CN103289809A - Method for preparing walnut seed kernel oil by adopting brine enzymatic method - Google Patents
Method for preparing walnut seed kernel oil by adopting brine enzymatic method Download PDFInfo
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- CN103289809A CN103289809A CN2013102085122A CN201310208512A CN103289809A CN 103289809 A CN103289809 A CN 103289809A CN 2013102085122 A CN2013102085122 A CN 2013102085122A CN 201310208512 A CN201310208512 A CN 201310208512A CN 103289809 A CN103289809 A CN 103289809A
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Abstract
The invention provides a method for preparing walnut seed kernel oil by adopting a brine enzymatic method. The method comprises the following steps of: firstly, drying walnut seed kernel at 40-60 DEG C, grinding the dried walnut seed kernel into pulp by a pulping machine, adding sodium chloride, alpha-amylase, cellulose and distilled water into the pulp, and evenly stirring and mixing; carrying out enzymolysis for 2-4h at 50-60 DEG C by stirring to obtain an enzymoiysis product; finally, carrying out centrifugation on the enzymoiysis product to obtain a centrifugal product which is the pure natural walnut seed kernel oil. After being analyzed by a gas chromatography-mass spectrometer (GC-MS), the walnut seed kernel oil prepared by the method contains rich unsaturated fatty acid, is high in oil quality, and has rich nutritive value, thus being high-quality edible oil and having functions of medical treatment and health care; furthermore, the enzymoiysis is carried out by compounding enzyme and the sodium chloride, so that the walnut seed kernel oil is low in dosage of enzyme, low in extraction cost and high in extraction ratio (79-82%) of oil product.
Description
Technical field
The present invention relates to a kind of preparation method of walnut seed kernel oil, relate in particular to the method that a kind of salt aqueous enzymatic method prepares the walnut seed kernel oil, belong to the vegetables oil processing technique field.
Background technology
Walnut (Juglans) belongs to Angiospermae Dicotyledoneae Juglandaceae (Juglanadaceae), cries English walnut again, originates in areas such as the European southeast, West Asia, is one of ancient fruit tree kind that China's cultivation history is long, germ plasm resource is abundant.Walnut is important nut and woody oil tree species, have very high economic worth: the walnut kernel percent is up to about 50%, and it is nutritious and delicious, can eat raw, also be the high quality raw material of making cake, be listed as the world's four big dry fruits with almond (California, USA almond), cashew nut, ear of maize.Walnut has fine health-care effect simultaneously, and China people claim that it is " long live ", " long-lived fruit ", and person is abroad arranged, and it is " strong man's food " or " nutritious nut ".Often eat that walnut can not only nourish blood vessels, improves a poor appetite, pitch-black beard and hair, and can also cure sexual hypofunction, weak, amnesia etc., so among the people have saying of " often eating walnut, recover one's youthful vigour ".The walnut kernel oleaginousness reaches 60% ~ 70%, and wherein unsaturated fatty acid content is more than 90%, and is also higher than the oleaginousness of soybean, Semen Brassicae campestris, peanut and sesame, have the title of " woody oleiferous plants king ".In addition, also contain compositions such as linolic acid that human body can not synthesize and linolenic acid, oleic acid in the walnut oil, and multiple VITAMIN and mineral element to the human body beneficial, as flavonoid, VE and iron etc., therefore, walnut oil is nutritious, and delicious delicate fragrance also, the nutritive health-care and the pharmacological effect that have kept walnut kernel, so walnut oil is a kind of nutritional oil of preciousness.
CN200910011719.4 provides a kind of preparation method of walnut oil, is through squeezing, filtration, winterization, process such as squeezing and getting repeatedly with walnut kernel.Because the consumption of squeezing required drive is big, to equipment requirement of strength height, extraction yield is lower.CN201010579440.9 provides a kind of working method of walnut oil, be with walnut kernel through squeezing, atomizing decon, come unstuck, decolouring, deodorization, filtration, obtain walnut oil.Though this method obtains the walnut oil of higher quality, complex technical process, cost is higher.CN201210561556.9 provides the technology of a kind of water for the legal system walnut oil, is with the baking of stoning peach kernel, fragmentation.Mill sauce, convert slurry, calmly technologies such as slurry, oil skimming are finished.The subject matter that this method exists is that extraction yield is low, and power consumption is big, during baking the quality of oil product is caused very big influence.
Summary of the invention
The objective of the invention is at problems of the prior art, provide a kind of salt aqueous enzymatic method to prepare the method for walnut seed kernel oil.
Salt aqueous enzymatic method of the present invention prepares the method for walnut seed kernel oil, be with walnut kind benevolence and 40-60 ℃ of drying, after paste roller mill is worn into slip, add sodium-chlor, α-Dian Fenmei, cellulase and distilled water in its slurry, making the solid-liquid ratio of mixed system is 1:4 ~ 1:6g/mL, mixes; Under 50 ℃ ~ 60 ℃, stir enzymolysis 2 ~ 4h, get enzymolysis product; At last with enzymolysis product in centrifugal, centrifugal product namely gets pure natural walnut seed kernel oil.
The add-on of described sodium-chlor is 8% ~ 12% of walnut kind benevolence quality; The addition of the vigor of α-Dian Fenmei 〉=3700 unit of activity/g, α-Dian Fenmei is 0.6% ~ 1% of walnut kind benevolence quality; The vigor of cellulase is 10000u/g, and its addition is 8% ~ 12% of walnut kind benevolence quality.
Described centrifugal be centrifugal 15 ~ 30min under the rotating speed of temperature, 6000 ~ 8000r/min at 15 ~ 25 ℃.
After measured, the inventive method prepares the extraction rate reached of walnut seed kernel oil 79% ~ 82%.
The walnut seed kernel oil of the present invention preparation utilizes in the walnut seed kernel oil of GC-MS lipid acid and content to analyze:
Esterification method: adopt the ester-interchange method among the GB/T17376-2008;
GC conditions: GC condition: RTX-5MS type elastic quartz capillary tube (30 m * 0.25 mm * 0.25 μ m); Carrier gas He, flow velocity 1.0mL/min; Splitting ratio is 50:1.The transmission line temperature is 250 ℃; 250 ℃ of injector temperatures.Temperature programming: initial temperature keeps 3min for 160 ℃; Be elevated to 210 ℃ with 2 ℃/min from 160 ℃, keep 1 min; Be elevated to 250 ℃ with 5 ℃/min from 210 ℃, keep 1 min, sample size is 1.0 μ L;
Mass spectrum condition: 250 ℃ of ion source temperatures, electron energy 70 eV, sweep limit 50 ~ 650 amu.
Gas-chromatography is seen Fig. 1.Lipid acid and content see Table 1 in the walnut seed kernel oil that GC-MS measures.The result of Fig. 1 and table 1 shows that unsaturated fatty acids reaches more than 98% in the gained walnut oil of the present invention, and nutritive ingredient is very abundant.
Fatty acid component and the content of table 1 gained walnut oil of the present invention
In sum, the following beneficial effect of the relative prior art of the present invention:
1, contain abundant unsaturated fatty acids in the Semen Juglandis oil of the present invention's preparation, the oil quality height has extremely abundant nutritive value, is the edible oil of high-quality, has medical treatment and health-care effect simultaneously;
2, the present invention adopts the composite enzymolysis that carries out of enzyme and sodium-chlor, and the consumption of enzyme is few, and extraction cost is low, the extraction yield height of oil product.
3, to prepare the technology of Semen Juglandis oil simple in the present invention, and power consumption is little, environment-protecting clean, but industrialization production.
Description of drawings
Fig. 1 is gained walnut of the present invention oil composition and mol ratio gas-chromatography.
Embodiment
Preparation method to walnut kernel of the present invention is described further below by specific embodiment.
Embodiment 1
(1) takes by weighing the walnut kind benevolence (oleaginousness is 60% ~ 70%) of 20g, after drying about 40 ℃, wear into slip through the sesame oil paste roller mill;
(2) sodium-chlor of adding 1.6g in slip, (vigor 〉=3700 unit of activity/g), 2.0g cellulase (vigor 10000u/g) adds the 100mL adding distil water to the 0.16g α-Dian Fenmei then, mixes;
(3) mixed system is stirred enzymolysis 2h down at 50 ℃, get enzymolysis product;
(4) with enzymolysis product centrifugal 15min under 15 ℃ of temperature, rotating speed 6000r/min; Collect the centrifugal product in upper strata, be pure natural walnut seed kernel oil, extraction yield is 78.93%.
Embodiment 2
(1) takes by weighing the walnut kind benevolence (oleaginousness is 60% ~ 70%) of 20g, after drying about 50 ℃, wear into slip through the sesame oil paste roller mill;
(2) sodium-chlor of adding 2.0g in slip, (vigor 〉=3700 unit of activity/g), 2.4g cellulase (vigor 10000u/g) adds the 120mL adding distil water to the 0.2g α-Dian Fenmei then, mixes;
(3) mixed system is stirred enzymolysis 3h down at 55 ℃, get enzymolysis product;
(4) with enzymolysis product centrifugal 20min under 20 ℃ of temperature, rotating speed 7000r/min; Collect the centrifugal product in upper strata, be pure natural walnut seed kernel oil, extraction yield is 80.35%.
Embodiment 3
(1) takes by weighing the walnut kind benevolence (oleaginousness is 60% ~ 70%) of 20g, after drying about 60 ℃, wear into slip through the sesame oil paste roller mill;
(2) sodium-chlor of adding 2.4g in slip, (vigor 〉=3700 unit of activity/g), 2.8g cellulase (vigor 10000u/g) adds 140 mL adding distil waters to the 0.24g α-Dian Fenmei then, mixes;
(3) mixed system is stirred enzymolysis 4h down at 60 ℃, get enzymolysis product;
(4) with enzymolysis product centrifugal 30min under 25 ℃ of temperature, rotating speed 8000r/min; Collect the centrifugal product in upper strata, be pure natural walnut seed kernel oil, extraction yield is 79.06%.
Claims (6)
1. the salt aqueous enzymatic method prepares the method for walnut seed kernel oil, be earlier with walnut kind benevolence and 40-60 ℃ of drying, after paste roller mill is worn into slip to wherein adding sodium-chlor, α-Dian Fenmei, cellulase and distilled water, mix; Under 50 ℃～60 ℃, stir enzymolysis 2～4h then, get enzymolysis product; At last with enzymolysis product in centrifugal, centrifugal product namely gets pure natural walnut seed kernel oil.
2. the salt aqueous enzymatic method prepares the method for walnut seed kernel oil according to claim 1, and it is characterized in that: the add-on of described sodium-chlor is 8%～12% of walnut kind benevolence quality.
3. the salt aqueous enzymatic method prepares the method for walnut seed kernel oil according to claim 1, and it is characterized in that: the vigor of described α-Dian Fenmei 〉=3700 unit of activity/g, the addition of α-Dian Fenmei are 0.6%～1% of walnut kind benevolence quality.
4. the salt aqueous enzymatic method prepares the method for walnut seed kernel oil according to claim 1, and it is characterized in that: the vigor of described cellulase is 10000u/g, and its addition is 8%～12% of walnut kind benevolence quality.
5. the salt aqueous enzymatic method prepares the method for walnut seed kernel oil according to claim 1, and it is characterized in that: the solid-liquid ratio of mixed system is 1:4～1:6g/mL.
6. the salt aqueous enzymatic method prepares the method for walnut seed kernel oil according to claim 1, it is characterized in that: described centrifugal be centrifugal 15～30min under the rotating speed of temperature, 6000～8000r/min at 15～25 ℃.
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Cited By (3)
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| CN104450152A (en) * | 2013-09-23 | 2015-03-25 | 贵州周以晴生物科技有限公司 | Compound technology for extracting tea seed oil employing aqueous enzymatic method |
| CN105565404A (en) * | 2015-12-25 | 2016-05-11 | 浙江农林大学 | Pecan oil aqueous enzymatic extraction method |
| CN108998213A (en) * | 2018-06-15 | 2018-12-14 | 临江市山花绿色食品有限公司 | A kind of preparation method of walnut oil |
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Cited By (4)
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| CN104450152A (en) * | 2013-09-23 | 2015-03-25 | 贵州周以晴生物科技有限公司 | Compound technology for extracting tea seed oil employing aqueous enzymatic method |
| CN104450152B (en) * | 2013-09-23 | 2017-11-21 | 贵州周以晴生物科技有限公司 | A kind of combination process of extracting tea seed oil by using enzymatic hydrolysis |
| CN105565404A (en) * | 2015-12-25 | 2016-05-11 | 浙江农林大学 | Pecan oil aqueous enzymatic extraction method |
| CN108998213A (en) * | 2018-06-15 | 2018-12-14 | 临江市山花绿色食品有限公司 | A kind of preparation method of walnut oil |
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Effective date of registration: 20180604 Address after: 043700 the new town of Yuanqu County, Shanxi Province, Zuo Jia Wan Village Patentee after: Yuanqu County Shun Tu original flavor agricultural and sideline products development Co., Ltd. Address before: 730070 No. 967 Ning Ning Road, Anning District, Lanzhou, Gansu Patentee before: Northwest Normal University |