CN105349246A - Method for synchronous extraction of grease and protein peptide from shiny-leaved yellowhorn - Google Patents
Method for synchronous extraction of grease and protein peptide from shiny-leaved yellowhorn Download PDFInfo
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- CN105349246A CN105349246A CN201510769225.8A CN201510769225A CN105349246A CN 105349246 A CN105349246 A CN 105349246A CN 201510769225 A CN201510769225 A CN 201510769225A CN 105349246 A CN105349246 A CN 105349246A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a method for synchronous extraction of grease and a protein peptide from shiny-leaved yellowhorn, and belongs to the technical field of plant grease and protein processing. The method includes the following steps: (1) peeling shiny-leaved yellowhorn, then crushing, and next carrying out vacuum extrusion expanding treatment, to obtain an expanded product; (2) adding water in the expanded product and mixing, then adding protease, carrying out magnetic field strengthening enzymolysis, and after enzymolysis, carrying out centrifugal separation to obtain free oil, a hydrolysate, an emulsion and residues; (3) mixing the emulsion with water, then adding phospholipase, carrying out ultrasonic enzymolysis demulsification, and after demulsification, carrying out centrifugal separation to obtain free oil and a hydrolysate; (4) merging the hydrolysates, then adjusting a pH to be neutral, then successively carrying out ultrafiltration treatment and nanofiltration treatment, and carrying out spray drying of the filtrate to obtain a protein peptide powder; and (5) washing the residues, carrying out vacuum drying, and crushing to obtain an animal feed. The shiny-leaved yellowhorn oil prepared by the method has high extraction rate and good quality, and the shiny-leaved yellowhorn protein peptide having high purity and strong activity also can be obtained at the same time and can be applied to industrial production with certain scale.
Description
Technical field
The invention belongs to Vegetable oil lipoprotein and Protein processing technical field, relate generally to a kind of method of simultaneous extraction grease and protein peptide from Wood of Shinyleaf Yellowhorn.
Background technology
Wood of Shinyleaf Yellowhorn has higher nutritive value, seed oil length is 30.4%, benevolence oil length is planted up to 66.39% after peeling, wherein unsaturated fatty acid content is up to 94%, linolic acid accounts for 36.9%, oleic acid accounts for 57.16%, higher than sweet oil, soybean oil nutritive value, can the effective illness such as preventing hypertension, hyperlipidemia, arteriosclerosis.In addition, the protein also containing 25.75% in Wood of Shinyleaf Yellowhorn is the vegetable-protein that a kind of nutritive value is higher, and wherein containing abundant amino acid, amino acid ratio is balanced, is better than Semen arachidis hypogaeae protein, and Wood of Shinyleaf Yellowhorn albumen also has functional preferably simultaneously.
Method mainly milling process and the extraction of current prepairng vegetable oil.Milling process technique is simple, and support equipment is few, can keep the flavour of product, but oil extracting rate is low, energy consumption is large, cost is high, and cake protein qualitative change after squeezing is serious, and protein utilization rate is low, causes the serious waste of vegetable-protein.Lixiviation process production efficiency is high and residual oil content is low, but this method equipment is many, investment is large, and oil product flavor loss is large and have organic solvent residual, also needs refining treatment, also pollutes the environment simultaneously.
Protein polypeptide is the protein fragments that two or more amino acid are connected into by peptide bond, and it is the ultimate constituent of body tissue cell.Since the importance of biologically active peptides in food and healthcare products is familiar with, the research that employing enzyme process extracts biologically active peptides from plant resources is also more and more extensive.Wood of Shinyleaf Yellowhorn protein peptide has important physiological function, and it can remove interior free yl, reaches anti-oxidant, extends the effect of cell survival.Simultaneously its property digested and assimilated is good, food safety is high, therefore can be used as nutrition-fortifying agent and be applied in immunoenzyme technics formula food, diet food, arsenic and dietetic food.
Aqueous enzymatic method carry oil tech be occur for 20 end of the centurys a kind of green, environmental protection, healthy vegetable oil extraction new technology, receive the extensive concern of countries in the world scholar.Compared with traditional oil extracting process, it is low that aqueous enzymatic method puies forward oily energy consumption, environmental protection, and action condition is gentle, and avoid the use of organic solvent, the grease of extraction is without the need to refining.In addition, aqueous enzymatic method, while extraction Vegetable oil lipoprotein, can obtain protein or the protolysate peptide of high-quality, be a kind of have an application prospect put forward oily leach protein technology.
Summary of the invention
Technical problem to be solved by this invention overcomes the deficiencies in the prior art, provides a kind of method of simultaneous extraction grease and protein peptide from Wood of Shinyleaf Yellowhorn, reach Simplified flowsheet, enhance productivity, improves the object of grease and protein quality.
Technical problem to be solved by this invention is achieved through the following technical solutions:
A kind of method of simultaneous extraction grease and protein peptide from Wood of Shinyleaf Yellowhorn, the method comprises the following steps: (1) pulverizes after Wood of Shinyleaf Yellowhorn decortication, then regulate moisture to carry out vacuum extrusion puffing and obtain expanded product, described vacuum tightness is 0.03-0.07MPa, material water ratio is 12-16%, jacket temperature is 90-110 DEG C, and screw speed is 80-120r/min; (2) expanded product is added water be mixed to get mixed solution, in mixed solution, add Sumizyme MP and be placed in magnetic field and carry out magnetic field-intensification enzymolysis, described magneticstrength is 200-600mT, proteolytic enzyme addition is the 0.5-2% of mixed solution quality, hydrolysis temperature is 45-65 DEG C, enzymolysis pH is 8-10, and enzymolysis time is 1-3h, and after enzymolysis, centrifugation obtains free oil, hydrolyzed solution, milk sap, residue; (3) milk sap and equal-volume water are mixed to get mixed emulsion, Phospholipid hydrolase is added in mixed emulsion, and be placed in ultrasonic reactor and carry out ultrasonic enzymolysis breakdown of emulsion, described ultrasonic power is 100-200W, Phospholipid hydrolase addition is the 1-3% of mixed emulsion quality, and hydrolysis temperature is 40-60 DEG C, and enzymolysis pH is 5-7, enzymolysis time is 40-80min, and after breakdown of emulsion, centrifugation obtains free oil and hydrolyzed solution; (4) regulate pH to neutral after being merged by the hydrolyzed solution obtained in step (2) and step (3), then carry out ultrafiltration and nanofiltration process successively, filtrate is carried out spraying dry and obtains protein peptide powder; (5) residue is washed with water to neutrality, then carries out vacuum-drying, pulverize to obtain animal-feed.
The expanded preferred parameter of described vacuum extrusion is: vacuum tightness 0.05MPa, material water ratio 15%, jacket temperature 105 DEG C, screw speed 90r/min.
Described magnetic field-intensification enzymolysis preferred parameter is: magneticstrength 400mT, and proteolytic enzyme addition is 1% of mixed solution quality, hydrolysis temperature 55 DEG C, enzymolysis pH9.5, enzymolysis time 2h.
Described ultrasonic enzymolysis breakdown of emulsion preferred parameter is: ultrasonic power 150W, and Phospholipid hydrolase addition is 1.5% of mixed emulsion quality, hydrolysis temperature 50 DEG C, enzymolysis pH6, enzymolysis time 60min.
Present method for raw material, adopts that vacuum extrusion is expanded carries out pre-treatment with the Wood of Shinyleaf Yellowhorn of decortication, destroys cellularstructure, makes protein wherein that suitable sex change occur, expose enzymolysis site, be beneficial to enzymolysis.Then under the action of a magnetic field, carry out magnetic field-intensification enzymolysis, the efficiency of enzyme digestion reaction, by changing the conformational forms at enzyme molecular activity position to improve the vigor of enzyme, is improved in magnetic field simultaneously.Carry out ultrasonic wave added Phospholipid hydrolase breakdown of emulsion to milk sap after enzymolysis and reclaim shinyleaf yellowhorn oil, the mode of hydrolyzed solution being carried out to membrane filtration reclaims Wood of Shinyleaf Yellowhorn protein peptide.The method has the advantages that technique is simple, cost is low, the high and quality better of shinyleaf yellowhorn oil extraction yield, can also obtain the high and active strong Wood of Shinyleaf Yellowhorn protein peptide of purity simultaneously, can be applicable to the industrial production of certain scale.
Embodiment
A kind of method of simultaneous extraction grease and protein peptide from Wood of Shinyleaf Yellowhorn, the method comprises the following steps: (1) pulverizes after Wood of Shinyleaf Yellowhorn decortication, then regulate moisture to carry out vacuum extrusion puffing and obtain expanded product, described vacuum tightness is 0.03-0.07MPa, material water ratio is 12-16%, jacket temperature is 90-110 DEG C, and screw speed is 80-120r/min; (2) expanded product is added water be mixed to get mixed solution, in mixed solution, add Sumizyme MP and be placed in magnetic field and carry out magnetic field-intensification enzymolysis, described magneticstrength is 200-600mT, proteolytic enzyme addition is the 0.5-2% of mixed solution quality, hydrolysis temperature is 45-65 DEG C, enzymolysis pH is 8-10, and enzymolysis time is 1-3h, and after enzymolysis, centrifugation obtains free oil, hydrolyzed solution, milk sap, residue; (3) milk sap and equal-volume water are mixed to get mixed emulsion, Phospholipid hydrolase is added in mixed emulsion, and be placed in ultrasonic reactor and carry out ultrasonic enzymolysis breakdown of emulsion, described ultrasonic power is 100-200W, Phospholipid hydrolase addition is the 1-3% of mixed emulsion quality, and hydrolysis temperature is 40-60 DEG C, and enzymolysis pH is 5-7, enzymolysis time is 40-80min, and after breakdown of emulsion, centrifugation obtains free oil and hydrolyzed solution; (4) regulate pH to neutral after being merged by the hydrolyzed solution obtained in step (2) and step (3), then carry out ultrafiltration and nanofiltration process successively, filtrate is carried out spraying dry and obtains protein peptide powder; (5) residue is washed with water to neutrality, then carries out vacuum-drying, pulverize to obtain animal-feed.
The expanded preferred parameter of described vacuum extrusion is: vacuum tightness 0.05MPa, material water ratio 15%, jacket temperature 105 DEG C, screw speed 90r/min.
Described magnetic field-intensification enzymolysis preferred parameter is: magneticstrength 400mT, and proteolytic enzyme addition is 1% of mixed solution quality, hydrolysis temperature 55 DEG C, enzymolysis pH9.5, enzymolysis time 2h.
Described ultrasonic enzymolysis breakdown of emulsion preferred parameter is: ultrasonic power 150W, and Phospholipid hydrolase addition is 1.5% of mixed emulsion quality, hydrolysis temperature 50 DEG C, enzymolysis pH6, enzymolysis time 60min.
Embodiment 1:
Pulverize after Wood of Shinyleaf Yellowhorn decortication, then moisture is regulated, vacuum tightness be 0.05MPa, material water ratio is 15%, jacket temperature is 105 DEG C, screw speed carries out vacuum extrusion puffing under being 90r/min condition and obtains expanded product, expanded product is added water and is mixed to get mixed solution, in mixed solution, add the Sumizyme MP of 1% and be placed in magnetic field, magneticstrength be 400mT, hydrolysis temperature is 55 DEG C, enzymolysis pH carries out magnetic field-intensification enzymolysis 2h under being 9.5 conditions, after enzymolysis, centrifugation obtains free oil, hydrolyzed solution, milk sap, residue; Milk sap and equal-volume water are mixed to get mixed emulsion, in mixed emulsion, add the Phospholipid hydrolase of 1.5% and be placed in ultrasonic reactor, ultrasonic power be 150W, hydrolysis temperature is 50 DEG C, enzymolysis pH carries out ultrasonic enzymolysis breakdown of emulsion 60min under being 6 conditions, after breakdown of emulsion, centrifugation obtains free oil and hydrolyzed solution, regulate pH to neutral after merging twice hydrolyzed solution, then ultrafiltration and nanofiltration process is carried out successively, filtrate is carried out spraying dry and obtains protein peptide powder, residue is washed with water to neutrality, then carries out vacuum-drying, pulverize to obtain animal-feed.The method technique is simple, cost is low, and shinyleaf yellowhorn oil extraction yield up to 95.56% and quality better, can also obtain the high and active strong Wood of Shinyleaf Yellowhorn protein peptide of purity simultaneously, can be applicable to the industrial production of certain scale.
Embodiment 2:
Pulverize after Wood of Shinyleaf Yellowhorn decortication, then moisture is regulated, vacuum tightness be 0.06MPa, material water ratio is 14%, jacket temperature is 110 DEG C, screw speed carries out vacuum extrusion puffing under being 100r/min condition and obtains expanded product, expanded product is added water and is mixed to get mixed solution, in mixed solution, add the Sumizyme MP of 1.5% and be placed in magnetic field, magneticstrength be 300mT, hydrolysis temperature is 50 DEG C, enzymolysis pH carries out magnetic field-intensification enzymolysis 1.5h under being 9 conditions, after enzymolysis, centrifugation obtains free oil, hydrolyzed solution, milk sap, residue; Milk sap and equal-volume water are mixed to get mixed emulsion, in mixed emulsion, add the Phospholipid hydrolase of 2% and be placed in ultrasonic reactor, ultrasonic power be 100W, hydrolysis temperature is 55 DEG C, enzymolysis pH carries out ultrasonic enzymolysis breakdown of emulsion 50min under being 5.5 conditions, after breakdown of emulsion, centrifugation obtains free oil and hydrolyzed solution, regulate pH to neutral after merging twice hydrolyzed solution, then ultrafiltration and nanofiltration process is carried out successively, filtrate is carried out spraying dry and obtains protein peptide powder, residue is washed with water to neutrality, then carries out vacuum-drying, pulverize to obtain animal-feed.The method technique is simple, cost is low, and shinyleaf yellowhorn oil extraction yield up to 94.25% and quality better, can also obtain the high and active strong Wood of Shinyleaf Yellowhorn protein peptide of purity simultaneously, can be applicable to the industrial production of certain scale.
Embodiment 3:
Pulverize after Wood of Shinyleaf Yellowhorn decortication, then moisture is regulated, vacuum tightness be 0.04MPa, material water ratio is 15%, jacket temperature is 100 DEG C, screw speed carries out vacuum extrusion puffing under being 90r/min condition and obtains expanded product, expanded product is added water and is mixed to get mixed solution, in mixed solution, add the Sumizyme MP of 1% and be placed in magnetic field, magneticstrength be 500mT, hydrolysis temperature is 50 DEG C, enzymolysis pH carries out magnetic field-intensification enzymolysis 2.5h under being 10 conditions, after enzymolysis, centrifugation obtains free oil, hydrolyzed solution, milk sap, residue; Milk sap and equal-volume water are mixed to get mixed emulsion, in mixed emulsion, add the Phospholipid hydrolase of 1% and be placed in ultrasonic reactor, ultrasonic power be 200W, hydrolysis temperature is 55 DEG C, enzymolysis pH carries out ultrasonic enzymolysis breakdown of emulsion 60min under being 5.5 conditions, after breakdown of emulsion, centrifugation obtains free oil and hydrolyzed solution, regulate pH to neutral after merging twice hydrolyzed solution, then ultrafiltration and nanofiltration process is carried out successively, filtrate is carried out spraying dry and obtains protein peptide powder, residue is washed with water to neutrality, then carries out vacuum-drying, pulverize to obtain animal-feed.The method technique is simple, cost is low, and shinyleaf yellowhorn oil extraction yield up to 94.81% and quality better, can also obtain the high and active strong Wood of Shinyleaf Yellowhorn protein peptide of purity simultaneously, can be applicable to the industrial production of certain scale.
Claims (4)
1. the method for simultaneous extraction grease and protein peptide from a Wood of Shinyleaf Yellowhorn, it is characterized in that the method comprises the following steps: (1) pulverizes after Wood of Shinyleaf Yellowhorn decortication, then regulate moisture to carry out vacuum extrusion puffing and obtain expanded product, described vacuum tightness is 0.03-0.07MPa, material water ratio is 12-16%, jacket temperature is 90-110 DEG C, and screw speed is 80-120r/min; (2) expanded product is added water be mixed to get mixed solution, in mixed solution, add Sumizyme MP and be placed in magnetic field and carry out magnetic field-intensification enzymolysis, described magneticstrength is 200-600mT, proteolytic enzyme addition is the 0.5-2% of mixed solution quality, hydrolysis temperature is 45-65 DEG C, enzymolysis pH is 8-10, and enzymolysis time is 1-3h, and after enzymolysis, centrifugation obtains free oil, hydrolyzed solution, milk sap, residue; (3) milk sap and equal-volume water are mixed to get mixed emulsion, Phospholipid hydrolase is added in mixed emulsion, and be placed in ultrasonic reactor and carry out ultrasonic enzymolysis breakdown of emulsion, described ultrasonic power is 100-200W, Phospholipid hydrolase addition is the 1-3% of mixed emulsion quality, and hydrolysis temperature is 40-60 DEG C, and enzymolysis pH is 5-7, enzymolysis time is 40-80min, and after breakdown of emulsion, centrifugation obtains free oil and hydrolyzed solution; (4) regulate pH to neutral after being merged by the hydrolyzed solution obtained in step (2) and step (3), then carry out ultrafiltration and nanofiltration process successively, filtrate is carried out spraying dry and obtains protein peptide powder; (5) residue is washed with water to neutrality, then carries out vacuum-drying, pulverize to obtain animal-feed.
2. the method for a kind of simultaneous extraction grease and protein peptide from Wood of Shinyleaf Yellowhorn according to claim 1, it is characterized in that the expanded preferred parameter of described vacuum extrusion is: vacuum tightness 0.05MPa, material water ratio 15%, jacket temperature 105 DEG C, screw speed 90r/min.
3. the method for a kind of simultaneous extraction grease and protein peptide from Wood of Shinyleaf Yellowhorn according to claim 1, it is characterized in that described magnetic field-intensification enzymolysis preferred parameter is: magneticstrength 400mT, proteolytic enzyme addition is 1% of mixed solution quality, hydrolysis temperature 55 DEG C, enzymolysis pH9.5, enzymolysis time 2h.
4. the method for a kind of simultaneous extraction grease and protein peptide from Wood of Shinyleaf Yellowhorn according to claim 1, the method of its special a kind of simultaneous extraction grease and protein peptide from Wood of Shinyleaf Yellowhorn according to claim 1, it is characterized in that described ultrasonic enzymolysis breakdown of emulsion preferred parameter is: ultrasonic power 150W, Phospholipid hydrolase addition is 1.5% of mixed emulsion quality, hydrolysis temperature 50 DEG C, enzymolysis pH6, enzymolysis time 60min.
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Cited By (13)
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| CN105925365A (en) * | 2016-05-17 | 2016-09-07 | 东北农业大学 | Method for extracting soybean oil and non-bitter soybean peptide through aqueous enzymatic method |
| CN105950275A (en) * | 2016-05-17 | 2016-09-21 | 东北农业大学 | Aqueous enzymatic method for preparing blend sesame oil and composite protein peptide |
| CN106173651A (en) * | 2016-07-09 | 2016-12-07 | 东北农业大学 | A kind of Semen sojae atricolor is for the preparation method of food and drink material |
| CN106755233A (en) * | 2016-12-05 | 2017-05-31 | 东北农业大学 | A kind of method that aqueous enzymatic method Soy hydrolysate prepares protein peptide powder |
| CN106978459A (en) * | 2017-04-12 | 2017-07-25 | 山西双林富农林开发有限公司 | A kind of shiny-leaved yellowhorn protein peptides and preparation method thereof, protein peptide health product |
| CN106986917A (en) * | 2017-04-12 | 2017-07-28 | 岭南师范学院 | One kind method of separating protein from tea oil oil reservoir |
| CN107216943A (en) * | 2017-07-31 | 2017-09-29 | 长沙爱扬医药科技有限公司 | Utilize the method for wilson dogwood fruit wilson dogwood oil |
| CN110862862A (en) * | 2019-11-28 | 2020-03-06 | 内蒙古农业大学 | Method for simultaneously preparing xanthoceras sorbifolia Bunge oil and xanthoceras sorbifolia Bunge protein by aqueous enzymatic method |
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| CN105925365A (en) * | 2016-05-17 | 2016-09-07 | 东北农业大学 | Method for extracting soybean oil and non-bitter soybean peptide through aqueous enzymatic method |
| CN105950275A (en) * | 2016-05-17 | 2016-09-21 | 东北农业大学 | Aqueous enzymatic method for preparing blend sesame oil and composite protein peptide |
| CN106173651A (en) * | 2016-07-09 | 2016-12-07 | 东北农业大学 | A kind of Semen sojae atricolor is for the preparation method of food and drink material |
| CN106755233A (en) * | 2016-12-05 | 2017-05-31 | 东北农业大学 | A kind of method that aqueous enzymatic method Soy hydrolysate prepares protein peptide powder |
| CN106978459A (en) * | 2017-04-12 | 2017-07-25 | 山西双林富农林开发有限公司 | A kind of shiny-leaved yellowhorn protein peptides and preparation method thereof, protein peptide health product |
| CN106986917A (en) * | 2017-04-12 | 2017-07-28 | 岭南师范学院 | One kind method of separating protein from tea oil oil reservoir |
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| CN110862862A (en) * | 2019-11-28 | 2020-03-06 | 内蒙古农业大学 | Method for simultaneously preparing xanthoceras sorbifolia Bunge oil and xanthoceras sorbifolia Bunge protein by aqueous enzymatic method |
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