JP2006008645A - Method for producing extract of inonotus obliquus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for effectively extracting active constituents from e.g. sclerotia of Inonotus obliquus. <P>SOLUTION: The method comprises adding a specified amount of mineral-containing purified water to Inonotus obliquus in the form of a ground raw material, ultrasonicating the mixture, cooking the ultrasonicated mixture, separating the cooked liquid, extracting and dissociating the active ingredients from the raw material by supplying electrons to the cooked raw material in the presence of vegetable-origin quinones, flavonoids, and proteases and an organic solvent, and keeping the mixture for a specified time, and mixing the obtained extracted and dissociated liquid with the previously separated cooked liquid, and subjecting the resulting mixture to sterilization and filtration. According to this method, it is possible not only to efficiently extract and fully utilize various active constituents from Inonotus obliquus but also to obtain an extract expected to have marked effects such as antitumor activity and blood glucose lowering action. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a method for producing a birch extract, and more particularly to a method capable of efficiently extracting an active ingredient contained in a mycelium of birch.

Generally, moss is known to have various medicinal effects and is widely used not only as a food product but also as various health foods.
Among these moths, the birch (Fuscoporia obliqué (Persoon ex Fries.) Aoshima) is a cold-resistant moth belonging to the Fusarium moss family, Fuscoporia spp. It is widely distributed in cold regions such as northern Canada, northern China and Hokkaido in Japan, and parasitizes birch trees such as birch and birch.

The birch tree breaks the bark of the birch tree and forms a black and hard fungus called `` Chaga '', and the fruiting body spreads thinly and flatly under the tree without forming an umbrella. It has enough vitality to grow up and eventually kill the parasitic trees.
And in Russia's Alekandrov region, the habit of roasting Chaga (fungal nuclei) and drinking it instead of tea has been around for a long time, and β-glucan, a high-molecular polysaccharide that is abundantly contained in Chaga and mycelium. As a result of research, it has been disclosed that ingredients such as ergosterol and flavonoid, which are precursors of vitamin D2, are effective not only for antitumor activity and hypoglycemic activity but also for disease prevention, anti-aging activity, and immunity improvement. (For example, Patent Document 1).

  Japanese Patent No. 3008292

However, it is not only difficult to obtain because it grows over 10-15 years in a cold region where the temperature is below 20 ° C, but it is difficult to obtain. It was also difficult to obtain by artificial cultivation.
In addition, when using this birch salmon as a health food, it can be used by decoctioning the finely crushed Chaga as in the Russian region of Alekandrov, or processing powdered Chaga and fruit body into tablets and granules together with a binder. The method of taking it is adopted.
Furthermore, in recent years, a technique for extracting an extract from Chaga using a drug has begun to be developed, and it has been found that the active ingredient contained in the Chaga extract obtained by this method has a higher content than before. .

  By the way, moss such as birch can be extracted only about 20% of the effective components by hot water extraction (watering and heating), and enormous energy is required for pulverization and granulation Furthermore, when extracting using a chemical | medical agent, not only the manufacturing cost increased, but the problem which should be solved was pointed out, for example, it was necessary to take the means to prevent mixing of a chemical component.

  The present invention has been made in view of such problems, and an object of the present invention is to provide a method capable of efficiently extracting an active ingredient contained in fungal nuclei of birch moss. .

  That is, in the present invention, a certain amount of purified water containing minerals is added to birch salmon as a crushed raw material and subjected to sonication, and then the sonicated raw material is steamed to separate its cooking liquid, while steaming By supplying electrons in the presence of plant-derived quinones, flavonoids, proteolytic enzymes and organic solvents and holding them for a certain period of time, the active ingredients contained in the raw materials are extracted and dissociated. The extracted dissociation liquid and the previously separated cooking liquid are mixed and then sterilized and filtered.

In this case, birch salmon as a raw material uses mycorrhiza and / or fruiting bodies as desired, and as minerals, ionic solids (salts), ionized minerals, etc. may be used alone or a mixture thereof. it can.
On the other hand, quinones, flavonoids, and proteolytic enzymes are included in the genus departments such as wolfberry and scallops, the grass family such as wolffish, kumazasa, and pearl barley, the cruciferous family such as suriphne and foxfry, Using quinones, flavonoids, and proteolytic enzymes extracted from plants of at least two species of legumes such as Yamagi, Asteraceae such as Yakushi, Artemisia, and lilies such as Nobil and Katakuri Is preferably dissolved in any organic solvent such as ethanol, methanol, isopropyl alcohol, etc., ether, normal hexane, xylene, acetone, etc., but as an alternative, the quinones, flavonoids, proteins Decomposing enzyme into porous ceramic Immersed in wood may be used by introducing the ceramic material into the aforementioned organic solvents.
Moreover, when mixing the extract dissociation liquid and the steamed liquid, it is preferable to adopt a step of filtering the extract dissociation liquid in advance, mixing and sterilizing and filtering, considering the quality of the extract obtained.
The obtained birch extract is not only used as it is, but also suitably used as a powder composition prepared by mixing this birch extract with a predetermined amount of cyclic starch granules and further subjecting to dry grinding. Can do.

According to the method for producing the birch extract according to the present invention described above;
(1) Add a specified amount of purified water containing minerals to the birch as a crushed raw material, apply ultrasonic treatment, then boil the ultrasonically processed raw material to separate the cooking liquid, Extraction and dissociation of active ingredients contained in raw materials by supplying electrons in the presence of plant-derived quinones, flavonoids, proteolytic enzymes and organic solvents and holding them for a specified period In addition to efficient extraction of insoluble dietary fiber and polysaccharides, which were difficult to extract in the past, they can be used for drinking. This can dramatically increase the absorbency.
(2) Also, when the birch extract is prepared in a powder form by dispersing it in a circular starch granule, the bulk specific gravity is large, so not only can the weight be reduced but also the liquefaction can be easily handled, Since the hygroscopicity is low, the storage stability can be improved.
(3) In addition, antibacterial moss itself has various anti-tumor activity and hypoglycemic activity due to various active ingredients, and expects an ideal pharmacological effect without the side effects seen in chemotherapeutic agents. It is something that can be done.

Next, the best embodiment in the method for producing the birch extract of the present invention is illustrated in the present invention and will be described in detail below.
In FIG. 1, in the method of the present invention, firstly, a predetermined amount of Chaga (fungal nuclei) crushed as a raw material is prepared in a container 10. In this case, birch salmon as a raw material is not limited to chaga as in the present embodiment, and a child entity is added to chaga as required, or only a child entity is used depending on the intended effect. Needless to say, it can be appropriately selected.
Further, the chaga is pulverized by a mechanical process using a pulverizer or the like so as to have a particle size of about 0.1 mm to 0.3 mm, for example. And after adding the purified water 14 which added the mineral in the range of 0.2%-0.5% to the tap water from which chlorine etc. were removed in advance to the granular chaga 12 to make it wet, purified water The cells are destroyed to some extent by the treatment with the ultrasonic generator 16 so that the mineral added to 14 can easily enter the cells of the Chaga in a short time.

  In this embodiment, the mineral added to the purified water 14 is obtained by pulverizing a raw material in which starch, several kinds of plant seeds, eggshells, and the like are mixed at a predetermined ratio. Ionized mineral liquid obtained by filtering after dissociating minerals such as calcium, magnesium, sodium, potassium, iron, copper, zinc, manganese, etc. contained in the raw material by fermentation and aging In addition to this ionized mineral liquid, for example, ionic solids such as eggshell powder (calcium carbonate) and magnesium carbonate having a porous structure with a large surface area and an extremely low phosphorus content and an average particle size of 10 μm or less ( Salt), or a mixture of these at a predetermined ratio.

  The purified water 14 is further added to the chaga 18 which has been subjected to ultrasonic treatment in this manner, and the steamed liquid 20 is separated after cooking for 15 minutes by a known heating means. As mentioned above, the Chaga 18 whose cells have been destroyed to some extent by ultrasonic treatment is cooked together with the purified water 14, so that not only the raw Chaga sterilization but also the water-soluble nutrients and water-soluble dietary fiber contained in the Chaga For example, a part of a high-molecular polysaccharide including β-glucan can be bound to ionized calcium, potassium, manganese, etc. and eluted in the cooking liquid 20.

On the other hand, the Chaga 18 from which the steaming solution 20 has been separated is mixed with an organic solvent 22 containing plant-derived quinones, flavonoids, proteolytic enzymes, and the like, and if necessary, natural salt in an amount of 0.05% to 0. Add about 07%.
In this case, quinones, flavonoids, and proteolytic enzymes are included in the genus family such as wolfberry and scallops, the grass family such as okameasa, kumazasa, and pearl barley, the Brassicaceae family such as suriphne and foxflies, , Legumes such as Yamagi, Asteraceae such as Yakusou, Artemisia, and lily family such as Nobil, Katakuri, etc., by hot water extraction, alcohol extraction, solvent extraction, supercritical extraction, etc. Use the one obtained by the extraction method.
Further, as the organic solvent 22, alcohol such as ethanol, methanol, isopropyl alcohol, ether, normal hexane, xylene, acetone or the like can be used, but alcohol is preferable from the viewpoint of safety and cost when remaining. More preferably, 60 to 95% hydrous ethanol or distilled liquor having an alcohol content of 25 to 35 degrees is used.
In the present embodiment, alcohol as an organic solvent to which plant-derived quinones, flavonoids, proteolytic enzymes and the like are added is used, but as an alternative, plant-derived quinones, flavonoids, and proteolytic enzymes are used. The organic solvent etc. which supplied the ceramic raw material which soaked etc. can be used suitably.
Further, the amount of the organic solvent 22 added to the chaga 18 is preferably set to 5 to 20 times the amount of chaga. If the organic solvent 22 is 5 times or less, it takes a long time to obtain an active ingredient. In addition, when the amount exceeds 20 times, the amount is too large and the economic efficiency is lowered.

  Then, the chaga 18 to which a predetermined amount of the organic solvent 22 is added is held for about two months while being slowly stirred in an electron (-e) supply atmosphere while being held at 30 ° C. to 35 ° C. by a heater. Various active ingredients including macromolecular polysaccharides, dietary fibers such as hemicellulose, amino acids and the like contained in Chaga as a raw material are dissociated and eluted in the organic solvent 22.

Next, the extract dissociation liquid 24 from which various active ingredients of Chaga are eluted and the steamed liquid 20 separated in advance are mixed at a predetermined ratio, and the steamed extracted liquid mixture 26 is sterilized by means such as ultraviolet irradiation. Then, after standing for a predetermined period, it is prepared as a birch extract 28 by microfiltration.
In this case, if the birch extract 28 is prepared by preliminarily filtering and removing the precipitate or starch of the extract dissociation liquid 24 and then sterilizing and filtering the steamed extract mixed liquid 26 mixed with the steamed liquid 20, As a result, the quality of the birch extract 28 can be improved.

The birch salmon extract 28 thus obtained contains 20 or more amino acids including valine, glutamic acid, proline, leucine, etc., high molecular polysaccharides such as β-glucan, ergosterol which is a precursor of vitamin D2, sugar In addition to quality, dietary fiber, calcium, magnesium, potassium, and sodium, trace minerals such as iron and zinc are abundant. An example of the composition is as follows.
Minerals and others (per 100g) Nutrients (per 100g)
・ Calcium 201mg ・ Energy 176kcal
・ Magnesium 480mg ・ Protein 2.5g
-Sodium 4.2mg-Lipid 1.4g
・ Potassium 140mg ・ Dietary fiber 70.4g
・ Iron 4.58mg ・ Moisture 81.8g
・ Zinc 6.25μg ・ Carbohydrate 82.4g
・ Β-glucan 16.5 g ・ Ash 9.7 g

Experimental example 1
Next, the birch salmon extract (chaga extract) 28 (hereinafter referred to as the extract of the present invention) obtained by the above-described process and the granular chaga 12 prepared in the container 10 are twice the amount obtained by removing chlorine and the like in the same manner as described above. Comparison was made with respect to the absorbability of Chaga boiled liquid = control (hereinafter boiled extract) obtained by steaming for 15 minutes with tap water and filtering.

First, purified water (Japan Pharmacopoeia, manufactured by Ando Pharmaceutical Co., Ltd.) obtained by purifying clean water with an ion exchange resin, filtering, and sterilizing with ultraviolet rays (UV) was added to 200 cc ( A water temperature of 25.5 ° C. was prepared and the electrical resistance value was measured, and a value of 320.0 × 10 −3 Ω was shown. For reference, the electrical resistance values of the boiled extract and the extract of the present invention were measured and found to be 14.0 × 10 −3 Ω and 7.5 × 10 −3 Ω.
Next, each of the boiled extract and the inventive extract was placed in a container A. A predetermined amount was dripped into purified water of B (water temperature 25.5 ° C.), and after sufficiently stirring, the electric resistance value was measured.

According to this measurement result, the electrical resistance value of the purified water in the container A into which the boiled extract was dropped was compared with the electrical resistance value (320 × 10 −3 Ω) of only the purified water without dropping the boiled extract. As the value increases, the value decreases gradually from about 1 / 3.2 (0.5%) to about 1 / 6.1 (1.0%) to about 1 / 8.4 (1.5%). This indicates that the conductivity (electrical conductivity) is improved. The reason why the electrical resistance value does not decrease so much even when the drop concentration of the extract is increased is that the dispersibility in water is low although elution such as water-soluble mineral components is caused by boiling.
On the other hand, the change in the electrical resistance value in the purified water in the container B into which the extract of the present invention was dropped was about 1 / 15.6 → about 1 / 33.6 → about the electrical resistance value of the purified water alone. Not only decreases to 1 / 40.5, but all values are much smaller than the electrical resistance of container A (purified water + boiled extract). I understand that there are many. Moreover, since the dripping concentration is only 1.5%, an electric resistance value (7.9 × 10 −3 Ω) close to the electric resistance value (7.5 × 10 −3 Ω) of the extract solution of the present invention is obtained, so that It is recognized that the dispersibility is remarkably high.

  According to this experiment, although the electrical resistance value of purified water (water in container A) to which the boiled extract was dropped is lower than the electrical resistance value of purified water alone, water-soluble mineral components (for example, potassium and magnesium) contained in Chaga In contrast, purified water (water in container B) to which the extract of the present invention was dropped dropped most of various mineral components contained in Chaga, so that the electrical resistance value was small. Remarkably lowered (higher electrical conductivity) and improved absorbability to human body. Therefore, various amino acids, high-molecular polysaccharides, ergosterol, carbohydrates and dietary fiber contained in these minerals It can be sufficiently expected that the absorbability of the components will be greatly improved.

Moreover, FIG. 2 is embodiment which manufactures a powder composition using the birch salmon extract obtained by the above-mentioned method.
In other words, the birch extract 28 is mixed with starch granules 30 made into a circular shape by previously processing plant-derived starch granules such as potato, potato, etc., for example, at a weight ratio of about 1: 2.5, and then the water content Is dried at a low temperature of 50 ° C. or less until it becomes 5.0% or less, and further pulverized to a particle size of 50 μm or less to prepare a birch extract powder 32.
The birch salmon extract powder 32 obtained in this way has a large bulk specific gravity, so that it can be lightened and easily liquefied, so that it is easy to handle and has low hygroscopicity, thus improving storage stability. Therefore, it is possible to intend to expand the use of the birch extract.

It is a schematic process explanatory drawing which shows suitable embodiment of the manufacturing method of the birch salmon extract based on this invention. It is a schematic process explanatory drawing which shows suitable embodiment in the case of manufacturing a powder composition using the birch extract obtained by the method shown in FIG.

Explanation of symbols

10 ... container,
12 ... Granular Chaga 14 ... Purified water 16 ... Ultrasonic generator 18 ... Ultrasonic-treated Chaga 20 ... Steamed liquid 22 ... Organic solvent 24 ... Extracted dissociated liquid 26 ... Steamed extract mixed liquid 28 ... Birch extract
30 ... cyclic starch granules 32 ... birch extract powder

Claims (8)

Add a specified amount of purified water containing minerals to the bamboo shoots as a crushed raw material and apply sonication, then boil the sonicated raw material to separate the cooking liquid, while the boiled raw material is plant-derived Electrons are supplied in the presence of quinones, flavonoids, proteolytic enzymes and organic solvents and held for a predetermined period of time to extract and dissociate active ingredients contained in the raw material. A method for producing a birch extract, which comprises sterilizing filtration after mixing with a steamed liquor that has been separated. The method for producing a birch extract of claim 1, wherein the birch is a mycelium and / or fruiting body. The method for producing birch salmon extract according to claim 1 or 2, wherein an ionic solid or an ionized mineral is used alone or a mixture thereof as the mineral. Quinones, flavonoids, and proteolytic enzymes include scorpionaceae such as dokudami and chrysanthemum, gramineae such as okasasa, kumazasa and pearl barley; The birch extract of any one of claims 1 to 3, wherein the extract is from any two or more plants of the family Asteraceae such as legumes, yakusou and mugwort, and also lilies such as nobil and katakari. Manufacturing method. The method for producing birch salmon extract according to any one of claims 1 to 4, wherein any one of alcohols such as ethanol, methanol and isopropyl alcohol, ether, normal hexane, xylene and acetone is used as the organic solvent.   The method for producing a birch extract according to any one of claims 1 to 5, wherein, when the extract dissociation liquid and the steamed liquid are mixed, the extract dissociation liquid is previously filtered and mixed, and further sterilized and filtered. The birch extract obtained by the method of Claims 1-6. A powder composition of the birch extract obtained by mixing the birch extract obtained by the method according to claims 1 to 6 with a predetermined amount of cyclic starch granules, followed by drying and grinding.

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