CN103130914A - Method for preparing chitin and composite protein powder by composite microbial fermentation of prawn leftovers - Google Patents
Method for preparing chitin and composite protein powder by composite microbial fermentation of prawn leftovers Download PDFInfo
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- CN103130914A CN103130914A CN2011102952852A CN201110295285A CN103130914A CN 103130914 A CN103130914 A CN 103130914A CN 2011102952852 A CN2011102952852 A CN 2011102952852A CN 201110295285 A CN201110295285 A CN 201110295285A CN 103130914 A CN103130914 A CN 103130914A
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Abstract
The invention relates to a method for preparing chitin and composite protein powder by composite microbial fermentation of prawn leftovers, which comprises the following steps: homogenizing prawn leftovers, adding composite microbe (lactobacillus bulgaricus and bacillus subtilis) according to a certain ratio, fermenting for 48 hours, separating the prawn shells from the fermentation liquid, drying and crushing the prawn shells to obtain chitin, adjusting the pH of the fermentation liquid, adding powdery soybean meal, inoculating the bacillus subtilis, continuing to perform solid fermentation for 48 hours, drying after fermentation to prepare composite protein powder. The product prepared in the invention contains both microbial mycoprotein and vegetable protein degradation products and animal protein, is rich in nutrition, and has functional activity; The greateat advantage is the green utilization of prawn leftovers, which discharges no pollutant, realizes clean production, and obtains the products of chitin and composite protein powder with high added value.
Description
Technical field
The invention belongs to disposal from fishery product processing higher value application technical field, be specifically related to a kind of method of utilizing compound microorganism ferments prawn tankage to prepare chitin and compound protein powder.
Background technology
Global prawn culturing output was more than 350 ten thousand tons in 2010, China is still the whole world main prawn producing country, 134.8 ten thousand tons of cultured outputs, and breed variety is take Environment of Litopenaeus vannamei Low as main, account for 73% of cultivation total amount, all the other breed variety tigar prawns, Chinese prawn and japonicus respectively account for 7%, 6% and 6%.The consumption of prawn has three approach, and the one, outlet is mainly the shrimp that decaptitates; The 2nd, bright shrimp is edible; The 3rd, the processing peeled shrimp is domestic.No matter which kind of approach, all can produce a large amount of shrimp heads, shrimp shell and shrimp tail waste, accounts for 20%～50% of whole shrimp.Contain the nutritive ingredients such as a large amount of chitins and protein, unsaturated fatty acids, astaxanthin, mineral substance in these wastes; chitin can deacetylatedly change chitosan having multi-functions into; but present most of waste adopts the acid-base method chitin extraction; nitrogenous waste liquid is discharged; part waste abandons as refuse; increase the weight of on the one hand the pollution of environment, caused on the other hand the huge waste of resource, restricted the development of prawn processing industry.Therefore how scientifically utilize to environmental protection prawn shrimp head, shrimp shell, improve economic value added, realize that zero disposal of pollutants becomes present China problem demanding prompt solution.
By retrieval, find following three pieces of publication documents relevant to present patent application: 1, utilize Lactobacterium acidophilum to extract the method (CN102119759A) of protein and chitin from shrimp head and shrimp shell, described Lactobacterium acidophilum LactobacillusacidophilusSW01 is by Chinese Typical Representative culture collection center (CCTCC) preservation, can extract simultaneously protein and chitin from shrimp head and shrimp shell by Lactobacterium acidophilum LactobacillusacidophilusSW01 of the present invention, the protein hydrolysate that obtains is rich in calcium lactate and other mineral substance.2, water making beating, sieving separation are pulverized, added to a kind of fodder additives and working method (CN101926409A) thereof of utilizing shrimp head and vegetable feed proteins mixed fermentation with the shrimp head through squeezing, tells the shrimp shell and obtain shrimp slurry raw material; Shrimp slurry and vegetable feed proteins are mixed in proportion, add the bacterium solid state fermentation, then drying, pulverize and make the fermented feed protein powder; Can be used as chitin, shrimp shell meal raw material after the shrimp shell drying of separating.3, a kind of crust waste that utilizes prepares chitin, the method of Pfansteihl calcium and aminoacids complex or peptide (CN101962666A), be specially the crust waste at lactobacillus rhamnosus (Lactobacillus? rhamnosus) CICC6003 and subtilis (Bacillus? subtilis) obtain Pfansteihl calcium in the CICC23579 seed liquor after fermentation, then obtain aminoacids complex or peptide and chitin after fermentation in aspergillus oryzae (Aspergillus oryzae) CICC41477 seed liquor, can realize the decalcification of the crust wastes such as shrimp crab, degreasing and deproteinated, and prepare the chitin of high-quality, Pfansteihl calcium, aminoacids complex (peptide) series product, realize protein, the comprehensive utilization of Calcium Carbonate resource.
By comparison, the technical scheme of above-mentioned publication document all with present patent application have essential different, and exist preparation process consume energy excessive, pollutant emission or the undesirable deficiency of ferment effect arranged.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art part, a kind of method of utilizing compound microorganism ferments prawn tankage to prepare chitin and compound protein powder is provided.
The objective of the invention is to be achieved through the following technical solutions:
A kind of method of utilizing compound microorganism ferments prawn tankage to prepare chitin and compound protein powder, preparation method's step is:
(1) raw material homogenate: with shrimp head, shrimp shell, the homogenate of shrimp tail discarded in the prawn processing;
(2) compound microorganism ferments: select the compound microorganism ferments of lactobacillus bulgaricus and subtilis, the quantitative proportion of lactobacillus bulgaricus and subtilis is 1: 2, and inoculum size is 10% of raw material cumulative volume;
(3) chitin preparation: above-mentioned fermented liquid is carried out solid-liquid separation with gauze, and the solid matter drying is pulverized and is namely got chitin;
(4) compound protein powder preparation: fermented liquid is adjusted pH to 7, add the powdery dregs of beans according to the solid-to-liquid ratio ratio of 1: 1, the subtilis that access subsequently accounts for raw material cumulative volume 5% carries out solid fermentation, and the 48h post-drying namely gets compound protein powder.
And the fermentation condition in described step (2) is: 40 ℃ of leavening temperatures, and fermentation time 48h, solid-to-liquid ratio is 1: 2.5.
Advantage of the present invention and positively effect are:
1, the present invention selects lactobacillus bulgaricus and subtilis, utilize their strong acid producing ability and enzymatic productivity to process the prawn tankage, the protein and the calcium carbonate that remove on the shrimp shell prepare chitin, and shrimp shell deproteinizing rate reaches 82-85%, and the decalcification rate reaches 85-87%.
2, contain animal proteinum and the solubility calcium that takes off in one time fermentation liquid of the present invention, but because the fermented liquid moisture content is too high, it is excessive that oven dry consumes the energy, so add the bean cake powder adsorption moisture, the access subtilis, carry out the secondary solid fermentation, utilize the ability of the product proteolytic enzyme of subtilis, vegetable-protein is degraded and decomposed the vegetable-protein supressor, prepare at last compound protein powder, the composition of protein powder by analysis, find that protein content reaches 54-56%, the transformation efficiency of dregs of beans peptide reaches 48.76-50.1%, antinutritional factor obviously reduces, phytic acid content decline 89.2%-92.7% wherein, trypsin ihhibitor content decline 54.06%-64.79%, protein content is higher, the little peptide content of functionally active obviously increases, composition of amino acid is reasonable, a kind of good protein source of can yet be regarded as, be expected to part or most of replace imported fish meal, greatly reduce aquaculture cost.
3, the present invention can realize the greenization utilization of prawn tankage, without any the discharge of pollutent, realizes cleaning production, obtains again high value-added product chitin and compound protein powder.
Embodiment
Further illustrate the present invention below in conjunction with specific implementation method, following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
A kind of method of utilizing compound microorganism ferments prawn tankage to prepare chitin and compound protein powder, step is:
(1) raw material homogenate: raw material is directly selected shrimp head, shrimp shell, shrimp tail discarded in present prawn process for processing, with its homogenate;
(2) compound microorganism ferments: select the compound microorganism ferments of lactobacillus bulgaricus and subtilis, the quantitative proportion of lactobacillus bulgaricus and subtilis is 1: 2, and inoculum size is 10% of raw material cumulative volume; Fermentation condition is: 40 ℃ of leavening temperatures, and fermentation time 48h, solid-to-liquid ratio is 1: 2.5, the system degree of hydrolysis can reach 24.42%-25.81% with this understanding;
(3) chitin preparation: above-mentioned fermented liquid is carried out solid-liquid separation with gauze, and the solid matter drying is pulverized and is namely got chitin.Through check, shrimp shell deproteinizing rate reaches 82-85%, and the decalcification rate reaches 85-87%.
(4) compound protein powder preparation: fermented liquid is adjusted pH to 7 with sodium hydroxide, add the powdery dregs of beans according to the solid-to-liquid ratio ratio of 1: 1, the subtilis that access subsequently accounts for as raw material cumulative volume 5% carries out solid fermentation, and the 48h post-drying namely gets compound protein powder.
The composition of protein powder by analysis, protein content reaches 54-56%, and the transformation efficiency of dregs of beans peptide reaches 48.76-50.1%, and antinutritional factor obviously reduces, phytic acid content decline 89.2%-92.7% wherein, trypsin ihhibitor content decline 54.06%-64.79%.
Claims (2)
1. method of utilizing compound microorganism ferments prawn tankage to prepare chitin and compound protein powder, it is characterized in that: preparation method's step is:
(1) raw material homogenate: with shrimp head, shrimp shell, the homogenate of shrimp tail discarded in the prawn processing;
(2) compound microorganism ferments: select the compound microorganism ferments of lactobacillus bulgaricus and subtilis, the quantitative proportion of lactobacillus bulgaricus and subtilis is 1: 2, and inoculum size is 10% of raw material cumulative volume;
(3) chitin preparation: above-mentioned fermented liquid is carried out solid-liquid separation with gauze, and the solid matter drying is pulverized and is namely got chitin;
(4) compound protein powder preparation: fermented liquid is adjusted pH to 7, add the powdery dregs of beans according to the solid-to-liquid ratio ratio of 1: 1, the subtilis that access subsequently accounts for raw material cumulative volume 5% carries out solid fermentation, and the 48h post-drying namely gets compound protein powder.
2. the method for utilizing compound microorganism ferments prawn tankage to prepare chitin and compound protein powder according to claim 1, it is characterized in that: the fermentation condition in described step (2) is: 40 ℃ of leavening temperatures, fermentation time 48h, solid-to-liquid ratio is 1: 2.5.
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104357361A (en) * | 2014-11-14 | 2015-02-18 | 青岛农业大学 | Bacillus subtilis capable of producing chitinase and application thereof |
| CN104381605A (en) * | 2014-11-06 | 2015-03-04 | 中山市巴斯德农业科技有限公司 | Microbial feed additive and preparation method thereof |
| CN104762294A (en) * | 2015-04-03 | 2015-07-08 | 天津高蜚生物科技发展有限公司 | Preparation method and application of bacillus subtilis mutant strain with high protease yield |
| CN105595243A (en) * | 2015-12-18 | 2016-05-25 | 湖北工业大学 | Lipid-lowering porous fruit and vegetable powder containing rich chitosan and dietary fiber |
| CN106520894A (en) * | 2016-10-24 | 2017-03-22 | 山东维尼莱生物科技股份有限公司 | Method for simultaneously preparing astaxanthin and chitosan by utilization of shrimp waste |
| CN106684417A (en) * | 2016-11-16 | 2017-05-17 | 荣成众智海洋生物科技有限公司 | Microbial fuel cell for synchronously producing electricity and chitosan |
| CN107484882A (en) * | 2017-09-12 | 2017-12-19 | 江苏纳克生物工程有限公司 | A kind of method and its application of waste of aquatic harmless treatment |
| CN108060194A (en) * | 2017-12-23 | 2018-05-22 | 青岛麦迪尔生物工程有限公司 | A kind of method that antioxidation polypeptide and chitin are prepared by the shrimp and crab shells that ferment |
| CN109198176A (en) * | 2018-08-13 | 2019-01-15 | 浙江大学 | A kind of method that shrimp shell solid state fermentation prepares culture of microorganism |
| CN109837224A (en) * | 2018-06-06 | 2019-06-04 | 长沙学院 | Cray processing waste microbial fermentation preparation and its preparation method and application |
| CN110128568A (en) * | 2019-05-21 | 2019-08-16 | 扬州日兴生物科技股份有限公司 | A method of discarded shrimp and crab shells chitin extraction is handled using acetyl Exiguobacterium sp |
| CN110156913A (en) * | 2019-05-21 | 2019-08-23 | 扬州日兴生物科技股份有限公司 | A method of discarded shrimp and crab shells chitin extraction is handled using bacillus cereus |
| CN110157760A (en) * | 2019-03-22 | 2019-08-23 | 温州科技职业学院 | A kind of method of the shrimp shell enzymatic hydrolysis of calcium-ion-binding ability in raising shrimp shell |
| CN110862465A (en) * | 2019-11-26 | 2020-03-06 | 中国科学院海洋研究所 | Method for extracting chitin from litopenaeus vannamei shells |
| CN110903412A (en) * | 2019-12-17 | 2020-03-24 | 山东人和集团有限公司 | Preparation method of chitin by using self-fatty acid decalcification |
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Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104381605A (en) * | 2014-11-06 | 2015-03-04 | 中山市巴斯德农业科技有限公司 | Microbial feed additive and preparation method thereof |
| CN104357361B (en) * | 2014-11-14 | 2015-06-03 | 青岛农业大学 | Bacillus subtilis capable of producing chitinase and application thereof |
| CN104357361A (en) * | 2014-11-14 | 2015-02-18 | 青岛农业大学 | Bacillus subtilis capable of producing chitinase and application thereof |
| CN104762294A (en) * | 2015-04-03 | 2015-07-08 | 天津高蜚生物科技发展有限公司 | Preparation method and application of bacillus subtilis mutant strain with high protease yield |
| CN105595243A (en) * | 2015-12-18 | 2016-05-25 | 湖北工业大学 | Lipid-lowering porous fruit and vegetable powder containing rich chitosan and dietary fiber |
| CN106520894B (en) * | 2016-10-24 | 2019-09-27 | 山东维尼莱生物科技股份有限公司 | A method of astaxanthin, chitosan are produced simultaneously using shrimp waste material |
| CN106520894A (en) * | 2016-10-24 | 2017-03-22 | 山东维尼莱生物科技股份有限公司 | Method for simultaneously preparing astaxanthin and chitosan by utilization of shrimp waste |
| CN106684417A (en) * | 2016-11-16 | 2017-05-17 | 荣成众智海洋生物科技有限公司 | Microbial fuel cell for synchronously producing electricity and chitosan |
| CN107484882A (en) * | 2017-09-12 | 2017-12-19 | 江苏纳克生物工程有限公司 | A kind of method and its application of waste of aquatic harmless treatment |
| CN107484882B (en) * | 2017-09-12 | 2020-03-17 | 江苏纳克生物工程有限公司 | Harmless treatment method for aquatic waste and application thereof |
| CN108060194A (en) * | 2017-12-23 | 2018-05-22 | 青岛麦迪尔生物工程有限公司 | A kind of method that antioxidation polypeptide and chitin are prepared by the shrimp and crab shells that ferment |
| CN109837224A (en) * | 2018-06-06 | 2019-06-04 | 长沙学院 | Cray processing waste microbial fermentation preparation and its preparation method and application |
| CN109198176A (en) * | 2018-08-13 | 2019-01-15 | 浙江大学 | A kind of method that shrimp shell solid state fermentation prepares culture of microorganism |
| CN110157760A (en) * | 2019-03-22 | 2019-08-23 | 温州科技职业学院 | A kind of method of the shrimp shell enzymatic hydrolysis of calcium-ion-binding ability in raising shrimp shell |
| CN110128568A (en) * | 2019-05-21 | 2019-08-16 | 扬州日兴生物科技股份有限公司 | A method of discarded shrimp and crab shells chitin extraction is handled using acetyl Exiguobacterium sp |
| CN110156913A (en) * | 2019-05-21 | 2019-08-23 | 扬州日兴生物科技股份有限公司 | A method of discarded shrimp and crab shells chitin extraction is handled using bacillus cereus |
| CN110862465A (en) * | 2019-11-26 | 2020-03-06 | 中国科学院海洋研究所 | Method for extracting chitin from litopenaeus vannamei shells |
| CN110903412A (en) * | 2019-12-17 | 2020-03-24 | 山东人和集团有限公司 | Preparation method of chitin by using self-fatty acid decalcification |
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Application publication date: 20130605 |