CN101248890A - Nutrition reinforced type high activity beche-de-mer products and method of preparing the same - Google Patents
Nutrition reinforced type high activity beche-de-mer products and method of preparing the same Download PDFInfo
- Publication number
- CN101248890A CN101248890A CNA2008100888968A CN200810088896A CN101248890A CN 101248890 A CN101248890 A CN 101248890A CN A2008100888968 A CNA2008100888968 A CN A2008100888968A CN 200810088896 A CN200810088896 A CN 200810088896A CN 101248890 A CN101248890 A CN 101248890A
- Authority
- CN
- China
- Prior art keywords
- sea cucumber
- weight portion
- high activity
- beche
- type high
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a nutrient-enhanced sea cucumber product with high activity, and a preparation method thereof, mainly solves the problems in the prior sea cucumber hydrolysate preparation method, including overhigh enzymolysis temperature, incomplete hydrolyzation, incomplete nutritional ingredients retention, and low content of active ingredients such as low molecular polypeptides, amino acids, active mucopolysaccharides and sea cucumber saponins. The technical proposal comprises the steps of subjecting sea cucumber to enzymolysis at a low temperature, and shearing large molecules in sea cucumber that are hardly absorbed by human body into small molecules that are easily absorbed. The processes from shopping sea cucumber to liquid sea cucumber processing can be finished within 5 hours, and completely retain the nutritional ingredients of sea cucumber. The finished product has strong biological activity; is rich in effective ingredients that can be easily utilized by human body, such as collagen protein, hydrolyzed polypeptides, amino acids, acidic polysaccharides and sea cucumber glycosides; and has the effects of resisting tumor, resisting bacteria, enhancing immunity and hemopoietic function, and relieving fatigue.
Description
Technical field
The present invention relates to a kind of marine product and preparation method thereof, especially a kind of nutrition reinforced type high activity beche-de-mer products and preparation method thereof.
Background technology
Traditional sea cucumber eating method is that water is sent out or high pressure, all need this process of high temperature process, processed complex and process technology that need be certain, the acid mucopolysaccharide in the sea cucumber, sea cucumber polypeptide, sea cucumber saponin, amino acid isoreactivity material and nutritional labeling are suffered to destroy significantly after heating through high temperature.
China Patent No. is CN1286926A disclosed " a kind of sea cucumber hydrolysate series of products and a processing method thereof ", its preparation method is with fresh sea cucumber or sends out the dried sea-cucumber that makes, through washing, fragmentation, enzyme hydrolysis, the enzyme that goes out, filter, take off raw meat processing and handle, enzymolysis process adopts double-enzyme hydrolysis or single enzymolysis, degree of hydrolysis is 50-75%, and active component and weight percent content that the enzymolysis liquid product contains are respectively: the bioactive peptide 5-20% of 2-10 peptide, amino acid/11 0-55%, holothurin 0.1-1.5%, mucopolysaccharide 0.5-5%.China Patent No. is CN1633910 disclosed " production method of holothurian oral liquid ", its technology is with the sea cucumber defibrination, and in sea cucumber milk, be incorporated as two kinds of neutral proteinases of the 1-3 ‰ of its weight, its weight proportion is 1: 1, be under 55-65 ℃ the condition hydrolysis 4-6 hour in temperature, be to keep 10-15 minute under 75-85 ℃ the condition in temperature, the sea cucumber hydrolyzate, homogeneous, filtration.China Patent No. is that to disclose a kind of be method and the product that raw material carries out enzymolysis with the sea cucumber to CN1561844A disclosed " method of enzyme hydrolysis sea cucumber and products thereof ", its preparation method is: fresh sea cucumber or dried sea-cucumber are cleaned, smash the sea cucumber aqueous solution of producing different sea cucumber concentration, and the compound protease that adds sea cucumber weight 0.15-0.45% in the sea cucumber aqueous solution carries out enzymolysis.
Above document discloses product and the preparation technology thereof that fresh sea cucumber or dried sea-cucumber obtain behind enzymolysis, its shortcoming is that preliminary treatment sea cucumber and enzymolysis process carry out under higher temperature in normal temperature environment, make the enzymolysis liquid hydrolysis that makes incomplete, the sea cucumber nutrient composition that is not kept perfectly, micromolecule polypeptide in the product that makes, amino acid, active mucopolysaccharide and sea cucumber saponin isoreactivity content of material are few, thereby influence the effect of absorption by human body and product.
Summary of the invention
The objective of the invention is,, enzymolysis liquid hydrolysis too high at hydrolysis temperature among the existing sea cucumber hydrolyzate preparation technology not exclusively, nutritional labeling keeps in imperfect, the product defectives such as micromolecule polypeptide, amino acid, active mucopolysaccharide and sea cucumber saponin isoreactivity content of material are few, and the applicant has invented a kind of nutrition reinforced type high activity beche-de-mer products and preparation method thereof.
A kind of nutrition reinforced type high activity beche-de-mer products provided by the present invention and preparation method thereof, it is characterized in that selecting for use the wild fresh and alive stichopus japonicus in Weihai, under the low-temperature condition with the sea cucumber enzymolysis, and the big molecule that is difficult to be absorbed by the body in the sea cucumber cut into the little molecule that very easily is absorbed by the body, be processed into the liquid sea cucumber from choosing of sea cucumber and in 5 hours, finish, the complete nutritional labeling that keeps sea cucumber.The beche-de-mer products that makes is rich in collagen, hydrolyzed peptide, amino acid, acidic polysaccharose and the sea cucumber saponin isoreactivity material that human body very easily utilizes, and has effect such as improve the health, delay senility.Compared with prior art, the liquid sea cucumber of the present invention's preparation is not added any anticorrisive agent, by adding xylitol, lotus seeds, hawthorn, potassium alginate, make the nutritional labeling of liquid sea cucumber abundanter, taste is purer, and is active high, helps absorption by human body more.
Nutrition reinforced type high activity beche-de-mer products provided by the present invention can also make beche-de-mer products taste of the present invention and nutrition variation more by the stoste of adding Momordica grosvenori, matrimony vine, genseng extraction.
Product of the present invention contains acidic polysaccharose, sea cucumber polypeptide, sea cucumber saponin isoreactivity material, human organ there is stronger repair function, macrophage, neutrophil leucocyte in the energy human activin blood cell, inducing cell activation, accelerate cell differentiation increment, improve the phagocytic function of macrophage.In addition, product also contains trace elements iron, zinc, selenium, vanadium and vitamin B2 and the B6 that exceeds several times than traditional beche-de-mer.
Nutrition reinforced type high activity beche-de-mer products provided by the invention has stronger physiologically active, comprising: antitumor action, antibacterial action, raising immunity and hematopoiesis function effect and antifatigue effect etc.:
(1) antitumor: product of the present invention contains the sea cucumber after a large amount of hydrolysis, anti-tumor factor wherein forms the inhibitory action that highly significant is all arranged with growth to the colony of tumour cell, observe PBATF (ocean bidentata anti-tumor factor) to Mice Bearing Lewis Lung Cancer, mouse H22 liver cancer, human body lung cancer tumour LAX, the depression effect of human hepatocellular tumour OGY and the growth of rat Walker-256 carcinosarcoma, the result shows the curative effect of PBATF to Mice Bearing Lewis Lung Cancer armpit subcutaneous vaccination model, high dose group 5mg/kg ip * 8qd scheme has 56.89% tumour inhibiting rate, the tumour inhibiting rate of middle dosage group 1mg/kg ip * 8qd scheme is 52.00%, and the tumour inhibiting rate of low dose group 0.5mg/kgip * 8qd scheme is 33.61%; Curative effect to rat Walker-carcinosarcoma armpit subcutaneous vaccination model, high dose group 5mg/kgip * 8qd scheme has 64.15% tumour inhibiting rate, the tumour inhibiting rate of middle dosage group 1mg/kg ip * 8qd scheme is 55.82%, and the tumour inhibiting rate of low dosage 0.5mg/kgip * 8qd scheme is 44.05%; Curative effect to human body adenocarcinoma of lung LAX armpit subcutaneous vaccination model, high dose group 5mg/kgip * 8qd scheme has 56.02% tumour inhibiting rate, the tumour inhibiting rate of middle dosage group 1mg/kg ip * 8qd scheme is 50.60%, and the tumour inhibiting rate of low dose group 0.5mg/kgip * 8qd scheme is 40.36%; Curative effect to human hepatocellular QGY armpit subcutaneous vaccination model, high dose group 5mg/kgip * 8qd scheme has 52.07% tumour inhibiting rate, the tumour inhibiting rate of middle dosage group 1mg/kg ip * 8qd scheme is 48.31%, and the tumour inhibiting rate of low dose group 0.5mg/kgip * 8qd scheme is 33.14%; Curative effect to rat liver cancer H22 armpit subcutaneous vaccination model, high dose group 5mg/kgip * 10qd scheme has 59.25% tumour inhibiting rate, the tumour inhibiting rate of middle dosage group 1mg/kgip * 10qd scheme is 57.74%, and the tumour inhibiting rate of low dose group 0.5mg/kg ip * 10qd scheme is 45.28%.
(2) antibacterial action: antibacterial result shows, water-soluble selenka contained in the product of the present invention all has significant bacteriostatic activity to fission yeast, saccharomycete brewer's yeast, Candida albicans, grape anthracnose, the former bacterium of cucumber fusarium axysporum and mould aspergillus niger, and its minimum inhibitory concentration MIC value is respectively 0.002,0.016,0.063,0.063,0.125 and 0.250mg/mL.
(3) immunological regulation and hematopoiesis function: the sea cucumber in the product of the present invention have the immunocompetence of immunoregulation effect and collaborative vaccine, stimulates the growth of marrow red blood cell simultaneously;
(4) antifatigue effect.
The method of nutrition reinforced type high activity beche-de-mer products of the present invention, be about to the method for sea cucumber hydrolyzate, lotus seeds leaching liquor, hawthorn leaching liquor, potassium alginate and xylitol mixing homogeneous, it mainly is the complete nutritional labeling that keeps sea cucumber, can not make the nutritional labeling of sea cucumber destroyed because of high temperature, and make it become the easier nutrient solution that is absorbed by the body.
The present invention realizes by following technological means:
A kind of nutrition reinforced type high activity beche-de-mer products comprises the product that can be processed into by the following raw materials according component, and the consumption of this raw material components is as follows:
Live body sea cucumber: 60-74 weight portion;
Xylitol: 10-27 weight portion;
Lotus seeds: 1-8 weight portion;
Hawthorn: 1-4 weight portion;
Potassium alginate: 1-4 weight portion.
Nutrition reinforced type high activity beche-de-mer products of the present invention is characterized in that the consumption of described raw material components can also comprise: Momordica grosvenori 3-6 weight portion, genseng 5-7 weight portion, matrimony vine 2-4 weight portion.
A kind of preparation method of nutrition reinforced type high activity beche-de-mer products is characterized in that this method may further comprise the steps:
(1) used raw material components consumption is a live body sea cucumber 60-74 weight portion, xylitol 10-27 weight portion, lotus seeds 1-8 weight portion, hawthorn 1-4 weight portion, potassium alginate 1-4 weight portion.
(2) select the live body sea cucumber for use, in 0-10 ℃ seawater, remove sea cucumber intestine, and clean the sea cucumber body go behind the intestines, make it clean;
(3) above-mentioned clean sea cucumber is broken into sea cucumber milk with stainless steel pulverizing machine, after the colloid mill fine grinding, put into thermostatic tank, under 0-10 ℃, using organic acid adjusting pH value is 3.5-6.5, and is incorporated as the protease of the 1-6 ‰ of sea cucumber milk weight in thermostatic tank;
(4) sea cucumber milk is heated up, under 40-70 ℃, kept 2-12 hour, make the sea cucumber milk complete hydrolysis, the liquid sea cucumber of complete hydrolysis is warming up to 90-100 ℃ rapidly, kept 3-20 minute, the abundant deactivation of enzyme, must high activity liquid sea cucumber mother liquor after the filtration;
(5) lotus seeds are put into the extracting at constant temperature jar, be incorporated as the water that the 12-15 of lotus seeds gross weight doubly measures, soak at room temperature 6-8 hour, again 70-90 ℃ of following lixiviate after 1 hour, filter to get filtrate, again filter residue is incorporated as the water that the 8-15 of lotus seeds gross weight doubly measures, under 70-90 ℃ of lixiviate 30 minutes, filter to get filtrate, twice filtrate merging is concentrated into 3-8 times of lotus seeds gross weight;
(6) hawthorn is put into the extracting at constant temperature jar, add as the water that the 6-7 of hawthorn gross weight doubly measures, soak at room temperature 8-10 hour, again 70-90 ℃ of following lixiviate after 1 hour, filter to get filtrate, again filter residue is incorporated as the water that the 3-4 of hawthorn gross weight doubly measures, under 70-90 ℃ of lixiviate 30 minutes, filter to get filtrate, twice filtrate merging is concentrated into 2-4 times of hawthorn gross weight;
(7) potassium alginate and xylitol are mixed after, add deionized water, again behind the colloid mill homogeneous liquid;
(8) will mix by the extract that step (4), (5), (6), (7) obtain, carry out homogeneous, be heated to 80-100 ℃ and lasting after 10 minutes, filter with homogenizer.
Live body sea cucumber of the present invention is to select wild fresh and alive " stichopus japonicus " of growth more than 3 years or 3 years for use, is good with the stichopus japonicus of 3-5.
Organic acid of the present invention can be a kind of in tartaric acid, malic acid, oxalic acid or the ascorbic acid.
A kind of in the protease of the present invention complex enzyme that can be bromelain, papain or bromelain and papain mix in 3: 1 ratios.
Lotus seeds of the present invention need be crushed to the 80-100 order in advance.
After product of the present invention spray-dried (baking temperature is 40-60 ℃) or the vacuum drying (vacuum is more than the 0.06Mpa), must strengthen the sea cucumber powder.
Raw material components of the present invention also comprises Momordica grosvenori, genseng, matrimony vine; With Momordica grosvenori 3-6 weight portion, genseng 5-7 weight portion, matrimony vine 2-4 weight portion is smashed to pieces, the 5-8 times of water gaging that adds Momordica grosvenori, genseng, matrimony vine gross weight, under 90-100 ℃, boiling 1-3 hour, filter Chinese medicine extract, Chinese medicine extract is mixed with the filtrate that step (8) obtains, pass through heating, homogeneous, sterilization again, get the sea cucumber nutrient oral liquid.
Weight portion of the present invention can be gram, kilogram (kilogram), liang, jin, ton etc.
The specific embodiment
Below for the key technical indexes of product of the present invention:
(1) strengthens the sea cucumber powder
Protein 〉=15%; Mixtures of polypeptides 〉=6%; Amino acid 〉=0.2%; Total number of bacteria (individual/g)≤30000; Coliform (individual/100g)≤30; Pathogenic bacteria do not detect.
(2) sea cucumber nutrient oral liquid
Polysaccharide (mg/g) 〉=100; Polypeptide (mg/L) 〉=500; Total number of bacteria (individual/ml)≤30000; Coliform (individual/100ml)≤30; Pathogenic bacteria do not detect.
Below in conjunction with embodiment preparation method of the present invention is described in further detail.
Embodiment 1
A kind of preparation method of nutrition reinforced type high activity beche-de-mer products, used raw material components consumption is a live body sea cucumber 60-74 weight portion, xylitol 10-27 weight portion, lotus seeds 1-8 weight portion, hawthorn 1-4 weight portion, potassium alginate 1-4 weight portion may further comprise the steps:
(1) selects 55 kilograms of the wild stichopus japonicus of live body for use, in 0 ℃ seawater, remove sea cucumber intestine, and clean the sea cucumber body go behind the intestines, make it clean;
(2) above-mentioned clean sea cucumber is broken into sea cucumber milk with stainless steel pulverizing machine, after the colloid mill fine grinding, put into 0 ℃ of thermostatic tank, using tartaric acid adjusting pH value is 6.5, adds papain (800,000 u/g) 1 ‰, stirs;
(3) sea cucumber milk is warming up to 40 ℃, kept 12 hours, make the sea cucumber milk complete hydrolysis, the liquid sea cucumber of complete hydrolysis is warming up to 90 ℃ rapidly, kept 20 minutes,, filter and obtain high activity liquid sea cucumber mother liquor the abundant deactivation of enzyme;
(4) lotus seeds (being crushed to 80 orders in advance) are put into the extracting at constant temperature jar for 1 kilogram, add 12 kilograms in water, soak at room temperature 6 hours,, filter to get filtrate after 1 hour 70 ℃ of following lixiviates again, again filter residue is added 10 kilograms in water, 70 ℃ of lixiviates 30 minutes filter to get filtrate, and twice filtrate merging is concentrated into 4 kilograms;
(5) hawthorn is put into the extracting at constant temperature jar for 2 kilograms, add 12 kilograms in water, soak at room temperature 10 hours,, filter to get filtrate after 1 hour 90 ℃ of following lixiviates again, again filter residue is added 6 kilograms in water, 80 ℃ of lixiviates 30 minutes filter to get filtrate, and twice filtrate merging is concentrated into 4 kilograms;
(6),, get liquid through the colloid mill homogeneous again with 20 kilograms of mobile aseptic deionized water dissolvings with 1 kilogram of potassium alginate (powdery, market sale product) and 20 kilograms of mixing of xylitol (powdery, market sale product);
(7) will be mixed by the extract that step (3), (4), (5), (6) obtain, water through the homogenizer homogeneous, is heated to 100 ℃ and lasting 10 minutes quantitatively to 82 kilograms, and filtration, tinning, microwave sterilization get nutrition reinforced type high activity liquid sea cucumber.
Embodiment 2
A kind of preparation method of nutrition reinforced type high activity beche-de-mer products, used raw material components consumption is a live body sea cucumber 60-74 weight portion, xylitol 10-27 weight portion, lotus seeds 1-8 weight portion, hawthorn 1-4 weight portion, potassium alginate 1-4 weight portion may further comprise the steps:
(1) selects 50 kilograms of the wild stichopus japonicus of live body for use, in 5 ℃ seawater, remove sea cucumber intestine, and clean the sea cucumber body go behind the intestines, make it clean;
(2) above-mentioned clean sea cucumber is broken into sea cucumber milk with stainless steel pulverizing machine, after the colloid mill fine grinding, put into 5 ℃ of thermostatic tanks, using malic acid adjusting pH value is 3.5, adds bromelain (2,000,000 u/g) 2 ‰, stirs;
(3) sea cucumber milk is warming up to 70 ℃, kept 2 hours, make the sea cucumber milk complete hydrolysis, the liquid sea cucumber of complete hydrolysis is warming up to 100 ℃ rapidly, kept 3 minutes, filter and obtain high activity liquid sea cucumber mother liquor;
(4) lotus seeds (being crushed to 100 orders in advance) are put into the extracting at constant temperature jar for 2 kilograms, add 30 kilograms in water, soak at room temperature 8 hours, again 80 ℃ of following lixiviates after 1 hour, filter liquid, again filter residue is added 30 kilograms in water, 80 ℃ of lixiviates 30 minutes filter to get filtrate, and twice filtrate merging is concentrated into 6 kilograms;
(5) hawthorn is put into the extracting at constant temperature jar for 1.5 kilograms, add 9 kilograms in water, soak at room temperature 9 hours,, filter to get filtrate after 1 hour 90 ℃ of following lixiviates again, again filter residue is added 6 kilograms in water, 70 ℃ of lixiviates 30 minutes filter to get filtrate, and twice filtrate merging is concentrated into 3 kilograms;
(6) 15 kilograms of 1 kilogram of potassium alginate (powdery, market sale product) and xylitols (powdery, market sale product) are mixed after, add 25 kilograms of mobile aseptic deionized waters, get liquid through the colloid mill homogeneous again;
(7) will be mixed by the extract that step (3), (4), (5), (6) obtain, through the homogenizer homogeneous, be heated to 80 ℃ and lasting 10 minutes, filtration, tinning, microwave sterilization get nutrition reinforced type high activity liquid sea cucumber.
Embodiment 3
A kind of preparation method of nutrition reinforced type high activity beche-de-mer products, used raw material components consumption is a live body sea cucumber 60-74 weight portion, xylitol 10-27 weight portion, lotus seeds 1-8 weight portion, hawthorn 1-4 weight portion, potassium alginate 1-4 weight portion may further comprise the steps:
(1) selects 60 kilograms of the wild stichopus japonicus of live body, in 10 ℃ seawater, remove sea cucumber intestine, and clean the sea cucumber body go behind the intestines, make it clean;
(2) above-mentioned clean sea cucumber is broken into sea cucumber milk with stainless steel pulverizing machine, after the colloid mill fine grinding, put into 10 ℃ of thermostatic tanks, using ascorbic acid adjusting pH value is 5, adds bromelain (800,000 u/g) 6 ‰, stirs;
(3) sea cucumber milk is warming up to 50 ℃, kept 6 hours, make the sea cucumber milk complete hydrolysis, the liquid sea cucumber of complete hydrolysis is warming up to 95 ℃ rapidly, kept 10 minutes, filter and obtain high activity liquid sea cucumber mother liquor;
(4) lotus seeds (being crushed to 90 orders in advance) are put into the extracting at constant temperature jar for 3.5 kilograms, add 49 kilograms in water, soak at room temperature 8 hours, again 90 ℃ of following lixiviates after 1 hour, filter to get filtrate, again filter residue is added 35 kilograms in water, 90 ℃ of lixiviates 30 minutes, filter to get filtrate, twice filtrate merging is concentrated into 28 kilograms;
(5) hawthorn is put into the extracting at constant temperature jar for 1 kilogram, add 6 kilograms in water, soak at room temperature 10 hours,, filter to get filtrate after 1 hour 90 ℃ of following lixiviates again, again filter residue is added 3 kilograms in water, 90 ℃ of lixiviates 30 minutes filter to get filtrate, and twice filtrate merging is concentrated into 2 kilograms;
(6) 10 kilograms of 1 kilogram of potassium alginate (powdery, market sale product) and xylitols (powdery, market sale product) are mixed in proportion after, with 15 kilograms of mobile aseptic deionized waters dissolvings, get liquid through the colloid mill homogeneous again;
(7) will be mixed by the extract that step (3), (4), (5), (6) obtain, through the homogenizer homogeneous, be heated to 90 ℃ and lasting 10 minutes, filtration, tinning, microwave sterilization get nutrition reinforced type high activity liquid sea cucumber.
Embodiment 4
A kind of preparation method of nutrition reinforced type high activity beche-de-mer products, used raw material components consumption is a live body sea cucumber 60-74 weight portion, xylitol 10-27 weight portion, lotus seeds 1-8 weight portion, hawthorn 1-4 weight portion, potassium alginate 1-4 weight portion may further comprise the steps:
(1) selects 70 kilograms of the wild stichopus japonicus of live body, in 7 ℃ seawater, remove sea cucumber intestine, and clean the sea cucumber body go behind the intestines, make it clean;
(2) above-mentioned clean sea cucumber is broken into sea cucumber milk with stainless steel pulverizing machine, after the colloid mill fine grinding, put into 6 ℃ of thermostatic tanks, using oxalic acid adjusting pH value is 6.5, add bromelain (2,000,000 u/g) and papain (800,000 u/g) complex enzyme 5 ‰, stir in ratio mixing in 3: 1;
(3) sea cucumber milk is warming up to 60 ℃, kept 8 hours, make the sea cucumber milk complete hydrolysis, the liquid sea cucumber of complete hydrolysis is warming up to 95 ℃ rapidly, kept 15 minutes, filter and obtain 30 kilograms in high activity liquid sea cucumber mother liquor;
(4) lotus seeds (being crushed to 90 orders in advance) are put into the extracting at constant temperature jar for 5 kilograms, add 60 kilograms in water, soak at room temperature 7 hours,, filter to get filtrate after 1 hour 85 ℃ of following lixiviates again, again filter residue is added 40 kilograms in water, 80 ℃ of lixiviates 30 minutes filter to get filtrate, and twice filtrate merging is concentrated into 25 kilograms;
(5) hawthorn is put into the extracting at constant temperature jar for 1 kilogram, add 6.5 kilograms in water, soak at room temperature 9 hours,, filter to get filtrate after 1 hour 85 ℃ of following lixiviates again, again filter residue is added 3.5 kilograms in water, 85 times lixiviates 30 minutes, filter to get filtrate, twice filtrate merging is concentrated into 2.1 kilograms;
(6) 5 kilograms of 1 kilogram of potassium alginate (powdery, market sale product) and xylitols (powdery, market sale product) are mixed in proportion after, with 10 kilograms of mobile aseptic deionized waters dissolvings, get liquid through the colloid mill homogeneous again;
(7) will be mixed by the extract that step (3), (4), (5), (6) obtain, through the homogenizer homogeneous, be heated to 100 ℃ and lasting 10 minutes, filtration, canned, microwave sterilization get nutrition reinforced type high activity liquid sea cucumber.
Embodiment 5
With nutrition reinforced type high activity liquid sea cucumber under 40 ℃, spray-drying, or to place vacuum be the vavuum pump of 0.08Mpa, vacuum drying must be strengthened the sea cucumber powder.
Embodiment 6
With nutrition reinforced type high activity liquid sea cucumber under 60 ℃, spray-drying, in the vavuum pump of 0.07Mpa, vacuum drying must be strengthened the sea cucumber powder.
Embodiment 7
With nutrition reinforced type high activity liquid sea cucumber under 50 ℃, spray-drying, in the vavuum pump of 0.10Mpa, vacuum drying must be strengthened the sea cucumber powder.
Embodiment 8
With 3 kilograms of Momordica grosvenoris, 6 kilograms of gensengs, matrimony vine is smashed to pieces for 3 kilograms, adds 60 kg water under 90 ℃, and boiling was filtered after 1 hour, made Chinese medicine extract; Nutrition reinforced type high activity liquid sea cucumber and Chinese medicine extract in 1: 1 ratio mixing, are passed through heating, homogeneous, sterilization again, the nutrition reinforced type high activity beche-de-mer oral liquid.
Embodiment 9
With 4 kilograms of Momordica grosvenoris, 5 kilograms of gensengs, matrimony vine is smashed to pieces for 2 kilograms, adds 88 kg water under 90 ℃, and boiling was filtered after 2 hours, made Chinese medicine extract; Nutrition reinforced type high activity liquid sea cucumber and Chinese medicine extract in 2: 1 ratio mixings, are passed through heating, homogeneous, sterilization again, the nutrition reinforced type high activity beche-de-mer oral liquid.
Embodiment 10
With 6 kilograms of Momordica grosvenoris, 7 kilograms of gensengs, matrimony vine is smashed to pieces for 4 kilograms, adds 102 kg water under 90 ℃, and boiling was filtered after 3 hours, made Chinese medicine extract; Nutrition reinforced type high activity liquid sea cucumber and Chinese medicine extract in 3: 1 ratio mixings, are passed through heating, homogeneous, sterilization again, the nutrition reinforced type high activity beche-de-mer oral liquid.
Claims (10)
1, a kind of nutrition reinforced type high activity beche-de-mer products comprises the product that can be processed into by the following raw materials according component, and the consumption of this raw material components is as follows:
Live body sea cucumber: 60-74 weight portion;
Xylitol: 10-27 weight portion;
Lotus seeds: 1-8 weight portion;
Hawthorn: 1-4 weight portion;
Potassium alginate: 1-4 weight portion.
2, nutrition reinforced type high activity beche-de-mer products according to claim 1 is characterized in that the consumption of described raw material components comprises: Momordica grosvenori 3-6 weight portion, genseng 5-7 weight portion, matrimony vine 2-4 weight portion.
3, a kind of method for preparing nutrition reinforced type high activity beche-de-mer products as claimed in claim 1 is characterized in that this method may further comprise the steps:
(1) used raw material components consumption is a live body sea cucumber 60-74 weight portion, xylitol 10-27 weight portion, lotus seeds 1-8 weight portion, hawthorn 1-4 weight portion, potassium alginate 1-4 weight portion.
(2) select the live body sea cucumber for use, in 0-10 ℃ seawater, remove sea cucumber intestine, and clean the sea cucumber body go behind the intestines, make it clean;
(3) above-mentioned clean sea cucumber is broken into sea cucumber milk with stainless steel pulverizing machine, after the colloid mill fine grinding, put into thermostatic tank, under 0-10 ℃, using organic acid adjusting pH value is 3.5-6.5, and is incorporated as the protease of the 1-6 ‰ of sea cucumber milk weight in thermostatic tank;
(4) sea cucumber milk is heated up, under 40-70 ℃, kept 2-12 hour, make the sea cucumber milk complete hydrolysis, the liquid sea cucumber of complete hydrolysis is warming up to 90-100 ℃ rapidly, kept 3-20 minute, the abundant deactivation of enzyme, must high activity liquid sea cucumber mother liquor after the filtration;
(5) lotus seeds are put into the extracting at constant temperature jar, be incorporated as the water that the 12-15 of lotus seeds gross weight doubly measures, soak at room temperature 6-8 hour, again 70-90 ℃ of following lixiviate after 1 hour, filter to get filtrate, filter residue is incorporated as the water that the 8-15 of lotus seeds gross weight doubly measures, 70-90 ℃ of following lixiviate 30 minutes, filter to get filtrate, twice filtrate merging is concentrated into 3-8 times of lotus seeds gross weight;
(6) hawthorn is put into the extracting at constant temperature jar, add as the water that the 6-7 of hawthorn gross weight doubly measures, soak at room temperature 8-10 hour, again 70-90 ℃ of following lixiviate after 1 hour, filter to get filtrate, filter residue is incorporated as the water that the 3-4 of hawthorn gross weight doubly measures, 70-90 ℃ of following lixiviate 30 minutes, filter to get filtrate, twice filtrate merging is concentrated into 2-4 times of hawthorn gross weight;
(7) potassium alginate and xylitol are mixed after, add deionized water, again behind the colloid mill homogeneous liquid;
(8) will mix by the extract that step (4), (5), (6), (7) obtain, carry out homogeneous, be heated to 80-100 ℃ and lasting after 10 minutes, filter with homogenizer.
4, live body sea cucumber according to claim 3 is to select wild fresh and alive " stichopus japonicus " of growth more than 3 years or 3 years for use.
5, the preparation method of nutrition reinforced type beche-de-mer products according to claim 3 is characterized in that: described organic acid is a kind of in tartaric acid, malic acid, acetate, citric acid or the ascorbic acid.
6, the preparation method of nutrition reinforced type high activity beche-de-mer products according to claim 3 is characterized in that: a kind of in the described protease complex enzyme that can be bromelain, papain or bromelain and papain mix in 3: 1 ratios.
7, the preparation method of nutrition reinforced type high activity beche-de-mer products according to claim 3 is characterized in that: described lotus seeds need be crushed to the 80-100 order in advance.
8, the preparation method of nutrition reinforced type high activity beche-de-mer products according to claim 3 is characterized in that: with the filtrate that step (8) obtains, through spray-drying or vacuum drying, must strengthen the sea cucumber powder.
9, the preparation method of nutrition reinforced type high activity beche-de-mer products according to claim 8 is characterized in that: described spray-drying or vacuum drying, its baking temperature are 40-60 ℃, and vacuum is 0.06Mpa-0.1Mpa.
10, a kind of method for preparing nutrition reinforced type high activity beche-de-mer products according to claim 3 is characterized in that described raw material components also comprises Momordica grosvenori, genseng, matrimony vine; With Momordica grosvenori 3-6 weight portion, genseng 5-7 weight portion, matrimony vine 2-4 weight portion is smashed to pieces, the 5-8 times of water gaging that adds Momordica grosvenori, genseng, matrimony vine gross weight, under 90-100 ℃, boiling 1-3 hour, filter Chinese medicine extract, Chinese medicine extract is mixed with the filtrate after the step (8), pass through heating, homogeneous, sterilization again, get the nutrition reinforced type high activity beche-de-mer oral liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100888968A CN101248890A (en) | 2008-04-03 | 2008-04-03 | Nutrition reinforced type high activity beche-de-mer products and method of preparing the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100888968A CN101248890A (en) | 2008-04-03 | 2008-04-03 | Nutrition reinforced type high activity beche-de-mer products and method of preparing the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101248890A true CN101248890A (en) | 2008-08-27 |
Family
ID=39952634
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100888968A Pending CN101248890A (en) | 2008-04-03 | 2008-04-03 | Nutrition reinforced type high activity beche-de-mer products and method of preparing the same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101248890A (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974394A (en) * | 2010-10-20 | 2011-02-16 | 大连海晏堂生物有限公司 | Nano sea cucumber wine and preparation method thereof |
| CN102743410A (en) * | 2012-06-27 | 2012-10-24 | 宁波大学 | Use of Acaudina leucoprocta polysaccharide in pharmacy |
| CN103284268A (en) * | 2013-06-17 | 2013-09-11 | 钦州市钦州港永健水产贸易有限公司 | Preparation method of trepang solid beverage |
| CN103371388A (en) * | 2012-04-19 | 2013-10-30 | 日加满饮品(上海)有限公司 | Fruity beverage and preparation method thereof |
| CN103652898A (en) * | 2013-12-11 | 2014-03-26 | 威海力元海洋生物科技有限公司 | Solid composition containing holothurian and chlorella pyrenoidosa and preparation method thereof |
| CN103815411A (en) * | 2014-03-19 | 2014-05-28 | 张伟 | Health care jelly-like canned sea cucumbers and preparation method thereof |
| CN103897407A (en) * | 2012-12-27 | 2014-07-02 | 辽宁阿里郎生物工程股份有限公司 | Anti-doze sea cucumber polypeptide edible film and preparation method thereof |
| CN104473156A (en) * | 2014-11-25 | 2015-04-01 | 荣成宏业海洋科技有限公司 | Processing method of sea cucumber oral liquid |
| CN104544294A (en) * | 2015-01-15 | 2015-04-29 | 青岛老尹家海参股份有限公司 | Processing method for high-nutrition sea cucumber |
| CN104705645A (en) * | 2015-03-25 | 2015-06-17 | 渤海大学 | Sea cucumber peptide oral solution and formula thereof |
| CN104886653A (en) * | 2015-06-10 | 2015-09-09 | 威海紫光科技园有限公司 | Holothurian and American ginseng health-care product and preparation method thereof |
| CN105063151A (en) * | 2015-06-10 | 2015-11-18 | 威海益合元生物科技有限公司 | Sea urchin peptide oral liquid and preparation method thereof |
| CN105639392A (en) * | 2016-01-06 | 2016-06-08 | 威海特伦斯生物工程有限公司 | Production method of selenium-rich sea cucumber solid beverage |
| CN106071539A (en) * | 2016-06-20 | 2016-11-09 | 宁波高新区科博汇科技有限公司 | A kind of beche-de-mer in East China Sea nutrient health-care beverage and preparation method thereof |
| CN106434194A (en) * | 2016-08-17 | 2017-02-22 | 任浩楠 | Anticoagulation and anti-tumor polypeptide health-care wine and preparing method thereof |
| CN106726812A (en) * | 2015-11-20 | 2017-05-31 | 威海市养元多鲜食海参有限公司 | A kind of sea cucumber fruit-vegetable facial mask |
| CN110419731A (en) * | 2019-07-24 | 2019-11-08 | 大连真擎肽海洋科技有限公司 | A kind of seafood health care product |
| CN112794927A (en) * | 2021-03-11 | 2021-05-14 | 烟台和易万家生物科技有限公司 | Method for extracting sea cucumber polysaccharide with high purity and high extraction rate |
-
2008
- 2008-04-03 CN CNA2008100888968A patent/CN101248890A/en active Pending
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974394B (en) * | 2010-10-20 | 2012-07-04 | 大连海晏堂生物有限公司 | Nano sea cucumber wine and preparation method thereof |
| CN101974394A (en) * | 2010-10-20 | 2011-02-16 | 大连海晏堂生物有限公司 | Nano sea cucumber wine and preparation method thereof |
| CN103371388B (en) * | 2012-04-19 | 2015-05-06 | 日加满饮品(上海)有限公司 | Fruity beverage preparation method |
| CN103371388A (en) * | 2012-04-19 | 2013-10-30 | 日加满饮品(上海)有限公司 | Fruity beverage and preparation method thereof |
| CN102743410A (en) * | 2012-06-27 | 2012-10-24 | 宁波大学 | Use of Acaudina leucoprocta polysaccharide in pharmacy |
| CN103897407B (en) * | 2012-12-27 | 2016-01-20 | 辽宁阿里郎生物工程股份有限公司 | A kind of anti-drowsy sea cucumber polypeptide edible film and preparation method thereof |
| CN103897407A (en) * | 2012-12-27 | 2014-07-02 | 辽宁阿里郎生物工程股份有限公司 | Anti-doze sea cucumber polypeptide edible film and preparation method thereof |
| CN103284268A (en) * | 2013-06-17 | 2013-09-11 | 钦州市钦州港永健水产贸易有限公司 | Preparation method of trepang solid beverage |
| CN103652898A (en) * | 2013-12-11 | 2014-03-26 | 威海力元海洋生物科技有限公司 | Solid composition containing holothurian and chlorella pyrenoidosa and preparation method thereof |
| CN103815411A (en) * | 2014-03-19 | 2014-05-28 | 张伟 | Health care jelly-like canned sea cucumbers and preparation method thereof |
| CN103815411B (en) * | 2014-03-19 | 2015-08-19 | 张伟 | One freezes shape sea cucumber health care can and preparation method thereof |
| CN104473156A (en) * | 2014-11-25 | 2015-04-01 | 荣成宏业海洋科技有限公司 | Processing method of sea cucumber oral liquid |
| CN104544294A (en) * | 2015-01-15 | 2015-04-29 | 青岛老尹家海参股份有限公司 | Processing method for high-nutrition sea cucumber |
| CN104705645A (en) * | 2015-03-25 | 2015-06-17 | 渤海大学 | Sea cucumber peptide oral solution and formula thereof |
| CN105063151A (en) * | 2015-06-10 | 2015-11-18 | 威海益合元生物科技有限公司 | Sea urchin peptide oral liquid and preparation method thereof |
| CN104886653A (en) * | 2015-06-10 | 2015-09-09 | 威海紫光科技园有限公司 | Holothurian and American ginseng health-care product and preparation method thereof |
| CN106726812A (en) * | 2015-11-20 | 2017-05-31 | 威海市养元多鲜食海参有限公司 | A kind of sea cucumber fruit-vegetable facial mask |
| CN105639392A (en) * | 2016-01-06 | 2016-06-08 | 威海特伦斯生物工程有限公司 | Production method of selenium-rich sea cucumber solid beverage |
| CN106071539A (en) * | 2016-06-20 | 2016-11-09 | 宁波高新区科博汇科技有限公司 | A kind of beche-de-mer in East China Sea nutrient health-care beverage and preparation method thereof |
| CN106434194A (en) * | 2016-08-17 | 2017-02-22 | 任浩楠 | Anticoagulation and anti-tumor polypeptide health-care wine and preparing method thereof |
| CN110419731A (en) * | 2019-07-24 | 2019-11-08 | 大连真擎肽海洋科技有限公司 | A kind of seafood health care product |
| CN112794927A (en) * | 2021-03-11 | 2021-05-14 | 烟台和易万家生物科技有限公司 | Method for extracting sea cucumber polysaccharide with high purity and high extraction rate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101248890A (en) | Nutrition reinforced type high activity beche-de-mer products and method of preparing the same | |
| CN102578363B (en) | Method for preparing small-molecule collagen polypeptide powder by using poultry skin and application of small-molecule collagen polypeptide powder in fruit juice beverage | |
| CN102228231A (en) | Ultramicro nutritional powder containing red dates or haws and gingers and manufacturing method thereof | |
| CN107125675A (en) | A kind of preparation method of blueberry residue flavouring-soy-sauce | |
| CN106858415A (en) | A kind of preparation method of the holothurian oral liquid of smelling removal | |
| CN104172411A (en) | Making method of black rice health care drink | |
| CN114480549A (en) | Bioactive peptide prepared from snail, and preparation method and application thereof | |
| CN107751697A (en) | Chlorella extract phycocyanin drink with function preparation method | |
| CN108771076B (en) | Preparation and redissolution method of composite curcumin myofibrillar protein solid beverage | |
| CN101524167A (en) | Small-molecule sea cucumber powder and small-molecule sea cucumber powder capsules | |
| JP4022747B2 (en) | Method for producing Agaricus koji extract | |
| CN110156908A (en) | A kind of extracting method of Blackfungus polyhexose | |
| CN113317374A (en) | Preparation method of fermented beverage | |
| CN103710244B (en) | A kind of preparation method of Truffle wine and the Truffle wine prepared by the method | |
| CN111587974B (en) | Beverage | |
| CN104686768A (en) | Sea cucumber compressed tablet candy capable of enhancing immunity with high efficiency | |
| CN101974394B (en) | Nano sea cucumber wine and preparation method thereof | |
| CN1276723C (en) | Water soluble royal jelly and its dry powder and method for preparing them | |
| CN114343143B (en) | Marine algae fermentation product and preparation method thereof | |
| CN103445151B (en) | Cordyceps militaris oral liquid and preparation method thereof | |
| CN105341238A (en) | Chlorella polypeptide tea and manufacturing method thereof | |
| CN108546620A (en) | A kind of deer ginseng small-molecular peptides nourish wine and preparation method thereof | |
| CN114128812A (en) | Ready-to-drink burdock bird's nest peptide liquid compound beverage easy to digest and preparation method thereof | |
| CN105918549A (en) | Ginger salt tea vinegar beverage and preparation method thereof | |
| CN113812466A (en) | Ginseng and walnut enzyme beverage and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20080827 |