CN105707470A - Paste amino acid product prepared from euphausia superba or processing leftovers thereof and preparation method of paste amino acid product - Google Patents
Paste amino acid product prepared from euphausia superba or processing leftovers thereof and preparation method of paste amino acid product Download PDFInfo
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- CN105707470A CN105707470A CN201610115393.XA CN201610115393A CN105707470A CN 105707470 A CN105707470 A CN 105707470A CN 201610115393 A CN201610115393 A CN 201610115393A CN 105707470 A CN105707470 A CN 105707470A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- General Life Sciences & Earth Sciences (AREA)
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Abstract
The invention discloses a paste amino acid product prepared from euphausia superba or processing leftovers thereof and a preparation method of the paste amino acid product. The preparation method comprises the steps that euphausia superba or processing leftovers thereof is/are taken as raw materials, after shells and meat are separated, enzymolysis is performed on meat slurry to form amino acid liquid, the euphausia superba shells are hydrolyzed with acid to form glucosamine liquid, the amino acid liquid and the glucosamine liquid are mixed, evaporated and concentrated until the water content is 30%-45%, and then the paste product rich in glucosamine, various amino acids, astacin and shrimp phospholipids is obtained and can serve as an efficient feed additive and an agricultural efficient soil conditioner. The problems that in the existing euphausia superba fishing process, the transportation and preservation cost and the fluorine content are high are solved; the preparation method has the advantages that operation is easy, the processing capacity of high, zero discharge is achieved in the processing process, the product performance is superior, the additional value is high, and a novel way is exploited for the field of euphausia superba downstream processing and applying.
Description
Technical field
The present invention relates to field of fodder, specifically, relate to a kind of paste amino acid products prepared by Antarctic krill or its processing fent and preparation method thereof.
Background technology
Antarctic krill is one of biological variety that on the earth, stock number is maximum.According to statistics, the Biomass of Antarctic krill resource is guarded estimation and be there are about 6.5 ~ 1,000,000,000 tons.Antarctic krill is the species that in the biology that the mankind are found up to now, protein content is the highest, and protein content is high and excellent, and the protein content of krill meat is 17.65%, and dry krill is then up to 65%, is two to three times of other animal foods.The amino acid classes that Antarctic krill contains is complete, and the especially content of lysine is the abundantest, accounts for the 9.73% of protein content.Rich in various aminoacid, bioactive peptide, organic acid etc. in Antarctic krill enzymolysis solution.
At present, the exploitation about Antarctic krill essentially consist in the edible extraction aspect with antarctic krill oil of human body, but there is the problem that Oil repellent is too high in Antarctic krill, and human body is edible can exist healthy hidden danger.To this end, Chinese Marine University and Dalian University of Technology propose relevant defluorination method, and apply for that respectively at 2010 and 2011 acid-base precipitation method prepares the patent of Antarctic krill low fluorine albumen.Meanwhile, Liaoning Dalian Ocean Fishery Group Corporations has also applied for the patent (CN 102559825 A) of calcium oxide defluorinate.Although defluorinate processes, and the Oil repellent of Antarctic krill albumen can be made a certain degree of reduction, but with the addition of soda acid or additive, there is hygienic issues, even if there is also Halothane gene hidden danger after defluorinate, so edibility is the lowest.Visible, Antarctic krill is directly used in and prepares human body food, it is worth and the market space is the most little, and this limits the development of China's Antarctic krill fishery significantly.Therefore exploitation Antarctic krill is new high added value purposes and novel green process technology are necessary and significant.
Agricultural is the industry that China will give priority to future, but China's current soil major part exist barren, pollute, harden and acidifying etc. problem, this is the huge problem that China's agricultural development faces.China to be realized agricultural is healthy, sustainable development, and exploitation efficient, novel soil conditioner is imperative.The soil conditioner quality that market occurs at present is uneven, generally stores the undesirable problem of effect.Through test, the shrimp peptide soil conditioner using krill and krill processing fent to prepare, there is in terms of soil improvement and crop alimentary extremely unique effect.Application test is 200 mu of experimental plots of Yunnan Zhong Yan group of Tian Hua limited company and patent application unit shared, Zhanyi, Yunnan great Po Ma street, testing site.It is reported, these 200 mu of model fields have 50 mu for total crop failure in continuous 3 years, the soil conditioner using Antarctic krill extremely leftover bits and pieces to prepare in 2015 is used, use 8 ~ 10kg for every mu, the tobacco leaf planting base reclamation result in this experimental plot is the best, and quality of tobacco reaches international High Quality Tobacco level, high yield good harvest, yield of tobacco improves 100%, and quality of tobacco is largely increased simultaneously.Hong Yun Red River group high level, Research Institute of Chemical Industry president, Yuntianhua share general manager etc. lead all to site visit, the effect of " tobacco planting shrimp peptide chitin soil improvement and integrated control " model field is given high evaluation, and causes the media such as www.xinhuanet.com's Yunnan channel, People's Net's Yunnan channel, Yunnan Daily, Yunnan television station, Economic In Yunnan daily paper and fall over each other report and surging pay close attention to.So far, not yet there is disclosed research or the report preparing efficient soil conditioner about Antarctic krill or its processing fent.
The Chinese invention patent of Application No. 2014103397234 discloses " a kind of method utilizing low value shrimp and shrimping beam trawl to prepare shrimp cream ", this patent uses low value shrimp and shrimping beam trawl and carries out Maillard reaction formation flavor substance as raw material by sugar and aminoacid, preparing food shrimp cream product, process need to use the complex technologys such as supercritical carbon dioxide extraction.All there is the biggest difference with patent of the present invention in shrimp raw material type, technical method and the product purpose that this patent uses.Present invention is generally directed to Antarctic krill, process route uses the piecewise processing technique of separating shell and meat.Acidolysis is carried out with acid prawn shell, Macrobrachium nipponensis uses enzymolysis, merge acid hydrolysis solution and enzymolysis solution and be concentrated to give rich in glucosamine, various aminoacid, 3,4,3',4'-tetraketo-.beta.-carotene and the creamy product of shrimp phospholipid, product is rich in aminoacid, 3,4,3',4'-tetraketo-.beta.-carotene, phosphoric acid class and aminoglucose sugar salt, for special efficacy feed raw material or agricultural use.
To sum up, the present invention has good novelty and feasibility, utilizes process technology of the present invention, and by friendly process, Antarctic krill and processing fent thereof are prepared as paste amino acid products, is to have huge value space for agricultural and feedstuff industry.
Summary of the invention
It is an object of the invention to transport and the preservation cost height overcoming current Antarctic krill to fish for process, and there is the thorny problems such as healthy hidden danger to edible greatly in Antarctic krill fluorinated volume, it is provided that a kind of paste amino acid products prepared by Antarctic krill or its processing fent.
To achieve these goals, the present invention adopts the following technical scheme that
A kind of paste amino acid products prepared by Antarctic krill or its processing fent, makes by the following method:
(1) fresh Antarctic krill or its processing fent are carried out separating shell and meat process:
(2) Crusta Penaeus seu Panulirus acid is hydrolyzed formation glucosamine salt, and described acid is hydrochloric acid, nitric acid, sulphuric acid, phosphoric acid or acetic acid；The consumption of described acid is the 5 ~ 30% of Crusta Penaeus seu Panulirus quality；Acid hydrolysis solution uses adjusting PH with base at 7-10, and described alkali is NaOH, KOH, GaO, MgO, Na2CO3、K2CO3, Ga (OH)2Or Mg (OH)2In one or more mixing；
(3) the meat serosity separated carries out enzymolysis processing, described enzyme is one or more mixing in cellulolytic enzyme, pepsin, trypsin, cathepsin, papain, subtilisin, serine protease, thiol protease, metalloproteases and aspartic protease, hydrolysis temperature 40 ~ 60 DEG C, the quality of enzyme is the 0.04 ~ 0.1% of substrate quality, and enzymolysis time is 0.5 ~ 3 hour；It is centrifuged processing to meat slurry enzymolysis solution, by the Protein Separation of non-for bulky grain thorough enzymolysis and return enzymolysis workshop section；
(4) meat slurry enzymolysis solution and Crusta Penaeus seu Panulirus acid hydrolysis solution merge and are mixed, then to carry out removing water to moisture content be 30 ~ 45%.
As preferably, in above-mentioned paste amino acid products, the method that described separating shell and meat processes is homogenate screening, extruding separates or mechanical stripping separates.
As preferably, in above-mentioned paste amino acid products, step (2) described acid is hydrochloric acid or sulphuric acid, and the consumption of described acid is the 10-20% of Crusta Penaeus seu Panulirus quality.
As preferably, in above-mentioned paste amino acid products, the acid hydrolysis solution adjusting PH with base of step (2) is at 8-9.Described alkali most preferably GaO or MgO.
As preferably, in above-mentioned paste amino acid products, enzyme used by step (3) is cellulase, trypsin or papain.
As preferably, in above-mentioned paste amino acid products, step (3) described centrifugal treating is that plate-and-frame filtration separation, centrifugal filtration separation or centrifugal sedimentation separate.
As preferably, in above-mentioned paste amino acid products, step (4) described water-eliminating method removes water for conventional evaporate, be evaporated under reduced pressure, reduce pressure forced-circulation evaporation or centrifugal sedimentation.
Compared with prior art, there is advantages that
(1) Antarctic krill and processing fent thereof are prepared as paste amino acid products by the present invention, are substantially reduced Antarctic krill and fish for preservation and the cost of transportation problem of process.
(2) the paste aminoacid that the present invention obtains can be used for high-efficiency feed additive and agricultural high-effiency soil conditioner.Avoid the Antarctic krill fluorinated volume healthy hidden danger drawback of high existence edible to human body, develop new higher value application approach for Antarctic krill.As soil conditioner, its added value and function exceed human body and eat.Utilizing this modifying agent, every mu of ground to use 5-15kg, crop yield at least can improve 30%, even several times.
(3) preparation method of the present invention is simple to operate, and process is green, and course of processing zero-emission, properties of product are superior.The rapid enzymolysis technique used, just can be hydrolyzed krill albumen at 40 minutes the soonest.
Detailed description of the invention
Embodiment 1: paste amino acid products preparation process is as follows:
(1) fresh Antarctic krill employing homogenate method for sieving is carried out separating shell and meat process:
(2) Crusta Penaeus seu Panulirus hydrochloric acid is hydrolyzed formation glucosamine salt；Hydrochloric acid consumption is the 10% of Crusta Penaeus seu Panulirus quality；Acid hydrolysis solution GaO adjusts pH 8.
(3) the meat serosity separated uses cellulolytic enzyme to carry out enzymolysis processing；Hydrolysis temperature is 40 DEG C, and cellulolytic enzyme quality is the 0.04% of substrate quality, and enzymolysis time is 0.5 hour；Meat slurry enzymolysis solution is carried out plate-and-frame filtration separation, the albumen of non-for bulky grain thorough enzymolysis is returned enzymolysis workshop section；
(4) meat slurry enzymolysis solution and Crusta Penaeus seu Panulirus acid hydrolysis solution merge and are mixed, then carry out reduction vaporization to remove water to moisture content be 30 ~ 45%, obtains rich in glucosamine, various aminoacid, 3,4,3',4'-tetraketo-.beta.-carotene and the creamy product of shrimp phospholipid.
Gained paste amino acid products outward appearance is brown, and abnormal smells from the patient is corrupt abnormal smells from the patient, and glucosamine hydrochloride content is more than 4%, and various total amino acid content are more than 45%, and 3,4,3',4'-tetraketo-.beta.-carotene content is more than 200ppm, and shrimp content of phospholipid is more than 0.2%.Products obtained therefrom, typically not as feed additive, compares and is suitable as agromelioration agent and heavy metal-polluted soil renovation agent, and consumption is every mu and uses 5-15kg, excellent.
Embodiment 2: paste amino acid products preparation process is as follows:
(1) the processing fent employing extruding separation method of fresh Antarctic krill is carried out separating shell and meat process:
(2) Crusta Penaeus seu Panulirus sulphuric acid is hydrolyzed formation glucosamine salt；Acid amount used is the 20% of Crusta Penaeus seu Panulirus quality；Acid hydrolysis solution MgO adjusts pH 9；
(3) the meat serosity separated uses trypsin to carry out enzymolysis processing, hydrolysis temperature 60 DEG C, and the quality of enzyme is the 0.1% of substrate quality, and enzymolysis time is 3 hours；It is centrifuged filtering to meat slurry enzymolysis solution and separates, by the Protein Separation of non-for bulky grain thorough enzymolysis and return enzymolysis workshop section；
(4) meat slurry enzymolysis solution and Crusta Penaeus seu Panulirus acid hydrolysis solution merge and are mixed, then be centrifuged sedimentation to remove water to moisture content be 30 ~ 45%, obtains rich in glucosamine, various aminoacid, 3,4,3',4'-tetraketo-.beta.-carotene and the creamy product of shrimp phospholipid.
Gained paste amino acid products color is yellow, abnormal smells from the patient is dense fragranced, glucosamine sulphate content is more than 5%, various total amino acid content are more than 45%, 3,4,3',4'-tetraketo-.beta.-carotene content is more than 200ppm, and shrimp content of phospholipid is more than 0.2%, and microorganism and mycete index reach feed grade, can be as high effective feed protein source, feedstuff per ton can add 30-50kg；Can also be as agricultural high-effiency soil conditioner or heavy metal-polluted soil renovation agent, every mu of amount of application 5-15kg, excellent.
Embodiment 3: paste amino acid products preparation process is as follows:
(1) method that fresh Antarctic krill uses mechanical stripping to separate with its processing fent is carried out separating shell and meat process:
(2) Crusta Penaeus seu Panulirus acetic acid is hydrolyzed formation glucosamine salt；Acid amount used is the 30% of Crusta Penaeus seu Panulirus quality；Acid hydrolysis solution adjusting PH with base is 10, and alkali used is NaOH and MgO mixing；
(3) the meat serosity separated carries out enzymolysis processing, and enzyme used is cathepsin and papain mixing, and hydrolysis temperature 50 DEG C, the quality of enzyme is the 0.08% of substrate quality, and enzymolysis time is 2 hours；It is centrifuged processing to meat slurry enzymolysis solution, by the Protein Separation at non-for bulky grain enzymolysis-end and return enzymolysis workshop section；
(4) meat slurry enzymolysis solution and Crusta Penaeus seu Panulirus acid hydrolysis solution merge and are mixed, then carry out reducing pressure that to remove water to moisture content be 30 ~ 45% in forced-circulation evaporation, obtain rich in glucosamine, various aminoacid, 3,4,3',4'-tetraketo-.beta.-carotene and the creamy product of shrimp phospholipid.
Gained paste amino acid products is light yellow, abnormal smells from the patient is fragrant, aminoglucose acetate content is lower than hydrochloric acid and sulfur process, being about 1%, various total amino acid content are more than 45%, and 3,4,3',4'-tetraketo-.beta.-carotene and content of phospholipid are more than hydrochloric acid and sulfur process, content respectively reaches 300ppm and more than 0.3%, microorganism and mycete index can arrive feed grade, can be as high effective feed protein source, and feedstuff per ton can add 30-50kg；Can also be as agricultural high-effiency soil conditioner or heavy metal-polluted soil renovation agent, every mu of amount of application 5-15kg, excellent.
Claims (8)
1. the paste amino acid products prepared by Antarctic krill or its processing fent, it is characterised in that make by the following method:
(1) fresh Antarctic krill or its processing fent are carried out separating shell and meat process:
(2) Crusta Penaeus seu Panulirus acid is hydrolyzed formation glucosamine salt, and described acid is hydrochloric acid, nitric acid, sulphuric acid, phosphoric acid or acetic acid；The consumption of described acid is the 5 ~ 30% of Crusta Penaeus seu Panulirus quality；Acid hydrolysis solution uses adjusting PH with base at 7-10, and described alkali is NaOH, KOH, GaO, MgO, Na2CO3、K2CO3, Ga (OH)2Or Mg (OH)2In one or more mixing；
(3) the meat serosity separated carries out enzymolysis processing, described enzyme is one or more mixing in cellulolytic enzyme, pepsin, trypsin, cathepsin, papain, subtilisin, serine protease, thiol protease, metalloproteases and aspartic protease, hydrolysis temperature 40 ~ 60 DEG C, the quality of enzyme is the 0.04 ~ 0.1% of substrate quality, and enzymolysis time is 0.5 ~ 3 hour；It is centrifuged processing to meat slurry enzymolysis solution, by the Protein Separation of non-for bulky grain thorough enzymolysis and return enzymolysis workshop section；
(4) meat slurry enzymolysis solution and Crusta Penaeus seu Panulirus acid hydrolysis solution merge and are mixed, then to carry out removing water to moisture content be 30 ~ 45%.
2. paste amino acid products as claimed in claim 1, it is characterised in that the method that described separating shell and meat processes is homogenate screening, extruding separates or mechanical stripping separates.
3. paste amino acid products as claimed in claim 1, it is characterised in that in step (2), described acid is hydrochloric acid or sulphuric acid, acid amount used is the 10-20% of Crusta Penaeus seu Panulirus quality.
4. paste amino acid products as claimed in claim 1, it is characterised in that in step (2), acid hydrolysis solution uses adjusting PH with base at 8-9.
5. paste amino acid products as claimed in claim 1, it is characterised in that described alkali is GaO or MgO.
6. paste amino acid products as claimed in claim 1, it is characterised in that in step (3), described enzyme is cellulase, trypsin or papain.
7. paste amino acid products as claimed in claim 1, it is characterised in that in step (3), described centrifugal treating is that plate-and-frame filtration separation, centrifugal filtration separation or centrifugal sedimentation separate.
8. paste amino acid products as claimed in claim 1, it is characterised in that in step (4), described water-eliminating method removes water for conventional evaporate, be evaporated under reduced pressure, reduce pressure forced-circulation evaporation or centrifugal sedimentation.
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Cited By (4)
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| CN112602851A (en) * | 2020-12-24 | 2021-04-06 | 日照职业技术学院 | Multistage utilization method of euphausia superba powder |
| CN114847403A (en) * | 2022-03-23 | 2022-08-05 | 大香生物科技(烟台)有限公司 | Low-value animal raw material pet feed flavor enhancer |
| CN115281320A (en) * | 2022-07-28 | 2022-11-04 | 佛山市海天(高明)调味食品有限公司 | Shrimp meat zymolyte and preparation method thereof, and compound seasoning |
| CN115418232A (en) * | 2022-09-30 | 2022-12-02 | 山东农大肥业科技股份有限公司 | Soil conditioner prepared from glucosamine hydrochloride waste liquid and preparation method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112602851A (en) * | 2020-12-24 | 2021-04-06 | 日照职业技术学院 | Multistage utilization method of euphausia superba powder |
| CN112602851B (en) * | 2020-12-24 | 2022-09-02 | 日照职业技术学院 | Multistage utilization method of euphausia superba powder |
| CN114847403A (en) * | 2022-03-23 | 2022-08-05 | 大香生物科技(烟台)有限公司 | Low-value animal raw material pet feed flavor enhancer |
| CN115281320A (en) * | 2022-07-28 | 2022-11-04 | 佛山市海天(高明)调味食品有限公司 | Shrimp meat zymolyte and preparation method thereof, and compound seasoning |
| CN115281320B (en) * | 2022-07-28 | 2023-09-26 | 佛山市海天(高明)调味食品有限公司 | Shrimp meat zymolyte, preparation method thereof and compound seasoning |
| CN115418232A (en) * | 2022-09-30 | 2022-12-02 | 山东农大肥业科技股份有限公司 | Soil conditioner prepared from glucosamine hydrochloride waste liquid and preparation method and application thereof |
| CN115418232B (en) * | 2022-09-30 | 2024-03-19 | 山东农大肥业科技股份有限公司 | Soil conditioner prepared from glucosamine hydrochloride waste liquid and preparation method and application thereof |
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