CN109645211A - The extracting method of collagen in a kind of ox-hide - Google Patents
The extracting method of collagen in a kind of ox-hide Download PDFInfo
- Publication number
- CN109645211A CN109645211A CN201710943977.0A CN201710943977A CN109645211A CN 109645211 A CN109645211 A CN 109645211A CN 201710943977 A CN201710943977 A CN 201710943977A CN 109645211 A CN109645211 A CN 109645211A
- Authority
- CN
- China
- Prior art keywords
- collagen
- hide
- extracting method
- crude extract
- acidification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000008186 Collagen Human genes 0.000 title claims abstract description 45
- 108010035532 Collagen Proteins 0.000 title claims abstract description 45
- 229920001436 collagen Polymers 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 30
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 18
- 239000000287 crude extract Substances 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 230000020477 pH reduction Effects 0.000 claims abstract description 14
- 108090000790 Enzymes Proteins 0.000 claims abstract description 12
- 102000004190 Enzymes Human genes 0.000 claims abstract description 12
- 238000001704 evaporation Methods 0.000 claims abstract description 9
- 230000008020 evaporation Effects 0.000 claims abstract description 9
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 9
- 235000017557 sodium bicarbonate Nutrition 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000006243 chemical reaction Methods 0.000 claims abstract description 5
- 238000001976 enzyme digestion Methods 0.000 claims abstract description 5
- 238000004108 freeze drying Methods 0.000 claims abstract description 5
- 230000002779 inactivation Effects 0.000 claims abstract description 5
- 230000001376 precipitating effect Effects 0.000 claims abstract description 5
- 238000010298 pulverizing process Methods 0.000 claims abstract description 5
- 239000000758 substrate Substances 0.000 claims abstract description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 229940088598 enzyme Drugs 0.000 claims description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 8
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 238000005554 pickling Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 108010059892 Cellulase Proteins 0.000 claims description 4
- 102000004882 Lipase Human genes 0.000 claims description 4
- 108090001060 Lipase Proteins 0.000 claims description 4
- 239000004367 Lipase Substances 0.000 claims description 4
- 239000000654 additive Substances 0.000 claims description 4
- 230000000996 additive effect Effects 0.000 claims description 4
- 102000005840 alpha-Galactosidase Human genes 0.000 claims description 4
- 108010030291 alpha-Galactosidase Proteins 0.000 claims description 4
- 229940106157 cellulase Drugs 0.000 claims description 4
- 238000004042 decolorization Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 235000019421 lipase Nutrition 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 2
- 108091005804 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 6
- 239000000284 extract Substances 0.000 abstract description 2
- 239000002253 acid Substances 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000003513 alkali Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/10—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from hair, feathers, horn, skins, leather, bones, or the like
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of extracting methods of collagen in ox-hide.The extracting method ox-hide is cleaned and dried first after, acidification is carried out after Ultrasonic Pulverization, sodium bicarbonate is first added in the ox-hide after acidification and is neutralized, then with clear water impregnate 3~5h, centrifuging and taking precipitating, sterilize to obtain collagen crude extract；It is 8~9 by collagen crude extract tune pH, is configured to the substrate solution that mass concentration is 100g/L~160g/L, the complex enzyme of 50~100PPm is added, enzymolysis liquid is made in enzyme digestion reaction 8h~11h at 45 DEG C~60 DEG C；By enzymolysis liquid after inactivation treatment, be concentrated by evaporation freeze-drying, crush to get.The extracting method of collagen in a kind of ox-hide provided by the invention, the extraction efficiency of collagen is higher, and the collagen color and purity that extract are higher, without peculiar smell.
Description
Technical field
The present invention relates to a kind of extracting methods of collagen in the extracting method of collagen, more particularly to ox-hide.
Background technique
Collagen has been more and more widely used in fields such as cosmetics, medicine, food, feeds, and demand is year by year
Increase.At present in Animal Skin collagen extraction process, generally Animal Skin is first marinated through grey alkali, after liming, is turning
Clean deliming with a large amount of clear water in drum, carried out again after deliming pickling cleaning and acidification expansion, with reinforcing fibre interstitial remove and into
One step disperses collagenous fibres, is convenient for subsequent collagen extraction.In acidification technique, ox-hide carries out adding acid for many times, row in rotary drum
Acid, until ox-hide acidification is thorough, acid used is hydrochloric acid.After acidification, carries out washing acid, i.e., ox-hide is cleaned multiple times with clear water, with
Reach the pH requirement of production technology.But the technique has a problem that, in uneven thickness due to ox-hide, and thin skin terminates washing acid
After find to have washed rotten, collagenous fibres seriously destroy, and part collagenous fibres, which have entered, to be washed in acid solution, and thin skin collagen is caused to mention
Take the reduction of rate.
Summary of the invention
In order to reach solution problems of the prior art, the present invention provides a kind of extractions of collagen in ox-hide
The extraction efficiency of method, collagen is higher.
In order to achieve the above object, The technical solution adopted by the invention is as follows:
The extracting method of collagen in a kind of ox-hide, the extracting method the following steps are included:
(1) pretreatment of raw material: after ox-hide is cleaned and dried, through Ultrasonic Pulverization；
(2) acidification: smashed ox-hide is washed and is filtered after pickling；
(3) it washes sour processing: sodium bicarbonate is first added in the ox-hide powder after step (2) acidification and is neutralized, then uses clear water
3~5h is impregnated, centrifuging and taking precipitating, sterilize to obtain collagen crude extract；
(4) purifying of collagenous fibres: by collagen crude extract tune pH be 8~9, be configured to mass concentration be 100g/L~
The substrate solution of 160g/L, is added the complex enzyme of 50~100PPm, and enzymatic hydrolysis is made in enzyme digestion reaction 8h~11h at 45 DEG C~60 DEG C
Liquid；
(5) preparation of collagen: by enzymolysis liquid after inactivation treatment, be concentrated by evaporation freeze-drying, crush to get.
Further, the volume ratio of step (3) sodium bicarbonate and ox-hide powder is 6:9.
Further, the step (3) sterilizing uses moist hear heat test, and sterilising temp is 120 DEG C~125 DEG C, when sterilizing
Between be 20min~35min.
Further, complex enzyme includes cellulase, alpha-galactosidase, mannonase albumen in the step (4)
Enzyme and lipase.
Further, collagen crude extract pH is adjusted using food-grade NaOH in the step (4).
It further, further include decoloration and filtration step before the step (5) is concentrated by evaporation.
Further, the decolorization process specifically uses active carbon decoloring, and the additive amount of active carbon is enzymolysis liquid quality
3%~5%.
The utility model has the advantages that compared with prior art, the extracting method of collagen, collagen in a kind of ox-hide provided by the invention
The extraction efficiency of albumen is higher, and the collagen color and purity that extract are higher, without peculiar smell.
Detailed description of the invention
Fig. 1 is preparation method flow diagram described in the embodiment of the present invention.
Specific embodiment
The present invention is further elaborated with embodiment with reference to the accompanying drawings of the specification.
Embodiment 1
The extracting method of collagen in a kind of ox-hide, the extracting method the following steps are included:
(1) pretreatment of raw material: after ox-hide is cleaned and dried, through Ultrasonic Pulverization；
(2) acidification: smashed ox-hide is washed and is filtered after pickling；
(3) it washes sour processing: sodium bicarbonate is first added in the ox-hide powder after step (2) acidification and is neutralized, then uses clear water
3~5h is impregnated, centrifuging and taking precipitating, sterilize to obtain collagen crude extract；
(4) purifying of collagenous fibres: being 8 by collagen crude extract tune pH, is configured to the bottom that mass concentration is 100g/L
The complex enzyme of 50PPm is added in object solution, enzyme digestion reaction 11h at 45 DEG C, and enzymolysis liquid is made；
(5) preparation of collagen: by enzymolysis liquid after inactivation treatment, be concentrated by evaporation freeze-drying, crush to get.
Further, the volume ratio of step (3) sodium bicarbonate and ox-hide powder is 6:9.
Further, step (3) sterilizing uses moist hear heat test, and sterilising temp is 120 DEG C, and sterilization time is
35min。
Further, complex enzyme includes cellulase, alpha-galactosidase, mannonase albumen in the step (4)
Enzyme and lipase.
Further, collagen crude extract pH is adjusted using food-grade NaOH in the step (4).
It further, further include decoloration and filtration step before the step (5) is concentrated by evaporation.
Further, the decolorization process specifically uses active carbon decoloring, and the additive amount of active carbon is enzymolysis liquid quality
3%.
Embodiment 2
The extracting method of collagen in a kind of ox-hide, the extracting method the following steps are included:
(1) pretreatment of raw material: after ox-hide is cleaned and dried, through Ultrasonic Pulverization；
(2) acidification: smashed ox-hide is washed and is filtered after pickling；
(3) it washes sour processing: sodium bicarbonate is first added in the ox-hide powder after step (2) acidification and is neutralized, then uses clear water
3~5h is impregnated, centrifuging and taking precipitating, sterilize to obtain collagen crude extract；
(4) purifying of collagenous fibres: being 9 by collagen crude extract tune pH, is configured to the bottom that mass concentration is 160g/L
The complex enzyme of 100PPm is added in object solution, enzyme digestion reaction 8h at 60 DEG C, and enzymolysis liquid is made；
(5) preparation of collagen: by enzymolysis liquid after inactivation treatment, be concentrated by evaporation freeze-drying, crush to get.
Further, the volume ratio of step (3) sodium bicarbonate and ox-hide powder is 6:9.
Further, step (3) sterilizing uses moist hear heat test, and sterilising temp is 125 DEG C, and sterilization time is
20min。
Further, complex enzyme includes cellulase, alpha-galactosidase, mannonase albumen in the step (4)
Enzyme and lipase.
Further, collagen crude extract pH is adjusted using food-grade NaOH in the step (4).
It further, further include decoloration and filtration step before the step (5) is concentrated by evaporation.
Further, the decolorization process specifically uses active carbon decoloring, and the additive amount of active carbon is enzymolysis liquid quality
5%.
It is the preferred embodiment of the present invention above, it is noted that for those skilled in the art,
Various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also considered as this hair
Bright protection scope.
Claims (7)
1. the extracting method of collagen in a kind of ox-hide, it is characterised in that: the extracting method the following steps are included:
(1) pretreatment of raw material: after ox-hide is cleaned and dried, through Ultrasonic Pulverization；
(2) acidification: smashed ox-hide is washed and is filtered after pickling；
(3) it washes sour processing: sodium bicarbonate is first added in the ox-hide powder after step (2) acidification and is neutralized, then impregnate 3 with clear water
~5h, centrifuging and taking precipitating, sterilize to obtain collagen crude extract；
(4) purifying of collagenous fibres: by collagen crude extract tune pH be 8~9, be configured to mass concentration be 100g/L~
The substrate solution of 160g/L, is added the complex enzyme of 50~100PPm, and enzymatic hydrolysis is made in enzyme digestion reaction 8h~11h at 45 DEG C~60 DEG C
Liquid；
(5) preparation of collagen: by enzymolysis liquid after inactivation treatment, be concentrated by evaporation freeze-drying, crush to get.
2. the extracting method of collagen in a kind of ox-hide according to claim 1, it is characterised in that: the step (3)
The volume ratio of sodium bicarbonate and ox-hide powder is 6:9.
3. the extracting method of collagen in a kind of ox-hide according to claim 1, it is characterised in that: the step (3)
Sterilizing uses moist hear heat test, and sterilising temp is 120 DEG C~125 DEG C, and sterilization time is 20min~35min.
4. the extracting method of collagen in a kind of ox-hide according to claim 1, it is characterised in that: the step (4)
Middle complex enzyme includes cellulase, alpha-galactosidase, mannonase protease and lipase.
5. the extracting method of collagen in a kind of ox-hide according to claim 1, it is characterised in that: the step (4)
It is middle that collagen crude extract pH is adjusted using food-grade NaOH.
6. the extracting method of collagen in a kind of ox-hide according to claim 1, it is characterised in that: the step (5)
It further include decoloration and filtration step before evaporation and concentration.
7. the extracting method of collagen in a kind of ox-hide according to claim 6, it is characterised in that: the decolorization process
Specific to use active carbon decoloring, the additive amount of active carbon is the 3%~5% of enzymolysis liquid quality.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710943977.0A CN109645211A (en) | 2017-10-12 | 2017-10-12 | The extracting method of collagen in a kind of ox-hide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710943977.0A CN109645211A (en) | 2017-10-12 | 2017-10-12 | The extracting method of collagen in a kind of ox-hide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109645211A true CN109645211A (en) | 2019-04-19 |
Family
ID=66109743
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710943977.0A Pending CN109645211A (en) | 2017-10-12 | 2017-10-12 | The extracting method of collagen in a kind of ox-hide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109645211A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113349343A (en) * | 2021-07-09 | 2021-09-07 | 天津科技大学 | Preparation method and application of natural collagen fiber |
| CN113862321A (en) * | 2021-10-11 | 2021-12-31 | 齐鲁工业大学 | Method for extracting nutritional ingredients from cattle hair or cattle hide leftover |
| CN115403669A (en) * | 2022-10-14 | 2022-11-29 | 山东杰乐生物科技有限公司 | Preparation method of ultralow-molecular-weight cow leather collagen |
-
2017
- 2017-10-12 CN CN201710943977.0A patent/CN109645211A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113349343A (en) * | 2021-07-09 | 2021-09-07 | 天津科技大学 | Preparation method and application of natural collagen fiber |
| CN113862321A (en) * | 2021-10-11 | 2021-12-31 | 齐鲁工业大学 | Method for extracting nutritional ingredients from cattle hair or cattle hide leftover |
| CN115403669A (en) * | 2022-10-14 | 2022-11-29 | 山东杰乐生物科技有限公司 | Preparation method of ultralow-molecular-weight cow leather collagen |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190419 |