CN102080107A - Method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis - Google Patents
Method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis Download PDFInfo
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- CN102080107A CN102080107A CN201010585253.1A CN201010585253A CN102080107A CN 102080107 A CN102080107 A CN 102080107A CN 201010585253 A CN201010585253 A CN 201010585253A CN 102080107 A CN102080107 A CN 102080107A
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- 235000020234 walnut Nutrition 0.000 title claims abstract description 61
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 36
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 36
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 36
- 235000019498 Walnut oil Nutrition 0.000 title claims abstract description 32
- 239000008170 walnut oil Substances 0.000 title claims abstract description 32
- 108091005804 Peptidases Proteins 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 25
- 241000758789 Juglans Species 0.000 title claims abstract description 20
- 239000004365 Protease Substances 0.000 title claims abstract description 19
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title claims abstract description 16
- 230000007062 hydrolysis Effects 0.000 title claims abstract description 10
- 238000006460 hydrolysis reaction Methods 0.000 title claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000003921 oil Substances 0.000 claims abstract description 23
- 235000019198 oils Nutrition 0.000 claims abstract description 23
- 239000007790 solid phase Substances 0.000 claims abstract description 17
- 239000012071 phase Substances 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 102000004190 Enzymes Human genes 0.000 claims description 12
- 108090000790 Enzymes Proteins 0.000 claims description 12
- 102000035195 Peptidases Human genes 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 11
- 244000005700 microbiome Species 0.000 claims description 7
- 108010004032 Bromelains Proteins 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 6
- 235000012054 meals Nutrition 0.000 claims description 6
- 210000002784 stomach Anatomy 0.000 claims description 6
- 238000013019 agitation Methods 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 5
- 235000013325 dietary fiber Nutrition 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 108010059712 Pronase Proteins 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 229920001184 polypeptide Polymers 0.000 claims description 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
- 238000007670 refining Methods 0.000 claims description 3
- 101800000068 Antioxidant peptide Proteins 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 claims description 2
- 244000184861 Juglans nigra Species 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 claims description 2
- 239000000654 additive Substances 0.000 claims description 2
- 230000000996 additive effect Effects 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 239000002537 cosmetic Substances 0.000 claims description 2
- 238000005238 degreasing Methods 0.000 claims description 2
- 230000003203 everyday effect Effects 0.000 claims description 2
- -1 makeup Substances 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- 238000000108 ultra-filtration Methods 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 238000000227 grinding Methods 0.000 abstract description 2
- 239000008346 aqueous phase Substances 0.000 abstract 3
- 239000002131 composite material Substances 0.000 abstract 1
- 238000000874 microwave-assisted extraction Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- 239000002351 wastewater Substances 0.000 abstract 1
- 240000007049 Juglans regia Species 0.000 description 45
- 235000019419 proteases Nutrition 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 2
- 230000017854 proteolysis Effects 0.000 description 2
- 244000144730 Amygdalus persica Species 0.000 description 1
- 108090000145 Bacillolysin Proteins 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 235000013757 Juglans Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
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- 102000035092 Neutral proteases Human genes 0.000 description 1
- 108091005507 Neutral proteases Proteins 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 235000019495 Pecan oil Nutrition 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 229960004232 linoleic acid Drugs 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 239000010470 pecan oil Substances 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis, comprising the following steps of: grinding walnut raw materials into walnut powder, adding 100,000 to 1000,000 units of protease or composite protease to 1kg of walnut powder, adding water in the mass ratio of the walnut powder to the water is 1: (0.5-20), and then performing microwave processing for 1-10 minutes under the stirring condition of 60 to 300rpm when the microwave power is 10 to 1000 W/L and the temperature is 35 to 65 DEG C, and performing centrifugal separation of oil phase, aqueous phase and solid phase after the reaction ends, wherein the oil phase is walnut oil, the aqueous phase is walnut protein peptide, and the solid phase is residues. The method provided by the invention has mild extraction condition and excellent product performance, and can improve the efficiency of ingress of oil. Furthermore, the obtained walnut oil is higher in quality and is easy to refine; the processing time is greatly shortened by microwave-assisted extraction; the oil phase, the aqueous phase and the solid phase residues can be separated at the same time, therefore, the production efficiency is efficiently improved; and no waste water, waste air or waste residues exists in the producing process, therefore, the invention is environmentally-friendly.
Description
Technical field
The present invention relates to a kind of method for preparing walnut oil and walnut protein peptide, especially a kind of microwave-assisted protease hydrolysis prepares the method for walnut oil and walnut protein peptide synchronously, belongs to food or health food technology field.
Background technology
Walnut (
Juglans regia L.) call English walnut, Qiang peach, belong to the Juglandaceae walnut.Walnut kernel contain grease up to 65%, protein is more than 15.4%, other has phosphatide, multivitamin, and suitable linolic acid, the linolenic acid of abundant amount ratio.Walnut oil has very high medical and edibleness, and its preparation method has milling process, water band method, solvent extration etc.In recent years use aqueous enzymatic method and carry out the walnut oil extraction, the edible vegetable oil yield can reach 40%; 200510046309.5 of patents of invention at the decortication Semen Caryae Cathayensis (
Juglans mandshurca Maxlm) carrying out aqueous enzymatic extraction pecan oil, used enzyme is complex cellulase, proteolytic enzyme etc., needs 45～60 ℃ of enzymolysis 2～10 hours, enzymolysis time is long, easily grows microorganism.Use lipase in the patent of invention " the aqua single stage method is extracted walnut oil and proteic method from walnut kernel " (200910024007.6) and extracted walnut oil and protein, unavoidably walnut oil has been hydrolyzed to lipid acid.The patent of invention method of active polypeptide " the microwave-assisted enzymolysis oil meal protein produce " (200910062276.1) at raw material be grouts, zymin is neutral protease, Sumizyme MP and flavor protease, product only has bioactive peptide.So so far, do not see the report that the microwave-assisted proteolysis is studied and used as yet in walnut oil and the synchronous preparation technology of walnut protein peptide.
Summary of the invention
For overcoming preparation walnut oil and walnut protein peptide complex process, problems such as the walnut oil that obtains is of low quality, the invention provides the method that the microwave-assisted protease hydrolysis prepares walnut oil and walnut protein peptide synchronously, can under comparatively gentle condition, efficiently obtain walnut oil, walnut protein peptide, walnut diet fiber product fast, make its production technique reach economical and efficient and environmental friendliness.
The present invention realizes by following technical proposal: a kind of microwave-assisted protease hydrolysis prepares the method for walnut oil and walnut protein peptide synchronously, it is characterized in that through the following step:
A. walnut raw material mill is walnut mud;
B. in the walnut mud of per kilogram steps A, add proteolytic enzyme or compound protease 10～1,000,000 units, add entry and mix by walnut mud ︰ water=mass ratio of 1 ︰ 0.5～20;
C. under the agitation condition of 60～300rpm, in microwave power 10～1000W/L, temperature is under 35～65 ℃, mixture to step B carried out microwave treatment 1～10 minute, after reaction finishes, centrifugation is fuel-displaced, water, solid phase, wherein: oil phase is walnut oil, and water is walnut protein peptide, and solid phase is a residue.
The walnut raw material of described steps A is hulled walnut kernel or de-oiling walnut meal not.
Water among the described step B is pure water, deionized water or distilled water.
Proteolytic enzyme among the described step B is the biological peptide bond hydrolysis enzyme of animal, plant, microorganism, comprise papoid, Traumanase, stomach en-, trypsinase, perhaps derive from the pronase e of microorganism, one or more the mixing in the bacillus proteolytic enzyme.
The preferred papoid of proteolytic enzyme among the described step B, stomach en-, trypsinase.
Compound protease among the described step B is papoid, Traumanase, stomach en-, trypsinase, perhaps derive from the pronase e of microorganism, one or more the mixing in the bacillus proteolytic enzyme, mix like this to use and to shorten hydrolysis time, improve product yield and quality, so that product obtains mouthfeel or functional protein peptide preferably.
The residue of gained among the described step C is made dietary fiber food through conventional drying, pulverizing.
The walnut oil of gained among the described step C, directly edible or further through the stock oil of conventional low-temperature physics refining as table oil, health care oil, mixed oil.
The walnut protein peptide of gained among the described step C is handled through the routine enzyme that goes out, and is directly used in and produces degreasing walnut protein peptide drink; Or prepare walnut antioxidant peptide active polypeptide products through conventional ultrafiltration, be used for healthcare products, foodstuff additive, or nutrition-fortifying agent, makeup, cosmetics of everyday use; Or get the walnut protein peptide powder through conventional low temperature spray drying.
The described routine enzyme that goes out handle to adopt conventional heating means to come inactivated proteases, and the condition that the enzyme that goes out is handled is 90 ℃ of heating 5 minutes or boiled 3 minutes.
The present invention is a new integrated technique, by finish grinding, converting slurry, microwave-assisted proteolysis, makes walnut oil release from combine closely with walnut protein, thereby effectively improves the extraction efficiency of walnut oil and walnut protein peptide, increases the diversity of functional product.Extraction conditions of the present invention is relatively gentleer, and the product proterties is good, compares with traditional technology, has following tangible advantage:
1. equipment is simple, operational safety;
2. can improve oil yield efficiency, and resulting walnut oil quality is higher, is easy to refining;
3. enzymatic treatment mild condition;
4. extract by microwave-assisted, the treatment time shortens greatly;
5. easily separated, can separate oil phase, water and solid phase residue simultaneously, can enhance productivity greatly;
6. production process does not have the three wastes, for environmentally friendly.
Embodiment
Below in conjunction with embodiment the present invention is described further.
Embodiment 1
A. the hulled walnut kernel mill is walnut mud;
B. get the walnut mud of 1kg steps A, and add the papoid of 100,000 units therein, add pure water 0.5kg again and mix;
C. under the agitation condition of 60rpm, at microwave power is that 1000W/L, temperature are under 65 ℃, mixture to step B carried out microwave treatment 3 minutes, after reaction finishes, centrifugation is fuel-displaced, water, solid phase, and wherein: separate the 0.5kg oil phase that obtains and be walnut oil, the 0.35kg water is the protein peptide aqueous solution, be walnut protein peptide, the 0.65kg solid phase is a residue.
The oil phase that present embodiment obtains is limpid walnut oil, and water then boils the enzyme that went out in 3 minutes through 90 ℃ to be handled, and lyophilize gets walnut protein peptide meal 0.2kg again, solid phase residue drying, pulverize dietary fiber food 0.3kg.
Embodiment 2
A. the hulled walnut kernel mill is walnut mud;
B. get the walnut mud of 1kg steps A, and add the compound protease of forming by the papain of the Traumanase of 500,000 units and 500,000 units therein, add deionized water 1kg again and mix;
C. under the agitation condition of 120rpm, at microwave power is that 600W/L, temperature are under 45 ℃, mixture to step B carried out microwave treatment 10 minutes, after reaction finishes, centrifugation is fuel-displaced, water, solid phase, and wherein: the 0.52kg oil phase is walnut oil, and the 0.83kg water is the protein peptide aqueous solution, be walnut protein peptide, the 0.65kg solid phase is a residue.
The oil phase that present embodiment obtains is limpid walnut oil, and water is then handled through 90 ℃ of heating enzyme that went out in 5 minutes, and spraying drying gets walnut protein peptide meal 0.22kg again, solid phase residue drying, pulverize dietary fiber food 0.26kg.
Embodiment 3
A. de-oiling walnut meal mill is not walnut mud;
B. get the walnut mud of 1kg steps A, and add the compound protease of forming by 10,000 unit stomach en-s and 500,000 unit Traumanases therein, add distilled water 20kg again and mix;
C. under the agitation condition of 300rpm, at microwave power is that 10W/L, temperature are under 35 ℃, mixture to step B carried out microwave treatment 1 minute, after reaction finishes, centrifugation is fuel-displaced, water, solid phase, and wherein: the 0.54kg oil phase is walnut oil, and the 19.8kg water is the protein peptide aqueous solution, be walnut protein peptide, the 0.66kg solid phase is a residue.
The oil phase that present embodiment obtains is limpid walnut oil, water then boils the enzyme that went out in 3 minutes through 90 ℃ to be handled, adopting film filter to dam to obtain molecular weight again is component solution 0.45kg between 200～8000 dalton, greater than 8000 daltonian component 0.45kg, get smart powder 0.09kg of walnut small molecular protein peptide and the smart powder 0.06kg of walnut high molecular weight protein peptide through lyophilize respectively, all the other get instant walnut powder 0.1kg through concentrating with lyophilize; Solid phase residue drying, pulverize dietary fiber food 0.21kg.
Claims (9)
1. a microwave-assisted protease hydrolysis prepares the method for walnut oil and walnut protein peptide synchronously, it is characterized in that through the following step:
A. walnut raw material mill is walnut mud;
B. in the walnut mud of per kilogram steps A, add proteolytic enzyme or compound protease 10～1,000,000 units, add entry and mix by walnut mud ︰ water=mass ratio of 1 ︰ 0.5～20;
C. under the agitation condition of 60～300rpm, and microwave power is that 10～1000W/L, temperature are under 35～65 ℃, mixture to step B carried out microwave treatment 1～10 minute, after reaction finishes, centrifugation is fuel-displaced, water, solid phase, wherein: oil phase is walnut oil, and water is walnut protein peptide, and solid phase is a residue.
2. method according to claim 1 is characterized in that: the walnut raw material of described steps A is hulled walnut kernel or de-oiling walnut meal not.
3. method according to claim 1 is characterized in that: the water among the described step B is pure water, deionized water or distilled water.
4. method according to claim 1, it is characterized in that: the proteolytic enzyme among the described step B is the biological peptide bond hydrolysis enzyme of animal, plant, microorganism, comprise papoid, Traumanase, stomach en-, trypsinase, perhaps derive from the pronase e of microorganism, one or more the mixing in the bacillus proteolytic enzyme.
5. method according to claim 1, it is characterized in that: the compound protease among the described step B is papoid, Traumanase, stomach en-, trypsinase, perhaps derives from the pronase e of microorganism, one or more the mixing in the bacillus proteolytic enzyme.
6. method according to claim 1 is characterized in that: the residue of gained among the described step C, make dietary fiber food through conventional drying, pulverizing.
7. method according to claim 1 is characterized in that: the walnut oil of gained among the described step C, and directly edible or further through the stock oil of conventional low-temperature physics refining as table oil, health care oil, mixed oil.
8. method according to claim 1 is characterized in that: the walnut protein peptide of gained among the described step C, handle through the routine enzyme that goes out, and be directly used in and produce degreasing walnut protein peptide drink; Or prepare walnut antioxidant peptide active polypeptide products through conventional ultrafiltration, be used for healthcare products, foodstuff additive, perhaps nutrition-fortifying agent, makeup, cosmetics of everyday use; Or get the walnut protein peptide powder through conventional low temperature spray drying.
9. method according to claim 8 is characterized in that: the described enzyme that goes out is handled and is adopted conventional heating means to come inactivated proteases, and the condition that the enzyme that goes out is handled is 90 ℃ of heating 5 minutes or boiled 3 minutes.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010585253.1A CN102080107A (en) | 2010-12-13 | 2010-12-13 | Method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis |
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| CN201010585253.1A CN102080107A (en) | 2010-12-13 | 2010-12-13 | Method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103289809A (en) * | 2013-05-30 | 2013-09-11 | 西北师范大学 | Method for preparing walnut seed kernel oil by adopting brine enzymatic method |
| CN103932286A (en) * | 2014-04-01 | 2014-07-23 | 沈阳师范大学 | Walnut antioxidant polypeptide chewing flake and preparation method thereof |
| CN104222317A (en) * | 2014-08-21 | 2014-12-24 | 岳西县光圣茶油有限公司 | Stomach-nourishing millet tea oil |
| CN104222323A (en) * | 2014-08-21 | 2014-12-24 | 岳西县光圣茶油有限公司 | Spicy tea oil |
| CN104222324A (en) * | 2014-08-21 | 2014-12-24 | 岳西县光圣茶油有限公司 | Vine pepper wind-expelling and cold-removing tea oil |
| CN105777926A (en) * | 2016-03-22 | 2016-07-20 | 天津大学 | Method for comprehensively extracting effective components from walnuts |
| CN108796015A (en) * | 2018-06-13 | 2018-11-13 | 中国热带农业科学院南亚热带作物研究所 | A method of synchronizing extraction protein peptides and vegetable fat from Queensland nut |
| CN110616105A (en) * | 2019-09-18 | 2019-12-27 | 西昌市金粮山粮油食品有限公司 | Processing method of walnut oil |
| CN112795611A (en) * | 2021-01-25 | 2021-05-14 | 昆明生物制造研究院有限公司 | Method for preparing walnut protein polypeptide from insoluble protein |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101962596A (en) * | 2010-09-17 | 2011-02-02 | 昆明理工大学 | Method for synchronously preparing walnut oil and walnut peptide |
-
2010
- 2010-12-13 CN CN201010585253.1A patent/CN102080107A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101962596A (en) * | 2010-09-17 | 2011-02-02 | 昆明理工大学 | Method for synchronously preparing walnut oil and walnut peptide |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103289809B (en) * | 2013-05-30 | 2014-07-23 | 西北师范大学 | Method for preparing walnut seed kernel oil by adopting brine enzymatic method |
| CN103289809A (en) * | 2013-05-30 | 2013-09-11 | 西北师范大学 | Method for preparing walnut seed kernel oil by adopting brine enzymatic method |
| CN103932286B (en) * | 2014-04-01 | 2016-02-10 | 沈阳师范大学 | A kind of walnut antioxidation polypeptide chewable tablets and preparation method thereof |
| CN103932286A (en) * | 2014-04-01 | 2014-07-23 | 沈阳师范大学 | Walnut antioxidant polypeptide chewing flake and preparation method thereof |
| CN104222317A (en) * | 2014-08-21 | 2014-12-24 | 岳西县光圣茶油有限公司 | Stomach-nourishing millet tea oil |
| CN104222324A (en) * | 2014-08-21 | 2014-12-24 | 岳西县光圣茶油有限公司 | Vine pepper wind-expelling and cold-removing tea oil |
| CN104222323A (en) * | 2014-08-21 | 2014-12-24 | 岳西县光圣茶油有限公司 | Spicy tea oil |
| CN105777926A (en) * | 2016-03-22 | 2016-07-20 | 天津大学 | Method for comprehensively extracting effective components from walnuts |
| CN105777926B (en) * | 2016-03-22 | 2018-06-15 | 天津大学 | Method for comprehensively extracting effective components from walnuts |
| CN108796015A (en) * | 2018-06-13 | 2018-11-13 | 中国热带农业科学院南亚热带作物研究所 | A method of synchronizing extraction protein peptides and vegetable fat from Queensland nut |
| CN110616105A (en) * | 2019-09-18 | 2019-12-27 | 西昌市金粮山粮油食品有限公司 | Processing method of walnut oil |
| CN112795611A (en) * | 2021-01-25 | 2021-05-14 | 昆明生物制造研究院有限公司 | Method for preparing walnut protein polypeptide from insoluble protein |
| CN112795611B (en) * | 2021-01-25 | 2023-04-28 | 昆明生物制造研究院有限公司 | Method for preparing walnut protein polypeptide from insoluble protein |
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Application publication date: 20110601 |