CN105779542A - Method for preparing organic rapeseed polypeptide through enzyme method - Google Patents
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Abstract
The invention discloses a method for preparing organic rapeseed polypeptide through an enzyme method. The method includes the steps that rapeseed protein is prepared; the rapeseed protein and distilled water are mixed evenly according to a certain material-liquid ratio, and ultrasonic auxiliary heat-moisture treatment is performed to obtain heat-moisture rapeseed protein paste; sodium tripolyphosphate is added into the heat-moisture rapeseed protein paste for ultrasonic auxiliary phosphorylation treatment to obtain phosphorylated rapeseed protein paste; the pH value and temperature of the phosphorylated rapeseed protein paste are adjusted, and alkaline protease is added for ultrasonic auxiliary enzymolysis to obtain primary enzymatic hydrolysate; the pH value and temperature of primary enzymatic hydrolysate are adjusted, and composite flavored proteinase is added for ultrasonic auxiliary enzymolysis to obtain secondary enzymatic hydrolysate; microwave enzyme-killing treatment is performed on secondary enzymatic hydrolysate to obtain enzyme-killing enzymatic hydrolysate, the enzyme-killing enzymatic hydrolysate is centrifuged to obtain rapeseed polypeptide clear liquid, and then the rapeseed polypeptide clear liquid is subjected to freeze drying treatment to obtain the finished product organic rapeseed polypeptide. The method for preparing the organic rapeseed polypeptide through the enzyme method is safe in technology, and preparation is convenient and rapid.
Description
Technical field
The present invention relates to rapeseed peptides technical field, be specifically related to a kind of method that enzyme process prepares organic rapeseed peptides.
Background technology
Semen Brassicae campestris is a kind of important oil crop, and China occupies world-class rapeseed product big country, and Semen Brassicae campestris resource is very abundant, the cultivated area of Semen Brassicae campestris more than 100,000,000 mu, yield more than up to 1,300 ten thousand tons, occupies first of the world, and the rapeseed meal produced in the annual course of processing reaches more than 600 ten thousand tons.Rich in protein in Semen Brassicae campestris, reaches 25～27%, by protein content in the rapeseed meal after leaching oil removing up to 35～45%.In addition, rapeseed protein is a kind of high-quality protein, its aminoacid composition and content meet FAO/WHO and recommend mode value, there's almost no limiting amino acid, nutrition can compare favourably with casein and animal proteinum, compared with the other plant albumen such as Semen sojae atricolor, Semen arachidis hypogaeae, Semen Gossypii, its biological value and nutritive value are higher, are a kind of quality plant protein with potentiality to be exploited.Rapeseed protein being degraded into peptides, can improve utilization rate and the economic worth of rapeseed protein, these peptides have the advantage being more beneficial for human consumption and absorption than albumen, and have certain biological activity, for instance regulate immunity, blood pressure lowering and antioxidation etc..Therefore, it is necessary to study a kind of method preparing organic rapeseed peptides.
Summary of the invention
For the weak point existed in above-mentioned technology, the invention provides a kind of process safety, prepare the method that enzyme process easily prepares organic rapeseed peptides.
The technical solution adopted for the present invention to solve the technical problems is: a kind of enzyme process prepares the method for organic rapeseed peptides, including the preparation of: step one, rapeseed protein；Step 2, humid heat treatment: by rapeseed protein with distilled water by the mixing of certain solid-liquid ratio, and carry out ultrasonic wave added humid heat treatment, obtain damp and hot rapeseed protein serosity；Step 3, phosphatizing treatment: in damp and hot rapeseed protein serosity, add sodium tripolyphosphate carry out ultrasonic wave added phosphatizing treatment, treat that phosphatizing treatment terminates, obtain phosphorylation rapeseed protein serosity through dialysis；Step 4, primary enzymolysis: regulate the pH of phosphorylation rapeseed protein serosity and temperature, and add alkaline protease and carry out ultrasonic wave added enzymolysis, obtain primary enzymolysis liquid；Step 5, secondary enzymolysis: regulate the pH of primary enzymolysis liquid and temperature, and add compound protease and carry out ultrasonic wave added enzymolysis, obtain secondary enzymolysis liquid；Step 6, microwave deactivating enzyme: secondary enzymolysis liquid is carried out microwave deactivating enzyme process, obtain enzyme denaturing enzymolysis solution, obtains rapeseed peptides clear liquid by centrifugal for enzyme denaturing enzymolysis solution, then rapeseed peptides clear liquid is carried out lyophilization process, the organic rapeseed peptides got product.
Preferably, rapeseed meal powder raw material is carried out detoxification treatment, obtain virus-free rapeseed meal powder, virus-free rapeseed meal powder is made rapeseed meal powder serosity with distilled water by the mixing of solid-liquid ratio 1:12～15, regulate rapeseed meal powder serosity pH to 11～12, and carrying out ultrasonic wave added alkali-soluble process 1～2 hour, alkali-soluble temperature is 45～55 DEG C, and ultrasound intensity is 1.0～1.2W/cm3Treat that alkali-soluble process terminates, it is centrifuged and obtains the supernatant, adjustment supernatant pH to 4.5 carries out the heavy process of acid 1.0～1.5 hours, treats that the heavy process of acid terminates, is centrifuged and obtains precipitation of sinking, lower sediment is washed to neutrality, and carry out lyophilization process, and namely obtaining rapeseed protein, the water absorption of described rapeseed protein is 1.03～4.21g/g, oil absorption be 0.52～2.37g/g, emulsibility is 32.4～59.4%.
Preferably, the preparation process of described rapeseed meal powder raw material is as follows: adopting normal hexane to soak after being peeled by Semen Allii Tuberosi, obtain defat rapeseed meal after desolvation, its resid amount is 0.7～0.9%, and defat rapeseed meal was pulverized 40～80 mesh sieves, obtains rapeseed meal powder raw material.
Preferably, the process of described detoxification treatment is as follows: mixed by solid-liquid ratio 10～15:1 by the acetone that rapeseed meal powder raw material and concentration are 70%～80%, and carrying out ultrasound-assisted extraction 15～25 minutes, extraction temperature is 45～50 DEG C, and ultrasound intensity is 1.2～1.4W/cm3, end to be extracted, dry and be extracted rapeseed meal powder；Mixing extracting rapeseed meal powder by solid-liquid ratio 8～12:1 with the ethanol that concentration is 80%～90%, and carry out ultrasound-assisted extraction 20～30 minutes, extraction temperature is 60～70 DEG C, and ultrasound intensity is 1.4～1.6W/cm3, end to be extracted, dry and obtain virus-free rapeseed meal powder.
Preferably, in described step 2, the process of humid heat treatment is as follows: rapeseed protein and distilled water being pressed the mixing of solid-liquid ratio 1:8～12, and carries out ultrasonic wave added humid heat treatment 20～30 minutes, humid heat treatment temperature is 80～90 DEG C, and ultrasound intensity is 1.0～1.2W/cm3, obtain damp and hot rapeseed protein serosity.
Preferably, in described step 3, the process of phosphatizing treatment is as follows: adds sodium tripolyphosphate in damp and hot rapeseed protein serosity and carries out ultrasonic wave added phosphatizing treatment 1～1.5 hour, the addition of sodium tripolyphosphate is 1.5～2.5 times of damp and hot rapeseed protein serosity consumption, phosphatizing treatment temperature is 40～50 DEG C, and ultrasound intensity is 1.2～1.4W/cm3, treat that phosphatizing treatment terminates, obtain phosphorylation rapeseed protein serosity through dialysis.
Preferably, in described step 4, the process of primary enzymolysis is as follows: regulate the pH to 8～10 of phosphorylation rapeseed protein serosity, and add alkaline protease and carry out ultrasonic wave added enzymolysis, the addition of alkaline protease is the 4.5～5.5% of rapeseed protein quality, enzyme digestion reaction temperature is 50～60 DEG C, the enzyme digestion reaction time is 1～3 hour, and ultrasound intensity is 1.4～1.6W/cm3, treat that primary enzymolysis reaction terminates, obtain primary enzymolysis liquid.
Preferably, in described step 5, the process of secondary enzymolysis is as follows: regulate the pH to 5～7 of primary enzymolysis liquid, and add compound protease and carry out ultrasonic wave added enzymolysis, the addition of compound protease is the 4.5～5.5% of rapeseed protein quality, enzyme digestion reaction temperature is 45～55 DEG C, the enzyme digestion reaction time is 2～4 hours, and ultrasound intensity is 1.2～1.4W/cm3, treat that secondary enzymolysis reaction terminates, obtain secondary enzymolysis liquid.
Preferably, in described step 6, the process of microwave deactivating enzyme is as follows: secondary enzymolysis liquid is carried out microwave deactivating enzyme process, and microwave power is 500～800W, microwave deactivating enzyme temperature is 80～90 DEG C, the microwave deactivating enzyme time is 2～3 minutes, treats that microwave deactivating enzyme terminates, and obtains enzyme denaturing enzymolysis solution；Enzyme denaturing enzymolysis solution is centrifuged, take supernatant and obtain rapeseed peptides clear liquid, again rapeseed peptides clear liquid is carried out lyophilization process, the organic rapeseed peptides got product, the water absorption of described organic rapeseed peptides is 0.74～1.46g/g, oil absorption be 1.13～1.78g/g, foaming characteristic is 38.4～50.6%.
The present invention is compared with prior art, it provides the benefit that: enzyme process provided by the invention prepares the method for organic rapeseed peptides, by rapeseed meal powder raw material successively adopts acetone and ethanol carry out ultrasound on extracting, can effectively reduce the content of the noxious substances such as thioglycoside, phytic acid, polyphenol in rapeseed meal powder raw material；By rapeseed protein being sequentially carried out damp and hot and phosphatizing treatment, the efficiency of follow-up primary enzymolysis and secondary enzymolysis can be effectively improved；By adopting alkaline protease and compound protease that rapeseed protein is carried out ultrasonic wave added enzymolysis successively, the percent hydrolysis of rapeseed protein can be effectively improved；By adopting microwave deactivating enzyme, rapeseed peptides can be prevented effectively from and at high temperature inactivate；This enzyme process prepares the method for organic rapeseed peptides, process safety, prepare convenient.
Accompanying drawing explanation
Fig. 1 is the process chart that enzyme process of the present invention prepares the method for organic rapeseed peptides.
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is described in further detail, to make those skilled in the art can implement according to this with reference to description word.
As it is shown in figure 1, the invention provides a kind of method that enzyme process prepares organic rapeseed peptides, including:
Step one, rapeseed protein preparation: by Semen Allii Tuberosi peel after adopt normal hexane soak, obtain defat rapeseed meal after desolvation, its resid amount is 0.7～0.9%, and defat rapeseed meal was pulverized 40～80 mesh sieves, obtains rapeseed meal powder raw material；Being mixed by solid-liquid ratio 10～15:1 by the acetone that rapeseed meal powder raw material and concentration are 70%～80%, and carry out ultrasound-assisted extraction 15～25 minutes, extraction temperature is 45～50 DEG C, and ultrasound intensity is 1.2～1.4W/cm3, end to be extracted, dry and be extracted rapeseed meal powder；Mixing extracting rapeseed meal powder by solid-liquid ratio 8～12:1 with the ethanol that concentration is 80%～90%, and carry out ultrasound-assisted extraction 20～30 minutes, extraction temperature is 60～70 DEG C, and ultrasound intensity is 1.4～1.6W/cm3, end to be extracted, dry and obtain virus-free rapeseed meal powder；By the mixing of solid-liquid ratio 1:12～15, virus-free rapeseed meal powder being made rapeseed meal powder serosity with distilled water, regulates rapeseed meal powder serosity pH to 11～12, and carry out ultrasonic wave added alkali-soluble process 1～2 hour, alkali-soluble temperature is 45～55 DEG C, and ultrasound intensity is 1.0～1.2W/cm3Treat that alkali-soluble process terminates, it is centrifuged and obtains the supernatant, adjustment supernatant pH to 4.5 carries out the heavy process of acid 1.0～1.5 hours, treats that the heavy process of acid terminates, is centrifuged and obtains precipitation of sinking, lower sediment is washed to neutrality, and carry out lyophilization process, and namely obtaining rapeseed protein, the water absorption of described rapeseed protein is 1.03～4.21g/g, oil absorption be 0.52～2.37g/g, emulsibility is 32.4～59.4%；
Step 2, humid heat treatment: rapeseed protein and distilled water being pressed the mixing of solid-liquid ratio 1:8～12, and carries out ultrasonic wave added humid heat treatment 20～30 minutes, humid heat treatment temperature is 80～90 DEG C, and ultrasound intensity is 1.0～1.2W/cm3, obtain damp and hot rapeseed protein serosity；
Step 3, phosphatizing treatment: in damp and hot rapeseed protein serosity, add sodium tripolyphosphate carry out ultrasonic wave added phosphatizing treatment 1～1.5 hour, the addition of sodium tripolyphosphate is 1.5～2.5 times of damp and hot rapeseed protein serosity consumption, phosphatizing treatment temperature is 40～50 DEG C, and ultrasound intensity is 1.2～1.4W/cm3, treat that phosphatizing treatment terminates, obtain phosphorylation rapeseed protein serosity through dialysis；
Step 4, primary enzymolysis: regulate the pH to 8～10 of phosphorylation rapeseed protein serosity, and add alkaline protease and carry out ultrasonic wave added enzymolysis, the addition of alkaline protease is the 4.5～5.5% of rapeseed protein quality, enzyme digestion reaction temperature is 50～60 DEG C, the enzyme digestion reaction time is 1～3 hour, and ultrasound intensity is 1.4～1.6W/cm3, treat that primary enzymolysis reaction terminates, obtain primary enzymolysis liquid；
Step 5, secondary enzymolysis: regulate the pH to 5～7 of primary enzymolysis liquid, and add compound protease and carry out ultrasonic wave added enzymolysis, the addition of compound protease is the 4.5～5.5% of rapeseed protein quality, enzyme digestion reaction temperature is 45～55 DEG C, the enzyme digestion reaction time is 2～4 hours, and ultrasound intensity is 1.2～1.4W/cm3, treat that secondary enzymolysis reaction terminates, obtain secondary enzymolysis liquid；
Step 6, microwave deactivating enzyme: secondary enzymolysis liquid is carried out microwave deactivating enzyme process, microwave power is 500～800W, and microwave deactivating enzyme temperature is 80～90 DEG C, and the microwave deactivating enzyme time is 2～3 minutes, treats that microwave deactivating enzyme terminates, and obtains enzyme denaturing enzymolysis solution；Enzyme denaturing enzymolysis solution is centrifuged, take supernatant and obtain rapeseed peptides clear liquid, again rapeseed peptides clear liquid is carried out lyophilization process, the organic rapeseed peptides got product, the water absorption of described organic rapeseed peptides is 0.74～1.46g/g, oil absorption be 1.13～1.78g/g, foaming characteristic is 38.4～50.6%.
Enzyme process provided by the invention prepares the method for organic rapeseed peptides, by rapeseed meal powder raw material successively adopts acetone and ethanol carry out ultrasound on extracting, can effectively reduce the content of the noxious substances such as thioglycoside, phytic acid, polyphenol in rapeseed meal powder raw material；By rapeseed protein being sequentially carried out damp and hot and phosphatizing treatment, the efficiency of follow-up primary enzymolysis and secondary enzymolysis can be effectively improved；By adopting alkaline protease and compound protease that rapeseed protein is carried out ultrasonic wave added enzymolysis successively, the percent hydrolysis of rapeseed protein can be effectively improved；By adopting microwave deactivating enzyme, rapeseed peptides can be prevented effectively from and at high temperature inactivate；This enzyme process prepares the method for organic rapeseed peptides, process safety, prepare convenient.
Embodiment 1:
Adopting normal hexane to soak after being peeled by Semen Allii Tuberosi, obtain defat rapeseed meal after desolvation, its resid amount is 0.9%, and defat rapeseed meal was pulverized 60 mesh sieves, obtains rapeseed meal powder raw material；Being mixed by solid-liquid ratio 15:1 by the acetone that rapeseed meal powder raw material and concentration are 70%, and carry out ultrasound-assisted extraction 15 minutes, extraction temperature is 50 DEG C, and ultrasound intensity is 1.3W/cm3, end to be extracted, dry and be extracted rapeseed meal powder；Mixing extracting rapeseed meal powder by solid-liquid ratio 12:1 with the ethanol that concentration is 85%, and carry out ultrasound-assisted extraction 20 minutes, extraction temperature is 65 DEG C, and ultrasound intensity is 1.6W/cm3, end to be extracted, dry and obtain virus-free rapeseed meal powder；By solid-liquid ratio 1:12 mixing, virus-free rapeseed meal powder being made rapeseed meal powder serosity with distilled water, regulates rapeseed meal powder serosity pH to 12, and carry out ultrasonic wave added alkali-soluble process 1.5 hours, alkali-soluble temperature is 50 DEG C, and ultrasound intensity is 1.0W/cm3Treat that alkali-soluble process terminates, it is centrifuged and obtains the supernatant, regulating supernatant pH to 4.5 and carry out the heavy process of acid 1.5 hours, treat that the heavy process of acid terminates, centrifugal obtaining is precipitated, lower sediment is washed to neutrality, and carry out lyophilization process, and namely obtaining rapeseed protein, the water absorption of described rapeseed protein is 1.03g/g, oil absorption be 2.37g/g, emulsibility is 45.9%；Rapeseed protein and distilled water being pressed solid-liquid ratio 1:10 mixing, and carries out ultrasonic wave added humid heat treatment 20 minutes, humid heat treatment temperature is 90 DEG C, and ultrasound intensity is 1.1W/cm3, obtain damp and hot rapeseed protein serosity；Adding sodium tripolyphosphate in damp and hot rapeseed protein serosity and carry out ultrasonic wave added phosphatizing treatment 1 hour, the addition of sodium tripolyphosphate is 2 times of damp and hot rapeseed protein serosity consumption, and phosphatizing treatment temperature is 40 DEG C, and ultrasound intensity is 1.4W/cm3, treat that phosphatizing treatment terminates, obtain phosphorylation rapeseed protein serosity through dialysis；Regulating the pH to 9 of phosphorylation rapeseed protein serosity, and add alkaline protease and carry out ultrasonic wave added enzymolysis, the addition of alkaline protease is the 4.5% of rapeseed protein quality, and enzyme digestion reaction temperature is 60 DEG C, and the enzyme digestion reaction time is 2 hours, and ultrasound intensity is 1.5W/cm3, treat that primary enzymolysis reaction terminates, obtain primary enzymolysis liquid；Regulating the pH to 5 of primary enzymolysis liquid, and add compound protease and carry out ultrasonic wave added enzymolysis, the addition of compound protease is the 4.5% of rapeseed protein quality, and enzyme digestion reaction temperature is 50 DEG C, and the enzyme digestion reaction time is 2 hours, and ultrasound intensity is 1.4W/cm3, treat that secondary enzymolysis reaction terminates, obtain secondary enzymolysis liquid；Secondary enzymolysis liquid is carried out microwave deactivating enzyme process, and microwave power is 650W, and microwave deactivating enzyme temperature is 80 DEG C, and the microwave deactivating enzyme time is 2 minutes, treats that microwave deactivating enzyme terminates, and obtains enzyme denaturing enzymolysis solution；Enzyme denaturing enzymolysis solution is centrifuged, take supernatant and obtain rapeseed peptides clear liquid, again rapeseed peptides clear liquid being carried out lyophilization process, the organic rapeseed peptides got product, the water absorption of described organic rapeseed peptides is 0.74g/g, oil absorption be 1.78g/g, foaming characteristic is 44.5%.
Embodiment 2:
Adopting normal hexane to soak after being peeled by Semen Allii Tuberosi, obtain defat rapeseed meal after desolvation, its resid amount is 0.7%, and defat rapeseed meal was pulverized 80 mesh sieves, obtains rapeseed meal powder raw material；Being mixed by solid-liquid ratio 12:1 by the acetone that rapeseed meal powder raw material and concentration are 80%, and carry out ultrasound-assisted extraction 20 minutes, extraction temperature is 45 DEG C, and ultrasound intensity is 1.4W/cm3, end to be extracted, dry and be extracted rapeseed meal powder；Mixing extracting rapeseed meal powder by solid-liquid ratio 10:1 with the ethanol that concentration is 80%, and carry out ultrasound-assisted extraction 30 minutes, extraction temperature is 60 DEG C, and ultrasound intensity is 1.5W/cm3, end to be extracted, dry and obtain virus-free rapeseed meal powder；By solid-liquid ratio 1:15 mixing, virus-free rapeseed meal powder being made rapeseed meal powder serosity with distilled water, regulates rapeseed meal powder serosity pH to 11, and carry out ultrasonic wave added alkali-soluble process 2 hours, alkali-soluble temperature is 45 DEG C, and ultrasound intensity is 1.2W/cm3Treat that alkali-soluble process terminates, it is centrifuged and obtains the supernatant, regulating supernatant pH to 4.5 and carry out the heavy process of acid 1.0 hours, treat that the heavy process of acid terminates, centrifugal obtaining is precipitated, lower sediment is washed to neutrality, and carry out lyophilization process, and namely obtaining rapeseed protein, the water absorption of described rapeseed protein is 4.21g/g, oil absorption be 1.45g/g, emulsibility is 32.4%；Rapeseed protein and distilled water being pressed solid-liquid ratio 1:12 mixing, and carries out ultrasonic wave added humid heat treatment 25 minutes, humid heat treatment temperature is 80 DEG C, and ultrasound intensity is 1.2W/cm3, obtain damp and hot rapeseed protein serosity；Adding sodium tripolyphosphate in damp and hot rapeseed protein serosity and carry out ultrasonic wave added phosphatizing treatment 1.5 hours, the addition of sodium tripolyphosphate is 1.5 times of damp and hot rapeseed protein serosity consumption, and phosphatizing treatment temperature is 45 DEG C, and ultrasound intensity is 1.2W/cm3, treat that phosphatizing treatment terminates, obtain phosphorylation rapeseed protein serosity through dialysis；Regulating the pH to 10 of phosphorylation rapeseed protein serosity, and add alkaline protease and carry out ultrasonic wave added enzymolysis, the addition of alkaline protease is the 5.0% of rapeseed protein quality, and enzyme digestion reaction temperature is 55 DEG C, and the enzyme digestion reaction time is 3 hours, and ultrasound intensity is 1.4W/cm3, treat that primary enzymolysis reaction terminates, obtain primary enzymolysis liquid；Regulating the pH to 6 of primary enzymolysis liquid, and add compound protease and carry out ultrasonic wave added enzymolysis, the addition of compound protease is the 5.0% of rapeseed protein quality, and enzyme digestion reaction temperature is 55 DEG C, and the enzyme digestion reaction time is 3 hours, and ultrasound intensity is 1.2W/cm3, treat that secondary enzymolysis reaction terminates, obtain secondary enzymolysis liquid；Secondary enzymolysis liquid is carried out microwave deactivating enzyme process, and microwave power is 500W, and microwave deactivating enzyme temperature is 90 DEG C, and the microwave deactivating enzyme time is 3 minutes, treats that microwave deactivating enzyme terminates, and obtains enzyme denaturing enzymolysis solution；Enzyme denaturing enzymolysis solution is centrifuged, take supernatant and obtain rapeseed peptides clear liquid, again rapeseed peptides clear liquid being carried out lyophilization process, the organic rapeseed peptides got product, the water absorption of described organic rapeseed peptides is 1.46g/g, oil absorption be 1.46g/g, foaming characteristic is 38.4%.
Embodiment 3:
Adopting normal hexane to soak after being peeled by Semen Allii Tuberosi, obtain defat rapeseed meal after desolvation, its resid amount is 0.8%, and defat rapeseed meal was pulverized 40 mesh sieves, obtains rapeseed meal powder raw material；Being mixed by solid-liquid ratio 10:1 by the acetone that rapeseed meal powder raw material and concentration are 75%, and carry out ultrasound-assisted extraction 25 minutes, extraction temperature is 48 DEG C, and ultrasound intensity is 1.2W/cm3, end to be extracted, dry and be extracted rapeseed meal powder；Mixing extracting rapeseed meal powder by solid-liquid ratio 8:1 with the ethanol that concentration is 90%, and carry out ultrasound-assisted extraction 25 minutes, extraction temperature is 70 DEG C, and ultrasound intensity is 1.4W/cm3, end to be extracted, dry and obtain virus-free rapeseed meal powder；By solid-liquid ratio 1:13 mixing, virus-free rapeseed meal powder being made rapeseed meal powder serosity with distilled water, regulates rapeseed meal powder serosity pH to 11, and carry out ultrasonic wave added alkali-soluble process 1 hour, alkali-soluble temperature is 55 DEG C, and ultrasound intensity is 1.1W/cm3Treat that alkali-soluble process terminates, it is centrifuged and obtains the supernatant, regulating supernatant pH to 4.5 and carry out the heavy process of acid 1.2 hours, treat that the heavy process of acid terminates, centrifugal obtaining is precipitated, lower sediment is washed to neutrality, and carry out lyophilization process, and namely obtaining rapeseed protein, the water absorption of described rapeseed protein is 2.62g/g, oil absorption be 0.52g/g, emulsibility is 59.4%；Rapeseed protein and distilled water being pressed solid-liquid ratio 1:8 mixing, and carries out ultrasonic wave added humid heat treatment 30 minutes, humid heat treatment temperature is 85 DEG C, and ultrasound intensity is 1.0W/cm3, obtain damp and hot rapeseed protein serosity；Adding sodium tripolyphosphate in damp and hot rapeseed protein serosity and carry out ultrasonic wave added phosphatizing treatment 1.2 hours, the addition of sodium tripolyphosphate is 2.5 times of damp and hot rapeseed protein serosity consumption, and phosphatizing treatment temperature is 50 DEG C, and ultrasound intensity is 1.3W/cm3, treat that phosphatizing treatment terminates, obtain phosphorylation rapeseed protein serosity through dialysis；Regulating the pH to 8 of phosphorylation rapeseed protein serosity, and add alkaline protease and carry out ultrasonic wave added enzymolysis, the addition of alkaline protease is the 5.5% of rapeseed protein quality, and enzyme digestion reaction temperature is 50 DEG C, and the enzyme digestion reaction time is 1 hour, and ultrasound intensity is 1.6W/cm3, treat that primary enzymolysis reaction terminates, obtain primary enzymolysis liquid；Regulating the pH to 7 of primary enzymolysis liquid, and add compound protease and carry out ultrasonic wave added enzymolysis, the addition of compound protease is the 5.5% of rapeseed protein quality, and enzyme digestion reaction temperature is 45 DEG C, and the enzyme digestion reaction time is 4 hours, and ultrasound intensity is 1.3W/cm3, treat that secondary enzymolysis reaction terminates, obtain secondary enzymolysis liquid；Secondary enzymolysis liquid is carried out microwave deactivating enzyme process, and microwave power is 800W, and microwave deactivating enzyme temperature is 85 DEG C, and the microwave deactivating enzyme time is 2.5 minutes, treats that microwave deactivating enzyme terminates, and obtains enzyme denaturing enzymolysis solution；Enzyme denaturing enzymolysis solution is centrifuged, take supernatant and obtain rapeseed peptides clear liquid, again rapeseed peptides clear liquid being carried out lyophilization process, the organic rapeseed peptides got product, the water absorption of described organic rapeseed peptides is 1.10g/g, oil absorption be 1.13g/g, foaming characteristic is 50.6%.
Although embodiment of the present invention are disclosed as above, but listed utilization that it is not limited in description and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, it is easily achieved other amendment, therefore, under the general concept limited without departing substantially from claim and equivalency range, the present invention is not limited to specific details and shown here as the legend with description.
Claims (9)
1. the method that an enzyme process prepares organic rapeseed peptides, it is characterised in that including:
Step one, rapeseed protein preparation；
Step 2, humid heat treatment: by rapeseed protein with distilled water by the mixing of certain solid-liquid ratio, and carry out ultrasonic wave added humid heat treatment, obtain damp and hot rapeseed protein serosity；
Step 3, phosphatizing treatment: in damp and hot rapeseed protein serosity, add sodium tripolyphosphate carry out ultrasonic wave added phosphatizing treatment, treat that phosphatizing treatment terminates, obtain phosphorylation rapeseed protein serosity through dialysis；
Step 4, primary enzymolysis: regulate the pH of phosphorylation rapeseed protein serosity and temperature, and add alkaline protease and carry out ultrasonic wave added enzymolysis, obtain primary enzymolysis liquid；
Step 5, secondary enzymolysis: regulate the pH of primary enzymolysis liquid and temperature, and add compound protease and carry out ultrasonic wave added enzymolysis, obtain secondary enzymolysis liquid；
Step 6, microwave deactivating enzyme: secondary enzymolysis liquid is carried out microwave deactivating enzyme process, obtain enzyme denaturing enzymolysis solution, obtains rapeseed peptides clear liquid by centrifugal for enzyme denaturing enzymolysis solution, then rapeseed peptides clear liquid is carried out lyophilization process, the organic rapeseed peptides got product.
2. the method that enzyme process as claimed in claim 1 prepares organic rapeseed peptides, it is characterized in that, rapeseed meal powder raw material is carried out detoxification treatment, obtain virus-free rapeseed meal powder, virus-free rapeseed meal powder is made rapeseed meal powder serosity with distilled water by the mixing of solid-liquid ratio 1:12～15, regulates rapeseed meal powder serosity pH to 11～12, and carry out ultrasonic wave added alkali-soluble process 1～2 hour, alkali-soluble temperature is 45～55 DEG C, and ultrasound intensity is 1.0～1.2W/cm3Treat that alkali-soluble process terminates, it is centrifuged and obtains the supernatant, adjustment supernatant pH to 4.5 carries out the heavy process of acid 1.0～1.5 hours, treats that the heavy process of acid terminates, is centrifuged and obtains precipitation of sinking, lower sediment is washed to neutrality, and carry out lyophilization process, and namely obtaining rapeseed protein, the water absorption of described rapeseed protein is 1.03～4.21g/g, oil absorption be 0.52～2.37g/g, emulsibility is 32.4～59.4%.
3. the method that enzyme process as claimed in claim 2 prepares organic rapeseed peptides, it is characterized in that, the preparation process of described rapeseed meal powder raw material is as follows: adopt normal hexane to soak after being peeled by Semen Allii Tuberosi, defat rapeseed meal is obtained after desolvation, its resid amount is 0.7～0.9%, defat rapeseed meal is pulverized 40～80 mesh sieves, obtains rapeseed meal powder raw material.
4. the method that enzyme process as claimed in claim 3 prepares organic rapeseed peptides, it is characterized in that, the process of described detoxification treatment is as follows: mixed by solid-liquid ratio 10～15:1 by the acetone that rapeseed meal powder raw material and concentration are 70%～80%, and carry out ultrasound-assisted extraction 15～25 minutes, extraction temperature is 45～50 DEG C, and ultrasound intensity is 1.2～1.4W/cm3, end to be extracted, dry and be extracted rapeseed meal powder；Mixing extracting rapeseed meal powder by solid-liquid ratio 8～12:1 with the ethanol that concentration is 80%～90%, and carry out ultrasound-assisted extraction 20～30 minutes, extraction temperature is 60～70 DEG C, and ultrasound intensity is 1.4～1.6W/cm3, end to be extracted, dry and obtain virus-free rapeseed meal powder.
5. the method that enzyme process as claimed in claim 1 prepares organic rapeseed peptides, it is characterized in that, in described step 2, the process of humid heat treatment is as follows: rapeseed protein and distilled water are pressed the mixing of solid-liquid ratio 1:8～12, and carry out ultrasonic wave added humid heat treatment 20～30 minutes, humid heat treatment temperature is 80～90 DEG C, and ultrasound intensity is 1.0～1.2W/cm3, obtain damp and hot rapeseed protein serosity.
6. the method that enzyme process as claimed in claim 1 prepares organic rapeseed peptides, it is characterized in that, in described step 3, the process of phosphatizing treatment is as follows: adds sodium tripolyphosphate in damp and hot rapeseed protein serosity and carries out ultrasonic wave added phosphatizing treatment 1～1.5 hour, the addition of sodium tripolyphosphate is 1.5～2.5 times of damp and hot rapeseed protein serosity consumption, phosphatizing treatment temperature is 40～50 DEG C, and ultrasound intensity is 1.2～1.4W/cm3, treat that phosphatizing treatment terminates, obtain phosphorylation rapeseed protein serosity through dialysis.
7. the method that enzyme process as claimed in claim 1 prepares organic rapeseed peptides, it is characterized in that, in described step 4, the process of primary enzymolysis is as follows: regulate the pH to 8～10 of phosphorylation rapeseed protein serosity, and add alkaline protease and carry out ultrasonic wave added enzymolysis, the addition of alkaline protease is the 4.5～5.5% of rapeseed protein quality, enzyme digestion reaction temperature is 50～60 DEG C, and the enzyme digestion reaction time is 1～3 hour, and ultrasound intensity is 1.4～1.6W/cm3, treat that primary enzymolysis reaction terminates, obtain primary enzymolysis liquid.
8. the method that enzyme process as claimed in claim 1 prepares organic rapeseed peptides, it is characterized in that, in described step 5, the process of secondary enzymolysis is as follows: regulate the pH to 5～7 of primary enzymolysis liquid, and add compound protease and carry out ultrasonic wave added enzymolysis, the addition of compound protease is the 4.5～5.5% of rapeseed protein quality, enzyme digestion reaction temperature is 45～55 DEG C, and the enzyme digestion reaction time is 2～4 hours, and ultrasound intensity is 1.2～1.4W/cm3, treat that secondary enzymolysis reaction terminates, obtain secondary enzymolysis liquid.
9. the method that enzyme process as claimed in claim 1 prepares organic rapeseed peptides, it is characterized in that, in described step 6, the process of microwave deactivating enzyme is as follows: secondary enzymolysis liquid is carried out microwave deactivating enzyme process, microwave power is 500～800W, microwave deactivating enzyme temperature is 80～90 DEG C, the microwave deactivating enzyme time is 2～3 minutes, treats that microwave deactivating enzyme terminates, and obtains enzyme denaturing enzymolysis solution；Enzyme denaturing enzymolysis solution is centrifuged, take supernatant and obtain rapeseed peptides clear liquid, again rapeseed peptides clear liquid is carried out lyophilization process, the organic rapeseed peptides got product, the water absorption of described organic rapeseed peptides is 0.74～1.46g/g, oil absorption be 1.13～1.78g/g, foaming characteristic is 38.4～50.6%.
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| CN108866138A (en) * | 2018-08-29 | 2018-11-23 | 宝得瑞（湖北）健康产业有限公司 | A kind of preparation method of pumpkin polypeptide |
| CN115644362A (en) * | 2022-11-11 | 2023-01-31 | 湖南省农产品加工研究所 | Low-salt fermented bean curd and preparation method thereof |
| CN116268344A (en) * | 2023-03-06 | 2023-06-23 | 鼎新思远重庆粮油食品工业有限公司 | Process method for producing edible rapeseed powder and automatic extractor |
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