JP5357362B2 - Method for producing krill oil or krill concentrate, krill meal from nankoku krill - Google Patents

Abstract

Methods for preparing the shrimp grease and preparing the shrimp concentrate or the shrimp med from the Euphausia superba include the following steps: homogenizing or crushing the Euphausia superba to obtain the krill raw material; autolyzing the krill raw material at 0-4? after ultraviolet irradiation to get the solution; treating the autolyzed solution by ultrasonic; adjusting the PH value of the solution and realizing the enzymolysis by adding exogenous enzymes; and extracting the krill grease, or obtaining the shrimp concentrate after condensing the filtered solution of the enzymolysis solution or the shrimp med after spray drying the filtered solution. The methods have the following advantages: simple operation, high efficiency, and the trophicity and functionality of the product by maximally saving some original biologically active substances.

Description

  The present invention relates to a method for extracting a product from Antarctic krill, and specifically to a method for extracting krill oil or krill concentrate and krill meal.

  The Antarctic krill (Euphausia superba) is one of the largest single species resources on earth, with an estimated surviving amount of about 400-1.5 billion tons, and the annual production of mature krill is 300-500 million The amount that can be captured per year can reach about 100 million tons, forming a huge potential fishery resource. In recent years, as the traditional fishery resources are gradually depleted in the world, and as the 200 nautical miles exclusive economic zone is submitted, the huge Antarctic waters resources in Antarctic waters are attracting attention in advanced ocean fishing countries. In Japan, Antarctic krill resources are listed as one of the main development varieties for the future development of pelagic fisheries.

  At present, the development of Antarctic krill resources mainly uses the protein, but the development and utilization of Antarctic krill oil is still insufficient. Antarctic krill lives in boreal waters, and krill oil is rich in phospholipids and can account for over 40% of the total oil. Antarctic krill phospholipids are rich in omega-3 polyunsaturated fatty acids such as DHA and EPA. Studies show that omega-3 polyunsaturated fatty acids have improved prognosis in patients with heart disease, increased fetal growth rate, inhibition of tumor growth and metastasis, anti-inflammation, platelet aggregation inhibition, anti-hypertension and anti-high fat It has beneficial effects on a wide range of health, such as regulation of blood glucose and the sensitivity of the body to insulin. Japanese scholars point out that omega-3 polyunsaturated fatty acids present in the form of phospholipids are more prevalent in cardiovascular activity. This proves that the nutritional and medicinal value of Antarctic krill phospholipids is very high and has good development and future prospects.

  Since Antarctica is far away, it must be transported and stored for a long time after capturing Antarctic krill. Antarctic krill is rich in polyunsaturated fatty acids and is therefore subject to spoilage and alteration. Therefore, after capturing the Antarctic krill, it can only be frozen and cryopreserved quickly to guarantee the original quality of the phospholipid, greatly increasing the transportation and storage costs of the Antarctic krill, and the Antarctic krill phospholipid. It becomes a bottleneck that restricts the development and use of resources. In order to solve the problem that transport and storage of Antarctic krill is difficult, the optimal method is to rapidly process Antarctic krill after production to produce phospholipids. There is no way to process and manufacture krill products on board.

  The present invention has been made for the above-mentioned problems, and is suitable for the extraction of beneficial substances of Antarctic krill after capturing Antarctic krill to produce a product, particularly for direct operation on a fishing boat. The purpose is to provide a simple method.

  In order to achieve the above object, the present invention first provides a basic method for producing krill oil from Antarctic krill comprising the following steps.

  Step 1. A raw material treatment step of draining the captured Antarctic krill and then homogenate or press crushing to obtain a krill raw material.

  Step 2. A self-digestion step of obtaining a self-digestion solution by self-digestion at 0 to 4 ° C. after irradiating the krill raw material with ultraviolet rays.

  Step 3. An ultrasonic treatment step for treating the autolyzed liquid after being hydrated with ultrasonic waves.

  Step 4. An enzyme by an exogenous enzyme that adjusts the pH value of the autolysate to 7.5 to 8.0, adds alkaline protease to the autolysate, and enzymatically decomposes at 55 to 65 ° C. to obtain an enzyme digestion solution Disassembly step.

  Step 5. Extracting krill oil from the enzymatic decomposition solution using ethyl alcohol or n-hexane;

  In a preferred form, the alkaline protease is a Bacillus subtilis alkaline protease. In step 3, the ultrasonic wave having a power of 300 to 800 watts and a wavelength of 200 to 400 nm is used, and the time for treating the autolysate is 10 to 30 minutes. In Step 2, the ultraviolet rays having a power of 20 to 40 watts, a wavelength of 200 to 300 nm and an irradiation distance of 0.5 to 1 m are used, the treatment time is 10 to 35 minutes, and after the treatment with ultraviolet rays, The time for autolysis is 2 to 10 hours. In the above step, the pH value is adjusted by selecting HCl or NaOH having a concentration of 1 to 6M.

  An embodiment for producing a preferred krill oil according to the present invention based on the above basic method includes the following steps.

  Step 1. A step of homogenizing the Antarctic krill to obtain a homogenate solution.

  Step 2. After the ultraviolet light is irradiated to the homogenate liquid for 25 to 35 minutes and then self-digested to obtain the self-digested liquid, high-temperature self-digestion is performed (for the purpose of improving product yield). Includes four steps.

  Step 2-1. Adding 0.5 to 1 volume of water, sodium chloride solution having a final concentration of 2.0 to 2.5%, and ethyl alcohol having a final concentration of 0.05 to 0.1% to the autolysate, respectively.

  Step 2-2. The step of irradiating the self-digesting liquid again for 10 to 40 minutes with ultraviolet rays having a power of 20 to 40 watts, a wavelength of 200 to 300 nm and a distance of 0.5 to 1 m.

  Step 2-3. Adjusting the pH value of the autolysate to 7.5-8.0.

  Step 2-4. The step of heating the self-digestion liquid to 30-65 ° C at a rate of 3-8 ° C improvement every 10-30 minutes and self-digesting for 2-8 hours.

  Step 3. Adding 0.5 to 1.5 times volume of water to the autolysate and treating with ultrasound.

Step 4. After adjusting the pH value, adding 9-10 × 10 ⁵ U / kg substrate alkaline protease to the autolysate and enzymatically decomposing for 1-4 hours to obtain the enzyme digestion solution.

  Step 5. 0.5-1.5 times volume of n-hexane is added to the enzymatic decomposition solution, left to stand for 30-90 minutes, and centrifuged to obtain a first upper liquid phase and a first precipitate. A step of obtaining krill oil after removing n-hexane from the upper liquid phase.

  In a preferred embodiment, as an improvement of the aspect of producing the krill oil, in Step 5, after obtaining the first precipitate, 0.4 to 0.6 times volume of water is added to the first precipitate. Then, spray drying is performed at a drying tower intake temperature of 140 to 170 ° C. and an exhaust temperature of 60 to 90 ° C. to produce a dry meal, which is transported back to the land and used as a raw material for extracting nutrient components. In Step 5, as a method for removing n-hexane, the solution is concentrated under reduced pressure at 30 to 45 ° C. to remove n-hexane, and at the same time, n-hexane is recovered to achieve the purpose of circulation use.

  In order to further improve the production yield, as an improvement of the aspect of producing the krill oil, in step 5, 0.5 to 1.5 times volume of n-hexane is added to the first precipitate, Allow to stand for 20-60 minutes, centrifuge to obtain a second upper liquid phase and a second precipitate, and after mixing the first upper liquid phase and the second upper liquid phase, n-hexane To obtain krill oil. After performing high temperature autolysis and secondary precipitation, the extraction rate for extracting krill oil can reach 88.79-94.56%. Therefore, the present invention reduces the operating cost, greatly improves the extraction efficiency of krill oil, saves the transportation cost of Antarctic krill, guarantees the quality of krill products, and is used for on-site operation on a fishing boat. It is very suitable and has high economic effects and benefits.

  Further, 0.4 to 0.6 times the volume of water is added to the second precipitate, and spray drying is performed at a drying tower intake temperature of 140 to 170 ° C. and an exhaust temperature of 60 to 90 ° C. to produce a dry meal. Still, it can be transported back to the land and used as a raw material for extracting nutrients.

  Based on the above basic method, the present invention further provides an embodiment for producing a preferred krill phospholipid, comprising the following steps.

  Step 1. A step of homogenizing the Antarctic krill to obtain a homogenate solution.

  Step 2. Self-digesting after irradiating the homogenate solution with ultraviolet rays for 10 to 30 minutes.

  Step 3. Adding 1 to 3 volumes of water to the autolysate and treating with ultrasound.

Step 4. After adjusting pH, adding the alkaline protease of 9-10 * 10 < ⁵ > U / kg substrate to the said autolysis liquid, and carrying out the enzymatic decomposition at 55-65 degreeC for 1-4 hours, and obtaining the said enzymatic decomposition liquid.

  Step 5. After spray-drying the enzyme decomposition solution, a dry meal is obtained, and 5-15 times volume of 90% ethyl alcohol is added to the dry meal, mixed uniformly, left to stand for 30-90 minutes, and centrifuged. A third upper liquid phase and a third precipitate are obtained, and ethyl alcohol in the third liquid phase is removed to obtain Antarctic krill phospholipids.

  In a preferred embodiment, as an improvement of the aspect of producing the above krill phospholipid, in order to further improve the production yield, in Step 5, after obtaining the third precipitate, first, the third precipitate is added to 2.5. -7.5 times volume of ethyl alcohol was added, mixed uniformly, allowed to stand for 20-60 minutes, centrifuged to obtain a fourth upper liquid phase and a fourth precipitate, The upper liquid phase and the fourth upper liquid phase are mixed, and ethyl alcohol is removed to obtain Antarctic krill phospholipids. In step 5, as a method for removing ethyl alcohol, the alcohol is concentrated under reduced pressure at 40 to 60 ° C. to remove ethyl alcohol and to recover ethyl alcohol.

Moreover, as an aspect for producing the above krill phospholipid, steps 1 and 2 are operated on a fishing boat, and between steps 2 and 3, the autolysate is directly frozen and stored on the fishing boat. . Correspondingly, in step 3, the frozen and stored self-digestion solution can be first thawed and returned to the self-digestion state, and can be operated on land or on a fishing boat.

Moreover, as an aspect which manufactures the said krill phospholipid, step 1 and step 2 operate on a fishing boat, and between step 2 and step 3, after spray-drying the said autolysis liquid on a fishing boat, dry meal And save it. Correspondingly, in step 3, the operation is performed on land or on a ship, and the dried meal is first dissolved in 7 to 12 times the volume of water and returned to the state of autolysate .

  The present invention further provides a method for producing krill concentrate, krill meal from Antarctic krill, comprising the following steps.

  Step 1. A raw material treatment step of draining the captured Antarctic krill and then homogenating or press crushing to obtain a krill raw material.

  Step 2. A self-digestion step of obtaining a self-digestion solution by self-digestion at 0 to 4 ° C. after irradiating the krill raw material with ultraviolet rays.

  Step 3. An ultrasonic treatment step for treating the autolyzed liquid after being hydrated with ultrasonic waves.

Step 4. The pH value of the autolysate is adjusted to 7.5 to 8.5, 9 to 10 × 10 ⁵ U / kg substrate of low temperature protease is added to the autolysate, and the mixture is heated at 10 to 20 ° C. for 1-4 hours. Enzymatic degradation step using an exogenous enzyme to obtain an enzymatic degradation solution by enzymatic degradation.

  Step 5. The enzyme decomposition solution is passed through a 120-200 mesh sieve to obtain a filtrate, and the filtrate is subjected to low-temperature vacuum concentration to obtain a Antarctic krill concentrate occupying 40% to 70% of a solid, Or the step of obtaining a krill dry meal after spray drying.

In a preferred form, said cold protease in step 4 selects Novozymes (China) Ltd. made Novozymes Polarzyme (TM). In step 3, the ultrasonic wave has a power of 300 to 800 watts and a wavelength of 200 to 400 nm, and the autolyzed liquid is treated for 5 to 15 minutes. In step 2, the ultraviolet light is 20 to 40 watts and has a wavelength of 200 to 300 nm, irradiated at a distance of 0.5 to 1 m, treated for 10 to 30 minutes, and treated with ultraviolet light for 1 to 5 hours. Digest. Select a concentration of 1-6 M HCl or NaOH to adjust the pH value.

  In a preferred form, in Step 5, a concentrate is produced by performing low-temperature vacuum concentration at a concentration temperature of 20 to 25 ° C. and a degree of vacuum of 75 kPa to 90 kPa, or a drying tower intake temperature of 150 to 180 ° C., and an exhaust temperature of 75 to 95. Spray drying is performed at 0 ° C. to produce a dry meal.

  In the present invention, “draining” is intended to remove water adhering to fresh Antarctic krill, and after draining, krill itself is still kept in the state of fresh krill. Moreover, ultraviolet irradiation aims at increasing the self-digestion ability of Antarctic krill. Sonication aims to increase the enzymatic degradation capacity.

  The present invention is the first time to apply self-digestion technology and ultrasonic technology simultaneously to the extraction method of Antarctic krill oil, the operation of the extraction process is simple, the efficiency is high, the product purity and quality are all good, and suitable for popularization Have high economic effect and profit. Because it uses the strong self-digestion ability of fresh Antarctic krill itself, it reduces the amount of exogenous enzyme used and the production cost. In addition, since ultrasonic treatment is adopted, the extraction rate of Antarctic krill oil is improved, and the effect and profit are improved. The method according to the present invention is mild in conditions, and retains some of the original physiologically active materials in Antarctic krill oil to make the product both nutritious and functional. The present invention has the following advantages.

  1. The operation process according to the present invention is simple, does not require complicated equipment, and is suitable for performing on a fishing boat.

  2. The present invention makes use of the strong self-digestion ability of the Antarctic krill itself, thereby reducing the amount of exogenous enzyme used and reducing production costs.

  3. The present invention is the first to apply ultrasonic technology to extraction of Antarctic krill oil, greatly improving the yield of Antarctic krill oil, and improving the economic effect and profit.

  4). The overall temperature of the operation process of the present invention is relatively low, reducing energy consumption and maximally retaining some of the original physiologically active material in Antarctic krill oil.

  5. The present invention utilizes cold protease for the first time in the production of Antarctic krill concentrates and meals, and the overall temperature of the operation process is relatively low, reducing energy consumption and maximally reducing some of the original in Antarctic krill oil. A physiologically active material can be retained.

  6). The present invention uses a press crushing technique for the production of Antarctic krill concentrate and meal for the first time, and the degree of destruction of the technique to Antarctic krill shells is light and reduces the release of fluorine in krill shells to the maximum.

  Example 1: An onboard processing method for producing krill oil from Antarctic krill, comprising the following steps.

  1. Ingredients and processing: Freshly captured Antarctic krill is drained and then homogenized directly.

  2. Low-temperature self-digestion: Treated Antarctic krill homogenate with UV power of 20 to 40 Watts and wavelength of 200 to 300 nm and irradiation for 25 to 35 minutes at a distance of 0.5 to 1 m, and processed homogenate to 0 Place at -4 ° C (average environmental temperature in the Antarctic area during the capture season) and self-digest for 2-10 hours.

  3. High-temperature self-digestion: 0.5 to 1 volume of water, sodium chloride (final concentration 2.0% to 2.5%) and ethyl alcohol (0.05% each) were added to the homogenate solution after the treatment of 2 above. ~ 0.1% homogenate liquid volume) is added, treated with ultraviolet light with a power of 20-40 watts and a wavelength of 200-300 nm and irradiated for 10-40 minutes at a distance of 0.5-1 m, 1-6 M The pH value of the homogenate solution is adjusted to 7.5 to 8.0 with HCl or NaOH, and the homogenate solution is heated to 30 to 65 ° C. at a rate of 3 to 8 ° C. every 10 to 30 minutes. Self-digest time.

4). Enzymatic degradation with an exogenous enzyme assisted by ultrasound: 0.5 to 1.5 times volume of water is added to the homogenate solution after the treatment of 3 above, mixed uniformly, and ultra-300-800 Watts Treated with sonic waves (wavelength 200 to 400 nm) for 10 to 30 minutes, adjusted pH to 7.5 to 8.0 with 1 to 6 M HCl or NaOH, and added alkaline protease (9 to 10 × 10 ⁵ U / h) to the homogenate solution. kg substrate), uniformly mixed, and enzymatically degraded at 55-65 ° C. for 1-4 hours.

  5. Extraction of krill oil: Add 0.5-1.5 times volume of n-hexane to the enzyme digestion solution, mix uniformly, let stand for 30-90 minutes, centrifuge in sedimentation centrifuge, upper layer Obtain liquid phase A and precipitate A; add 0.5-1.5 times volume of n-hexane to precipitate A, mix uniformly, let stand for 20-60 minutes, and centrifuge in precipitation centrifuge. Thus, upper liquid phase B and precipitate B are obtained; liquid phase A and liquid phase B are mixed and concentrated under reduced pressure at 30 to 45 ° C. to remove n-hexane to obtain krill oil. Hexane can be used in duplicate. 0.4 to 0.6 times the volume of water is added to the precipitate B, spray drying is performed at a drying tower intake temperature of 140 to 170 ° C., and an exhaust temperature of 60 to 90 ° C. to produce a dry meal, which is then transported to land. Return to the raw material to extract protein and other nutrients.

  Example 2: An onboard processing method for producing krill oil from Antarctic krill, comprising the following steps.

  The freshly caught Antarctic krill is drained and then homogenized directly. The homogenate is treated by irradiating UV light with a power of 40 watts, a wavelength of 245 nm, and a distance of 0.5 m for 25 minutes. Cryogenic self digestion at temperature for 2 hours. Each of the homogenates after low-temperature self-digestion treatment is 0.5 times the volume of water, NaCl (final concentration 2.0-2.5%) and ethyl alcohol (final concentration 0.05-0.1%). The mixture is uniformly mixed, and the homogenate solution is irradiated with ultraviolet rays having a power of 20 watts, a wavelength of 299 nm and a distance of 0.5 m for 10 minutes, and the pH value of the homogenate solution is 7.5 to 8 with 1 M HCl or NaOH. The temperature is raised to 30 ° C. at a rate of 8 ° C. every 30 minutes, and high temperature self-digestion is performed for 8 hours under these conditions.

Add 0.5 volumes of water to the homogenate after high-temperature self-digestion, mix evenly, treat with 300 watts of ultrasonic power (295 nm) for 10 minutes, homogenate with 1M HCl or NaOH The pH value of the solution is adjusted to 7.5 to 8.0, and alkaline protease (9 × 10 ⁵ U / kg substrate) is added to the homogenate solution, mixed uniformly, and enzymatically degraded at 55 ° C. for 1 hour.

  Add 0.5-fold volume of n-hexane to the enzyme decomposition solution, mix uniformly, let stand for 30 minutes, and centrifuge in a precipitation centrifuge to obtain upper liquid phase A and precipitate A. 0.5-fold volume of n-hexane is added to the precipitate A, mixed uniformly, allowed to stand for 20 minutes, and centrifuged with a precipitation centrifuge to obtain an upper liquid phase B and a precipitate B. Liquid phase A and liquid phase B are mixed and concentrated under reduced pressure at 35 ° C. to remove n-hexane, and then krill oil is obtained. The recovered n-hexane can be used in duplicate. The precipitate A and the precipitate B are mixed, 0.6 volume of water is added, and then it is spray-dried under conditions of a drying tower intake temperature of 140 to 170 ° C. and an exhaust temperature of 60 to 90 ° C., Dry meal is produced and transported back to land for use as a raw material for extracting protein and other nutrients.

  Example 3 An onboard processing method for producing krill oil from Antarctic krill comprising the following steps.

  The freshly caught Antarctic krill is drained and then homogenized directly. The homogenate solution is irradiated with UV light with a power of 40 watts, a wavelength of 230 nm and a distance of 1 m for 25 minutes, and is subjected to low temperature self-digestion at 0 to 4 ° C. for 10 hours. To do. Add 1 volume of water, NaCl (final concentration 2.0%) and ethyl alcohol (final concentration 0.05%) to the homogenate solution after low-temperature self-digestion, respectively, and mix uniformly. Is irradiated with UV light of power 40 watts, wavelength 295 nm, distance 0.5 m for 30 minutes, the pH value of the homogenate solution is adjusted to 7.5-8.0 with 4M HCl or NaOH, and further 10 minutes Every time, the temperature is raised to 30 ° C. at a rate of 5 ° C., and high temperature self-digestion is performed for 2 hours under these conditions.

Add 1.5 times the volume of water to the homogenate solution, mix uniformly, treat with ultrasonic power (390 nm) at 500 watts for 13 minutes, and adjust the pH value of the homogenate solution to 7.5 to 4M with HCl or NaOH. Adjust to 8.0, add alkaline protease (9 × 10 ⁵ U / kg substrate) to the homogenate, mix uniformly, and enzymatically digest at 55 ° C. for 2 hours.

  1-fold volume of n-hexane is added to the enzyme decomposition solution, mixed uniformly, left to stand for 60 minutes, and centrifuged with a precipitation centrifuge to obtain upper liquid phase A and precipitation A. 1-fold volume of n-hexane is added to precipitate A, mixed uniformly, allowed to stand for 20 minutes, and centrifuged with a precipitation centrifuge to obtain upper liquid phase B and precipitate B. Liquid phase A and liquid phase B are mixed and concentrated under reduced pressure at 38 ° C. to remove n-hexane, and then krill oil is obtained. The recovered n-hexane can be used in duplicate. Precipitation A and Precipitation B are mixed and 0.6 times the volume of water is added. Then, it is spray-dried under conditions of a drying tower intake temperature of 145 ° C and an exhaust temperature of 65 ° C to produce a dry meal. , Transported back to the land and used as a raw material to extract proteins and other nutrients.

  Example 4: An onboard processing method for producing krill oil from Antarctic krill, comprising the following steps.

  The freshly caught Antarctic krill was drained and then homogenized directly. The homogenate solution was irradiated with ultraviolet rays with a power of 27 watts, a wavelength of 245 nm, and a distance of 0.5 m for 30 minutes, and cooled at 0 to 4 ° C. for 6 hours. Self-digest. Add 1 volume each of water, NaCl (final concentration 2.0-2.5%) and ethyl alcohol (final concentration 0.05-0.1%) to the homogenate after the low temperature self-digestion treatment. The homogenate liquid is uniformly mixed, and the homogenate liquid is treated by irradiating ultraviolet rays with a power of 40 watts, a wavelength of 200 nm and a distance of 0.5 m for 10 minutes, and the pH value of the homogenate liquid is 7.5 to 8.0 with 1 M HCl or NaOH. In addition, the temperature is increased to 30 ° C. at a rate of 3 ° C. every 10 minutes, and high temperature self-digestion is performed for 2 hours under these conditions.

Add 1 volume of water to the homogenate after high-temperature self-digestion, mix uniformly, treat with ultrasonic power (wavelength 389 nm) for 21 minutes, homogenate with 1M HCl or NaOH. The pH value is adjusted to 7.5 to 8.0, alkaline protease (9 × 10 ⁵ U / kg substrate) is added to the homogenate solution, mixed uniformly, and enzymatically degraded at 65 ° C. for 4 hours.

  Add 0.5-fold volume of n-hexane to the enzyme decomposition solution, mix uniformly, let stand for 30 minutes, and centrifuge in a precipitation centrifuge to obtain upper liquid phase A and precipitate A. 0.5-fold volume of n-hexane is added to the precipitate A, mixed uniformly, allowed to stand for 20 minutes, and centrifuged with a precipitation centrifuge to obtain an upper liquid phase B and a precipitate B. Liquid phase A and liquid phase B are mixed, concentrated under reduced pressure at 40 ° C. to remove n-hexane, and then krill oil is obtained. The recovered n-hexane can be used in duplicate. Precipitation A and Precipitation B are mixed and 0.6 times the volume of water is added. Then, it is spray-dried under conditions of a drying tower intake temperature of 160 ° C. and an exhaust temperature of 85 ° C. to produce a dry meal. , Transported back to the land and used as a raw material to extract proteins and other nutrients.

  Example 5: An onboard processing method for producing krill oil from Antarctic krill, comprising the following steps.

  The freshly caught Antarctic krill was drained and then homogenized directly. The homogenate solution was irradiated with UV light at a power of 33 watts, a wavelength of 210 nm, and a distance of 0.5 m for 30 minutes. Self-digest at low temperature for 2 hours at the average environmental temperature in the Antarctic region Add 1 volume each of water, NaCl (final concentration 2.0-2.5%) and ethyl alcohol (final concentration 0.05-0.1%) to the homogenate after the low temperature self-digestion treatment. The homogenate solution was uniformly mixed and treated by irradiating ultraviolet rays with a power of 40 watts, a wavelength of 267 nm, and a distance of 0.5 m for 40 minutes, and the pH value of the homogenate solution was 7.5 to 8.0 with 1 M HCl or NaOH. The temperature is further increased to 50 ° C. at a rate of 3 ° C. every 10 minutes, and high temperature self-digestion is performed for 8 hours under these conditions.

Add 0.5 volumes of water to the homogenate after autolysis, mix uniformly, treat with 500 watts of ultrasonic power (380 nm) for 27 minutes, and homogenate with 1M HCl or NaOH. Is adjusted to 7.5-8.0, alkaline protease (9 × 10 ⁵ U / kg substrate) is added to the homogenate, mixed uniformly, and enzymatically degraded at 65 ° C. for 3 hours.

  1.5-fold volume of n-hexane is added to the enzymatic decomposition solution, mixed uniformly, allowed to stand for 60 minutes, and centrifuged with a precipitation centrifuge to obtain upper liquid phase A and precipitate A. 1.5-fold volume of n-hexane is added to precipitate A, mixed uniformly, allowed to stand for 60 minutes, and centrifuged with a precipitation centrifuge to obtain upper liquid phase B and precipitate B. Liquid phase A and liquid phase B are mixed, concentrated under reduced pressure at 40 ° C. to remove n-hexane, and then krill oil is obtained. The recovered n-hexane can be used in duplicate. Precipitation A and Precipitation B are mixed and 0.6 times the volume of water is added. Then, it is spray-dried under conditions of a drying tower intake temperature of 160 ° C. and an exhaust temperature of 85 ° C. to produce a dry meal. , Transported back to the land and used as a raw material to extract proteins and other nutrients.

  Example 6: Onboard processing method for producing krill oil from Nankoku krill, comprising the following steps.

  The freshly collected Antarctic krill is drained and then homogenized directly. The homogenate solution is irradiated with UV light at a power of 34 watts, a wavelength of 230 nm and a distance of 0.5 m for 30 minutes, and the temperature is 0 to 4 ° C. (capture season Antarctica Self-digest at low temperature for 6 hours at the average environmental temperature of the district. Each of the homogenates after low-temperature self-digestion treatment is 0.5 times the volume of water, NaCl (final concentration 2.0-2.5%) and ethyl alcohol (final concentration 0.05-0.1%). The mixture is uniformly mixed, and the homogenate liquid is treated by irradiating ultraviolet rays with a power of 40 watts, a wavelength of 208 nm and a distance of 0.5 m for 40 minutes, and the pH value of the homogenate liquid is 7.5 to 8 with 1 M HCl or NaOH. The temperature is increased to 65 ° C. at a rate of 3 ° C. every 10 minutes, and high temperature self-digestion is performed for 5 hours under these conditions.

Add 1 volume of water to the homogenate after high temperature autolysis, mix evenly, treat with 800 watts of ultrasonic power (367 nm) for 28 minutes, and then adjust the pH of the homogenate with 1M HCl or NaOH. The value is adjusted to 7.5-8.0, alkaline protease (9 × 10 ⁵ U / kg substrate) is added to the homogenate solution, mixed uniformly, and enzymatically degraded at 56 ° C. for 4 hours.

  1-fold volume of n-hexane is added to the enzyme decomposition solution, mixed uniformly, allowed to stand for 90 minutes, and centrifuged with a precipitation centrifuge to obtain upper liquid phase A and precipitate A. 1-fold volume of n-hexane is added to precipitate A, mixed uniformly, allowed to stand for 40 minutes, and centrifuged with a precipitation centrifuge to obtain upper liquid phase B and precipitate B. Liquid phase A and liquid phase B are mixed, concentrated under reduced pressure at 40 ° C. to remove n-hexane, and then krill oil is obtained. The recovered n-hexane can be used in duplicate. Precipitation A and Precipitation B are mixed and 0.6 times the volume of water is added. Then, it is spray-dried under conditions of a drying tower intake temperature of 160 ° C. and an exhaust temperature of 85 ° C. to produce a dry meal. , Transported back to the land and used as a raw material to extract proteins and other nutrients.

  Example 7: A method for producing krill phospholipids from Antarctic krill comprising the following steps:

  1. Raw material treatment: Freshly harvested Antarctic krill is drained and then homogenized directly.

  2. Low temperature self-digestion: Treated Antarctic krill homogenate with UV light of 40 watts, wavelength 300 nm, distance 0.5 m for 10-30 minutes, treated homogenate at 0-4 ° C (capture season Antarctica) 2) hours of self-digestion.

  The Antarctic krill homogenate solution after self-digestion treatment is directly frozen and stored. (The above operation steps are all completed on board, and the advantage is that self-digestion ability of fresh Antarctic krill is strong, increasing self-digestion of structural proteins and improving phospholipid yield. That is true.)

Exogenous enzymatic degradation assisted by ultrasound: Thawing the cryogenic-preserved Antarctic krill homogenate, then adding 1 to 3 times the volume of water to the cryopreserved Antarctic krill homogenate Treated with 300-800 watts of ultrasound (389 nm) for 10 minutes, adjusted to pH 7.5-8.0 with 1M HCl or NaOH, and alkaline protease (9-10 × 10 ⁵ U in homogenate). / Kg substrate) is added, mixed uniformly, and enzymatically degraded at 60 ° C. for 1 hour.

  Extraction of Antarctic krill phospholipid: The sample after enzymatic degradation with exogenous enzyme is spray-dried to obtain a dry meal. Add 5-15 times volume of 95% ethyl alcohol to dry meal of Antarctic krill, mix uniformly, let stand for 30-90 minutes, centrifuge in sedimentation centrifuge, upper liquid phase A and precipitate Get A. Add 2.5-7.5 times volume of ethyl alcohol to Precipitate A, mix evenly, let stand for 20-60 minutes, and centrifuge in Precipitation Centrifuge to separate Upper Liquid Phase B and Precipitate B obtain. Liquid phase A and liquid phase B are mixed and concentrated under reduced pressure at 40 ° C. to remove ethyl alcohol to obtain Antarctic krill phospholipids. The recovered ethyl alcohol can be reused.

  Example 8: A method for producing krill phospholipids from Antarctic krill comprising the following steps:

  1. Ingredients and processing: Freshly captured Antarctic krill is drained and then homogenized directly.

  2. Low-temperature self-digestion: Treated Antarctic krill homogenate with UV power of 40 watts, wavelength 200-300 nm, distance 0.5 m for 10-30 minutes, and placed the treated homogenate at 0-4 ° C. Self-digest for 2-10 hours.

  After spray-drying the homogenate solution of Antarctic krill after the self-digestion treatment, a dry meal is obtained and stored. (The above operation steps are all completed on board, and the advantage is that self-digestion ability of fresh Antarctic krill is strong, increasing self-digestion of structural proteins and improving phospholipid yield. That is true.)

Exogenous enzymatic degradation assisted by ultrasound: the dry meal is dissolved in 7-12 volumes of water, mixed homogeneously, treated with 300-800 watts of ultrasound (200-400 nm) for 10 minutes, 1M HCl Alternatively, the pH is adjusted to 7.5 to 8.0 with NaOH, alkaline protease (9 to 10 × 10 ⁵ U / kg substrate) is added to the homogenate solution, mixed uniformly, and enzymatically degraded at 60 ° C. for 1 hour. .

  Extraction of Antarctic krill phospholipid: The sample after enzymatic degradation with exogenous enzyme is spray-dried to obtain a dry meal. Add 5-15 times volume of 95% ethyl alcohol to dry meal of Antarctic krill, mix uniformly, let stand for 30-90 minutes, centrifuge in sedimentation centrifuge, upper liquid phase A and precipitate Get A. Add 2.5-7.5 times volume of ethyl alcohol to Precipitate A, mix evenly, let stand for 20-60 minutes, and centrifuge in Precipitation Centrifuge to separate Upper Liquid Phase B and Precipitate B obtain. Liquid phase A and liquid phase B are mixed and concentrated under reduced pressure at 40 ° C. to remove ethyl alcohol to obtain Antarctic krill phospholipids. The recovered ethyl alcohol can be reused.

  Example 9: A method for producing krill phospholipids from Antarctic krill comprising the following steps:

  1. Raw material treatment: Freshly harvested Antarctic krill is drained and then homogenized directly.

  2. Low-temperature self-digestion: A processing process is carried out by irradiating the Antarctic krill homogenate solution with ultraviolet rays having a power of 40 watts, a wavelength of 205 nm and a distance of 0.5 m for 10 minutes, and the treated homogenate solution is self-digested at a capture environment temperature for 2 hours.

3. Exogenous enzymatic degradation assisted by ultrasound: add 1 volume of water to homogenate, treat with 300 watts ultrasound (350 nm) for 10 minutes, adjust pH to 7.5 with 1M HCl or NaOH. Then, alkaline protease (9 × 10 ⁵ U / kg) is added to the homogenate solution, mixed uniformly, and enzymatically degraded at 60 ° C. for 1 hour. The sample after enzymatic degradation with exogenous enzyme is spray-dried to obtain a dry meal.

  4). Extraction of Antarctic krill phospholipids: Add 5 volumes of 95% ethyl alcohol to dry meal, mix uniformly, let stand for 30 minutes, centrifuge in sedimentation centrifuge, Precipitate A is obtained. 2.5 times volume of ethyl alcohol is added to the precipitate A, mixed uniformly, allowed to stand for 20 minutes, and centrifuged with a precipitation centrifuge to obtain an upper liquid phase B and a precipitate B. Liquid phase A and liquid phase B are mixed and concentrated under reduced pressure at 40 ° C. to remove ethyl alcohol to obtain Antarctic krill phospholipids.

  Example 10: A method for producing krill phospholipids from Antarctic krill, comprising the following steps.

  1. Raw material treatment: Freshly harvested Antarctic krill is drained and then homogenized directly.

  2. Low-temperature self-digestion: Treated Antarctic krill homogenate with ultraviolet light of power 40 watts, wavelength 210 nm, distance 0.5 m for 20 minutes and placed the treated homogenate at 0-4 ° C for 5 hours To do.

3. Exogenous enzymatic degradation assisted by ultrasound: Add 1 to 3 volumes of water to the homogenate solution, treat with 300-800 watts ultrasound (380 nm) for 10 minutes, and adjust pH with 4M HCl or NaOH to 7. Adjust to 5 to 8.0, add alkaline protease (9 × 10 ⁵ U / kg substrate) to the homogenate solution, mix uniformly, and perform enzymatic degradation at 56 ° C. for 1 hour. The sample after enzymatic degradation is spray-dried to obtain a dry meal.

  4). Extraction of Antarctic krill phospholipids: Add 5-15 volumes of 95% ethyl alcohol to dry meal, mix uniformly, let stand for 30-90 minutes, centrifuge in sedimentation centrifuge, top layer Liquid phase A and precipitate A are obtained. Liquid phase A is concentrated under reduced pressure at 41 ° C. to remove ethyl alcohol to obtain Antarctic krill phospholipids. The recovered ethyl alcohol can be reused.

  Example 11: A method for producing krill phospholipids from Antarctic krill comprising the following steps:

  1. Raw material treatment: Freshly harvested Antarctic krill is drained and then homogenized directly.

  2. Low-temperature self-digestion: Treated Antarctic krill homogenate with UV light of 40 watts, wavelength 240 nm, distance 0.8 m for 10-30 minutes, and treated homogenate at 0-4 ° C for 6 hours To do.

  3. The Antarctic krill homogenate solution after self-digestion treatment is directly frozen and stored at -15 to -25 ° C.

4). Exogenous enzymatic digestion assisted by ultrasound: Thawing a cryogenically preserved Antarctic krill homogenate, adding 2 volumes of water, treating with 550 watts ultrasound (250 nm) for 10 minutes, 1M Adjust the pH to 7.75 with HCl or NaOH, add alkaline protease (9.5 × 10 ⁵ U / kg substrate) to the homogenate solution, mix uniformly, and perform enzymatic degradation at 60 ° C. for 3 hours. The sample after enzymatic degradation with an exogenous enzyme is spray-dried to obtain a dry meal.

  5. Extraction of Antarctic krill phospholipid: Add 10 volumes of 95% ethyl alcohol to dry meal of Antarctic krill, mix uniformly, let stand for 60 minutes, centrifuge in sedimentation centrifuge, upper layer Liquid phase A and precipitate A are obtained. 5 times volume of ethyl alcohol is added to the precipitate A, mixed uniformly, allowed to stand for 40 minutes, and centrifuged with a precipitation centrifuge to obtain an upper liquid phase B and a precipitate B. Liquid phase A and liquid phase B are mixed and concentrated under reduced pressure at 48 ° C. to remove ethyl alcohol to obtain Antarctic krill phospholipids. The recovered ethyl alcohol can be reused.

  In a preferred form, Steps 1 to 3 above are completed on a fishing boat, and the merit is that the self-digestion ability of fresh Antarctic krill is strong, increasing self-digestion of structural proteins, and phospholipids. This is to improve the yield. The next step may be completed by transporting the cryogenized homogenate solution back to land, or may be operated on board as needed.

  Example 12: A method for producing krill phospholipids from Antarctic krill, comprising the following steps:

  1. Raw material treatment: Freshly harvested Antarctic krill is drained and then homogenized directly.

  2. Low-temperature self-digestion: Treated Antarctic krill homogenate with UV light of power 40 watts, wavelength 255 nm, distance 0.9 m for 10 to 30 minutes, and processed homogenate for 2 to 10 hours at capture environment temperature Digest.

  3. The Antarctic krill homogenate solution after self-digestion treatment is directly frozen and stored at -15 to -25 ° C.

4). Exogenous enzymatic digestion assisted by ultrasound: after thawing the cryopreserved Antarctic krill homogenate, add 1-3 volumes of water and 10 minutes with 300-800 Watts ultrasound (400 nm) Treat, adjust pH to 7.5-8.0 with 1M HCl or NaOH, add alkaline protease (9-10 × 10 ⁵ U / kg substrate) to homogenate, mix uniformly, 61 ° C. Enzymatically digest for 3 hours. The sample after enzymatic degradation with an exogenous enzyme is spray-dried to obtain a dry meal.

  5. Extraction of Antarctic krill phospholipid: Add 5-15 times volume of 95% ethyl alcohol to dry meal of Antarctic krill, mix uniformly, let stand for 30-90 minutes, centrifuge in sedimentation centrifuge Thus, the upper liquid phase A and the precipitate A are obtained. Liquid phase A is concentrated under reduced pressure at 52 ° C. to remove ethyl alcohol to obtain Antarctic krill phospholipids. The recovered ethyl alcohol can be reused.

  In a preferred form, steps 1-3 above are completed on a fishing boat, and the merit is that the self-digestion ability of fresh Antarctic krill is strong, increasing self-digestion of structural proteins, It is to improve the yield. The next step may be completed by transporting the cryogenized homogenate solution back to land, or may be operated on board as needed.

  Example 13: A method for producing krill phospholipids from Antarctic krill, comprising the following steps.

  1. Raw material treatment: Freshly harvested Antarctic krill is drained and then homogenized directly.

  2. Low temperature self-digestion: Antarctic krill homogenate liquid is treated by irradiating with UV light of power 40 watts, wavelength 235 nm, distance 0.5 m for 25 minutes, and the treated homogenate liquid self-digests at capture environment temperature for 8.5 hours .

  3. The Antarctic krill homogenate solution after self-digestion treatment is directly frozen and stored at -15 to -25 ° C.

4). Exogenous enzymatic digestion assisted by ultrasound: Thawed Antarctic krill homogenate that had been cryopreserved, added 2.5 volumes of water, and treated with 650 watts of ultrasound (345 nm) for 10 minutes. The pH is adjusted to 7.9 with 1M HCl or NaOH, alkaline protease (10 × 10 ⁵ U / kg substrate) is added to the homogenate solution, mixed uniformly, and enzymatically degraded at 62 ° C. for 4 hours. The sample after enzymatic degradation with an exogenous enzyme is spray-dried to obtain a dry meal.

  5. Extraction of Antarctic krill phospholipid: Add 12.5 times volume of 95% ethyl alcohol to dry meal of Antarctic krill, mix uniformly, let stand for 75 minutes, centrifuge in sedimentation centrifuge. The upper liquid phase A and the precipitate A are obtained. Add 6.5 volumes of ethyl alcohol to the precipitate A, mix uniformly, let stand for 45 minutes, and centrifuge in a precipitation centrifuge to obtain the upper liquid phase B and the precipitate B. Liquid phase A and liquid phase B are mixed and concentrated under reduced pressure at 55 ° C. to remove ethyl alcohol to obtain Antarctic krill phospholipids. The recovered ethyl alcohol can be reused.

  In a preferred form, Steps 1 to 3 above are completed on a fishing boat, and the merit is that the self-digestion ability of fresh Antarctic krill is strong, increasing self-digestion of structural proteins, and phospholipids. This is to improve the yield. The next step may be completed by transporting the cryogenized homogenate solution back to land, or may be operated on board as needed.

  Example 14: A method for producing krill phospholipids from Antarctic krill comprising the following steps:

  1. Ingredients and processing: Freshly captured Antarctic krill is drained and then homogenized directly.

  2. Low temperature self-digestion: Antarctic krill homogenate is treated by irradiating with UV light of power 40 watts, wavelength 200 nm, distance 0.5 m for 25 minutes, and the treated homogenate is self-digested for 8 hours at the capture environment temperature.

  3. A dry meal is obtained after spray drying the homogenate solution and stored at -15 to -25 ° C.

4). Exogenous enzymatic digestion assisted by ultrasound: The dried meal of Antarctic krill is dissolved in 11 volumes of water, mixed uniformly, treated with 700 watts of ultrasound (300 nm) for 10 minutes, 1 M HCl or NaOH PH is adjusted to 7.9, and alkaline protease (10 × 10 ⁵ U / kg substrate) is added to the homogenate solution, mixed uniformly, and enzymatically degraded at 60 ° C. for 1 hour. The sample after enzymatic degradation with an exogenous enzyme is spray-dried to obtain a dry meal.

  5. Extraction of Antarctic krill phospholipids: Add 14 volumes of 95% ethyl alcohol to dry meal of Antarctic krill, mix uniformly, let stand for 85 minutes, centrifuge in sedimentation centrifuge, top layer Liquid phase A and precipitate A are obtained. 7 times volume of ethyl alcohol is added to the precipitate A, mixed uniformly, allowed to stand for 55 minutes, and centrifuged with a precipitation centrifuge to obtain an upper liquid phase B and a precipitate B. Liquid phase A and liquid phase B are mixed and concentrated under reduced pressure at 40 ° C. to remove ethyl alcohol to obtain Antarctic krill phospholipids. The recovered ethyl alcohol can be reused.

  In a preferred form, Step 1 to Step 3 are completed on a fishing boat, and the merit is that the self-digestion ability of fresh Antarctic krill is strong, increasing the self-digestion of structural proteins, and phospholipids. This is to improve the yield. The next step may be completed by transporting the dry meal that has been cryopreserved back to land, or may be operated on board as needed.

  Example 15: Onboard high speed production method of Antarctic krill concentrate and meal comprising the following steps.

  1. Raw material treatment: Freshly caught Antarctic krill is drained and then crushed by pressing to obtain crushed material.

  2. Low-temperature self-digestion: After irradiating the crushed material with ultraviolet rays having a power of 20 to 40 watts, a wavelength of 200 to 300 nm, and a distance of 0.5 to 1 m for 10 to 30 minutes, 0 to 4 ° C. (environment temperature of the capture area) 1 to 5 Self-digest time.

3. Low temperature exogenous enzymatic degradation assisted by ultrasound: 1 to 3 volumes of water is added to the crushed material, treated with 300 to 800 watts of ultrasound (wavelength 200 to 400 nm) for 5 to 15 minutes, 1M HCl Alternatively, the pH value of the crushed suspension is adjusted to 7.5 to 8.5 with NaOH, and 9 to 10 × 10 ⁵ U / kg substrate low-temperature protease is added to the homogenate solution, and the pH is 10 to 20 ° C. Enzymatic degradation is performed for 1 to 4 hours to obtain an enzymatic degradation product. The low temperature protease selects Novozymes Polarzyme.

  4). The enzymatic degradation product passes through a 120-200 mesh sieve, and the filtrate is concentrated at low temperature under vacuum to obtain a peanut krill concentrate or spray dried to obtain a peanut krill dry meal.

Example 16: On-board high speed production method of Antarctic krill concentrate and meal, the steps are as follows. The fresh Antarctic krill is drained and then crushed by press. The crushed material is irradiated with ultraviolet rays of 20 watts, a wavelength of 232 nm and a distance of 0.5 m for 10 minutes, and then self-digests for 1 hour at the environmental temperature in the capture area. After adding 1 volume of water to the slurry after low-temperature self-digestion and treating with ultrasonic waves (345 nm) of 300 watts for 5 minutes, the pH value of the crushed suspension is set to 7 with 1 M HCl or NaOH. 5 × 9 × 10 ⁵ U / kg substrate of low temperature protease is added to the homogenate solution and enzymatically degraded at 10 ° C. for 1 hour. The enzyme degradation product passed through a 120-mesh sieve, and the filtrate was concentrated in vacuo at a concentration temperature of 20 to 25 ° C. and a degree of vacuum of 75 to 90 kPa to produce a concentrate.

Example 17: On-board high-speed production method of Antarctic krill concentrate and meal, the steps are as follows. The fresh Antarctic krill is drained and then crushed by press. The crushed material is irradiated with ultraviolet rays of 20 watts, a wavelength of 245 nm and a distance of 1 m for 10 minutes, and then self-digests for 3 hours at the environmental temperature of the capture area. After adding 1 volume of water to the slurry after low temperature self-digestion and treating it with 500 watts of ultrasonic waves (265 nm) for 10 minutes, the pH value of the crushed suspension is adjusted to 8 with 1 M HCl or NaOH. The temperature is adjusted to 0, and 10 ⁶ U / kg substrate low-temperature protease is added to the homogenate solution, followed by enzymatic degradation at 10 ° C. for 3 hours. The enzyme degradation product passed through a 160-mesh sieve, and the filtrate was concentrated in vacuo at a concentration temperature of 25 ° C. and a degree of vacuum of 75 kPa to produce a concentrate.

Example 18: Onboard high-speed production method of Antarctic krill concentrate and meal, the steps are as follows. The fresh Antarctic krill is drained and then crushed by press. The crushed material is irradiated with ultraviolet rays of 20 watts, a wavelength of 255 nm and a distance of 0.75 m for 20 minutes, and then self-digests for 5 hours at the environmental temperature of the capture area. Three times the volume of water was added to the slurry after low temperature self-digestion, treated with 500 watts of ultrasonic waves (270 nm) for 10 minutes, and then the pH value of the crushed suspension was adjusted to 8. with 1 M HCl or NaOH. The temperature is adjusted to 0, and 9 × 10 ⁵ U / kg substrate low-temperature protease is added to the homogenate solution, followed by enzymatic degradation at 20 ° C. for 1 hour. The enzyme degradation product passed through a 160-mesh sieve, and the filtrate was concentrated in vacuo at a concentration temperature of 20 ° C. and a vacuum degree of 80 kPa to produce a concentrate.

Example 19: On-board high-speed production method of Antarctic krill concentrate and meal, the steps are as follows. The fresh Antarctic krill is drained and then crushed by press. The crushed material is irradiated with ultraviolet rays of 20 watts, a wavelength of 270 nm and a distance of 1 m for 30 minutes, and then self-digests for 5 hours at the environmental temperature of the capture area. After adding 2 volumes of water to the slurry after low-temperature self-digestion and treating it with 800 watts of ultrasonic waves (365 nm) for 10 minutes, the pH value of the crushed suspension is set to 7 with 1 M HCl or NaOH. 5 and a low temperature protease of 10 ⁶ U / kg substrate is added to the homogenate solution, followed by enzymatic degradation at 10 ° C. for 4 hours. The enzyme degradation product passed through a 200-mesh sieve, and the filtrate was vacuum concentrated at a concentration temperature of 25 ° C. and a vacuum degree of 85 kPa to produce a concentrate.

Example 20: A high-speed on-board production method of Antarctic krill concentrate and meal, the steps being as follows. The fresh Antarctic krill is drained and then crushed by press. The crushed material is irradiated with ultraviolet rays of 20 watts, a wavelength of 278 nm, and a distance of 1 m for 30 minutes, and then self-digests at 4 ° C. (environmental temperature in the capture area) for 5 hours. Three times the volume of water was added to the slurry after low temperature self-digestion, treated with 800 watts of ultrasonic waves (378 nm) for 15 minutes, and then the pH value of the crushed suspension was adjusted to 8. with 1 M HCl or NaOH. 5 × 9 × 10 ⁵ U / kg substrate low temperature protease is added to the homogenate solution and enzymatically degraded at 20 ° C. for 4 hours. The enzyme degradation product passed through a 200-mesh sieve, and the filtrate was vacuum concentrated at a concentration temperature of 25 ° C. and a vacuum degree of 90 kPa to produce a concentrate.

Example 21: Onboard high speed production method of Antarctic krill concentrate and meal, the steps are as follows. The fresh Antarctic krill is drained and then crushed by press. The crushed material is irradiated with ultraviolet rays of 3 watts, a wavelength of 290 nm, and a distance of 0.5 m for 10 minutes, and then self-digests for 1 hour at the environmental temperature in the capture area. After adding 1 volume of water to the slurry after low-temperature self-digestion and treating with ultrasonic waves (389 nm) of 300 watts for 5 minutes, the pH value of the crushed suspension is set to 7 with 1 M HCl or NaOH. 5 × 9 × 10 ⁵ U / kg substrate of low temperature protease is added to the homogenate solution and enzymatically degraded at 10 ° C. for 1 hour. The enzyme degradation product passed through a 120-mesh sieve, and the filtrate was vacuum concentrated at a concentration temperature of 20 ° C. and a degree of vacuum of 75 kPa to produce a concentrate.

Example 22: Onboard high-speed production method of Antarctic krill concentrate and meal, the steps are as follows. The fresh Antarctic krill is drained and then crushed by press. The crushed material is irradiated with ultraviolet rays of 40 watts, a wavelength of 295 nm and a distance of 1 m for 30 minutes, and then self-digests for 5 hours at the environmental temperature of the capture area. After adding 2 volumes of water to the slurry after low-temperature self-digestion and treating with 800 watts of ultrasonic waves (498 nm) for 10 minutes, the pH value of the crushed suspension is adjusted to 7M with 1M HCl or NaOH. 5 and a low temperature protease of 10 ⁶ U / kg substrate is added to the homogenate solution, followed by enzymatic degradation at 10 ° C. for 4 hours. The enzyme degradation product passed through a 200-mesh sieve, and the filtrate was spray-dried at a drying tower intake temperature of 150 to 180 ° C. and an exhaust temperature of 75 to 95 ° C. to produce a dry meal.

Example 23: Onboard high-speed production method of Antarctic krill concentrate and meal, the steps being as follows. The fresh Antarctic krill is drained and then crushed by press. The crushed material is irradiated with ultraviolet rays of 40 watts, a wavelength of 300 nm and a distance of 1 m for 30 minutes, and then self-digests for 5 hours at the environmental temperature of the capture area. Three times the volume of water was added to the slurry after low temperature self-digestion, treated with 800 watts of ultrasonic waves (400 nm) for 15 minutes, and then the pH value of the crushed suspension was adjusted to 8. with 1 M HCl or NaOH. 5 × 9 × 10 ⁵ U / kg substrate low temperature protease is added to the homogenate solution and enzymatically degraded at 20 ° C. for 4 hours. The enzyme degradation product passed through a 200-mesh sieve, and the filtrate was spray-dried at a drying tower intake temperature of 180 ° C. and an exhaust temperature of 95 ° C. to produce a dry meal.

The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited to this, and the technical scope of the present invention is within the technical scope disclosed by the person skilled in the art. Those obtained by equivalent substitution or modification based on the means and the inventive concept are included in the protection scope of the present invention.

Claims (4)

A method for producing krill oil from Nankoku krill,
S1. A raw material processing step of draining the captured Antarctic krill and then homogenate or press crushing to obtain a krill raw material,
S2. A self-digestion step of irradiating the krill raw material with ultraviolet rays and then self-digesting at 0 to 4 ° C. to obtain a self-digestion solution;
S3. A sonication step of treating the autolysate after hydration with ultrasound;
S4. The pH value of the autolysate is adjusted to 7.5 to 8.0, an alkaline protease is added to the autolysate, and enzymatic degradation is performed at 55 to 65 ° C. to obtain an enzyme digestion solution. An enzymatic degradation step;
S5. Extracting krill oil from the enzymatic decomposition solution using ethyl alcohol or n-hexane;
A method for producing krill oil from Antarctic krill, comprising: A method for producing krill oil from the Antarctic krill according to claim 1,
A method for producing krill oil, wherein the alkaline protease is a Bacillus subtilis alkaline protease. There is provided a method of manufacturing the Antarctic krill or Lao Kiamimiru,
S1. A raw material processing step of draining the captured Antarctic krill and then homogenate or press crushing to obtain a krill raw material,
S2. After irradiating the krill raw material with ultraviolet rays, self-digestion at 0 to 4 ° C. to obtain a self-digestion solution,
S3. A sonication step of treating the autolysate after hydration with ultrasound;
S4. The pH value of the autolysate is adjusted to 7.5 to 8.5, 9 to 10 × 10 ⁵ U / kg substrate of low temperature protease is added to the autolysate, and the mixture is heated at 10 to 20 ° C. for 1 to 4 hours. Enzymatic degradation step with an exogenous enzyme to obtain an enzymatic degradation solution by enzymatic degradation,
S5. To obtain a filtrate is passed through the enzyme hydrolyzate was a sieve of 120 to 200 mesh, for the filtrate, obtaining a krill dried meal after mists dried,
Features and to Luo Kiamimiru method for manufacturing comprises a. A method for producing a krill concentrate from a southern krill,
S1. A raw material processing step of draining the captured Antarctic krill and then homogenate or press crushing to obtain a krill raw material,
S2. After irradiating the krill raw material with ultraviolet rays, self-digestion at 0 to 4 ° C. to obtain a self-digestion solution,
S3. A sonication step of treating the autolysate after hydration with ultrasound;
S4. The pH value of the autolysate is adjusted to 7.5 to 8.5, 9 to 10 × 10 ⁵ U / kg substrate of low temperature protease is added to the autolysate, and the mixture is heated at 10 to 20 ° C. for 1 to 4 hours. Enzymatic degradation step with an exogenous enzyme to obtain an enzymatic degradation solution by enzymatic degradation,
S5. To obtain a filtrate is passed through the enzyme hydrolyzate was a sieve of 120 to 200 mesh, for the filtrate, Luz step to obtain a krill concentrate solids after cold concentrated in vacuo occupies 40% to 70% When,
A method for producing a krill concentrate, comprising: