CN103283930B - Method for comprehensively utilizing euphausia superba shells - Google Patents
Method for comprehensively utilizing euphausia superba shells Download PDFInfo
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- CN103283930B CN103283930B CN201310198804.2A CN201310198804A CN103283930B CN 103283930 B CN103283930 B CN 103283930B CN 201310198804 A CN201310198804 A CN 201310198804A CN 103283930 B CN103283930 B CN 103283930B
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Abstract
The invention discloses a method for comprehensively utilizing euphausia superba shells. The method is a comprehensive utilizing method, wherein the shells are hydrolyzed by means of endogenous proteinases and exogenous alkaline proteinases of euphausia superba with high activity based on euphausia superba shells as raw materials to recover proteins and astaxanthin, while residues are used for producing chitin. The shells are hydrolyzed by means of endogenous proteinases and exogenous alkaline proteinases, so that the protein recovery rate is effectively increased. Enzymatic hydrolysates are filtered and centrifugalized, and the filtrate is concentrated to obtain protein concentrate or sprayed and dried to obtain soluble protein powder. The protein content in the protein powder is 75-85%. Precipitates are freezed and dried to obtain the protein powder rich in astaxanthin, wherein the protein content is 50-60% while the astaxanthin content is 600-900mg/kg. The addition amount of exogenous enzymes is reduced by 40-50% by replacing part of exogenous enzymes by enzymolysis of endogenous proteinases in a way of combining the characteristic that the endogenous proteinases of the euphausia superba are high in activity, so that the cost is greatly lowered.
Description
Technical field
The invention provides a kind of method with comprehensive utilization krill shell, belong to technical field of food biotechnology.
Background technology
Krill is subordinate to arthropods, Crustachia, Euphausiacea, is single living resources of planting of quantity maximum on the earth.The krill that lives in marine site, the South Pole has 7～8 kinds, can be for the very big krill of krill general guideline (Euphausia superb) of exploitation.Krill is unusual in many aspects, is one of multicellular animals that resource is the abundantest in the world, is also the enzyme producer who finds that so far decomposing protein is the strongest, is one of maximum animal assembling of marine organisms on the current earth.Krill is as single one of living resources of planting maximum on the earth, and annual quantity of the catch is equivalent to the summation of the annual fish in the current whole world and shell-fish catch, and being considered to maximum on our this celestial body is also last animal protein storehouse.Krill resource is as the strategic affluent resources of one, by the focus of exploitation that is following various countries.China, must quicken one's step still in the starting stage to the research and development of krill.
The processing of shrimp, as important ocean industry, can produce a large amount of discarded objects in process.Along with the increase of world population and the minimizing of resource, in the world the recycling of resource is very paid attention to.Discarded object after processing mainly comprises shrimp head and shrimp shell, and wherein contained shrimp sauce, astaxanthin, chitin and protein can be realized by reprocessing the comprehensive utilization of processing waste.At present both at home and abroad except leftover bits and pieces being directly used as to the processing that is mainly used in chitin feed.But the processing of current domestic chitin is mainly that soda acid consumption is large with chemical method, and protein is not recycled conventionally, causes the wasting of resources, and easily causes serious environmental pollution; Because the violent chemical reaction of high temperature of strong acid and strong base makes, chitin product quality is not high, fluctuation is also larger in addition.Both at home and abroad, be mainly to adopt alkaline process to reclaim protein, protein yield is low, and is damaged, and is of low nutritive value, and can only serve as common or low-grade feed; In recent years, in succession report that employing biological enzyme reclaims protein both at home and abroad, adopted biological enzyme to reclaim proteins react mild condition, little to the quality influence of protein and chitin, production process safety, environmental pollution is few.
The digestive system uniqueness of krill, has the enzyme system of powerful can degrade range protein and polysaccharide in its body.In krillase, due to its excision enzyme and restriction endonuclease ratio suitable, the range protein of can fast, fully degrading.In addition, a remarkable characteristic of krill endogenous proteinase be 0 ° still can protein hydrolysate below C, though this is unfavorable for the cold storage of krill, the processing of krill product is had to value.Therefore can utilize krill endogenous proteinase to its leftover protein matter enzymolysis, reclaim the albumen in leftover bits and pieces.
As mentioned above, the comprehensive utilization of research krill processing waste, utilizes enzyme process to replace the de-albumen of alkaline process, has improved albumen yield and has improved protein characteristic, has greatly reduced the soda acid consumption in follow-up chitin process, has reduced the pollution to environment.Utilize krill patio protease Substitute For Partial exogenous enzymes, greatly reduce production cost, for the comprehensive utilization that solves the discarded object after krill processing provides outlet and practicable method, there is significant society and environmental benefit simultaneously, will there is wide popularizing application prospect.
Summary of the invention
The object of this part is to summarize some aspects of embodiments of the invention and briefly introduces some preferred embodiments.In this part and the application's specification digest and denomination of invention, may do a little simplification or omit to avoid the making object of this part, specification digest and denomination of invention fuzzy, and this simplification or omit and can not be used for limiting the scope of the invention.
The problem existing in method in view of above-mentioned and/or existing comprehensive utilization krill shell, has proposed the present invention.
Therefore, object of the present invention is to propose a kind of method that fully utilizes krill shell, taking the fresh-keeping krill shell of cold storage technology as raw material, by endogenous proteinase and alkali protease enzymolysis successively, reclaim protein, its residue is for the production of the method for comprehensive utilization of chitin.
For solving the problems of the technologies described above, the invention provides following technical scheme: a kind of method that fully utilizes krill shell, taking krill shell as raw material, after enzyme hydrolysis, filtration, centrifugal treating, centrifuged supernatant is dry through Vacuum Concentration, spraying, make soluble protein powder, centrifugation obtains being rich in the albumen powder of astaxanthin through freeze drying; Wherein, described enzyme hydrolysis comprises endogenous enzymes hydrolysis and exogenous enzymes hydrolysis, and it is to add water than for 1:4～6 according to shrimp shell and water quality in krill shell that described endogenous enzymes is hydrolyzed, and utilizes self endogenous enzymes lucifuge stirring reaction 1h～2h at 50 ° of C～60 DEG C; Described exogenous enzymes hydrolysis is after endogenous enzymes hydrolysis finishes, and regulates pH8.0～8.5, adds Alcalase2.4L alkali protease, and enzyme concentration is 1500U/g～3000U/g protein, lucifuge stirring reaction 1.5h～2.5h at 50 DEG C～60 DEG C; Described filtration is that exogenous enzymes hydrolysis finishes rear enzymolysis liquid through 60 order～100 mesh filter screens filtrations, obtains filtrate and filter residue; Described centrifugal be filtrate through the centrifugal 15min of 3000r/min～5000r/min, supernatant is separated with sediment; Described Vacuum Concentration is that centrifuged supernatant Vacuum Concentration is for subsequent use to soluble solid content 30%～35%; Described spraying is dry is that the supernatant after concentrated spray and is dried, 170 ° of C～180 DEG C of EAT, and 80 DEG C of leaving air temps, obtain protein content and are 75%～85% albumen powder; Described freeze drying is by centrifugal sediment freeze drying, obtains being rich in the albumen powder of astaxanthin, and wherein protein content is 50%～60%, and content astaxanthin is 600～900mg/kg.
As a kind of preferred version of the method for comprehensive utilization krill shell of the present invention, wherein: described raw material adopts freezing krill, through thawing, on flesh separator, adopt after meat three times, remaining residue is krill shell, gross protein value 10% left and right.
The invention provides a kind of method that fully utilizes krill shell, by the effect successively of krill endogenous proteinase and alkali protease, protein in high efficiente callback krill shell, and make chitin, protein and astaxanthin be recycled utilization simultaneously, improve the utilization rate of resource, improve value-added content of product, thereby in enzymolysis process, greatly reduced the consumption minimizing cost of alkali protease simultaneously, increased economic benefit.Utilize enzyme digestion reaction, mild condition, the protein quality of production is high, obtains being rich in the protein of astaxanthin, can be used as flavouring or extraordinary fish meal.Krill individuality is little, has reduced crushing process, and the chitin quality that obtains is high, steady quality, has promoted the technological transformation of chitin industry and the raising of technical merit.To the comprehensive utilization of krill processing fent, can not only make full use of resource, reduce environmental pollution, can also be used for development of new food, create huge economic benefit, drive whole industry to advance, extend industrial chain, realizes economic sustainable development.
Brief description of the drawings
In embodiment of Fig. 1, fully utilize krill casing tech FB(flow block).
Detailed description of the invention
Below in conjunction with specific embodiment, the method for the invention is elaborated.
Describe embodiments of the invention below in detail, the example of described embodiment is shown in the drawings, and wherein same or similar label represents same or similar element or has the element of identical or similar functions from start to finish.Be exemplary below by the embodiment being described with reference to the drawings, only for explaining the present invention, and can not be interpreted as limitation of the present invention.
Embodiment 1
Raw material adopts the krill shell of cold storage, and shrimp shell is added to water by shrimp shell/water quality than 1:4, is heated to 50 DEG C, insulation, lucifuge stirring reaction 2h.Regulate pH8.0, add alkali protease to carry out enzymolysis, consumption is 3000U/g protein by enzyme work, lucifuge stirring reaction 1.5h, and reaction finishes rear use 60 mesh filter screens and filters.Filtrate, in the centrifugal 15min of 4000rpm, separates supernatant with sediment.Supernatant Vacuum Concentration, is concentrated to soluble solid and is 35% and spray dryly, obtains albumen powder, and protein content is 85%.Centrifugal sediment freeze drying, obtains being rich in the albumen powder of astaxanthin, and wherein protein content is 50%, and content astaxanthin is 900mg/kg.After filtering, remaining residue is for the preparation of chitin.
Embodiment 2
Raw material adopts the krill shell of cold storage, and krill shell is added to water by krill shell/water quality than 1:6, is heated to 60 DEG C, insulation, lucifuge stirring reaction 1.5h.Regulate pH8.0, add alkali protease to carry out enzymolysis, consumption is 1500U/g protein by enzyme work, lucifuge stirring reaction 2.5h, reaction finishes rear use 60 mesh filter screens filters, and by enzymolysis liquid centrifugal 15min under the condition of 5000rpm, supernatant is separated with sediment.Supernatant Vacuum Concentration, is concentrated to soluble solid and is 32% and spray dryly, obtains albumen powder, and protein content is 82%.Centrifugal sediment freeze drying, obtains being rich in the albumen powder of astaxanthin, and wherein protein content is 55%, and content astaxanthin is 750mg/kg.After filtering, remaining residue is for the preparation of chitin.
Embodiment 3
Raw material adopts the krill processing fent of cold storage, and leftover bits and pieces is added to water by leftover bits and pieces/water quality than 1:5, is heated to 55 DEG C, insulation, lucifuge stirring reaction 1h.Regulate pH8.5, add alkali protease to carry out enzymolysis, consumption is 2000U/g protein by enzyme work, lucifuge stirring reaction 2h, and reaction finishes rear use 60 mesh filter screens and filters.Filtrate is centrifugal 15min under the condition of 3000rpm, and supernatant is separated with sediment.Supernatant Vacuum Concentration, is concentrated to soluble solid and is 30% and spray dryly, obtains albumen powder, and protein content is 75%.Centrifugal sediment freeze drying, obtains being rich in the albumen powder of astaxanthin, and wherein protein content is 60%, and content astaxanthin is 600mg/kg.Other operate with embodiment 1.After filtering, remaining residue is for the preparation of chitin.
Comprehensive embodiment 1～3, the present invention is by through the fresh-keeping krill shell of freezing technology, add a certain proportion of water, utilize after self intrinsic protein enzyme hydrolysis, add hydrolysis by novo, filtered solution is through albumen powder concentrated, spraying obtains being rich in astaxanthin after being dried again, and the residue after recovery protein is for the production of chitin, sewage reaches emission request after treatment, and described method comprises the steps:
(1) raw material: process raw material through the fresh-keeping krill processing fent of freezing technology;
(2) add water: add water to krill processing fent, the mass ratio of shrimp processing fent and water is 1:4～6;
(3) protease hydrolytic: utilize the efficient protease hydrolytic system of krill self, at 50～60 DEG C, after lucifuge stirring reaction 1～2h, regulate pH8.0～8.5, add alkali protease, enzyme concentration is 1500～3000U/g protein, lucifuge stirring reaction 1.5～2.5h at 50～60 DEG C;
(4) filter: enzymolysis liquid filters through 60～100 mesh filter screens;
(5) centrifugal: the centrifugal 15min of 3000～5000rpm, separates supernatant with sediment;
(6) spraying is dry: filtered solution adopts Vacuum Concentration, is concentrated to soluble solid 30%～35%, spray dry, and the albumen powder that protein content is 75%～85%;
(7) freeze drying: by sediment freeze drying, obtain being rich in the albumen powder of astaxanthin, protein content is 50%～60%, and content astaxanthin is 600～900mg/kg;
(8) residue treatment: residue obtained for the production of chitin after filtering.
The present invention utilizes endogenous enzymes, the comprehensive prawn shell of exogenous enzymes to be hydrolyzed, effectively increase protein recovery, enzymolysis product is centrifugal after filtering, the concentrated protein concentrate obtaining of filtered solution warp or the more spray-dried soluble protein powder that obtains, protein content 75～85% in albumen powder.Sediment obtains being rich in the albumen powder of astaxanthin through freeze drying, wherein protein content is 50%～60%, and content astaxanthin is 600～900mg/kg.In conjunction with the high feature of krill self intrinsic protein enzyme activity, utilize the enzymolysis Substitute For Partial exogenous enzymes of endogenous proteinase, make exogenous enzymes addition reduce 40～50%, greatly reduce cost.
It should be noted that, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement technical scheme of the present invention, and not departing from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of claim scope of the present invention.
Claims (2)
1. one kind fully utilizes the method for krill shell, it is characterized in that: taking krill shell as raw material, after enzyme hydrolysis, filtration, centrifugal treating, centrifuged supernatant is dry through Vacuum Concentration, spraying, make soluble protein powder, centrifugation obtains being rich in the albumen powder of astaxanthin through freeze drying; Wherein,
Described enzyme hydrolysis comprises endogenous enzymes hydrolysis and exogenous enzymes hydrolysis, and it is to add water than for 1:4～6 according to shrimp shell and water quality in krill shell that described endogenous enzymes is hydrolyzed, and utilizes self endogenous enzymes lucifuge stirring reaction 1h～2h at 50 ° of C～60 DEG C; Described exogenous enzymes hydrolysis is after endogenous enzymes hydrolysis finishes, and regulates pH8.0～8.5, adds Alcalase2.4L alkali protease, and enzyme concentration is 1500U/g～3000U/g protein, lucifuge stirring reaction 1.5h～2.5h at 50 DEG C～60 DEG C;
Described filtration is that exogenous enzymes hydrolysis finishes rear enzymolysis liquid through 60 order～100 mesh filter screens filtrations, obtains filtrate and filter residue;
Described centrifugal be filtrate through the centrifugal 15min of 3000r/min～5000r/min, supernatant is separated with sediment;
Described Vacuum Concentration is that centrifuged supernatant Vacuum Concentration is for subsequent use to soluble solid content 30%～35%;
Described spraying is dry is that the supernatant after concentrated spray and is dried, 170 ° of C～180 DEG C of EAT, and 80 DEG C of leaving air temps, obtain protein content and are 75%～85% albumen powder;
Described freeze drying is by centrifugal sediment freeze drying, obtains being rich in the albumen powder of astaxanthin, and protein content is 50%～60%, and content astaxanthin is 600～900mg/kg.
2. the method for comprehensive utilization krill shell according to claim 1, is characterized in that: described raw material adopts freezing krill, through thawing, on flesh separator, adopt after meat three times, remaining residue is krill shell, gross protein value 10% left and right, and cold storage is for subsequent use.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105542936A (en) * | 2015-12-16 | 2016-05-04 | 江南大学 | Euphausia superba oil extraction method |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105420326A (en) * | 2015-12-29 | 2016-03-23 | 广西钦州市绿源天然食品加工有限公司 | Method for extracting proteins and astaxanthin from shrimp shells through papain hydrolysis |
| CN105707470A (en) * | 2016-03-02 | 2016-06-29 | 湛江市博泰生物化工科技实业有限公司 | Paste amino acid product prepared from euphausia superba or processing leftovers thereof and preparation method of paste amino acid product |
| CN106798073A (en) * | 2017-01-12 | 2017-06-06 | 广东泰宝医疗器械技术研究院有限公司 | One kind method for extracting proteins from krill |
| CN108130353A (en) * | 2017-11-22 | 2018-06-08 | 浙江海洋大学 | A kind of method that oligopeptides powder is prepared using krill shell |
| US11421045B2 (en) * | 2018-06-12 | 2022-08-23 | Rimfrost Technologies As | Krill-derived chitin products and methods of making same |
| CN110463930A (en) * | 2019-09-05 | 2019-11-19 | 福建坤兴海洋股份有限公司 | One seed shrimp is sliding and its shrimp slides preparation method |
| CN110720623A (en) * | 2019-10-14 | 2020-01-24 | 浙江海洋大学 | Method for preparing seasoning by using euphausia superba processing by-product |
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| JP2004041105A (en) * | 2002-07-12 | 2004-02-12 | Nippon Suisan Kaisha Ltd | Feed for fish and shellfish |
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| CN102559368A (en) * | 2010-12-14 | 2012-07-11 | 大连工业大学 | Preparation method of antarctic krill phospholipid |
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