CN102551112B - Rapid preparation method of concentrate and powder of Antarctic krill on board - Google Patents
Rapid preparation method of concentrate and powder of Antarctic krill on board Download PDFInfo
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- CN102551112B CN102551112B CN201010586800.8A CN201010586800A CN102551112B CN 102551112 B CN102551112 B CN 102551112B CN 201010586800 A CN201010586800 A CN 201010586800A CN 102551112 B CN102551112 B CN 102551112B
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- 241000239366 Euphausiacea Species 0.000 title claims abstract description 88
- 239000012141 concentrate Substances 0.000 title claims abstract description 41
- 239000000843 powder Substances 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 108091005804 Peptidases Proteins 0.000 claims abstract description 43
- 239000004365 Protease Substances 0.000 claims abstract description 43
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 43
- 238000000034 method Methods 0.000 claims abstract description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- 238000012545 processing Methods 0.000 claims abstract description 9
- 239000007787 solid Substances 0.000 claims abstract 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 99
- 230000008569 process Effects 0.000 claims description 35
- 239000000758 substrate Substances 0.000 claims description 33
- 238000001035 drying Methods 0.000 claims description 17
- 230000008719 thickening Effects 0.000 claims description 17
- 239000002994 raw material Substances 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims 1
- 239000002002 slurry Substances 0.000 abstract description 32
- 102000004190 Enzymes Human genes 0.000 abstract description 6
- 108090000790 Enzymes Proteins 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 241000239370 Euphausia superba Species 0.000 abstract description 2
- 208000035404 Autolysis Diseases 0.000 abstract 3
- 206010057248 Cell death Diseases 0.000 abstract 3
- 230000028043 self proteolysis Effects 0.000 abstract 3
- 239000013543 active substance Substances 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 230000005855 radiation Effects 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
- 239000000725 suspension Substances 0.000 description 31
- 230000001678 irradiating effect Effects 0.000 description 29
- 239000007921 spray Substances 0.000 description 15
- 241000238557 Decapoda Species 0.000 description 7
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 229910052731 fluorine Inorganic materials 0.000 description 4
- 239000011737 fluorine Substances 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 238000005265 energy consumption Methods 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000012190 activator Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
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- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 1
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- MBLBDJOUHNCFQT-LXGUWJNJSA-N aldehydo-N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 description 1
- 229940022405 astaxanthin Drugs 0.000 description 1
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Abstract
The invention discloses a rapid preparation method of concentrate and powder of Antarctic krill (Euphausia superba) on board, comprising the following steps: draining and squeezing to crush the caught fresh Antarctic krill; subjecting the broken materials to ultraviolet radiation and low temperature autolysis; adding water into the slurry after low temperature autolysis, adjusting the pH after ultrasonic processing, and adding low temperature protease to perform exogenous enzyme enzymolysis; sieving the zymolyte, vacuum concentrating the filtered solution optionally at low temperature to obtain the concentrate of Antarctic krill with 40-70% of solid content, or spray-drying the filtered solution to obtain the powder of Antarctic krill. The method of the invention is especially suitable for operation to the caught fresh Antarctic krill on catcher vessels, and has the advantages of simple operation and high efficiency. The method of the invention utilizes the high autolysis ability of fresh Antarctic krill to reduce the usage amount of exogenous enzyme so as to reduce the production cost. The extraction method of the invention has mild conditions and can retain intrinsic physiologically active substances of the Antarctic krill to endow the product with trophism and functionality.
Description
Technical field
The present invention relates to utilize fast preparation method on the ship of krill concentrate and powder.
Background technology
Krill (EupHausia superba) is single one of living resources of planting maximum on the earth, the estimated value of its standing crop is about 4～1,500,000,000 tons, ripe shrimp annual production is 3～500,000,000 tons, and year can reach 100,000,000 tons of left and right by quantity of the catch, forms huge potential fishery resources.In recent years, along with the exhaustion gradually of worldwide traditional fishery resource, and the proposition of 200-nautical-mile exclusive economic zone, make krill resource huge in antarctic waters receive the concern of some deep-sea fishing developed countries.China also lists krill resource in one of main exploitation kind of Development for Distant Water Fishery from now on.
Krill is nutritious, also contains bioactivator.Research shows, in butt krill, contain 60～80% protein (wherein essential amino acids content account for total amino acid 40～50%), 5～20% grease (wherein unrighted acid account for TFA 35～50%), 7～9% carbohydrate (being mainly chitin), and approximately 0.01% astaxanthin.Therefore, krill can be used as the important high-quality living resources of the mankind and is developed, utilizes.
That the Antarctic Continent is located in is remote, krill needs long period transportation, preservation after fishing for.In krill shell, contain the fluorine of high concentration, in preserving process, can, to shrimp migration, pollute shrimp, affect the security of shrimp protein product.Meanwhile, in krill, be rich in polyunsaturated fatty acid, easily putrid and deteriorated.Therefore, krill is fished for original quality need be rapidly freezing, that ultralow temperature is preserved guarantee shrimp.In krill, contain more than 70% moisture, under wet basis state, low-temperature preservation is transported, and has greatly improved the transport preservation cost of krill, becomes the bottleneck of restriction krill resources development and utilization.Solve the problem of krill transport, preservation difficulty, best bet is exactly after fishing for, the shell meat of krill to be separated, and krill meat is prepared into complete nutrition ingredients concentrate or dry powder, so both solved the problem of fluorine migration in shell, reduce again freight volume, reduced the high energy consumption problem that low-temperature transport brings.At present, still lack the processing on ship preparation method of krill meat complete nutrition ingredients concentrate and dry powder.
Summary of the invention
The present invention aims to provide the fast preparation method of a kind of krill concentrate and powder, is the simple and easy method operating on a kind of especially applicable ship.
In order to achieve the above object, the invention discloses fast preparation method on the ship of a kind of krill concentrate and powder, in turn include the following steps:
Step1, raw material processing: the fresh krill of fishing for is drained to rear squeezing broken, obtain broken thing;
Step2, low temperature self-dissolving: use the ultraviolet ray of 20～40 watts of power, wavelength 200～300 nanometers to irradiate described broken thing 10～30 minutes apart from 0.5～1 meter; Afterwards 0～4 DEG C (fishing for area surroundings temperature) lower self-dissolving 1～5 hour;
Step3, ultrasonic assisted cryogenic external source enzymolysis: to the water that adds 1～3 times of volume in described broken thing, with 300～800 watts of ultrasonic waves (wavelength 200～400 nanometers) processing 5～15 minutes, regulate pH value to 7.5～8.5 of described homogenate, in described homogenate, add 9～10 × 10
5the low-temperature protease of U/kg substrate, at 10～20 DEG C, enzymolysis obtains zymolyte for 1～4 hour;
Step4, zymolyte is crossed to 120～200 objects sieves; Filtered solution or after cryogenic vacuum is concentrated krill concentrate, or after spray-dried krill dry powder.
Under optimal way, Novi's letter wave power low-temperature protease that low-temperature protease selects letter (China) Investment Co., Ltd of Novi to produce.And in above-mentioned Step2, regulate the pH value of homogenate, preferentially select HCl or the NaOH of 1M.
In Step4, to carry out cryogenic vacuum with 20～25 DEG C of thickening temperatures, vacuum 75～90kPa concentrated for filtered solution, makes concentrate; Or filtered solution is taking drying tower EAT as 150～180 DEG C, delivery temperature as 75～95 DEG C are sprayed dryly, makes dry powder.The raw material of nutritional labeling transported land back and also can be used as extracting by concentrate and dry powder.
In addition in above-mentioned Step1, " draining " object is that the water that fresh and alive krill is adhered to is removed, and drains rear shrimp itself and still keeps fresh shrimp state." squeezing is broken " object is that the nutrients mass-energy in krill body is discharged more fully, but does not destroy the integrality of shell as far as possible, reduces the release of fluorine in shell.And the object that ultraviolet ray is irradiated is the self-dissolving ability in order to promote krill; Similar, ultrasonic wave processing intent is intended to promote enzymolysis ability.
The invention provides fast preparation method on the ship of a kind of krill concentrate and powder.The fresh krill of fishing for is drained to rear squeezing broken, broken thing is low temperature self-dissolving after ultraviolet irradiation.In the slurries after low temperature self-dissolving, add water, and carry out after ultrasonic processing, regulate pH value, add low-temperature protease to carry out external source enzyme resolving.Zymolyte is sieved, after filtered solution is spray-dried, obtain krill dry powder.The present invention is applied to self-dissolving technology, ultrasonic technique, cold induced proteins zymotechnic fast preparation method on the ship of krill concentrate and powder first simultaneously, and leaching process is simple to operate, and efficiency is high.Due to the strong self-dissolving ability of having utilized fresh and alive krill self to possess, reduce the consumption of exogenous enzymes, reduce production cost.It is low-temperature protease that the present invention extracts exogenous enzymes used, and applicable elements gentleness, and original physiological activator can preserve to greatest extent krill fat in reducing energy consumption in, make product have trophism and functional concurrently.The present invention has the following advantages:
1, the operating process the present invention relates to is simple, does not need complicated equipment, is adapted at carrying out on dredger.
2, the present invention utilizes the strong self-dissolving ability that krill self exists, and can reduce exogenous enzymes consumption, reduces production costs.
3, the present invention's preparation for krill concentrate and powder by low-temperature protease first, whole operating process temperature is all lower, in reducing energy consumption, can preserve to greatest extent krill fat in more original physiological activators.
4, the present invention's preparation for krill concentrate and powder by squeezing crushing technology first, this technology is light to the destructiveness of krill shell, reduces to greatest extent the release of fluorine in shrimp shell.
Detailed description of the invention
Fast preparation method on the ship of a kind of krill concentrate of the present invention and powder, comprises the following steps:
1, raw material processing: the fresh krill of fishing for is drained to rear squeezing broken, obtain broken thing.
2, low temperature self-dissolving: use 200～300 nanometer ultraviolet rays of 20～40 watts of power, 0.5～1 meter of distance to irradiate described broken thing 10～30 minutes; Afterwards 0～4 DEG C (fishing for area surroundings temperature) lower self-dissolving 1～5 hour.
3, ultrasonic assisted cryogenic external source enzymolysis: to the water that adds 1～3 times of volume in described broken thing, with 300～800 watts of ultrasonic waves (200～400 nanometer) processing 5～15 minutes, pH value to 7.5～8.5 that regulate described broken thing suspension with the HCl of 1M or NaOH add 9～10 × 10 in described homogenate
5the low-temperature protease of U/kg substrate, at 10～20 DEG C, enzymolysis obtains zymolyte for 1～4 hour.Low-temperature protease is selected Novi's letter wave power low-temperature protease.
4, zymolyte is crossed to 120～200 objects sieves, filtered solution or after cryogenic vacuum is concentrated krill concentrate, or after spray-dried krill dry powder.
Embodiment 1
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 20 watts, and 200 nanometer ultraviolet rays that distance is 0.5 meter descend self-dissolving 1 hour 0 DEG C (fishing for area surroundings temperature) after irradiating 10 minutes ultraviolet irradiations of broken thing.To the water of 1 times of volume in the slurries after low temperature self-dissolving, and process after 5 minutes with 300 watts of ultrasonic waves (200 nanometer), the pH value to 7.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 1 hour at 10 DEG C.Zymolyte is crossed to 120 object sieves, and filtered solution, through carrying out Vacuum Concentration with 20 DEG C of thickening temperatures, vacuum 75kPa, makes concentrate.
Embodiment 2
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 20 watts, and 200 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 3 hours 2 DEG C (fishing for area surroundings temperature) after irradiating 10 minutes ultraviolet irradiations of described broken things.To the water of 1 times of volume in the slurries after low temperature self-dissolving, and process after 10 minutes with 500 watts of ultrasonic waves (300 nanometer), the pH value to 8.0 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 1 × 10 in described homogenate
6the low-temperature protease of U/kg substrate enzymolysis 3 hours at 10 DEG C.Zymolyte is crossed to 160 object sieves, and filtered solution, through carrying out Vacuum Concentration with 25 DEG C of thickening temperatures, vacuum 75kPa, makes concentrate.
Embodiment 3
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 20 watts, and 200 nanometer ultraviolet rays that distance is 0.75 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 20 minutes ultraviolet irradiations of described broken things.To the water of 3 times of volumes in the slurries after low temperature self-dissolving, and process after 10 minutes with 500 watts of ultrasonic waves (400 nanometer), the pH value to 8.0 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 1 hour at 20 DEG C.Zymolyte is crossed to 160 object sieves, and filtered solution, through carrying out Vacuum Concentration with 20 DEG C of thickening temperatures, vacuum 80kPa, makes concentrate.
Embodiment 4
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 20 watts, and 250 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 30 minutes ultraviolet irradiations of described broken things.To the water of 2 times of volumes in the slurries after low temperature self-dissolving, and process after 10 minutes with 800 watts of ultrasonic waves (200 nanometer), the pH value to 7.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 1 × 10 in described homogenate
6the low-temperature protease of U/kg substrate enzymolysis 4 hours at 10 DEG C.Zymolyte is crossed to 200 object sieves, and filtered solution, through carrying out Vacuum Concentration with 25 DEG C of thickening temperatures, vacuum 85kPa, makes concentrate.
Embodiment 5
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 20 watts, and 250 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 30 minutes ultraviolet irradiations of described broken things.To the water of 3 times of volumes in the slurries after low temperature self-dissolving, and process after 15 minutes with 800 watts of ultrasonic waves (300 nanometer), the pH value to 8.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 4 hours at 20 DEG C.Zymolyte is crossed to 200 object sieves, and filtered solution, through carrying out Vacuum Concentration with 25 DEG C of thickening temperatures, vacuum 90kPa, makes concentrate.
Embodiment 6
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 3 watts, and 250 nanometer ultraviolet rays that distance is 0.5 meter descend self-dissolving 1 hour 0 DEG C (fishing for area surroundings temperature) after irradiating 10 minutes ultraviolet irradiations of described broken things.To the water of 1 times of volume in the slurries after low temperature self-dissolving, and process after 5 minutes with 300 watts of ultrasonic waves (400 nanometer), the pH value to 7.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 1 hour at 10 DEG C.Zymolyte is crossed to 120 object sieves, and filtered solution, through carrying out Vacuum Concentration with 20 DEG C of thickening temperatures, vacuum 75kPa, makes concentrate.
Embodiment 7
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 30 watts, and 300 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 3 hours 2 DEG C (fishing for area surroundings temperature) after irradiating 10 minutes ultraviolet irradiations of described broken things.To the water of 1 times of volume in the slurries after low temperature self-dissolving, and process after 10 minutes with 500 watts of ultrasonic waves (200 nanometer), the pH value to 8.0 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 1 × 10 in described homogenate
6the low-temperature protease of U/kg substrate enzymolysis 3 hours at 10 DEG C.Zymolyte is crossed to 160 object sieves, and filtered solution, through carrying out Vacuum Concentration with 25 DEG C of thickening temperatures, vacuum 75kPa, makes concentrate.
Embodiment 8
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 30 watts, and 300 nanometer ultraviolet rays that distance is 0.75 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 20 minutes ultraviolet irradiations of described broken things.To the water of 3 times of volumes in the slurries after low temperature self-dissolving, and process after 10 minutes with 500 watts of ultrasonic waves (300 nanometer), the pH value to 8.0 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 1 hour at 20 DEG C.Zymolyte is crossed to 160 object sieves, and filtered solution, through carrying out Vacuum Concentration with 20 DEG C of thickening temperatures, vacuum 80kPa, makes concentrate.
Embodiment 9
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 30 watts, 300 nanometer ultraviolet rays that distance is 1 meter irradiate the ultraviolet ray in 30 minutes of described broken thing irradiate after 4 DEG C (fishing for area surroundings temperature) lower self-dissolving 5 hours.To the water of 2 times of volumes in the slurries after low temperature self-dissolving, and process after 10 minutes with 800 watts of ultrasonic waves (400 nanometer), the pH value to 7.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 1 × 10 in described homogenate
6the low-temperature protease of U/kg substrate enzymolysis 4 hours at 10 DEG C.Zymolyte is crossed to 200 object sieves, and filtered solution, through carrying out Vacuum Concentration with 25 DEG C of thickening temperatures, vacuum 85kPa, makes concentrate.
Embodiment 10
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 30 watts, and 200 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 30 minutes ultraviolet irradiations of described broken things.To the water of 3 times of volumes in the slurries after low temperature self-dissolving, and process after 15 minutes with 800 watts of ultrasonic waves (350 nanometer), the pH value to 8.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 4 hours at 20 DEG C.Zymolyte is crossed to 200 object sieves, and filtered solution, through carrying out Vacuum Concentration with 25 DEG C of thickening temperatures, vacuum 90kPa, makes concentrate.
Embodiment 11
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 40 watts, and 200 nanometer ultraviolet rays that distance is 0.5 meter descend self-dissolving 1 hour 0 DEG C (fishing for area surroundings temperature) after irradiating 10 minutes ultraviolet irradiations of described broken things.To the water of 1 times of volume in the slurries after low temperature self-dissolving, and process after 5 minutes with 300 watts of ultrasonic waves (200 nanometer), the pH value to 7.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 1 hour at 10 DEG C.Zymolyte is crossed to 120 object sieves, and filtered solution, through carrying out Vacuum Concentration with 20 DEG C of thickening temperatures, vacuum 75kPa, makes concentrate.
Embodiment 12
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 40 watts, and 200 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 3 hours 2 DEG C (fishing for area surroundings temperature) after irradiating 10 minutes ultraviolet irradiations of described broken things.To the water of 1 times of volume in the slurries after low temperature self-dissolving, and process after 10 minutes with 500 watts of ultrasonic waves (250 nanometer), the pH value to 8.0 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 1 × 10 in described homogenate
6the low-temperature protease of U/kg substrate enzymolysis 3 hours at 10 DEG C.Zymolyte is crossed to 160 object sieves, and filtered solution, through carrying out Vacuum Concentration with 25 DEG C of thickening temperatures, vacuum 75kPa, makes concentrate.
Embodiment 13
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 40 watts, and 200 nanometer ultraviolet rays that distance is 0.75 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 20 minutes ultraviolet irradiations of described broken things.To the water of 3 times of volumes in the slurries after low temperature self-dissolving, and process after 10 minutes with 500 watts of ultrasonic waves (400 nanometer), the pH value to 8.0 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 1 hour at 20 DEG C.Zymolyte is crossed to 160 object sieves, and filtered solution, through carrying out Vacuum Concentration with 20 DEG C of thickening temperatures, vacuum 80kPa, makes concentrate.
Embodiment 14
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 40 watts, and 250 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 30 minutes ultraviolet irradiations of described broken things.To the water of 2 times of volumes in the slurries after low temperature self-dissolving, and process after 10 minutes with 800 watts of ultrasonic waves (200 nanometer), the pH value to 7.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 1 × 10 in described homogenate
6the low-temperature protease of U/kg substrate enzymolysis 4 hours at 10 DEG C.Zymolyte is crossed to 200 object sieves, and filtered solution, through carrying out Vacuum Concentration with 25 DEG C of thickening temperatures, vacuum 85kPa, makes concentrate.
Embodiment 15
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 40 watts, and 250 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 30 minutes ultraviolet irradiations of described broken things.To the water of 3 times of volumes in the slurries after low temperature self-dissolving, and process after 15 minutes with 800 watts of ultrasonic waves (300 nanometer), the pH value to 8.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 4 hours at 20 DEG C.Zymolyte is crossed to 200 object sieves, and filtered solution, through carrying out Vacuum Concentration with 25 DEG C of thickening temperatures, vacuum 90kPa, makes concentrate.
Embodiment 16
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 20 watts, and 250 nanometer ultraviolet rays that distance is 0.5 meter descend self-dissolving 1 hour 0 DEG C (fishing for area surroundings temperature) after irradiating 10 minutes ultraviolet irradiations of described broken things.To the water of 1 times of volume in the slurries after low temperature self-dissolving, and process after 5 minutes with 300 watts of ultrasonic waves (400 nanometer), the pH value to 7.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 1 hour at 10 DEG C.Zymolyte is crossed to 120 objects sieves, and filtered solution, through taking drying tower EAT as 150 DEG C, delivery temperature as 75 DEG C sprays and are dried, makes dry powder.
Embodiment 17
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 20 watts, and 300 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 3 hours 2 DEG C (fishing for area surroundings temperature) after irradiating 10 minutes ultraviolet irradiations of described broken things.To the water of 1 times of volume in the slurries after low temperature self-dissolving, and process after 10 minutes with 500 watts of ultrasonic waves (200 nanometer), the pH value to 8.0 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 1 × 10 in described homogenate
6the low-temperature protease of U/kg substrate enzymolysis 3 hours at 10 DEG C.Zymolyte is crossed to 160 objects sieves, and filtered solution, through taking drying tower EAT as 150 DEG C, delivery temperature as 95 DEG C sprays and are dried, makes dry powder.
Embodiment 18
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 20 watts, and 300 nanometer ultraviolet rays that distance is 0.75 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 20 minutes ultraviolet irradiations of described broken things.To the water of 3 times of volumes in the slurries after low temperature self-dissolving, and process after 10 minutes with 500 watts of ultrasonic waves (300 nanometer), the pH value to 8.0 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 1 hour at 20 DEG C.Zymolyte is crossed to 160 objects sieves, and filtered solution, through taking drying tower EAT as 170 DEG C, delivery temperature as 85 DEG C sprays and are dried, makes dry powder.
Embodiment 19
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 20 watts, and 300 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 30 minutes ultraviolet irradiations of described broken things.To the water of 2 times of volumes in the slurries after low temperature self-dissolving, and process after 10 minutes with 800 watts of ultrasonic waves (400 nanometer), the pH value to 7.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 1 × 10 in described homogenate
6the low-temperature protease of U/kg substrate enzymolysis 4 hours at 10 DEG C.Zymolyte is crossed to 200 objects sieves, and filtered solution, through taking drying tower EAT as 180 DEG C, delivery temperature as 75 DEG C sprays and are dried, makes dry powder.
Embodiment 20
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 20 watts, and 250 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 30 minutes ultraviolet irradiations of described broken things.To the water of 3 times of volumes in the slurries after low temperature self-dissolving, and process after 15 minutes with 800 watts of ultrasonic waves (350 nanometer), the pH value to 8.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 4 hours at 20 DEG C.Zymolyte is crossed to 200 objects sieves, and filtered solution, through taking drying tower EAT as 180 DEG C, delivery temperature as 95 DEG C sprays and are dried, makes dry powder.
Embodiment 21
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 3 watts, and 200 nanometer ultraviolet rays that distance is 0.5 meter descend self-dissolving 1 hour 0 DEG C (fishing for area surroundings temperature) after irradiating 10 minutes ultraviolet irradiations of described broken things.To the water of 1 times of volume in the slurries after low temperature self-dissolving, and process after 5 minutes with 300 watts of ultrasonic waves (200 nanometer), the pH value to 7.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 1 hour at 10 DEG C.Zymolyte is crossed to 120 objects sieves, and filtered solution, through taking drying tower EAT as 150 DEG C, delivery temperature as 75 DEG C sprays and are dried, makes dry powder.
Embodiment 22
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 30 watts, and 200 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 3 hours 2 DEG C (fishing for area surroundings temperature) after irradiating 10 minutes ultraviolet irradiations of described broken things.To the water of 1 times of volume in the slurries after low temperature self-dissolving, and process after 10 minutes with 500 watts of ultrasonic waves (300 nanometer), the pH value to 8.0 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 1 × 10 in described homogenate
6the low-temperature protease of U/kg substrate enzymolysis 3 hours at 10 DEG C.Zymolyte is crossed to 160 objects sieves, and filtered solution, through taking drying tower EAT as 150 DEG C, delivery temperature as 95 DEG C sprays and are dried, makes dry powder.
Embodiment 23
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 30 watts, and 200 nanometer ultraviolet rays that distance is 0.75 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 20 minutes ultraviolet irradiations of described broken things.To the water of 3 times of volumes in the slurries after low temperature self-dissolving, and process after 10 minutes with 500 watts of ultrasonic waves (400 nanometer), the pH value to 8.0 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 1 hour at 20 DEG C.Zymolyte is crossed to 160 objects sieves, and filtered solution, through taking drying tower EAT as 170 DEG C, delivery temperature as 85 DEG C sprays and are dried, makes dry powder.
Embodiment 24
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 30 watts, and 250 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 30 minutes ultraviolet irradiations of described broken things.To the water of 2 times of volumes in the slurries after low temperature self-dissolving, and process after 10 minutes with 800 watts of ultrasonic waves (200 nanometer), the pH value to 7.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 1 × 10 in described homogenate
6the low-temperature protease of U/kg substrate enzymolysis 4 hours at 10 DEG C.Zymolyte is crossed to 200 objects sieves, and filtered solution, through taking drying tower EAT as 180 DEG C, delivery temperature as 75 DEG C sprays and are dried, makes dry powder.
Embodiment 25
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 30 watts, and 250 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 30 minutes ultraviolet irradiations of described broken things.To the water of 3 times of volumes in the slurries after low temperature self-dissolving, and process after 15 minutes with 800 watts of ultrasonic waves (300 nanometer), the pH value to 8.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 4 hours at 20 DEG C.Zymolyte is crossed to 200 objects sieves, and filtered solution, through taking drying tower EAT as 180 DEG C, delivery temperature as 95 DEG C sprays and are dried, makes dry powder.
Embodiment 26
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 40 watts, and 250 nanometer ultraviolet rays that distance is 0.5 meter descend self-dissolving 1 hour 0 DEG C (fishing for area surroundings temperature) after irradiating 10 minutes ultraviolet irradiations of described broken things.To the water of 1 times of volume in the slurries after low temperature self-dissolving, and process after 5 minutes with 300 watts of ultrasonic waves (400 nanometer), the pH value to 7.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 1 hour at 10 DEG C.Zymolyte is crossed to 120 objects sieves, and filtered solution, through taking drying tower EAT as 150 DEG C, delivery temperature as 75 DEG C sprays and are dried, makes dry powder.
Embodiment 27
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 40 watts, and 300 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 3 hours 2 DEG C (fishing for area surroundings temperature) after irradiating 10 minutes ultraviolet irradiations of described broken things.To the water of 1 times of volume in the slurries after low temperature self-dissolving, and process after 10 minutes with 500 watts of ultrasonic waves (200 nanometer), the pH value to 8.0 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 1 × 10 in described homogenate
6the low-temperature protease of U/kg substrate enzymolysis 3 hours at 10 DEG C.Zymolyte is crossed to 160 objects sieves, and filtered solution, through taking drying tower EAT as 150 DEG C, delivery temperature as 95 DEG C sprays and are dried, makes dry powder.
Embodiment 28
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 40 watts, and 300 nanometer ultraviolet rays that distance is 0.75 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 20 minutes ultraviolet irradiations of described broken things.To the water of 3 times of volumes in the slurries after low temperature self-dissolving, and process after 10 minutes with 500 watts of ultrasonic waves (300 nanometer), the pH value to 8.0 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 1 hour at 20 DEG C.Zymolyte is crossed to 160 objects sieves, and filtered solution, through taking drying tower EAT as 170 DEG C, delivery temperature as 85 DEG C sprays and are dried, makes dry powder.
Embodiment 29
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 40 watts, and 300 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 30 minutes ultraviolet irradiations of described broken things.To the water of 2 times of volumes in the slurries after low temperature self-dissolving, and process after 10 minutes with 800 watts of ultrasonic waves (400 nanometer), the pH value to 7.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 1 × 10 in described homogenate
6the low-temperature protease of U/kg substrate enzymolysis 4 hours at 10 DEG C.Zymolyte is crossed to 200 objects sieves, and filtered solution, through taking drying tower EAT as 180 DEG C, delivery temperature as 75 DEG C sprays and are dried, makes dry powder.
Embodiment 30
The fresh krill of fishing for is drained to rear squeezing broken, broken thing is through 40 watts, and 250 nanometer ultraviolet rays that distance is 1 meter descend self-dissolving 5 hours 4 DEG C (fishing for area surroundings temperature) after irradiating 30 minutes ultraviolet irradiations of described broken things.To the water of 3 times of volumes in the slurries after low temperature self-dissolving, and process after 15 minutes with 800 watts of ultrasonic waves (350 nanometer), the pH value to 8.5 that regulates described broken thing suspension with the HCl of 1M or NaOH adds 9 × 10 in described homogenate
5the low-temperature protease of U/kg substrate enzymolysis 4 hours at 20 DEG C.Zymolyte is crossed to 200 objects sieves, and filtered solution, through taking drying tower EAT as 180 DEG C, delivery temperature as 95 DEG C sprays and are dried, makes dry powder.
The above; it is only preferably detailed description of the invention of the present invention; but protection scope of the present invention is not limited to this; any be familiar with those skilled in the art the present invention disclose technical scope in; be equal to replacement or changed according to technical scheme of the present invention and inventive concept thereof, within all should being encompassed in protection scope of the present invention.
Claims (5)
1. a fast preparation method on the ship of krill concentrate and powder, comprises the steps:
S1, raw material processing: the fresh krill of fishing for is drained to rear squeezing broken, obtain broken thing;
S2, low temperature self-dissolving: use the ultraviolet ray of 20～40 watts of power, wavelength 200～300 nanometers to irradiate described broken thing 10～30 minutes apart from 0.5～1 meter; Self-dissolving 1～5 hour at 0～4 DEG C afterwards;
S3, ultrasonic assisted cryogenic external source enzymolysis: in the described broken thing obtaining to S2 step, add the water of 1～3 times of volume, process 5～15 minutes with the ultrasonic waves of 300～800 watts, regulate pH value to 7.5～8.5, add 9～10 × 10
5the low-temperature protease of U/kg substrate, at 10～20 DEG C, enzymolysis obtains zymolyte for 1～4 hour;
S4, zymolyte is crossed to 120～200 objects sieve to obtain filtered solution; Described filtered solution or after cryogenic vacuum is concentrated solid content account for 40～70% krill concentrate, or after spray-dried krill dry powder.
2. fast preparation method on the ship of krill concentrate and powder according to claim 1, is characterized in that, low-temperature protease described in step S3 is Novi's letter wave power low-temperature protease.
3. fast preparation method on the ship of krill concentrate and powder according to claim 2, is characterized in that, in step S3, regulating pH value to select concentration is HCl or the NaOH of 1～6M.
4. according to fast preparation method on the ship of the arbitrary described krill concentrate of claim 1～3 and powder, it is characterized in that, in step S4, carry out cryogenic vacuum with 20～25 DEG C of thickening temperatures, vacuum 75～90kPa and concentrate, make concentrate.
5. according to fast preparation method on the ship of the arbitrary described krill concentrate of claim 1～3 and powder, it is characterized in that, in step S4, taking drying tower EAT as 150～180 DEG C, delivery temperature as 75～95 DEG C are sprayed dryly, make dry powder.
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| CN1318324A (en) * | 2001-05-18 | 2001-10-24 | 湛江海洋大学 | Fast shrimp tissue self-dissolving method |
| CN101892280A (en) * | 2010-06-29 | 2010-11-24 | 中国水产科学研究院南海水产研究所 | Preparation method of pinctada fucata martensii meat anti-oxidizing oligopeptides |
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| CN1318324A (en) * | 2001-05-18 | 2001-10-24 | 湛江海洋大学 | Fast shrimp tissue self-dissolving method |
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