WO2012079219A1

WO2012079219A1 - Methods for preparing shrimp grease and preparing shrimp concentrate or shrimp med from euphausia superba

Info

Publication number: WO2012079219A1
Application number: PCT/CN2010/079749
Authority: WO
Inventors: 朱蓓薇; 周大勇; 秦磊; 李冬梅; 董秀萍; 吴海涛; 杨静峰; 孙黎明; 迟雅丽
Original assignee: 大连工业大学
Priority date: 2010-12-14
Filing date: 2010-12-14
Publication date: 2012-06-21
Also published as: JP2013523100A; JP5357362B2

Abstract

Methods for preparing the shrimp grease and preparing the shrimp concentrate or the shrimp med from the Euphausia superba include the following steps: homogenizing or crushing the Euphausia superba to obtain the krill raw material; autolyzing the krill raw material at 0-4℃ after ultraviolet irradiation to get the solution; treating the autolyzed solution by ultrasonic; adjusting the PH value of the solution and realizing the enzymolysis by adding exogenous enzymes; and extracting the krill grease, or obtaining the shrimp concentrate after condensing the filtered solution of the enzymolysis solution or the shrimp med after spray drying the filtered solution. The methods have the following advantages: simple operation, high efficiency, and the trophicity and functionality of the product by maximally saving some original biologically active substances.

Description

Method for preparing shrimp oil and preparing shrimp concentrate or shrimp powder from Antarctic krill

The present invention relates to a method for extracting products based on Antarctic krill, and more particularly to a method for extracting shrimp oil or shrimp concentrate, shrimp powder.

Background technique

Antarctic krill (Euphausia superba) is one of the largest single biological resources on the planet. Its estimated stock is about 400-1.5 billion tons. The annual yield of mature shrimp is 300-500 million tons. About 100 million tons, forming huge potential fishery resources. In recent years, with the gradual depletion of the world's traditional fishery resources and the proposal of the 200-mile exclusive economic zone, the huge Antarctic krill resources in the Antarctic waters have attracted the attention of some developed countries. China has also included Antarctic krill resources as one of the major developmental species for future offshore fisheries development.

At present, the development of Antarctic krill resources is mainly based on the use of its protein, and the development and utilization of Antarctic krill oil is not sufficient. Antarctic krill lives in frigid waters, and shrimp oil is rich in phospholipids, which can account for more than 40% of total oil. Antarctic krill phospholipids are rich in omega-3 polyunsaturated fatty acids represented by DHA and EPA. Studies have shown that omega-3 polyunsaturated fatty acids have a wide range of beneficial health effects, including improving the prognosis of heart disease patients, increasing fetal growth rate, inhibiting tumor growth and metastasis, anti-inflammatory, anti-platelet aggregation, antihypertensive and Hyperlipidemia and regulation of the body's sensitivity to insulin. Japanese scholars point out that omega-3 polyunsaturated fatty acids in the form of phospholipids have an advantage in cardiovascular activity. This indicates that the nutritional and medicinal value of phospholipids under Antarctic Phobia is very high, and it has a good prospect for development and utilization.

Antarctica is located in the distant, Antarctic krill after long-term transportation and preservation. Because Antarctic krill is rich in polyunsaturated fatty acids, it is prone to spoilage. Therefore, Antarctic krill fishing needs to be quickly frozen and stored at low temperature to ensure the original quality of phospholipids, which greatly increases the transportation and preservation cost of Antarctic krill, and becomes a bottleneck restricting the development and utilization of Antarctic krill phospholipid resources. To solve the problem of transportation and preservation of Antarctic krill, the best way is to process Antarctic krill quickly after harvesting to prepare phospholipids. However, there is still a lack of on-board processing and preparation methods for Antarctic krill products.

Summary of the invention

The present invention is directed to the above problems, and aims to provide a method for preparing an Antarctic krill beneficial substance after the Antarctic krill fishing, which is particularly suitable for direct operation on a fishing vessel.

In order to achieve the above object, the present invention first provides a basic method for preparing shrimp oil based on Antarctic krill, comprising the following steps:

Step l Raw material processing: Drain the harvested Antarctic krill and homogenate or crush to obtain the krill raw material; Step 2 Autolysis: After irradiating the krill raw material with ultraviolet rays, it is autolyzed at 0~4 °C. Obtained from the solution; Step 3 ultrasonic treatment: the ultrasonic solution is used to treat the self-solution after adding water; Step 4 exogenous enzyme enzymatic hydrolysis: adjust the pH of the self-solution to 7.5 ~ 8.0, add alkaline protease to the self-solution, enzymatic hydrolysis at 55 ~ 65 ° C, to obtain an enzymatic hydrolysate;

Step 5 extracts krill oil by using ethanol or n-ethane based on the enzymatic hydrolysate.

In a preferred embodiment, the alkaline protease is a Bacillus subtilis alkaline protease. The ultrasonic wave used in Step 3 has a power of 300 to 800 watts and a wavelength of 200 to 400 nanometers, and the self-solution time is 10 to 30 minutes. The ultraviolet light used in Step 2 has a power of 20 to 40 watts, a wavelength of 200 to 300 nm, an irradiation distance of 0.5 to 1 m, and a treatment time of 10 to 35 minutes; and the autolysis time after ultraviolet treatment is 2 to 10 hours. Adjust the pH value in the above steps, and choose HC1 or NaOH with a concentration of 1~6M. According to the above basic method, a preferred method for preparing shrimp oil according to the present invention comprises the following steps: Step 1 : Antarctic krill homogenate to obtain a homogenate;

Step 2. After irradiating the homogenate with ultraviolet rays for 25 to 35 minutes, autolysis is performed to obtain the self-solution; and then auto-dissolving at a high temperature (in order to increase product yield), which specifically includes the following four steps:

Step 2-1, adding 0.5 to 1 volume of water to the self-solution, and the final concentration is

2.0~2.5% sodium chloride solution and a final concentration of 0.05~0.1% ethanol;

Step 2-2, again use ultraviolet light with a power of 20~40 watts and a wavelength of 200~300 nm.

0.5-1 meter to irradiate the self-solution for 10 to 40 minutes;

Step 2-3, adjusting the pH of the self-solution to 7.5~8.0;

Step 2-4, heating the solution from a temperature of 3 to 8 ° C every 10 to 30 minutes to 30 - 65 ° C, and autolysis for 2 to 8 hours;

Step 3, adding 0.5 to 1.5 times the volume of water to the solution; using ultrasonic treatment,

Step 4, after adjusting the pH value, the alkaline protease of 9~10×10 ⁵ U/kg substrate is added to the self-solution, and the enzymatic hydrolysate is obtained by enzymatic hydrolysis for 1-4 hours;

Step 5, adding 0.5 to 1.5 volumes of n-hexane to the enzymatic hydrolysate, allowing to stand for 30 to 90 minutes, and centrifuging to obtain a first upper liquid phase and a first precipitate; after removing the n-hexane from the first upper liquid phase Get shrimp oil.

Preferably, in the above step, the improvement of the preparation of the shrimp oil is as follows: In Step 5, after obtaining the first precipitate, 0.4~0.6 times of water is added to the first precipitate, and the inlet air temperature in the drying tower is 140~ At 170 ° C, the exhaust gas temperature is 60-90 ° C for spray drying to obtain a dry powder, which is transported back to the land for use as a raw material for extracting nutrients. In Step 5, the method for removing n-hexane is as follows: The n-hexane is removed by concentration under reduced pressure at 30 to 45 ° C, and n-hexane is recovered to achieve the purpose of recycling.

In order to further increase the yield, the above scheme for preparing shrimp oil is further improved: in step 5, 0.5 to 1.5 times the volume of n-hexane is added to the first precipitate, allowed to stand for 20 to 60 minutes, and centrifuged to obtain a second upper liquid phase. And a second precipitate; after mixing the first upper liquid phase with the second upper liquid phase, removing n-hexane to obtain shrimp oil. The extraction rate of shrimp oil after high temperature autolysis and secondary precipitation operation can reach 88.79~94.56%. Therefore, the invention greatly improves the extraction efficiency of shrimp oil while reducing the operation cost, and saves The transportation cost of Antarctic krill also guarantees the quality of krill products, which is very suitable for on-site operation on fishing vessels and has high economic benefits.

In addition, 0.4~0.6 times of water is added to the second precipitate, and the drying temperature of the drying tower is 140-170 ° C, and the exhaust temperature is 60-90 ° C for spray drying to obtain a dry powder. Shipped back to land for use as a raw material for extracting nutrients. According to the above basic method, the present invention also provides a preferred method for preparing shrimp phospholipids, comprising the following steps:

Step 1 , Antarctic krill homogenate to obtain a homogenate;

Step 2. Irradiation of the homogenate with ultraviolet rays for 10 to 30 minutes after autolysis;

Step 3, adding 1 to 3 volumes of water to the solution, and treating with ultrasonic waves;

Step 4. After adjusting the pH, the alkaline proteinase of 9~10χ105 U/kg substrate is added to the solution, and the enzymatic hydrolysate is obtained by enzymatic hydrolysis at 55~65 °C for 1-4 hours;

Step 5, the enzymatic hydrolysate is spray-dried to obtain a dry powder; adding 5 to 15 volumes of 90% ethanol to the dry powder, mixing, standing for 30 to 90 minutes, and centrifuging to obtain a third upper liquid phase And a third precipitate; the third liquid phase removes ethanol to obtain an Antarctic krill phospholipid.

In a preferred embodiment, the above-mentioned improvement of the preparation of the insecticidal phospholipid is as follows: In order to further increase the yield, in Step 5, after obtaining the third precipitate, first adding 2.5 to 7.5 volumes of ethanol to the third precipitate, and mixing. After standing for 20 to 60 minutes, the mixture is centrifuged to obtain a fourth upper liquid phase and a fourth precipitate; the third upper liquid phase and the fourth upper liquid phase are mixed, and ethanol is removed to obtain an antarctic phospholipid lower phospholipid. The method for removing ethanol in Step 5 is as follows: The ethanol is concentrated under reduced pressure at 40 to 60 ° C, and ethanol is recovered.

Further, in the above-described scheme for preparing a bad phospholipid, Step 1 and Step 2 are operated on a fishing boat; and between Step 2 and Step 3, the homogenate is directly frozen on a fishing boat. Correspondingly, Step 3 first melts the frozen slurry and returns it to the slurry state, which can be operated on the shore or on board.

In addition, the above-mentioned scheme for preparing bad phospholipids, Step 1 and Step 2 are carried out on a fishing boat; and between Step 2 and Step 3, on the fishing boat, the slurry is spray-dried to obtain a dry powder. Correspondingly, Step 3 is operated on the shore or on board. First, the dry powder needs to be dissolved in 7 to 12 times of volume of water to prepare the state of the slurry. The invention also provides a method for preparing shrimp concentrate and shrimp powder based on Antarctic krill, comprising the following steps:

Step 1 Raw material processing: drain the harvested Antarctic krill and homogenate or crush to obtain the krill raw material; Step 2 autolysis: After irradiating the krill raw material with ultraviolet rays, it is autolyzed at 0~4 °C. Obtained from the solution; Step 3 ultrasonic treatment: the ultrasonic solution is used to treat the self-solution after adding water;

Step 4 exogenous enzyme enzymatic hydrolysis: adjust the pH of the self-solution to 7.5 ~ 8.5, add to the self-solution The low temperature protease of 9~10x l0 ⁵ U/kg substrate is enzymatically hydrolyzed at 10~20 °C for 1-4 hours to obtain enzymatic hydrolysate;

Step 5: The enzymatic hydrolysate is filtered through 120~200 mesh; the filtrate or concentrated in low temperature vacuum to obtain 40%~70% of Antarctic krill concentrate, or after spray drying Get krill dry powder.

In a preferred embodiment, the low temperature protease described in Step 4 is selected from Novozymes Low Temperature Protease produced by Novozymes (China) Investment Co., Ltd. The ultrasonic wave in Step 3 is 300-800 watts of power, the wavelength is 200-400 nm, and the self-solution is processed for 5-15 minutes. The ultraviolet light in Step 2 is 20 to 40 watts, the wavelength is 200 to 300 nm, and the distance is 0.5 to 1 meter. The treatment is for 10 to 30 minutes, and the autolysis is 1 to 5 hours after the ultraviolet treatment. Adjust the pH value and choose HC1 or NaOH at a concentration of 1~6M.

In a preferred mode, in step 5, or at a concentration temperature of 20 to 25 ° C, a vacuum of 75 kPa to 90 kPa, a low temperature vacuum concentration is performed to obtain a concentrate; or the drying tower inlet air temperature is 150 to 180 ° C, and the exhaust temperature is Spray drying was carried out at 75 to 95 ° C to obtain a dry powder.

Further, the purpose of the "drainage" of the present invention is to remove the water adhering to the fresh Antarctic krill, and the shrimp itself remains in a fresh shrimp state after being drained. The purpose of ultraviolet radiation is to enhance the autolysis ability of Antarctic krill; the purpose of ultrasonic treatment is to enhance the enzymatic ability.

The invention firstly applies the autolysis technology and the ultrasonic technology to the extraction method of the Antarctic krill grease, and extracts the process operation single, high efficiency, good product purity and quality, suitable for promotion, and high economic benefit. Due to the strong autolysis ability of the fresh Antarctic krill, the amount of exogenous enzyme is reduced and the production cost is reduced. Due to the use of ultrasonic treatment, the extraction rate of Antarctic krill oil is increased, and the benefit is increased. The method of the invention has mild conditions and can preserve some physiological active substances in the Antarctic krill oil to the maximum extent, so that the product has both nutrition and functionality. The invention has the following advantages:

1. The operating process cartridge of the present invention does not require complicated equipment and is suitable for carrying on a fishing boat.

2. The invention utilizes the strong autolysis ability of the Antarctic krill itself, which can reduce the amount of exogenous enzymes and reduce the production cost.

3. The present invention applies ultrasonic technology to the extraction of Antarctic krill oil for the first time, which greatly improves the yield of Antarctic phosphorus shrimp and improves economic benefits.

4. The temperature of the whole operation process of the invention is low, and the physiological active substances in the Antarctic krill grease can be preserved to the utmost while reducing the energy consumption.

5. The invention uses the low-temperature protease for the first time to prepare the Antarctic krill concentrate and powder, and the temperature of the whole operation process is low, and the physiological active substances in the Antarctic krill lipid can be preserved to the maximum while reducing the energy consumption. .

6. The present invention firstly uses crushing technology for the preparation of Antarctic krill concentrates and powders, which has a low degree of damage to the Antarctic krill shell and minimizes the release of fluorine from the shrimp shell.

detailed description

Example 1: An onboard processing method for preparing shrimp oil based on Antarctic krill comprises the following steps: 1. Raw materials and treatment: The freshly harvested Antarctic krill is drained and directly homogenized. 2. Low temperature autolysis: The Antarctic krill slurry is treated with ultraviolet light with a power of 20 to 40 watts and a wavelength of 200 to 300 nm for 0.5 to 1 meter for 25 to 35 minutes, and the treated slurry is placed at 0 to 4 degrees. C (average ambient temperature in the Antarctic region during the fishing season) is autolyzed for 2 to 10 hours.

3, high temperature autolysis: 0.5 to 1 volume of water, sodium chloride (final concentration of 2.0% to 2.5%) and ethanol (0.05% to 0.1% homogenate) were added to the slurry after the second step treatment. Volume), with a power of 20 ~ 40 watts, a wavelength of 200 ~ 300 nanometers of ultraviolet light distance of 0.5 ~ 1 m irradiation treatment for 10 to 40 minutes, with 1 ~ 6M of HC1 or NaOH, the pH of the slurry is adjusted to 7.5 ~ 8.0, the homogenate Increasing the temperature to 3~8 °C every 10~30 minutes to 30~65 °C, autolysis for 2~8 hours.

4. Ultrasound-assisted exogenous enzyme enzymatic hydrolysis: Add 0.5~1.5 times of water to the homogenate treated in the third step, mix well, and treat with 10~30 with 300~800 watt ultrasonic wave (wavelength 200~400 nm). Minute, adjust the pH to 7.5~8.0 with 1-6M HC1 or NaOH, add alkaline protease to the homogenate

(9~10 < 10 ⁵ U/kg substrate), mix and digest at 55~65 °C for 1~4 hours;

5. Extraction of shrimp oil: Add 0.5 to 1.5 times the volume of n-hexane to the enzymatic hydrolysate, mix and let stand for 30 to 90 minutes, and centrifuge with a sedimentation centrifuge to obtain the upper liquid phase A and the precipitate A; Add 0.5 to 1.5 times the volume of n-hexane in A, mix well, let stand for 20 to 60 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase B and precipitate B; mix liquid phase A and liquid phase B, after 30~ Concentrated under reduced pressure at 45 ° C to remove n-hexane to obtain shrimp oil, and the recovered n-hexane can be reused. Precipitate B, adding 0.4-0.6 times volume of water, spray drying at a drying tower temperature of 140-170 ° C, exhaust temperature of 60-90 ° C, to obtain dry powder, transported back to the land as an extract protein and Raw materials for other nutrients.

Example 2: An onboard processing method for preparing shrimp oil based on Antarctic krill, comprising the following steps: directly draining the freshly harvested Antarctic krill, and then homogenizing the homogenate by a power of 40 watts, a wavelength of 245 nm, and a distance 0.5 m of ultraviolet radiation treatment for 25 minutes, low temperature autolysis for 2 hours at the average ambient temperature in the Antarctic region during the fishing season; 0.5 times volume of water and NaCl were added to the homogenate after low temperature autolysis treatment (final concentration was 2.0 ~2.5%) and ethanol (final concentration 0.05~0.1%), mix, the homogenate is treated by ultraviolet light with a power of 20 watts, a wavelength of 299 nm and a distance of 0.5 m for 10 minutes, and the pH of the slurry is adjusted with 1 M of HC1 or NaOH. The value is 7.5 to 8.0, and then the temperature is raised to 30 ° C at a rate of 8 ° C every 30 minutes, and the temperature is autolyzed for 8 hours under these conditions.

Add 0.5 times volume of water to the slurry after high temperature autolysis treatment, mix, and treat with ultrasonic power (295 nm) for 10 minutes, and adjust the pH of the slurry to 7.5~8.0 with 1M HC1 or NaOH. Alkaline protease (9x l0 ⁵ U/kg substrate) was added to the homogenate, mixed, and digested at 55 ° C for 1 hour.

0.5 volume of n-hexane was added to the above hydrolyzate, mixed, allowed to stand for 30 minutes, and centrifuged by a sedimentation centrifuge to obtain an upper liquid phase A and a precipitate A; 0.5 volume of n-hexane was added to the precipitate A, Mix, let stand for 20 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase B and the precipitate B; mix the liquid phase A and the liquid phase B, and concentrate the concentrated hexane after removing the hexane at 35 ° C to recover the shrimp oil. Hexane can be heavy The above-mentioned precipitate A and precipitate B are mixed, 0.6 times volume of water is added, and then spray-dried under the condition that the inlet air temperature of the drying tower is 140-170 ° C and the exhaust temperature is 60-90 ° C. It is dried and shipped back to the land as a raw material for extracting protein and other nutrients.

Example 3: An onboard processing method for preparing shrimp oil based on Antarctic krill, comprising the following steps: directly draining the freshly harvested Antarctic krill, and then homogenizing the homogenate by a power of 40 watts, a wavelength of 230 nm, and a distance 1 meter of ultraviolet irradiation treatment for 25 minutes, autolysis at 0 to 4 ° C for 10 hours; to the homogenate after low temperature autolysis treatment, add 1 volume of water, NaCl (final concentration of 2.0%) and Ethanol (final concentration: 0.05%), mix, the homogenate is treated with ultraviolet light of 40 watts, wavelength 295 nm, and distance of 0.5 m for 30 minutes. The pH of the slurry is adjusted to 7.5-8.0 with 4M HCl or NaOH. Increasing the temperature to 30 ° C at a rate of 5 ° C every 10 minutes, and auto-dissolving for 2 hours at this temperature; adding 1.5 volumes of water to the homogenate, mixing, and passing ultrasonic waves of 500 watts (390 Nano) After 13 minutes of treatment, adjust the pH of the slurry to 7.5-8.0 with 4M HCl or NaOH, add alkaline protease (9x l0 ⁵ U/kg substrate) to the homogenate, mix, and enzyme at 55 °C. Solution for 2 hours;

Add 1 volume of n-hexane to the above hydrolysate, mix, let stand for 60 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase A and precipitate A; add 1 volume of n-hexane to the precipitate A, Mix and let stand for 20 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase B and the precipitate B; mix the liquid phase A and the liquid phase B, and concentrate the concentrated hexane at 38 ° C to remove the hexane to obtain the shrimp oil. The n-hexane can be reused; the above precipitate A and precipitate B are mixed, 0.6 times volume of water is added, and then spray-dried at a drying tower temperature of 145 ° C and an exhaust temperature of 65 ° C. The dried powder is produced and transported back to the land as a raw material for extracting proteins and other nutrients.

Example 4: An onboard processing method for preparing shrimp oil based on Antarctic krill comprises the following steps: directly draining the freshly harvested Antarctic krill, and then homogenizing the homogenate by a power of 27 watts, a wavelength of 245 nm, and a distance 0.5 m of ultraviolet irradiation treatment for 30 minutes, autolysis at 0 to 4 ° C for 6 hours; adding 1 volume of water to the homogenate after low temperature autolysis treatment, the final concentration of NaCK is 2.0 to 2.5%) And ethanol (final concentration of 0.05~0.1%), mix, the homogenate is treated with ultraviolet light of 40 watts, wavelength of 200 nanometers and distance of 0.5 meters for 10 minutes, and the pH of the slurry is adjusted to 7.5 with 1M of HC1 or NaOH. 8.0, and then raise the temperature to 30 ° C at a rate of 3 ° C every 10 minutes, and autolyze at high temperature for 2 hours under this condition;

Add 1 volume of water to the slurry after high temperature autolysis, mix well, treat with ultrasonic power of 300 watts (wavelength 389 nm) for 21 minutes, and adjust the pH of the slurry to 7.5 with 1M HCl or NaOH. 8.0, adding alkaline protease (9*10 ⁵ U/kg substrate) to the homogenate, mixing, and digesting at 65 ° C for 4 hours;

0.5 volume of n-hexane was added to the above hydrolyzate, mixed, allowed to stand for 30 minutes, and centrifuged by a sedimentation centrifuge to obtain an upper liquid phase A and a precipitate A; 0.5 volume of n-hexane was added to the precipitate A, Mix, let stand for 20 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase B and precipitate B; Phase A and liquid phase B, after concentration and concentration under reduced pressure at 40 ° C to remove n-hexane, shrimp oil is obtained, and the recovered n-hexane can be reused; the above precipitate A and precipitate B are mixed, 0.6 times volume of water is added, and then Spray drying was carried out in a drying tower with an inlet air temperature of 160 ° C and an exhaust gas temperature of 85 ° C to obtain a dry powder, which was transported back to the land as a raw material for extracting proteins and other nutrients.

Example 5: An onboard processing method for preparing shrimp oil based on Antarctic krill, comprising the following steps: directly draining freshly harvested Antarctic krill, and homogenizing the slurry by a power of 33 watts, a wavelength of 210 nm, and a distance 0.5 m of ultraviolet radiation treatment for 30 minutes, autolysis at low temperature for 2 hours at 0 to 4 ° C (average ambient temperature in the Antarctic region during the fishing season); 1 volume of water added to the slurry after low temperature autolysis treatment , NaCl (final concentration of 2.0~2.5%) and ethanol (final concentration of 0.05~0.1%), mix, the homogenate is treated with ultraviolet light of 40 watts, wavelength 267 nm, distance 0.5 m for 40 minutes, with 1M The pH of the slurry is adjusted to 7.5~8.0 by HC1 or NaOH, and then heated to 50 °C at a rate of 3 °C every 10 minutes, and autolysis at this temperature for 8 hours under these conditions;

Add 0.5 times volume of water to the autoclaved slurry, mix it, and treat it with ultrasonic power of 500 watts (380 nm) for 27 minutes, and adjust the pH of the slurry to 7.5-8.0 with 1M HCl or NaOH. Add alkaline protease (9x l0 ⁵ U/kg substrate) to the homogenate, mix and digest for 3 hours at 65 ° C; add 1.5 volumes of n-hexane to the above hydrolysate, mix, and let stand After 60 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase A and the precipitate A. Add 1.5 volumes of n-hexane to the precipitate A, mix, let stand for 60 minutes, and centrifuge with a sedimentation centrifuge to obtain the upper liquid phase. B and precipitate B; mixed liquid phase A and liquid phase B, concentrated under reduced pressure at 40 ° C to remove n-hexane to obtain shrimp oil, the recovered n-hexane can be reused; the above precipitate A and precipitate B are mixed, added 0.6 times The volume of water is then spray-dried at a drying tower temperature of 160 ° C and an exhaust temperature of 85 ° C to produce a dry powder which is transported back to the land as a raw material for extracting proteins and other nutrients.

Example 6: An onboard processing method for preparing shrimp oil based on Antarctic krill, comprising the following steps: directly draining the freshly harvested Antarctic krill, and then homogenizing the homogenate by a power of 34 watts, a wavelength of 230 nm, and a distance 0.5 m of ultraviolet radiation treatment for 30 minutes, autolysis at low temperature for 6 hours at 0 to 4 ° C (average ambient temperature in the Antarctic region during the fishing season); 0.5 volume of water was added to the slurry after low temperature autolysis treatment , NaCl (final concentration of 2.0~2.5%) and ethanol (final concentration of 0.05~0.1%), mix, the homogenate is treated with ultraviolet light of 40 watts, wavelength 208 nm, distance 0.5 m for 40 minutes, with 1M The pH of the slurry was adjusted to 7.5-8.0 by HC1 or NaOH, and then heated to 65 °C at a rate of 3 ° C every 10 minutes, and autolyzed at this temperature for 5 hours under these conditions.

Add 1 volume of water to the high temperature autolyzed homogenate, mix, and treat with ultrasonic power (367 nm) for 28 minutes, and homogenize the pH of the slurry to 7.5~8.0 with 1M HCl or NaOH. Alkaline protease ( ⁹ x 105 U/kg substrate) was added to the homogenate, mixed, and digested at 56 ° C for 4 hours.

Add 1 volume of n-hexane to the above hydrolysate, mix, let stand for 90 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase A and precipitate A; add 1 volume of n-hexane to the precipitate A, Mixed Evenly, let stand for 40 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase B and the precipitate B; mix the liquid phase A and the liquid phase B, and remove the n-hexane under a reduced pressure at 40 ° C to obtain the shrimp oil, and recover the recovered Hexane can be reused. The above precipitate A and precipitate B were mixed, 0.6 times by volume of water was added, and then spray-dried at a drying tower temperature of 160 ° C and an exhaust temperature of 85 ° C to obtain a dry powder, which was transported back to the land. As a raw material for extracting proteins and other nutrients.

Example 7: A method for preparing a bad phospholipid based on Antarctic krill, comprising the following steps:

1. Raw material processing: Drain the fresh live Antarctic krill and directly homogenize it;

2. Low-temperature autolysis: The Antarctic krill homogenate is treated with ultraviolet light of 40 watts, wavelength of 300 nm, and distance of 0.5 m for 10 to 30 minutes, and the treated homogenate is placed at 0 to 4 ° C (fishing season, South Pole) The average ambient temperature of the area is 2 to 10 hours from autolysis.

The autoclaved Antarctic krill homogenate was directly frozen. (The above operation steps are all completed on board. The advantage is that it utilizes the strong self-dissolving ability of fresh Antarctic krill, increases the self-dissolution of structural proteins, and increases the yield of phospholipids.)

Ultrasound-assisted exogenous enzymatic hydrolysis: For frozen Antarctic krill pulp, after the frozen Antarctic krill pulp is melted, add 1~3 times of water and treat it with 300~800 watt ultrasonic (389 nm). Minute, adjust the pH to 7.5~8.0 with 1M HCl or NaOH, add alkaline protease to the homogenate

(9~10x l0 ⁵ U/kg substrate), mix and digest at 60 ° C for 1 hour.

Extraction of Antarctic Krill Phospholipids: The samples were hydrolyzed by exogenous enzymes to obtain dry powders by spray drying. Add 5~15 times the volume of 95% ethanol to the Antarctic krill dry powder, mix and let stand for 30~90 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase A and precipitate A; add 2.5~7.5 to the precipitate A. A volume of ethanol, mixed, allowed to stand for 20 to 60 minutes, centrifuged with a sedimentation centrifuge to obtain the upper liquid phase B and precipitate B; mixed liquid phase A and liquid phase B, concentrated under reduced pressure at 40 ° C to remove ethanol, The Antarctic krill phospholipid is obtained, and the recovered ethanol can be reused.

Example 8: A method for preparing a bad phospholipid based on Antarctic krill, comprising the following steps:

1. Raw materials and treatment: Drain the fresh live Antarctic krill and directly homogenize it;

2. Low temperature autolysis: The Antarctic krill homogenate is treated with ultraviolet light of 40 watts, wavelength of 200~300 nm and distance of 0.5 m for 10~30 minutes, and the treated homogenate is placed at 0~4 °C. Dissolve for 2~10 hours.

The autoclaved Antarctic krill homogenate is spray-dried to obtain a dry powder. (The above steps are all completed on board. The advantage is that it utilizes the strong self-dissolving ability of fresh Antarctic krill, increases the self-dissolution of structural proteins, and increases the yield of phospholipids.)

Ultrasound-assisted exogenous enzymatic hydrolysis: Dissolve dry powder in 7~12 volumes of water, mix well, treat with 300~800 watts of ultrasonic wave (200-400 nm) for 10 minutes, adjust pH to 7.5~8.0 with 1M HCI or NaOH. Alkaline protease (9~10x10 ⁵ U/kg substrate) was added to the homogenate, mixed, and digested at 60 ° C for 1 hour. Extraction of Antarctic Krill Phospholipids: The samples were hydrolyzed by exogenous enzymes to obtain dry powders by spray drying. Add 5~15 times the volume of 95% ethanol to the Antarctic krill dry powder, mix and let stand for 30~90 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase A and precipitate A; add 2.5~7.5 to the precipitate A. A volume of ethanol, mixed, allowed to stand for 20 to 60 minutes, centrifuged with a sedimentation centrifuge to obtain the upper liquid phase B and precipitate B; mixed liquid phase A and liquid phase B, concentrated under reduced pressure at 40 ° C to remove ethanol, The Antarctic krill phospholipid is obtained, and the recovered ethanol can be reused.

Example 9: A method for preparing a bad phospholipid based on Antarctic krill, comprising the following steps:

2. Low-temperature autolysis: The Antarctic krill homogenate is treated with ultraviolet light of 40 watts, a wavelength of 205 nm and a distance of 0.5 m for 10 minutes, and the treated homogenate is autolyzed for 2 hours at the fishing environment temperature;

3. Ultrasound-assisted exogenous enzymatic hydrolysis: Add 1 volume of water to the slurry, treat it with 300 watts of ultrasonic wave (350 nm) for 10 minutes, adjust the pH to 7.5 with 1M of HCl or NaOH, and add alkaline to the homogenate. The protease (9 < 10 ⁵ U/kg) was mixed and digested at 60 ° C for 1 hour; the sample digested with the exogenous enzyme was spray-dried to obtain a dry powder.

4. Extraction of phospholipids from Antarctic phosphorus: Add 5 times volume of 95% ethanol to the dry powder, mix, let stand for 30 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase A and precipitate A; add to the precipitate A 2.5 times volume of ethanol, mix, let stand for 20 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase B and precipitate B; mix liquid phase A and liquid phase B, and concentrate to remove ethanol after decompression at 40 ° C, ie Get Antarctic krill phospholipids.

Example 10: A method for preparing shrimp phospholipid based on Antarctic krill, comprising the following steps:

2. Low temperature autolysis: The Antarctic krill homogenate is treated with ultraviolet light of 40 watts, a wavelength of 210 nm and a distance of 0.5 m for 20 minutes, and the treated homogenate is autolyzed at 0 to 4 ° C for 5 hours;

3. Ultrasound-assisted exogenous enzymatic hydrolysis: Add 1~3 times of water to the slurry, treat it with 300~800W ultrasonic wave (380nm) for 10 minutes, adjust the pH to 7.5~8.0 with 4M HC1 or NaOH, Alkaline protease (9×10 ⁵ U/kg substrate) was added to the homogenate, mixed, and enzymatically hydrolyzed at 56 ° C for 1 hour; the digested sample was spray-dried to obtain a dry powder.

4. Extraction of phospholipids from Antarctic phosphorus worm: Add 5-15 times volume of 95% ethanol to the dry powder, mix, let stand for 30-90 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase A and precipitate A; Phase A is concentrated under reduced pressure at 41 °C to remove ethanol, and the phospholipids of the Antarctic Phosphorus are obtained, and the recovered ethanol can be reused.

Example 11: A method for preparing a bad phospholipid based on Antarctic krill, comprising the following steps:

2. Low-temperature autolysis: The Antarctic krill homogenate is treated with ultraviolet light of 40 watts, a wavelength of 240 nm and a distance of 0.8 m for 10 to 30 minutes, and the treated homogenate is autolyzed at 0 to 4 ° C for 6 hours. ;

3. The autoclaved Antarctic krill homogenate is directly stored at -15 ~ -25 °C for storage. 4. Ultrasound-assisted exogenous enzymatic hydrolysis: After melting the frozen Antarctic krill homogenate, add 2 volumes of water, treat with 550 watts of ultrasonic wave (250 nm) for 10 minutes, and adjust the pH to 7.75 with 1 M HCl or NaOH. Alkaline protease (9.5×10 ⁵ U/kg substrate) was added to the homogenate, mixed, and digested at 60 ° C for 3 hours; the sample was hydrolyzed by exogenous enzyme to obtain a dry powder by spray drying.

5. Extraction of phospholipids from Antarctic phosphorus: Add 10 times volume of 95% ethanol to the Antarctic krill dry powder, mix, let stand for 60 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase A and precipitate A; Add 5 volumes of ethanol to A, mix well, let stand for 40 minutes, centrifuge with a sedimentation centrifuge to obtain upper liquid phase B and precipitate B; mix liquid phase A and liquid phase B, and remove at 48 ° C under reduced pressure. Ethanol, which is an antarctic phospholipid, can be reused.

In a preferred mode, the above steps 1 to 3 are completed on a fishing boat, and the advantage is that the fresh antarctic krill has the characteristics of strong autolysis ability, increases the self-dissolution of the structural protein, and increases the yield of the phospholipid. Subsequent steps can be carried out by transporting the frozen homogenate back to the shore or on board as needed.

Example 12: A method for preparing a bad phospholipid based on Antarctic krill, comprising the following steps:

2. Low temperature autolysis: The Antarctic krill homogenate is treated with ultraviolet light of 40 watts, 255 nm wavelength and 0.9 m distance for 10 to 30 minutes, and the treated homogenate is autolyzed for 2 to 10 hours at the fishing environment temperature. ;

3. The autoclaved Antarctic krill homogenate is directly stored at -15 ~ -25 °C for storage.

4. Ultrasound-assisted exogenous enzymatic hydrolysis: After melting the frozen Antarctic krill pulp, add 1~3 times of water, treat with 300~800W ultrasonic wave (400nm) for 10 minutes, use 1M HC1 or NaOH. Adjust the pH to 7.5~8.0, add alkaline protease (9~10x l0 ⁵ U/kg substrate) to the homogenate, mix and digest for 3 hours at 61 °C; digest the sample by exogenous enzyme Dry powder is obtained by spray drying.

5. Extraction of phospholipids from Antarctic phosphorus: Add 5 to 15 times the volume of 95% ethanol to the Antarctic krill dry powder, mix and let stand for 30 to 90 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase A and precipitate. A; liquid phase A is concentrated under reduced pressure at 52 ° C to remove ethanol, that is, Antarctic krill phospholipids, and the recovered ethanol can be reused.

In a preferred manner, the above steps 1-3 are completed on a fishing boat, and the advantage is that the self-dissolving ability of the fresh Antarctic krill is utilized, the self-dissolution of the structural protein is increased, and the phospholipid yield is increased. Subsequent steps can be carried out by transporting the frozen homogenate back to the shore or on board as needed.

Example 13: A method for preparing a bad phospholipid based on Antarctic krill, comprising the following steps:

2. Low temperature autolysis: The Antarctic krill homogenate is treated with ultraviolet light of 40 watts, wavelength of 235 nm and distance of 0.5 m for 25 minutes, and the treated slurry is autolyzed for 8.5 hours at the fishing environment temperature;

4. Ultrasound-assisted exogenous enzymatic hydrolysis: After melting the frozen Antarctic krill homogenate, add 2.5 volumes of water, treat with 650 watts of ultrasound (345 nm) for 10 minutes, adjust pH with 1M of HC1 or NaOH. To 7.9, alkaline protease (10 < 10 ⁵ U/kg substrate) was added to the homogenate, mixed, and enzymatically hydrolyzed at 62 ° C for 4 hours; the sample was hydrolyzed by exogenous enzyme to be spray-dried. Dry powder.

5. Extraction of phospholipids from Antarctic phosphorus: Add 12.5 times the volume of 95% ethanol to the Antarctic krill dry powder, mix, let stand for 75 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase A and precipitate A; Add 6.5 volumes of ethanol to A, mix, let stand for 45 minutes, centrifuge with a sedimentation centrifuge to obtain upper liquid phase B and precipitate B; mix liquid phase A and liquid phase B, and remove at 55 °C under reduced pressure. Ethanol, which is an Antarctic krill phospholipid, can be recycled.

Example 14: A method for preparing a bad phospholipid based on Antarctic krill, comprising the following steps:

2. Low temperature autolysis: The Antarctic krill homogenate is treated with ultraviolet light of 40 watts, a wavelength of 200 nm and a distance of 0.5 m for 25 minutes, and the treated homogenate is autolyzed for 8 hours at the fishing environment temperature;

3. The homogenate is spray dried to obtain a dry powder, which is stored at -15 ~ -25 °C.

4. Ultrasound-assisted exogenous enzymatic hydrolysis: Dissolve the Antarctic krill dry powder in 11 volumes of water, mix well, treat with 700 watts of ultrasonic wave (300 nm) for 10 minutes, adjust the pH to 7.9 with 1M of HC1 or NaOH, and mix well. the slurry was added alkaline protease (10x l0 ⁵ U / kg of substrate), mixed and digested at 60 ° C for 1 hour. The sample is hydrolyzed by an exogenous enzyme and spray-dried to obtain a dry powder.

5. Extraction of phospholipids from Antarctic phosphorus: Add 14 times volume of 95% ethanol to the Antarctic krill dry powder, mix, let stand for 85 minutes, centrifuge with a sedimentation centrifuge to obtain the upper liquid phase A and precipitate A; Add 7 times of volume of ethanol to A, mix well, let stand for 55 minutes, centrifuge with a sedimentation centrifuge to obtain upper liquid phase B and precipitate B; mix liquid phase A and liquid phase B, and remove at 40 ° C under reduced pressure. Ethanol, which is an antarctic phospholipid, can be reused.

In a preferred mode, the above steps 1 - 3 are completed on a fishing boat, and the advantage is that the fresh antarctic krill has the characteristics of strong autolysis ability, increases the self-dissolution of the structural protein, and increases the yield of the phospholipid. Subsequent steps can be carried out by transporting the frozen dry powder back to the shore or on board as needed.

Example 15: A method for rapid preparation of an Antarctic krill concentrate and powder on board, comprising the following steps:

1. Raw material processing: The fresh Antarctic krill that has been harvested is drained and crushed to obtain a broken product.

2, low temperature autolysis: use the power of 20 ~ 40 watts, the wavelength of 200 ~ 300 nm of ultraviolet light distance 0.5 ~ 1 m to irradiate the broken material for 10 ~ 30 minutes; then at 0 ~ 4 ° C (fishing area ambient temperature) autolysis 1 ~5 hours.

3. Ultrasound-assisted low-temperature exogenous enzymatic hydrolysis: Add 1-3 volumes of water to the crushed material, and treat it with 300-800 watts of ultrasonic wave (wavelength 200-400 nm) for 5-15 minutes, using 1M HC1 or NaOH. Adjusting the pH of the suspension suspension to 7.5~8.5, adding a low temperature protease of 9~10x10 ⁵ U/kg substrate to the homogenate, and enzymatically hydrolyzing for 1-4 hours at 10-20 °C Enzymatic hydrolysate. Low temperature protease Weixin wave low temperature protease.

4. The enzymatic hydrolysate is passed through a 120-200 mesh sieve, filtered or concentrated under low temperature vacuum to obtain an Antarctic krill concentrate, or spray dried to obtain an Antarctic krill dry powder.

Example 16: A rapid preparation method of antarctic krill concentrate and powder on board, the steps are as follows: The fresh Antarctic krill is drained and crushed, and the crushed material is irradiated with ultraviolet rays by 20 watts, a wavelength of 232 nm, and a distance of 0.5 m. After minute UV irradiation, it was autolyzed for 1 hour at the ambient temperature of the fishing area. After 1 volume of water in the low temperature autolyzed slurry and treated with 300 watts of ultrasonic wave (345 nm) for 5 minutes, the pH of the suspension suspension was adjusted to 7.5 with 1 M HCl or NaOH, A low temperature protease containing ⁹ x 105 U/kg of substrate was added to the homogenate for 1 hour at 10 °C. The hydrolyzate was passed through a sieve of 120 mesh, and the filtrate was concentrated under vacuum at a concentration of 20 to 25 ° C and a vacuum of 75 to 90 kPa to obtain a concentrate.

Example 17: A method for rapid preparation of an Antarctic krill concentrate and powder on board, the steps are as follows: The fresh Antarctic krill is drained and crushed, and the crushed material is passed through 20 watts, a wavelength of 245 nm, and a distance of 1 m of ultraviolet irradiation. The crushed material was autolyzed for 3 hours at ambient temperature in the fishing area after 10 minutes of ultraviolet irradiation. After treating the volume of water in a low-temperature autolyzed slurry with 1 volume of water and treating with 500 watts of ultrasonic wave (265 nm) for 10 minutes, the pH of the suspension suspension was adjusted to 8.0 with 1 M of HCl or NaOH. The low temperature protease to which 10 ⁶ U/kg of substrate was added to the homogenate was digested at 10 ° C for 3 hours. The hydrolyzate was passed through a sieve of 160 mesh, and the filtrate was concentrated under vacuum at a concentration of 25 ° C and a vacuum of 75 kPa to obtain a concentrate.

Example 18: A method for rapid preparation of an Antarctic krill concentrate and powder on board, the steps are as follows: The fresh Antarctic krill is drained and crushed, and the crushed material is irradiated with 20 watts, a wavelength of 255 nm, and a distance of 0.75 m. The crushed material was autolyzed for 5 hours at ambient temperature in the fishing area after 20 minutes of ultraviolet irradiation. After 3 times the volume of water in the autolyzed slurry at low temperature and treated with 500 watts of ultrasonic wave (270 nm) for 10 minutes, the pH of the suspension suspension was adjusted to 8.0 with 1 M HCl or NaOH, A low temperature protease containing ⁹ x 105 U/kg of substrate was added to the homogenate for 1 hour at 20 °C. The hydrolyzate was passed through a sieve of 160 mesh, and the filtrate was concentrated under vacuum at a concentration of 20 ° C and a vacuum of 80 kPa to obtain a concentrate.

Example 19: A method for rapidly preparing an Antarctic krill concentrate and powder on board, the steps are as follows: The fresh Antarctic krill is drained and crushed, and the crushed material is passed through 20 watts, a wavelength of 270 nm, and a distance of 1 m of ultraviolet irradiation. The crushed material was autolyzed for 5 hours at ambient temperature in the fishing area after 30 minutes of ultraviolet irradiation. After 2 times the volume of water in the low temperature autolyzed slurry and treated with 800 watts of ultrasonic wave (365 nm) for 10 minutes, the pH of the crushed suspension was adjusted to 7.5 with 1 M of HCl or NaOH. The low temperature protease to which 10 ⁶ U/kg of substrate was added to the homogenate was digested at 10 ° C for 4 hours. The hydrolyzate was passed through a sieve of 200 mesh, and the filtrate was concentrated under vacuum at a concentration of 25 ° C and a vacuum of 85 kPa to obtain a concentrate.

Example 20: Rapid preparation method of Antarctic krill concentrate and powder on board, the steps are as follows: The fresh Antarctic krill is drained and crushed, and the broken material is passed through 20 watts, the wavelength is 278 nm, and the ultraviolet light is 1 meter away. The crushed material was irradiated for 30 minutes and irradiated for 5 hours at 4 ° C (ambient temperature in the fishing area) after ultraviolet irradiation. After 3 times the volume of water in the low-temperature autolyzed slurry and treated with 800 watts of ultrasonic wave (378 nm) for 15 minutes, the p!Hi~8.5 of the suspension suspension was adjusted with 1 M of HCl or NaOH. The low temperature protease containing ⁹ x 105 U/kg substrate in the homogenate was digested at 20 °C for 4 hours. The hydrolyzate was passed through a sieve of 200 mesh, and the filtrate was concentrated under vacuum at a concentration of 25 ° C and a vacuum of 90 kPa to obtain a concentrate.

Example 21: On-board rapid preparation method of Antarctic krill concentrate and powder, the steps are as follows: The fresh Antarctic krill is drained and crushed, and the crushed material is crushed by 3 watts, a wavelength of 290 nm, and a distance of 0.5 m of ultraviolet light. After 10 minutes of UV irradiation, it was autolyzed for 1 hour at the ambient temperature of the fishing area. After 1 volume of water in the low temperature autolyzed slurry and treated with 300 watts of ultrasonic wave (389 nm) for 5 minutes, the pH of the crushed suspension was adjusted to 7.5 with 1 M of HCl or NaOH. A low temperature protease containing ⁹ x 105 U/kg of substrate was added to the homogenate for 1 hour at 10 °C. The hydrolyzate was passed through a sieve of 120 mesh, and the filtrate was concentrated under vacuum at a concentration of 20 ° C and a vacuum of 75 kPa to obtain a concentrate.

Example 22: On-board rapid preparation method of Antarctic krill concentrate and powder, the steps are as follows: The fresh Antarctic krill is drained and crushed, and the crushed material is crushed by 40 watts, a wavelength of 295 nm, and a distance of 1 m of ultraviolet light. After 30 minutes of UV irradiation, it was autolyzed for 5 hours at the ambient temperature of the fishing area. After 2 times the volume of water in the autolyzed slurry at low temperature and treated with 800 watts of ultrasonic wave (498 nm) for 10 minutes, the pH of the suspension suspension was adjusted to 7.5 with 1 M HCl or NaOH. The low temperature protease to which 10 ⁶ U/kg of substrate was added to the homogenate was digested at 10 ° C for 4 hours. The hydrolyzate was passed through a 200-mesh sieve, and the filtrate was spray-dried at a drying tower temperature of 150 to 180 ° C and an exhaust gas temperature of 75 to 95 ° C to obtain a dry powder.

Example 23: On-board rapid preparation method of Antarctic krill concentrate and powder, the steps are as follows: The fresh Antarctic krill is drained and crushed, and the crushed material is crushed by 40 watts, a wavelength of 300 nm, and irradiated by ultraviolet light at a distance of 1 m. After 30 minutes of UV irradiation, it was autolyzed for 5 hours at the ambient temperature of the fishing area. After 3 times the volume of water in the autolyzed slurry at low temperature and treated with 800 watts of ultrasonic wave (400 nm) for 15 minutes, the pH of the suspension suspension was adjusted to 8.5 with 1 M HCl or NaOH. A low temperature protease containing 9 X 10 ⁵ U/kg of substrate was added to the homogenate for 4 hours at 20 °C. The hydrolyzate was passed through a 200-mesh sieve, and the filtrate was spray-dried at a drying tower temperature of 180 ° C and an exhaust gas temperature of 95 ° C to obtain a dry powder.

The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto, and any technical person skilled in the art within the technical scope disclosed by the present invention, the technical solution according to the present invention Equivalent substitutions or modifications of the inventive concept are intended to be included within the scope of the invention.

Claims

A method for preparing shrimp oil based on Antarctic krill, characterized in that it comprises the following steps:

51. Raw material processing: The harvested Antarctic krill is drained, homogenized or crushed to obtain krill raw materials;

52. Autolysis: After irradiating the krill raw material with ultraviolet rays, it is autolyzed at 0 to 4 ° C to obtain a self-solution;

53. Ultrasonic treatment: treating the self-solution after adding water with ultrasonic waves;

54. Exogenous enzyme enzymatic hydrolysis: adjusting the pH value of the self-solution to 7.5-8.0, adding alkaline protease to the self-solution, and enzymatically decomposing at 55-65 ° C to obtain an enzymatic hydrolysate;

55. Extracting shrimp oil and fat based on the enzymatic hydrolysate using ethanol or n-ethane.

The method for preparing shrimp oil based on Antarctic krill according to claim 1, wherein the alkaline protease is a Bacillus subtilis alkaline protease.

The method for preparing shrimp oil and fat based on Antarctic krill according to claim 2, wherein the ultrasonic wave in step S3 is selected from a power of 300 to 800 watts and a wavelength of 200 to 400 nanometers, and the self-solution is treated for 10-30 minutes. .

The method for preparing shrimp oil and fat based on Antarctic krill according to claim 3, wherein the ultraviolet light in step S2 is selected from the group consisting of 20 to 40 watts, a wavelength of 200 to 300 nanometers, a distance of 0.5 to 1 meter, and irradiation of 10~. 35 minutes.

The method for preparing shrimp oil and fat based on Antarctic krill according to claim 4, wherein the step S2 is autolyzed for 2 to 10 hours after the ultraviolet treatment.

6. The method for preparing shrimp oil based on Antarctic krill according to claim 5, wherein the pH value is adjusted, and HC1 or NaOH having a concentration of 1 to 6 M is selected.

7. The method for preparing shrimp fat based on Antarctic krill according to claim 6, comprising the steps of:

Step Sl, Antarctic krill pulping, obtaining a slurry;

Step S2: irradiating the slurry with ultraviolet rays for 25 to 35 minutes and then autolyzing to obtain the self-solution; and then performing high temperature autolysis, specifically comprising the following four steps:

S2-l, adding 0.5 to 1 volume of water to the self-solution, respectively, and the final concentration is

2.0~2.5% sodium chloride solution and a final concentration of 0.05-0.1% ethanol; S2-2, again using the power of 20~40 watts, wavelength 200~300 nm, distance 0.5~1 m ultraviolet radiation to the self-solution 10~40 minutes;

S2-3, adjusting the pH of the self-solution to 7.5~8.0;

S2-4, heating the solution from a temperature of 3 to 8 ° C every 10 to 30 minutes to a temperature of

30-65 °C, autolysis 2~8 hours;

Step S3, adding 0.5-1.5 volumes of water to the solution, and treating with ultrasonic waves; Step S4, adjusting the pH value, adding alkalinity of 9~10x10 ⁵ U/kg substrate to the self-solution Protease, enzymatically obtained for 1 to 4 hours to obtain the enzymatic hydrolysate; Step S5, adding 0.5 to 1.5 volumes of n-hexane to the enzymatic hydrolysate, allowing to stand for 30 to 90 minutes, and centrifuging to obtain a first upper liquid phase and a first precipitate; after removing the n-hexane from the first upper liquid phase Get shrimp oil.

The method for preparing shrimp oil and fat based on Antarctic krill according to claim 7, wherein in step S5, after obtaining the first precipitate, 0.4 to 0.6 times of water is added to the first precipitate. In the drying tower, the inlet air temperature is 140-170 ° C, and the exhaust temperature is 60-90 ° C for spray drying to obtain a dry powder.

The method for preparing shrimp oil and fat based on Antarctic krill according to claim 7, wherein in step S5, 0.5 to 1.5 volumes of n-hexane is added to the first precipitate, and the mixture is allowed to stand for 20 to 60 minutes. And centrifuging to obtain a second upper liquid phase and a second precipitate; after mixing the first upper liquid phase with the second upper liquid phase, removing n-hexane to obtain shrimp oil.

10. The method for preparing shrimp oil and fat based on Antarctic krill according to claim 9, wherein in step S5, after obtaining the second precipitate, 0.4 to 0.6 times of water is added to the second precipitate, The drying tower has an inlet air temperature of 140 to 170 ° C and a discharge temperature of 60 to 90 ° C for spray drying to obtain a dry powder.

The method for preparing shrimp oil and fat based on Antarctic krill according to any one of claims 7 to 10, wherein in the step S5, the method for removing the n-hexane is: removing the positive one at 30 to 45 ° C under reduced pressure. alkyl.

12. The method for preparing shrimp fat based on Antarctic krill according to claim 6, comprising the steps of:

Step Sl, Antarctic krill pulping, obtaining a slurry;

Step S2, irradiating the homogenate with ultraviolet rays for 10 to 30 minutes and then autolyzing;

Step S3, adding 1-3 times the volume of water to the solution, and treating with ultrasonic waves;

Step S4, after adjusting the pH, adding 9-10×10 ⁵ U/kg of the substrate to the self-solution, and enzymatically hydrolyzing for 1 to 4 hours to obtain the hydrolysate;

Step S5, the enzymatic hydrolysate is spray-dried to obtain a dry powder; adding 5 to 15 volumes of 90% ethanol to the dry powder, mixing, standing for 30 to 90 minutes, and centrifuging to obtain a third upper liquid phase And a third precipitate; the third liquid phase removes ethanol to obtain an Antarctic krill phospholipid.

13. The method for preparing shrimp oil and fat based on Antarctic krill according to claim 12, wherein in step S5, after obtaining the third precipitate, first adding 2.5 to 7.5 times the volume to the third precipitate Ethanol, mixing, standing for 20 to 60 minutes, centrifuging to obtain a fourth upper liquid phase and a fourth precipitate; mixing the third upper liquid phase and the fourth upper liquid phase, removing ethanol to obtain an antarctic phosphorus worm Phospholipids

The method for preparing shrimp oil and fat based on Antarctic krill according to claim 12 or 13, wherein the method for removing ethanol in step S5 is: removing ethanol at a concentration of 40 to 60 ° C under reduced pressure, and recovering ethanol.

15. A method of preparing shrimp oil based on Antarctic krill according to claim 14, wherein steps S1 and S2 are operated on a fishing vessel;

And between the steps S2 and S3, further comprising the following operations on the fishing vessel: directly freezing the homogenate; correspondingly the step S3 first melting the frozen colloidal slurry to prepare the hook Slurry Status.

16. A method for preparing shrimp oil based on Antarctic krill according to claim 14, wherein steps S1 and S2 are operated on a fishing vessel;

And between the steps S2 and S3, further comprising the following operations on the fishing vessel: the homogenate is spray-dried to obtain a dry powder; the corresponding step S3 first dissolves the dry powder in a volume of 7 to 12 times. The slurry is returned to the state of the slurry.

17. A method for preparing shrimp concentrate and shrimp powder based on Antarctic krill, characterized in that it comprises the following steps:

54, exogenous enzymatic hydrolysis: adjusting the pH of the solution to from 7.5 to 8.5, was added from a low temperature protease 9 ~ 10x l0 ⁵ U / kg of substrate to the enzyme at 10 ~ 20 ° C Obtaining the enzymatic hydrolysate after 1-4 hours;

55, the enzymatic hydrolysate is filtered through 120~200 mesh; the filtrate or concentrated in low temperature vacuum to obtain 40~70% of the Antarctic krill concentrate, or after spray drying Krill dry powder.

18. A method of preparing shrimp concentrate, shrimp powder based on Antarctic krill according to claim 17, wherein said low temperature protease is a wave low temperature protease.

The method for preparing shrimp concentrate and shrimp powder based on Antarctic krill according to claim 18, wherein the ultrasonic wave in step S3 is a power of 300 to 800 watts and a wavelength of 200 to 400 nanometers, and the self-solution is processed. 5~15 minutes.

The method for preparing shrimp concentrate and shrimp powder based on Antarctic krill according to claim 19, wherein the ultraviolet light in step S2 is 20 to 40 watts in power, 200 to 300 nm in wavelength, and 0.5 to 1 meter in distance. , Irradiation for 10 to 30 minutes.

A method for preparing shrimp concentrate and shrimp powder based on Antarctic krill according to claim 20, wherein the step S2 is autolyzed for 1 to 5 hours after the ultraviolet treatment.

22. The method for preparing shrimp concentrate and shrimp powder based on Antarctic krill according to claim 21, wherein the pH value is adjusted, and HC1 or NaOH having a concentration of 1 to 6 M is selected.

The method for preparing shrimp concentrate and shrimp powder based on Antarctic krill according to any one of claims 17 to 22, wherein in step S5, the low temperature vacuum is performed at a concentration temperature of 20 to 25 ° C and a vacuum degree of 75 to 90 kPa. Concentrate to obtain a concentrate.

The method for preparing shrimp concentrate and shrimp powder based on Antarctic krill according to any one of claims 17 to 22, wherein in step S5, the inlet air temperature of the drying tower is 150 to 180 ° C, and the exhaust gas temperature is Spray drying at 75~95 °C to produce a dry powder.