CN107540734A - Peony pollen polypeptide prepared by fermentation method - Google Patents
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- CN107540734A CN107540734A CN201710779680.5A CN201710779680A CN107540734A CN 107540734 A CN107540734 A CN 107540734A CN 201710779680 A CN201710779680 A CN 201710779680A CN 107540734 A CN107540734 A CN 107540734A
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Abstract
The invention discloses peony pollen polypeptide prepared by a kind of fermentation method, possesses effect of weight reducing, and there is excellent blood pressure reduction effect.The method that fermentation method prepares peony pollen polypeptide, including pollen broken wall, extraction of essential oil, fermentation, degreasing, spray drying.Peony pollen is freezed, mechanical crushing, broken wall is continued using Lactobacillus plantarum and saccharomyces cerevisiae anaerobic fermentation again, fermentation of bacillus subtilis is used again, peony pollen albumen is digested using neutral proteinase caused by its fermentation, the peony pollen polypeptide of activity intensity is obtained, degreasing is then carried out to zymotic fluid using chitosan-immobilized lipase.
Description
Technical field
The present invention relates to biological activity protein peptide art, peony pollen albumen prepared by specifically a kind of fermentation method is more
Peptide.
Background technology
Tree peony（Paeonia suffruticosa Andr.）Belong to Ranunculaceae Paeonia, machaka, there is very high sight
Reward value and pharmaceutical value.For a long time, the utilization of tree peony deep processed product but seem serious hysteresis that production added value is very
It is low, do not form industrial chain.Peony pollen is not only nutritious but also contains various bioactivators.Amino acid content exists
More than 25.0%, rich in there is 17 kinds of amino acid, vitamin B, Vitamin C content enrich, also containing tree peony polysaccharide, tree peony flavones etc.
Bioactive substance.Protein content 39.3%, far above the protein content of general pollen, protein compression is referred to as by academia
The hat of contracting body, native protein.
Natural pollen has tough and tensile cell membrane, the sporopollenin in its structure have acidproof, alkaline-resisting, heatproof, it is pressure-resistant and
The physicochemical property highly stable to hydrochloric acid in gastric juice and other digestive system enzymes, hinders human body and pollen protein is absorbed.
Prior art such as Authorization Notice No. is CN103053893B Chinese invention patent, discloses a kind of natural pollen hair
Ferment product production technology, advanced air force crushing technology, biological fermentation equipment and technology are make use of, using natural pollen as original
Material, crushes broken wall, then access prebiotic fermentative microorganism and pollen protein is degraded into amino first with air force by natural pollen
Acid and polypeptide, so as to obtain natural pollen tunnings a kind of nutritious and containing a large amount of prebiotic substances, finally through overdrying
Product is made in dry and crushing.The said goods delicate mouthfeel, it is sour-sweet it is moderate, without bitter taste stink, but the recovery rate of tree peony polypeptide
It is not high, and the activity of the functional polypeptide extracted is not strong, application prospect is limited.
The content of the invention
It is an object of the invention to provide peony pollen polypeptide prepared by a kind of fermentation method, there is excellent hypotensive
Effect, and possess effect of weight reducing.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：Peony pollen prepared by fermentation method
Polypeptide, possess effect of weight reducing, and there is excellent blood pressure reduction effect.
The method that fermentation method prepares peony pollen polypeptide, including pollen broken wall, extraction of essential oil, fermentation, degreasing, spraying
Dry.The peony pollen polypeptide being prepared will not be hydrolyzed by erepsin, directly act on internal acceptor, can promote pancreas
The duration of runs of the secretion and extension food of island element and hyperglycemic factor in enteron aisle.Meanwhile it can suppress angiotensins and turn
Change enzyme（ACE）Activity, make kallikrein kinin system（KKS）It is active, producing has the effect that reduces blood pressure
Bradykinin, blood pressure lowering effect are notable, hence it is evident that better than prior art.At the same time, peony pollen polypeptide is also for expectation
There is effect of weight reducing.
Fermentation step is：After broken wall in peony pollen add 1 ~ 6 times of volume clear water, add the essential oils of 2 ~ 5wt ‰,
0.1 ~ 0.2 wt ‰ surfactant, the Lactobacillus plantarums of 1 ~ 4 wt ‰ and the saccharomyces cerevisiaes of 1 ~ 4 wt ‰ are inoculated with after sterilizing, in 32 ~
43 DEG C, 20 ~ 30r/min of stir speed (S.S.), constant-temperatureanaerobic anaerobic is fermented 24 ~ 72h under the conditions of natural pH, 0.5 ~ 2.0 wt ‰ is inoculated with after sterilizing
Bacillus subtilis, fermented 12 ~ 24h under the conditions of mutually synthermal, stir speed (S.S.), pH, and filtrated air is passed through during fermentation
0.001~0.01m3/ min, filter to take zymotic fluid, number of repetition 1 ~ 4 time.Wherein, Lactobacillus plantarum is purchased from Shandong sunflower life
Thing Science and Technology Ltd.；Saccharomyces cerevisiae is purchased from Angel Yeast Co., Ltd；Bacillus subtilis is purchased from the high dragon biology in Shandong
Science and Technology Ltd..Only exposore is destroyed by way of freezing and mechanical breaking-wall method, sporoderm-broken rate is not high, can directly result in
The recovery rate of peony pollen polypeptide is not high.Under peony essential oil existence condition, Lactobacillus plantarum and saccharomyces cerevisiae collaboration hair
Ferment, destroy the sporopollenin of peony pollen tapetum so that the active material outflow in pollen cell.Fermentation of bacillus subtilis mistake
Journey produces neutral proteinase, the peony pollen polypeptide by the peony pollen proteolysis of dissolution into relatively small molecular weight.
Pollen broken wall step is that peony pollen is placed in into 2 ~ 24h of freezing under -40 ~ -50 DEG C of environment, mechanical crushing broken wall, is obtained
Peony pollen after to broken wall.Exposore is tentatively destroyed using mechanical crushing method, is advantageous to postorder peony pollen
Fermentation operation.
Extraction of essential oil step is：Solid-liquid ratio 1g is pressed in peony petal powder：2 ~ 6 mL add absolute ethyl alcohol, are placed in microwave
Extracted in abstraction instrument, microwave power is 200 ~ 400W, and a microwave action time is 15 ~ 35s, and extraction time is 2 ~ 6 times, filtering
Take extract to stand, remove water layer after its layering is stable, add anhydrous sodium sulfate and stand overnight, filter to take filtrate, and rotate
Obtain peony essential oil.Compared to traditional SDE methods, the essential oil yield of the method extraction of said extracted peony essential oil is higher, extraction
Temperature is relatively low, and the active ingredient in essential oil will not be made to be thermally decomposed or Oxidative demage.
Defatting step is that 2 ~ 4 ‰ chitosan-immobilized lipase are added in zymotic fluid, and constant temperature digests at 25 ~ 50 DEG C
0.8 ~ 2.5h, enzymolysis terminate rear enzyme deactivation, are filtrated to get degreasing peony pollen polypeptide liquid.Lipase can be by the fat in zymotic fluid
Fat is progressively hydrolyzed into glycerine and aliphatic acid.Lipase after immobilization, its stability, enzymatic activity, heat resistance are significantly improved,
It is and reusable.
The preparation process of chitosan-immobilized lipase is：With drying Chitosan powder, with the 60 of pH6.0 ~ 6.8 ~
80mmol/L phosphate buffer soaked overnights, then add the 1- ethyls -3- of Chitosan powder quality 0.7 ~ 1.2%（3- first
Base amino）Propyl group 0.5 ~ 0.9h of carbodiimide activation, add the glutaraldehyde coupling reaction 1 of 1 ~ 2 times of volume of Chitosan powder ~
1.8h, cleaned 5 ~ 8 times with distilled water, 0.7 ~ 2.0mg/mL lipase solutions and 0.1 ~ 0.3 ‰ bioactive peptides are added, in ice bath
0.5 ~ 1.5h is slowly stirred, then places refrigerator overnight, secondary daily distilled water cleaning down, filtration drying obtains chitosan
Immobilized lipase.1- ethyls -3-（3- methylaminos）Propyl group carbodiimide carries out chemical modification, effective activation to chitosan
Chitosan surface hydroxyl groups.Chitosan surface amino groups after activation are coupled to obtain excellent lipase immobilization load with glutaraldehyde
Body.Lipase adsorption forms chitosan-immobilized lipase on carrier.
The amino acid sequence of bioactive peptide is SCASVCKARCLRARGCRCVSVYCRCLR.Above-mentioned bioactive peptide can be obvious
Strengthen the physics and chemisorption power between lipase and lipase immobilization carrier, improve immobilized lipase enzymatic conversion
Rate, and improve the reusable number of chitosan-immobilized lipase.
Surfactant is food grade surfactant.
Compared with prior art, the advantage of the invention is that：
1. the peony pollen polypeptide being prepared will not be hydrolyzed by erepsin, internal acceptor is directly acted on, can be promoted
The duration of runs of the secretion and extension food of insulin and hyperglycemic factor in enteron aisle.Meanwhile it can suppress angiotensins
Converting Enzyme（ACE）Activity, make kallikrein kinin system（KKS）It is active, generation has the effect of reducing blood pressure
Bradykinin, blood pressure lowering effect is notable, hence it is evident that better than prior art.At the same time, peony pollen polypeptide is also for meaning
Material has effect of weight reducing；
2. using 1- ethyls -3-（3- methylaminos）Propyl group carbodiimide activation chitosan, and be coupled to form fat with glutaraldehyde
Enzyme immobilization carrier, the bioactive peptide that amino acid sequence is SCASVCKARCLRARGCRCVSVYCRCLR is recycled to be carried to improve
Physics and chemisorbed power between body and lipase, improve stability, enzymatic activity, heat resistance and the access times of lipase；
3. under peony essential oil existence condition, Lactobacillus plantarum and saccharomyces cerevisiae cooperative fermentation, peony pollen tapetum is destroyed
Sporopollenin so that the active material outflow in pollen cell.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
Peony pollen polypeptide prepared by fermentation method, possesses effect of weight reducing, and have excellent blood pressure reduction effect.
The method that fermentation method prepares peony pollen polypeptide, comprises the following steps：
1）Pollen broken wall：Peony pollen is placed in 2 ~ 24h of freezing, mechanical crushing broken wall, after obtaining broken wall under -40 ~ -50 DEG C of environment
Peony pollen；
2）Extraction of essential oil：Solid-liquid ratio 1g is pressed in peony petal powder：2 ~ 6 mL add absolute ethyl alcohol, are placed in Microwave Extraction Apparatus
Extraction, microwave power are 200 ~ 400W, and a microwave action time is 15 ~ 35s, and extraction time is 2 ~ 6 times, filters to take extract
Stand, remove water layer after its layering is stable, add anhydrous sodium sulfate and stand overnight, filter to take filtrate, and rotate and obtain tree peony
Essential oil；
3）Fermentation：The clear water of 1 ~ 6 times of volume is added in peony pollen after broken wall, adds the essential oils of 2 ~ 5wt ‰, 0.1 ~ 0.2
Wt ‰ surfactant, the Lactobacillus plantarums of 1 ~ 4 wt ‰ and the saccharomyces cerevisiaes of 1 ~ 4 wt ‰ are inoculated with after sterilizing, in 32 ~ 43 DEG C, stir
Mix constant-temperatureanaerobic anaerobic under the conditions of 20 ~ 30r/min of speed, natural pH to ferment 24 ~ 72h, the withered grass buds of 0.5 ~ 2.0 wt ‰ are inoculated with after sterilizing
Spore bacillus, under the conditions of mutually synthermal, stir speed (S.S.), pH ferment 12 ~ 24h, be passed through during fermentation filtrated air 0.001~
0.01m3/ min, filter to take zymotic fluid, number of repetition 1 ~ 4 time；
4）Degreasing：2 ~ 4 ‰ chitosan-immobilized lipase are added in zymotic fluid, constant temperature digests 0.8 ~ 2.5h at 25 ~ 50 DEG C,
Enzymolysis terminates rear enzyme deactivation, is filtrated to get degreasing peony pollen polypeptide liquid.The preparation process of chitosan-immobilized lipase is：
With Chitosan powder is dried, with 60 ~ 80mmol/L phosphate buffer soaked overnights of pH6.0 ~ 6.8, chitosan is then added
1- ethyls-the 3- of powder quality 0.7 ~ 1.2%（3- methylaminos）Propyl group 0.5 ~ 0.9h of carbodiimide activation, adds chitosan
Glutaraldehyde 1 ~ the 1.8h of coupling reaction of 1 ~ 2 times of volume of powder, is cleaned 5 ~ 8 times with distilled water, adds 0.7 ~ 2.0mg/mL lipase
Solution and 0.1 ~ 0.3 ‰ bioactive peptides, are slowly stirred 0.5 ~ 1.5h in ice bath, then place refrigerator overnight, secondary daily steaming
Distilled water cleaning down, filtration drying obtain chitosan-immobilized lipase.The amino acid sequence of bioactive peptide is
SCASVCKARCLRARGCRCVSVYCRCLR；
5）Spray drying：Fermented liquid spray drying after degreasing is obtained into peony pollen polypeptide powder.Above-mentioned peony pollen
Polypeptide will not be hydrolyzed by erepsin, directly act on internal acceptor, can promote the secretion of insulin and hyperglycemic factor
With the duration of runs of the extension food in enteron aisle.Meanwhile it can suppress Angiotensin-Converting（ACE）Activity, make kassinin kinin
Discharge enzyme-kinin system（KKS）It is active, produces the bradykinin with the effect that reduces blood pressure, blood pressure lowering effect shows
Write, hence it is evident that better than prior art.At the same time, peony pollen polypeptide also has effect of weight reducing for expectation.
Embodiment 2：
Peony pollen polypeptide prepared by fermentation method, possesses effect of weight reducing, and have excellent blood pressure reduction effect.
Fermentation method prepares the most preferred method of peony pollen polypeptide, comprises the following steps：
1）Pollen broken wall：Peony pollen is placed under -45 DEG C of environment and freezes 12h, mechanical crushing broken wall, obtains peony after broken wall
Powder；
2）Extraction of essential oil：Solid-liquid ratio 1g is pressed in peony petal powder：4mL adds absolute ethyl alcohol, is placed in Microwave Extraction Apparatus and extracts
Take, microwave power 300W, a microwave action time is 25s, and extraction time is 3 times, filters to take extract standing, treats its point
Remove water layer after layer is stable, add anhydrous sodium sulfate and stand overnight, filter to take filtrate, and rotate and obtain peony essential oil；
3）Fermentation：The clear water of 3 times of volumes is added in peony pollen after broken wall, adds the essential oils of 4wt ‰, 0.2 wt ‰ is told
Temperature -80, the Lactobacillus plantarums of 3 wt ‰ and the saccharomyces cerevisiaes of 2wt ‰ are inoculated with after sterilizing, in 35 DEG C, stir speed (S.S.) 20r/min, natural pH
Under the conditions of constant-temperatureanaerobic anaerobic fermentation 36h, the bacillus subtilises of 1.0 wt ‰ are inoculated with after sterilizing, in mutually synthermal, stir speed (S.S.), pH bars
Fermented 24h under part, and filtrated air 0.008m is passed through during fermentation3/ min, filter to take zymotic fluid, number of repetition 3 times；
4）Degreasing：3 ‰ chitosan-immobilized lipase are added in zymotic fluid, constant temperature digests 1.5h at 32 DEG C, and enzymolysis terminates
Enzyme deactivation afterwards, it is filtrated to get degreasing peony pollen polypeptide liquid.The preparation process of chitosan-immobilized lipase is：With dry shell
Glycan powder, with pH6.5 70mmol/L phosphate buffer soaked overnights, then add Chitosan powder quality 1.0%
1- ethyls -3-（3- methylaminos）Propyl group carbodiimide activation 0.7h, the glutaraldehyde for adding 1.0 times of volumes of Chitosan powder are even
Connection reaction 1.2h, is cleaned 8 times with distilled water, adds 1.2mg/mL lipase solutions and 0.3 ‰ bioactive peptides, in ice bath slowly
0.9h is stirred, then places refrigerator overnight, secondary daily distilled water cleaning down, filtration drying obtains chitosan-immobilized fat
Fat enzyme.The amino acid sequence of bioactive peptide is SCASVCKARCLRARGCRCVSVYCRCLR；
5）Spray drying：Fermented liquid spray drying after degreasing is obtained into peony pollen polypeptide powder.Above-mentioned peony pollen
Polypeptide will not be hydrolyzed by erepsin, directly act on internal acceptor, can promote the secretion of insulin and hyperglycemic factor
With the duration of runs of the extension food in enteron aisle.Meanwhile it can suppress Angiotensin-Converting（ACE）Activity, make kassinin kinin
Discharge enzyme-kinin system（KKS）It is active, produces the bradykinin with the effect that reduces blood pressure, blood pressure lowering effect shows
Write, hence it is evident that better than prior art.At the same time, peony pollen polypeptide also has effect of weight reducing for expectation.
Embodiment 3：
Peony pollen polypeptide prepared by fermentation method, possesses effect of weight reducing, and have excellent blood pressure reduction effect.
The method that fermentation method prepares peony pollen polypeptide, comprises the following steps：
1）Pollen broken wall：Peony pollen is placed under -50 DEG C of environment and freezes 12h, mechanical crushing broken wall, obtains peony after broken wall
Powder；
2）Extraction of essential oil：Solid-liquid ratio 1g is pressed in peony petal powder：6mL adds absolute ethyl alcohol, is placed in Microwave Extraction Apparatus and extracts
Take, microwave power 400W, a microwave action time is 15s, and extraction time is 3 times, filters to take extract standing, treats its point
Remove water layer after layer is stable, add anhydrous sodium sulfate and stand overnight, filter to take filtrate, and rotate and obtain peony essential oil；
3）Fermentation：The clear water of 3 times of volumes is added in peony pollen after broken wall, adds the essential oils of 5wt ‰, 0.2 wt ‰ is told
Temperature -20, the Lactobacillus plantarums of 4 wt ‰ and the saccharomyces cerevisiaes of 4 wt ‰ are inoculated with after sterilizing, in 35 DEG C, stir speed (S.S.) 30r/min, nature
Constant-temperatureanaerobic anaerobic fermentation 24h, is inoculated with the bacillus subtilises of 2.0 wt ‰, in mutually synthermal, stir speed (S.S.), pH under the conditions of pH after sterilizing
Under the conditions of ferment 12h, filtrated air 0.01m is passed through during fermentation3/ min, filter to take zymotic fluid, number of repetition 2 times；
4）Degreasing：4 ‰ chitosan-immobilized lipase are added in zymotic fluid, constant temperature digests 1.5h at 28 DEG C, and enzymolysis terminates
Enzyme deactivation afterwards, it is filtrated to get degreasing peony pollen polypeptide liquid.The preparation process of chitosan-immobilized lipase is：With dry shell
Glycan powder, with pH6.5 80mmol/L phosphate buffer soaked overnights, then add Chitosan powder quality 1.2%
1- ethyls -3-（3- methylaminos）Propyl group carbodiimide activation 0.7h, add the glutaraldehyde coupling of 2 times of volumes of Chitosan powder
1.8h is reacted, is cleaned 8 times with distilled water, 2.0mg/mL lipase solutions and 0.3 ‰ bioactive peptides is added, is slowly stirred in ice bath
0.5h is mixed, then places refrigerator overnight, secondary daily distilled water cleaning down, filtration drying obtains chitosan-immobilized fat
Enzyme.The amino acid sequence of bioactive peptide is SCASVCKARCLRARGCRCVSVYCRCLR；
5）Spray drying：Fermented liquid spray drying after degreasing is obtained into peony pollen polypeptide powder.Above-mentioned peony pollen
Polypeptide will not be hydrolyzed by erepsin, directly act on internal acceptor, can promote the secretion of insulin and hyperglycemic factor
With the duration of runs of the extension food in enteron aisle.Meanwhile it can suppress Angiotensin-Converting（ACE）Activity, make kassinin kinin
Discharge enzyme-kinin system（KKS）It is active, produces the bradykinin with the effect that reduces blood pressure, blood pressure lowering effect shows
Write, hence it is evident that better than prior art.At the same time, peony pollen polypeptide also has effect of weight reducing for expectation.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
Sequence table
<110>Send Pood bio tech ltd in Panan County
<120>Peony pollen polypeptide prepared by fermentation method
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 27
<212> PRT
<213>Artificial synthesized (Mytilus coruscus)
<400> 1
Ser Cys Ala Ser Val Cys Lys Ala Arg Cys Leu Arg Ala Arg Gly Cys
1 5 10 15
Arg Cys Val Ser Val Tyr Cys Arg Cys Leu Arg
20 25
Claims (7)
1. peony pollen polypeptide prepared by fermentation method, it is characterised in that：Described peony pollen polypeptide possesses fat-reducing
Effect, and there is excellent blood pressure reduction effect.
2. peony pollen polypeptide prepared by fermentation method according to claim 1, it is characterised in that：Described peony
The preparation method of amyloid proteins polypeptide includes pollen broken wall, extraction of essential oil, fermentation, degreasing, spray drying；Wherein, described fermentation
Step is：The clear water of 1 ~ 6 times of volume is added in peony pollen after broken wall, adds the essential oils of 2 ~ 5wt ‰, 0.1 ~ 0.2 wt ‰
Surfactant, the Lactobacillus plantarums of 1 ~ 4 wt ‰ and the saccharomyces cerevisiaes of 1 ~ 4 wt ‰ are inoculated with after sterilizing, in 32 ~ 43 DEG C, stir speed (S.S.)
24 ~ 72h of constant-temperatureanaerobic anaerobic fermentation, is inoculated with the bacillus subtilises of 0.5 ~ 2.0 wt ‰ under the conditions of 20 ~ 30r/min, natural pH after sterilizing,
Fermented 12 ~ 24h under the conditions of mutually synthermal, stir speed (S.S.), pH, and 0.001~0.01m of filtrated air is passed through during fermentation3 /
Min, filter to take zymotic fluid, number of repetition 1 ~ 4 time.
3. peony pollen polypeptide prepared by fermentation method according to claim 2, it is characterised in that：Described pollen is broken
Wall step is that peony pollen is placed in into 2 ~ 24h of freezing under -40 ~ -50 DEG C of environment, mechanical crushing broken wall, obtains peony after broken wall
Powder.
4. peony pollen polypeptide prepared by fermentation method according to claim 2, it is characterised in that：Described essential oil carries
The step is taken to be：Solid-liquid ratio 1g is pressed in peony petal powder：2 ~ 6 mL add absolute ethyl alcohol, are placed in Microwave Extraction Apparatus and extract,
Microwave power is 200 ~ 400W, and a microwave action time is 15 ~ 35s, and extraction time is 2 ~ 6 times, filters to take extract standing,
Remove water layer after its layering is stable, add anhydrous sodium sulfate and stand overnight, filter to take filtrate, and rotate and obtain peony essential oil.
5. peony pollen polypeptide prepared by fermentation method according to claim 2, it is characterised in that：Described degreasing step
Rapid is that 2 ~ 4 ‰ chitosan-immobilized lipase are added in zymotic fluid, and constant temperature digests 0.8 ~ 2.5h, enzymolysis knot at 25 ~ 50 DEG C
Enzyme deactivation after beam, it is filtrated to get degreasing peony pollen polypeptide liquid.
6. peony pollen polypeptide prepared by fermentation method according to claim 5, it is characterised in that：Described chitosan
The preparation process of immobilized lipase is：With Chitosan powder is dried, with 60 ~ 80mmol/L phosphate-buffereds of pH6.0 ~ 6.8
Liquid soaked overnight, then add the 1- ethyls -3- of Chitosan powder quality 0.7 ~ 1.2%（3- methylaminos）Propyl group carbodiimide
0.5 ~ 0.9h is activated, the glutaraldehyde 1 ~ 1.8h of coupling reaction of 1 ~ 2 times of volume of Chitosan powder is added, 5 ~ 8 is cleaned with distilled water
It is secondary, 0.7 ~ 2.0mg/mL lipase solutions and 0.1 ~ 0.3 ‰ bioactive peptides are added, 0.5 ~ 1.5h are slowly stirred in ice bath, so
After place refrigerator overnight, secondary daily distilled water cleaning down, filtration drying obtains chitosan-immobilized lipase.
7. peony pollen polypeptide prepared by fermentation method according to claim 6, it is characterised in that：Described is active short
The amino acid sequence of peptide is SCASVCKARCLRARGCRCVSVYCRCLR.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110050977A (en) * | 2019-05-28 | 2019-07-26 | 西北农林科技大学 | A kind of peony pollen electuary and preparation method thereof |
| CN114058665A (en) * | 2021-12-02 | 2022-02-18 | 浙江宜格企业管理集团有限公司 | Preparation method of composite flower peptide and application of composite flower peptide in preparation of cosmetics |
| CN114410724A (en) * | 2022-03-02 | 2022-04-29 | 厦门元之道生物科技有限公司 | Paeonia ostii peptide with tyrosinase activity inhibition function and preparation method and application thereof |
| CN115386611A (en) * | 2022-10-31 | 2022-11-25 | 山东睿鹰制药集团有限公司 | Peony pollen protein polypeptide and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104152521A (en) * | 2014-08-11 | 2014-11-19 | 李�杰 | Paeonia suffruticosa pollen protein polypeptide and preparation method and application thereof |
| CN105707802A (en) * | 2016-03-25 | 2016-06-29 | 北京科慧通智慧科技有限公司 | Bee pollen easy to digest and preparation method thereof |
-
2017
- 2017-09-01 CN CN201710779680.5A patent/CN107540734A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104152521A (en) * | 2014-08-11 | 2014-11-19 | 李�杰 | Paeonia suffruticosa pollen protein polypeptide and preparation method and application thereof |
| CN105707802A (en) * | 2016-03-25 | 2016-06-29 | 北京科慧通智慧科技有限公司 | Bee pollen easy to digest and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 李再新: "《制药分离工程实验》", 30 June 2016, 西南交通大学出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110050977A (en) * | 2019-05-28 | 2019-07-26 | 西北农林科技大学 | A kind of peony pollen electuary and preparation method thereof |
| CN114058665A (en) * | 2021-12-02 | 2022-02-18 | 浙江宜格企业管理集团有限公司 | Preparation method of composite flower peptide and application of composite flower peptide in preparation of cosmetics |
| CN114410724A (en) * | 2022-03-02 | 2022-04-29 | 厦门元之道生物科技有限公司 | Paeonia ostii peptide with tyrosinase activity inhibition function and preparation method and application thereof |
| CN114410724B (en) * | 2022-03-02 | 2023-09-15 | 厦门元之道生物科技有限公司 | Paeonia ostii peptide with tyrosinase activity inhibiting function and preparation method and application thereof |
| CN115386611A (en) * | 2022-10-31 | 2022-11-25 | 山东睿鹰制药集团有限公司 | Peony pollen protein polypeptide and preparation method and application thereof |
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