CN103013648B - Method for preparing vegetable seed kernel oil and reclaiming protein through assistance of biological enzyme - Google Patents
Method for preparing vegetable seed kernel oil and reclaiming protein through assistance of biological enzyme Download PDFInfo
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Abstract
The invention relates to a method for preparing vegetable seed kernel oil and reclaiming protein through assistance of biological enzyme. The method comprises the following steps: 1) preparing and preprocessing vegetable seed kernels; 2) crushing and tempering the vegetable seed kernels; 3) freezing and unfreezing the vegetable seed kernels; 4) grinding the vegetable seed kernels into thick liquid and performing enzymolysis and digestion on the vegetable seed kernels, wherein the step can be realized in that hot water is added in unfrozen squeezed vegetable seed kernel powder for grinding the thick liquid in a colloid mill to obtain squeezed vegetable seed kernel thick liquid, and the mass of the hot water is 2-6 times of that of the vegetable seed kernel powder; the squeezed vegetable seed kernel thick liquid is passed in an ultrasonic reaction kettle which is provided with a mechanical stirring device; the pH value of the squeezed vegetable seed kernel thick liquid is adjusted within 8.0-10.0 by using NaOH solution; after alkaline protease is added, mechanical stirring and an ultrasonic generator are started to carry out the enzymolysis reaction; the total reaction time is not less than 1 hour; the mass of the alkaline protease is 0.5-1.5 percent of that of the squeezed vegetable seed kernel powder; and then the vegetable seed kernel thick liquid is obtained; and 5) separating oil and reclaiming the protein. The method has high oil yield.
Description
Technical field
The present invention relates to the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, belong to the applied technical field of biological enzyme technology in grease processing.
Background technology
In oil prodution industry, the auxiliary grease extractive technique of enzyme process is certainly a kind of emerging technology.It is compared and has many good qualities with traditional extracting method, such as, the enzyme process auxiliary process does not have the consumption of solvent substantially, and the investment cost is lower, and energy requirement is also less.In addition, the auxiliary grease extraction process of enzyme process can be from oilseeds refiltered oil and albumen simultaneously, the oil quality of output is good, meets the requirement of national standard; This technique is extracted the oil obtained not to be needed further to come unstuck, and some toxin and anti-nutrition component in oilseeds also are removed in the course of processing.In a sense, some are such as saving cost, and the demands such as protection of the environment, safety in production and enhancing nutrition have triggered the innovation of grease extractive technique.By the development enzyme process, assist oil extracting process successful Application in production, just can well meet these demands.
Within nearest five times more than ten years, extract oil technology with aqua method from oilseeds and launch some researchs.Although this concept has potential magnetism, but the oil extraction technique with traditional organic solvent based on normal hexane, ether and No. 6 solvents etc. is compared, the grease yield of aqua method oil extracting process is lower, the residual oil content that in filter residue (is sometimes in protein isolate) is high, and this has hindered the commercial applications of aqua method grease extractive technique.Some researchs show, the enzyme that has hydrolytic action by use can solve the low problem of aqua method grease extraction yield.Thereby can helping oil to discharge, these enzymes increase the grease yield from oilseeds.In addition, environmental problem, the particularly growing care to the volatile organic compounds by the generation of machine solvent, make the auxiliary oil extracting process of enzyme process become the focus that people pay close attention to.
Start late to the research of the auxiliary liquefaction of enzyme process in domestic aspect, 2004, the Liu Zhiqiang philosophy adopted prozyme (proteolytic enzyme+cellulase+polygalacturonase), proteolytic enzyme, cellulase, polygalacturonase and hemicellulase to carry out aqueous enzymatic method to rapeseed decorticate and gets oil.Result of study shows, different enzymes affect different on oil and albumen yield, and prozyme is the highest, and the proteolytic enzyme effect is more obvious, and cellulase takes second place, and the three is all higher than extracting without the enzyme aqua.Domestic patent aspect, mainly disclose at present the oil plants such as tea seed (ZL200910039039.3), peanut (ZL 200810021343.0), maize germ (ZL200410014630.0), sunflower seeds (CN1952094A) and sesame (CN101224022A) are adopted to enzyme process assisted Extraction oil and albumen, the patented technology that yet there are no vegetable seed or vegetable seed benevolence (desquamated rapeseed) enzyme process assisted Extraction oil and albumen is open.From above-mentioned disclosed technology, ubiquity grease yield is large than low, water consumption, add that the enzyme class is more, enzymolysis time is long, need constantly to regulate the pH value with the requirement that adapts to different process and the problem such as energy consumption is higher.
Summary of the invention
Problem to be solved by this invention is to provide a kind of biological enzyme the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen for the deficiency of above-mentioned prior art existence, and the method grease yield is high.
The technical scheme that the problem that the present invention is the above-mentioned proposition of solution adopts is as follows:
The auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, is characterized in that it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: the vegetable seed raw material is dropped in peeler and shells and obtain vegetable seed benevolence with separation of hull and kernel, and in its vegetable seed benevolence, skin-containing rate is controlled between 2～8wt%; Then vegetable seed benevolence is heated to 90～110 ℃ and keep 5～15min with open steam;
2) fragmentation of vegetable seed benevolence and modified: vegetable seed benevolence is put in double spiral squeezer, carried out at normal temperatures fragmentation and pre-pressing, obtain vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake; The vegetable seed benevolence squeeze cake obtained is pulverized, made it pass through 10～60 mesh standard sieves, obtained squeezing vegetable seed benevolence powder; Add water in squeezing vegetable seed benevolence powder, make its average moisture adjust to 20～50wt%;
3) vegetable seed benevolence freezing with thaw: by moisture, be that 20～50wt% squeezing vegetable seed benevolence powder is put into freezing plant that temperature is-4～-18 ℃ and carried out after freezing 2～6h taking out thawing;
4) vegetable seed benevolence defibrination and Enzymolysis: in the squeezing vegetable seed benevolence powder thawed, add the hot water (hot water temperature is 45～85 ℃) of 2～6 times of its quality to carry out defibrination in colloidal mill, obtain squeezing vegetable seed benevolence slurry;
Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution (concentration is 0.5～1mol/L) and adjust to 8.0～10.0, and add squeezing vegetable seed benevolence powder (to be by 10～60 mesh standard sieves, the squeezing vegetable seed benevolence powder obtained) after the Sumizyme MP of quality 0.5～1.5%, open mechanical stirring and ultrasonic generator and carry out enzyme digestion reaction, total reaction time is not less than 1h, obtains vegetable seed benevolence slurries;
5) oil separating and albumen reclaim: after enzyme digestion reaction completes, vegetable seed benevolence slurries are sent in whizzer and carry out oily water separation and solid-liquid separation, obtain vegetable seed benevolence edible vegetable oil (being vegetable seed benevolence oil), emulsion layer, protein solution and slag; Protein solution obtains vegetable seed aqueous soluble protein or polypeptide (being the albumen of required recovery) after the concentrated spray drying;
Emulsion layer is skimmed with slag blanket (being slag) and merges, after again adding water and carry out enzymolysis by the method in step 4), centrifugation again, so repeat 1～5 time, until residual oil content [being the ratio of oleaginousness and the oleaginousness in raw material vegetable seed benevolence powder (being by 10～60 mesh standard sieves the squeezing vegetable seed benevolence powder obtained) of slag] is not more than 5wt% relatively; Merge separating the vegetable seed benevolence edible vegetable oil obtained after each enzymolysis, obtain vegetable seed benevolence oil after vacuum-drying; To after the slag vacuum-drying finally obtained, obtain the desquamated rapeseed dregs of rice;
Step 2) the vegetable seed benevolence normal temperature squeezing crude oil obtained in is filtered through essence, obtains vegetable seed benevolence normal temperature pressed oil (being vegetable seed benevolence oil) after deodorizing.
Press such scheme, step 1) described by vegetable seed benevolence with after open steam heating, make its moisture adjust to 6～18wt%.
Press such scheme, step 2) the described employing double spiral squeezer Residual oil content that squeezes at normal temperatures the vegetable seed benevolence squeeze cake obtained is 10～26wt%.
Press such scheme, step 4) described in the squeezing vegetable seed benevolence powder thawed added hot water temperature be 45～85 ℃.
Press such scheme, step 4) the enzyme digestion reaction temperature of described squeezing vegetable seed benevolence slurry in the ultrasonic response still is 45～75 ℃, mechanical stirring speed is 120～300r/min, ultrasonic frequency is 20～98KHz, each Ultrasonic Radiation interval time is 5～60min, and each Ultrasonic Radiation time length is 10～30min.
Press such scheme, described emulsion layer is skimmed and slag blanket merges, and then after adding water and carrying out enzymolysis, then centrifugation, the multiplicity of whole process is 1～5 time.
The present invention is according to the structure compositing characteristic of vegetable seed benevolence, first utilize the broken vegetable seed benevolence of physics mode, adopt again the enzyme preparation degrades vegetable seed benevolence filtered out, and combine freezing and ultrasonic technology co-treatment, thereby promote the release of grease in vegetable seed benevolence and increase protein hydrolysis degree, further improving the grease yield.
Beneficial effect of the present invention is:
1) adopt desquamated rapeseed as raw material, and adopt wet heat treatment before the enzymolysis fragmentation, make the abundant passivation of endogenous enzyme energy and inactivation in vegetable seed, avoided the generation of some antinutritional factor, improved the quality of oil and albumen.
2) not being that squeeze vegetable seed benevolence fuel-displaced is purpose as far as possible, is purpose but take suitable broken vegetable seed benevolence, adopts double spiral squeezer to be processed vegetable seed benevolence, can suitably turn down the gun pressure power of pressing, and increases the output of unit time, and energy consumption and mechanical wear are reduced.Compare all right high-quality grease of recovery part simultaneously with the breaking method (as toothed roll crusher) that adopts other, reduce the pressure that the back enzymolysis is put forward oily operation.
3) thus adopting slow freezing to make in broken vegetable seed benevolence cell to form larger ice crystal is fully destroyed vegetable seed benevolence cellularstructure, contribute to the enzymolysis of proteolytic enzyme, grease can fully be discharged; Adopt ultrasonic wave to carry out assistance enzymolysis, can strengthen Pignus pignoris efficiency, reduce the emulsification degree of oil in water, be conducive to oily water separation and reduce enzyme processing time, improve the oil recovery rate.
4) after enzymolysis, by after the slurries centrifugation, emulsion layer and slag blanket are added to water more again and carry out enzymolysis, reduced the inhibition of product to enzyme digestion reaction, make enzyme digestion reaction more abundant, grease is obtained through refining more thorough.
Embodiment
In order to understand better the present invention, further illustrate content of the present invention below in conjunction with embodiment, within it is included in the scope of protection of the invention, but do not limit the present invention.
Embodiment 1
The auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: the vegetable seed after vibratory screening apparatus, stone remover, magnetic separator, selection by winnowing is dropped into to peeler and shelled, regulate the peeler parameter, make shelling obtain skin-containing rate in vegetable seed benevolence by separation of hull and kernel and be controlled at 6wt%.Then vegetable seed benevolence is transported in modified storehouse and heats (vapor temperature is 105 ℃) and keep 10min with open steam, make vegetable seed benevolence moisture adjust to 18wt%.
2) fragmentation of vegetable seed benevolence and modified: the vegetable seed benevolence after moisture is adjusted is transported to double spiral squeezer from modified storehouse, carries out at normal temperatures fragmentation and pre-pressing, obtains vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake (Residual oil is 19wt%).Vegetable seed benevolence normal temperature squeezing crude oil is transported in fine filting machine and is filtered, and then sends into vacuum-drying in vacuum deodorizing tower and obtains vegetable seed benevolence pressed oil (being vegetable seed benevolence oil); Vegetable seed benevolence squeeze cake enters vibratory screening apparatus and is sieved (cross 30 mesh standard sieves) and obtain squeezing vegetable seed benevolence powder after sending in pulverizer and being pulverized.
3) after squeezing vegetable seed benevolence powder and being transported in cool chamber and adding suitable quantity of water to make its average moisture adjust to 40wt%, regulate after the cool chamber temperature carries out freezing 6h to-12 ℃, the cool chamber temperature is adjusted to room temperature (30 ℃) and thawed.
4) after thawing, by squeezing vegetable seed benevolence powder, adding 60 ℃ of left and right hot water of 5 times of its quality and be transported in colloidal mill, carry out defibrination, obtain squeezing vegetable seed benevolence slurry.
Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution (concentration is 1mol/L) and adjust to 9.5, and add squeezing vegetable seed benevolence powder (to be by 30 mesh standard sieves, the squeezing vegetable seed benevolence powder obtained) Sumizyme MP (Novozymes Company of quality 1.5%, Alcalase 2.4L), (ultrasonic frequency is 40KHz to open mechanical stirring (stirring velocity is 200r/min) and ultrasonic generator, each Ultrasonic Radiation interval time is 10min, each Ultrasonic Radiation time length is 15min) carry out enzyme digestion reaction, hydrolysis temperature is controlled at 60 ℃, total reaction time is 1.5h.
5) after enzymolysis completes, vegetable seed benevolence slurries are sent in disk centrifugal separator and carry out oily water separation and solid-liquid separation, obtain vegetable seed benevolence edible vegetable oil, emulsion layer, protein solution and slag; After protein solution is sent in vacuum decker and concentrates, then be transported in spray-dryer and carry out spraying drying and obtain vegetable seed aqueous soluble protein or polypeptide;
The emulsion layer of separation is skimmed after merging with slag blanket after again adding water and is transported to in the ultrasonic response still of mechanical stirring device, by same operational condition and step, carrying out enzymolysis, centrifugation, repeat operation 2 times.The vegetable seed benevolence edible vegetable oil merging that separation after each enzymolysis is obtained is sent into vacuum-drying in vacuum deodorizing tower and is obtained vegetable seed benevolence oil; The slag finally obtained is sent in vacuum drying oven and obtained the desquamated rapeseed dregs of rice (residual oil content is 5wt% relatively) after dehydration.[grease obtained (vegetable seed benevolence pressed oil, vegetable seed benevolence oil) quality and raw material vegetable seed benevolence powder (are by 30 mesh standard sieves the grease yield, the squeezing vegetable seed benevolence powder obtained) ratio of grease total mass in] be 90%, the oil obtained does not need further refining just can reach national 3 grade standards, and the gained rape seed protein can replace soybean protein to use.
Embodiment 2
The auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: the vegetable seed after vibratory screening apparatus, stone remover, magnetic separator, selection by winnowing is dropped into to peeler and shelled, regulate the peeler parameter, make shelling obtain skin-containing rate in vegetable seed benevolence by separation of hull and kernel and be controlled at the 5wt% left and right.Then vegetable seed benevolence is transported in modified storehouse and heats (vapor temperature is 108 ℃) and keep 8min with open steam, make vegetable seed benevolence moisture adjust to the 16wt% left and right.
2) fragmentation of vegetable seed benevolence and modified: the vegetable seed benevolence after moisture is adjusted is transported to double spiral squeezer from modified storehouse, carries out at normal temperatures fragmentation and pre-pressing, obtains vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake (Residual oil is 18wt%).Vegetable seed benevolence normal temperature squeezing crude oil is transported in fine filting machine and is filtered, and then sends into vacuum-drying in vacuum deodorizing tower and obtains vegetable seed benevolence pressed oil; Vegetable seed benevolence squeeze cake enters vibratory screening apparatus and is sieved (cross 20 mesh standard sieves) and obtain squeezing vegetable seed benevolence powder after sending in pulverizer and being pulverized.
3) after squeezing vegetable seed benevolence powder and being transported in cool chamber and adding suitable quantity of water to make its average moisture adjust to 45wt%, regulate after the cool chamber temperature carries out freezing 5h to-15 ℃, the cool chamber temperature is adjusted to room temperature (30 ℃ of left and right) and thawed.
4) after thawing, by squeezing vegetable seed benevolence powder, adding 70 ℃ of left and right hot water of 4.5 times of its quality and be transported in colloidal mill, carry out defibrination, obtain squeezing vegetable seed benevolence slurry.
Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution (concentration is 0.8mol/L) and adjust to 9.0, and add the Sumizyme MP of squeezing vegetable seed benevolence opaque amount 1.2%, (ultrasonic frequency is 45KHz to open mechanical stirring (stirring velocity is 260r/min) and ultrasonic generator, each Ultrasonic Radiation interval time is 20min, each Ultrasonic Radiation time length is 10min) carry out enzyme digestion reaction, hydrolysis temperature is controlled at 70 ℃, and total reaction time is 2.0h.
5) after enzymolysis completes, vegetable seed benevolence slurries are sent in disk centrifugal separator and carry out oily water separation and solid-liquid separation, obtain vegetable seed benevolence edible vegetable oil, emulsion layer, protein solution and slag; After protein solution is sent in vacuum decker and concentrates, then be transported in spray-dryer and carry out spraying drying and obtain vegetable seed aqueous soluble protein or polypeptide; Skim after merging with slag blanket after again adding water and be transported to in the ultrasonic response still of mechanical stirring device, by same operational condition and step, carrying out enzymolysis, centrifugation separating emulsion layer, repeat operation 3 times.The vegetable seed benevolence edible vegetable oil merging that separation after each enzymolysis is obtained is sent into vacuum-drying in vacuum deodorizing tower and is obtained vegetable seed benevolence oil; The slag finally obtained is sent in vacuum drying oven and obtained the desquamated rapeseed dregs of rice (residual oil content is 4wt% relatively) after dehydration.Grease yield (ratio of grease total mass in the oil quality obtained and raw material vegetable seed benevolence powder) is 92%, and the oil obtained does not need further refining just can reach national 3 grade standards, and the gained rape seed protein can replace soybean protein to use.
Embodiment 3
The auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: the vegetable seed after vibratory screening apparatus, stone remover, magnetic separator, selection by winnowing is dropped into to peeler and shelled, regulate the peeler parameter, make shelling obtain skin-containing rate in vegetable seed benevolence by separation of hull and kernel and be controlled at the 4wt% left and right.Then vegetable seed benevolence is transported in modified storehouse and heats (vapor temperature is 108 ℃) and keep 9min with open steam, make vegetable seed benevolence moisture adjust to the 17wt% left and right.
2) fragmentation of vegetable seed benevolence and modified: the vegetable seed benevolence after moisture is adjusted is transported to double spiral squeezer from modified storehouse, carries out at normal temperatures fragmentation and pre-pressing, obtains vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake (Residual oil is 17wt%).Vegetable seed benevolence normal temperature squeezing crude oil is transported in fine filting machine and is filtered, and then sends into vacuum-drying in vacuum deodorizing tower and obtains vegetable seed benevolence pressed oil; Vegetable seed benevolence squeeze cake enters vibratory screening apparatus and is sieved (cross 40 mesh standard sieves) and obtain squeezing vegetable seed benevolence powder after sending in pulverizer and being pulverized.
3) after squeezing vegetable seed benevolence powder and being transported in cool chamber and adding suitable quantity of water to make its average moisture adjust to 48%, regulate after the cool chamber temperature carries out freezing 4h to-17 ℃, the cool chamber temperature is adjusted to room temperature (30 ℃) and thawed.
4) after thawing, by squeezing vegetable seed benevolence powder, adding 65 ℃ of left and right hot water of 4 times of its quality and be transported in colloidal mill, carry out defibrination, obtain squeezing vegetable seed benevolence slurry.
Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution (concentration is 0.6mol/L) and adjust to 8.5, and add the Sumizyme MP of squeezing vegetable seed benevolence opaque amount 1.0%, (ultrasonic frequency is 25KHz to open mechanical stirring (stirring velocity is 300r/min) and ultrasonic generator, each Ultrasonic Radiation interval time is 18min, each Ultrasonic Radiation time length is 12min) carry out enzyme digestion reaction, hydrolysis temperature is controlled at 65 ℃, and total reaction time is 3.0h.
5) after enzymolysis completes, vegetable seed benevolence slurries are sent in disk centrifugal separator and carry out oily water separation and solid-liquid separation, obtain vegetable seed benevolence edible vegetable oil, emulsion layer, protein solution and slag; After protein solution is sent in vacuum decker and concentrates, then be transported in spray-dryer and carry out spraying drying and obtain vegetable seed aqueous soluble protein or polypeptide; Skim after merging with slag blanket after again adding water and be transported to in the ultrasonic response still of mechanical stirring device, by same operational condition and step, carrying out enzymolysis, centrifugation separating emulsion layer, repeat operation 1 time.The vegetable seed benevolence edible vegetable oil merging that separation after each enzymolysis is obtained is sent into vacuum-drying in vacuum deodorizing tower and is obtained vegetable seed benevolence oil; The slag finally obtained is sent in vacuum drying oven and obtained the desquamated rapeseed dregs of rice (residual oil content is 4wt% relatively) after dehydration.Grease yield (ratio of grease total mass in the oil quality obtained and raw material vegetable seed benevolence powder) is 91%, and the oil obtained does not need further refining just can reach national 3 grade standards, and the gained rape seed protein can replace soybean protein to use.
Embodiment 4
The auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: the vegetable seed after vibratory screening apparatus, stone remover, magnetic separator, selection by winnowing is dropped into to peeler and shelled, regulate the peeler parameter, make shelling obtain skin-containing rate in vegetable seed benevolence by separation of hull and kernel and be controlled at 2wt%.Then vegetable seed benevolence is transported in modified storehouse and heats (vapor temperature is 110 ℃) and keep 5min with open steam, make vegetable seed benevolence moisture adjust to 6wt%.
2) fragmentation of vegetable seed benevolence and modified: the vegetable seed benevolence after moisture is adjusted is transported to double spiral squeezer from modified storehouse, carries out at normal temperatures fragmentation and pre-pressing, obtains vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake (Residual oil is 10wt%).Vegetable seed benevolence normal temperature squeezing crude oil is transported in fine filting machine and is filtered, and then sends into vacuum-drying in vacuum deodorizing tower and obtains vegetable seed benevolence pressed oil; Vegetable seed benevolence squeeze cake enters vibratory screening apparatus and is sieved (cross 10 mesh standard sieves) and obtain squeezing vegetable seed benevolence powder after sending in pulverizer and being pulverized.
3) after squeezing vegetable seed benevolence powder and being transported in cool chamber and adding suitable quantity of water to make its average moisture adjust to 20wt%, regulate after the cool chamber temperature carries out freezing 2h to-4 ℃, the cool chamber temperature is adjusted to room temperature (25 ℃) and thawed.
4) after thawing, by squeezing vegetable seed benevolence powder, adding 45 ℃ of hot water of 2 times of its quality and be transported in colloidal mill, carry out defibrination, obtain squeezing vegetable seed benevolence slurry.
Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution (concentration is 0.5mol/L) and adjust to 8.0, and add the Sumizyme MP (the Properase 1600L of Genencor company) of squeezing vegetable seed benevolence opaque amount 0.5%, (ultrasonic frequency is 20KHz to open mechanical stirring (stirring velocity is 120r/min) and ultrasonic generator, each Ultrasonic Radiation interval time is 5min, each Ultrasonic Radiation time length is 10min) carry out enzyme digestion reaction, hydrolysis temperature is controlled at 45 ℃, total reaction time is 1.0h.
5) after enzymolysis completes, vegetable seed benevolence slurries are sent in disk centrifugal separator and carry out oily water separation and solid-liquid separation, obtain vegetable seed benevolence edible vegetable oil, emulsion layer, protein solution and slag; After protein solution is sent in vacuum decker and concentrates, then be transported in spray-dryer and carry out spraying drying and obtain vegetable seed aqueous soluble protein or polypeptide;
The emulsion layer of separation is skimmed after merging with slag blanket after again adding water and is transported to in the ultrasonic response still of mechanical stirring device, by same operational condition and step, carrying out enzymolysis, centrifugation, repeat operation 1 time.The vegetable seed benevolence edible vegetable oil merging that separation after each enzymolysis is obtained is sent into vacuum-drying in vacuum deodorizing tower and is obtained vegetable seed benevolence oil; The slag finally obtained is sent in vacuum drying oven and obtained the desquamated rapeseed dregs of rice (residual oil content is 5wt% relatively) after dehydration.Grease yield (ratio of grease total mass in the oil quality obtained and raw material vegetable seed benevolence powder) is 90%, and the oil obtained does not need further refining just can reach national 3 grade standards, and the gained rape seed protein can replace soybean protein to use.
Embodiment 5
The auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: the vegetable seed after vibratory screening apparatus, stone remover, magnetic separator, selection by winnowing is dropped into to peeler and shelled, regulate the peeler parameter, make shelling obtain skin-containing rate in vegetable seed benevolence by separation of hull and kernel and be controlled at 8wt%.Then vegetable seed benevolence is transported in modified storehouse and heats (vapor temperature is 103 ℃) and keep 15min with open steam, make vegetable seed benevolence moisture adjust to 18wt%.
2) fragmentation of vegetable seed benevolence and modified: the vegetable seed benevolence after moisture is adjusted is transported to double spiral squeezer from modified storehouse, carries out at normal temperatures fragmentation and pre-pressing, obtains vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake (Residual oil is 26wt%).Vegetable seed benevolence normal temperature squeezing crude oil is transported in fine filting machine and is filtered, and then sends into vacuum-drying in vacuum deodorizing tower and obtains vegetable seed benevolence pressed oil; Vegetable seed benevolence squeeze cake enters vibratory screening apparatus and is sieved (cross 60 mesh standard sieves) and obtain squeezing vegetable seed benevolence powder after sending in pulverizer and being pulverized.
3) after squeezing vegetable seed benevolence powder and being transported in cool chamber and adding suitable quantity of water to make its average moisture adjust to 50wt%, regulate after the cool chamber temperature carries out freezing 6h to-18 ℃, the cool chamber temperature is adjusted to room temperature (20 ℃) and thawed.
4) after thawing, by squeezing vegetable seed benevolence powder, adding 85 ℃ of hot water of 6 times of its quality and be transported in colloidal mill, carry out defibrination, obtain squeezing vegetable seed benevolence slurry.
Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution (concentration is 1mol/L) and adjust to 10.0, and add the Sumizyme MP (Novozymes Company of squeezing vegetable seed benevolence opaque amount 1.5%, Alcalase 2.4L), (ultrasonic frequency is 98KHz to open mechanical stirring (stirring velocity is 300r/min) and ultrasonic generator, each Ultrasonic Radiation interval time is 60min, each Ultrasonic Radiation time length is 30min) carry out enzyme digestion reaction, hydrolysis temperature is controlled at 75 ℃, total reaction time is 1.5h.
5) after enzymolysis completes, vegetable seed benevolence slurries are sent in disk centrifugal separator and carry out oily water separation and solid-liquid separation, obtain vegetable seed benevolence edible vegetable oil, emulsion layer, protein solution and slag; After protein solution is sent in vacuum decker and concentrates, then be transported in spray-dryer and carry out spraying drying and obtain vegetable seed aqueous soluble protein or polypeptide;
The emulsion layer of separation is skimmed after merging with slag blanket after again adding water and is transported to in the ultrasonic response still of mechanical stirring device, by same operational condition and step, carrying out enzymolysis, centrifugation, repeat operation 5 times.The vegetable seed benevolence edible vegetable oil merging that separation after each enzymolysis is obtained is sent into vacuum-drying in vacuum deodorizing tower and is obtained vegetable seed benevolence oil; The slag finally obtained is sent in vacuum drying oven and obtained the desquamated rapeseed dregs of rice (residual oil content is 3wt% relatively) after dehydration.Grease yield (ratio of grease total mass in the oil quality obtained and raw material vegetable seed benevolence powder) is 92%, and the oil obtained does not need further refining just can reach national 3 grade standards, and the gained rape seed protein can replace soybean protein to use.
Claims (4)
1. the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, is characterized in that it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: the vegetable seed raw material is dropped in peeler and shells and obtain vegetable seed benevolence with separation of hull and kernel, and in its vegetable seed benevolence, skin-containing rate is controlled between 2～8wt%; Then vegetable seed benevolence heated with open steam and keep 5～15min; Described by vegetable seed benevolence with after open steam heating, make its moisture adjust to 6～18wt%;
2) fragmentation of vegetable seed benevolence and modified: vegetable seed benevolence is put in double spiral squeezer, carried out at normal temperatures fragmentation and pre-pressing,
Obtain vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake; The vegetable seed benevolence squeeze cake obtained is pulverized, made it pass through 10～60 mesh standard sieves, obtained squeezing vegetable seed benevolence powder; Add water in squeezing vegetable seed benevolence powder, make its average moisture adjust to 20～50wt%;
3) vegetable seed benevolence freezing with thaw: by moisture, be that 20～50wt% squeezing vegetable seed benevolence powder is put into freezing plant that temperature is-4～-18 ℃ and carried out after freezing 2～6h taking out thawing;
4) vegetable seed benevolence defibrination and Enzymolysis: in the squeezing vegetable seed benevolence powder thawed, add the hot water of 2～6 times of its quality to carry out defibrination in colloidal mill, obtain squeezing vegetable seed benevolence slurry; Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution and adjust to 8.0～10.0, and after adding the Sumizyme MP of squeezing vegetable seed benevolence opaque amount 0.5～1.5%, open mechanical stirring and ultrasonic generator and carry out enzyme digestion reaction, total reaction time is not less than 1h, obtains vegetable seed benevolence slurries;
Described in the squeezing vegetable seed benevolence powder thawed added hot water temperature be 45～85 ℃;
The enzyme digestion reaction temperature of described squeezing vegetable seed benevolence slurry in the ultrasonic response still is 45～75 ℃, mechanical stirring speed is 120～300r/min, ultrasonic frequency is 20～98KHz, and each Ultrasonic Radiation interval time is 5～60min, and each Ultrasonic Radiation time length is 10～30min;
5) oil separating and albumen reclaim: after enzyme digestion reaction completes, vegetable seed benevolence slurries are sent in whizzer and carry out oily water separation and solid-liquid separation, obtain vegetable seed benevolence edible vegetable oil, emulsion layer, protein solution and slag; Protein solution obtains vegetable seed aqueous soluble protein or polypeptide after the concentrated spray drying;
Emulsion layer is skimmed and slag blanket merges, after again adding water and carry out enzymolysis by the method in step 4), then centrifugation, so repeat 1～5 time, until residual oil content is not more than 5wt% relatively; Merge separating the vegetable seed benevolence edible vegetable oil obtained after each enzymolysis, obtain vegetable seed benevolence oil after vacuum-drying; To after the slag vacuum-drying finally obtained, obtain the desquamated rapeseed dregs of rice;
Step 2) the vegetable seed benevolence normal temperature squeezing crude oil obtained in is filtered through essence, obtains vegetable seed benevolence normal temperature pressed oil after deodorizing.
2. the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme according to claim 1, is characterized in that step 2) the described employing double spiral squeezer Residual oil content that squeezes at normal temperatures the vegetable seed benevolence squeeze cake obtained is 10～26wt%.
3. the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme according to claim 1, is characterized in that step 4) concentration of described NaOH solution is 0.5～1mol/L.
4. the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme according to claim 1, is characterized in that step 1) temperature of described open steam heating is 90～110 ℃.
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| CN105296164A (en) * | 2015-12-02 | 2016-02-03 | 无限极（中国）有限公司 | Extraction method of cinnamon oil |
| CN105462681A (en) * | 2015-12-23 | 2016-04-06 | 刘家容 | Extracting process regulating grease ratio of colza oil |
| CN105713718A (en) * | 2016-01-25 | 2016-06-29 | 蚌埠市江淮粮油有限公司 | Method using millet bran to synchronously extract millet bran oil and millet bran protein |
| CN105713717A (en) * | 2016-01-25 | 2016-06-29 | 蚌埠市江淮粮油有限公司 | Method for synchronous extraction of rice bran oil and vegetable proteins |
| CN105602706A (en) * | 2016-01-25 | 2016-05-25 | 蚌埠市江淮粮油有限公司 | Method for synchronously extracting rice bran oil and rice bran protein from rice bran |
| CN107325884A (en) * | 2016-04-29 | 2017-11-07 | 湖南农业大学 | A kind of method for extracting chia seed grease |
| CN108004009A (en) * | 2017-11-30 | 2018-05-08 | 潍坊友容实业有限公司 | A kind of Suaeda salsa seed oil and albumen integrated extraction technique |
| CN110564495A (en) * | 2019-09-30 | 2019-12-13 | 湖南新金浩茶油股份有限公司 | Tea oil with strong flavor and preparation method thereof |
| CN112812882B (en) * | 2020-12-24 | 2021-08-31 | 青岛吴昊植物油有限公司 | Production process of fried embryo water-extracted peanut oil |
| CN112574276B (en) * | 2020-12-31 | 2021-08-03 | 潍坊希普生物科技有限公司 | Extraction process of rapeseed protein |
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