CN103141872A - Method for preparing active substance by sea cucumber intestine autolysis - Google Patents
Method for preparing active substance by sea cucumber intestine autolysis Download PDFInfo
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- CN103141872A CN103141872A CN2011104357118A CN201110435711A CN103141872A CN 103141872 A CN103141872 A CN 103141872A CN 2011104357118 A CN2011104357118 A CN 2011104357118A CN 201110435711 A CN201110435711 A CN 201110435711A CN 103141872 A CN103141872 A CN 103141872A
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- 241000251511 Holothuroidea Species 0.000 title claims abstract description 145
- 210000000936 intestine Anatomy 0.000 title claims abstract description 140
- 238000000034 method Methods 0.000 title claims abstract description 28
- 208000035404 Autolysis Diseases 0.000 title abstract 4
- 206010057248 Cell death Diseases 0.000 title abstract 4
- 239000013543 active substance Substances 0.000 title abstract 4
- 230000028043 self proteolysis Effects 0.000 title abstract 4
- 238000004108 freeze drying Methods 0.000 claims abstract description 43
- 229920005654 Sephadex Polymers 0.000 claims abstract description 36
- 239000012507 Sephadex™ Substances 0.000 claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 102000004190 Enzymes Human genes 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 19
- 238000000926 separation method Methods 0.000 claims abstract description 6
- 239000011149 active material Substances 0.000 claims description 91
- 239000012043 crude product Substances 0.000 claims description 36
- 239000006228 supernatant Substances 0.000 claims description 36
- 239000008367 deionised water Substances 0.000 claims description 25
- 229910021641 deionized water Inorganic materials 0.000 claims description 25
- 238000010438 heat treatment Methods 0.000 claims description 22
- 239000002002 slurry Substances 0.000 claims description 15
- 238000001816 cooling Methods 0.000 claims description 14
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 11
- 238000000108 ultra-filtration Methods 0.000 claims description 9
- 229910001220 stainless steel Inorganic materials 0.000 claims description 8
- 239000010935 stainless steel Substances 0.000 claims description 8
- 239000008055 phosphate buffer solution Substances 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 4
- 238000007670 refining Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 abstract description 15
- 239000000463 material Substances 0.000 abstract description 13
- 239000000796 flavoring agent Substances 0.000 abstract description 10
- 235000019634 flavors Nutrition 0.000 abstract description 10
- 239000000843 powder Substances 0.000 abstract description 10
- 239000007787 solid Substances 0.000 abstract description 10
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 230000003078 antioxidant effect Effects 0.000 abstract 1
- 230000002358 autolytic effect Effects 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 235000013376 functional food Nutrition 0.000 abstract 1
- 230000006698 induction Effects 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 239000000047 product Substances 0.000 description 18
- 238000005057 refrigeration Methods 0.000 description 12
- CBMPTFJVXNIWHP-UHFFFAOYSA-L disodium;hydrogen phosphate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].OP([O-])([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O CBMPTFJVXNIWHP-UHFFFAOYSA-L 0.000 description 10
- 230000003064 anti-oxidating effect Effects 0.000 description 9
- 239000012141 concentrate Substances 0.000 description 9
- 235000013305 food Nutrition 0.000 description 9
- 238000000338 in vitro Methods 0.000 description 9
- 230000009965 odorless effect Effects 0.000 description 9
- 230000001105 regulatory effect Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 6
- 244000063299 Bacillus subtilis Species 0.000 description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 description 5
- 230000001276 controlling effect Effects 0.000 description 4
- 238000003754 machining Methods 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 2
- 238000010612 desalination reaction Methods 0.000 description 2
- -1 hydrazine free radical Chemical class 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OTEKOJQFKOIXMU-UHFFFAOYSA-N 1,4-bis(trichloromethyl)benzene Chemical compound ClC(Cl)(Cl)C1=CC=C(C(Cl)(Cl)Cl)C=C1 OTEKOJQFKOIXMU-UHFFFAOYSA-N 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000003412 degenerative effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000001641 gel filtration chromatography Methods 0.000 description 1
- OAKJQQAXSVQMHS-UHFFFAOYSA-N hydrazine Substances NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 1
- 230000002706 hydrostatic effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/50—Molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Marine Sciences & Fisheries (AREA)
- Mycology (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for preparing an active substance by sea cucumber intestine autolysis. The method comprises the following steps of homogenizing sea cucumber intestine, carrying out ultraviolet irradiation induction so that the sea cucumber intestine autolyzes in the presence of an endogenous enzyme under a certain condition, and carrying out centrifugation, freeze concentration, Sephadex G-15 separation and freeze drying of the autolytic mixed liquid. The active substance prepared by sea cucumber intestine autolysis is faint yellow solid powder, has no odour, has a delicate flavor of sea cucumber, can be dissolved in water easily, has hygroscopicity and a certain external antioxidant activity, and can be used as a functional base material for preparation of multiple functional foods. The method utilizes a sea cucumber intestine autolysis capacity to realize preparation of the active substance, retains the original nutrients to the maximum degree, reduces environmental pollution, has reasonable and simple processes and a low cost, can prepare the green product and does not produce pollution.
Description
Technical field
The invention belongs to marine byproduct intensive processing field, relate to simultaneously the technical field of self-dissolving, gel filtration chromatography and vacuum freeze drying.
Background technology
Sea cucumber intestine is the accessory substance in the Holothurian machining process, and its protein content is high, can reach 70% left and right (with dry weight basis), is the very good material of preparation active material.In China, sea cucumber intestine is used as discarded object more and abandons, and utilization rate is extremely low, causes the wasting of resources and environmental pollution.
Sea cucumber intestine has stronger self-dissolving ability.At present, the relevant Patents that utilizes the self-dissolving technology to prepare active material from sea cucumber intestine has no report.About the bibliographical information of sea cucumber intestine self-dissolving, mainly concentrate on to its endogenous enzymes separate, the research of purifying and enzymatic property.Japanese scholars improves the preparation method of japanese traditional fermented food " salt is hot ", adopts hydrostatic pressing not add salt and promote the sea cucumber intestine self-dissolving, but does not relate to the extraction of sea cucumber intestine self-dissolving active material and separate preparation.
Summary of the invention
Stronger for the accessory substance sea cucumber intestine self-dissolving ability in Holothurian machining, but simultaneously and the situation that is underutilized, the present invention aims to provide a kind of preparation method who utilizes the self-dissolving technology to prepare sea cucumber intestine active material and self-dissolving residue active material thereof.
The present invention utilizes the sea cucumber intestine self-dissolving to prepare the method for active material, take sea cucumber intestine as raw material, is the sea cucumber intestine autolysate through homogenate, self-dissolving, centrifuging and taking supernatant; This product can further adopt the method for freeze concentration to be concentrated into 10%～20% of original volume; Can also adopt the mode of vacuum freeze drying to be dried to moisture less than or equal to 8%, thereby obtain the sea cucumber intestine active material crude product of different product form.
Wherein, the preferred version of described self-dissolving operation is: after homogenate, the gained homogenate after the ultraviolet ray irradiation, adds citric acid and the disodium hydrogen phosphate buffer solution of 1～4 times of volume, pH4.0～7.0, carries out self-dissolving.Self-dissolvings different from actual conditions can be taked two kinds of conditions: temperature in the time of 4～10 ℃, self-dissolving 4～12h, perhaps temperature when 35 ℃～60 ℃ of self-dissolvings, self-dissolving 0.5～4h.
Under optimal way, when treating capacity was less, ultraviolet irradiation can be selected the 30W uviol lamp, induced apart from carrying out self-dissolving after 0.5m irradiation 5～40min; When treating capacity is larger, the sea cucumber intestine homogenate can be placed in stainless steel tank, slurries thickness 1～2cm, 0.2～0.5m places uviol lamp in the place up, and controlling slurries liquid level UV intensity is 60～120 μ W/cm
2, keep 5～40min to carry out self-dissolving and induce.
In addition, the operation of described centrifuging and taking supernatant, optimal way is: the centrifugal 10～30min of 2500～6000r/min, get supernatant; Or the mode of employing ultrafiltration, collect the following component of 10kDa.
In order to obtain sea cucumber intestine active material highly finished product, the present invention makes refining active material through separation, freeze drying after making sea cucumber intestine active material crude product.
Wherein, the operation of described separation, optimal way is: described sea cucumber intestine active material crude product is splined on sephadex Sephadex G-15 chromatographic column, use the deionized water wash-out, coutroi velocity is 0.3～0.5mL/min, and key component (peak area account for each component total peak area 70%～80%) is collected.Adopt hexichol to measure system for bitter taste hydrazine free radical (DPPH) system and reducing power, proved that key component has stronger removing DPPH free radical and reducing power.
In addition, described cryodesiccated preferable process is: in condenser temperature-30～-45 ℃, vacuum is to carry out drying under 50～80Pa condition.
The present invention provides a kind of method of utilizing the sea cucumber intestine self-dissolving to prepare active material in addition, and mainly for sea cucumber intestine self-dissolving residue active material, its purpose is to improve the utilization rate of sea cucumber intestine.Detailed process is, take sea cucumber intestine as raw material, through homogenate, self-dissolving.Then separation of supernatant obtains the sea cucumber intestine autolysate, utilizes simultaneously remaining residue to be handled as follows: add the deionized water mixing of 2～4 times of volumes, 90～100 ℃ of heating 5～15min.In 50 ℃, under pH7.0, add the neutral proteinase of 1500～3000U/g albumen after cooling, as bacillus subtilis 1398, enzymolysis 30～180min.After enzymolysis was complete, enzymolysis liquid was in 95～100 ℃ of heating 5～10min enzymes that go out, cooling after under 2500～6000r/min centrifugal 10～30min, get supernatant, or adopt the mode of ultrafiltration, collect the following component of 10kDa, be sea cucumber intestine self-dissolving residue active material.This product can further adopt the method for freeze concentration to be concentrated into 10%～20% of original volume; Can also adopt the mode of vacuum freeze drying to be dried to moisture less than or equal to 8%, thereby obtain the sea cucumber intestine self-dissolving residue active material crude product of different product form.
Described sea cucumber intestine self-dissolving residue active material crude product is splined on sephadex Sephadex G-25 chromatographic column, use the deionized water wash-out, coutroi velocity is 0.2～0.4mL/min, and 80%～95% the key component that peak area is accounted for each component total peak area is collected.At last, be-30～-45 ℃ at condenser temperature, vacuum is 50～80Pa, to moisture be less than or equal to till 8%, make refining active material after freeze-drying.
The above-mentioned self-dissolving operation of utilizing residue to prepare active material, preferred version is: after homogenate, the gained homogenate after the ultraviolet ray irradiation, adds citric acid and the disodium hydrogen phosphate buffer solution of 1～4 times of volume, pH4.0～7.0, carries out self-dissolving.In addition, under optimal way, when treating capacity was less, ultraviolet irradiation can be selected the 30W uviol lamp, induced apart from carrying out self-dissolving after 0.5m irradiation 5～40min; When treating capacity is larger, the sea cucumber intestine homogenate can be placed in stainless steel tank, slurries thickness is 1～2cm, and 0.2～0.5m places uviol lamp in the place up, and controlling slurries liquid level UV intensity is 60～120 μ W/cm
2, keep 5～40min to carry out self-dissolving and induce.
The advantage that the present invention gives prominence to is:
1. improve the utilization rate of Holothurian machining byproduct in process thing, its contained nutritional labeling and function factor are fully developed, also reduced simultaneously pollution on the environment.Sea cucumber intestine active material after processing is processed has certain oxidation resistance, can be used as the function base-material and is used for exploitation several functions type product, great exploitation potential for its.
2. the self-dissolving mentioned of the present invention is to utilize the contained endogenous enzymes of sea cucumber intestine self to carry out enzymolysis, adopts the self-dissolving technology to prepare the sea cucumber intestine active material, has kept to greatest extent the contained active component of sea cucumber intestine, obtains the more product of high-quality.Simultaneously, for the residue after the sea cucumber intestine self-dissolving, can adopt external source enzyme resolving to prepare active material, sea cucumber intestine is fully utilized.
The technological parameter of mentioning in selection process of the present invention, be intended to improve yield and the quality of active material product, under technological parameter condition of the present invention, in the sea cucumber intestine autolysate, the content of TCA solubility oligopeptides rises to before self-dissolving 2～3 times, and self-dissolving residue protein peptide yield after degenerative treatments, external source enzyme resolving reaches 10%～30%.
The specific embodiment
The present invention's a kind of sea cucumber intestine self-dissolving active material and self-dissolving residue active material thereof.Sea cucumber intestine self-dissolving active material is take sea cucumber intestine as raw material, through homogenate, self-dissolving, centrifugal, freeze concentration, gel column separates and freeze drying is made; Residue after the sea cucumber intestine self-dissolving can be through denatured pretreatment, external source enzyme resolving, centrifugal, freeze concentration, gel column separates and cryodesiccated mode is made.These two kinds of nutriment are the faint yellow solid powder, odorless, the intrinsic delicate flavour of sea cucumber intestine are arranged, and are soluble in water, and hygroscopicity is arranged, and have certain antioxidation in vitro ability, can be used as the function base-material for the preparation of several functions type food.
1. to prepare the concrete technology step as follows for sea cucumber intestine self-dissolving active material:
(1) raw material is processed
Take out sea cucumber intestine, cleaning from fresh sea cucumber, remove silt and impurity, directly homogenate is standby or freezing standby at-15 ℃;
(2) self-dissolving
Utilize the effect of the contained endogenous enzymes of sea cucumber intestine, make it carry out self-dissolving under different conditions:
The sea cucumber intestine homogenate adds the citric acid-disodium hydrogen phosphate buffer solution (pH4.0～7.0) of 1～4 times of volume, in 35 ℃～60 ℃ self-dissolving 0.5～4h or in 4 ℃～10 ℃ self-dissolving 4～12h after ultraviolet ray (30W, 0.5m) irradiation 5～40min.After self-dissolving finished, the centrifugal 10～30min of 2500～6000r/min got supernatant; Or the mode of employing ultrafiltration, collect the following component of 10kDa.Vacuum refrigeration is concentrated rear to 10%～20% of original volume, or directly freeze drying, is sea cucumber intestine self-dissolving active material crude product.
When treating capacity is larger, the sea cucumber intestine homogenate can be placed in stainless steel tank, slurries thickness is 1～2cm, and uviol lamp is placed at 0.2～0.5m place, top, and controlling slurries liquid level UV intensity is 60～120 μ W/cm
2, keep 5～40min to carry out self-dissolving and induce, add afterwards the citric acid-disodium hydrogen phosphate buffer solution (pH4.0～7.0) of 1～4 times of volume, carry out according to the method described above self-dissolving.
(3) separate
Sea cucumber intestine active material crude product is splined on Sephadex G-15 gel column, uses the deionized water wash-out, coutroi velocity is 0.3～0.5mL/min.Key component (peak area account for each component total peak area 70%～80%) is collected, reach simultaneously the purpose of desalination.
(4) drying
The active component of collecting is carried out freeze drying, and condenser temperature is-30～-45 ℃, and vacuum is 50～80Pa, to moisture be less than or equal to till 8%, namely obtain sea cucumber intestine self-dissolving active material after freeze-drying.
2. to prepare the concrete technology step as follows for sea cucumber intestine self-dissolving residue active material:
(1) pretreatment
After obtaining the sea cucumber intestine autolysate, get residue, add the deionized water mixing of 2～4 times of volumes, 90～100 ℃ of heating 5～15min carry out protein denaturation and process, and are cooling.
(2) external source enzyme resolving
At 50 ℃, under pH7.0, add the neutral proteinase (as bacillus subtilis 1398) of 1500～3000U/g albumen, enzymolysis 30～180min.After enzymolysis was complete, enzymolysis liquid was in 95～100 ℃ of heating 5～10min enzymes that go out, cooling after the centrifugal 10～30min of 2500～6000r/min, get supernatant, or adopt the mode of ultrafiltration, collect the following component of 10kDa, be sea cucumber intestine self-dissolving residue active material.This product can further adopt the method for freeze concentration to be concentrated into 10～20% of original volume; Can also adopt the mode of vacuum freeze drying to be dried to moisture less than or equal to 8%, thereby obtain the sea cucumber intestine self-dissolving residue active material crude product of different product form.
(3) separate
Sea cucumber intestine self-dissolving residue active material crude product is splined on sephadex Sephadex G-25 chromatographic column, use the deionized water wash-out, coutroi velocity is 0.2～0.4mL/min, key component (peak area account for each component total peak area 80%～95%) is collected, reach simultaneously the purpose of desalination.
(4) drying
The active component of collecting is carried out freeze drying, and condenser temperature is-30～-45 ℃, and vacuum is 50～80Pa, to moisture be less than or equal to till 8%, namely obtain sea cucumber intestine self-dissolving residue active material after freeze-drying.
Example one:
With the 100g sea cucumber intestine clean, shred, homogenate, ultraviolet ray (30W, 0.5m) irradiation 25min after homogenized adds sodium hydrogen phosphate-citric acid solution of the pH5.0 of 1.5 times of volumes, in 40 ℃ of self-dissolving 4h.After self-dissolving finished, the centrifugal 25min of 3000r/min got supernatant, and residue is standby.Supernatant is sea cucumber intestine self-dissolving active material crude product through 10% of vacuum refrigeration simmer down to original volume.Sea cucumber intestine separates through Sephadex G-15 gel column from dissolubility material crude product, and coutroi velocity is 0.4mL/min, collects key component, carries out freeze drying.Regulating condenser temperature is-30 ℃, and vacuum is 80Pa, less than 8%, namely obtains sea cucumber intestine self-dissolving active material to moisture after freeze-drying.
Sea cucumber intestine self-dissolving residue adds 4 times of volumes of deionized water mixings, 100 ℃ of heating 5min.After cooling, at 50 ℃, under pH7.0, add bacillus subtilis 1398 neutral proteinases of 1500U/g albumen, enzymolysis 180min.After enzymolysis was complete, enzymolysis liquid was in 95 ℃ of heating 10min enzymes that go out, and the centrifugal 10min of 6000r/min gets supernatant, and freeze concentration is sea cucumber intestine self-dissolving residue active material crude product to 10% of original volume.Concentrate is through sephadex Sephadex G-25 chromatographic column, use the deionized water wash-out, coutroi velocity is 0.25mL/min, collect key component, carry out freeze drying, condenser temperature is-30 ℃, and vacuum is 80Pa, to moisture be less than till 8%, namely obtain sea cucumber intestine self-dissolving residue active material after freeze-drying.Two kinds of products are the faint yellow solid powder, and odorless has the intrinsic delicate flavour of sea cucumber intestine, and is soluble in water, and hygroscopicity is arranged, and it has certain antioxidation in vitro ability, can be used as the function base-material for the preparation of several functions type food.
Example two:
With the 200g sea cucumber intestine clean, shred, homogenate, ultraviolet ray (30W, 0.5m) irradiation 40min after homogenized adds sodium hydrogen phosphate-citric acid solution of the pH6.0 of 3.5 times of volumes, in 50 ℃ of self-dissolving 3h.After self-dissolving finished, the centrifugal 20min of 4000r/min got supernatant, and residue is standby.Supernatant is sea cucumber intestine self-dissolving active material crude product through 20% of vacuum refrigeration simmer down to original volume.Sea cucumber intestine self-dissolving active material crude product separates through Sephadex G-15 gel column, and coutroi velocity is 0.5mL/min, collects key component, carries out freeze drying.Regulating condenser temperature is-45 ℃, and vacuum is 50Pa, less than 8%, namely obtains sea cucumber intestine self-dissolving active material to moisture content after freeze-drying.
Sea cucumber intestine self-dissolving residue adds 2 times of volumes of deionized water mixings, 90 ℃ of heating 15min.After cooling, at 50 ℃, under pH7.0, add the neutral proteinase of 2000U/g albumen, enzymolysis 120min.After enzymolysis was complete, enzymolysis liquid was in 100 ℃ of heating 5min enzymes that go out, and the 10kDa ultrafiltration is collected molecular weight lower than the component of 10kDa, and freeze concentration is sea cucumber intestine self-dissolving residue active material crude product to 15% of original volume.Concentrate is through sephadex Sephadex G-25 chromatographic column, use the deionized water wash-out, coutroi velocity is 0.3mL/min, collect key component, carry out freeze drying, condenser temperature is-45 ℃, and vacuum is 50Pa, to moisture be less than till 8%, namely obtain sea cucumber intestine self-dissolving residue active material after freeze-drying.Two kinds of products are the faint yellow solid powder, and odorless has the intrinsic delicate flavour of sea cucumber intestine, and is soluble in water, and hygroscopicity is arranged, and it has certain antioxidation in vitro ability, can be used as the function base-material for the preparation of several functions type food.
Example three:
With the 1kg sea cucumber intestine clean, shred, homogenate, be placed in stainless steel tank, the thickness of slurries is 1～2cm, 0.5m places uviol lamp in the place up, controlling slurries liquid level UV intensity is 60 μ W/cm
2, keep 15min to carry out self-dissolving and induce.Then, add sodium hydrogen phosphate-citric acid solution of the pH4.0 of 2 times of volumes, in 45 ℃ of self-dissolving 4h.After self-dissolving finished, the centrifugal 20min of 3500r/min got supernatant, and residue is standby.Supernatant is sea cucumber intestine self-dissolving active material crude product through 15% of vacuum refrigeration simmer down to original volume.Sea cucumber intestine self-dissolving active material crude product separates through Sephadex G-15 gel column, and coutroi velocity is 0.35mL/min, collects key component, carries out freeze drying.Regulating condenser temperature is-45 ℃, and vacuum is 65Pa, less than 8%, namely obtains sea cucumber intestine self-dissolving active material to moisture after freeze-drying.
Sea cucumber intestine self-dissolving residue adds 3 times of volumes of deionized water mixings, 95 ℃ of heating 10min.After cooling, at 50 ℃, under pH7.0, add bacillus subtilis 1398 neutral proteinases of 3000U/g albumen, enzymolysis 60min.After enzymolysis was complete, enzymolysis liquid was in 100 ℃ of heating 5min enzymes that go out, and the 10kDa ultrafiltration is collected molecular weight lower than the component of 10kDa, and freeze concentration is sea cucumber intestine self-dissolving residue active material crude product to 15% of original volume.Concentrate is through sephadex Sephadex G-25 chromatographic column, use the deionized water wash-out, coutroi velocity is 0.25mL/min, collect key component, carry out freeze drying, condenser temperature is-45 ℃, and vacuum is 65Pa, to moisture be less than till 8%, namely obtain sea cucumber intestine self-dissolving residue active material after freeze-drying.Two kinds of products are the faint yellow solid powder, and odorless has the intrinsic delicate flavour of sea cucumber intestine, and is soluble in water, and hygroscopicity is arranged, and it has certain antioxidation in vitro ability, can be used as the function base-material for the preparation of several functions type food.
Example four:
With the 500g sea cucumber intestine clean, shred, homogenate, ultraviolet ray (30W, 0.5m) irradiation 10min after homogenized adds sodium hydrogen phosphate-citric acid solution of the pH4.0 of 4 times of volumes, in 60 ℃ of self-dissolving 0.5h.After self-dissolving finished, the centrifugal 15min of 4500r/min got supernatant, and residue is standby.Supernatant is sea cucumber intestine self-dissolving active material crude product through 20% of vacuum refrigeration simmer down to original volume.Sea cucumber intestine self-dissolving active material crude product separates through Sephadex G-15 gel column, and coutroi velocity is 0.4mL/min, collects key component, carries out freeze drying.Regulating condenser temperature is-35 ℃, and vacuum is 75Pa, less than 6%, namely obtains sea cucumber intestine self-dissolving active material to moisture after freeze-drying.
Sea cucumber intestine self-dissolving residue adds 2 times of volumes of deionized water mixings, 100 ℃ of heating 5min.After cooling, at 50 ℃, under pH7.0, add the neutral proteinase of 2000U/g albumen, enzymolysis 150min.After enzymolysis was complete, enzymolysis liquid was in 95 ℃ of heating 10min enzymes that go out, and the centrifugal 15min of 4500r/min gets supernatant, through 20% of vacuum refrigeration simmer down to original volume, is sea cucumber intestine self-dissolving residue active material crude product.Concentrate is through sephadex Sephadex G-25 chromatographic column, use the deionized water wash-out, coutroi velocity is 0.3mL/min, collect key component, carry out freeze drying, condenser temperature is-35 ℃, and vacuum is 75Pa, to moisture be less than till 8%, namely obtain sea cucumber intestine self-dissolving residue active material after freeze-drying.Two kinds of products are the faint yellow solid powder, and odorless has the intrinsic delicate flavour of sea cucumber intestine, and is soluble in water, and hygroscopicity is arranged, and it has certain antioxidation in vitro ability, can be used as the function base-material for the preparation of several functions type food.
Example five:
With the 300g sea cucumber intestine clean, shred, homogenate, ultraviolet ray (30W, 0.5m) irradiation 30min after homogenized adds sodium hydrogen phosphate-citric acid solution of the pH5.0 of 1 times of volume, in 35 ℃ of self-dissolving 6h.After self-dissolving finished, the centrifugal 15min of 5000r/min got supernatant, and residue is standby.Supernatant is sea cucumber intestine self-dissolving active material crude product through 20% of vacuum refrigeration simmer down to original volume.Sea cucumber intestine self-dissolving active material crude product separates through Sephadex G-15 gel column, and coutroi velocity is 0.5mL/min, collects key component, carries out freeze drying.Regulating condenser temperature is-40 ℃, and vacuum is 70Pa, less than 6%, namely obtains sea cucumber intestine self-dissolving active material to moisture after freeze-drying.
Sea cucumber intestine self-dissolving residue adds 4 times of volumes of deionized water mixings, 90 ℃ of heating 10min.After cooling, at 50 ℃, under pH7.0, add the neutral proteinase of 1500U/g albumen, enzymolysis 180min.After enzymolysis was complete, enzymolysis liquid was in 100 ℃ of heating 5min enzymes that go out, and the centrifugal 15min of 5000r/min gets supernatant, and supernatant is sea cucumber intestine self-dissolving residue active material crude product through 15% of vacuum refrigeration simmer down to original volume.Concentrate is through sephadex Sephadex G-25 chromatographic column, use the deionized water wash-out, coutroi velocity is 0.4mL/min, collect key component, carry out freeze drying, condenser temperature is-40 ℃, and vacuum is 70Pa, to moisture be less than till 8%, namely obtain sea cucumber intestine self-dissolving residue active material after freeze-drying.Two kinds of products are the faint yellow solid powder, and odorless has the intrinsic delicate flavour of sea cucumber intestine, and is soluble in water, and hygroscopicity is arranged, and it has certain antioxidation in vitro ability, can be used as the function base-material for the preparation of several functions type food.
Example six:
With the 1.5kg sea cucumber intestine clean, shred, homogenate, be placed in stainless steel tank, the thickness of slurries is 2cm, 0.3m places uviol lamp in the place up, controls slurries liquid level UV intensity at 100 μ W/cm
2, keep 20min to carry out self-dissolving and induce.Then, add sodium hydrogen phosphate-citric acid solution of the pH7.0 of 3 times of volumes, in 50 ℃ of self-dissolving 2h.After self-dissolving finished, the centrifugal 30min of 2500r/min got supernatant, and residue is standby.Supernatant is sea cucumber intestine self-dissolving active material crude product through 20% of vacuum refrigeration simmer down to original volume.Sea cucumber intestine self-dissolving active material crude product separates through Sephadex G-15 gel column, and coutroi velocity is 0.3mL/min, collects key component, carries out freeze drying.Regulating condenser temperature is-45 ℃, and vacuum is 70Pa, less than 6%, namely obtains sea cucumber intestine self-dissolving active material to moisture after freeze-drying.
Sea cucumber intestine self-dissolving residue adds 2 times of volumes of deionized water mixings, 100 ℃ of heating 5min.After cooling, at 50 ℃, under pH7.0, add the neutral proteinase of 2500U/g albumen, enzymolysis 120min.After enzymolysis was complete, enzymolysis liquid was in 95 ℃ of heating 10min enzymes that go out, and the centrifugal 30min of 2500r/min gets supernatant, and supernatant to 10% of original volume, is sea cucumber intestine self-dissolving residue active material crude product through freeze concentration.Concentrate is through sephadex Sephadex G-25 chromatographic column, use the deionized water wash-out, coutroi velocity is 0.3mL/min, collect key component, carry out freeze drying, condenser temperature is-45 ℃, and vacuum is 70Pa, to moisture be less than till 8%, namely obtain sea cucumber intestine self-dissolving residue active material after freeze-drying.Two kinds of products are the faint yellow solid powder, and odorless has the intrinsic delicate flavour of sea cucumber intestine, and is soluble in water, and hygroscopicity is arranged, and it has certain antioxidation in vitro ability, can be used as the function base-material for the preparation of several functions type food.
Example seven:
With the 800g sea cucumber intestine clean, shred, homogenate, ultraviolet ray (30W, 0.5m) irradiation 5min after homogenized adds sodium hydrogen phosphate-citric acid solution of the pH6.0 of 4 times of volumes, in 60 ℃ of self-dissolving 0.5h.After self-dissolving finished, the centrifugal 10min of 5500r/min got supernatant, and residue is standby.Supernatant is sea cucumber intestine active material crude product after vacuum refrigeration is done.The dissolving of sea cucumber intestine active material crude product water separates through Sephadex G-15 gel column, and coutroi velocity is 0.45mL/min, collects key component, carries out freeze drying.Regulating condenser temperature is-35 ℃, and vacuum is 60Pa, less than 6%, namely obtains sea cucumber intestine self-dissolving active material to moisture after freeze-drying.
Sea cucumber intestine self-dissolving residue adds 3 times of volumes of deionized water mixings, 90 ℃ of heating 15min.After cooling, at 50 ℃, under pH7.0, add the neutral proteinase of 3000U/g albumen, enzymolysis 120min.After enzymolysis was complete, enzymolysis liquid was in 100 ℃ of heating 5min enzymes that go out, and the 10kDa ultrafiltration is collected molecular weight lower than the component of 10kDa, and freeze concentration is sea cucumber intestine self-dissolving residue active material crude product to 15% of original volume.Concentrate is through sephadex Sephadex G-25 chromatographic column, use the deionized water wash-out, coutroi velocity is 0.25mL/min, collect key component, carry out freeze drying, condenser temperature is-35 ℃, and vacuum is 60Pa, to moisture be less than till 8%, namely obtain sea cucumber intestine self-dissolving residue active material after freeze-drying.Two kinds of products are the faint yellow solid powder, and odorless has the intrinsic delicate flavour of sea cucumber intestine, and is soluble in water, and hygroscopicity is arranged, and it has certain antioxidation in vitro ability, can be used as the function base-material for the preparation of several functions type food.
Example eight:
With the 2kg sea cucumber intestine clean, shred, homogenate, be placed in stainless steel tank, the thickness of slurries is 1cm, 0.5m places uviol lamp in the place up, making slurries liquid level UV intensity is 100 μ W/cm
2, keep 20min to carry out self-dissolving and induce.Then, add sodium hydrogen phosphate-citric acid solution of the pH4.0 of 4 times of volumes, in 55 ℃ of self-dissolving 1.5h.After self-dissolving finished, the centrifugal 10min of 6000r/min got supernatant, and residue is standby.Supernatant is sea cucumber intestine self-dissolving active material crude product after vacuum refrigeration is concentrated.Then separate through Sephadex G-15 gel column, coutroi velocity is 0.5mL/min, collects key component, carries out freeze drying.Regulating condenser temperature is-35 ℃, and vacuum is 65Pa, less than 6%, namely obtains sea cucumber intestine self-dissolving active material to moisture after freeze-drying.
Sea cucumber intestine self-dissolving residue adds 4 times of volumes of deionized water mixings, 90 ℃ of heating 15min.After cooling, at 50 ℃, under pH7.0, add bacillus subtilis 1398 neutral proteinases of 2000U/g albumen, enzymolysis 180min.After enzymolysis was complete, enzymolysis liquid was in 95 ℃ of heating 5min enzymes that go out, and the centrifugal 10min of 6000r/min gets supernatant, and supernatant is sea cucumber intestine self-dissolving residue active material crude product through 15% of vacuum refrigeration simmer down to original volume.Concentrate is through sephadex Sephadex G-25 chromatographic column, use the deionized water wash-out, coutroi velocity is 0.4mL/min, collect key component, carry out freeze drying, condenser temperature is-35 ℃, and vacuum is 65Pa, to moisture be less than till 8%, namely obtain sea cucumber intestine self-dissolving residue active material after freeze-drying.Two kinds of products are the faint yellow solid powder, and odorless has the intrinsic delicate flavour of sea cucumber intestine, and is soluble in water, and hygroscopicity is arranged, and it has certain antioxidation in vitro ability, can be used as the function base-material for the preparation of several functions type food.
The above; only be the better specific embodiment of the present invention; but protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to replacement or changed according to technical scheme of the present invention and inventive concept thereof, within all should being encompassed in protection scope of the present invention.
Claims (10)
1. method of utilizing the sea cucumber intestine self-dissolving to prepare active material, it is characterized in that, take sea cucumber intestine as raw material, through homogenate, self-dissolving, get supernatant, freeze concentration less than or equal to 8%, makes sea cucumber intestine active material crude product to 10%～20% or vacuum freeze drying of original volume to moisture; Make refining active material through separation, freeze drying again.
2. utilize according to claim 1 the sea cucumber intestine self-dissolving to prepare the method for active material, it is characterized in that, the operation of described self-dissolving is:
After homogenate, the gained homogenate after the ultraviolet ray irradiation, adds citric acid and the disodium hydrogen phosphate buffer solution of 1～4 times of volume, pH 4.0～7.0, at 35～60 ℃ of lower self-dissolving 0.5～4h, or in the time of 4～10 ℃, self-dissolving 4～12h.
3. utilize according to claim 2 the sea cucumber intestine self-dissolving to prepare the method for active material, it is characterized in that, select 30 watts of uviol lamps in described self-dissolving operation, apart from 0.5m irradiation 5～40min, carry out self-dissolving and induce;
Perhaps, homogenate is placed in stainless steel tank, 0.2～0.5m places uviol lamp in the place up, and controlling slurries liquid level UV intensity is 60～120 μ W/cm
2, keep 5～40min to carry out self-dissolving and induce.
4. utilize according to claim 3 the sea cucumber intestine self-dissolving to prepare the method for active material, it is characterized in that, described operation of getting supernatant is: centrifugal 10～30 minutes of 2500～6000r/min, get supernatant; Perhaps described operation of getting supernatant adopts the mode of ultrafiltration, collects the following component of 10kDa.
5. according to claim 1-4 arbitrary described methods of utilizing the sea cucumber intestine self-dissolving to prepare active material, is characterized in that, the operation of described separation is:
Described sea cucumber intestine active material crude product is splined on sephadex Sephadex G-15 chromatographic column, uses the deionized water wash-out, coutroi velocity is 0.3～0.5mL/min, and the active component that peak area is accounted for each component total peak area 70%～80% is collected.
6. utilize according to claim 5 the sea cucumber intestine self-dissolving to prepare the method for active material, it is characterized in that, described cryodesiccated operation is: in condenser temperature-30～-45 ℃, vacuum is to carry out drying under 50～80Pa condition.
7. a method of utilizing the sea cucumber intestine self-dissolving to prepare active material, is characterized in that, take sea cucumber intestine as raw material, through homogenate, self-dissolving;
Then get residue, add the deionized water mixing of 2～4 times of volumes, 90 ℃～100 ℃ heating 5～15min; Under 50 ℃, pH7.0, add the neutral proteinase of 1500～3000U/g albumen, enzymolysis 30～180min after cooling;
Enzymolysis liquid is in 95～100 ℃ of heating 5～10min enzymes that go out, cooling after under 2500～6000r/min centrifugal 10～30min;
Then get supernatant or adopt the mode of ultrafiltration to collect the following component of 10kDa, make sea cucumber intestine self-dissolving residue active material.
8. utilize according to claim 7 the sea cucumber intestine self-dissolving to prepare the method for active material, it is characterized in that, the operation of described self-dissolving is:
After homogenate, the gained homogenate after the ultraviolet ray irradiation, adds citric acid and the disodium hydrogen phosphate buffer solution of 1～4 times of volume, pH 4.0～7.0, at 35～60 ℃ of lower self-dissolving 0.5～4h, or in the time of 4 ℃～10 ℃, self-dissolving 4～12h.
Wherein, the operation of ultraviolet ray irradiation is: select 30 watts of uviol lamps in described self-dissolving operation, carry out self-dissolving apart from 0.5m irradiation 5～40min and induce; Perhaps, homogenate is placed in stainless steel tank, slurries thickness 1～2cm, 0.2～0.5m places uviol lamp in the place up, and controlling slurries liquid level UV intensity is 60～120 μ W/cm
2, keep 5～40min to carry out self-dissolving and induce.
9. the according to claim 7 or 8 described methods of utilizing the sea cucumber intestine self-dissolving to prepare active material, it is characterized in that, adopt the method for freeze concentration to be concentrated into 10%～20% of original volume the sea cucumber intestine self-dissolving residue active material that makes, perhaps adopt the mode of vacuum freeze drying to be dried to moisture less than or equal to 8%, obtain sea cucumber intestine self-dissolving residue active material crude product.
10. utilize according to claim 9 the sea cucumber intestine self-dissolving to prepare the method for active material, it is characterized in that, described sea cucumber intestine self-dissolving residue active material crude product is splined on sephadex Sephadex G-25 chromatographic column, use the deionized water wash-out, coutroi velocity is 0.2～0.4mL/min, and 80%～95% the key component that peak area is accounted for each component total peak area is collected;
At last, be-30～-45 ℃ at condenser temperature, vacuum is to carry out drying under 50～80Pa condition, less than or equal to till 8%, makes refining active material to moisture after freeze-drying.
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| CN2011104357118A CN103141872A (en) | 2011-12-22 | 2011-12-22 | Method for preparing active substance by sea cucumber intestine autolysis |
| JP2013549709A JP2014504160A (en) | 2011-12-22 | 2012-02-20 | Production method of active material by self-digestion of sea cucumber intestine |
| PCT/CN2012/071381 WO2013091301A1 (en) | 2011-12-22 | 2012-02-20 | Method of use of sea cucumber intestine autolysis for preparing active substance |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104544454A (en) * | 2014-12-19 | 2015-04-29 | 渤海大学 | Sea cucumber peptide nutrition powder and preparation method thereof |
| CN105105118A (en) * | 2015-09-01 | 2015-12-02 | 大连工业大学 | Method for preparing sea cucumber intestine lipid rich in EPA |
| CN107712692A (en) * | 2017-11-23 | 2018-02-23 | 黑龙江美丽石岛科技开发有限公司 | A kind of method that nutriment is extracted from unsalted dried sea cucumbers |
| CN107927764A (en) * | 2017-11-20 | 2018-04-20 | 黑龙江美丽石岛科技开发有限公司 | A kind of health food containing sea cucumber extract and earthworm albumen powder and preparation method thereof |
| CN110279108A (en) * | 2019-06-27 | 2019-09-27 | 烟台东宇海珍品有限公司 | A kind of preparation method for the sea cucumber product that human body easily absorbs |
| CN110353197A (en) * | 2018-03-26 | 2019-10-22 | 大连凯林生物科技有限公司 | Sea cucumber rice noodle roll and sea cucumber enteral nutrient solution and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107801974A (en) * | 2017-12-21 | 2018-03-16 | 大连深蓝肽科技研发有限公司 | A kind of preparation method of sea cucumber peptide salt |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20070105714A (en) * | 2006-04-27 | 2007-10-31 | 이주석 | Method for preparation of capsule of seacucumber peptide |
| CN101173261B (en) * | 2007-10-29 | 2010-08-25 | 大连工业大学 | Separation purification technique for lysozyme in holothurian intestines |
| JP2009189333A (en) * | 2008-02-18 | 2009-08-27 | Kumakan Shoten:Kk | Powder made of sea cucumber entrails and method for producing the same |
| CN101361578B (en) * | 2008-09-25 | 2011-05-11 | 大连工业大学 | Beche-de-mer ovum nurture and preparation method thereof |
-
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104544454A (en) * | 2014-12-19 | 2015-04-29 | 渤海大学 | Sea cucumber peptide nutrition powder and preparation method thereof |
| CN105105118A (en) * | 2015-09-01 | 2015-12-02 | 大连工业大学 | Method for preparing sea cucumber intestine lipid rich in EPA |
| CN107927764A (en) * | 2017-11-20 | 2018-04-20 | 黑龙江美丽石岛科技开发有限公司 | A kind of health food containing sea cucumber extract and earthworm albumen powder and preparation method thereof |
| CN107712692A (en) * | 2017-11-23 | 2018-02-23 | 黑龙江美丽石岛科技开发有限公司 | A kind of method that nutriment is extracted from unsalted dried sea cucumbers |
| CN110353197A (en) * | 2018-03-26 | 2019-10-22 | 大连凯林生物科技有限公司 | Sea cucumber rice noodle roll and sea cucumber enteral nutrient solution and preparation method thereof |
| CN110279108A (en) * | 2019-06-27 | 2019-09-27 | 烟台东宇海珍品有限公司 | A kind of preparation method for the sea cucumber product that human body easily absorbs |
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| JP2014504160A (en) | 2014-02-20 |
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