CN105779542B - A kind of method that enzyme process prepares organic rapeseed peptides - Google Patents
A kind of method that enzyme process prepares organic rapeseed peptides Download PDFInfo
- Publication number
- CN105779542B CN105779542B CN201610225648.8A CN201610225648A CN105779542B CN 105779542 B CN105779542 B CN 105779542B CN 201610225648 A CN201610225648 A CN 201610225648A CN 105779542 B CN105779542 B CN 105779542B
- Authority
- CN
- China
- Prior art keywords
- rapeseed
- enzyme
- peptides
- carries out
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of methods that enzyme process prepares organic rapeseed peptides, comprising: the preparation of rapeseed protein；Rapeseed protein and distilled water are mixed by certain solid-liquid ratio, and carry out ultrasonic wave added humid heat treatment, obtains damp and hot rapeseed protein slurries；Sodium tripolyphosphate is added into damp and hot rapeseed protein slurries and carries out ultrasonic wave added phosphatizing treatment, obtains phosphorylation rapeseed protein slurries；The pH and temperature of phosphorylation rapeseed protein slurries are adjusted, and alkali protease is added and carries out ultrasonic wave added enzymatic hydrolysis, obtains primary enzymolysis liquid；The pH and temperature of primary enzymolysis liquid are adjusted, and compound fertilizer production is added and carries out ultrasonic wave added enzymatic hydrolysis, obtains secondary enzymatic solution；Microwave deactivating enzyme processing is carried out to secondary enzymatic solution, enzyme deactivation enzymolysis liquid is obtained, enzyme deactivation enzymolysis liquid is centrifuged to obtain rapeseed peptides clear liquid, then carry out freeze-drying process to get organic rapeseed peptides of finished product.The method that the enzyme process prepares organic rapeseed peptides, process safety, prepare it is convenient.
Description
Technical field
The present invention relates to rapeseed peptides technical fields, and in particular to a kind of method that enzyme process prepares organic rapeseed peptides.
Background technique
Rapeseed is a kind of important oil crops, and China is the rapeseed product big country for occupying the first in the world, rapeseed
Resource very abundant, 100,000,000 mu of cultivated area or more of rapeseed, yield is up to more than 1,300 ten thousand tons, occupies first of the world, annual to process
The rapeseed dregs generated in the process is up to more than 600 ten thousand tons.Rich in protein in rapeseed, up to 25~27%, after leaching oil removing
Rapeseed dregs in protein content be up to 35~45%.In addition, rapeseed protein is a kind of high-quality protein, amino acid forms and contains
Amount meets FAO/WHO recommendation pattern value, there's almost no limiting amino acid, nutrition can mutually be equal to casein and animal protein
Beauty, compared with the other plants albumen such as soybean, peanut, cottonseed, biological value and nutritive value are higher, are a kind of with exploitation
The quality plant protein of potentiality.Rapeseed protein is degraded into peptides, the utilization rate and economic value of rapeseed protein can be improved, this
A little peptides have the advantage that human consumption and absorption are more advantageous to than albumen, and have certain bioactivity, such as adjust and exempt from
Epidemic disease, blood pressure lowering and anti-oxidant etc..Therefore, it is necessary to study a kind of methods for preparing organic rapeseed peptides.
Summary of the invention
Shortcoming present in view of the above technology the present invention provides a kind of process safety, prepares convenient and fast enzyme process
The method for preparing organic rapeseed peptides.
The technical solution adopted by the present invention to solve the technical problems is: a kind of enzyme process prepares the side of organic rapeseed peptides
Method, comprising: Step 1: the preparation of rapeseed protein；Step 2: humid heat treatment: rapeseed protein and distilled water are pressed certain solid-liquid ratio
It mixes, and carries out ultrasonic wave added humid heat treatment, obtain damp and hot rapeseed protein slurries；Step 3: phosphatizing treatment: to damp and hot vegetable seed
Sodium tripolyphosphate is added in protein slurry and carries out ultrasonic wave added phosphatizing treatment, to the end of phosphatizing treatment, obtains phosphorus through dialysis
It is acidified rapeseed protein slurries；Step 4: primary enzymolysis: adjusting the pH and temperature of phosphorylation rapeseed protein slurries, and alkalinity is added
Protease carries out ultrasonic wave added enzymatic hydrolysis, obtains primary enzymolysis liquid；Step 5: secondary enzymolysis: adjusting the pH and temperature of primary enzymolysis liquid
Degree, and compound fertilizer production is added and carries out ultrasonic wave added enzymatic hydrolysis, obtain secondary enzymatic solution；Step 6: microwave deactivating enzyme: to secondary
Enzymolysis liquid carries out microwave deactivating enzyme processing, obtains enzyme deactivation enzymolysis liquid, enzyme deactivation enzymolysis liquid is centrifuged to obtain rapeseed peptides clear liquid, then to dish
Seed polypeptide clear liquid carries out freeze-drying process to get organic rapeseed peptides of finished product.
Preferably, rapeseed dregs powder raw material is subjected to detoxification treatment, obtains virus-free rapeseed meal powder, by virus-free rapeseed meal powder and steamed
Rapeseed dregs powder slurries are made by the mixing of solid-liquid ratio 1:12~15 in distilled water, adjust rapeseed dregs powder slurries pH to 11~12, and surpassed
Sound assists alkali soluble to handle 1~2 hour, and alkali soluble temperature is 45~55 DEG C, and ultrasound intensity is 1.0~1.2W/cm3, to alkali soluble processing
Terminate, centrifugation obtains supernatant liquor, adjusts supernatant liquor pH to 4.5 and carries out the heavy processing of acid 1.0~1.5 hours, to the heavy processing of acid
Terminating, centrifugation obtains precipitating, lower sediment is washed to neutrality, and carry out freeze-drying process to get to rapeseed protein,
The water imbibition of the rapeseed protein is 1.03~4.21g/g, oil absorption is 0.52~2.37g/g, emulsibility be 32.4~
59.4%。
Preferably, the preparation process of the rapeseed dregs powder raw material is as follows: being impregnated after vegetable seed is peeled using n-hexane, removing
Degreasing rapeseed dregs is obtained after solvent, resid amount is 0.7~0.9%, and degreasing rapeseed dregs crushed 40~80 meshes to get dish
Seed dregs of rice powder raw material.
Preferably, the process of the detoxification treatment is as follows: the acetone that rapeseed dregs powder raw material is 70%~80% with concentration is pressed
10~15:1 of solid-liquid ratio is mixed, and carries out ultrasound-assisted extraction 15~25 minutes, and extraction temperature is 45~50 DEG C, ultrasound intensity
For 1.2~1.4W/cm3, end to be extracted is dried to obtain extraction rapeseed dregs powder；Again will extraction rapeseed dregs powder and concentration be 80%~
90% ethyl alcohol is mixed by 8~12:1 of solid-liquid ratio, and carries out ultrasound-assisted extraction 20~30 minutes, and extraction temperature is 60~70
DEG C, ultrasound intensity is 1.4~1.6W/cm3, end to be extracted is dried to obtain virus-free rapeseed meal powder.
Preferably, the process of humid heat treatment is as follows in the step 2: by rapeseed protein and distilled water by solid-liquid ratio 1:8~
12 mix, and carry out ultrasonic wave added humid heat treatment 20~30 minutes, and humid heat treatment temperature is 80~90 DEG C, ultrasound intensity 1.0
~1.2W/cm3, obtain damp and hot rapeseed protein slurries.
Preferably, the process of phosphatizing treatment is as follows in the step 3: trimerization is added into damp and hot rapeseed protein slurries
Sodium phosphate carries out ultrasonic wave added phosphatizing treatment 1~1.5 hour, and the additional amount of sodium tripolyphosphate is damp and hot rapeseed protein slurries use
1.5~2.5 times of amount, phosphatizing treatment temperature are 40~50 DEG C, and ultrasound intensity is 1.2~1.4W/cm3, to phosphatizing treatment
Terminate, obtains phosphorylation rapeseed protein slurries through dialysis.
Preferably, the process of primary enzymolysis is as follows in the step 4: the pH to 8 of adjusting phosphorylation rapeseed protein slurries~
10, and alkali protease is added and carries out ultrasonic wave added enzymatic hydrolysis, the additional amount of alkali protease be rapeseed protein quality 4.5~
5.5%, enzymolysis temperature is 50~60 DEG C, and the enzyme digestion reaction time is 1~3 hour, and ultrasound intensity is 1.4~1.6W/cm3, to
Primary enzymolysis reaction terminates, and obtains primary enzymolysis liquid.
Preferably, the process of secondary enzymolysis is as follows in the step 5: adjusting the pH to 5~7 of primary enzymolysis liquid, and is added
Compound fertilizer production carries out ultrasonic wave added enzymatic hydrolysis, the additional amount of compound fertilizer production be rapeseed protein quality 4.5~
5.5%, enzymolysis temperature is 45~55 DEG C, and the enzyme digestion reaction time is 2~4 hours, and ultrasound intensity is 1.2~1.4W/cm3, to
Secondary enzymolysis reaction terminates, and obtains secondary enzymatic solution.
Preferably, the process of microwave deactivating enzyme is as follows in the step 6: microwave deactivating enzyme processing is carried out to secondary enzymatic solution, it is micro-
Wave power is 500~800W, and microwave deactivating enzyme temperature is 80~90 DEG C, and the microwave deactivating enzyme time is 2~3 minutes, to microwave deactivating enzyme knot
Beam obtains enzyme deactivation enzymolysis liquid；Enzyme deactivation enzymolysis liquid is centrifuged, takes supernatant to obtain rapeseed peptides clear liquid, then to rapeseed peptides clear liquid
Freeze-drying process being carried out to get organic rapeseed peptides of finished product, the water imbibitions of organic rapeseed peptides is 0.74~
1.46g/g, oil absorption are 1.13~1.78g/g, foaming characteristic is 38.4~50.6%.
Compared with prior art, the present invention the beneficial effect is that: enzyme process provided by the invention prepares organic rapeseed peptides
Method can effectively reduce rapeseed dregs powder by successively using acetone and ethyl alcohol to carry out ultrasound on extracting rapeseed dregs powder raw material
The content of the noxious materials such as glucosinolate, phytic acid, polyphenol in raw material；By successively carrying out damp and hot and phosphoric acid to rapeseed protein
Change processing, can effectively improve the efficiency of subsequent primary enzymolysis and secondary enzymolysis；By successively using alkali protease and compound wind
Taste protease carries out ultrasonic wave added enzymatic hydrolysis to rapeseed protein, can effectively improve the percent hydrolysis of rapeseed protein；It goes out by using microwave
Enzyme can effectively avoid rapeseed peptides and inactivate at high temperature；The method that the enzyme process prepares organic rapeseed peptides, process safety, preparation
It is convenient.
Detailed description of the invention
Fig. 1 is the process flow chart for the method that enzyme process of the present invention prepares organic rapeseed peptides.
Specific embodiment
Present invention will be described in further detail below with reference to the accompanying drawings, to enable those skilled in the art referring to specification text
Word can be implemented accordingly.
As shown in Figure 1, the present invention provides a kind of methods that enzyme process prepares organic rapeseed peptides, comprising:
Step 1: the preparation of rapeseed protein: being impregnated after vegetable seed is peeled using n-hexane, obtain degreasing dish after removing solvent
The seed dregs of rice, resid amount are 0.7~0.9%, and degreasing rapeseed dregs crushed 40~80 meshes to get rapeseed dregs powder raw material；By vegetable seed
The acetone that dregs of rice powder raw material and concentration are 70%~80% is mixed by 10~15:1 of solid-liquid ratio, and carries out ultrasound-assisted extraction 15~25
Minute, extraction temperature is 45~50 DEG C, and ultrasound intensity is 1.2~1.4W/cm3, end to be extracted is dried to obtain extraction rapeseed dregs
Powder；The ethyl alcohol that rapeseed dregs powder and concentration are 80%~90% will be extracted again to mix by 8~12:1 of solid-liquid ratio, and carries out ultrasonic wave auxiliary
Extraction 20~30 minutes, extraction temperature are 60~70 DEG C, and ultrasound intensity is 1.4~1.6W/cm3, end to be extracted is dried to obtain
Virus-free rapeseed meal powder；Rapeseed dregs powder slurries are made by the mixing of solid-liquid ratio 1:12~15 in virus-free rapeseed meal powder and distilled water, are adjusted
Rapeseed dregs powder slurries pH to 11~12, and carry out ultrasonic wave added alkali soluble and handle 1~2 hour, alkali soluble temperature is 45~55 DEG C, ultrasound
Intensity is 1.0~1.2W/cm3, to alkali soluble, processing terminate, and centrifugation obtains supernatant liquor, adjusts supernatant liquor pH to 4.5 and carries out acid
Heavy processing 1.0~1.5 hours, to acid it is heavy processing terminate, centrifugation obtains precipitating, and lower sediment is washed to neutrality, is gone forward side by side
Row freeze-drying process to get to rapeseed protein, the rapeseed protein water imbibition be 1.03~4.21g/g, oil absorption is
0.52~2.37g/g, emulsibility are 32.4~59.4%；
Step 2: humid heat treatment: rapeseed protein and distilled water being mixed by solid-liquid ratio 1:8~12, and carry out ultrasonic wave added
Humid heat treatment 20~30 minutes, humid heat treatment temperature was 80~90 DEG C, and ultrasound intensity is 1.0~1.2W/cm3, obtain damp and hot dish
Seed protein slurry；
Step 3: phosphatizing treatment: sodium tripolyphosphate is added into damp and hot rapeseed protein slurries and carries out ultrasonic wave added phosphoric acid
Change processing 1~1.5 hour, the additional amount of sodium tripolyphosphate is 1.5~2.5 times of damp and hot rapeseed protein slurries dosage, phosphorylation
Treatment temperature is 40~50 DEG C, and ultrasound intensity is 1.2~1.4W/cm3, to the end of phosphatizing treatment, phosphorylation is obtained through dialysis
Rapeseed protein slurries；
Step 4: primary enzymolysis: adjust phosphorylation rapeseed protein slurries pH to 8~10, and be added alkali protease into
Row ultrasonic wave added enzymatic hydrolysis, the additional amount of alkali protease are the 4.5~5.5% of rapeseed protein quality, enzymolysis temperature is 50~
60 DEG C, the enzyme digestion reaction time is 1~3 hour, and ultrasound intensity is 1.4~1.6W/cm3, to the end of primary enzymolysis reaction, obtain one
Secondary enzymolysis liquid；
Step 5: secondary enzymolysis: adjusting the pH to 5~7 of primary enzymolysis liquid, and compound fertilizer production is added and carries out ultrasound
Assistance enzymolysis, the additional amount of compound fertilizer production are the 4.5~5.5% of rapeseed protein quality, and enzymolysis temperature is 45~55
DEG C, the enzyme digestion reaction time is 2~4 hours, and ultrasound intensity is 1.2~1.4W/cm3, to the end of secondary enzymolysis reaction, obtain secondary
Enzymolysis liquid；
Step 6: microwave deactivating enzyme: carrying out microwave deactivating enzyme processing to secondary enzymatic solution, microwave power is 500~800W, microwave
Enzyme-removal temperature is 80~90 DEG C, and the microwave deactivating enzyme time is 2~3 minutes, to the end of microwave deactivating enzyme, obtains enzyme deactivation enzymolysis liquid；It will go out
The centrifugation of enzyme enzymolysis liquid, takes supernatant to obtain rapeseed peptides clear liquid, then to rapeseed peptides clear liquid carry out freeze-drying process to get at
Organic rapeseed peptides of product, the water imbibition of organic rapeseed peptides is 0.74~1.46g/g, oil absorption is 1.13~1.78g/
G, foaming characteristic is 38.4~50.6%.
The method that enzyme process provided by the invention prepares organic rapeseed peptides, by successively using acetone to rapeseed dregs powder raw material
Ultrasound on extracting is carried out with ethyl alcohol, can effectively reduce the Toxics such as glucosinolate, phytic acid, polyphenol in rapeseed dregs powder raw material
The content of matter；By successively carrying out damp and hot and phosphatizing treatment to rapeseed protein, subsequent primary enzymolysis and secondary can be effectively improved
The efficiency of enzymatic hydrolysis；By successively alkali protease and compound fertilizer production being used to carry out ultrasonic wave added enzymatic hydrolysis to rapeseed protein,
The percent hydrolysis of rapeseed protein can be effectively improved；By using microwave deactivating enzyme, it can effectively avoid rapeseed peptides and inactivate at high temperature；It should
The method that enzyme process prepares organic rapeseed peptides, process safety, prepare it is convenient.
Embodiment 1:
It is impregnated after vegetable seed is peeled using n-hexane, obtains degreasing rapeseed dregs after removing solvent, resid amount 0.9% will
Degreasing rapeseed dregs crushed 60 meshes to get rapeseed dregs powder raw material；The acetone that rapeseed dregs powder raw material and concentration are 70% is pressed into feed liquid
It is mixed than 15:1, and carries out ultrasound-assisted extraction 15 minutes, extraction temperature is 50 DEG C, ultrasound intensity 1.3W/cm3, wait extract
End is taken, extraction rapeseed dregs powder is dried to obtain；The ethyl alcohol that rapeseed dregs powder is 85% with concentration will be extracted again to mix by solid-liquid ratio 12:1
It is even, and carry out ultrasound-assisted extraction 20 minutes, extraction temperature is 65 DEG C, ultrasound intensity 1.6W/cm3, end to be extracted, do
It is dry to obtain virus-free rapeseed meal powder；Rapeseed dregs powder slurries are made by solid-liquid ratio 1:12 mixing in virus-free rapeseed meal powder and distilled water, are adjusted
Rapeseed dregs powder slurries pH to 12 is saved, and carries out ultrasonic wave added alkali soluble and handles 1.5 hours, alkali soluble temperature is 50 DEG C, and ultrasound intensity is
1.0W/cm3, to alkali soluble, processing terminate, and centrifugation obtains supernatant liquor, and it is small to adjust the heavy processing 1.5 of the progress acid of supernatant liquor pH to 4.5
When, to acid it is heavy processing terminate, centrifugation obtains precipitating, lower sediment is washed to neutrality, and carry out freeze-drying process, i.e.,
Obtain rapeseed protein, the water imbibition of the rapeseed protein is 1.03g/g, oil absorption 2.37g/g, emulsibility 45.9%；By dish
Seed albumen and distilled water are mixed by solid-liquid ratio 1:10, and carry out ultrasonic wave added humid heat treatment 20 minutes, and humid heat treatment temperature is 90
DEG C, ultrasound intensity 1.1W/cm3, obtain damp and hot rapeseed protein slurries；Sodium tripolyphosphate is added into damp and hot rapeseed protein slurries
It carries out ultrasonic wave added phosphatizing treatment 1 hour, the additional amount of sodium tripolyphosphate is 2 times of damp and hot rapeseed protein slurries dosage, phosphorus
Acidification temperature is 40 DEG C, ultrasound intensity 1.4W/cm3, to the end of phosphatizing treatment, phosphorylation vegetable seed egg is obtained through dialysis
White slurries；The pH to 9 of phosphorylation rapeseed protein slurries is adjusted, and alkali protease is added and carries out ultrasonic wave added enzymatic hydrolysis, alkaline egg
The additional amount of white enzyme is the 4.5% of rapeseed protein quality, and enzymolysis temperature is 60 DEG C, and the enzyme digestion reaction time is 2 hours, ultrasound
Intensity is 1.5W/cm3, to the end of primary enzymolysis reaction, obtain primary enzymolysis liquid；The pH to 5 of primary enzymolysis liquid is adjusted, and is added
Compound fertilizer production carries out ultrasonic wave added enzymatic hydrolysis, and the additional amount of compound fertilizer production is the 4.5% of rapeseed protein quality, enzyme
Solving reaction temperature is 50 DEG C, and the enzyme digestion reaction time is 2 hours, ultrasound intensity 1.4W/cm3, to the end of secondary enzymolysis reaction, obtain
To secondary enzymatic solution；Microwave deactivating enzyme processing, microwave power 650W are carried out to secondary enzymatic solution, microwave deactivating enzyme temperature is 80 DEG C,
The microwave deactivating enzyme time is 2 minutes, to the end of microwave deactivating enzyme, obtains enzyme deactivation enzymolysis liquid；Enzyme deactivation enzymolysis liquid is centrifuged, supernatant is taken to obtain
It is described to have to rapeseed peptides clear liquid, then to rapeseed peptides clear liquid progress freeze-drying process to get organic rapeseed peptides of finished product
The water imbibition of machine rapeseed peptides is 0.74g/g, oil absorption 1.78g/g, foaming characteristic 44.5%.
Embodiment 2:
It is impregnated after vegetable seed is peeled using n-hexane, obtains degreasing rapeseed dregs after removing solvent, resid amount 0.7% will
Degreasing rapeseed dregs crushed 80 meshes to get rapeseed dregs powder raw material；The acetone that rapeseed dregs powder raw material and concentration are 80% is pressed into feed liquid
It is mixed than 12:1, and carries out ultrasound-assisted extraction 20 minutes, extraction temperature is 45 DEG C, ultrasound intensity 1.4W/cm3, wait extract
End is taken, extraction rapeseed dregs powder is dried to obtain；The ethyl alcohol that rapeseed dregs powder is 80% with concentration will be extracted again to mix by solid-liquid ratio 10:1
It is even, and carry out ultrasound-assisted extraction 30 minutes, extraction temperature is 60 DEG C, ultrasound intensity 1.5W/cm3, end to be extracted, do
It is dry to obtain virus-free rapeseed meal powder；Rapeseed dregs powder slurries are made by solid-liquid ratio 1:15 mixing in virus-free rapeseed meal powder and distilled water, are adjusted
Rapeseed dregs powder slurries pH to 11 is saved, and carries out ultrasonic wave added alkali soluble and handles 2 hours, alkali soluble temperature is 45 DEG C, and ultrasound intensity is
1.2W/cm3, to alkali soluble, processing terminate, and centrifugation obtains supernatant liquor, and it is small to adjust the heavy processing 1.0 of the progress acid of supernatant liquor pH to 4.5
When, to acid it is heavy processing terminate, centrifugation obtains precipitating, lower sediment is washed to neutrality, and carry out freeze-drying process, i.e.,
Obtain rapeseed protein, the water imbibition of the rapeseed protein is 4.21g/g, oil absorption 1.45g/g, emulsibility 32.4%；It will
Rapeseed protein and distilled water are mixed by solid-liquid ratio 1:12, and carry out ultrasonic wave added humid heat treatment 25 minutes, and humid heat treatment temperature is
80 DEG C, ultrasound intensity 1.2W/cm3, obtain damp and hot rapeseed protein slurries；Tripolyphosphate is added into damp and hot rapeseed protein slurries
Sodium carries out ultrasonic wave added phosphatizing treatment 1.5 hours, and the additional amount of sodium tripolyphosphate is the 1.5 of damp and hot rapeseed protein slurries dosage
Times, phosphatizing treatment temperature is 45 DEG C, ultrasound intensity 1.2W/cm3, to the end of phosphatizing treatment, phosphorylation is obtained through dialysis
Rapeseed protein slurries；The pH to 10 of phosphorylation rapeseed protein slurries is adjusted, and alkali protease is added and carries out ultrasonic wave added enzymatic hydrolysis,
The additional amount of alkali protease is the 5.0% of rapeseed protein quality, and enzymolysis temperature is 55 DEG C, and the enzyme digestion reaction time is 3 small
When, ultrasound intensity 1.4W/cm3, to the end of primary enzymolysis reaction, obtain primary enzymolysis liquid；Adjust primary enzymolysis liquid pH to
6, and compound fertilizer production is added and carries out ultrasonic wave added enzymatic hydrolysis, the additional amount of compound fertilizer production is rapeseed protein quality
5.0%, enzymolysis temperature is 55 DEG C, and the enzyme digestion reaction time is 3 hours, ultrasound intensity 1.2W/cm3, reacted to secondary enzymolysis
Terminate, obtains secondary enzymatic solution；Microwave deactivating enzyme processing, microwave power 500W, microwave deactivating enzyme temperature are carried out to secondary enzymatic solution
It is 90 DEG C, the microwave deactivating enzyme time is 3 minutes, to the end of microwave deactivating enzyme, obtains enzyme deactivation enzymolysis liquid；Enzyme deactivation enzymolysis liquid is centrifuged, is taken
Supernatant obtains rapeseed peptides clear liquid, then more to get organic vegetable seed of finished product to rapeseed peptides clear liquid progress freeze-drying process
Peptide, the water imbibition of organic rapeseed peptides is 1.46g/g, oil absorption 1.46g/g, foaming characteristic 38.4%.
Embodiment 3:
It is impregnated after vegetable seed is peeled using n-hexane, obtains degreasing rapeseed dregs after removing solvent, resid amount 0.8% will
Degreasing rapeseed dregs crushed 40 meshes to get rapeseed dregs powder raw material；The acetone that rapeseed dregs powder raw material and concentration are 75% is pressed into feed liquid
It is mixed than 10:1, and carries out ultrasound-assisted extraction 25 minutes, extraction temperature is 48 DEG C, ultrasound intensity 1.2W/cm3, wait extract
End is taken, extraction rapeseed dregs powder is dried to obtain；The ethyl alcohol that rapeseed dregs powder and concentration are 90% will be extracted again to mix by solid-liquid ratio 8:1,
And carrying out ultrasound-assisted extraction 25 minutes, extraction temperature is 70 DEG C, ultrasound intensity 1.4W/cm3, end to be extracted, drying
Obtain virus-free rapeseed meal powder；Rapeseed dregs powder slurries are made by solid-liquid ratio 1:13 mixing in virus-free rapeseed meal powder and distilled water, are adjusted
Rapeseed dregs powder slurries pH to 11, and carry out ultrasonic wave added alkali soluble and handle 1 hour, alkali soluble temperature is 55 DEG C, ultrasound intensity 1.1W/
cm3, to alkali soluble, processing terminate, and centrifugation obtains supernatant liquor, and it adjusts supernatant liquor pH to 4.5 and carries out the heavy processing of acid 1.2 hours, to
Acid is heavy, and processing terminate, and centrifugation obtains precipitating, lower sediment is washed to neutrality, and carry out freeze-drying process to get arriving
Rapeseed protein, the water imbibition of the rapeseed protein is 2.62g/g, oil absorption 0.52g/g, emulsibility 59.4%；By vegetable seed
Albumen and distilled water are mixed by solid-liquid ratio 1:8, and carry out ultrasonic wave added humid heat treatment 30 minutes, and humid heat treatment temperature is 85 DEG C,
Ultrasound intensity is 1.0W/cm3, obtain damp and hot rapeseed protein slurries；Into damp and hot rapeseed protein slurries be added sodium tripolyphosphate into
Row ultrasonic wave added phosphatizing treatment 1.2 hours, the additional amount of sodium tripolyphosphate are 2.5 times of damp and hot rapeseed protein slurries dosage,
Phosphatizing treatment temperature is 50 DEG C, ultrasound intensity 1.3W/cm3, to the end of phosphatizing treatment, phosphorylation vegetable seed is obtained through dialysis
Protein slurry；The pH to 8 of phosphorylation rapeseed protein slurries is adjusted, and alkali protease is added and carries out ultrasonic wave added enzymatic hydrolysis, alkalinity
The additional amount of protease is the 5.5% of rapeseed protein quality, and enzymolysis temperature is 50 DEG C, and the enzyme digestion reaction time is 1 hour, is surpassed
Sound intensity degree is 1.6W/cm3, to the end of primary enzymolysis reaction, obtain primary enzymolysis liquid；The pH to 7 of primary enzymolysis liquid is adjusted, and is added
Entering compound fertilizer production and carries out ultrasonic wave added enzymatic hydrolysis, the additional amount of compound fertilizer production is the 5.5% of rapeseed protein quality,
Enzymolysis temperature is 45 DEG C, and the enzyme digestion reaction time is 4 hours, ultrasound intensity 1.3W/cm3, to the end of secondary enzymolysis reaction,
Obtain secondary enzymatic solution；Microwave deactivating enzyme processing, microwave power 800W are carried out to secondary enzymatic solution, microwave deactivating enzyme temperature is 85
DEG C, the microwave deactivating enzyme time is 2.5 minutes, to the end of microwave deactivating enzyme, obtains enzyme deactivation enzymolysis liquid；Enzyme deactivation enzymolysis liquid is centrifuged, is taken
Clear liquid obtains rapeseed peptides clear liquid, then carries out freeze-drying process to rapeseed peptides clear liquid to get organic rapeseed peptides of finished product,
The water imbibition of organic rapeseed peptides is 1.10g/g, oil absorption 1.13g/g, foaming characteristic 50.6%.
Although the embodiments of the present invention have been disclosed as above, but it is not limited in listed fortune in specification and embodiments
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily real
Now other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is not limited to
Specific details and legend shown and described herein.
Claims (5)
1. a kind of method that enzyme process prepares organic rapeseed peptides characterized by comprising
Step 1: the preparation of rapeseed protein: rapeseed dregs powder raw material being carried out detoxification treatment, virus-free rapeseed meal powder is obtained, by detoxification
Rapeseed dregs powder and distilled water are mixed by solid-liquid ratio 1:12~15 is made rapeseed dregs powder slurries, and adjusting rapeseed dregs powder slurries pH to 11~
12, and carry out ultrasonic wave added alkali soluble and handle 1~2 hour, alkali soluble temperature is 45~55 DEG C, and ultrasound intensity is 1.0~1.2W/cm3,
To alkali soluble, processing terminate, and centrifugation obtains supernatant liquor, adjusts supernatant liquor pH to 4.5 and carries out the heavy processing of acid 1.0~1.5 hours,
To acid it is heavy processing terminate, centrifugation obtains precipitating, lower sediment is washed to neutrality, and carry out freeze-drying process to get
To rapeseed protein, the water imbibition of the rapeseed protein is 1.03~4.21g/g, oil absorption is 0.52~2.37g/g, emulsibility is
32.4~59.4%；
Wherein, the process of the detoxification treatment is as follows: the acetone that rapeseed dregs powder raw material and concentration are 70%~80% is pressed feed liquid
It is mixed than 10~15:1, and carries out ultrasound-assisted extraction 15~25 minutes, extraction temperature is 45~50 DEG C, and ultrasound intensity is
1.2~1.4W/cm3, end to be extracted is dried to obtain extraction rapeseed dregs powder；Again will extraction rapeseed dregs powder and concentration be 80%~
90% ethyl alcohol is mixed by 8~12:1 of solid-liquid ratio, and carries out ultrasound-assisted extraction 20~30 minutes, and extraction temperature is 60~70
DEG C, ultrasound intensity is 1.4~1.6W/cm3, end to be extracted is dried to obtain virus-free rapeseed meal powder；
Step 2: humid heat treatment: rapeseed protein and distilled water being mixed by solid-liquid ratio 1:8~12, and it is damp and hot to carry out ultrasonic wave added
Processing 20~30 minutes, humid heat treatment temperature are 80~90 DEG C, and ultrasound intensity is 1.0~1.2W/cm3, obtain damp and hot vegetable seed egg
White slurries；
Step 3: phosphatizing treatment: sodium tripolyphosphate is added into damp and hot rapeseed protein slurries and carries out at ultrasonic wave added phosphorylation
Reason 1~1.5 hour, the additional amount of sodium tripolyphosphate are 1.5~2.5 times of damp and hot rapeseed protein slurries dosage, phosphatizing treatment
Temperature is 40~50 DEG C, and ultrasound intensity is 1.2~1.4W/cm3, to the end of phosphatizing treatment, phosphorylation vegetable seed is obtained through dialysis
Protein slurry；
Step 4: primary enzymolysis: adjusting the pH and temperature of phosphorylation rapeseed protein slurries, and alkali protease is added and carries out ultrasound
Assistance enzymolysis obtains primary enzymolysis liquid；
Step 5: secondary enzymolysis: adjusting the pH and temperature of primary enzymolysis liquid, and compound fertilizer production is added and carries out ultrasonic wave added
Enzymatic hydrolysis, obtains secondary enzymatic solution；
Step 6: microwave deactivating enzyme: carrying out microwave deactivating enzyme processing to secondary enzymatic solution, enzyme deactivation enzymolysis liquid is obtained, by enzyme deactivation enzymolysis liquid
Centrifugation obtains rapeseed peptides clear liquid, then carries out freeze-drying process to rapeseed peptides clear liquid to get organic rapeseed peptides of finished product.
2. the method that enzyme process as described in claim 1 prepares organic rapeseed peptides, which is characterized in that the rapeseed dregs powder raw material
Preparation process it is as follows: after vegetable seed is peeled using n-hexane impregnate, remove solvent after obtain degreasing rapeseed dregs, resid amount is
0.7~0.9%, degreasing rapeseed dregs be crushed into 40~80 meshes to get rapeseed dregs powder raw material.
3. the method that enzyme process as described in claim 1 prepares organic rapeseed peptides, which is characterized in that primary in the step 4
The process of enzymatic hydrolysis is as follows: adjusting the pH to 8~10 of phosphorylation rapeseed protein slurries, and alkali protease is added and carries out ultrasonic wave added
Enzymatic hydrolysis, the additional amount of alkali protease are the 4.5~5.5% of rapeseed protein quality, and enzymolysis temperature is 50~60 DEG C, enzymatic hydrolysis
Reaction time is 1~3 hour, and ultrasound intensity is 1.4~1.6W/cm3, to the end of primary enzymolysis reaction, obtain primary enzymolysis liquid.
4. the method that enzyme process as described in claim 1 prepares organic rapeseed peptides, which is characterized in that secondary in the step 5
The process of enzymatic hydrolysis is as follows: the pH to 5~7 of primary enzymolysis liquid adjusted, and compound fertilizer production is added and carries out ultrasonic wave added enzymatic hydrolysis,
The additional amount of compound fertilizer production is the 4.5~5.5% of rapeseed protein quality, and enzymolysis temperature is 45~55 DEG C, enzymatic hydrolysis
Reaction time is 2~4 hours, and ultrasound intensity is 1.2~1.4W/cm3, to the end of secondary enzymolysis reaction, obtain secondary enzymatic solution.
5. the method that enzyme process as described in claim 1 prepares organic rapeseed peptides, which is characterized in that microwave in the step 6
The process of enzyme deactivation is as follows: carrying out microwave deactivating enzyme processing to secondary enzymatic solution, microwave power is 500~800W, microwave deactivating enzyme temperature
It is 80~90 DEG C, the microwave deactivating enzyme time is 2~3 minutes, to the end of microwave deactivating enzyme, obtains enzyme deactivation enzymolysis liquid；By enzyme deactivation enzymolysis liquid
Centrifugation takes supernatant to obtain rapeseed peptides clear liquid, then carries out freeze-drying process to rapeseed peptides clear liquid to get the organic of finished product
Rapeseed peptides, the water imbibition of organic rapeseed peptides is 0.74~1.46g/g, oil absorption is 1.13~1.78g/g, foaming characteristic
It is 38.4~50.6%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610225648.8A CN105779542B (en) | 2016-04-13 | 2016-04-13 | A kind of method that enzyme process prepares organic rapeseed peptides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610225648.8A CN105779542B (en) | 2016-04-13 | 2016-04-13 | A kind of method that enzyme process prepares organic rapeseed peptides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105779542A CN105779542A (en) | 2016-07-20 |
| CN105779542B true CN105779542B (en) | 2019-03-08 |
Family
ID=56396368
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610225648.8A Active CN105779542B (en) | 2016-04-13 | 2016-04-13 | A kind of method that enzyme process prepares organic rapeseed peptides |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105779542B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107772077A (en) * | 2016-08-30 | 2018-03-09 | 中粮营养健康研究院有限公司 | A kind of method of the protein solubility of rape cake and raising rape cake |
| CN107400158B (en) * | 2017-09-28 | 2021-04-30 | 南京财经大学 | Method for preparing high-yield anti-inflammatory rapeseed peptides through step-by-step enzymolysis |
| CN107502645B (en) * | 2017-10-12 | 2021-04-13 | 海南保亭椰泽坊食品有限公司 | Preparation method of coconut polypeptide |
| CN107502644A (en) * | 2017-10-12 | 2017-12-22 | 南京财经大学 | A kind of isolation and purification method of vegetable seed anti-inflammatory peptides |
| CN107960526A (en) * | 2017-11-27 | 2018-04-27 | 余雪平 | Collagen protein powder and its production technology |
| CN108866138A (en) * | 2018-08-29 | 2018-11-23 | 宝得瑞（湖北）健康产业有限公司 | A kind of preparation method of pumpkin polypeptide |
| CN115644362A (en) * | 2022-11-11 | 2023-01-31 | 湖南省农产品加工研究所 | Low-salt fermented bean curd and preparation method thereof |
| CN116268344A (en) * | 2023-03-06 | 2023-06-23 | 鼎新思远重庆粮油食品工业有限公司 | Process method for producing edible rapeseed powder and automatic extractor |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102643889A (en) * | 2012-04-28 | 2012-08-22 | 南京财经大学 | Antihypertensive active rapeseed peptide and preparation method and application thereof |
| CN104109706A (en) * | 2014-07-24 | 2014-10-22 | 南京财经大学 | Method for separating and purifying antioxidant peptide of rapeseed |
-
2016
- 2016-04-13 CN CN201610225648.8A patent/CN105779542B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102643889A (en) * | 2012-04-28 | 2012-08-22 | 南京财经大学 | Antihypertensive active rapeseed peptide and preparation method and application thereof |
| CN104109706A (en) * | 2014-07-24 | 2014-10-22 | 南京财经大学 | Method for separating and purifying antioxidant peptide of rapeseed |
Non-Patent Citations (2)
| Title |
|---|
| 不同预处理方法对菜籽蛋白酶解效果的影响;王微星等;《浙江农业学报》;20121231;第24卷(第5期);第2.3节 |
| 菜籽蛋白酶解及产物抗氧化活性的研究;金晶;《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》;20111231(第S1期);摘要、第1.1-1.2、2.1.1、2.1.3、2.2.1.2、2.2.1.5节 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105779542A (en) | 2016-07-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105779542B (en) | A kind of method that enzyme process prepares organic rapeseed peptides | |
| CN103704462B (en) | Extraction and purification method for sesame proteins | |
| CN104206641B (en) | A kind of giant salamander polypeptide powder and preparation method thereof | |
| CN105349246A (en) | Method for synchronous extraction of grease and protein peptide from shiny-leaved yellowhorn | |
| CN105410949B (en) | A kind of method that extraction prepares bamboo shoot dietary fiber in leftover bits and pieces from bamboo shoot | |
| CN104126669A (en) | Method for increasing product yield of bean product | |
| CN103014108A (en) | Preparation method of corn oligopeptide | |
| CN107557420A (en) | A kind of preparation method of flaxseed meal polypeptide | |
| CN102329382A (en) | Method for extracting rapeseed proteins through ultrasonic-microwave synergy | |
| CN104140471A (en) | Method for preparing yam high purity polysaccharide from yam food processing leftovers | |
| CN100591226C (en) | Method for extracting composite amino acid from silkworm pupa by biological enzyme method | |
| CN107586600A (en) | A kind of preparation method of natural coconut oil | |
| CN109111497A (en) | A kind of method for producing of peony seeds albumen | |
| CN106723026A (en) | A kind of Barley Greeg and its preparation technology | |
| CN109122883A (en) | A kind of polypeptide strengthens the production method of instant mung bean milk powder | |
| CN104531814B (en) | A kind of preparation method of Extracted From Oil-tea-cake enzyme modification albumen | |
| CN107502645A (en) | A kind of preparation method of coconut polypeptide | |
| CN105063149B (en) | A kind of method that enzyme process auxiliary prepares cottonseed protein | |
| CN106666071A (en) | Method for preparing flavor peptide from vegetable protein | |
| CN105876076A (en) | Method for preparing bone polypeptides through microwave-assisted enzymolysis | |
| CN103518943A (en) | Method for extracting proteins from rape seed cakes by utilizing multi-frequency ultrasonic waves | |
| CN111363619A (en) | Method for synchronously preparing peony seed cold-pressed oil and protein by enzyme method | |
| CN111066942A (en) | Preparation method of taste-presenting peptide by taking peanut meal as raw material | |
| CN105255585A (en) | Preparation method of xanthoceras sorbifolia bunge oil and xanthoceras sorbifolia bunge protein peptide | |
| CN106689641A (en) | Production technology of walnut peptide powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |