CN108192939A - A kind of preparation method of the yellow mushroom fish collagen of Japan - Google Patents
A kind of preparation method of the yellow mushroom fish collagen of Japan Download PDFInfo
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- CN108192939A CN108192939A CN201810049069.1A CN201810049069A CN108192939A CN 108192939 A CN108192939 A CN 108192939A CN 201810049069 A CN201810049069 A CN 201810049069A CN 108192939 A CN108192939 A CN 108192939A
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 33
- 108010035532 Collagen Proteins 0.000 title claims abstract description 33
- 229920001436 collagen Polymers 0.000 title claims abstract description 33
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 29
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 17
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 15
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 11
- 108091005804 Peptidases Proteins 0.000 claims abstract description 10
- 239000004365 Protease Substances 0.000 claims abstract description 10
- 239000003513 alkali Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 8
- 239000002253 acid Substances 0.000 claims abstract description 7
- 150000001875 compounds Chemical class 0.000 claims abstract description 7
- 238000000605 extraction Methods 0.000 claims abstract description 7
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 6
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 6
- 102000057297 Pepsin A Human genes 0.000 claims abstract description 5
- 108090000284 Pepsin A Proteins 0.000 claims abstract description 5
- 229940088598 enzyme Drugs 0.000 claims abstract description 5
- 229940111202 pepsin Drugs 0.000 claims abstract description 5
- 102000004190 Enzymes Human genes 0.000 claims abstract description 4
- 108090000790 Enzymes Proteins 0.000 claims abstract description 4
- 102000035195 Peptidases Human genes 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims abstract description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000011084 recovery Methods 0.000 abstract description 5
- 238000004140 cleaning Methods 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 239000012620 biological material Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 206010064097 avian influenza Diseases 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 208000033065 inborn errors of immunity Diseases 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 208000028529 primary immunodeficiency disease Diseases 0.000 description 1
- 230000003946 protein process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cosmetics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
A kind of preparation method of the yellow mushroom fish collagen of Japan, the preparation method include the following steps：a）Japanese Huang mushroom fish fish-skin pretreatment：Fish skin powder will be made respectively after drying, crushing after the fish-skin sorting removal of impurities of Japanese Huang mushroom fish, cleaning, then using acid processing decalcification, the non-collagen foreign protein in peeling fish skin is removed using alkali process；b）The enzymatic treatment of fish-skin：The enzymatic treatment condition of fish-skin is：Using 0.5mol/L compound proteases or pepsin, acetic acid solution as extractant, enzyme amount 4000U/g, 35-40 DEG C of Extracting temperature, extraction time is 4-8h, solid-to-liquid ratio 1:4；c）Decoloration：Enzymolysis liquid is dried to obtain Japanese yellow mushroom fish collagen through centrifuging, filtering, decolourize, after defishying；It has preparation process simple, and collagen recovery rate is high, and resource utilization is high, the features such as increasing economic efficiency.
Description
Technical field
The present invention relates to a kind of preparation methods of the yellow mushroom fish collagen of Japan, belong to biochemical product technology neck
Domain.
Background technology
Collagen is a kind of high molecular functional protein, it is the chief component of skin, accounts for dermal layer of the skin
80%.Its unique triple-helix structure, assigns the good characteristics such as its film-formability, low immunogenicity, in food, medicine, U.S.
The fields such as appearance, biomaterial have critical role.There are a large amount of collagen in mammalian body, for a long time, industrially
The extraction raw material of collagen is largely from pigskin, ox-hide etc., but due to the shadow of the livestock contagious diseases such as aftosa, bird flu
It rings, furthermore the religious belief of certain areas also has repulsion psychology to the collagen in the source.Therefore, the collagen egg of marine source
Bai Yin has many advantages, such as hypoimmunity, low thermal denaturation temperature, has good hair in medicine, biomaterial and beauty treatment fields
Open up direction.
Invention content
It is an object of the invention to overcome the shortcomings of the prior art, and it is simple to provide a kind of preparation process, collagen egg
White recovery rate is high, and resource utilization is high, the preparation method of the yellow mushroom fish collagen of the Japan to increase economic efficiency.
The purpose of the present invention is by following technical solution to complete, a kind of system of the yellow mushroom fish collagen of Japan
Preparation Method, the preparation method include the following steps：
a）Japanese Huang mushroom fish fish-skin pretreatment：After the fish-skin sorting of Japanese Huang mushroom fish is cleaned, is cleaned after drying, crushing respectively
Fish skin powder is made, then using acid processing decalcification, using alkali process except the non-collagen foreign protein in peeling fish skin；
b）The enzymatic treatment of fish-skin：The enzymatic treatment condition of fish-skin is：It is molten with 0.5mol/L compound proteases or pepsin, acetic acid
Liquid is as extractant, 4000 U/g of enzyme amount, 35-40 DEG C of Extracting temperature, and extraction time is 4-8h, solid-to-liquid ratio 1:4 ；
c）Decoloration：Enzymolysis liquid is dried to obtain Japanese yellow mushroom fish collagen through centrifuging, filtering, decolourize, after defishying.
As preferred：The step a）In, the drying is crushed under the conditions of 65-80 DEG C, to be dried to moisture
Content≤12wt% smashes it through 30 mesh sieve；The acid reaction variables are：HCl concentration 0.4mol/L, solid-liquid ratio 1:20, it takes off
4-8h of calcium time；Alkali process condition is：NaOH concentration 0.15mol/L, solid-liquid ratio l:20,10-15h of processing time；
The step b）In, during fish-skin enzymatic treatment, the compound protease presses quality for trypsase and alkali protease
Compare 5-6:1 mixes.
The present invention is by the way that Japanese yellow mushroom fish fish-skin is removed foreign protein, degreasing, then first with 5% acetic acid combination pepsin
Collagen is extracted, Japanese yellow mushroom fish collagen is obtained after centrifugal drying.Collagen recovery rate is high, can be effectively sharp
It with discarded collagen, increases economic efficiency, the collagen of extraction heals available for treat wound.
The present invention has preparation process simple, and collagen recovery rate is high, and resource utilization is high, increases economic efficiency and waits spies
Point.
Specific embodiment
Below in conjunction with specific embodiment, the present invention will be described in detail.
Embodiment：A kind of preparation method of the yellow mushroom fish collagen of Japan of the present invention, the preparation side
Method includes the following steps：
a）Japanese Huang mushroom fish fish-skin pretreatment：After the fish-skin sorting of Japanese Huang mushroom fish is cleaned, is cleaned after drying, crushing respectively
Fish skin powder is made, then using acid processing decalcification, using alkali process except the non-collagen foreign protein in peeling fish skin；
b）The enzymatic treatment of fish-skin：The enzymatic treatment condition of fish-skin is：It is molten with 0.5mol/L compound proteases or pepsin, acetic acid
Liquid is as extractant, 4000 U/g of enzyme amount, 35-40 DEG C of Extracting temperature, and extraction time is 4-8h, solid-to-liquid ratio 1:4 ；
c）Decoloration：Enzymolysis liquid is dried to obtain Japanese yellow mushroom fish collagen through centrifuging, filtering, decolourize, after defishying.
As a kind of preferred embodiment：A kind of preparation side of the yellow mushroom fish collagen of Japan of the present invention
Method, the preparation method include the following steps：
The step a）In, the drying is crushed under the conditions of 65-80 DEG C, to be dried to moisture≤12wt%,
Smash it through 30 mesh sieve；The acid reaction variables are：HCl concentration 0.4mol/L, solid-liquid ratio 1:20,4-8h of decalcification time；
Alkali process condition is：NaOH concentration 0.15mol/L, solid-liquid ratio l:20,10-15h of processing time；
The step b）In, during fish-skin enzymatic treatment, the compound protease presses quality for trypsase and alkali protease
Compare 5-6:1 mixes.
It is other identical with embodiment.
Mainly using acid-enzyme hydrolysis method, extraction prepares collagen to the present invention from Japanese yellow mushroom fish fish-skin；The collagen extracted
Protein Process is excellent, and recovery rate is up to more than 85%.
Other embodiments of the invention can carry out simple replacement numerically on the basis of above-described embodiment, can obtain
Countless embodiments, and those skilled in the art can be advantageously carried out the present invention on the basis of the present invention is understood.
Claims (2)
1. a kind of preparation method of the yellow mushroom fish collagen of Japan, it is characterised in that the preparation method includes following step
Suddenly：
a）Japanese Huang mushroom fish fish-skin pretreatment：After the fish-skin sorting of Japanese Huang mushroom fish is cleaned, is cleaned after drying, crushing respectively
Fish skin powder is made, then using acid processing decalcification, using alkali process except the non-collagen foreign protein in peeling fish skin；
b）The enzymatic treatment of fish-skin：The enzymatic treatment condition of fish-skin is：It is molten with 0.5mol/L compound proteases or pepsin, acetic acid
Liquid is as extractant, 4000 U/g of enzyme amount, 35-40 DEG C of Extracting temperature, and extraction time is 4-8h, solid-to-liquid ratio 1:4 ；
c）Decoloration：Enzymolysis liquid is dried to obtain Japanese yellow mushroom fish collagen through centrifuging, filtering, decolourize, after defishying.
2. the preparation method of the yellow mushroom fish collagen of Japan according to claim 1, it is characterised in that：
The step a）In, the drying is crushed under the conditions of 65-80 DEG C, to be dried to moisture≤12wt%,
Smash it through 30 mesh sieve；The acid reaction variables are：HCl concentration 0.4mol/L, solid-liquid ratio 1:20,4-8h of decalcification time；
Alkali process condition is：NaOH concentration 0.15mol/L, solid-liquid ratio l:20,10-15h of processing time；
The step b）In, during fish-skin enzymatic treatment, the compound protease presses quality for trypsase and alkali protease
Compare 5-6:1 mixes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810049069.1A CN108192939A (en) | 2018-01-18 | 2018-01-18 | A kind of preparation method of the yellow mushroom fish collagen of Japan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810049069.1A CN108192939A (en) | 2018-01-18 | 2018-01-18 | A kind of preparation method of the yellow mushroom fish collagen of Japan |
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| Publication Number | Publication Date |
|---|---|
| CN108192939A true CN108192939A (en) | 2018-06-22 |
Family
ID=62589641
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810049069.1A Pending CN108192939A (en) | 2018-01-18 | 2018-01-18 | A kind of preparation method of the yellow mushroom fish collagen of Japan |
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| Country | Link |
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| CN (1) | CN108192939A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110074356A (en) * | 2019-06-04 | 2019-08-02 | 江南大学 | A kind of Java tilapia skin deliming degreasing method |
| CN108728509B (en) * | 2018-08-09 | 2021-07-13 | 浙江海洋大学 | Preparation method of fish skin immunoregulation peptide of Nibea japonica |
| JP2023530376A (en) * | 2021-04-14 | 2023-07-18 | ハイナン サンユアンシィン バイオテクノロジー カンパニー リミテッド | Method for producing collagen peptide |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974588A (en) * | 2010-09-07 | 2011-02-16 | 青岛海健堂生物科技有限公司 | Method for preparing fish collagens |
| CN102242172A (en) * | 2010-05-10 | 2011-11-16 | 上海海健堂生物科技有限公司 | Method for extracting fish collagen from fish skin |
| CN103740793A (en) * | 2013-12-05 | 2014-04-23 | 延边海娇生物科技有限公司 | Method for producing fish skin collagen |
| CN105087734A (en) * | 2015-09-17 | 2015-11-25 | 福建福铭食品有限公司 | Method for preparing collagen powder from fish skin and fish scales |
-
2018
- 2018-01-18 CN CN201810049069.1A patent/CN108192939A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242172A (en) * | 2010-05-10 | 2011-11-16 | 上海海健堂生物科技有限公司 | Method for extracting fish collagen from fish skin |
| CN101974588A (en) * | 2010-09-07 | 2011-02-16 | 青岛海健堂生物科技有限公司 | Method for preparing fish collagens |
| CN103740793A (en) * | 2013-12-05 | 2014-04-23 | 延边海娇生物科技有限公司 | Method for producing fish skin collagen |
| CN105087734A (en) * | 2015-09-17 | 2015-11-25 | 福建福铭食品有限公司 | Method for preparing collagen powder from fish skin and fish scales |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728509B (en) * | 2018-08-09 | 2021-07-13 | 浙江海洋大学 | Preparation method of fish skin immunoregulation peptide of Nibea japonica |
| CN110074356A (en) * | 2019-06-04 | 2019-08-02 | 江南大学 | A kind of Java tilapia skin deliming degreasing method |
| JP2023530376A (en) * | 2021-04-14 | 2023-07-18 | ハイナン サンユアンシィン バイオテクノロジー カンパニー リミテッド | Method for producing collagen peptide |
| JP7401932B2 (en) | 2021-04-14 | 2023-12-20 | ハイナン サンユアンシィン バイオテクノロジー カンパニー リミテッド | Method for producing collagen peptide |
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Application publication date: 20180622 |