纤维。Preferably, after the filter cake is dried in step 3), it is superfinely pulverized, and then passed through a 150-250 mesh sieve to obtain the banana peel dietary fiber.
本发明的香蕉皮膳食纤维,将废弃的香蕉皮制备成香蕉皮粉,加入维生素C,然后进行两步酶解,得到香蕉皮膳食纤维。本发明加入维生素C可用于防止香蕉皮氧化变黑,使用淀粉酶和胰蛋白酶进行酶解,工艺路线简单,操作条件温和,提取率较高,并且制备得到的香蕉皮膳食纤维中蛋白质、淀粉去除更为彻底,产品纯度更高。The banana peel dietary fiber of the present invention prepares banana peel powder from discarded banana peel, adds vitamin C, and then performs two-step enzymatic hydrolysis to obtain the banana peel dietary fiber. Adding vitamin C in the present invention can be used to prevent the oxidation and blackening of banana peels. Amylase and trypsin are used for enzymolysis, the process route is simple, the operating conditions are mild, the extraction rate is high, and the protein and starch in the prepared banana peel dietary fiber are removed. More thorough, higher product purity.
一种采用上述的香蕉皮膳食纤维的低糖酸奶,由以下重量百分比的原料制成:复原乳75-85%,所述香蕉皮膳食纤维1-3%,糖液10-20%,菌种3-6%;所述糖液由质量分数为40-60%木糖醇、5-30%低聚木糖与30-40%水组成。A low-sugar yogurt using the above-mentioned banana peel dietary fiber, made of the following raw materials in weight percentage: 75-85% of reconstituted milk, 1-3% of the banana peel dietary fiber, 10-20% of sugar solution, and 3 strains -6%; the sugar solution is composed of 40-60% xylitol, 5-30% xylooligosaccharide and 30-40% water.
本发明的酸奶中的木糖醇是一种天然甜味剂,可以防止糖尿病人的血糖升高,消除糖尿病人三多,改善肝功能,保护牙齿的功效,是糖尿病人安全的甜味剂、营养补充剂和辅助治疗剂。本发明中的酸奶中的低聚木糖是一种功能性甜味剂,不易被人体水解、吸收,不影响人体的血糖浓度,在高温和酸性条件下,不易被分解,具有良好的酸、热稳定性。本发明的酸奶中的低聚木糖是一种良好的益生元,对有益菌增殖效果明显,同时具有减少有毒发酵产物及有害细菌酶产生,抑制病原菌和腹泻,防止便秘,保护肝脏功能,降低血清胆固醇和血压,抗癌的功能。人体摄入一定量的低聚木糖,还能促进钙的吸收,促使机体生成包括维生素B1、B2、B6、B12、烟酸和叶酸等在内的多种营养物质。The xylitol in the yoghurt of the present invention is a kind of natural sweetener, can prevent the blood sugar of diabetic patient from rising, eliminate three excesses of diabetic patient, improve liver function, the effect of protecting teeth, is the safe sweetener of diabetic patient, Nutritional supplements and adjuvant therapeutics. The xylooligosaccharide in the yogurt of the present invention is a functional sweetener, which is not easily hydrolyzed and absorbed by the human body, does not affect the blood sugar concentration of the human body, is not easily decomposed under high temperature and acidic conditions, and has good acid, thermal stability. The xylo-oligosaccharide in the yogurt of the present invention is a good prebiotic, which has obvious effect on the proliferation of beneficial bacteria, and at the same time can reduce the production of toxic fermentation products and harmful bacterial enzymes, inhibit pathogenic bacteria and diarrhea, prevent constipation, protect liver function, reduce Serum cholesterol and blood pressure, anticancer function. The intake of a certain amount of xylooligosaccharides by the human body can also promote the absorption of calcium and promote the body to produce various nutrients including vitamins B1, B2, B6, B12, niacin and folic acid.
所述复原乳由以下重量百分比的组分组成:10-20%的全脂乳粉,3-8%的脱脂乳粉,72-87%的水。The reconstituted milk is composed of the following components by weight percentage: 10-20% whole milk powder, 3-8% skimmed milk powder, and 72-87% water.
所述菌种为嗜热链球菌与保加利亚乳杆菌的混合菌种。The strain is a mixed strain of Streptococcus thermophilus and Lactobacillus bulgaricus.
所述菌种中活菌数应大于等于100万cfu/g。The number of viable bacteria in the strain should be greater than or equal to 1 million cfu/g.
优选的,所述嗜热链球菌与保加利亚乳杆菌的菌种质量比为(1-2):(1-2)。Preferably, the mass ratio of Streptococcus thermophilus to Lactobacillus bulgaricus is (1-2): (1-2).
优选的,所述嗜热链球菌与保加利亚乳杆菌的菌种质量比为(1-2):2。Preferably, the mass ratio of Streptococcus thermophilus to Lactobacillus bulgaricus is (1-2):2.
上述的低糖酸奶的制备方法,包括:取配方量的复原乳、香蕉皮膳食纤维与所述糖液混合,得混合液;将混合液灭菌,然后接入菌种于40-45℃下发酵3-5h,冷却后熟,即得。The preparation method of the above-mentioned low-sugar yoghurt comprises: mixing the reconstituted milk and the dietary fiber of banana peel with the sugar liquid in a formula amount to obtain a mixed liquid; 3-5h, cooked after cooling, that is to say.
优选的,取配方量的木糖醇、低聚木糖与水于50-60℃混合,得糖液。Preferably, xylitol, xylo-oligosaccharides and water are mixed at a temperature of 50-60° C. to obtain a sugar solution.
优选的,取配方量的复原乳、香蕉皮膳食纤维与所述糖液混合,于50-70℃、15-25MPa下均质处理,得混合液。Preferably, the formula amount of reconstituted milk and banana peel dietary fiber is mixed with the sugar liquid, and homogenized at 50-70° C. and 15-25 MPa to obtain a mixed liquid.
优选的,所述灭菌为将混合液于85-95℃灭菌10-20min。Preferably, the sterilization is to sterilize the mixture at 85-95° C. for 10-20 minutes.
优选的,将混合液灭菌后冷却至40-45℃,然后接入菌种。Preferably, the mixed solution is sterilized and cooled to 40-45° C., and then inoculated with strains.
所述冷却后熟为冷却至室温,然后于0-8℃放置12-24h。The cooling post-cooking is cooling to room temperature, and then standing at 0-8°C for 12-24h.
本发明的低糖酸奶的制备方法在复原乳中添加低聚木糖、木糖醇、香蕉皮膳食纤维,均质、灭菌、冷却后加入酸奶发酵剂发酵、冷却后熟而成的,生产工艺先进合理、操作性强,适用于工业化生产。The preparation method of low-sugar yogurt of the present invention is obtained by adding xylooligosaccharide, xylitol, and banana peel dietary fiber to reconstituted milk, homogenizing, sterilizing, cooling, adding yogurt starter to ferment, and cooling and ripening. The production process Advanced and reasonable, strong operability, suitable for industrial production.
附图说明Description of drawings
图1为实施例1中香蕉皮膳食纤维制备流程图;Fig. 1 is the flow chart of preparing banana peel dietary fiber in embodiment 1;
图2为实施例1中低糖酸奶制备流程图。Fig. 2 is the flow chart of the preparation of low-sugar yogurt in Example 1.
具体实施方式detailed description
下面结合具体实施例对本发明做进一步的详细说明。The present invention will be further described in detail below in conjunction with specific embodiments.
以下实施例中的淀粉酶的酶活力≥3700u/g,胰蛋白酶的酶活力≥2500u/g。The enzyme activity of amylase in the following examples is ≥3700u/g, and the enzyme activity of trypsin is ≥2500u/g.
实施例1Example 1
本实施例中香蕉皮膳食纤维由包括如下步骤的方法制得(制备流程如图1所示):Banana peel dietary fiber is made by the method comprising the following steps in the present embodiment (preparation process is as shown in Figure 1):
1)取新鲜健康的香蕉皮,经洗净、干燥、粉碎预处理后得香蕉皮粉,将香蕉皮粉与水以1:20的质量比混合,得粉浆;1) Take fresh and healthy banana peels, wash, dry, and pulverize them to obtain banana peel powder, mix the banana peel powder with water at a mass ratio of 1:20, and obtain a slurry;
2)将粉浆与香蕉皮粉原料质量1%的维生素C混合,煮沸2min灭酶;加入1mol/LNaOH溶液调节体系pH至6,然后每kg香蕉皮粉原料加入6g淀粉酶于80℃酶解60min;然后加入1mol/L NaOH溶液调节pH至7,然后每kg香蕉皮粉原料加入胰蛋白酶3g于55℃酶解100min,得酶解液;2) Mix the powder slurry with 1% vitamin C of banana peel powder raw material mass, boil for 2 minutes to inactivate the enzyme; add 1mol/L NaOH solution to adjust the pH of the system to 6, then add 6g of amylase per kg of banana peel powder raw material to enzymatically hydrolyze at 80°C 60 minutes; then add 1mol/L NaOH solution to adjust the pH to 7, then add trypsin 3g per kg of banana peel powder raw material and enzymolyze at 55°C for 100min to obtain the enzymolysis solution;
3)将酶解液离心分离后取上清液,将上清液置于旋转蒸发仪中浓缩至原体积的1/3,旋转蒸发参数为:温度60℃,转速60rmp;然后将浓缩液与4倍体积的无水乙醇混合醇析2h,得醇析物;3) After centrifuging the enzymolysis solution, take the supernatant, put the supernatant in a rotary evaporator and concentrate it to 1/3 of the original volume, the parameters of the rotary evaporation are: temperature 60°C, speed 60rmp; then the concentrated solution is mixed with 4 times the volume of absolute ethanol was mixed with ethanol for 2 hours to obtain the ethanol precipitate;
4)将醇析物过滤得滤饼,将滤饼干燥后,超微粉碎,然后过200目筛,即得香蕉皮膳食纤维。4) Filter the alcohol analyte to obtain a filter cake, dry the filter cake, ultrafinely pulverize it, and pass through a 200-mesh sieve to obtain the banana peel dietary fiber.
本实施例中的低糖酸奶由以下重量百分含量的原料制成:复原乳80%,香蕉皮膳食纤维1%,糖液15%,嗜热链球菌与保加利亚乳杆菌混合菌种4%;所述糖液由质量分数为40%木糖醇、30%低聚木糖与30%水组成。The low-sugar yogurt in this example is made from the following raw materials in percentage by weight: 80% of reconstituted milk, 1% of banana peel dietary fiber, 15% of sugar solution, 4% of mixed strains of Streptococcus thermophilus and Lactobacillus bulgaricus; Said sugar solution is composed of 40% xylitol, 30% xylooligosaccharide and 30% water in mass fraction.
所述嗜热链球菌与保加利亚乳杆菌的质量比为1:1。所述混合菌种中活菌数大于等于100万cfu/g。The mass ratio of Streptococcus thermophilus to Lactobacillus bulgaricus is 1:1. The number of viable bacteria in the mixed strain is greater than or equal to 1 million cfu/g.
所述复原乳由以下重量百分含量的组分组成:15%的全脂乳粉,5%的脱脂乳粉,80%的水。取配方量的全脂奶粉、脱脂奶粉加入配方量的水搅拌使其均匀溶解,即得复原乳。The reconstituted milk is composed of the following components in percentage by weight: 15% whole milk powder, 5% skimmed milk powder, and 80% water. Take the formula amount of whole milk powder and skim milk powder, add the formula amount of water and stir to dissolve evenly, and then obtain the reconstituted milk.
上述的低糖酸奶的制备方法(制备流程如图2所示),包括:取配方量的木糖醇、低聚木糖与水于55℃混合,得糖液;取配方量的复原乳、香蕉皮膳食纤维与所述糖液混合,于50℃、25MPa下均质处理,得混合液;将混合液于85℃灭菌20min;然后冷却至45℃,接入菌种于42℃下发酵3h;冷却至室温,然后于4℃放置12h,即得。The preparation method of the above-mentioned low-sugar yoghurt (the preparation process is shown in Figure 2), comprising: taking xylitol and xylo-oligosaccharides of formula quantity and mixing with water at 55°C to obtain sugar liquid; taking formula quantity of reconstituted milk, banana Mix the skin dietary fiber with the sugar solution, and homogenize it at 50°C and 25MPa to obtain a mixed solution; sterilize the mixed solution at 85°C for 20 minutes; then cool it to 45°C, insert bacteria and ferment at 42°C for 3 hours ; Cool to room temperature, and then placed at 4 ° C for 12h, that is, too.
实施例2Example 2
本实施例中香蕉皮膳食纤维由包括如下步骤的方法制得:Banana peel dietary fiber is made by the method comprising the following steps in the present embodiment:
1)取新鲜健康的香蕉皮,经洗净、干燥、粉碎预处理后得香蕉皮粉,将香蕉皮粉与水以1:30的质量比混合,得粉浆;1) Take fresh and healthy banana peels, wash, dry, and pulverize them to obtain banana peel powder, mix the banana peel powder with water at a mass ratio of 1:30, and obtain a slurry;
2)将粉浆与香蕉皮粉原料质量1%的维生素C混合,煮沸2min灭酶;加入1mol/LNaOH溶液调节体系pH至5,然后每kg香蕉皮粉原料加入6g淀粉酶于85℃酶解60min;然后加入1mol/L NaOH溶液调节pH至7,然后每kg香蕉皮粉原料加入胰蛋白酶3g于55℃酶解100min,得酶解液;2) Mix the powder slurry with 1% vitamin C of banana peel powder raw material mass, boil for 2 minutes to inactivate the enzyme; add 1mol/L NaOH solution to adjust the pH of the system to 5, then add 6g of amylase per kg of banana peel powder raw material to enzymatically hydrolyze at 85°C 60 minutes; then add 1mol/L NaOH solution to adjust the pH to 7, then add trypsin 3g per kg of banana peel powder raw material and enzymolyze at 55°C for 100min to obtain the enzymolysis solution;
3)将酶解液离心分离后取上清液,将上清液置于旋转蒸发仪中浓缩至原体积的1/3,旋转蒸发参数为:温度65℃,转速40rmp;然后将浓缩液与4倍体积的无水乙醇混合醇析2h,得醇析物;3) After centrifuging the enzymatic solution, take the supernatant, put the supernatant in a rotary evaporator and concentrate it to 1/3 of the original volume, the parameters of the rotary evaporation are: temperature 65°C, speed 40rmp; then the concentrated solution is mixed with 4 times the volume of absolute ethanol was mixed with ethanol for 2 hours to obtain the ethanol precipitate;
4)将醇析物过滤得滤饼,将滤饼干燥后,超微粉碎,然后过200目筛,即得香蕉皮膳食纤维。4) Filter the alcohol analyte to obtain a filter cake, dry the filter cake, ultrafinely pulverize it, and pass through a 200-mesh sieve to obtain the banana peel dietary fiber.
本实施例中的低糖酸奶由以下重量百分含量的原料制成:复原乳75%,香蕉皮膳食纤维2%,糖液20%,嗜热链球菌与保加利亚乳杆菌混合菌种3%;所述糖液由质量分数为40%木糖醇、20%低聚木糖与40%水组成。The low-sugar yogurt in this example is made from the following raw materials in percentage by weight: 75% reconstituted milk, 2% dietary fiber from banana peel, 20% sugar solution, 3% mixed strains of Streptococcus thermophilus and Lactobacillus bulgaricus; Said sugar solution is composed of 40% xylitol, 20% xylooligosaccharide and 40% water in mass fraction.
所述嗜热链球菌与保加利亚乳杆菌的质量比为1.5:1。所述混合菌种中活菌数大于等于100万cfu/g。The mass ratio of Streptococcus thermophilus to Lactobacillus bulgaricus is 1.5:1. The number of viable bacteria in the mixed strain is greater than or equal to 1 million cfu/g.
所述复原乳由以下重量百分含量的组分组成:15%的全脂乳粉,5%的脱脂乳粉,80%的水。取配方量的全脂奶粉、脱脂奶粉加入配方量的水搅拌使其均匀溶解,即得复原乳。The reconstituted milk is composed of the following components in percentage by weight: 15% whole milk powder, 5% skimmed milk powder, and 80% water. Take the formula amount of whole milk powder and skim milk powder, add the formula amount of water and stir to dissolve evenly, and then obtain the reconstituted milk.
上述的低糖酸奶的制备方法,包括如下步骤:取配方量的木糖醇、低聚木糖与水于55℃混合,得糖液;取配方量的复原乳、香蕉皮膳食纤维与所述糖液混合,于60℃、20MPa下均质处理,得混合液;将混合液于95℃灭菌15min;然后冷却至42℃,接入菌种于42℃下发酵4h;冷却至室温,然后于4℃放置24h,即得。The preparation method of the above-mentioned low-sugar yoghurt comprises the following steps: taking formula quantity of xylitol, xylo-oligosaccharide and mixing water at 55°C to obtain sugar solution; taking formula quantity of reconstituted milk, banana peel dietary fiber and said sugar mixed solution, and homogeneously treated at 60°C and 20MPa to obtain a mixed solution; the mixed solution was sterilized at 95°C for 15 minutes; then cooled to 42°C, and fermented at 42°C for 4 hours; cooled to room temperature, and then Place it at 4°C for 24h, and it is ready.
实施例3Example 3
本实施例中香蕉皮膳食纤维由包括如下步骤的方法制得:Banana peel dietary fiber is made by the method comprising the following steps in the present embodiment:
1)取新鲜健康的香蕉皮,经洗净、干燥、粉碎预处理后得香蕉皮粉,将香蕉皮粉与水以1:50的质量比混合,得粉浆;1) Take fresh and healthy banana peels, wash, dry, and pulverize them to obtain banana peel powder, mix the banana peel powder with water at a mass ratio of 1:50, and obtain a slurry;
2)将粉浆与香蕉皮粉原料质量1%的维生素C混合,煮沸2min灭酶;加入1mol/LNaOH调节体系pH至6,然后每kg香蕉皮粉原料加入8g淀粉酶于80℃酶解60min;然后加入1mol/L NaOH调节pH至7,然后每kg香蕉皮粉原料加入胰蛋白酶6g于55℃酶解100min,得酶解液;2) Mix the powder slurry with 1% vitamin C of banana peel powder raw material mass, boil for 2 minutes to inactivate the enzyme; add 1mol/L NaOH to adjust the pH of the system to 6, then add 8g of amylase per kg of banana peel powder raw material and enzymolyze at 80°C for 60 minutes ; Then add 1mol/L NaOH to adjust the pH to 7, then add trypsin 6g per kg of banana peel powder raw material and enzymolyze at 55°C for 100min to obtain an enzymolysis solution;
3)将酶解液离心分离后取上清液,将上清液置于旋转蒸发仪中浓缩至原体积的1/3,旋转蒸发参数为:温度60℃,转速60rmp;然后将浓缩液与4倍体积的无水乙醇混合醇析3h,得醇析物;3) After centrifuging the enzymolysis solution, take the supernatant, put the supernatant in a rotary evaporator and concentrate it to 1/3 of the original volume, the parameters of the rotary evaporation are: temperature 60°C, speed 60rmp; then the concentrated solution is mixed with 4 times the volume of absolute ethanol was mixed with ethanol for 3 hours to obtain the ethanol precipitate;
4)将醇析物过滤得滤饼,将滤饼干燥后,超微粉碎,然后过200目筛,即得香蕉皮膳食纤维。4) Filter the alcohol analyte to obtain a filter cake, dry the filter cake, ultrafinely pulverize it, and pass through a 200-mesh sieve to obtain the banana peel dietary fiber.
本实施例中的低糖酸奶由以下重量百分含量的原料制成:复原乳85%,香蕉皮膳食纤维1%,糖液10%,嗜热链球菌与保加利亚乳杆菌混合菌种4%;所述糖液由质量分数为60%木糖醇、10%低聚木糖与30%水组成。The low-sugar yogurt in this example is made of the following raw materials in percentage by weight: 85% reconstituted milk, 1% dietary fiber from banana peel, 10% sugar solution, 4% mixed strains of Streptococcus thermophilus and Lactobacillus bulgaricus; The sugar solution is composed of 60% xylitol, 10% xylooligosaccharide and 30% water in mass fraction.
所述嗜热链球菌与保加利亚乳杆菌的质量比为1:1.5。所述混合菌种中活菌数大于等于100万cfu/g。The mass ratio of Streptococcus thermophilus to Lactobacillus bulgaricus is 1:1.5. The number of viable bacteria in the mixed strain is greater than or equal to 1 million cfu/g.
所述复原乳由以下重量百分含量的组分组成:15%的全脂乳粉,5%的脱脂乳粉,80%的水。取配方量的全脂奶粉、脱脂奶粉加入配方量的水搅拌使其均匀溶解,即得复原乳。The reconstituted milk is composed of the following components in percentage by weight: 15% whole milk powder, 5% skimmed milk powder, and 80% water. Take the formula amount of whole milk powder and skim milk powder, add the formula amount of water and stir to dissolve evenly, and then obtain the reconstituted milk.
上述的低糖酸奶的制备方法,包括如下步骤:取配方量的木糖醇、低聚木糖与水于55℃混合,得糖液;取配方量的复原乳、香蕉皮膳食纤维、所述糖液混合,于50℃、25MPa下均质处理,得混合液;将混合液于95℃灭菌15min;然后冷却至42℃,接入菌种于42℃下发酵4h;冷却至室温,然后于4℃放置24h,即得。The preparation method of the above-mentioned low-sugar yogurt comprises the following steps: taking xylitol and xylo-oligosaccharides of formula quantity and mixing with water at 55° C. to obtain sugar solution; taking formula quantity of reconstituted milk, banana peel dietary fiber, said sugar mixed solution, and homogeneously treated at 50°C and 25MPa to obtain a mixed solution; sterilized the mixed solution at 95°C for 15 minutes; then cooled to 42°C, added strains and fermented at 42°C for 4 hours; cooled to room temperature, and then Place it at 4°C for 24h, and it is ready.
实施例4Example 4
本实施例中香蕉皮膳食纤维由包括如下步骤的方法制得:Banana peel dietary fiber is made by the method comprising the following steps in the present embodiment:
1)取新鲜健康的香蕉皮,经洗净、干燥、粉碎预处理后得香蕉皮粉,将香蕉皮粉与水以1:35的质量比混合,得粉浆;1) Take fresh and healthy banana peels, wash, dry, and pulverize them to obtain banana peel powder, mix the banana peel powder with water at a mass ratio of 1:35, and obtain a slurry;
2)将粉浆与香蕉皮粉原料质量0.5%质量的维生素C混合,煮沸灭酶3min;加入1mol/L NaOH溶液调节pH至6,然后每kg香蕉皮粉原料加入8g淀粉酶于80℃酶解45min;再次加入1mol/L NaOH溶液调节pH至8,然后每kg香蕉皮粉原料加入胰蛋白酶6g于55℃酶解85min,得酶解液;2) Mix the slurry with vitamin C with 0.5% mass of banana peel powder raw material, boil to inactivate the enzyme for 3 minutes; add 1mol/L NaOH solution to adjust the pH to 6, then add 8g of amylase per kg of banana peel powder raw material at 80°C to enzymatically Decompose for 45 minutes; add 1mol/L NaOH solution again to adjust the pH to 8, then add 6g of trypsin per kg of banana peel powder raw material and enzymolyze at 55°C for 85min to obtain an enzymatic hydrolysis solution;
3)将酶解液离心分离后取上清液,将上清液置于旋转蒸发仪中浓缩至原体积的1/4,旋转蒸发参数为:温度65℃,转速60rmp;然后将浓缩液与3.5倍的无水乙醇混合醇析3h,得醇析物;3) After centrifuging the enzymatic solution, take the supernatant, put the supernatant in a rotary evaporator and concentrate it to 1/4 of the original volume, the parameters of the rotary evaporation are: temperature 65°C, speed 60rmp; 3.5 times of absolute ethanol was mixed with ethanol for 3 hours to obtain the alcohol analyte;
4)将醇析物过滤得滤饼,将滤饼干燥后,超微粉碎,然后过250目筛,即得香蕉皮膳食纤维。4) Filter the alcohol analyte to obtain a filter cake, dry the filter cake, ultrafinely pulverize it, and pass through a 250-mesh sieve to obtain the banana peel dietary fiber.
本实施例中的低糖酸奶由以下重量百分含量的原料制成:复原乳80%,香蕉皮膳食纤维2%,糖液12%,嗜热链球菌与保加利亚乳杆菌混合菌种6%;所述糖液由质量分数为60%木糖醇、5%低聚木糖与35%水组成。The low-sugar yogurt in this example is made from the following raw materials in percentage by weight: 80% of reconstituted milk, 2% of dietary fiber from banana peel, 12% of sugar liquid, 6% of mixed strains of Streptococcus thermophilus and Lactobacillus bulgaricus; Said sugar solution is composed of 60% xylitol, 5% xylooligosaccharide and 35% water in mass fraction.
所述嗜热链球菌与保加利亚乳杆菌的质量比为1:1。所述混合菌种中活菌数大于等于100万cfu/g。The mass ratio of Streptococcus thermophilus to Lactobacillus bulgaricus is 1:1. The number of viable bacteria in the mixed strain is greater than or equal to 1 million cfu/g.
所述复原乳由以下重量百分含量的组分组成:10%的全脂乳粉,3%的脱脂乳粉,87%的水。取配方量的全脂奶粉、脱脂奶粉加入配方量的水搅拌使其均匀溶解,即得复原乳。The reconstituted milk is composed of the following components in percentage by weight: 10% whole milk powder, 3% skimmed milk powder, and 87% water. Take the formula amount of whole milk powder and skim milk powder, add the formula amount of water and stir to dissolve evenly, and then obtain the reconstituted milk.
上述的低糖酸奶的制备方法,包括如下步骤:取配方量的木糖醇、低聚木糖与水于60℃混合,得糖液;取配方量的复原乳、香蕉皮膳食纤维、所述糖液混合,于50℃、25MPa下均质处理,得混合液;将混合液于95℃灭菌15min;然后冷却至42℃,接入菌种于42℃下发酵4h;冷却至室温,然后于4℃放置24h,即得。The preparation method of the above-mentioned low-sugar yoghurt comprises the following steps: taking xylitol, xylo-oligosaccharides and water in a recipe amount and mixing them with water at 60°C to obtain a sugar liquid; taking a recipe amount of reconstituted milk, banana peel dietary fiber, the sugar mixed solution, and homogeneously treated at 50°C and 25MPa to obtain a mixed solution; sterilized the mixed solution at 95°C for 15 minutes; then cooled to 42°C, added strains and fermented at 42°C for 4 hours; cooled to room temperature, and then Place it at 4°C for 24h, and you can get it.
实施例5Example 5
本实施例中香蕉皮膳食纤维由包括如下步骤的方法制得:Banana peel dietary fiber is made by the method comprising the following steps in the present embodiment:
1)取新鲜健康的香蕉皮,经洗净、干燥、粉碎预处理后得香蕉皮粉,将香蕉皮粉与水以1:40的质量比混合,得粉浆;1) Take fresh and healthy banana peels, wash, dry, and pulverize them to obtain banana peel powder, mix the banana peel powder with water at a mass ratio of 1:40, and obtain a slurry;
2)将粉浆与香蕉皮粉原料质量1.5%质量的维生素C混合,煮沸灭酶2.5min;加入1mol/L NaOH溶液调节pH至7,然后每kg香蕉皮粉原料加入8g淀粉酶于80℃酶解75min;再次加入1mol/L NaOH溶液调节pH至7,然后每kg香蕉皮粉原料加入胰蛋白酶6g于55℃酶解115min,得酶解液;2) Mix the powder slurry with 1.5% vitamin C of banana peel powder raw material, boil to inactivate the enzyme for 2.5 minutes; add 1mol/L NaOH solution to adjust the pH to 7, then add 8g of amylase per kg of banana peel powder raw material at 80°C Enzyme hydrolyze for 75 minutes; add 1mol/L NaOH solution again to adjust the pH to 7, then add 6g of trypsin per kg of banana peel powder raw material and enzymolyze at 55°C for 115min to obtain an enzymolyzed solution;
3)将酶解液离心分离后取上清液,将上清液置于旋转蒸发仪中浓缩至原体积的1/4,旋转蒸发参数为:温度55℃,转速50rmp;然后将浓缩液与4.5倍的无水乙醇混合醇析3h,得醇析物;3) After centrifuging the enzymatic solution, take the supernatant, put the supernatant in a rotary evaporator and concentrate it to 1/4 of the original volume, the parameters of the rotary evaporation are: temperature 55°C, speed 50rmp; 4.5 times of absolute ethanol was mixed with ethanol for 3 hours to obtain alcohol analyte;
4)将醇析物过滤得滤饼,将滤饼干燥后,超微粉碎,超微粉碎,然后过150目筛,即得香蕉皮膳食纤维。4) Filter the alcohol analyte to obtain a filter cake, dry the filter cake, ultrafinely pulverize, and then pass through a 150-mesh sieve to obtain the banana peel dietary fiber.
本实施例中的低糖酸奶由以下重量百分含量的原料制成:复原乳75%,香蕉皮膳食纤维3%,糖液17%,5%嗜热链球菌与保加利亚乳杆菌混合菌种;所述糖液由质量分数为55%木糖醇、15%低聚木糖与30%水组成。The low-sugar yogurt in this example is made from the following raw materials in percentage by weight: reconstituted milk 75%, banana peel dietary fiber 3%, sugar liquid 17%, 5% mixed strains of Streptococcus thermophilus and Lactobacillus bulgaricus; The sugar solution is composed of 55% xylitol, 15% xylooligosaccharide and 30% water in mass fraction.
所述嗜热链球菌与保加利亚乳杆菌的质量比为1:1。所述混合菌种中活菌数大于等于100万cfu/g。The mass ratio of Streptococcus thermophilus to Lactobacillus bulgaricus is 1:1. The number of viable bacteria in the mixed strain is greater than or equal to 1 million cfu/g.
所述复原乳由以下重量百分含量的组分组成:20%的全脂乳粉,8%的脱脂乳粉,72%的水。取配方量的全脂奶粉、脱脂奶粉加入配方量的水搅拌使其均匀溶解,即得复原乳。The reconstituted milk is composed of the following components in percentage by weight: 20% whole milk powder, 8% skimmed milk powder, and 72% water. Take the formula amount of whole milk powder and skim milk powder, add the formula amount of water and stir to dissolve evenly, and then obtain the reconstituted milk.
上述的低糖酸奶的制备方法,包括如下步骤:取配方量的木糖醇、低聚木糖与水于50℃混合,得糖液;取配方量的复原乳、香蕉皮膳食纤维、所述糖液混合,于70℃、15MPa下均质处理,得混合液;将混合液于85℃灭菌20min;然后冷却至45℃,接入菌种于45℃下发酵3h;冷却至室温,然后于4℃放置12h,即得。The preparation method of the above-mentioned low-sugar yoghurt comprises the following steps: taking xylitol and xylooligosaccharide of formula quantity and mixing with water at 50°C to obtain sugar liquid; taking formula quantity of reconstituted milk, banana peel dietary fiber, the sugar mixed solution, and homogeneously treated at 70°C and 15MPa to obtain a mixed solution; sterilized the mixed solution at 85°C for 20 minutes; then cooled to 45°C, added strains and fermented at 45°C for 3 hours; cooled to room temperature, and then Place it at 4°C for 12h, and it is ready.
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