CN105063151A - Sea urchin peptide oral liquid and preparation method thereof - Google Patents
Sea urchin peptide oral liquid and preparation method thereof Download PDFInfo
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- CN105063151A CN105063151A CN201510574046.9A CN201510574046A CN105063151A CN 105063151 A CN105063151 A CN 105063151A CN 201510574046 A CN201510574046 A CN 201510574046A CN 105063151 A CN105063151 A CN 105063151A
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- sea urchin
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- hemicentrotus seu
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses sea urchin peptide oral liquid and a preparation method thereof. The preparation method is characterized by comprising the following steps: after fresh sea urchins are stripped off, taking out sea urchin gonads, mixing the sea urchin gonads and water for grinding, adding lipase for enzymolysis degreasing, performing ultrafiltration to collect trapped fluid which is a sea urchin gonad coarse protein solution, performing periodical cooperative enzymolysis on the sea urchin gonad coarse protein solution by adopting bromelain and ginger protease, performing filtration and ultrafiltration on enzymatic hydrolysate, and mixing and concentrating to obtain the sea urchin peptide oral liquid. According to the method, the phenomenon of rancidity of the sea urchin gonad is effectively prevented; the utilization rate of sea urchin proteins is greatly increased, and the food safety is effectively guaranteed; furthermore, the taste of the sea urchin peptide oral liquid is improved, and due to the antioxidant activity, the stability of the sea urchin peptide oral liquid is greatly improved, and the expiration date is prolonged; furthermore, the sea urchin peptide oral liquid has auxiliary effects of adjusting immunity, protecting the liver, resisting tumors and aging on the sea urchin gonads.
Description
Technical field
The present invention relates to technical field of bioengineering, specifically a kind of sea urchin peptide oral liquid and preparation method thereof.
Background technology
The name of sea urchin begins to be loaded in " book on Chinese herbal medicine is original ", is that one is eating marine fishery widely all over the world.The sexual gland of sea urchin is gonad of Hemicentrotus seu Strongylocentrotus, containing a large amount of protein, amino acid, highly unsaturated fatty acids, carbohydrate and some physiologically active substances, not only has very high edibleness, also has fabulous medicinal function, has good prevention effect to cardiovascular diseases.Always there is the custom of eating sea urchin on the ground such as Chinese Liaoning, Shandong, Guangdong, Fujian and Hainan.Some countries of Japan, Malay Archipelago, South America and Mediterranean, also have the custom of eating sea urchin as France, Italy, Greece etc. are national, especially japanese people has a liking for eating sea urchin especially.As can be seen here, sea urchin is the marine resources that a kind of nutritive value is very high, has the wide market space, is of great significance its research tool.
Fresh-keeping and the storage problem of sea urchin is one of main restricting factor of sea urchin industry development.The easy self-dissolving of gonad of Hemicentrotus seu Strongylocentrotus, after sea urchin fishes for water outlet, places half to one, just may feel like jelly rotten, lose edibleness.Deep processing is carried out to sea urchin, not only can extend its quality guaranteed period, also can promote its resource value.At present, the sea urchin deep processed product on market mainly contains sea urchin roe paste, sea urchin gruel, alcohol sea urchin.In these products, the main existence form of sea urchin protein is macro-molecular protein, is unfavorable for absorbing of human body.As adopted proteolytic enzyme sea urchin protein to be carried out appropriateness hydrolysis, generating the sea urchin peptide that the little peptide that forms with 2--6 amino acid is main component, directly can be absorbed without digestion by human body.Compared with sea urchin protein, sea urchin peptide not only has higher nutritive value, but also have anti-oxidant, antitumor, improve the physiological function such as body immunity.The present invention adopts combined-enzyme method to carry out appropriateness hydrolysis to sea urchin to prepare sea urchin peptide oral liquid, not only more convenient edible, and sea urchin protein is hydrolyzed into small active peptides, improves its nutritive value and absorption rate.
Summary of the invention
The object of the invention is to solve above-mentioned the deficiencies in the prior art, provide one to be of high nutritive value, absorption rate is high, mouthfeel is pure and honest, sour-sweet moderate, sea urchin peptide oral liquid that delicate flavour is pure and preparation method thereof.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of sea urchin peptide oral liquid, is characterized in that by fresh gonad of Hemicentrotus seu Strongylocentrotus after degreasing, adopts combined-enzyme method to be prepared from.
The preparation method of sea urchin peptide oral liquid of the present invention take gonad of Hemicentrotus seu Strongylocentrotus as raw material, first lipase is adopted to be hydrolyzed, gonad of Hemicentrotus seu Strongylocentrotus crude protein solution is obtained after ultrafiltration, compound protease is adopted to carry out appropriateness hydrolysis again, make gonad of Hemicentrotus seu Strongylocentrotus proteolysis to below molecular weight 2000Da, again after filtration, the operation such as concentrated, allotment, obtain sea urchin peptide oral liquid.
The concrete steps of sea urchin peptide oral liquid of the present invention and preparation method thereof are as follows:
(1) new urchin is pushed aside, take out sexual gland, i.e. gonad of Hemicentrotus seu Strongylocentrotus;
(2) by gonad of Hemicentrotus seu Strongylocentrotus and deionized water mixing defibrination, add lipase and carry out enzymolysis, ultrafiltration, collect trapped fluid, be gonad of Hemicentrotus seu Strongylocentrotus crude protein solution;
(3) mixed with deionized water by gonad of Hemicentrotus seu Strongylocentrotus crude protein solution, stir evenly, heating, makes its appropriate sex change, adds prozyme and carry out enzymolysis after cooling;
(4), after enzymolysis terminates, the enzyme that carried out by enzymolysis solution going out, filtration, concentratedly sea urchin peptide solution is obtained;
(5) in sea urchin peptide solution, add fructus lycii extracted solution, hawthorn extract, nisin, allocate, obtain sea urchin peptide oral liquid.
Step of the present invention (1) concrete operations are: after being cleaned by the new urchin of select, pry open the soft shell of the radial prickle of black bands with scissors, dig out shape in shell, like the sea urchin egg of orange lobe yellow, remove internal organ with soup spoon.
The concrete operations of step of the present invention (2) are: 1:6 ~ 8 that added water by gonad of Hemicentrotus seu Strongylocentrotus mix defibrination, obtain gonad of Hemicentrotus seu Strongylocentrotus slurry 70 ~ 80 DEG C of heating 10 ~ 15min, then 40 ± 1 DEG C are cooled to, add lipase and carry out enzymolysis, after enzymolysis terminates, enzymolysis solution adopts and blocks molecular weight is that the ultra-filtration membrane of 10000Da carries out ultrafiltration, and cycles of concentration is 2 ~ 3 times, collect trapped fluid, obtain gonad of Hemicentrotus seu Strongylocentrotus crude protein solution.
The consumption of lipase of the present invention is 100 ~ 200u/g gonad of Hemicentrotus seu Strongylocentrotus, and enzymatic hydrolysis condition is pH8 ~ 10,40 ± 1 DEG C, time 2 ~ 4h.
Step of the present invention (3) concrete operations are mixed with the deionized water of 2 ~ 3 times of volumes by gonad of Hemicentrotus seu Strongylocentrotus crude protein solution, be heated to 90 ~ 100 DEG C and maintain 10 ~ 15min, to 45 ~ 50 DEG C after cooling, add food grade bromeline, after enzymolysis 2 ~ 3h, add 3 ~ 3.5g/L sucrose, abundant dissolving mixes, add food grade Ginger Protease, continue enzymolysis 3 ~ 4h
The consumption of bromeline of the present invention is 5800 ~ 6200u/g gonad of Hemicentrotus seu Strongylocentrotus, and Zingibain enzyme dosage is 50 ~ 100u/g gonad of Hemicentrotus seu Strongylocentrotus.
The concrete operations of step of the present invention (4) are: after the enzyme that gone out by enzymolysis solution 90 ~ 100 DEG C heating 10 ~ 15min, filtration or centrifugal method is adopted to carry out solid-liquid separation, remove a small amount of residue, obtain clear liquor, clear liquor adopts and blocks molecular weight is that the ultra-filtration membrane of 2000Da carries out ultrafiltration, and permeate carries out vacuum concentration in 70 ~ 80 DEG C, and vacuum tightness is 0.06 ~ 0.08MPa, cycles of concentration is 3 ~ 4 times, is sea urchin peptide solution.
Preparing fructus lycii extracted solution concrete operations in step of the present invention (5) is: take matrimony vine by the consumption of matrimony vine 60 ~ 65g/kg gonad of Hemicentrotus seu Strongylocentrotus, after deionized water rinsing, deionized water is added in 1:3 ~ 4, soak 2 ~ 3h, boiling 20 ~ 30min, two-layer gauze coarse filtration, collect filtrate and residue respectively, residue is pressed 1:2 ~ 3 and is added deionized water, boiling 15 ~ 20min, after two-layer filtered through gauze, merges the filtrate obtained for twice, adopt the Büchner funnel being covered with the thick diatomite filter of 4 ~ 6mm in advance to carry out suction filtration, clarification permeate is fructus lycii extracted solution.
Preparing hawthorn extract concrete operations in step of the present invention (5) is take hawthorn by the consumption of hawthorn 60 ~ 65g/kg gonad of Hemicentrotus seu Strongylocentrotus, stoning, after deionized water rinsing, deionized water is added in 1:3 ~ 4, soaks 2 ~ 3h, colloidal mill is pulled an oar, hawthorn slurry boiling 20 ~ 30min, two-layer gauze coarse filtration, collects filtrate, adopt the Büchner funnel being covered with the thick diatomite filter of 4 ~ 6mm in advance to carry out suction filtration, clarification permeate is hawthorn extract.
In step of the present invention (5), the consumption of nisin is 0.15 ~ 0.18g/kg sea cucumber peptide oral liquid.
Step of the present invention (5) concrete operations are: fructus lycii extracted solution, hawthorn extract and nisin are mixed with sea urchin peptide solution, are sea urchin peptide oral liquid.
The invention has the beneficial effects as follows:
1, the present invention uses prozyme to prepare sea urchin peptide, prozyme used is plant protease, particularly Ginger Protease, extract from ginger, ginger is the vegetables of dietotherapeutic, aboundresources, therefore uses Ginger Protease to produce sea urchin peptide oral liquid not only economical but also ensure that food safety;
2, the present invention adopts prozyme to be hydrolyzed the mode preparing sea urchin peptide stage by stage, first bromeline is adopted to carry out enzymolysis, NaCl in gonad of Hemicentrotus seu Strongylocentrotus has certain provide protection to its activity, then adopt Ginger Protease to continue enzymolysis, and use sucrose as its enzyme activator alive, collaborative bromelain hydrolyzate gonad of Hemicentrotus seu Strongylocentrotus albumen, not only increase degree of hydrolysis, increase polypeptide and free aminoacid content, and enhance the delicate flavour of sea urchin peptide oral liquid, be more conducive to human body digesting and assimilating food;
3, fructus lycii extracted solution and hawthorn extract and sea urchin peptide solution are allocated by the present invention, and do not add any chemical substance, make sea urchin peptide oral liquid mouthfeel pure and honest, sour-sweet moderate, delicate flavour is pure.Abundant lycium barbarum polysaccharide, organic acid, glycoside and Flavonoid substances is contained in matrimony vine and hawthorn extract, its anti-oxidant activity substantially increases the stability of sea urchin peptide oral liquid, extend the shelf life, at immunity moderation, protect the liver, antitumor, anti-ageing in all have booster action to sea urchin peptide.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated:
Following examples sea urchin place of production used is Weihai in Shandong province, and (enzyme activity is 1 × 10 to food-grade lipase used
4u/g) (enzyme activity is 3 × 10 with bromeline
5u/g) Dong Henghua road, Nanning bio tech ltd is all purchased; (enzyme activity is 1 × 10 to Ginger Protease
5u/g) purchased from Zhengzhou Kang Feng thing science and technology.
embodiment 1
(1) after being cleaned by the new urchin of select, pry open the soft shell of the radial prickle of black bands with scissors, dig out the sea urchin egg like orange lobe yellow of shape in shell with soup spoon, remove internal organ, take 2000g, add the deionized water of 12 ~ 16L, defibrination, be gonad of Hemicentrotus seu Strongylocentrotus slurry, gonad of Hemicentrotus seu Strongylocentrotus slurry be placed in the enzymatic vessel of 20L, stir, in chuck, pass into steam be warming up to 70 ~ 80 DEG C, maintain 10 ~ 15min, be then cooled to 45 ± 1 DEG C, adjust ph is to pH8 ~ 10;
(2) lipase (1 × 10 of 20 ~ 40g is added
4u/g), in 45 ± 1 DEG C of constant temperature hydrolysis, 3 ~ 4h under 20 ~ 30rpm stirs, its effect is lipid acid and glycerine by the fat hydrolysis in gonad of Hemicentrotus seu Strongylocentrotus, and lipid acid and alkali form soap salt, make it soluble in water, proceed to aqueous phase;
(3), after enzymolysis terminates, enzymolysis solution adopts and blocks molecular weight is that the ultra-filtration membrane of 10000Da carries out ultrafiltration, and pressure reduction is 0.02MP, concentration ratio 5, collects trapped fluid, obtains gonad of Hemicentrotus seu Strongylocentrotus crude protein solution.
(4) the gonad of Hemicentrotus seu Strongylocentrotus crude protein solution will collected, after adding 6 ~ 8L deionized water, proceeds in 20L enzymatic vessel, stir, in chuck, pass into steam be warming up to 90 ~ 100 DEG C, maintain 10 ~ 15min, make the sex change of gonad of Hemicentrotus seu Strongylocentrotus albumen appropriateness, be easy to enzymolysis, be then cooled to 45 ~ 50 DEG C;
(5) 35 ~ 40g bromeline is added, in 45 ~ 50 DEG C of enzymolysis 2 ~ 3h under 20 ~ 30rpm stirs; Getting sucrose 3 ~ 3.5g joins in enzymolysis solution, fully dissolves after mixing, and adds under 1 ~ 2g Ginger Protease, 20 ~ 30rpm stirs and continues enzymolysis 3 ~ 4h in 45 ~ 50 DEG C;
(6) after enzymolysis terminates, in chuck, pass into steam be warming up to 90 ~ 100 DEG C, maintain 10 ~ 15min, make proteolytic enzyme deactivation, after being cooled to 30 ~ 40 DEG C, enzymolysis solution is released from enzymatic vessel;
(7) enzymolysis solution of releasing is carried out suction filtration, make the enzymolysis solution containing a small amount of impurity pass through to be covered with the thick diatomite filter of 4 ~ 6mm in advance, obtain clear filtrate; Adopting and blocking molecular weight is that the ultra-filtration membrane of 2000Da carries out ultrafiltration, permeate is in 70 ~ 80 DEG C, vacuum concentration 3 ~ 4 times under the condition of vacuum tightness 0.06 ~ 0.08MPa, mix with fructus lycii extracted solution, hawthorn extract again, add 0.5 ~ 0.75g nisin, after fully dissolving, be sea urchin peptide oral liquid;
(8) preparation of described fructus lycii extracted solution: take matrimony vine 120 ~ 130g, after deionized water rinsing, adds 0.5 ~ 0.6L deionized water, soak 2 ~ 3h, boiling 20 ~ 30min, two-layer gauze coarse filtration, collects filtrate and residue respectively, residue adds 0.4 ~ 0.5L deionized water, boiling 15 ~ 20min, after two-layer filtered through gauze, merges the filtrate obtained for twice, adopt the Büchner funnel being covered with the thick diatomite filter of 4 ~ 6mm in advance to carry out suction filtration, clarification permeate is fructus lycii extracted solution;
(9) preparation of described hawthorn extract: take hawthorn 120 ~ 130g, stoning, clean, add 0.5 ~ 0.6L deionized water, soak 2 ~ 3h, colloidal mill is pulled an oar, hawthorn slurry boiling 20 ~ 30min, adopt the Büchner funnel being covered with the thick diatomite filter of 4 ~ 6mm in advance to carry out suction filtration, clarification permeate is hawthorn extract;
(10) the sea urchin peptide solution clear produced of the present embodiment, light yellow to yellow, have the distinctive sweet taste of sea urchin and delicate flavour, its physical and chemical index is as follows: total nitrogen 0.5 ~ 0.6g/100g, peptide content 2.2 ~ 2.6g/100g, relative molecular mass is less than ratio >=80% of the peptide section of 2000Da.
embodiment 2
(1) after being cleaned by the new urchin of select, pry open the soft shell of the radial prickle of black bands with scissors, dig out the sea urchin egg like orange lobe yellow of shape in shell with soup spoon, remove internal organ, take 2000g, add the deionized water of 12 ~ 16L, defibrination, be gonad of Hemicentrotus seu Strongylocentrotus slurry, gonad of Hemicentrotus seu Strongylocentrotus slurry be placed in the enzymatic vessel of 20L, stir, in chuck, pass into steam be warming up to 70 ~ 80 DEG C, maintain 10 ~ 15min, be then cooled to 45 ± 1 DEG C, adjust ph is to pH8 ~ 10;
(2) lipase (1 × 10 of 20 ~ 40g is added
4u/g), in 45 ± 1 DEG C of constant temperature hydrolysis, 3 ~ 4h under 20 ~ 30rpm stirs, its effect is lipid acid and glycerine by the fat hydrolysis in gonad of Hemicentrotus seu Strongylocentrotus, and lipid acid and alkali form soap salt, make it soluble in water, proceed to aqueous phase;
(3), after enzymolysis terminates, enzymolysis solution adopts and blocks molecular weight is that the ultra-filtration membrane of 10000Da carries out ultrafiltration, and pressure reduction is 0.02MP, concentration ratio 5, collects trapped fluid, obtains gonad of Hemicentrotus seu Strongylocentrotus crude protein solution;
(4) the gonad of Hemicentrotus seu Strongylocentrotus crude protein solution will collected, after adding 6 ~ 8L deionized water, proceed in 20L enzymatic vessel, stir, in chuck, pass into steam be warming up to 90 ~ 100 DEG C, maintain 10 ~ 15min, make the sex change of gonad of Hemicentrotus seu Strongylocentrotus albumen appropriateness, be easy to enzymolysis, then 45 ~ 50 DEG C are cooled to, add fructus lycii extracted solution, mix, make anti-oxidation active substance available protecting protease activity in fructus lycii extracted solution;
(5) preparation of described fructus lycii extracted solution: take matrimony vine 120 ~ 130g, after deionized water rinsing, adds 0.5 ~ 0.6L deionized water, soak 2 ~ 3h, boiling 20 ~ 30min, two-layer gauze coarse filtration, collects filtrate and residue respectively, residue adds 0.4 ~ 0.5L deionized water, boiling 15 ~ 20min, after two-layer filtered through gauze, merges the filtrate obtained for twice, adopt the Büchner funnel being covered with the thick diatomite filter of 4 ~ 6mm in advance to carry out suction filtration, clarification permeate is fructus lycii extracted solution;
(6) 35 ~ 40g bromeline is added, in 45 ~ 50 DEG C of enzymolysis 2 ~ 3h under 20 ~ 30rpm stirs; Getting sucrose 3 ~ 3.5g joins in enzymolysis solution, fully dissolves after mixing, and adds under 1 ~ 2g Ginger Protease, 20 ~ 30rpm stirs and continues enzymolysis 3 ~ 4h in 45 ~ 50 DEG C;
(7) after enzymolysis terminates, in chuck, pass into steam be warming up to 90 ~ 100 DEG C, maintain 10 ~ 15min, make proteolytic enzyme deactivation, after being cooled to 30 ~ 40 DEG C, enzymolysis solution is released from enzymatic vessel;
(8) enzymolysis solution of releasing is carried out suction filtration, the enzymolysis solution containing a small amount of impurity is made to pass through to be covered with the thick diatomite filter of 4 ~ 6mm in advance, obtain clear filtrate, adopting and blocking molecular weight is that the ultra-filtration membrane of 2000Da carries out ultrafiltration, permeate is in 70 ~ 80 DEG C, vacuum concentration 3 ~ 4 times under the condition of vacuum tightness 0.06 ~ 0.08MPa, mix with hawthorn extract again, add 0.5 ~ 0.75g nisin, after fully dissolving, be sea urchin peptide oral liquid;
(9) preparation of described hawthorn extract: take hawthorn 120 ~ 130g, stoning, clean, add 0.5 ~ 0.6L deionized water, soak 2 ~ 3h, colloidal mill is pulled an oar, hawthorn slurry boiling 20 ~ 30min, adopt the Büchner funnel being covered with the thick diatomite filter of 4 ~ 6mm in advance to carry out suction filtration, clarification permeate is hawthorn extract;
(10) the sea urchin peptide solution clear produced of the present embodiment, light yellow to yellow, have the distinctive sweet taste of sea urchin and delicate flavour, its physical and chemical index is as follows: total nitrogen 0.55 ~ 0.65g/100g, peptide content 2.4 ~ 2.8g/100g, relative molecular mass is less than ratio >=85% of the peptide section of 2000Da.
The invention has the beneficial effects as follows:
1, the present invention uses prozyme to prepare sea urchin peptide, prozyme used is plant protease, particularly Ginger Protease, extract from ginger, ginger is the vegetables of dietotherapeutic, aboundresources, therefore uses Ginger Protease to produce sea urchin peptide oral liquid not only economical but also ensure that food safety;
2, the present invention adopts prozyme to be hydrolyzed the mode preparing sea urchin peptide stage by stage, first bromeline is adopted to carry out enzymolysis, NaCl in gonad of Hemicentrotus seu Strongylocentrotus has certain provide protection to its activity, then adopt Ginger Protease to continue enzymolysis, and use sucrose as its enzyme activator alive, collaborative bromelain hydrolyzate gonad of Hemicentrotus seu Strongylocentrotus albumen, not only increase degree of hydrolysis, increase polypeptide and free aminoacid content, and enhance the delicate flavour of sea urchin peptide oral liquid, be more conducive to human body digesting and assimilating food;
3, fructus lycii extracted solution and hawthorn extract and sea urchin peptide solution are allocated by the present invention, and do not add any chemical substance, make sea urchin peptide oral liquid mouthfeel pure and honest, sour-sweet moderate, delicate flavour is pure.Abundant lycium barbarum polysaccharide, organic acid, glycoside and Flavonoid substances is contained in matrimony vine and hawthorn extract, its anti-oxidant activity substantially increases the stability of sea urchin peptide oral liquid, extend the shelf life, at immunity moderation, protect the liver, antitumor, anti-ageing in all have booster action to sea urchin peptide.
Claims (8)
1. a sea urchin peptide oral liquid, is characterized in that removing after fat by fresh gonad of Hemicentrotus seu Strongylocentrotus through lipase hydrolysis, adopts Ginger Protease and bromeline to be hydrolyzed and is prepared from.
2. a kind of combined-enzyme method prepares the method for sea urchin peptide according to claim 1, it is characterized in that concrete steps are as follows:
New urchin pushes shell aside, gills, and takes out sexual gland, is gonad of Hemicentrotus seu Strongylocentrotus, carries out enzymic hydrolysis except degrease, obtain gonad of Hemicentrotus seu Strongylocentrotus crude protein after ultrafiltration with lipase;
Gonad of Hemicentrotus seu Strongylocentrotus crude protein mixes with deionized water, making beating, and heating, makes gonad of Hemicentrotus seu Strongylocentrotus protein denaturation, adopts Ginger Protease and bromelain hydrolyzate after cooling;
Enzymolysis solution through the enzyme that goes out, filtration, ultrafiltration, allotment and concentrated after, obtain sea urchin peptide oral liquid.
3. combined-enzyme method according to claim 2 prepares the method for sea urchin peptide, it is characterized in that step (1) concrete operations are: get new urchin, push shell aside, by removals such as internal organ, take out sexual gland, gonad of Hemicentrotus seu Strongylocentrotus and deionized water 1:6 ~ 8 are mixed and pulls an oar, be heated to 70 ~ 80 DEG C and maintain 10 ~ 15min, to 40 ± 1 DEG C after cooling, add food-grade lipase by the consumption of 100 ~ 200u/g gonad of Hemicentrotus seu Strongylocentrotus, in pH8 ~ 10, the Water Under solution 2 ~ 4h of 40 ± 1 DEG C; Enzymolysis solution adopts and blocks molecular weight is that the ultra-filtration membrane of 10000Da carries out ultrafiltration and concentration, and cycles of concentration is 2 ~ 3 times, and trapped fluid is gonad of Hemicentrotus seu Strongylocentrotus crude protein solution.
4. combined-enzyme method according to claim 2 prepares the method for sea urchin peptide, it is characterized in that step (2) concrete operations are: mixed with the deionization of 2 ~ 3 times of volumes by gonad of Hemicentrotus seu Strongylocentrotus crude protein solution, be heated to 90 ~ 100 DEG C and maintain 10 ~ 15min, be cooled to 45 ~ 50 DEG C, add 3 ~ 3.5g/L sucrose, after dissolving, add Ginger Protease and bromeline carries out enzymolysis; Zingibain enzyme dosage is 50 ~ 100u/g gonad of Hemicentrotus seu Strongylocentrotus, and the consumption of bromeline is 5800 ~ 6200u/g gonad of Hemicentrotus seu Strongylocentrotus; Enzymatic hydrolysis condition is 45 ~ 50 DEG C, and pH nature, hydrolysis time is 4 ~ 6h.
5. combined-enzyme method according to claim 2 prepares the method for sea urchin peptide, it is characterized in that, concrete operations described in step (3) are: enzymolysis solution carries out going out enzyme in 90 ~ 100 DEG C of insulation 10min, then adopt and be covered with the thick diatomite filter of 4 ~ 6mm in advance and carry out suction filtration, it is that the ultra-filtration membrane of 2000Da carries out ultrafiltration that filtrate employing blocks molecular weight, permeate carries out vacuum concentration in 70 ~ 80 DEG C, vacuum tightness is 0.06 ~ 0.08MPa, cycles of concentration is 3 ~ 4 times, add fructus lycii extracted solution, hawthorn extract and nisin again to allocate, obtain sea urchin peptide oral liquid.
6. combined-enzyme method according to claim 5 prepares the method for sea urchin peptide, it is characterized in that described fructus lycii extracted solution preparation method is: the consumption of matrimony vine is 60 ~ 65g/kg gonad of Hemicentrotus seu Strongylocentrotus, after rinsing well, deionized water is added in the ratio of 1:3 ~ 4, soak 2 ~ 3h, boiling 20 ~ 30min, two-layer gauze coarse filtration, collect filtrate and residue respectively, residue is pressed 1:2 ~ 3 and is added deionized water, boiling 15 ~ 20min, after two-layer filtered through gauze, merge the filtrate obtained for twice, the Büchner funnel being covered with the thick diatomite filter of 4 ~ 6mm is in advance adopted to carry out suction filtration, clarification permeate is fructus lycii extracted solution.
7. combined-enzyme method according to claim 5 prepares the method for sea urchin peptide, it is characterized in that described hawthorn extract preparation method is: the consumption of hawthorn is 60 ~ 65g/kg gonad of Hemicentrotus seu Strongylocentrotus, and hawthorn stoning, after deionized water rinsing, deionized water is added in 1:3 ~ 4, soak 2 ~ 3h, colloidal mill is pulled an oar, hawthorn slurry boiling 20 ~ 30min, two-layer gauze coarse filtration, collect filtrate, adopt the Büchner funnel being covered with the thick diatomite filter of 4 ~ 6mm in advance to carry out suction filtration, clarification permeate is hawthorn extract.
8. combined-enzyme method according to claim 5 prepares the method for sea urchin peptide, it is characterized in that the consumption of described nisin is 0.15 ~ 0.18g/kg.
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| CN107320498A (en) * | 2017-07-12 | 2017-11-07 | 熊廷珍 | The sea urchin polysaccharide gel patch postoperative for Humanmachine tumour |
| CN107353416A (en) * | 2017-07-12 | 2017-11-17 | 熊廷珍 | A kind of HPV suppresses the preparation method of gel |
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