CN105063151B - Sea urchin peptide oral liquid and preparation method thereof - Google Patents
Sea urchin peptide oral liquid and preparation method thereof Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of sea urchin peptide oral liquids and preparation method thereof, it is characterized by the following steps: after new urchin peels off, take out sea urchin Huang and water mixing defibrination, lipase is added and carries out enzymatic hydrolysis degreasing, trapped fluid, as sea urchin Huang crude protein solution are collected in ultrafiltration, sea urchin Huang crude protein solution is used into bromelain and Ginger Protease stage coordinated enzymatic hydrolysis, enzymolysis liquid obtains sea urchin peptide oral liquid after allotment and concentration, this method effectively prevents the rancid phenomenon of sea urchin Huang using filter, ultrafiltration；The safety of food has been effectively ensured in the utilization rate for substantially increasing sea urchin protein；Not only improve the mouthfeel of sea urchin peptide oral liquid, antioxidant activity substantially increases the stability of sea urchin peptide oral liquid, extends the shelf-life, and have booster action to sea urchin peptide in terms of adjusting immune, liver protection, antitumor, anti-aging.
Description
Technical field
The present invention relates to technical field of bioengineering, specifically a kind of sea urchin peptide oral liquid and preparation method thereof.
Background technique
The name of sea urchin is that one kind eats extensive marine fishery all over the world first recorded in " book on Chinese herbal medicine is original ".The life of sea urchin
Growing gland is sea urchin Huang, containing a large amount of protein, amino acid, highly unsaturated fatty acid, carbohydrate and some physiological activators,
Not only there is very high edible value, there are also fabulous medicinal functions, have preferable control efficiency to cardiovascular disease.The Liao Dynasty of China
Rather, always there is the habit for eating sea urchin on the ground such as Shandong, Guangdong, Fujian and Hainan.Japan, Malay Archipelago, South America and Mediterranean
Some countries, such as France, Italy, Greece country also there is the habit for eating sea urchin, especially japanese people especially to have a liking for eating
Sea urchin.It can be seen that sea urchin is a kind of very high marine resources of nutritive value, space is had a vast market, the research to it
It has a very important significance.
The fresh-keeping and storage problem of sea urchin is one of the main restricting factor of sea urchin industry development.Sea urchin Huang is easy self-dissolving,
After sea urchin fishing water outlet, place half to one, it is possible to feel like jelly rotten, lose edible value.Deep processing is carried out to sea urchin, no
But its shelf-life can be extended, can also promote its resource value.Currently, sea urchin deep processed product in the market mainly has sea urchin
Sauce, sea urchin gruel, alcohol sea urchin.Sea urchin protein is predominantly in the form of macro-molecular protein in these products, is unfavorable for human body
Be absorbed and utilized.Sea urchin protein is such as carried out by appropriate hydrolysis using protease, generation is with the small peptide that 2--6 amino acid forms
The sea urchin peptide of main component can directly be absorbed by human body without digestion.Compared with sea urchin protein, sea urchin peptide not only has higher
Nutritive value, but also have it is anti-oxidant, antitumor, improve the physiological functions such as body immunity.The present invention uses complex enzyme
Method carries out appropriate hydrolysis to sea urchin and prepares sea urchin peptide oral liquid, is not only more convenient to eat, but also sea urchin protein is hydrolyzed into small point
Sub- active peptide improves its nutritive value and absorption rate.
Summary of the invention
Present invention aim to address above-mentioned the deficiencies in the prior art, provide a kind of nutritive value is high, absorption rate is high,
Mouthfeel is pure and honest, sour-sweet moderate, irresistible sea urchin peptide oral liquid and preparation method thereof.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of sea urchin peptide oral liquid, it is characterised in that after new urchin ecliptic longitude degreasing, be prepared using combined-enzyme method.
The preparation method of sea urchin peptide oral liquid of the present invention is to carry out water using lipase first using sea urchin Huang as raw material
Solution obtains sea urchin Huang crude protein solution after ultrafiltration, then carries out appropriate hydrolysis using compound protease, makes sea urchin Huang proteolysis
To molecular weight 2000Da hereinafter, using processes such as filter, concentration, allotments, sea urchin peptide oral liquid is obtained.
Specific step is as follows for sea urchin peptide oral liquid of the present invention and preparation method thereof:
(1) new urchin is pushed aside, takes out gonad, is i.e. sea urchin is yellow；
(2) sea urchin Huang is mixed into defibrination with deionized water, lipase is added and is digested, ultrafiltration collects trapped fluid, as
Sea urchin Huang crude protein solution；
(3) sea urchin Huang crude protein solution is mixed with deionized water, is stirred evenly, heated, be denaturalized it moderately, be added after cooling
Complex enzyme is digested；
(4) after digesting, enzymolysis liquid is subjected to enzyme deactivation, filters, be concentrated to get sea urchin peptide solution；
(5) fructus lycii extracted solution, hawthorn extract, nisin are added into sea urchin peptide solution, is deployed, obtains
Sea urchin peptide oral liquid.
Step (1) concrete operations of the present invention are as follows: after cleaning the new urchin of select, pry open black bands with scissors
The soft shell of radial prickle digs out shape in shell with soup spoon and removes internal organ like the sea urchin egg of orange valve yellow.
The concrete operations of step (2) of the present invention are as follows: sea urchin Huang is added into the uniformly mixed defibrination in water 1:6 ~ 8, obtains sea urchin
Huang 70~80 DEG C of 10~15min of heating of slurry, are subsequently cooled to 40 ± 1 DEG C, and lipase is added and is digested, after enzymatic hydrolysis, enzyme
Solution liquid uses truncation molecular weight to carry out ultrafiltration for the ultrafiltration membrane of 10000Da, and cycles of concentration is 2 ~ 3 times, collects trapped fluid, obtains sea
Jaundice due to fright or traumatic injury crude protein solution.
The dosage of lipase of the present invention is that 100~200u/g sea urchin is yellow, and enzymatic hydrolysis condition is pH8~10,40 ± 1 DEG C,
2~4h of time.
Step (3) concrete operations of the present invention are that the deionized water of sea urchin Huang crude protein solution and 2 ~ 3 times of volumes is mixed
It closes uniformly, is heated to 90~100 DEG C of 10~15min of maintenance, to 45 ~ 50 DEG C after cooling, food-grade bromelain, enzymatic hydrolysis is added
After 2 ~ 3h, 3 ~ 3.5g/L sucrose is added, sufficiently dissolution is uniformly mixed, and food-grade Ginger Protease is added, continues 3 ~ 4h of enzymatic hydrolysis,
The dosage of bromelain of the present invention is that 5800~6200u/g sea urchin is yellow, Zingibain enzyme dosage is 50~
100u/g sea urchin is yellow.
The concrete operations of step (4) of the present invention are as follows: after 90~100 DEG C of heating 10~15min enzyme deactivations of enzymolysis liquid, adopt
Be separated by solid-liquid separation with the method for filtering or centrifugation, remove a small amount of residue, obtain clarified solution, clarified solution use be truncated molecular weight for
The ultrafiltration membrane of 2000Da carries out ultrafiltration, and permeate is concentrated in vacuo in 70~80 DEG C, and vacuum degree is 0.06~0.08MPa, dense
Demagnification number is 3~4 times, as sea urchin peptide solution.
Fructus lycii extracted solution concrete operations are prepared in step (5) of the present invention are as follows: press the use of 60 ~ 65g/kg of fructus lycii sea urchin Huang
Amount weighs fructus lycii, and after deionized water is rinsed, 1:3 ~ 4 adds deionized water, impregnates 2 ~ 3h, 20 ~ 30min of boiling, and two layers of gauze is thick
Filtering, collects filtrate and residue respectively, and residue adds deionized water by 1:2 ~ 3,15 ~ 20min of boiling, after two layers of filtered through gauze,
Merge the filtrate obtained twice, filtered using the Buchner funnel for the diatomite filter for being covered with 4~6mm thickness in advance, clarification penetrates
Liquid is fructus lycii extracted solution.
It is the use by 60 ~ 65g/kg of hawthorn sea urchin Huang that hawthorn extract concrete operations are prepared in step (5) of the present invention
Amount weighs hawthorn, is enucleated, and after deionized water is rinsed, 1:3 ~ 4 adds deionized water, impregnates 2 ~ 3h, colloid mill mashing, and hawthorn slurry steams
Boil 20 ~ 30min, filtrate is collected in two layers of gauze coarse filtration, using the diatomite filter for being covered with 4~6mm thickness in advance Buchner funnel into
Row filters, and clarification permeate is hawthorn extract.
The dosage of nisin is 0.15 ~ 0.18g/kg sea cucumber peptide oral liquid in step (5) of the present invention
Step (5) concrete operations of the present invention are as follows: by fructus lycii extracted solution, hawthorn extract and nisin with
Sea urchin peptide solution is uniformly mixed, as sea urchin peptide oral liquid.
The beneficial effects of the present invention are:
1, the present invention prepares sea urchin peptide using complex enzyme, and complex enzyme used is plant rennet, especially Zingibain
Enzyme is extracted from ginger, and ginger is the vegetables of dietotherapeutic, resourceful, therefore produces sea urchin using Ginger Protease
Peptide oral liquid is not only economical but also ensure that foodsafety；
2, the present invention is by the way of using complex enzyme, hydrolysis prepares sea urchin peptide stage by stage, first using bromelain progress enzyme
It solves, the NaCl in sea urchin Huang has certain protective effect to its activity, then continues to digest using Ginger Protease, and use
Sucrose cooperates with bromelain hydrolyzate sea urchin Huang albumen as its enzyme activity activator, not only increases degree of hydrolysis, increase polypeptide and
Free aminoacid content, and the delicate flavour of sea urchin peptide oral liquid is enhanced, it is more advantageous to digestion and absorption of the human body to food；
3, the present invention deploys fructus lycii extracted solution and hawthorn extract and sea urchin peptide solution, does not add any chemicals
Matter keeps sea urchin peptide oral liquid mouthfeel pure and honest, sour-sweet moderate, irresistible.Fructus lycii rich in fructus lycii and hawthorn extract
Polysaccharide, organic acid, glycoside and Flavonoid substances, antioxidant activity substantially increase the stability of sea urchin peptide oral liquid, extend
There is booster action in shelf-life to sea urchin peptide in terms of adjusting immune, liver protection, antitumor, anti-aging.
Specific embodiment
Below with reference to embodiment, the present invention is further illustrated:
The sea urchin place of production used in following embodiment is Weihai in Shandong province, and (enzyme activity is 1 × 10 to food-grade lipase used4U/g)
(enzyme activity is 3 × 10 with bromelain5U/g the road Nanning Dong Henghua Biotechnology Co., Ltd) is purchased；Ginger Protease
(enzyme activity is 1 × 105U/g) purchased from Zhengzhou Kang Feng object science and technology.
Embodiment 1
(1) after cleaning the new urchin of select, the soft shell of the radial prickle of black bands is pried open with scissors, is dug with soup spoon
Shape removes internal organ like the sea urchin egg of orange valve yellow in shell out, weighs 2000g, adds the deionized water of 12~16L, defibrination, i.e.,
For sea urchin Huang slurry, sea urchin Huang slurry is placed in the enzymatic vessel of 20L, stirs evenly, steam is passed through into collet and is warming up to 70~80
DEG C, 10~15min is maintained, is subsequently cooled to 45 ± 1 DEG C, adjusts pH value to pH8~10；
(2) lipase (1 × 10 of 20~40g is added4U/g), under 20~30rpm stirring in 45 ± 1 DEG C of constant temperature hydrolysis 3~
4h, it is fatty acid and glycerol that effect, which is by the grease hydrolysis in sea urchin Huang, and fatty acid forms soap salt with alkali, it is made to be dissolved in water
In, it is transferred to water phase；
(3) after digesting, enzymolysis liquid uses truncation molecular weight to carry out ultrafiltration for the ultrafiltration membrane of 10000Da, and pressure difference is
0.02MP, cocnentration factor 5 collect trapped fluid, obtain sea urchin Huang crude protein solution.
(4) the sea urchin Huang crude protein solution of collection is transferred in 20L enzymatic vessel after 6 ~ 8L deionized water is added, stirring is equal
It is even, it is passed through steam into collet and is warming up to 90~100 DEG C, maintains 10~15min, is denaturalized sea urchin Huang albumen moderately, is easy to enzyme
Solution, is subsequently cooled to 45 ~ 50 DEG C；
(5) 35~40g bromelain is added, in 45~50 DEG C of 2 ~ 3h of enzymatic hydrolysis under 20~30rpm stirring；Take sucrose 3 ~
3.5g is added in enzymolysis liquid, and sufficiently dissolution after mixing, is added under 1~2g Ginger Protease, 20~30rpm stirring in 45
~50 DEG C are continued 3 ~ 4h of enzymatic hydrolysis；
(6) after digesting, it is passed through steam into collet and is warming up to 90~100 DEG C, maintains 10~15min, makes protease
After being cooled to 30~40 DEG C, enzymolysis liquid is released from enzymatic vessel for deactivation；
(7) enzymolysis liquid of releasing is filtered, the enzymolysis liquid containing a small amount of impurity is made to pass through the silicon for being covered with 4~6mm thickness in advance
Diatomaceous earth filtering layer, obtains clear filtrate；Truncation molecular weight is used to carry out ultrafiltration for the ultrafiltration membrane of 2000Da, permeate is in 70~80
DEG C, 3~4 times are concentrated in vacuo under conditions of 0.06~0.08MPa of vacuum degree, then mixed with fructus lycii extracted solution, hawthorn extract
It is even, 0.5 ~ 0.75g nisin is added, after completely dissolution as sea urchin peptide oral liquid；
(8) preparation of the fructus lycii extracted solution: weighing 120 ~ 130g of fructus lycii, after deionized water is rinsed, adds 0.5 ~ 0.6L
Deionized water, impregnates 2 ~ 3h, 20 ~ 30min of boiling, two layers of gauze coarse filtration, collects filtrate and residue respectively, and residue addition 0.4 ~
0.5L deionized water, 15 ~ 20min of boiling after two layers of filtered through gauze, merge the filtrate obtained twice, using being covered with 4~6mm in advance
The Buchner funnel of thick diatomite filter is filtered, and clarification permeate is fructus lycii extracted solution；
(9) preparation of the hawthorn extract: weighing 120 ~ 130g of hawthorn, is enucleated, and cleans, and adds 0.5 ~ 0.6L deionization
Water impregnates 2 ~ 3h, and colloid mill mashing, hawthorn starches 20 ~ 30min of boiling, using the cloth for the diatomite filter for being covered with 4~6mm thickness in advance
Family name's funnel filters, and clarification permeate is hawthorn extract；
(10) the present embodiment sea urchin peptide solution clear produced, it is light yellow to yellow, there is the distinctive sweet tea of sea urchin
Taste and delicate flavour, physical and chemical index are as follows: 0.5 ~ 0.6g/100g of total nitrogen, 2.2 ~ 2.6g/100g of peptide content, and relative molecular mass is small
In ratio >=80% of the peptide fragment of 2000Da.
Embodiment 2
(1) after cleaning the new urchin of select, the soft shell of the radial prickle of black bands is pried open with scissors, is dug with soup spoon
Shape removes internal organ like the sea urchin egg of orange valve yellow in shell out, weighs 2000g, adds the deionized water of 12~16L, defibrination, i.e.,
For sea urchin Huang slurry, sea urchin Huang slurry is placed in the enzymatic vessel of 20L, stirs evenly, steam is passed through into collet and is warming up to 70~80
DEG C, 10~15min is maintained, is subsequently cooled to 45 ± 1 DEG C, adjusts pH value to pH8~10；
(2) lipase (1 × 10 of 20~40g is added4U/g), under 20~30rpm stirring in 45 ± 1 DEG C of constant temperature hydrolysis 3~
4h, it is fatty acid and glycerol that effect, which is by the grease hydrolysis in sea urchin Huang, and fatty acid forms soap salt with alkali, it is made to be dissolved in water
In, it is transferred to water phase；
(3) after digesting, enzymolysis liquid uses truncation molecular weight to carry out ultrafiltration for the ultrafiltration membrane of 10000Da, and pressure difference is
0.02MP, cocnentration factor 5 collect trapped fluid, obtain sea urchin Huang crude protein solution；
(4) the sea urchin Huang crude protein solution of collection is transferred in 20L enzymatic vessel after 6 ~ 8L deionized water is added, stirring is equal
It is even, it is passed through steam into collet and is warming up to 90~100 DEG C, maintains 10~15min, is denaturalized sea urchin Huang albumen moderately, is easy to enzyme
Solution is subsequently cooled to 45 ~ 50 DEG C, and fructus lycii extracted solution is added, and is uniformly mixed, keeps anti-oxidation active substance in fructus lycii extracted solution effective
Protected protein enzymatic activity；
(5) preparation of the fructus lycii extracted solution: weighing 120 ~ 130g of fructus lycii, after deionized water is rinsed, adds 0.5 ~ 0.6L
Deionized water, impregnates 2 ~ 3h, 20 ~ 30min of boiling, two layers of gauze coarse filtration, collects filtrate and residue respectively, and residue addition 0.4 ~
0.5L deionized water, 15 ~ 20min of boiling after two layers of filtered through gauze, merge the filtrate obtained twice, using being covered with 4~6mm in advance
The Buchner funnel of thick diatomite filter is filtered, and clarification permeate is fructus lycii extracted solution；
(6) 35~40g bromelain is added, in 45~50 DEG C of 2 ~ 3h of enzymatic hydrolysis under 20~30rpm stirring；Take sucrose 3 ~
3.5g is added in enzymolysis liquid, and sufficiently dissolution after mixing, is added under 1~2g Ginger Protease, 20~30rpm stirring in 45
~50 DEG C are continued 3 ~ 4h of enzymatic hydrolysis；
(7) after digesting, it is passed through steam into collet and is warming up to 90~100 DEG C, maintains 10~15min, makes protease
After being cooled to 30~40 DEG C, enzymolysis liquid is released from enzymatic vessel for deactivation；
(8) enzymolysis liquid of releasing is filtered, the enzymolysis liquid containing a small amount of impurity is made to pass through the silicon for being covered with 4~6mm thickness in advance
Diatomaceous earth filtering layer, obtains clear filtrate, uses truncation molecular weight to carry out ultrafiltration for the ultrafiltration membrane of 2000Da, permeate is in 70~80
DEG C, 3~4 times are concentrated in vacuo under conditions of 0.06~0.08MPa of vacuum degree, then be uniformly mixed with hawthorn extract, add 0.5 ~
0.75g nisin, after completely dissolution as sea urchin peptide oral liquid；
(9) preparation of the hawthorn extract: weighing 120 ~ 130g of hawthorn, is enucleated, and cleans, and adds 0.5 ~ 0.6L deionization
Water impregnates 2 ~ 3h, and colloid mill mashing, hawthorn starches 20 ~ 30min of boiling, using the cloth for the diatomite filter for being covered with 4~6mm thickness in advance
Family name's funnel filters, and clarification permeate is hawthorn extract；
(10) the present embodiment sea urchin peptide solution clear produced, it is light yellow to yellow, there is the distinctive sweet tea of sea urchin
Taste and delicate flavour, physical and chemical index are as follows: 0.55 ~ 0.65g/100g of total nitrogen, 2.4 ~ 2.8g/100g of peptide content, relative molecular mass
Ratio >=85% of peptide fragment less than 2000Da.
The beneficial effects of the present invention are:
1, the present invention prepares sea urchin peptide using complex enzyme, and complex enzyme used is plant rennet, especially Zingibain
Enzyme is extracted from ginger, and ginger is the vegetables of dietotherapeutic, resourceful, therefore produces sea urchin using Ginger Protease
Peptide oral liquid is not only economical but also ensure that foodsafety；
2, the present invention is by the way of using complex enzyme, hydrolysis prepares sea urchin peptide stage by stage, first using bromelain progress enzyme
It solves, the NaCl in sea urchin Huang has certain protective effect to its activity, then continues to digest using Ginger Protease, and use
Sucrose cooperates with bromelain hydrolyzate sea urchin Huang albumen as its enzyme activity activator, not only increases degree of hydrolysis, increase polypeptide and
Free aminoacid content, and the delicate flavour of sea urchin peptide oral liquid is enhanced, it is more advantageous to digestion and absorption of the human body to food；
3, the present invention deploys fructus lycii extracted solution and hawthorn extract and sea urchin peptide solution, does not add any chemicals
Matter keeps sea urchin peptide oral liquid mouthfeel pure and honest, sour-sweet moderate, irresistible.Fructus lycii rich in fructus lycii and hawthorn extract
Polysaccharide, organic acid, glycoside and Flavonoid substances, antioxidant activity substantially increase the stability of sea urchin peptide oral liquid, extend
There is booster action in shelf-life to sea urchin peptide in terms of adjusting immune, liver protection, antitumor, anti-aging.
Claims (4)
1. a kind of sea urchin peptide oral liquid, it is characterised in that after new urchin ecliptic longitude lipase hydrolysis removing fat, using pineapple
Protease is hydrolyzed stage by stage with Ginger Protease and is prepared, and specific step is as follows for preparation:
(1) take new urchin, push shell aside, internal organ etc. are removed, take out gonad, as sea urchin is yellow, by sea urchin Huang and go from
Sub- water 1:6 ~ 8 mixes mashing, is heated to 70~80 DEG C of 10~15min of maintenance, to 40 ± 1 DEG C after cooling, by the sea 100~200u/g
Food-grade lipase is added in the dosage of jaundice due to fright or traumatic injury, and 2 ~ 4h is hydrolyzed under conditions of pH8~10,40 ± 1 DEG C；Enzymolysis liquid is using truncation point
The ultrafiltration membrane that son amount is 10000Da is concentrated by ultrafiltration, and cycles of concentration is 2 ~ 3 times, and trapped fluid is sea urchin Huang crude protein solution；
(2) sea urchin Huang crude protein solution is uniformly mixed with the deionization of 2 ~ 3 times of volumes, be heated to 90~100 DEG C maintain 10~
15min is cooled to 45 ~ 50 DEG C, and bromelain is added, and after digesting 2~3h, 3 ~ 3.5g/L sucrose is added, dissolution mixes, then
Ginger Protease is added, continues 3~4h of enzymatic hydrolysis, wherein 5800~6200u/g of dosage sea urchin of bromelain is yellow, Zingibain
Enzyme dosage is that 50~100u/g sea urchin is yellow；Enzymatic hydrolysis condition is 45 ~ 50 DEG C, and pH is natural；
(3) enzymolysis liquid carries out enzyme deactivation in 90 ~ 100 DEG C of 10~15min of heat preservation, then using the diatomite filter for being covered with 4~6mm thickness in advance
Layer is filtered, and filtrate uses truncation molecular weight to carry out ultrafiltration for the ultrafiltration membrane of 2000Da, and permeate carries out true in 70~80 DEG C
Sky concentration, vacuum degree are 0.06~0.08MPa, and cycles of concentration is 3 ~ 4 times, obtain sea urchin peptide solution；
(4) fructus lycii extracted solution, hawthorn extract and nisin are added into sea urchin peptide solution, is deployed, obtains sea
Gallbladder peptide oral liquid.
2. a kind of sea urchin peptide oral liquid according to claim 1, it is characterised in that the fructus lycii extracted solution preparation method
Are as follows: the dosage of fructus lycii is that 60 ~ 65g/kg sea urchin is yellow, after rinsing well, adds deionized water in the ratio of 1:3 ~ 4, impregnates 2 ~ 3h,
Filtrate and residue are collected in 20 ~ 30min of boiling, two layers of gauze coarse filtration respectively, and residue adds deionized water, boiling 15 by 1:2 ~ 3
~ 20min after two layers of filtered through gauze, merges the filtrate obtained twice, using the Bu Shi for the diatomite filter for being covered with 4~6mm thickness in advance
Funnel is filtered, and clarification permeate is fructus lycii extracted solution.
3. a kind of sea urchin peptide oral liquid according to claim 1, it is characterised in that the hawthorn extract preparation method
Are as follows: the dosage of hawthorn is that 60 ~ 65g/kg sea urchin is yellow, and hawthorn stoning, after deionized water is rinsed, 1:3 ~ 4 adds deionized water, is impregnated
2 ~ 3h, colloid mill mashing, hawthorn starch 20 ~ 30min of boiling, and filtrate is collected in two layers of gauze coarse filtration, thick using 4~6mm is covered in advance
The Buchner funnel of diatomite filter filtered, clarification permeate is hawthorn extract.
4. a kind of sea urchin peptide oral liquid according to claim 1, it is characterised in that the dosage of the nisin is
0.15~0.18g/kg。
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