CN110353197A - Sea cucumber rice noodle roll and sea cucumber enteral nutrient solution and preparation method thereof - Google Patents
Sea cucumber rice noodle roll and sea cucumber enteral nutrient solution and preparation method thereof Download PDFInfo
- Publication number
- CN110353197A CN110353197A CN201810254831.XA CN201810254831A CN110353197A CN 110353197 A CN110353197 A CN 110353197A CN 201810254831 A CN201810254831 A CN 201810254831A CN 110353197 A CN110353197 A CN 110353197A
- Authority
- CN
- China
- Prior art keywords
- sea cucumber
- self
- cucumber intestine
- dissolving
- intestine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000251511 Holothuroidea Species 0.000 title claims abstract description 239
- 238000002360 preparation method Methods 0.000 title claims abstract description 45
- 235000015097 nutrients Nutrition 0.000 title claims abstract description 40
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 31
- 235000012149 noodles Nutrition 0.000 title claims abstract description 31
- 235000009566 rice Nutrition 0.000 title claims abstract description 31
- 240000007594 Oryza sativa Species 0.000 title abstract 2
- 210000000936 intestine Anatomy 0.000 claims abstract description 167
- 238000002525 ultrasonication Methods 0.000 claims abstract description 68
- 108090000790 Enzymes Proteins 0.000 claims abstract description 54
- 102000004190 Enzymes Human genes 0.000 claims abstract description 54
- 238000000605 extraction Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 83
- 239000006228 supernatant Substances 0.000 claims description 68
- 229940088598 enzyme Drugs 0.000 claims description 52
- 239000007788 liquid Substances 0.000 claims description 43
- 238000000108 ultra-filtration Methods 0.000 claims description 36
- 238000000034 method Methods 0.000 claims description 34
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 30
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 30
- 239000000284 extract Substances 0.000 claims description 30
- 238000003756 stirring Methods 0.000 claims description 30
- 241000209094 Oryza Species 0.000 claims description 29
- 238000002791 soaking Methods 0.000 claims description 29
- 239000012528 membrane Substances 0.000 claims description 24
- 239000003814 drug Substances 0.000 claims description 23
- 230000008569 process Effects 0.000 claims description 21
- 238000005119 centrifugation Methods 0.000 claims description 18
- 230000009849 deactivation Effects 0.000 claims description 18
- 239000012530 fluid Substances 0.000 claims description 18
- 238000001728 nano-filtration Methods 0.000 claims description 18
- 239000008213 purified water Substances 0.000 claims description 18
- 238000012545 processing Methods 0.000 claims description 16
- 238000000926 separation method Methods 0.000 claims description 15
- 239000000470 constituent Substances 0.000 claims description 14
- 238000000502 dialysis Methods 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 12
- 238000004108 freeze drying Methods 0.000 claims description 12
- 239000000047 product Substances 0.000 claims description 12
- 241000208340 Araliaceae Species 0.000 claims description 10
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 10
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 10
- 108090000526 Papain Proteins 0.000 claims description 10
- 239000004365 Protease Substances 0.000 claims description 10
- 238000007710 freezing Methods 0.000 claims description 10
- 230000008014 freezing Effects 0.000 claims description 10
- 235000008434 ginseng Nutrition 0.000 claims description 10
- 229940055729 papain Drugs 0.000 claims description 10
- 235000019834 papain Nutrition 0.000 claims description 10
- 238000010257 thawing Methods 0.000 claims description 10
- 235000005979 Citrus limon Nutrition 0.000 claims description 9
- 244000131522 Citrus pyriformis Species 0.000 claims description 9
- 239000002253 acid Substances 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 239000012141 concentrate Substances 0.000 claims description 9
- 239000002002 slurry Substances 0.000 claims description 9
- 244000185386 Thladiantha grosvenorii Species 0.000 claims description 8
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 8
- 108090000145 Bacillolysin Proteins 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 6
- 239000012466 permeate Substances 0.000 claims description 6
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000009738 saturating Methods 0.000 claims description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 43
- 239000004615 ingredient Substances 0.000 abstract description 38
- 230000000717 retained effect Effects 0.000 abstract description 3
- 239000000843 powder Substances 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 30
- 239000002699 waste material Substances 0.000 description 9
- 230000007613 environmental effect Effects 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 230000035764 nutrition Effects 0.000 description 5
- 230000000050 nutritive effect Effects 0.000 description 5
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 description 4
- 239000002994 raw material Substances 0.000 description 3
- 238000012958 reprocessing Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 235000014102 seafood Nutrition 0.000 description 2
- 229910052720 vanadium Inorganic materials 0.000 description 2
- LEONUFNNVUYDNQ-UHFFFAOYSA-N vanadium atom Chemical compound [V] LEONUFNNVUYDNQ-UHFFFAOYSA-N 0.000 description 2
- 241000258955 Echinodermata Species 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 108010024636 Glutathione Proteins 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 208000000718 duodenal ulcer Diseases 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 201000005917 gastric ulcer Diseases 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 230000036642 wellbeing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/70—Comminuted, e.g. emulsified, fish products; Processed products therefrom such as pastes, reformed or compressed products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Marine Sciences & Fisheries (AREA)
- Mycology (AREA)
- Botany (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention provides the sea cucumber rice noodle roll and sea cucumber enteral nutrient solution preparation method of a kind of sea cucumber intestine powder, preparation method thereof and its preparation and its sea cucumber enteral nutrient solutions of preparation, these preparation methods can make full use of endogenous enzymes and carry out self-dissolving, and it is reprocessed for a large amount of nutritional ingredients present in self-dissolving residue, nutritional ingredient in sea cucumber intestine is further extracted by means of ultrasonication, so as to avoid the loss of sea cucumber intestine nutritional ingredient, and it is further handled by means of external source enzyme resolving so that nutritional ingredient has obtained further extraction, avoid the loss of nutritional ingredient, improve the utilization rate of sea cucumber intestine, so that sea cucumber intestine nutritional ingredient as much as possible is retained.
Description
Technical field
The present invention relates to a kind of sea cucumber rice noodle roll and sea cucumber enteral nutrient solutions and preparation method thereof, belong to seafood processing neck
Domain.
Background technique
Sea cucumber category invertebrate Echinodermata, due to its higher nutritive value and pharmacy value, and is listed in " eight
One of it is precious ", belong to precious seafood delights.Sea cucumber is considered as nourishing food in traditional habit.Through scientific validation, more than 50 kinds are contained in sea cucumber body
The nutritional ingredient beneficial to human physiological activity, wherein protein content is higher, and microelement type is abundant, can continue human body
Aging is tonified Qi of the kidney, essence-replenishing and marrow-strengthening, eliminates fatigue, with more anticoagulation, antitumor, antibacterial, antiviral and improve immunity
Effect.
In recent years, the enterprise of processing sea cucumber is also more and more, but sea cucumber intestine carries a large amount of silts, enteral contain it is a large amount of different
Object cannot mechanically break intestines because sea cucumber intestine is extremely soft, and the prior art cannot be removed effectively silt and foreign body in intestine, sternly
Hinder the processing and utilization of sea cucumber intestine again, but nutritional ingredient contained in sea cucumber intestine discarded during Holothurian machining, no
It is second to body wall, dry intestines rate containing vanadium is 12 parts of million parts, about 3 times higher than rate containing vanadium in its body.Modern medicine is it has been shown that sea
It is sweet, warm to join intestines nature and flavor, enters stomach meridian, there is warming middle-Jiao and tonifying deficiency analgesic efficacy, gastric and duodenal ulcer can be treated, maintains normal sugar
Metabolism stimulates the hematopoiesis function of body, promotes the hematopoietic potential of bone.Thus the utilization of sea cucumber intestine resource increasingly causes
Pay attention to, in order to improve sea cucumber comprehensive processing and utilization rate, meet market needs, increase economic well-being of workers and staff, urgent need is found a kind of efficiently honest and clean
The processing method of valence handles sea cucumber intestine, to which sea cucumber intestine can be efficiently used, the rich and varied sea that is made into
Join intestines product.
There are great number of issues in the processing of existing sea cucumber intestine, first, in preprocessing process often using sodium bicarbonate, ethyl alcohol,
Water-insoluble glucan suspension, glutathione aqueous solution etc. pre-process sea cucumber intestine, but these solution and/or substance for
The nutritional ingredient of sea cucumber intestine affects and is difficult to be kept completely separate；Second, it solely uses during the extraction process
Endogenous enzymes self-dissolving, ultrasonication, external source enzyme resolving cause the nutrition in a large amount of residue that cannot effectively obtain, serious shadow
The utilization for having rung sea cucumber intestine nutritional ingredient causes a large amount of waste, but also the nutritional ingredient of obtained sea cucumber intestine product is remote
Far fewer than sea cucumber intestine；Third leads to sea cucumber intestine when combining manufacture and use between sea cucumber intestine product and other Chinese medicines, nutriment
Nutritional ingredient and other between be limited to various environmental conditions and ingredient interferes with each other, or even can have a negative impact to body
Ingredient.
Summary of the invention
The first purpose of the invention is to provide a kind of to be combined endogenous enzymes self-dissolving and ultrasonication to obtain sea
Residue after self-dissolving can be carried out ultrasonic wave and located again by the preparation method of ginseng rice noodle roll and its prepared sea cucumber rice noodle roll
Reason, to effectively avoid waste caused by self-dissolving, extracts the nutritional ingredient as much as possible of sea cucumber intestine, simultaneously
Entire preparation method does not use any natural constituent, so that prepared sea cucumber rice noodle roll is added without any non-natural, effectively
Guarantee the safety and natural sex that it is used.
The first purpose of this invention is achieved through the following technical solutions: a kind of preparation method of sea cucumber rice noodle roll, should
Preparation method carries out as follows:
Step 1: sea cucumber intestine pre-processes: 1) collecting fresh sea cucumber intestine；2) pure water pre-soaking sea cucumber intestine is used, when pre-soaking
Between 1.5 hours and every 0.5 hour stir 1 time, then by the sea cucumber intestine through pre-soaking repeatedly with pure water impregnate five times, every time
It impregnates 0.5 hour；3) it freezes, the sea cucumber intestine taking-up after pure water is impregnated repeatedly is refrigerated to complete knot at a temperature of being placed in -4 DEG C
Block；4) it thaws: the sea cucumber intestine after freezing being placed in pure water after thawing and continue to impregnate 0.5 hour in pure water, take out drip
Clean transparent sea cucumber intestine is obtained after water；
Step 2: the homogeneous of sea cucumber intestine:
It will be crushed and be slurried using wet process high speed through the pretreated sea cucumber intestine of step 1, and suitable pure water is added, carried out equal
Matter obtains sea cucumber intestine homogenizing fluid；
Step 3: self-dissolving:
The sea cucumber intestine homogenizing fluid that will be obtained through step 2 makes it under the following conditions using the effect of endogenous enzymes contained by sea cucumber intestine
Self-dissolving: placing it in below ultraviolet radiator at 0.4 meter, and control slurries liquid level UV intensity is 100W/ cm2, it irradiates 0.5 hour,
Appropriate natural lemon acid is added, pH value is adjusted to pH5.0-6.5, keeps 4 DEG C of self-dissolvings 10 hours；Product after self-dissolving is placed in horizontal
Screw settling centrifuge, and centrifugation 0.5 hour is carried out with 3500r/min, obtain self-dissolving supernatant and self-dissolving residue；
Step 4: ultrasonication:
1) self-dissolving residue obtained in step 3 is inputted in vacuum ultrasonic reaction kettle；2) it is evacuated to 100Pa, controls kettle
0 DEG C of interior temperature；3) power for adjusting ultrasonic transmitter is 500W, sets ultrasonic wave tranmitting frequency as 25KHz, the pulse period is frequently
15 time/second of rate, 1700 meter per second of acoustic beam, liquid surface sound pressure level are 20 °, start ultrasonic wave and control ultrasonic intensity, make self-dissolving
Residue generates vortex, ultrasonication 2.5 hours, obtains sea cucumber intestine active component extract；4) sea cucumber active constituent is mentioned
Object is taken, in addition tube centrifuge in batches, starting centrifuge starts, and sets revolution as 15000r/min, centrifugation time 1 is small
When, temperature is set as 0 DEG C, and separation obtains ultrasonication supernatant and ultrasonication residue；
Step 5: hybrid filtering
The self-dissolving supernatant that step 3 obtains is mixed with the ultrasonication supernatant that step 4 obtains, is stirred evenly,
The ultrafiltration membrane ultrafiltration for being 6000-7000Da with molecular cut off obtains ultrafiltration permeate, is then diluted with appropriate amount of purified water, then with cut
Staying molecular weight is that the nanofiltration membrane of 400-600Da carries out dialysis concentration, obtains nanofiltration concentrate；
Step 6: freeze-drying:
Step 5 nanofiltration concentrate obtained is removed into moisture by the way of freeze-drying and obtains powdered sea cucumber rice noodle roll.
Endogenous enzymes self-dissolving, ultrasonication and external source enzyme resolving are carried out a second object of the present invention is to provide a kind of
In conjunction with come obtain sea cucumber rice noodle roll preparation method and its prepared sea cucumber rice noodle roll, the residue after self-dissolving can be carried out
Ultrasonic wave reprocessing then also carries out ultrasonication residue to effectively avoid waste caused by endogenous enzymes self-dissolving
External source enzyme resolving processing, so that waste caused by ultrasonication is further effectively prevented, so that sea cucumber intestine is as far as possible
More nutritional ingredients are extracted, while entire preparation method does not use any natural constituent, so that prepared sea cucumber
The safety and natural sex that it is used is effectively ensured without any artificial addition in rice noodle roll.
Second object of the present invention is achieved through the following technical solutions: a kind of preparation method of sea cucumber rice noodle roll, should
Preparation method carries out as follows:
Step 1: sea cucumber intestine pre-processes: 1) collecting fresh sea cucumber intestine；2) pure water pre-soaking sea cucumber intestine is used, when pre-soaking
Between 1.5 hours and every 0.5 hour stir 1 time, then by the sea cucumber intestine through pre-soaking repeatedly with pure water impregnate five times, every time
It impregnates 0.5 hour；3) it freezes, the sea cucumber intestine taking-up after pure water is impregnated repeatedly is refrigerated to complete knot at a temperature of being placed in -4 DEG C
Block；4) it thaws: the sea cucumber intestine after freezing being placed in pure water after thawing and continue to impregnate 0.5 hour in pure water, take out drip
Clean transparent sea cucumber intestine is obtained after water；
Step 2: the homogeneous of sea cucumber intestine:
It will be crushed and be slurried using wet process high speed through the pretreated sea cucumber intestine of step 1, and suitable pure water is added, carried out equal
Matter obtains sea cucumber intestine homogenizing fluid；
Step 3: self-dissolving:
The sea cucumber intestine homogenizing fluid that will be obtained through step 2 makes it under the following conditions using the effect of endogenous enzymes contained by sea cucumber intestine
Self-dissolving: placing it in below ultraviolet radiator at 0.4 meter, and control slurries liquid level UV intensity is 100W/ cm2, it irradiates 0.5 hour,
Appropriate natural lemon acid is added, pH value is adjusted to pH5.0-6.5, keeps 4 DEG C of self-dissolvings 10 hours；Product after self-dissolving is placed in horizontal
Screw settling centrifuge, and centrifugation 0.5 hour is carried out with 3500r/min, obtain self-dissolving supernatant and self-dissolving residue；
Step 4: ultrasonication:
1) self-dissolving residue obtained in step 3 is inputted in vacuum ultrasonic reaction kettle；2) it is evacuated to 100Pa, controls kettle
0 DEG C of interior temperature；3) power for adjusting ultrasonic transmitter is 500W, sets ultrasonic wave tranmitting frequency as 25KHz, the pulse period is frequently
15 time/second of rate, 1700 meter per second of acoustic beam, liquid surface sound pressure level are 20 °, start ultrasonic wave and control ultrasonic intensity, make self-dissolving
Residue generates vortex, ultrasonication 2.5 hours, obtains sea cucumber intestine active component extract；4) sea cucumber active constituent is mentioned
Object is taken, in addition tube centrifuge in batches, starting centrifuge starts, and sets revolution as 15000r/min, centrifugation time 1 is small
When, temperature is set as 0 DEG C, and separation obtains ultrasonication supernatant and ultrasonication residue；
Step 5: enzymolysis processing；
1) appropriate amount of purified water will be added through the separating obtained ultrasonication residue of step 4, stirs evenly, is heated to 100 DEG C
Kept for 0.5 hour, be sufficiently swollen, 2) it is then cooled to 45-50 DEG C, it adjusts pH value to 6.5-6.7, is added in right amount by bacillus subtilis
Neutral proteinase obtained by the fermented extraction of bacterium, stirs evenly, enzymatic hydrolysis 2.5 hours, 3) 55-60 DEG C is then heated to, adjust pH value
To 6.8-7.0, papain is added, stirs evenly, digest 3.5 hours, be then cooled to 4 DEG C, obtains enzymolysis liquid；4) by enzyme
It solves liquid and utilizes disk plate centrifuge, and separation 30 minutes is carried out with 6000r/min, obtain enzymatic hydrolysis supernatant and digests residue；5)
Enzymatic hydrolysis supernatant is heated to 90 DEG C and keeps enzyme deactivation in 0.5 hour, then cools to room temperature standing 2 hours, obtains enzymatic hydrolysis enzyme deactivation
Supernatant；
Step 6: filtering
The enzymatic hydrolysis of self-dissolving supernatant, the ultrasonication supernatant that step 4 obtains and step 5 acquisition that step 3 is obtained
Enzyme deactivation supernatant is mixed, and is stirred evenly, and the ultrafiltration membrane ultrafiltration for being 6000-7000Da with molecular cut off obtains ultrafiltration transmission
Then liquid is diluted with appropriate amount of purified water, then the nanofiltration membrane for being 400-600Da with molecular cut off carries out dialysis concentration, is received
Filter concentration liquid；
Step 7: freeze-drying:
Step 6 nanofiltration concentrate obtained is removed into moisture by the way of freeze-drying and obtains powdered sea cucumber rice noodle roll.
Third object of the present invention is to provide a kind of to be combined endogenous enzymes self-dissolving and ultrasonication to obtain sea
Residue after self-dissolving can be carried out ultrasound by the preparation method of ginseng enteral nutrient solution and its prepared sea cucumber enteral nutrient solution
Wave reprocessing, to effectively avoid waste caused by self-dissolving, proposes the nutritional ingredient as much as possible of sea cucumber intestine
It takes, while entire preparation method does not use any natural constituent, so that prepared sea cucumber enteral nutrient solution is without any non-day
So addition, is effectively ensured the safety and natural sex that it is used, furthermore by Chinese medicine extract and as close possible to sea cucumber intestine whole
The supernatant of nutritional ingredient is mixed, on the one hand avoid during existing whole raw material mixed processing caused by nutrition
It is interfered with each other with medicinal ingredient, is on the other hand also convenient for Chinese medicine extract nutritional and officinal compositions and sea cucumber intestine nutritional ingredient carries out
More nutritive validity is realized in cooperation.
Third object of the present invention is achieved through the following technical solutions: a kind of preparation side of sea cucumber enteral nutrient solution
Method, the preparation method carry out as follows:
Step 1: sea cucumber intestine pre-processes: 1) collecting fresh sea cucumber intestine；2) pure water pre-soaking sea cucumber intestine is used, when pre-soaking
Between 1.5 hours and every 0.5 hour stir 1 time, then by the sea cucumber intestine through pre-soaking repeatedly with pure water impregnate five times, every time
It impregnates 0.5 hour；3) it freezes, the sea cucumber intestine taking-up after pure water is impregnated repeatedly is refrigerated to complete knot at a temperature of being placed in -4 DEG C
Block；4) it thaws: the sea cucumber intestine after freezing being placed in pure water after thawing and continue to impregnate 0.5 hour in pure water, take out drip
Clean transparent sea cucumber intestine is obtained after water；
Step 2: the homogeneous of sea cucumber intestine:
It will be crushed and be slurried using wet process high speed through the pretreated sea cucumber intestine of step 1, and suitable pure water is added, carried out equal
Matter obtains sea cucumber intestine homogenizing fluid；
Step 3: self-dissolving:
The sea cucumber intestine homogenizing fluid that will be obtained through step 2 makes it under the following conditions using the effect of endogenous enzymes contained by sea cucumber intestine
Self-dissolving: placing it in below ultraviolet radiator at 0.4 meter, and control slurries liquid level UV intensity is 100W/ cm2, it irradiates 0.5 hour,
Appropriate natural lemon acid is added, pH value is adjusted to pH5.0-6.5, keeps 4 DEG C of self-dissolvings 10 hours；Product after self-dissolving is placed in horizontal
Screw settling centrifuge, and centrifugation 0.5 hour is carried out with 3500r/min, obtain self-dissolving supernatant and self-dissolving residue；
Step 4: ultrasonication:
1) self-dissolving residue obtained in step 3 is inputted in vacuum ultrasonic reaction kettle；2) it is evacuated to 100Pa, controls kettle
0 DEG C of interior temperature；3) power for adjusting ultrasonic transmitter is 500W, sets ultrasonic wave tranmitting frequency as 25KHz, the pulse period is frequently
15 time/second of rate, 1700 meter per second of acoustic beam, liquid surface sound pressure level are 20 °, start ultrasonic wave and control ultrasonic intensity, make self-dissolving
Residue generates vortex, ultrasonication 2.5 hours, obtains sea cucumber intestine active component extract；4) sea cucumber active constituent is mentioned
Object is taken, in addition tube centrifuge in batches, starting centrifuge starts, and sets revolution as 15000r/min, centrifugation time 1 is small
When, temperature is set as 0 DEG C, and separation obtains ultrasonication supernatant and ultrasonication residue；
Step 5: preparing nutrient solution:
Fructus lycii, ginseng, dark plum and Siraitia grosvenorii are taken, is processed using steam water-boiling method, Chinese medicine extract is made using filtering,
Step 3 self-dissolving supernatant obtained, step 4 ultrasonication supernatant obtained are mixed simultaneously with the Chinese medicine extract
Stir evenly, and the ultrafiltration membrane ultrafiltration for being 6000-7000Da with molecular cut off obtains ultrafiltration permeate, then adjust its pH value 4 to
It between 6, is diluted, then the nanofiltration membrane for being 400-600Da with molecular cut off carries out dialysis concentration, is obtained natural with appropriate amount of purified water
Sea cucumber enteral nutrient solution.
Fourth object of the present invention is to provide a kind of by the progress of endogenous enzymes self-dissolving, ultrasonication and external source enzyme resolving
In conjunction with come obtain sea cucumber enteral nutrient solution preparation method and its prepared sea cucumber enteral nutrient solution, can will be residual after self-dissolving
Object is stayed to carry out ultrasonic wave reprocessing, so that waste caused by endogenous enzymes self-dissolving is effectively avoided, it is then also residual to ultrasonication
Object is stayed to carry out external source enzyme resolving processing, so that waste caused by ultrasonication is further effectively prevented, so that sea cucumber intestine
Nutritional ingredient as much as possible extracted, while entire preparation method does not use any natural constituent, so that made
Without any artificial addition the safety and natural sex that it is used is effectively ensured, furthermore by traditional Chinese medicine extraction in standby sea cucumber enteral nutrient solution
Liquid is mixed with the supernatant as close possible to sea cucumber intestine whole nutritional ingredient, and it is mixed on the one hand to avoid existing whole raw material
It closes nutritional and officinal compositions resulted in treatment process to interfere with each other, is on the other hand also convenient for Chinese medicine extract nutrition and medicinal
Ingredient carries out the realization more nutritive validity that cooperates with sea cucumber intestine nutritional ingredient.
Fourth object of the present invention is achieved through the following technical solutions: a kind of preparation side of sea cucumber enteral nutrient solution
Method, the preparation method carry out as follows:
Step 1: sea cucumber intestine pre-processes: 1) collecting fresh sea cucumber intestine；2) pure water pre-soaking sea cucumber intestine is used, when pre-soaking
Between 1.5 hours and every 0.5 hour stir 1 time, then by the sea cucumber intestine through pre-soaking repeatedly with pure water impregnate five times, every time
It impregnates 0.5 hour；3) it freezes, the sea cucumber intestine taking-up after pure water is impregnated repeatedly is refrigerated to complete knot at a temperature of being placed in -4 DEG C
Block；4) it thaws: the sea cucumber intestine after freezing being placed in pure water after thawing and continue to impregnate 0.5 hour in pure water, take out drip
Clean transparent sea cucumber intestine is obtained after water；
Step 2: the homogeneous of sea cucumber intestine:
It will be crushed and be slurried using wet process high speed through the pretreated sea cucumber intestine of step 1, and suitable pure water is added, carried out equal
Matter obtains sea cucumber intestine homogenizing fluid；
Step 3: self-dissolving:
The sea cucumber intestine homogenizing fluid that will be obtained through step 2 makes it under the following conditions using the effect of endogenous enzymes contained by sea cucumber intestine
Self-dissolving: placing it in below ultraviolet radiator at 0.4 meter, and control slurries liquid level UV intensity is 100W/ cm2, it irradiates 0.5 hour,
Appropriate natural lemon acid is added, pH value is adjusted to pH5.0-6.5, keeps 4 DEG C of self-dissolvings 10 hours；Product after self-dissolving is placed in horizontal
Screw settling centrifuge, and centrifugation 0.5 hour is carried out with 3500r/min, obtain self-dissolving supernatant and self-dissolving residue；
Step 4: ultrasonication:
1) self-dissolving residue obtained in step 3 is inputted in vacuum ultrasonic reaction kettle；2) it is evacuated to 100Pa, controls kettle
0 DEG C of interior temperature；3) power for adjusting ultrasonic transmitter is 500W, sets ultrasonic wave tranmitting frequency as 25KHz, the pulse period is frequently
15 time/second of rate, 1700 meter per second of acoustic beam, liquid surface sound pressure level are 20 °, start ultrasonic wave and control ultrasonic intensity, make self-dissolving
Residue generates vortex, ultrasonication 2.5 hours, obtains sea cucumber intestine active component extract；4) sea cucumber active constituent is mentioned
Object is taken, in addition tube centrifuge in batches, starting centrifuge starts, and sets revolution as 15000r/min, centrifugation time 1 is small
When, temperature is set as 0 DEG C, and separation obtains ultrasonication supernatant and ultrasonication residue；
Step 5: enzymolysis processing；
1) appropriate amount of purified water will be added through the separating obtained ultrasonication residue of step 4, stirs evenly, is heated to 100 DEG C
Kept for 0.5 hour, be sufficiently swollen, 2) it is then cooled to 45-50 DEG C, it adjusts pH value to 6.5-6.7, is added in right amount by bacillus subtilis
Neutral proteinase obtained by the fermented extraction of bacterium, stirs evenly, enzymatic hydrolysis 2.5 hours, 3) 55-60 DEG C is then heated to, adjust pH value
To 6.8-7.0, papain is added, stirs evenly, digest 3.5 hours, be then cooled to 4 DEG C, obtains enzymolysis liquid；4) by enzyme
It solves liquid and utilizes disk plate centrifuge, and separation 30 minutes is carried out with 6000r/min, obtain enzymatic hydrolysis supernatant and digests residue；5)
Enzymatic hydrolysis supernatant is heated to 90 DEG C and keeps enzyme deactivation in 0.5 hour, then cools to room temperature standing 2 hours, obtains enzymatic hydrolysis enzyme deactivation
Supernatant；
Step 6: preparing nutrient solution:
Fructus lycii, ginseng, dark plum and Siraitia grosvenorii are taken, is processed using steam water-boiling method, Chinese medicine extract is made using filtering,
In the enzymatic hydrolysis enzyme deactivation of self-dissolving supernatant, the ultrasonication supernatant that step 4 obtains and step 5 acquisition that step 3 obtains
Clear liquid is mixed and is stirred evenly with the Chinese medicine extract, and to obtain ultrafiltration saturating for the ultrafiltration membrane ultrafiltration for being 6000-7000Da with molecular cut off
Liquid is crossed, its pH value is then adjusted between 4 to 6, is diluted with appropriate amount of purified water, then with molecular cut off is receiving for 400-600Da
Filter membrane carries out dialysis concentration, obtains natural sea cucumber enteral nutrient solution.
The sea cucumber rice noodle roll and sea cucumber enteral nutrient solution preparation method of sea cucumber intestine powder, preparation method thereof and its preparation of the invention and its
The sea cucumber enteral nutrient solution of preparation brings following beneficial technical effect:
1) present invention makes full use of endogenous enzymes to carry out self-dissolving, and selects suitable uitraviolet intensity and irradiation condition, to optimize
The abundant induction of sea cucumber intestine endogenous enzymes is directed to simultaneously to improve the effect of self-dissolving while effectively avoiding external addition
A large amount of nutritional ingredients present in self-dissolving residue are reprocessed, and are further extracted in sea cucumber intestine by means of ultrasonication
Nutritional ingredient, so as to avoid the loss of sea cucumber intestine nutritional ingredient caused by simple self-dissolving, and by endogenous enzymes self-dissolving with
Ultrasonication uses similar temperature, on the one hand avoids nutritional ingredient because of variation caused by temperature change, also avoids locating
Manage waste of time and energy waste；
2) present invention for self-dissolving and when ultrasonication temperature it is single caused by residue in there are still a large amount of nutritional ingredients,
It is handled by means of external source enzyme resolving, passes through 45-50 DEG C of temperature of optimum neutral proteinase and the environmental condition of pH value 6.5-6.7
And successively digested by 55-60 DEG C of temperature of optimum papain and the environmental condition of pH value 6.8-7.0, sufficiently
Make the two action compensating using the difference of both neutral proteinase and papain optimal environmental condition and realize most preferably, keeps away
The loss for having exempted from nutritional ingredient improves the utilization rate of sea cucumber intestine, so that sea cucumber intestine nutritional ingredient as much as possible is retained；
3) in the present invention, the pretreatment of sea cucumber intestine is only by the pre-soaking of pure water, repeatedly immersion, freezing and immersion defrosting
Mode realize the cleaning of sea cucumber intestine, avoid the use of various additives；
4) in the present invention, supernatant obtained in self-dissolving, ultrasonication, each step of external source enzyme resolving is mixed,
Avoid defect with single nutrient component caused by single extracting mode, obtain as close possible to the nutrition of sea cucumber intestine whole at
The sea cucumber rice noodle roll and sea cucumber enteral nutrient solution divided；
5) in the present invention, by fructus lycii, ginseng, dark plum and Siraitia grosvenorii by steam water-boiling method be made Chinese medicine extract, will in
Medicament extracting solution is mixed with the supernatant as close possible to sea cucumber intestine whole nutritional ingredient, is on the one hand avoided existing all former
Nutritional and officinal compositions caused by during material mixed processing interfere with each other, and are on the other hand also convenient for Chinese medicine extract nutrition
The realization more nutritive validity that cooperates is carried out with sea cucumber intestine nutritional ingredient with medicinal ingredient.
Specific embodiment
The preparation method of sea cucumber rice noodle roll and sea cucumber enteral nutrient solution is now described by following embodiment.
Embodiment 1:
In this embodiment, a kind of preparation method of sea cucumber rice noodle roll is provided, which carries out as follows:
Step 1: sea cucumber intestine pre-processes: 1) collecting fresh sea cucumber intestine；2) pure water pre-soaking sea cucumber intestine is used, when pre-soaking
Between 1.5 hours and every 0.5 hour stir 1 time, then by the sea cucumber intestine through pre-soaking repeatedly with pure water impregnate five times, every time
It impregnates 0.5 hour；3) it freezes, the sea cucumber intestine taking-up after pure water is impregnated repeatedly is refrigerated to complete knot at a temperature of being placed in -4 DEG C
Block；4) it thaws: the sea cucumber intestine after freezing being placed in pure water after thawing and continue to impregnate 0.5 hour in pure water, take out drip
Clean transparent sea cucumber intestine is obtained after water；In this step, the pretreatment of sea cucumber intestine only by pure water pre-soaking, repeatedly
Impregnating, freeze and impregnate the mode thawed realizes the cleaning of sea cucumber intestine, avoids the use of various additives.
Step 2: the homogeneous of sea cucumber intestine:
It will be crushed and be slurried using wet process high speed through the pretreated sea cucumber intestine of step 1, and suitable pure water is added, carried out equal
Matter obtains sea cucumber intestine homogenizing fluid；
Step 3: self-dissolving:
The sea cucumber intestine homogenizing fluid that will be obtained through step 2 makes it under the following conditions using the effect of endogenous enzymes contained by sea cucumber intestine
Self-dissolving: placing it in below ultraviolet radiator at 0.4 meter, and control slurries liquid level UV intensity is 100W/ cm2, it irradiates 0.5 hour,
Ultraviolet light sufficiently induces endogenous enzymes, adds appropriate natural lemon acid, pH value is adjusted to pH5.0-6.5,
Keep 4 DEG C of self-dissolvings 10 hours；Product after self-dissolving is placed in horizontal type screw settling centrifuge, and centrifugation 0.5 is carried out with 3500r/min
Hour, obtain self-dissolving supernatant and self-dissolving residue；
Step 4: ultrasonication:
1) self-dissolving residue obtained in step 3 is inputted in vacuum ultrasonic reaction kettle；2) it is evacuated to 100Pa, controls kettle
0 DEG C of interior temperature；3) power for adjusting ultrasonic transmitter is 500W, sets ultrasonic wave tranmitting frequency as 25KHz, the pulse period is frequently
15 time/second of rate, 1700 meter per second of acoustic beam, liquid surface sound pressure level are 20 °, start ultrasonic wave and control ultrasonic intensity, make self-dissolving
Residue generates vortex, ultrasonication 2.5 hours, obtains sea cucumber intestine active component extract；4) sea cucumber active constituent is mentioned
Object is taken, in addition tube centrifuge in batches, starting centrifuge starts, and sets revolution as 15000r/min, centrifugation time 1 is small
When, temperature is set as 0 DEG C, and separation obtains ultrasonication supernatant and ultrasonication residue；
Step 5: hybrid filtering
The self-dissolving supernatant that step 3 obtains is mixed with the ultrasonication supernatant that step 4 obtains, is stirred evenly,
The ultrafiltration membrane ultrafiltration for being 6000-7000Da with molecular cut off obtains ultrafiltration permeate, is then diluted with appropriate amount of purified water, then with cut
Staying molecular weight is that the nanofiltration membrane of 400-600Da carries out dialysis concentration, obtains nanofiltration concentrate；
Step 6: freeze-drying:
Step 5 nanofiltration concentrate obtained is removed into moisture by the way of freeze-drying and obtains powdered sea cucumber rice noodle roll.
Embodiment 2:
In this embodiment, a kind of preparation method of sea cucumber rice noodle roll is provided, which carries out as follows:
Wherein step 1 is same as Example 1 to step 4；
Step 5: enzymolysis processing；
1) appropriate amount of purified water will be added through the separating obtained ultrasonication residue of step 4, stirs evenly, is heated to 100 DEG C
Kept for 0.5 hour, be sufficiently swollen, 2) it is then cooled to 45-50 DEG C, it adjusts pH value to 6.5-6.7, is added in right amount by bacillus subtilis
Neutral proteinase obtained by the fermented extraction of bacterium, stirs evenly, enzymatic hydrolysis 2.5 hours, 3) 55-60 DEG C is then heated to, adjust pH value
To 6.8-7.0, papain is added, stirs evenly, digest 3.5 hours, be then cooled to 4 DEG C, obtains enzymolysis liquid；4) by enzyme
It solves liquid and utilizes disk plate centrifuge, and separation 30 minutes is carried out with 6000r/min, obtain enzymatic hydrolysis supernatant and digests residue；5)
Enzymatic hydrolysis supernatant is heated to 90 DEG C and keeps enzyme deactivation in 0.5 hour, then cools to room temperature standing 2 hours, obtains enzymatic hydrolysis enzyme deactivation
Supernatant；
Step 6: filtering
The enzymatic hydrolysis of self-dissolving supernatant, the ultrasonication supernatant that step 4 obtains and step 5 acquisition that step 3 is obtained
Enzyme deactivation supernatant is mixed, and is stirred evenly, and the ultrafiltration membrane ultrafiltration for being 6000-7000Da with molecular cut off obtains ultrafiltration transmission
Then liquid is diluted with appropriate amount of purified water, then the nanofiltration membrane for being 400-600Da with molecular cut off carries out dialysis concentration, is received
Filter concentration liquid；
Step 7: freeze-drying:
Step 6 nanofiltration concentrate obtained is removed into moisture by the way of freeze-drying and obtains powdered sea cucumber rice noodle roll.
In this embodiment, handled by means of external source enzyme resolving, by 45-50 DEG C of temperature of optimum neutral proteinase and
The environmental condition of pH value 6.5-6.7 and pass through 55-60 DEG C of temperature of optimum papain and the environment of pH value 6.8-7.0
Condition is successively digested, and the difference of both neutral proteinase and papain optimal environmental condition is made full use of to make the two
Action compensating is simultaneously realized most preferably, is avoided the loss of nutritional ingredient, is improved the utilization rate of sea cucumber intestine, so that sea cucumber intestine is as far as possible
More nutritional ingredients are retained.
Embodiment 3:
In this embodiment, a kind of preparation method of sea cucumber enteral nutrient solution is provided, which carries out as follows:
Wherein step 1 is same as Example 1 to step 4；
Step 5: preparing nutrient solution:
Fructus lycii, ginseng, dark plum and Siraitia grosvenorii are taken, is processed using steam water-boiling method, Chinese medicine extract is made using filtering,
Step 3 self-dissolving supernatant obtained, step 4 ultrasonication supernatant obtained are mixed simultaneously with the Chinese medicine extract
Stir evenly, and the ultrafiltration membrane ultrafiltration for being 6000-7000Da with molecular cut off obtains ultrafiltration permeate, then adjust its pH value 4 to
It between 6, is diluted, then the nanofiltration membrane for being 400-600Da with molecular cut off carries out dialysis concentration, is obtained natural with appropriate amount of purified water
Sea cucumber enteral nutrient solution.
In this embodiment, Chinese medicine extract is made by steam water-boiling method in fructus lycii, ginseng, dark plum and Siraitia grosvenorii,
Chinese medicine extract is mixed with the supernatant as close possible to sea cucumber intestine whole nutritional ingredient, is on the one hand avoided existing complete
Nutritional and officinal compositions caused by during portion's raw material mixed processing interfere with each other, and are on the other hand also convenient for Chinese medicine extract
Nutritional and officinal compositions carry out the realization more nutritive validity that cooperates with sea cucumber intestine nutritional ingredient.
Embodiment 4:
In this embodiment, a kind of preparation method of sea cucumber enteral nutrient solution is provided, which carries out as follows:
Wherein step 1 is same as Example 1 to step 4；
Step 5: enzymolysis processing；
1) appropriate amount of purified water will be added through the separating obtained ultrasonication residue of step 4, stirs evenly, is heated to 100 DEG C
Kept for 0.5 hour, be sufficiently swollen, 2) it is then cooled to 45-50 DEG C, it adjusts pH value to 6.5-6.7, is added in right amount by bacillus subtilis
Neutral proteinase obtained by the fermented extraction of bacterium, stirs evenly, enzymatic hydrolysis 2.5 hours, 3) 55-60 DEG C is then heated to, adjust pH value
To 6.8-7.0, papain is added, stirs evenly, digest 3.5 hours, be then cooled to 4 DEG C, obtains enzymolysis liquid；4) by enzyme
It solves liquid and utilizes disk plate centrifuge, and separation 30 minutes is carried out with 6000r/min, obtain enzymatic hydrolysis supernatant and digests residue；5)
Enzymatic hydrolysis supernatant is heated to 90 DEG C and keeps enzyme deactivation in 0.5 hour, then cools to room temperature standing 2 hours, obtains enzymatic hydrolysis enzyme deactivation
Supernatant；
Step 6: preparing nutrient solution:
Fructus lycii, ginseng, dark plum and Siraitia grosvenorii are taken, is processed using steam water-boiling method, Chinese medicine extract is made using filtering,
In the enzymatic hydrolysis enzyme deactivation of self-dissolving supernatant, the ultrasonication supernatant that step 4 obtains and step 5 acquisition that step 3 obtains
Clear liquid is mixed and is stirred evenly with the Chinese medicine extract, and to obtain ultrafiltration saturating for the ultrafiltration membrane ultrafiltration for being 6000-7000Da with molecular cut off
Liquid is crossed, its pH value is then adjusted between 4 to 6, is diluted with appropriate amount of purified water, then with molecular cut off is receiving for 400-600Da
Filter membrane carries out dialysis concentration, obtains natural sea cucumber enteral nutrient solution.
What be will be understood is that the present invention is not limited to about feature or particular structural details explained above
Any aspect.In the case where not departing from the scope of the present invention or spirit, the particular implementation described in above
A wide range of replacement comprehensively can be carried out with constituent element.Therefore, it is acted on, wider range of the invention covers technical etc.
The whole modes of those and these means all combined described in being same as.
Claims (8)
1. a kind of preparation method of sea cucumber rice noodle roll, the preparation method carry out as follows:
Step 1: sea cucumber intestine pre-processes: 1) collecting fresh sea cucumber intestine；2) pure water pre-soaking sea cucumber intestine is used, when pre-soaking
Between 1.5 hours and every 0.5 hour stir 1 time, then by the sea cucumber intestine through pre-soaking repeatedly with pure water impregnate five times, every time
It impregnates 0.5 hour；3) it freezes, the sea cucumber intestine taking-up after pure water is impregnated repeatedly is refrigerated to complete knot at a temperature of being placed in -4 DEG C
Block；4) it thaws: the sea cucumber intestine after freezing being placed in pure water after thawing and continue to impregnate 0.5 hour in pure water, take out drip
Clean transparent sea cucumber intestine is obtained after water；
Step 2: the homogeneous of sea cucumber intestine:
It will be crushed and be slurried using wet process high speed through the pretreated sea cucumber intestine of step 1, and suitable pure water is added, carried out equal
Matter obtains sea cucumber intestine homogenizing fluid；
Step 3: self-dissolving:
The sea cucumber intestine homogenizing fluid that will be obtained through step 2 makes it under the following conditions using the effect of endogenous enzymes contained by sea cucumber intestine
Self-dissolving: placing it in below ultraviolet radiator at 0.4 meter, and control slurries liquid level UV intensity is 100W/cm2, it irradiates 0.5 hour,
Appropriate natural lemon acid is added, pH value is adjusted to pH5.0-6.5, keeps 4 DEG C of self-dissolvings 10 hours；Product after self-dissolving is placed in horizontal
Screw settling centrifuge, and centrifugation 0.5 hour is carried out with 3500r/min, obtain self-dissolving supernatant and self-dissolving residue；
Step 4: ultrasonication:
1) self-dissolving residue obtained in step 3 is inputted in vacuum ultrasonic reaction kettle；2) it is evacuated to 100Pa, controls kettle
0 DEG C of interior temperature；3) power for adjusting ultrasonic transmitter is 500W, sets ultrasonic wave tranmitting frequency as 25KHz, the pulse period is frequently
15 time/second of rate, 1700 meter per second of acoustic beam, liquid surface sound pressure level are 20 °, start ultrasonic wave and control ultrasonic intensity, make self-dissolving
Residue generates vortex, ultrasonication 2.5 hours, obtains sea cucumber intestine active component extract；4) sea cucumber active constituent is mentioned
Object is taken, in addition tube centrifuge in batches, starting centrifuge starts, and sets revolution as 15000r/min, centrifugation time 1 is small
When, temperature is set as 0 DEG C, and separation obtains ultrasonication supernatant and ultrasonication residue；
Step 5: hybrid filtering
The self-dissolving supernatant that step 3 obtains is mixed with the ultrasonication supernatant that step 4 obtains, is stirred evenly,
The ultrafiltration membrane ultrafiltration for being 6000-7000Da with molecular cut off obtains ultrafiltration permeate, is then diluted with appropriate amount of purified water, then with cut
Staying molecular weight is that the nanofiltration membrane of 400-600Da carries out dialysis concentration, obtains nanofiltration concentrate；
Step 6: freeze-drying:
Step 5 nanofiltration concentrate obtained is removed into moisture by the way of freeze-drying and obtains powdered sea cucumber rice noodle roll.
2. prepared by the preparation method of a kind of sea cucumber rice noodle roll, sea cucumber rice noodle roll sea cucumber rice noodle roll according to claim 1.
3. a kind of preparation method of sea cucumber rice noodle roll, the preparation method carry out as follows:
Step 1: sea cucumber intestine pre-processes: 1) collecting fresh sea cucumber intestine；2) pure water pre-soaking sea cucumber intestine is used, when pre-soaking
Between 1.5 hours and every 0.5 hour stir 1 time, then by the sea cucumber intestine through pre-soaking repeatedly with pure water impregnate five times, every time
It impregnates 0.5 hour；3) it freezes, the sea cucumber intestine taking-up after pure water is impregnated repeatedly is refrigerated to complete knot at a temperature of being placed in -4 DEG C
Block；4) it thaws: the sea cucumber intestine after freezing being placed in pure water after thawing and continue to impregnate 0.5 hour in pure water, take out drip
Clean transparent sea cucumber intestine is obtained after water；
Step 2: the homogeneous of sea cucumber intestine:
It will be crushed and be slurried using wet process high speed through the pretreated sea cucumber intestine of step 1, and suitable pure water is added, carried out equal
Matter obtains sea cucumber intestine homogenizing fluid；
Step 3: self-dissolving:
The sea cucumber intestine homogenizing fluid that will be obtained through step 2 makes it under the following conditions using the effect of endogenous enzymes contained by sea cucumber intestine
Self-dissolving: placing it in below ultraviolet radiator at 0.4 meter, and control slurries liquid level UV intensity is 100W/ cm2, it irradiates 0.5 hour,
Appropriate natural lemon acid is added, pH value is adjusted to pH5.0-6.5, keeps 4 DEG C of self-dissolvings 10 hours；Product after self-dissolving is placed in horizontal
Screw settling centrifuge, and centrifugation 0.5 hour is carried out with 3500r/min, obtain self-dissolving supernatant and self-dissolving residue；
Step 4: ultrasonication:
1) self-dissolving residue obtained in step 3 is inputted in vacuum ultrasonic reaction kettle；2) it is evacuated to 100Pa, controls kettle
0 DEG C of interior temperature；3) power for adjusting ultrasonic transmitter is 500W, sets ultrasonic wave tranmitting frequency as 25KHz, the pulse period is frequently
15 time/second of rate, 1700 meter per second of acoustic beam, liquid surface sound pressure level are 20 °, start ultrasonic wave and control ultrasonic intensity, make self-dissolving
Residue generates vortex, ultrasonication 2.5 hours, obtains sea cucumber intestine active component extract；4) sea cucumber active constituent is mentioned
Object is taken, in addition tube centrifuge in batches, starting centrifuge starts, and sets revolution as 15000r/min, centrifugation time 1 is small
When, temperature is set as 0 DEG C, and separation obtains ultrasonication supernatant and ultrasonication residue；
Step 5: enzymolysis processing；
1) appropriate amount of purified water will be added through the separating obtained ultrasonication residue of step 4, stirs evenly, is heated to 100 DEG C
Kept for 0.5 hour, be sufficiently swollen, 2) it is then cooled to 45-50 DEG C, it adjusts pH value to 6.5-6.7, is added in right amount by bacillus subtilis
Neutral proteinase obtained by the fermented extraction of bacterium, stirs evenly, enzymatic hydrolysis 2.5 hours, 3) 55-60 DEG C is then heated to, adjust pH value
To 6.8-7.0, papain is added, stirs evenly, digest 3.5 hours, be then cooled to 4 DEG C, obtains enzymolysis liquid；4) by enzyme
It solves liquid and utilizes disk plate centrifuge, and separation 30 minutes is carried out with 6000r/min, obtain enzymatic hydrolysis supernatant and digests residue；5)
Enzymatic hydrolysis supernatant is heated to 90 DEG C and keeps enzyme deactivation in 0.5 hour, then cools to room temperature standing 2 hours, obtains enzymatic hydrolysis enzyme deactivation
Supernatant；
Step 6: filtering
The enzymatic hydrolysis of self-dissolving supernatant, the ultrasonication supernatant that step 4 obtains and step 5 acquisition that step 3 is obtained
Enzyme deactivation supernatant is mixed, and is stirred evenly, and the ultrafiltration membrane ultrafiltration for being 6000-7000Da with molecular cut off obtains ultrafiltration transmission
Then liquid is diluted with appropriate amount of purified water, then the nanofiltration membrane for being 400-600Da with molecular cut off carries out dialysis concentration, is received
Filter concentration liquid；
Step 7: freeze-drying:
Step 6 nanofiltration concentrate obtained is removed into moisture by the way of freeze-drying and obtains powdered sea cucumber rice noodle roll.
4. prepared by the preparation method of a kind of sea cucumber rice noodle roll, sea cucumber rice noodle roll sea cucumber rice noodle roll according to claim 3.
5. a kind of preparation method of sea cucumber enteral nutrient solution, the preparation method carry out as follows:
Step 1: sea cucumber intestine pre-processes: 1) collecting fresh sea cucumber intestine；2) pure water pre-soaking sea cucumber intestine is used, when pre-soaking
Between 1.5 hours and every 0.5 hour stir 1 time, then by the sea cucumber intestine through pre-soaking repeatedly with pure water impregnate five times, every time
It impregnates 0.5 hour；3) it freezes, the sea cucumber intestine taking-up after pure water is impregnated repeatedly is refrigerated to complete knot at a temperature of being placed in -4 DEG C
Block；4) it thaws: the sea cucumber intestine after freezing being placed in pure water after thawing and continue to impregnate 0.5 hour in pure water, take out drip
Clean transparent sea cucumber intestine is obtained after water；
Step 2: the homogeneous of sea cucumber intestine:
It will be crushed and be slurried using wet process high speed through the pretreated sea cucumber intestine of step 1, and suitable pure water is added, carried out equal
Matter obtains sea cucumber intestine homogenizing fluid；
Step 3: self-dissolving:
The sea cucumber intestine homogenizing fluid that will be obtained through step 2 makes it under the following conditions using the effect of endogenous enzymes contained by sea cucumber intestine
Self-dissolving: placing it in below ultraviolet radiator at 0.4 meter, and control slurries liquid level UV intensity is 100W/ cm2, it irradiates 0.5 hour,
Appropriate natural lemon acid is added, pH value is adjusted to pH5.0-6.5, keeps 4 DEG C of self-dissolvings 10 hours；Product after self-dissolving is placed in horizontal
Screw settling centrifuge, and centrifugation 0.5 hour is carried out with 3500r/min, obtain self-dissolving supernatant and self-dissolving residue；
Step 4: ultrasonication:
1) self-dissolving residue obtained in step 3 is inputted in vacuum ultrasonic reaction kettle；2) it is evacuated to 100Pa, controls kettle
0 DEG C of interior temperature；3) power for adjusting ultrasonic transmitter is 500W, sets ultrasonic wave tranmitting frequency as 25KHz, the pulse period is frequently
15 time/second of rate, 1700 meter per second of acoustic beam, liquid surface sound pressure level are 20 °, start ultrasonic wave and control ultrasonic intensity, make self-dissolving
Residue generates vortex, ultrasonication 2.5 hours, obtains sea cucumber intestine active component extract；4) sea cucumber active constituent is mentioned
Object is taken, in addition tube centrifuge in batches, starting centrifuge starts, and sets revolution as 15000r/min, centrifugation time 1 is small
When, temperature is set as 0 DEG C, and separation obtains ultrasonication supernatant and ultrasonication residue；
Step 5: preparing nutrient solution:
Fructus lycii, ginseng, dark plum and Siraitia grosvenorii are taken, is processed using steam water-boiling method, Chinese medicine extract is made using filtering,
Step 3 self-dissolving supernatant obtained, step 4 ultrasonication supernatant obtained are mixed simultaneously with the Chinese medicine extract
Stir evenly, and the ultrafiltration membrane ultrafiltration for being 6000-7000Da with molecular cut off obtains ultrafiltration permeate, then adjust its pH value 4 to
It between 6, is diluted, then the nanofiltration membrane for being 400-600Da with molecular cut off carries out dialysis concentration, is obtained natural with appropriate amount of purified water
Sea cucumber enteral nutrient solution.
6. a kind of sea cucumber enteral nutrient solution, the preparation side of sea cucumber enteral nutrient solution sea cucumber enteral nutrient solution according to claim 5
It is prepared by method.
7. a kind of preparation method of sea cucumber enteral nutrient solution, the preparation method carry out as follows:
Step 1: sea cucumber intestine pre-processes: 1) collecting fresh sea cucumber intestine；2) pure water pre-soaking sea cucumber intestine is used, when pre-soaking
Between 1.5 hours and every 0.5 hour stir 1 time, then by the sea cucumber intestine through pre-soaking repeatedly with pure water impregnate five times, every time
It impregnates 0.5 hour；3) it freezes, the sea cucumber intestine taking-up after pure water is impregnated repeatedly is refrigerated to complete knot at a temperature of being placed in -4 DEG C
Block；4) it thaws: the sea cucumber intestine after freezing being placed in pure water after thawing and continue to impregnate 0.5 hour in pure water, take out drip
Clean transparent sea cucumber intestine is obtained after water；
Step 2: the homogeneous of sea cucumber intestine:
It will be crushed and be slurried using wet process high speed through the pretreated sea cucumber intestine of step 1, and suitable pure water is added, carried out equal
Matter obtains sea cucumber intestine homogenizing fluid；
Step 3: self-dissolving:
The sea cucumber intestine homogenizing fluid that will be obtained through step 2 makes it under the following conditions using the effect of endogenous enzymes contained by sea cucumber intestine
Self-dissolving: placing it in below ultraviolet radiator at 0.4 meter, and control slurries liquid level UV intensity is 100W/ cm2, it irradiates 0.5 hour,
Appropriate natural lemon acid is added, pH value is adjusted to pH5.0-6.5, keeps 4 DEG C of self-dissolvings 10 hours；Product after self-dissolving is placed in horizontal
Screw settling centrifuge, and centrifugation 0.5 hour is carried out with 3500r/min, obtain self-dissolving supernatant and self-dissolving residue；
Step 4: ultrasonication:
1) self-dissolving residue obtained in step 3 is inputted in vacuum ultrasonic reaction kettle；2) it is evacuated to 100Pa, controls kettle
0 DEG C of interior temperature；3) power for adjusting ultrasonic transmitter is 500W, sets ultrasonic wave tranmitting frequency as 25KHz, the pulse period is frequently
15 time/second of rate, 1700 meter per second of acoustic beam, liquid surface sound pressure level are 20 °, start ultrasonic wave and control ultrasonic intensity, make self-dissolving
Residue generates vortex, ultrasonication 2.5 hours, obtains sea cucumber intestine active component extract；4) sea cucumber active constituent is mentioned
Object is taken, in addition tube centrifuge in batches, starting centrifuge starts, and sets revolution as 15000r/min, centrifugation time 1 is small
When, temperature is set as 0 DEG C, and separation obtains ultrasonication supernatant and ultrasonication residue；
Step 5: enzymolysis processing；
1) appropriate amount of purified water will be added through the separating obtained ultrasonication residue of step 4, stirs evenly, is heated to 100 DEG C
Kept for 0.5 hour, be sufficiently swollen, 2) it is then cooled to 45-50 DEG C, it adjusts pH value to 6.5-6.7, is added in right amount by bacillus subtilis
Neutral proteinase obtained by the fermented extraction of bacterium, stirs evenly, enzymatic hydrolysis 2.5 hours, 3) 55-60 DEG C is then heated to, adjust pH value
To 6.8-7.0, papain is added, stirs evenly, digest 3.5 hours, be then cooled to 4 DEG C, obtains enzymolysis liquid；4) by enzyme
It solves liquid and utilizes disk plate centrifuge, and separation 30 minutes is carried out with 6000r/min, obtain enzymatic hydrolysis supernatant and digests residue；5)
Enzymatic hydrolysis supernatant is heated to 90 DEG C and keeps enzyme deactivation in 0.5 hour, then cools to room temperature standing 2 hours, obtains enzymatic hydrolysis enzyme deactivation
Supernatant；
Step 6: preparing nutrient solution:
Fructus lycii, ginseng, dark plum and Siraitia grosvenorii are taken, is processed using steam water-boiling method, Chinese medicine extract is made using filtering,
In the enzymatic hydrolysis enzyme deactivation of self-dissolving supernatant, the ultrasonication supernatant that step 4 obtains and step 5 acquisition that step 3 obtains
Clear liquid is mixed and is stirred evenly with the Chinese medicine extract, and to obtain ultrafiltration saturating for the ultrafiltration membrane ultrafiltration for being 6000-7000Da with molecular cut off
Liquid is crossed, its pH value is then adjusted between 4 to 6, is diluted with appropriate amount of purified water, then with molecular cut off is receiving for 400-600Da
Filter membrane carries out dialysis concentration, obtains natural sea cucumber enteral nutrient solution.
8. a kind of sea cucumber enteral nutrient solution, the preparation side of sea cucumber enteral nutrient solution sea cucumber enteral nutrient solution according to claim 7
It is prepared by method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810254831.XA CN110353197A (en) | 2018-03-26 | 2018-03-26 | Sea cucumber rice noodle roll and sea cucumber enteral nutrient solution and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810254831.XA CN110353197A (en) | 2018-03-26 | 2018-03-26 | Sea cucumber rice noodle roll and sea cucumber enteral nutrient solution and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110353197A true CN110353197A (en) | 2019-10-22 |
Family
ID=68213050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810254831.XA Pending CN110353197A (en) | 2018-03-26 | 2018-03-26 | Sea cucumber rice noodle roll and sea cucumber enteral nutrient solution and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110353197A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112106947A (en) * | 2020-09-25 | 2020-12-22 | 荣成市慧海创达生物科技有限公司 | Sea cucumber jelly, preparation method thereof and application of sea cucumber jelly as health-care product |
| CN113004384A (en) * | 2021-02-20 | 2021-06-22 | 中国海洋大学 | Preparation method and application of sea cucumber intestine bone-promoting peptide |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828744A (en) * | 2010-05-13 | 2010-09-15 | 大连蓝德奥科技有限公司 | Anti-fatigue total nutrition high activity seacucumber drinking liquid capable of improving immunity and preparation method thereof |
| WO2012079310A1 (en) * | 2010-12-14 | 2012-06-21 | 大连海晏堂生物有限公司 | Sea cucumber compound preparation and preparation method thereof |
| CN103141872A (en) * | 2011-12-22 | 2013-06-12 | 大连工业大学 | Method for preparing active substance by sea cucumber intestine autolysis |
| CN103859368A (en) * | 2012-12-10 | 2014-06-18 | 威海新异生物科技有限公司 | Sea cucumber beverage and production method thereof |
| CN105558700A (en) * | 2016-01-30 | 2016-05-11 | 威海新异生物科技有限公司 | Preparation method of sea cucumber biological activity drink |
| CN106262941A (en) * | 2016-08-17 | 2017-01-04 | 吴招杰 | Compound trepang intestinal polypeptide powder and preparation method thereof |
| CN106665790A (en) * | 2017-03-10 | 2017-05-17 | 上海新成食品有限公司 | Treatment method of non-dry sea cucumber for preventing autolysis |
-
2018
- 2018-03-26 CN CN201810254831.XA patent/CN110353197A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828744A (en) * | 2010-05-13 | 2010-09-15 | 大连蓝德奥科技有限公司 | Anti-fatigue total nutrition high activity seacucumber drinking liquid capable of improving immunity and preparation method thereof |
| WO2012079310A1 (en) * | 2010-12-14 | 2012-06-21 | 大连海晏堂生物有限公司 | Sea cucumber compound preparation and preparation method thereof |
| CN103141872A (en) * | 2011-12-22 | 2013-06-12 | 大连工业大学 | Method for preparing active substance by sea cucumber intestine autolysis |
| CN103859368A (en) * | 2012-12-10 | 2014-06-18 | 威海新异生物科技有限公司 | Sea cucumber beverage and production method thereof |
| CN105558700A (en) * | 2016-01-30 | 2016-05-11 | 威海新异生物科技有限公司 | Preparation method of sea cucumber biological activity drink |
| CN106262941A (en) * | 2016-08-17 | 2017-01-04 | 吴招杰 | Compound trepang intestinal polypeptide powder and preparation method thereof |
| CN106665790A (en) * | 2017-03-10 | 2017-05-17 | 上海新成食品有限公司 | Treatment method of non-dry sea cucumber for preventing autolysis |
Non-Patent Citations (1)
| Title |
|---|
| 侯英雪: "酶解海参肠口服液的研究", 《食品研究与开发》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112106947A (en) * | 2020-09-25 | 2020-12-22 | 荣成市慧海创达生物科技有限公司 | Sea cucumber jelly, preparation method thereof and application of sea cucumber jelly as health-care product |
| CN113004384A (en) * | 2021-02-20 | 2021-06-22 | 中国海洋大学 | Preparation method and application of sea cucumber intestine bone-promoting peptide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103719926B (en) | A kind of preparation method of sea cucumber intestine oral liquid | |
| CN103141895B (en) | Natural marine animal peptide beautifying drink | |
| CN109170469A (en) | A kind of Isin glue collagen peptide solid beverage and preparation method thereof | |
| CN106174436A (en) | A kind of preparation method of complex enzyme hydrolysis fishbone hormone | |
| CN101422205A (en) | Preparation method of sea cucumber intestine polypeptide capsule | |
| US20190090521A1 (en) | Method of ecological utilization of silver carp | |
| CN105326035A (en) | Production method of low-salt oyster polypeptide and oligosaccharide nutrition powder | |
| CN101396105B (en) | Manufacture method of essence powder using sea cucumber intestine | |
| CN103088097A (en) | Preparation method of matreel active peptide | |
| CN102406048B (en) | Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution | |
| CN105925649B (en) | Preparation method of low-molecular-weight degreased squid protein functional active peptide | |
| CN102766670A (en) | Preparation method of oyster polypeptide powder | |
| CN109943612B (en) | Method for producing high-activity minced fillet and product antifreeze agent by enzymolysis of silver carp scales | |
| CN109486890A (en) | A kind of technique preparing Fish protein hybrid peptide from crude fish protein | |
| CN102793138A (en) | Shellfish flavor peptide and preparation method thereof | |
| CN105341933A (en) | Trepang and oyster enzymolysis oral liquid and preparation method thereof | |
| CN106174597A (en) | A kind of compound nutritional base stock powder and preparation method thereof | |
| CN110353197A (en) | Sea cucumber rice noodle roll and sea cucumber enteral nutrient solution and preparation method thereof | |
| CN109486887A (en) | It is a kind of ultrasound and enzymatic hydrolysis coordinate system for oyster polypeptide method | |
| CN105724995B (en) | Clarify the preparation method of Yam beverage | |
| CN108315161A (en) | A kind of lycium ruthenicum wine and preparation method thereof | |
| CN102511890A (en) | Jellyfish beverage | |
| CN103621929A (en) | Method for preparing sugar-free and smell-free pumpkin powder | |
| CN1185960C (en) | Process for preparing micropeptide bone paste | |
| CN106367461A (en) | Method for extracting low molecular weight active collagen peptide from pigskins |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20191022 |
|
| WD01 | Invention patent application deemed withdrawn after publication |