CN104152521B - Peony pollen polypeptide and preparation method and application - Google Patents
Peony pollen polypeptide and preparation method and application Download PDFInfo
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- CN104152521B CN104152521B CN201410399776.5A CN201410399776A CN104152521B CN 104152521 B CN104152521 B CN 104152521B CN 201410399776 A CN201410399776 A CN 201410399776A CN 104152521 B CN104152521 B CN 104152521B
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Abstract
The method of peony pollen polypeptide is prepared as raw material using peony pollen and the application of peony pollen polypeptide and the polypeptide that this method is prepared the present invention relates to a kind of.Peony pollen polypeptide of the invention is peony pollen albumen or peony pollen polypeptide.Using dry peony pollen as raw material, is extracted by pollen broken wall, crude protein, vacuum drying, obtain peony pollen albumen；Peony pollen albumen is passed through into proteolysis, debitterizing and decoloring, centrifugation or filtering, obtains peony pollen polypeptide.The product can be the forms such as pulvis, particle, tablet, capsule, oral solution.Tree peony polypeptide have reduce cholesterol, blood pressure lowering and promote fat metabolism, it is antifatigue, enhance human immunity, adjust human physiological functions and other effects advantage, in food industry, health care and medicine and other fields with wide development and application prospect.
Description
Technical field
The invention belongs to peony pollen product technique fields, and in particular to a kind of peony pollen polypeptide, preparation method
And its application.
Background technique
Tree peony (Paeonia suffruticosa Andr.) belongs to Ranunculaceae Paeonia, and machaka has very high sight
Reward value and pharmaceutical value.For a long time, the development and utilization of tree peony deep processed product but seem serious lag that production added value is very
It is low, do not form industrial chain.Peony pollen is not only full of nutrition but also contains various bioactivators.Amino acid content exists
25.0% or more, rich in there is 17 kinds of amino acid, vitamin B, Vitamin C content are abundant, also containing tree peony polysaccharide, tree peony flavones etc.
Bioactive substance.Protein content 39.3% is known as protein compression by academia much higher than the protein content of general pollen
The hat of contracting body, native protein.
Peony pollen polypeptide refers to the protein in peony pollen through protease hydrolytic, the small molecule for separating, being refining to obtain
Oligopeptide mixture.Natural pollen has tough and tensile cell wall, and the sporopollenin in structure has acidproof, alkaline-resisting, heatproof, pressure resistance
And the physicochemical property highly stable to gastric acid and other digestive system enzymes, human body is hindered to the absorption benefit of pollen protein
With.Broken wall is carried out to pollen, the absorption of protein can be improved.Therefore, in order to make full use of peony pollen resource, using suitable
Production technology these vegetable proteins are utilized, be converted into polypeptide and isolated and purified, albumen or health-care polypeptide product is made, it is right
The added value and the market competitiveness for improving tree peony industry are of great significance.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of preparation method of peony pollen polypeptide, party's legal systems
Standby peony pollen protein polypeptide preparation and related application.
The present invention provides a kind of peony pollen polypeptide, is prepared as a raw material with dry peony pollen.
Further, the peony pollen derives from phoenix pellet tree peony or Paeonia papaveracea.
Further, the peony pollen polypeptide is peony pollen albumen or peony pollen polypeptide.
The present invention also provides a kind of preparation methods of peony pollen polypeptide, which is characterized in that the peony pollen egg
White polypeptide is prepared as a raw material with dry peony pollen.Further, the peony pollen derives from phoenix pellet tree peony or purple
Spot tree peony.
Further, the peony pollen polypeptide is using dry peony pollen as raw material, by pollen broken wall, crude protein
It extracts, vacuum drying, obtains peony pollen albumen.Or further, peony pollen albumen is de- by proteolysis, de- hardship
Color, centrifugation or filtering obtain peony pollen polypeptide.
Further, the method further includes the steps that ultrafiltration membrane refines after centrifugation or filtering.
Further, the method further includes the steps that dry, sterilizing after refining.
Further, the pollen broken wall is ultramicronising broken wall or whirlwind comminuting method broken wall.
Preferably, ultramicronising broken wall is peony pollen planetary ball mill ultramicronising broken wall, revolving speed 400-600r/
Min, grinding time 0.5-1.5h, ratio of grinding media to material 6.8-7.Whirlwind comminuting method broken wall is using whirlwind comminuting method, by tree peony
Pollen is put into cyclone type high speed sample pulverizer and crushes 1-5min.
Further, the crude protein is extracted as aqueous soluble protein extraction, salting-in-protein extracts, alcohol soluble protein extracts or alkali
Molten protein extraction.
Preferably, aqueous soluble protein extracts, and is the pollen after broken wall, and distilled water, 4-60 is added by material-water ratio 1:10-20
DEG C stirring extract 1-8h, and through 4000-8000r/min be centrifuged 10-20min after collect supernatant, residue is spare.In supernatant
In a certain amount of solid ammonium sulfate is added step by step, so that ammonium sulfate saturation degree is successively reached 40%, 60%, 80%, be sufficiently stirred every time
After 1h, 4000-8000r/min are centrifuged 10-20min, precipitatings at different levels are collected, are dissolved with the phosphate buffer of pH5.0-7.0, so
Ultrafiltration is carried out with the ultrafiltration membrane that trapped molecular weight is 100KDa afterwards, obtains water-soluble crude protein solution.
Preferably, further comprise that salting-in-protein extracts, be by the residue after above-mentioned extraction with aqueous solution, by solid-liquid ratio
0.15-0.50mol/L sodium chloride solution is added in 1:10-20, and 1-8h is extracted in 4-60 DEG C of stirring, and is centrifuged through 4000-8000r/min
Supernatant is collected after 10-20min, residue is spare.A certain amount of solid ammonium sulfate is added in supernatant, makes ammonium sulfate saturation degree
Reach 40%, 1h is sufficiently stirred, after 4000-8000r/min is centrifuged 10-20min, precipitating is collected, with the chlorine of above-mentioned comparable sodium
Change sodium solution dissolution, then carries out ultrafiltration with the ultrafiltration membrane that trapped molecular weight is 100KDa, obtain the molten crude protein solution of salt.
Preferably, further comprise that alcohol soluble protein extracts, be the residue after extracting above-mentioned sodium chloride solution, by material
75% ethanol solution is added in liquor ratio 1:10-20, and 1-8h is extracted in 4-60 DEG C of stirring, and is centrifuged 10-20min through 4000-8000r/min
After collect supernatant, residue is spare.Distilled water is added by material-water ratio 1:20-30 in supernatant, 1h, 4000- is sufficiently stirred
After 8000r/min is centrifuged 10-20min, precipitating is collected, is dissolved with 75% ethyl alcohol, with after distilled water dialysis for 24 hours at 4 DEG C, obtains alcohol
Molten crude protein precipitating.
Preferably, further comprise that alkali-soluble protein extracts, be the residue after extracting above-mentioned ethanol solution, by feed liquid
Be added 0.10-0.75mol/L NaOH solution than 1:10-20,4-60 DEG C of stirring extraction 1-8h, and through 4000-8000r/min from
Supernatant is collected after heart 10-20min.A certain amount of solid ammonium sulfate is added in supernatant, ammonium sulfate saturation degree is made to reach 40%,
1h is sufficiently stirred, after 4000-8000r/min is centrifuged 10-20min, collects precipitating, is dissolved with the NaOH of above-mentioned comparable sodium, so
Ultrafiltration is carried out with the ultrafiltration membrane that trapped molecular weight is 100KDa afterwards, obtains alkali soluble crude protein solution.
Further, the vacuum freeze drying is above-mentioned water-soluble crude protein solution, the molten crude protein solution of salt, alcohol
Molten crude protein precipitating, alkali soluble crude protein solution, vacuum freeze drying obtains peony at a temperature of -55 DEG C~-40 DEG C respectively
Powder aqueous soluble protein, salting-in-protein, alcohol soluble protein, alkali-soluble protein.
The present invention also provides a kind of peony pollen albumen, are peony pollen aqueous soluble protein, peony pollen salting-in-protein, tree peony
One of pollen alcohol soluble protein, peony pollen alkali-soluble protein are several.
Further, the proteolysis is for above-mentioned peony pollen albumen to be added by material-water ratio 1:10-20 and distill
Water adds the protease of peony pollen albumen butt 3-10%, and 35-55 DEG C of heat preservation digests 2-12h, then is warming up to 80-95 DEG C of height
Warm enzyme deactivation 8-15min.
Preferably, the protease is selected from papain, flavor protease, alkali protease, neutral proteinase etc.
One of or it is several.
It is furthermore preferred that the protease is the combination of alkali protease or flavor protease and neutral proteinase, or
The combination of person's alkali protease and neutral proteinase or the combination of alkali protease and papain.
Further, the debitterizing and decoloring is to add stir process 30- at 40-65 DEG C of 0.1-0.5% active carbon
40min。
Further, the centrifugation or filtering are 4000-10000r/min centrifugation 20-25min or plate-frame filtering.
Further, the described ultrafiltration refining, be the peony pollen polypeptide ultrafiltration membrane that will be centrifuged or be obtained by filtration by
Molecular weight carries out classification separation, obtains different molecular weight peony pollen polypeptide solution；Molecular cut off be 10Kda hereinafter, it is preferred that
5Kda hereinafter, more preferably 3Kda hereinafter, most preferably 1Kda or less.
Further, it is spray drying or freeze-drying that the peony pollen polypeptide solution is dry.
Preferably, spray drying is peony pollen polypeptide solution, the 70-130 DEG C of spray under 0.08-0.2 atmospheric pressure
Mist is dry, obtains peony pollen polypeptide.Freeze-drying is at a temperature of -55 DEG C~-40 DEG C, using vacuum freeze drying, obtains
Peony pollen polypeptide.
The present invention also provides a kind of peony pollen polypeptides, are prepared by any of the above-described method.
The present invention also provides a kind of peony pollen protein polypeptide preparations, which is characterized in that direct by above-mentioned polypeptide
It is prepared, or, which is characterized in that the polypeptide is powder or is further processed into particle, tablet, capsule, takes orally
The forms such as liquid.
The present invention also provides the application of a kind of above-mentioned peony pollen polypeptide or preparation, the application includes as battalion
Support food, health food, arsenic or protein beverage.
Preferably, the nutraceutical is the nutraceutical provided for patient or baby；The health food be old
The health food that year people or sub-health population provide, the polypeptide beverage refer to the independent Instant Drinks of peony pollen polypeptide
Or companion's Instant Drinks as existing beverage.
The peony pollen polypeptide that the method for the present invention is prepared have good nutritive peculiarity, absorption easy to digest, especially
It is certain low molecular peptides, can not only provide human body energy rapidly, and have unique processing performance, can be used as nutrition
Food, arsenic and protein beverage.Meanwhile peony pollen polypeptide has reduction cholesterol, blood pressure lowering and promotes rouge
Fat metabolism, it is antifatigue, enhance human immunity, adjust human physiological functions and other effects advantage, food industry, health food and
Medicine and other fields have wide development and application prospect.
Specific embodiment
The present invention is further illustrated below with reference to embodiment, it should be understood that these examples cannot function as limitation of the invention,
Without departing from the spirit and substance of the case in the present invention, made modifications or substitutions all belong to the scope of the present invention.If not especially
It indicates, the means in following embodiments are conventional means known in the art.
1 peony pollen broken wall of embodiment
With planetary ball mill to pollen ultramicronising broken wall, revolving speed 600r/min, grinding time 0.5h, ratio of grinding media to material 7.Through
Measurement, the rate of breaking pollen wall of the present embodiment are 100%.
2 peony pollen broken wall of embodiment
With planetary ball mill to pollen ultramicronising broken wall, revolving speed 400r/min, grinding time 1.5h, ratio of grinding media to material 6.8.
After measured, the rate of breaking pollen wall of the present embodiment is 96%.
3 peony pollen broken wall of embodiment
Pollen is put into crushing 5min broken wall in cyclone type high speed sample pulverizer.After measured, the pollen of the present embodiment is broken
Wall rate is 100%.
4 peony pollen broken wall of embodiment
Pollen is put into crushing 1min broken wall in cyclone type high speed sample pulverizer.After measured, the pollen of the present embodiment is broken
Wall rate is 94%.
The preparation of 5 peony pollen aqueous soluble protein of embodiment
Distilled water is added by material-water ratio 1:10 in the step 1 peony pollen that embodiment 1-4 is any, and 4 DEG C of stirrings are extracted
8h, and supernatant is collected after 4000r/min is centrifuged 20min, residue is spare.A certain amount of solid is added step by step in supernatant
Ammonium sulfate makes ammonium sulfate saturation degree successively reach 40%, 60%, 80%, and 1h is sufficiently stirred every time, and 8000r/min is centrifuged 10min
Afterwards, precipitatings at different levels are collected, are dissolved with the phosphate buffer of pH7.0, the ultrafiltration membrane for being then 100KDa with trapped molecular weight
Ultrafiltration is carried out, water-soluble crude protein solution is obtained.
Step 2 is at a temperature of -55 DEG C, vacuum freeze drying, obtains peony pollen aqueous soluble protein.
The preparation of 6 peony pollen aqueous soluble protein of embodiment
Distilled water is added by material-water ratio 1:20 in the step 1 peony pollen that embodiment 1-4 is any, and 60 DEG C of stirrings are extracted
1h, and supernatant is collected after 8000r/min is centrifuged 10min, residue is spare.A certain amount of solid is added step by step in supernatant
Ammonium sulfate makes ammonium sulfate saturation degree successively reach 40%, 60%, 80%, and 1h is sufficiently stirred every time, and 4000r/min is centrifuged 20min
Afterwards, precipitatings at different levels are collected, are dissolved with the phosphate buffer of pH5.0, the ultrafiltration membrane for being then 100KDa with trapped molecular weight
Ultrafiltration is carried out, water-soluble crude protein solution is obtained.
Step 2 is at a temperature of -40 DEG C, vacuum freeze drying, obtains peony pollen aqueous soluble protein.
The preparation of 7 peony pollen salting-in-protein of embodiment
0.15mol/ is added by the residue after embodiment 5-6 any extraction with aqueous solution, by solid-liquid ratio 1:10 in step 1
L sodium chloride solution, 8h is extracted in 4 DEG C of stirrings, and collects supernatant after 4000r/min is centrifuged 20min, and residue is spare.Upper
A certain amount of solid ammonium sulfate is added in clear liquid, ammonium sulfate saturation degree is made to reach 40%, 1h, 8000r/min centrifugation is sufficiently stirred
After 10min, precipitating is collected, is dissolved with 0.15mol/L sodium chloride solution, the ultrafiltration membrane for being then 100KDa with trapped molecular weight
Ultrafiltration is carried out, the molten crude protein solution of salt is obtained.
Step 2 is at a temperature of -40 DEG C, vacuum freeze drying, obtains peony pollen salting-in-protein.
The preparation of 8 peony pollen salting-in-protein of embodiment
0.50mol/ is added by the residue after embodiment 5-6 any extraction with aqueous solution, by solid-liquid ratio 1:20 in step 1
L sodium chloride solution, 1h is extracted in 60 DEG C of stirrings, and collects supernatant after 8000r/min is centrifuged 10min, and residue is spare.Upper
A certain amount of solid ammonium sulfate is added in clear liquid, ammonium sulfate saturation degree is made to reach 40%, 1h, 4000r/min centrifugation is sufficiently stirred
After 20min, precipitating is collected, is dissolved with 0.50mol/L sodium chloride solution, the ultrafiltration membrane for being then 100KDa with trapped molecular weight
Ultrafiltration is carried out, the molten crude protein solution of salt is obtained.
Step 2 is at a temperature of -55 DEG C, vacuum freeze drying, obtains peony pollen salting-in-protein.
The preparation of 9 peony pollen alcohol soluble protein of embodiment
Residue after embodiment 7-8 any sodium chloride solution extraction is added 75% by solid-liquid ratio 1:10 by step 1
Ethanol solution, 8h is extracted in 4 DEG C of stirrings, and collects supernatant after 4000r/min is centrifuged 20min, and residue is spare.In supernatant
In by material-water ratio 1:30 distilled water is added, be sufficiently stirred 1h, after 8000r/min is centrifuged 10min, precipitating collected, with 75% ethyl alcohol
It dissolves, with after distilled water dialysis for 24 hours at 4 DEG C, obtains the molten crude protein of alcohol and precipitate.
Step 2 is at a temperature of -55 DEG C, vacuum freeze drying, obtains peony pollen alcohol soluble protein.
The preparation of 10 peony pollen alcohol soluble protein of embodiment
Residue after embodiment 7-8 any sodium chloride solution extraction is added 75% by solid-liquid ratio 1:20 by step 1
Ethanol solution, 1h is extracted in 60 DEG C of stirrings, and collects supernatant after 8000r/min is centrifuged 10min, and residue is spare.In supernatant
Distilled water is added by material-water ratio 1:20 in liquid, 1h is sufficiently stirred, after 4000r/min is centrifuged 20min, precipitating is collected, with 75% second
Alcohol dissolves, and with after distilled water dialysis for 24 hours at 4 DEG C, obtains the molten crude protein of alcohol and precipitates.
Step 2 is at a temperature of -40 DEG C, vacuum freeze drying, obtains peony pollen alcohol soluble protein.
The preparation of 11 peony pollen alkali-soluble protein of embodiment
Residue after embodiment 9-10 any ethanol solution extraction is added step 1 by solid-liquid ratio 1:10
0.10mol/L NaOH solution, 8h is extracted in 4 DEG C of stirrings, and collects supernatant after 4000r/min is centrifuged 20min.In supernatant
It is middle that a certain amount of solid ammonium sulfate is added, so that ammonium sulfate saturation degree is reached 40%, 1h is sufficiently stirred, 8000r/min is centrifuged 10min
Afterwards, precipitating is collected, is dissolved with 0.10mol/L NaOH, then carries out ultrafiltration with the ultrafiltration membrane that trapped molecular weight is 100KDa,
Obtain alkali soluble crude protein solution.
Step 2 is at a temperature of -40 DEG C, vacuum freeze drying, obtains peony pollen alkali-soluble protein.
The preparation of 12 peony pollen alkali-soluble protein of embodiment
Residue after embodiment 9-10 any ethanol solution extraction is added step 1 by solid-liquid ratio 1:20
0.75mol/L NaOH solution, 1h is extracted in 60 DEG C of stirrings, and collects supernatant after 8000r/min is centrifuged 10min.In supernatant
It is middle that a certain amount of solid ammonium sulfate is added, so that ammonium sulfate saturation degree is reached 40%, 1h is sufficiently stirred, 4000r/min is centrifuged 20min
Afterwards, precipitating is collected, is dissolved with 0.75mol/L NaOH, then carries out ultrafiltration with the ultrafiltration membrane that trapped molecular weight is 100KDa,
Obtain alkali soluble crude protein solution.
Step 2 is at a temperature of -55 DEG C, vacuum freeze drying, obtains peony pollen alkali-soluble protein.
The preparation of 13 peony pollen albumen of embodiment
By embodiment 5-6 any peony pollen aqueous soluble protein, embodiment 7-8 any peony pollen salting-in-protein, reality
Any peony pollen alkali-soluble protein of any peony pollen alcohol soluble protein of a 9-10, embodiment 11-12 is applied, chooses 2 kinds or 2 kinds
The above mixing, is made peony pollen albumen.
The preparation of 14 peony pollen polypeptide of embodiment
Step 1 digests the peony pollen albumen that embodiment 5-13 is any, and distilled water is added by material-water ratio 1:10, adds
Add the alkali protease of peony pollen albumen butt 3%, adjusting pH is that 10.0,50 DEG C of heat preservations digest 2h.
After step 2 enzyme deactivation waits digesting, degradation product is heated rapidly to 80 DEG C, enzyme deactivation 15min is carried out to enzymolysis liquid.
0.5% active carbon stir process 30min is added when enzymolysis liquid is cooled to 40 DEG C in step 3 debitterizing and decoloring.
The separation of step 4 filtrate removes the solid impurity in enzymolysis liquid by flame filter press, to obtain peony pollen
Polypeptide coarse filtration liquid.
Peony pollen polypeptide coarse filtration liquid is carried out classification separation by molecular weight with ultrafiltration membrane by step 5 refining, retains molecule
Amount is 10KDa.
Step 6 is dry under 0.15 atmospheric pressure, 90 DEG C of spray drying.
Step 7 140 DEG C of high-temperature short-time sterilization 8s of sterilizing, obtain peony pollen polypeptide.
With peony pollen aqueous soluble protein (embodiment 5), peony pollen salting-in-protein (embodiment 7), tree peony in embodiment 13
The peony pollen albumen that 4 kinds of pollen alcohol soluble protein (embodiment 9), peony pollen alkali-soluble protein (embodiment 11) albumen are mixed to prepare
For, after measured, the degree of hydrolysis of peony pollen albumen is 42.3%, and obtained polypeptide product is without bitter taste.
Said products are directly as nutraceutical, health food, arsenic or protein beverage.
The preparation of 15 peony pollen polypeptide of embodiment
Step 1 digests the peony pollen albumen that embodiment 5-13 is any, and distilled water is added by material-water ratio 1:15, adds
Add the papain of peony pollen albumen butt 8%, adjusting pH is that 6.5,45 DEG C of heat preservations digest 3h.
After step 2 enzyme deactivation waits digesting, degradation product is heated rapidly to 95 DEG C, enzyme deactivation 8min is carried out to enzymolysis liquid.
0.2% active carbon stir process 40min is added when enzymolysis liquid is cooled to 50 DEG C in step 3 debitterizing and decoloring.
Step 4 filtrate separates 4000r/min and is centrifuged 25min, supernatant is taken, to obtain peony pollen polypeptide coarse filtration
Liquid.
Peony pollen polypeptide coarse filtration liquid is carried out classification separation by molecular weight with ultrafiltration membrane by step 5 refining, retains molecule
Amount is 5KDa.
Step 6 is dry under 0.2 atmospheric pressure, 80 DEG C of spray drying.
Step 7 140 DEG C of high-temperature short-time sterilization 8s of sterilizing, obtain peony pollen polypeptide.
With peony pollen aqueous soluble protein (embodiment 5), peony pollen salting-in-protein (embodiment 7), tree peony in embodiment 13
The peony pollen albumen that 4 kinds of pollen alcohol soluble protein (embodiment 9), peony pollen alkali-soluble protein (embodiment 11) albumen are mixed to prepare
For, after measured, the degree of hydrolysis of peony pollen albumen is 28.1%, and obtained polypeptide product is without bitter taste.
Said products are directly as nutraceutical, health food, arsenic or protein beverage.
The preparation of 16 peony pollen polypeptide of embodiment
Step 1 digests the peony pollen albumen that embodiment 5-13 is any, and distilled water is added by material-water ratio 1:20, adds
Add the neutral proteinase of peony pollen albumen butt 10%, adjusting pH is that 7,40 DEG C of heat preservations digest 3h.
After step 2 enzyme deactivation waits digesting, degradation product is heated rapidly to 80 DEG C, enzyme deactivation 15min is carried out to enzymolysis liquid.
0.1% active carbon stir process 30min is added when enzymolysis liquid is cooled to 65 DEG C in step 3 debitterizing and decoloring.
Step 4 filtrate separates 10000r/min and is centrifuged 20min, supernatant is taken, to obtain peony pollen polypeptide coarse filtration
Liquid.
Peony pollen polypeptide coarse filtration liquid is carried out classification separation by molecular weight with ultrafiltration membrane by step 5 refining, retains molecule
Amount is 3KDa.
Step 6 is dry at a temperature of -55 DEG C, vacuum freeze drying.
Step 7 140 DEG C of high-temperature short-time sterilization 8s of sterilizing, obtain peony pollen polypeptide.
With peony pollen aqueous soluble protein (embodiment 5), peony pollen salting-in-protein (embodiment 7), tree peony in embodiment 13
The peony pollen albumen that 4 kinds of pollen alcohol soluble protein (embodiment 9), peony pollen alkali-soluble protein (embodiment 11) albumen are mixed to prepare
For, after measured, the degree of hydrolysis of peony pollen albumen is 30.4%, and obtained polypeptide product is without bitter taste.
Said products are directly as nutraceutical, health food, arsenic or protein beverage.
The preparation of 17 peony pollen polypeptide of embodiment
Step 1 digests the peony pollen albumen that embodiment 5-13 is any, and distilled water is added by material-water ratio 1:20, adds
Add the flavor protease of peony pollen albumen butt 8%, adjusting pH is that 6,50 DEG C of heat preservations digest 12h.
After step 2 enzyme deactivation waits digesting, degradation product is heated rapidly to 95 DEG C, enzyme deactivation 8min is carried out to enzymolysis liquid.
0.5% active carbon stir process 30min is added when enzymolysis liquid is cooled to 40 DEG C in step 3 debitterizing and decoloring.
The separation of step 4 filtrate removes the solid impurity in enzymolysis liquid by flame filter press, to obtain peony pollen
Polypeptide coarse filtration liquid.
Peony pollen polypeptide coarse filtration liquid is carried out classification separation by molecular weight with ultrafiltration membrane by step 5 refining, retains molecule
Amount is 5KDa.
Step 6 is dry at a temperature of -40 DEG C, vacuum freeze drying.
Step 7 140 DEG C of high-temperature short-time sterilization 8s of sterilizing, obtain peony pollen polypeptide.
With peony pollen aqueous soluble protein (embodiment 5), peony pollen salting-in-protein (embodiment 7), tree peony in embodiment 13
The peony pollen albumen that 4 kinds of pollen alcohol soluble protein (embodiment 9), peony pollen alkali-soluble protein (embodiment 11) albumen are mixed to prepare
For, after measured, the degree of hydrolysis of peony pollen protein is 33.3%, and obtained polypeptide product is without bitter taste.
Said products are directly as nutraceutical, health food, arsenic or protein beverage.
The preparation of 18 peony pollen polypeptide of embodiment
Step 1 digests the peony pollen albumen that embodiment 5-13 is any, and distilled water is added by material-water ratio 1:10, adds
Add the mixing protease of peony pollen albumen butt 5%, the mixing protease is alkali protease: neutral proteinase mass ratio
For 1:9, adjusting pH is that 9,45 DEG C of heat preservations digest 2h.
After step 2 enzyme deactivation waits digesting, degradation product is heated rapidly to 80 DEG C, enzyme deactivation 15min is carried out to enzymolysis liquid.
0.2% active carbon stir process 40min is added when enzymolysis liquid is cooled to 50 DEG C in step 3 debitterizing and decoloring.
Step 4 filtrate separates 4000r/min and is centrifuged 25min, supernatant is taken, to obtain peony pollen polypeptide coarse filtration
Liquid.
Peony pollen polypeptide coarse filtration liquid is carried out classification separation by molecular weight with ultrafiltration membrane by step 5 refining, retains molecule
Amount is 10KDa.
Step 6 is dry under 0.15 atmospheric pressure, 90 DEG C of spray drying.
Step 7 140 DEG C of high-temperature short-time sterilization 8s of sterilizing, obtain peony pollen polypeptide.
With peony pollen aqueous soluble protein (embodiment 5), peony pollen salting-in-protein (embodiment 7), tree peony in embodiment 13
The peony pollen albumen that 4 kinds of pollen alcohol soluble protein (embodiment 9), peony pollen alkali-soluble protein (embodiment 11) albumen are mixed to prepare
For, after measured, the degree of hydrolysis of peony pollen protein is 45.7%, and obtained polypeptide product is without bitter taste.
Said products are directly as nutraceutical, health food, arsenic or protein beverage.
The preparation of 19 peony pollen polypeptide of embodiment
Step 1 digests the peony pollen albumen that embodiment 5-13 is any, and distilled water is added by material-water ratio 1:15, adds
Add the mixing protease of peony pollen albumen butt 8%, the mixing protease is alkali protease: papain mass ratio
For 1:1, adjusting pH is that 9,45 DEG C of heat preservations digest 2h.
After step 2 enzyme deactivation waits digesting, degradation product is heated rapidly to 95 DEG C, enzyme deactivation 8min is carried out to enzymolysis liquid.
0.1% active carbon stir process 30min is added when enzymolysis liquid is cooled to 65 DEG C in step 3 debitterizing and decoloring.
Step 4 filtrate separates 10000r/min and is centrifuged 20min, supernatant is taken, to obtain peony pollen polypeptide coarse filtration
Liquid.
Peony pollen polypeptide coarse filtration liquid is carried out classification separation by molecular weight with ultrafiltration membrane by step 5 refining, retains molecule
Amount is 3KDa.
Step 6 is dry at a temperature of -40 DEG C, vacuum freeze drying.
Step 7 140 DEG C of high-temperature short-time sterilization 8s of sterilizing, obtain peony pollen polypeptide.
With peony pollen aqueous soluble protein (embodiment 5), peony pollen salting-in-protein (embodiment 7), tree peony in embodiment 13
The peony pollen albumen that 4 kinds of pollen alcohol soluble protein (embodiment 9), peony pollen alkali-soluble protein (embodiment 11) albumen are mixed to prepare
For, after measured, the degree of hydrolysis of peony pollen protein is 38.1%, and obtained polypeptide product is without bitter taste.
Said products are directly as nutraceutical, health food, arsenic or protein beverage.
The preparation of 20 peony pollen polypeptide of embodiment
Step 1 digests the peony pollen albumen that embodiment 5-13 is any, and distilled water is added by material-water ratio 1:20, adds
Add the mixing protease of peony pollen albumen butt 10%, the mixing protease is flavor protease: neutral proteinase quality
Than being that 7,40 DEG C of heat preservations digest 12h for 2:1, tune pH.
After step 2 enzyme deactivation waits digesting, degradation product is heated rapidly to 80 DEG C, enzyme deactivation 15min is carried out to enzymolysis liquid.
0.5% active carbon stir process 30min is added when enzymolysis liquid is cooled to 40 DEG C in step 3 debitterizing and decoloring.
The separation of step 4 filtrate removes the solid impurity in enzymolysis liquid by flame filter press, to obtain peony pollen
Polypeptide coarse filtration liquid.
Peony pollen polypeptide coarse filtration liquid is carried out classification separation by molecular weight with ultrafiltration membrane by step 5 refining, retains molecule
Amount is 10KDa.
Step 6 is dry at a temperature of -55 DEG C, vacuum freeze drying.
Step 7 140 DEG C of high-temperature short-time sterilization 8s of sterilizing, obtain peony pollen polypeptide.
With peony pollen aqueous soluble protein (embodiment 5), peony pollen salting-in-protein (embodiment 7), tree peony in embodiment 13
The peony pollen albumen that 4 kinds of pollen alcohol soluble protein (embodiment 9), peony pollen alkali-soluble protein (embodiment 11) albumen are mixed to prepare
For, after measured, the degree of hydrolysis of peony pollen protein is 40%, and obtained polypeptide product is without bitter taste.
Said products are directly as nutraceutical, health food, arsenic or protein beverage.
The preparation of 21 tree peony polypeptide particles agent of embodiment
Each component is weighed according to following weight percents: embodiment 5-20 any tree peony polypeptide powder 35.0%,
Sucrose 45.0%, converted starch 16.0%, citric acid 2.0%, malic acid 2.0%.
First above-mentioned each material is crushed or is sieved；Material after successively weighing crushing or sieving by formula ratio；It will claim
Measured material puts into mixing machine, is uniformly mixed；Suitable alcohols are added, are pelletized by granulator；The particle made is used
Drying equipment is dried, and then carries out whole grain by pelletizing machine；It finally dispenses, obtains peony pollen egg of the present invention
White polypeptide particles agent.
The preparation of 22 tree peony polypeptide tablet of embodiment
Each component is weighed according to following weight percents: embodiment 5-20 any tree peony polypeptide powder 35.0%, sugarcane
Sugared 28.5%, starch 10.0%, coated vitamin C 1.5%, microcrystalline cellulose 15.0%, dextrin 8.0%, film coating agent
1.0%, magnesium stearate 0.6%, silica 0.4%.
First above-mentioned each material is crushed or is sieved；Material after successively weighing crushing or sieving by formula ratio；It will claim
Measured material puts into mixing machine, is uniformly mixed；Suitable alcohols are added, are pelletized by granulator；The particle made is used
Drying equipment is dried, and then carries out whole grain by pelletizing machine；Magnesium stearate, silica progress total mix is added, by total mix
Material after uniformly carries out tabletting by tablet press machine, and plain piece is temporary, spare；Film coating agent is added in suitable quantity of water, stirring is equal
It is even, it is configured to the coating solution that concentration is 5.0-15.0%, processing is coated to the plain piece pressed by seed-coating machine；Finally divide
Dress, obtains the tablet of peony pollen polypeptide of the present invention.
The preparation of 23 tree peony polypeptide capsule of embodiment
Each component is weighed according to following weight percents: embodiment 5-20 any tree peony polypeptide powder 54.0%,
Coated vitamin C 9.0%, microcrystalline cellulose 36.0%, magnesium stearate 0.6%, silica 0.4%.
First above-mentioned each material is crushed, is sieved；The material after crushing, sieving is successively weighed by formula ratio；It will weigh
Good material puts into mixing machine, is uniformly mixed；Then capsule is filled by capsule filler；It finally dispenses, obtains
Peony pollen polypeptide capsule of the present invention.
The preparation of 24 tree peony polypeptide oral liquor of embodiment
Each component is weighed according to following weight percents: embodiment 5-20 any tree peony polypeptide powder 50.0%, bee
Honey 10.0%, vitamin C sodium salt 5%, malic acid 2.0%, citric acid 1.6%, carragheen 1.0%, stevioside 0.2%, peace
Match honey 0.2%, 30% is pure to use water.
Above-mentioned each material is weighed by formula ratio, is put into water, is sufficiently dissolved；Then acquired solution oral solution is transferred to fill
In installation, inject in container；Sterilizing, obtains peony pollen polypeptide oral solution of the present invention.
One, in order to compare the variation of Major Nutrient substance before and after peony pollen broken wall, our broken walls to broken pollen and not
Pollen crude protein, amino acid, total starches, general flavone equal size are evaluated.
Table 1: tree peony broken wall and not broken pollen comparision contents
| Sample | Sporoderm-broken rate % | Crude protein g/100g | Amino acid g/100g | Total starches g/100g | General flavone g/100g |
| Embodiment 1 | 100 | 39.8 | 31.3 | 2.92 | 2.73 |
| Embodiment 2 | 96 | 37.5 | 30.9 | 2.65 | 2.54 |
| Embodiment 3 | 100 | 39.1 | 32.8 | 2.85 | 2.68 |
| Embodiment 4 | 94 | 35.9 | 30.4 | 2.51 | 2.42 |
| Common peony pollen (control) | 0 | 32.3 | 25.1 | 1.94 | 2.02 |
Index explanation:
1, the calculating of rate of breaking pollen wall
Configuration suspension: the control pollen of natural drying and each about 10mg of pollen of broken wall treatment are accurately weighed, is set respectively
In the mortar of dried and clean, it is added 1ml chloraldurate test solution (taking chloraldurate 50g, add water 15ml and glycerol 10ml miscible),
It is lightly ground to being uniformly dispersed, immediately load.Film-making: selecting a capillary to cross at suitable position, as scale mark,
Suspension load is quantitatively drawn with this root capillary tube, is covered with 18 × 18mm coverslip.Micro- sem observation: micro- using same frame
Mirror, 10 × object lens of amplification factor eyepiece 10 record each visual field pollen grain number with 9 visuals field of mechanically-propelled device position observation.
Pollen number is the average value of 9 visual field pollen grain observed values of pollen, and pollen quality is the reality of alleged pollen when configuring suspension
Border quality.
2, protein content uses micro-Kjeldahl.
3, amino acid content automatic amino acid analyzer.
4, total starches measurement uses anthrone colorimetry.
5, Determination of Total Flavonoids uses liquid chromatography.
The results show that the sporoderm-broken rate of the application method is very high, at least 94%.It is compared with common peony pollen, pollen broken wall
Afterwards, crude protein, amino acid, total starches, general flavone have obtained conspicuousness raising (p < 0.05) in pollen, so peony pollen is through broken
It is more advantageous to the release of nutritional ingredient and active constituent after wall, improves its nutrient utilization.
Two, we also measured were the difference of the various albumen extracted with peony pollen.
Table 2: each protein content difference that peony pollen extracts
| Sample | Aqueous soluble protein g | Salting-in-protein g | Alcohol soluble protein g | Alkali-soluble protein g |
| Embodiment 5 | 0.3813 | |||
| Embodiment 6 | 0.3789 | |||
| Embodiment 7 | 0.0716 | |||
| Embodiment 8 | 0.0684 | |||
| Embodiment 9 | 0.3510 | |||
| Embodiment 10 | 0.3541 | |||
| Embodiment 11 | 1.1819 | |||
| Embodiment 12 | 1.1749 | |||
| Common peony pollen (control) | 0.2980 | 0.0554 | 0.2761 | 0.9240 |
Index explanation:
1, in table the measuring method of protein content referring to first part.
2, the peony pollen of embodiment 5-12 is using the peony pollen of embodiment 1 as raw material for example, control peony pollen
5g measurement is respectively chosen with the peony pollen of embodiment 1.
The results show that being compared with the peony pollen of non-broken wall, the molten egg of tree peony aqueous soluble protein, salt in the peony pollen after broken wall
White, alcohol soluble protein, alkali-soluble protein have all obtained conspicuousness raising (p < 0.05).So peony pulverizes with pollen broken wall more
The release for being conducive to nutritional ingredient and active constituent improves its nutrient utilization.
Claims (17)
1. a kind of preparation method of peony pollen polypeptide, which is characterized in that using dry peony pollen as raw material prepare and
At, the method includes peony pollen by pollen broken wall, crude protein extraction, vacuum drying, is obtained peony pollen albumen, and
Peony pollen albumen is further passed through into proteolysis, debitterizing and decoloring, centrifugation or filtering, obtains peony pollen polypeptide, wherein institute
The pollen broken wall stated is ultramicronising broken wall or whirlwind comminuting method broken wall, and the ultramicronising broken wall is peony pollen planet
Ball mill ultramicronising broken wall, revolving speed 400-600r/min, grinding time 0.5-1.5h, ratio of grinding media to material 6.8-7；The whirlwind
Comminuting method broken wall is that peony pollen is put into cyclone type high speed sample pulverizer and is crushed 1-5min using whirlwind comminuting method,
Wherein, it includes (1) aqueous soluble protein extraction step that the crude protein, which extracts, and the aqueous soluble protein extraction step, being will be after broken wall
Pollen, distilled water, 4-60 DEG C of stirring extraction 1-8h is added by material-water ratio 1:10-20, and be centrifuged 10- through 4000-8000r/min
Supernatant is collected after 20min, residue is spare, and a certain amount of solid ammonium sulfate is added step by step in supernatant, is saturated ammonium sulfate
Degree successively reaches 40%, 60%, 80%, and 1h is sufficiently stirred every time, and after 4000-8000r/min is centrifuged 10-20min, it is at different levels heavy to collect
It forms sediment, is dissolved with the phosphate buffer of pH5.0-7.0, then carry out ultrafiltration with the ultrafiltration membrane that trapped molecular weight is 100KDa,
Obtain water-soluble crude protein solution；(2) salting-in-protein extracts, and is the residue after above-mentioned extraction with aqueous solution, by solid-liquid ratio 1:
0.15-0.50mol/L sodium chloride solution is added in 10-20, and 1-8h is extracted in 4-60 DEG C of stirring, and is centrifuged through 4000-8000r/min
Supernatant is collected after 10-20min, residue is spare, and a certain amount of solid ammonium sulfate is added in supernatant, makes ammonium sulfate saturation degree
Reach 40%, 1h is sufficiently stirred, after 4000-8000r/min is centrifuged 10-20min, precipitating is collected, with the chlorination of above-mentioned comparable sodium
Sodium solution dissolution, then carries out ultrafiltration with the ultrafiltration membrane that trapped molecular weight is 100KDa, obtains the molten crude protein solution of salt；(3)
Alcohol soluble protein extracts, and is the residue after extracting above-mentioned sodium chloride solution, and it is molten that 75% ethyl alcohol is added by solid-liquid ratio 1:10-20
Liquid, 1-8h is extracted in 4-60 DEG C of stirring, and collects supernatant after 4000-8000r/min is centrifuged 10-20min, and residue is spare,
Distilled water is added by material-water ratio 1:20-30 in supernatant, 1h is sufficiently stirred, after 4000-8000r/min is centrifuged 10-20min,
Precipitating is collected, is dissolved with 75% ethyl alcohol, with after distilled water dialysis for 24 hours at 4 DEG C, the molten crude protein of alcohol is obtained and precipitates；(4) alkali-soluble protein
It extracts, is the residue after extracting above-mentioned ethanol solution, 0.10-0.75mol/L NaOH is added by solid-liquid ratio 1:10-20
Solution, 1-8h is extracted in 4-60 DEG C of stirring, and collects supernatant after 4000-8000r/min is centrifuged 10-20min, in supernatant
A certain amount of solid ammonium sulfate is added, ammonium sulfate saturation degree is made to reach 40%, 1h is sufficiently stirred, 4000-8000r/min is centrifuged 10-
After 20min, precipitating is collected, is dissolved with the NaOH of above-mentioned comparable sodium, the ultrafiltration membrane for being then 100KDa with trapped molecular weight
Ultrafiltration is carried out, alkali soluble crude protein solution is obtained；It is wherein dried in vacuo, is above-mentioned water-soluble crude protein solution, the molten crude protein of salt
The molten crude protein precipitating of solution, alcohol, alkali soluble crude protein solution, vacuum freeze drying obtains at a temperature of -55 DEG C ~ -40 DEG C respectively
Peony pollen aqueous soluble protein, salting-in-protein, alcohol soluble protein, alkali-soluble protein；The peony pollen albumen is the water-soluble egg of peony pollen
Four kinds of white, peony pollen salting-in-protein, peony pollen alcohol soluble protein, peony pollen alkali-soluble protein albumen mixing.
2. preparation method as described in claim 1, which is characterized in that the peony pollen derives from phoenix pellet tree peony or purple plague purpura
Tree peony.
3. preparation method as described in claim 1, which is characterized in that the method further includes ultrafiltration membrane after centrifugation or filtering
The step of refining.
4. preparation method as claimed in claim 3, which is characterized in that the method further includes dry, sterilizing after refining
Step.
5. the preparation method as described in claim 1-4 is any, which is characterized in that the protease be selected from papain,
One of flavor protease, alkali protease, neutral proteinase are several.
6. preparation method as claimed in claim 5, which is characterized in that the protease is alkali protease or flavor
The combination of protease and the neutral proteinase perhaps combination of alkali protease and neutral proteinase or alkali protease and wood
The combination of melon protease.
7. the preparation method as described in claim 3-4 is any, which is characterized in that the ultrafiltration membrane refinement step be will be centrifuged or
The peony pollen liquid polypeptide that person is obtained by filtration carries out classification separation by molecular weight with ultrafiltration membrane, obtains different molecular weight peony
Powder polypeptide solution, molecular cut off are 10KDa or less.
8. preparation method as claimed in claim 7, which is characterized in that molecular cut off is 5KDa or less.
9. preparation method as claimed in claim 8, which is characterized in that molecular cut off is 3KDa or less.
10. preparation method as claimed in claim 9, which is characterized in that molecular cut off is 1 KDa or less.
11. the peony pollen polypeptide of claim 1-10 either method preparation.
12. a kind of peony pollen protein polypeptide preparation, which is characterized in that the polypeptide as described in claim 11 is directly prepared
It forms.
13. a kind of peony pollen protein polypeptide preparation, which is characterized in that the polypeptide as described in claim 11 further adds
Work is at particle, tablet, capsule, drink form.
14. peony pollen polypeptide or claim 12 described in claim 11 or the 13 peony pollen polypeptide systems
The application of agent, which is characterized in that the application includes being used as nutraceutical, health food or arsenic.
15. application as claimed in claim 14, which is characterized in that the nutraceutical is the battalion provided for patient or baby
Support food；The health food is the health food provided for the elderly or sub-health population.
16. the application as described in claims 14 or 15, which is characterized in that the nutraceutical is protein beverage.
17. application as claimed in claim 16, which is characterized in that the protein beverage refers to the independent Instant Drinks of tree peony polypeptide
Or companion's Instant Drinks as existing beverage.
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| CN107432480A (en) * | 2017-08-24 | 2017-12-05 | 武汉跃莱健康产业有限公司 | The health food of strengthen immunity |
| CN107523599A (en) * | 2017-09-01 | 2017-12-29 | 兰溪市沉默生物科技有限公司 | The method that fermentation method prepares peony pollen polypeptide |
| CN107540734A (en) * | 2017-09-01 | 2018-01-05 | 磐安县派普特生物科技有限公司 | Peony pollen polypeptide prepared by fermentation method |
| CN108936625A (en) * | 2018-07-31 | 2018-12-07 | 上品（洛阳）牡丹产业有限公司 | A kind of peony bud lozenge and preparation method thereof |
| CN112841639A (en) * | 2020-11-27 | 2021-05-28 | 张贵宾 | Preparation method of peony pollen collagen peptide |
| CN113249421B (en) * | 2021-04-27 | 2023-03-03 | 天津科技大学 | Golden-silk-royal chrysanthemum protein polypeptide and preparation and application thereof |
| CN113599324A (en) * | 2021-08-19 | 2021-11-05 | 河南瑞珀斯生物科技有限公司 | Peony essence and exosome extraction method |
| CN114058665A (en) * | 2021-12-02 | 2022-02-18 | 浙江宜格企业管理集团有限公司 | Preparation method of composite flower peptide and application of composite flower peptide in preparation of cosmetics |
| CN113999885A (en) * | 2021-12-02 | 2022-02-01 | 河南科技大学 | Preparation method of peony stamen protein powder |
| CN114410724B (en) * | 2022-03-02 | 2023-09-15 | 厦门元之道生物科技有限公司 | Paeonia ostii peptide with tyrosinase activity inhibiting function and preparation method and application thereof |
| CN115386611A (en) * | 2022-10-31 | 2022-11-25 | 山东睿鹰制药集团有限公司 | Peony pollen protein polypeptide and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102659917A (en) * | 2012-05-24 | 2012-09-12 | 烟台新时代健康产业有限公司 | Method for separating and preparing crude protein and enzymolysis peptide from pollen pini |
| CN103937865A (en) * | 2014-04-28 | 2014-07-23 | 李�杰 | Peony polypeptide as well as preparation method and application thereof |
-
2014
- 2014-08-11 CN CN201410399776.5A patent/CN104152521B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102659917A (en) * | 2012-05-24 | 2012-09-12 | 烟台新时代健康产业有限公司 | Method for separating and preparing crude protein and enzymolysis peptide from pollen pini |
| CN103937865A (en) * | 2014-04-28 | 2014-07-23 | 李�杰 | Peony polypeptide as well as preparation method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 不同类型牡丹花的营养成分及体外抗氧化活性分析;史国安，郭香凤，包满珠;《农业机械学报》;20060830;第37卷(第8期);111-114 |
| 油菜花粉谷蛋白酶解肽的制备及其抗肿瘤活性研究;胡筱波;《中国博士学位论文医药卫生科技辑》;20110331;E079-14 |
| 油菜蜂花粉蛋白的分离提取及其抗氧化活性;邓建军;《食品与发酵工业》;20111031;第37卷(第10期);218-222 |
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